RRM2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RRM2B
Entrez Gene ID	50484
Full Name	ribonucleotide reductase regulatory TP53 inducible subunit M2B
Synonyms	MTDPS8A,MTDPS8B,P53R2
General protein information	Preferred Names ribonucleotide reductase regulatory TP53 inducible subunit M2B Names ribonucleoside-diphosphate reductase subunit M2 B TP53-inducible ribonucleotide reductase M2 B p53-inducible ribonucleotide reductase small subunit 2 homolog p53-inducible ribonucleotide reductase small subunit 2 short form beta p53-inducible ribonucleotide reductase small subunit 2-like protein ribonucleotide reductase M2 B (TP53 inducible)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q22.3
Summary	This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010].
Disorder MIM:	604712
Disorder Html:	Mitochondrial DNA depletion syndrome 8A (encephalomyopathic type with renal tubulopathy), 612075 (3); Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant 5, 613077 (3); Mitochondrial DNA depletion syndrome 8B (MNGIE type), 612075 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_015713.4	NP_056528.2	ribonucleoside-diphosphate reductase subunit M2 B isoform 1
NM_001172477.1	NP_001165948.1	ribonucleoside-diphosphate reductase subunit M2 B isoform 2
NM_015713.5	NP_056528.2	ribonucleoside-diphosphate reductase subunit M2 B isoform 1
NM_001172478.2	NP_001165949.1	ribonucleoside-diphosphate reductase subunit M2 B isoform 3
NM_001172478.1	NP_001165949.1	ribonucleoside-diphosphate reductase subunit M2 B isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RRM2B	XP_539109.3
Mus musculus (house mouse)	Rrm2b	NP_955770.1
Homo sapiens (human)	RRM2B	NP_001165948.1
Gallus gallus (chicken)	RRM2B	XP_418364.2
Pan troglodytes (chimpanzee)	RRM2B	XP_001153916.1
Bos taurus (cattle)	RRM2B	XP_002692807.1
Danio rerio (zebrafish)	rrm2b	NP_001007164.1
Arabidopsis thaliana (thale cress)	RNR2A	NP_189000.1
Rattus norvegicus (Norway rat)	Rrm2b	NP_001124015.1
Xenopus tropicalis (tropical clawed frog)	rrm2b	NP_001119973.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of p53R2 is associated with poor prognosis in lung sarcomatoid carcinoma.
Chen J, Xiao Y, Cai X, Liu J, Chen K, Zhang X
BMC cancer17(1)855(2017 Dec)

p53R2 as a novel prognostic biomarker in nasopharyngeal carcinoma.
Chen J, Li S, Xiao Y, Zou X, Zhang X, Zhu M, Cai M, Xie D
BMC cancer17(1)846(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

RRM1/RRM2B enzyme is capable of retaining activity in hypoxia and therefore is favored over RRM1/RRM2 in order to preserve ongoing replication and avoid the accumulation of DNA damage in hypoxic cells.
Title: Ribonucleotide Reductase Requires Subunit Switching in Hypoxia to Maintain DNA Replication.

p53R2 acts as a positive regulator of TrxR2 activity in mitochondria both under normal physiological conditions and during the cellular response to DNA damage
Title: p53R2 regulates thioredoxin reductase activity through interaction with TrxR2.

Silencing of both PYCR1 and PYCR2 completely abolished anti-oxidation activity of RRM2B, demonstrating a functional collaboration of these metabolic enzymes in response to oxidative stress.
Title: PYCR1 and PYCR2 Interact and Collaborate with RRM2B to Protect Cells from Overt Oxidative Stress.

analysis of caspase-dependent degradation of human R2 and p53R2 small subunits
Title: Caspase-dependent Proteolysis of Human Ribonucleotide Reductase Small Subunits R2 and p53R2 during Apoptosis.

A newly discovered role of E2F1 in the regulation of p53R2 expression in DNA damage response.
Title: E2F1 regulates p53R2 gene expression in p53-deficient cells.

