TAS2R4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TAS2R4
Entrez Gene ID	50832
Full Name	taste 2 receptor member 4
Synonyms	T2R4
General protein information	Preferred Names taste 2 receptor member 4 Names taste receptor type 2 member 4 candidate taste receptor T2R4 taste receptor, type 2, member 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q34
Summary	This gene encodes a member of a family of candidate taste receptors that are members of the G protein-coupled receptor superfamily and that are specifically expressed by taste receptor cells of the tongue and palate epithelia. These apparently intronless genes encode a 7-transmembrane receptor protein, functioning as a bitter taste receptor. This gene is clustered with another 3 candidate taste receptor genes in chromosome 7 and is genetically linked to loci that influence bitter perception. [provided by RefSeq, Jul 2008].
Disorder MIM:	604869

mRNA and Protein(s)

mRNA	Protein	Name
NM_016944.2	NP_058640.1	taste receptor type 2 member 4
NM_016944.1	NP_058640.1	taste receptor type 2 member 4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TAS2R4	NP_001040100.1
Rattus norvegicus (Norway rat)	Tas2r108	NP_001019857.1
Pan troglodytes (chimpanzee)	TAS2R4	NP_001009161.1
	TAS2R4	XP_001084778.1
Canis lupus familiaris (dog)	TAS2R4	XP_003639608.1
Homo sapiens (human)	TAS2R4	NP_058640.1
Mus musculus (house mouse)	Tas2r108	NP_065248.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of Rac1 GTPase activity by quinine through G-protein and bitter taste receptor T2R4.
Sidhu C, Jaggupilli A, Chelikani P, Bhullar RP
Molecular and cellular biochemistry426(1-2)129-136(2017 Feb)

Cholesterol modulates bitter taste receptor function.
Pydi SP, Jafurulla M, Wai L, Bhullar RP, Chelikani P, Chattopadhyay A
Biochimica et biophysica acta1858(9)2081-2087(2016 09)

The structure-function role of C-terminus in human bitter taste receptor T2R4 signaling.
Upadhyaya J, Singh N, Bhullar RP, Chelikani P
Biochimica et biophysica acta1848(7)1502-8(2015 Jul)

Abscisic Acid Acts as a Blocker of the Bitter Taste G Protein-Coupled Receptor T2R4.
Pydi SP, Jaggupilli A, Nelson KM, Abrams SR, Bhullar RP, Loewen MC, Chelikani P
Biochemistry54(16)2622-31(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study is the first to show an inhibitory downstream action of a T2R4 agonist on Rac1 function.
Title: Regulation of Rac1 GTPase activity by quinine through G-protein and bitter taste receptor T2R4.

demonstrate that Lys117, an important CRAC residue, is crucial in the reported cholesterol sensitivity of T2R4. Interestingly, cholesterol sensitivity of T2R4 was observed at quinine concentrations in the lower mM range.
Title: Cholesterol modulates bitter taste receptor function.

Data indicate that KLK/R motif in C-terminus performs functional role in bitter taste receptor T2R4 signaling.
Title: The structure-function role of C-terminus in human bitter taste receptor T2R4 signaling.

The antagonist ability of ABA was tested using another T2R4 agonist, yohimbine. Our results suggest that ABA does not inhibit yohimbine-induced T2R4 activity.
Title: Abscisic Acid Acts as a Blocker of the Bitter Taste G Protein-Coupled Receptor T2R4.

Data indicate that the itter blockers and agonists share the same orthosteric site in bitter taste receptor T2R4.
Title: Amino acid derivatives as bitter taste receptor (T2R) blockers.

The following TAS2R4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TAS2R4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16199	NM_016944.2 Latest version!	Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu16199	NM_016944.1	Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TAS2R4 ( NM_016944.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_058640.1 Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.