The following RRM2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RRM2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26388	NM_015713.5 Latest version!	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26172	NM_001172478.2 Latest version!	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu26188	NM_001172477.1 Latest version!	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
View Old Accession Versions >>
OHu26388	NM_015713.4	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26172	NM_001172478.1	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RRM2B ( NM_015713.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056528.2 Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26388
Clone ID Related Accession (Same CDS sequence)	NM_015713.4 , NM_015713.5
Accession Version	NM_015713.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-06
Organism	Homo sapiens(human)
Product	ribonucleoside-diphosphate reductase subunit M2 B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB036063.1, BC108261.1 and AK023605.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2010 this sequence version replaced NM_015713.3. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB036063.1, SRR1660809.144077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGAG ATCATCTTCA 
GACACCAACG AAAGTGAAAT AAAGTCAAAT GAAGAGCCAC TCCTAAGAAA GAGTTCTCGC 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AAATGTATAA ACAGGCACAG 
GCTTCCTTCT GGACAGCAGA AGAGGTCGAC TTATCAAAGG ATCTCCCTCA CTGGAACAAG 
CTTAAAGCAG ATGAGAAGTA CTTCATCTCT CACATCTTAG CCTTTTTTGC AGCCAGTGAT 
GGAATTGTAA ATGAAAATTT GGTGGAGCGC TTTAGTCAGG AGGTGCAGGT TCCAGAGGCT 
CGCTGTTTCT ATGGCTTTCA AATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTG 
CTGATAGACA CTTACATCAG AGATCCCAAG AAAAGGGAAT TTTTATTTAA TGCAATTGAA 
ACCATGCCCT ATGTTAAGAA AAAAGCAGAT TGGGCCTTGC GATGGATAGC AGATAGAAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTT GCTGCTGTAG AAGGAGTTTT CTTCTCAGGA 
TCTTTTGCTG CTATATTCTG GCTAAAGAAG AGAGGTCTTA TGCCAGGACT CACTTTTTCC 
AATGAACTCA TCAGCAGAGA TGAAGGACTT CACTGTGACT TTGCTTGCCT GATGTTCCAA 
TACTTAGTAA ATAAGCCTTC AGAAGAAAGG GTCAGGGAGA TCATTGTTGA TGCTGTCAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCAGTTGGCC TCATTGGAAT GAATTGCATT 
TTGATGAAAC AGTACATTGA GTTTGTAGCT GACAGATTAC TTGTGGAACT TGGATTCTCA 
AAGGTTTTTC AGGCAGAAAA TCCTTTTGAT TTTATGGAAA ACATTTCTTT AGAAGGAAAA 
ACAAATTTCT TTGAGAAACG AGTTTCAGAG TATCAGCGTT TTGCAGTTAT GGCAGAAACC 
ACAGATAACG TCTTCACCTT GGATGCAGAT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056528.2
CDS	245..1300
Translation	MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_015713.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015713.4

ORF Insert Sequence:

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGAG ATCATCTTCA 
GACACCAACG AAAGTGAAAT AAAGTCAAAT GAAGAGCCAC TCCTAAGAAA GAGTTCTCGC 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AAATGTATAA ACAGGCACAG 
GCTTCCTTCT GGACAGCAGA AGAGGTCGAC TTATCAAAGG ATCTCCCTCA CTGGAACAAG 
CTTAAAGCAG ATGAGAAGTA CTTCATCTCT CACATCTTAG CCTTTTTTGC AGCCAGTGAT 
GGAATTGTAA ATGAAAATTT GGTGGAGCGC TTTAGTCAGG AGGTGCAGGT TCCAGAGGCT 
CGCTGTTTCT ATGGCTTTCA AATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTG 
CTGATAGACA CTTACATCAG AGATCCCAAG AAAAGGGAAT TTTTATTTAA TGCAATTGAA 
ACCATGCCCT ATGTTAAGAA AAAAGCAGAT TGGGCCTTGC GATGGATAGC AGATAGAAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTT GCTGCTGTAG AAGGAGTTTT CTTCTCAGGA 
TCTTTTGCTG CTATATTCTG GCTAAAGAAG AGAGGTCTTA TGCCAGGACT CACTTTTTCC 
AATGAACTCA TCAGCAGAGA TGAAGGACTT CACTGTGACT TTGCTTGCCT GATGTTCCAA 
TACTTAGTAA ATAAGCCTTC AGAAGAAAGG GTCAGGGAGA TCATTGTTGA TGCTGTCAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCAGTTGGCC TCATTGGAAT GAATTGCATT 
TTGATGAAAC AGTACATTGA GTTTGTAGCT GACAGATTAC TTGTGGAACT TGGATTCTCA 
AAGGTTTTTC AGGCAGAAAA TCCTTTTGAT TTTATGGAAA ACATTTCTTT AGAAGGAAAA 
ACAAATTTCT TTGAGAAACG AGTTTCAGAG TATCAGCGTT TTGCAGTTAT GGCAGAAACC 
ACAGATAACG TCTTCACCTT GGATGCAGAT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRM2B ( NM_015713.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_056528.2 Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26388
Clone ID Related Accession (Same CDS sequence)	NM_015713.4 , NM_015713.5
Accession Version	NM_015713.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	ribonucleoside-diphosphate reductase subunit M2 B isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB036063.1, BC108261.1 and AK023605.1. On Aug 31, 2019 this sequence version replaced NM_015713.4. Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB036063.1, AL137348.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGAG ATCATCTTCA 
GACACCAACG AAAGTGAAAT AAAGTCAAAT GAAGAGCCAC TCCTAAGAAA GAGTTCTCGC 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AAATGTATAA ACAGGCACAG 
GCTTCCTTCT GGACAGCAGA AGAGGTCGAC TTATCAAAGG ATCTCCCTCA CTGGAACAAG 
CTTAAAGCAG ATGAGAAGTA CTTCATCTCT CACATCTTAG CCTTTTTTGC AGCCAGTGAT 
GGAATTGTAA ATGAAAATTT GGTGGAGCGC TTTAGTCAGG AGGTGCAGGT TCCAGAGGCT 
CGCTGTTTCT ATGGCTTTCA AATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTG 
CTGATAGACA CTTACATCAG AGATCCCAAG AAAAGGGAAT TTTTATTTAA TGCAATTGAA 
ACCATGCCCT ATGTTAAGAA AAAAGCAGAT TGGGCCTTGC GATGGATAGC AGATAGAAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTT GCTGCTGTAG AAGGAGTTTT CTTCTCAGGA 
TCTTTTGCTG CTATATTCTG GCTAAAGAAG AGAGGTCTTA TGCCAGGACT CACTTTTTCC 
AATGAACTCA TCAGCAGAGA TGAAGGACTT CACTGTGACT TTGCTTGCCT GATGTTCCAA 
TACTTAGTAA ATAAGCCTTC AGAAGAAAGG GTCAGGGAGA TCATTGTTGA TGCTGTCAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCAGTTGGCC TCATTGGAAT GAATTGCATT 
TTGATGAAAC AGTACATTGA GTTTGTAGCT GACAGATTAC TTGTGGAACT TGGATTCTCA 
AAGGTTTTTC AGGCAGAAAA TCCTTTTGAT TTTATGGAAA ACATTTCTTT AGAAGGAAAA 
ACAAATTTCT TTGAGAAACG AGTTTCAGAG TATCAGCGTT TTGCAGTTAT GGCAGAAACC 
ACAGATAACG TCTTCACCTT GGATGCAGAT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056528.2
CDS	88..1143
Translation	MGDPERPEAAGLDQDERSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_015713.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015713.5

ORF Insert Sequence:

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGAG ATCATCTTCA 
GACACCAACG AAAGTGAAAT AAAGTCAAAT GAAGAGCCAC TCCTAAGAAA GAGTTCTCGC 
CGGTTTGTCA TCTTTCCAAT CCAGTACCCT GATATTTGGA AAATGTATAA ACAGGCACAG 
GCTTCCTTCT GGACAGCAGA AGAGGTCGAC TTATCAAAGG ATCTCCCTCA CTGGAACAAG 
CTTAAAGCAG ATGAGAAGTA CTTCATCTCT CACATCTTAG CCTTTTTTGC AGCCAGTGAT 
GGAATTGTAA ATGAAAATTT GGTGGAGCGC TTTAGTCAGG AGGTGCAGGT TCCAGAGGCT 
CGCTGTTTCT ATGGCTTTCA AATTCTCATC GAGAATGTTC ACTCAGAGAT GTACAGTTTG 
CTGATAGACA CTTACATCAG AGATCCCAAG AAAAGGGAAT TTTTATTTAA TGCAATTGAA 
ACCATGCCCT ATGTTAAGAA AAAAGCAGAT TGGGCCTTGC GATGGATAGC AGATAGAAAA 
TCTACTTTTG GGGAAAGAGT GGTGGCCTTT GCTGCTGTAG AAGGAGTTTT CTTCTCAGGA 
TCTTTTGCTG CTATATTCTG GCTAAAGAAG AGAGGTCTTA TGCCAGGACT CACTTTTTCC 
AATGAACTCA TCAGCAGAGA TGAAGGACTT CACTGTGACT TTGCTTGCCT GATGTTCCAA 
TACTTAGTAA ATAAGCCTTC AGAAGAAAGG GTCAGGGAGA TCATTGTTGA TGCTGTCAAA 
ATTGAGCAGG AGTTTTTAAC AGAAGCCTTG CCAGTTGGCC TCATTGGAAT GAATTGCATT 
TTGATGAAAC AGTACATTGA GTTTGTAGCT GACAGATTAC TTGTGGAACT TGGATTCTCA 
AAGGTTTTTC AGGCAGAAAA TCCTTTTGAT TTTATGGAAA ACATTTCTTT AGAAGGAAAA 
ACAAATTTCT TTGAGAAACG AGTTTCAGAG TATCAGCGTT TTGCAGTTAT GGCAGAAACC 
ACAGATAACG TCTTCACCTT GGATGCAGAT TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRM2B ( NM_001172478.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165949.1 Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26172
Clone ID Related Accession (Same CDS sequence)	NM_001172478.2 , NM_001172478.1
Accession Version	NM_001172478.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	ribonucleoside-diphosphate reductase subunit M2 B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB036063.1, BC108261.1 and AK023605.1. On Jun 2, 2019 this sequence version replaced NM_001172478.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.19217.1, SRR1660805.149489.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGGT CGACTTATCA 
AAGGATCTCC CTCACTGGAA CAAGCTTAAA GCAGATGAGA AGTACTTCAT CTCTCACATC 
TTAGCCTTTT TTGCAGCCAG TGATGGAATT GTAAATGAAA ATTTGGTGGA GCGCTTTAGT 
CAGGAGGTGC AGGTTCCAGA GGCTCGCTGT TTCTATGGCT TTCAAATTCT CATCGAGAAT 
GTTCACTCAG AGATGTACAG TTTGCTGATA GACACTTACA TCAGAGATCC CAAGAAAAGG 
GAATTTTTAT TTAATGCAAT TGAAACCATG CCCTATGTTA AGAAAAAAGC AGATTGGGCC 
TTGCGATGGA TAGCAGATAG AAAATCTACT TTTGGGGAAA GAGTGGTGGC CTTTGCTGCT 
GTAGAAGGAG TTTTCTTCTC AGGATCTTTT GCTGCTATAT TCTGGCTAAA GAAGAGAGGT 
CTTATGCCAG GACTCACTTT TTCCAATGAA CTCATCAGCA GAGATGAAGG ACTTCACTGT 
GACTTTGCTT GCCTGATGTT CCAATACTTA GTAAATAAGC CTTCAGAAGA AAGGGTCAGG 
GAGATCATTG TTGATGCTGT CAAAATTGAG CAGGAGTTTT TAACAGAAGC CTTGCCAGTT 
GGCCTCATTG GAATGAATTG CATTTTGATG AAACAGTACA TTGAGTTTGT AGCTGACAGA 
TTACTTGTGG AACTTGGATT CTCAAAGGTT TTTCAGGCAG AAAATCCTTT TGATTTTATG 
GAAAACATTT CTTTAGAAGG AAAAACAAAT TTCTTTGAGA AACGAGTTTC AGAGTATCAG 
CGTTTTGCAG TTATGGCAGA AACCACAGAT AACGTCTTCA CCTTGGATGC AGATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165949.1
CDS	88..987
Translation	MGDPERPEAAGLDQDEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_001172478.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172478.2