Price & Availability↑

CloneID	OHu16199
Clone ID Related Accession (Same CDS sequence)	NM_016944.2 , NM_016944.1
Accession Version	NM_016944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	taste receptor type 2 member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC004979.1. On Nov 22, 2019 this sequence version replaced NM_016944.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR1803615.226850.1, SRR1660805.122680.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTTCGGT TATTCTATTT CTCTGCTATT ATTGCCTCAG TTATTTTAAA TTTTGTAGGA 
ATCATTATGA ATCTGTTTAT TACAGTGGTC AATTGCAAAA CTTGGGTCAA AAGCCATAGA 
ATCTCCTCTT CTGATAGGAT TCTGTTCAGC CTGGGCATCA CCAGGTTTCT TATGCTGGGA 
CTATTTCTGG TGAACACCAT CTACTTCGTC TCTTCAAATA CGGAAAGGTC AGTCTACCTG 
TCTGCTTTTT TTGTGTTGTG TTTCATGTTT TTGGACTCGA GCAGTGTCTG GTTTGTGACC 
TTGCTCAATA TCTTGTACTG TGTGAAGATT ACTAACTTCC AACACTCAGT GTTTCTCCTG 
CTGAAGCGGA ATATCTCCCC AAAGATCCCC AGGCTGCTGC TGGCCTGTGT GCTGATTTCT 
GCTTTCACCA CTTGCCTGTA CATCACGCTT AGCCAGGCAT CACCTTTTCC TGAACTTGTG 
ACTACGAGAA ATAACACATC ATTTAATATC AGTGAGGGCA TCTTGTCTTT AGTGGTTTCT 
TTGGTCTTGA GCTCATCTCT CCAGTTCATC ATTAATGTGA CTTCTGCTTC CTTGCTAATA 
CACTCCTTGA GGAGACATAT ACAGAAGATG CAGAAAAATG CCACTGGTTT CTGGAATCCC 
CAGACGGAAG CTCATGTAGG TGCTATGAAG CTGATGGTCT ATTTCCTCAT CCTCTACATT 
CCATATTCAG TTGCTACCCT GGTCCAGTAT CTCCCCTTTT ATGCAGGGAT GGATATGGGG 
ACCAAATCCA TTTGTCTGAT TTTTGCCACC CTTTACTCTC CAGGACATTC TGTTCTCATT 
ATTATCACAC ATCCTAAACT GAAAACAACA GCAAAGAAGA TTCTTTGTTT CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058640.1
CDS	1816..2715
Translation	MLRLFYFSAIIASVILNFVGIIMNLFITVVNCKTWVKSHRISSSDRILFSLGITRFLMLGLFLVNTIYFVSSNTERSVYLSAFFVLCFMFLDSSSVWFVTLLNILYCVKITNFQHSVFLLLKRNISPKIPRLLLACVLISAFTTCLYITLSQASPFPELVTTRNNTSFNISEGILSLVVSLVLSSSLQFIINVTSASLLIHSLRRHIQKMQKNATGFWNPQTEAHVGAMKLMVYFLILYIPYSVATLVQYLPFYAGMDMGTKSICLIFATLYSPGHSVLIIITHPKLKTTAKKILCFKK

Target ORF information:

RefSeq Version	NM_016944.2
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016944.2

ORF Insert Sequence:

ATGCTTCGGT TATTCTATTT CTCTGCTATT ATTGCCTCAG TTATTTTAAA TTTTGTAGGA 
ATCATTATGA ATCTGTTTAT TACAGTGGTC AATTGCAAAA CTTGGGTCAA AAGCCATAGA 
ATCTCCTCTT CTGATAGGAT TCTGTTCAGC CTGGGCATCA CCAGGTTTCT TATGCTGGGA 
CTATTTCTGG TGAACACCAT CTACTTCGTC TCTTCAAATA CGGAAAGGTC AGTCTACCTG 
TCTGCTTTTT TTGTGTTGTG TTTCATGTTT TTGGACTCGA GCAGTGTCTG GTTTGTGACC 
TTGCTCAATA TCTTGTACTG TGTGAAGATT ACTAACTTCC AACACTCAGT GTTTCTCCTG 
CTGAAGCGGA ATATCTCCCC AAAGATCCCC AGGCTGCTGC TGGCCTGTGT GCTGATTTCT 
GCTTTCACCA CTTGCCTGTA CATCACGCTT AGCCAGGCAT CACCTTTTCC TGAACTTGTG 
ACTACGAGAA ATAACACATC ATTTAATATC AGTGAGGGCA TCTTGTCTTT AGTGGTTTCT 
TTGGTCTTGA GCTCATCTCT CCAGTTCATC ATTAATGTGA CTTCTGCTTC CTTGCTAATA 
CACTCCTTGA GGAGACATAT ACAGAAGATG CAGAAAAATG CCACTGGTTT CTGGAATCCC 
CAGACGGAAG CTCATGTAGG TGCTATGAAG CTGATGGTCT ATTTCCTCAT CCTCTACATT 
CCATATTCAG TTGCTACCCT GGTCCAGTAT CTCCCCTTTT ATGCAGGGAT GGATATGGGG 
ACCAAATCCA TTTGTCTGAT TTTTGCCACC CTTTACTCTC CAGGACATTC TGTTCTCATT 
ATTATCACAC ATCCTAAACT GAAAACAACA GCAAAGAAGA TTCTTTGTTT CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TAS2R4 ( NM_016944.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_058640.1 Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.

Price & Availability↑

CloneID	OHu16199
Clone ID Related Accession (Same CDS sequence)	NM_016944.2 , NM_016944.1
Accession Version	NM_016944.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	taste receptor type 2 member 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF227131.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGCTTCGGT TATTCTATTT CTCTGCTATT ATTGCCTCAG TTATTTTAAA TTTTGTAGGA 
ATCATTATGA ATCTGTTTAT TACAGTGGTC AATTGCAAAA CTTGGGTCAA AAGCCATAGA 
ATCTCCTCTT CTGATAGGAT TCTGTTCAGC CTGGGCATCA CCAGGTTTCT TATGCTGGGA 
CTATTTCTGG TGAACACCAT CTACTTCGTC TCTTCAAATA CGGAAAGGTC AGTCTACCTG 
TCTGCTTTTT TTGTGTTGTG TTTCATGTTT TTGGACTCGA GCAGTGTCTG GTTTGTGACC 
TTGCTCAATA TCTTGTACTG TGTGAAGATT ACTAACTTCC AACACTCAGT GTTTCTCCTG 
CTGAAGCGGA ATATCTCCCC AAAGATCCCC AGGCTGCTGC TGGCCTGTGT GCTGATTTCT 
GCTTTCACCA CTTGCCTGTA CATCACGCTT AGCCAGGCAT CACCTTTTCC TGAACTTGTG 
ACTACGAGAA ATAACACATC ATTTAATATC AGTGAGGGCA TCTTGTCTTT AGTGGTTTCT 
TTGGTCTTGA GCTCATCTCT CCAGTTCATC ATTAATGTGA CTTCTGCTTC CTTGCTAATA 
CACTCCTTGA GGAGACATAT ACAGAAGATG CAGAAAAATG CCACTGGTTT CTGGAATCCC 
CAGACGGAAG CTCATGTAGG TGCTATGAAG CTGATGGTCT ATTTCCTCAT CCTCTACATT 
CCATATTCAG TTGCTACCCT GGTCCAGTAT CTCCCCTTTT ATGCAGGGAT GGATATGGGG 
ACCAAATCCA TTTGTCTGAT TTTTGCCACC CTTTACTCTC CAGGACATTC TGTTCTCATT 
ATTATCACAC ATCCTAAACT GAAAACAACA GCAAAGAAGA TTCTTTGTTT CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058640.1
CDS	1..900
Translation	MLRLFYFSAIIASVILNFVGIIMNLFITVVNCKTWVKSHRISSSDRILFSLGITRFLMLGLFLVNTIYFVSSNTERSVYLSAFFVLCFMFLDSSSVWFVTLLNILYCVKITNFQHSVFLLLKRNISPKIPRLLLACVLISAFTTCLYITLSQASPFPELVTTRNNTSFNISEGILSLVVSLVLSSSLQFIINVTSASLLIHSLRRHIQKMQKNATGFWNPQTEAHVGAMKLMVYFLILYIPYSVATLVQYLPFYAGMDMGTKSICLIFATLYSPGHSVLIIITHPKLKTTAKKILCFKK

Target ORF information:

RefSeq Version	NM_016944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens taste 2 receptor member 4 (TAS2R4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016944.1

ORF Insert Sequence:

ATGCTTCGGT TATTCTATTT CTCTGCTATT ATTGCCTCAG TTATTTTAAA TTTTGTAGGA 
ATCATTATGA ATCTGTTTAT TACAGTGGTC AATTGCAAAA CTTGGGTCAA AAGCCATAGA 
ATCTCCTCTT CTGATAGGAT TCTGTTCAGC CTGGGCATCA CCAGGTTTCT TATGCTGGGA 
CTATTTCTGG TGAACACCAT CTACTTCGTC TCTTCAAATA CGGAAAGGTC AGTCTACCTG 
TCTGCTTTTT TTGTGTTGTG TTTCATGTTT TTGGACTCGA GCAGTGTCTG GTTTGTGACC 
TTGCTCAATA TCTTGTACTG TGTGAAGATT ACTAACTTCC AACACTCAGT GTTTCTCCTG 
CTGAAGCGGA ATATCTCCCC AAAGATCCCC AGGCTGCTGC TGGCCTGTGT GCTGATTTCT 
GCTTTCACCA CTTGCCTGTA CATCACGCTT AGCCAGGCAT CACCTTTTCC TGAACTTGTG 
ACTACGAGAA ATAACACATC ATTTAATATC AGTGAGGGCA TCTTGTCTTT AGTGGTTTCT 
TTGGTCTTGA GCTCATCTCT CCAGTTCATC ATTAATGTGA CTTCTGCTTC CTTGCTAATA 
CACTCCTTGA GGAGACATAT ACAGAAGATG CAGAAAAATG CCACTGGTTT CTGGAATCCC 
CAGACGGAAG CTCATGTAGG TGCTATGAAG CTGATGGTCT ATTTCCTCAT CCTCTACATT 
CCATATTCAG TTGCTACCCT GGTCCAGTAT CTCCCCTTTT ATGCAGGGAT GGATATGGGG 
ACCAAATCCA TTTGTCTGAT TTTTGCCACC CTTTACTCTC CAGGACATTC TGTTCTCATT 
ATTATCACAC ATCCTAAACT GAAAACAACA GCAAAGAAGA TTCTTTGTTT CAAAAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of Rac1 GTPase activity by quinine through G-protein and bitter taste receptor T2R4.
Molecular and cellular biochemistry426(1-2)129-136(2017 Feb)
Sidhu C,Jaggupilli A,Chelikani P,Bhullar RP

Cholesterol modulates bitter taste receptor function.
Biochimica et biophysica acta1858(9)2081-2087(2016 09)
Pydi SP,Jafurulla M,Wai L,Bhullar RP,Chelikani P,Chattopadhyay A

The structure-function role of C-terminus in human bitter taste receptor T2R4 signaling.
Biochimica et biophysica acta1848(7)1502-8(2015 Jul)
Upadhyaya J,Singh N,Bhullar RP,Chelikani P

Abscisic Acid Acts as a Blocker of the Bitter Taste G Protein-Coupled Receptor T2R4.
Biochemistry54(16)2622-31(2015 Apr)
Pydi SP,Jaggupilli A,Nelson KM,Abrams SR,Bhullar RP,Loewen MC,Chelikani P

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TAS2R4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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