ORF Insert Sequence:

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGGT CGACTTATCA 
AAGGATCTCC CTCACTGGAA CAAGCTTAAA GCAGATGAGA AGTACTTCAT CTCTCACATC 
TTAGCCTTTT TTGCAGCCAG TGATGGAATT GTAAATGAAA ATTTGGTGGA GCGCTTTAGT 
CAGGAGGTGC AGGTTCCAGA GGCTCGCTGT TTCTATGGCT TTCAAATTCT CATCGAGAAT 
GTTCACTCAG AGATGTACAG TTTGCTGATA GACACTTACA TCAGAGATCC CAAGAAAAGG 
GAATTTTTAT TTAATGCAAT TGAAACCATG CCCTATGTTA AGAAAAAAGC AGATTGGGCC 
TTGCGATGGA TAGCAGATAG AAAATCTACT TTTGGGGAAA GAGTGGTGGC CTTTGCTGCT 
GTAGAAGGAG TTTTCTTCTC AGGATCTTTT GCTGCTATAT TCTGGCTAAA GAAGAGAGGT 
CTTATGCCAG GACTCACTTT TTCCAATGAA CTCATCAGCA GAGATGAAGG ACTTCACTGT 
GACTTTGCTT GCCTGATGTT CCAATACTTA GTAAATAAGC CTTCAGAAGA AAGGGTCAGG 
GAGATCATTG TTGATGCTGT CAAAATTGAG CAGGAGTTTT TAACAGAAGC CTTGCCAGTT 
GGCCTCATTG GAATGAATTG CATTTTGATG AAACAGTACA TTGAGTTTGT AGCTGACAGA 
TTACTTGTGG AACTTGGATT CTCAAAGGTT TTTCAGGCAG AAAATCCTTT TGATTTTATG 
GAAAACATTT CTTTAGAAGG AAAAACAAAT TTCTTTGAGA AACGAGTTTC AGAGTATCAG 
CGTTTTGCAG TTATGGCAGA AACCACAGAT AACGTCTTCA CCTTGGATGC AGATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRM2B ( NM_001172478.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165949.1 Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26172
Clone ID Related Accession (Same CDS sequence)	NM_001172478.2 , NM_001172478.1
Accession Version	NM_001172478.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-11
Organism	Homo sapiens(human)
Product	ribonucleoside-diphosphate reductase subunit M2 B isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB036063.1, BC108261.1 and AK023605.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. This results in a shorter protein (isoform 3), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.214736.1, SRR1803611.31847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGGT CGACTTATCA 
AAGGATCTCC CTCACTGGAA CAAGCTTAAA GCAGATGAGA AGTACTTCAT CTCTCACATC 
TTAGCCTTTT TTGCAGCCAG TGATGGAATT GTAAATGAAA ATTTGGTGGA GCGCTTTAGT 
CAGGAGGTGC AGGTTCCAGA GGCTCGCTGT TTCTATGGCT TTCAAATTCT CATCGAGAAT 
GTTCACTCAG AGATGTACAG TTTGCTGATA GACACTTACA TCAGAGATCC CAAGAAAAGG 
GAATTTTTAT TTAATGCAAT TGAAACCATG CCCTATGTTA AGAAAAAAGC AGATTGGGCC 
TTGCGATGGA TAGCAGATAG AAAATCTACT TTTGGGGAAA GAGTGGTGGC CTTTGCTGCT 
GTAGAAGGAG TTTTCTTCTC AGGATCTTTT GCTGCTATAT TCTGGCTAAA GAAGAGAGGT 
CTTATGCCAG GACTCACTTT TTCCAATGAA CTCATCAGCA GAGATGAAGG ACTTCACTGT 
GACTTTGCTT GCCTGATGTT CCAATACTTA GTAAATAAGC CTTCAGAAGA AAGGGTCAGG 
GAGATCATTG TTGATGCTGT CAAAATTGAG CAGGAGTTTT TAACAGAAGC CTTGCCAGTT 
GGCCTCATTG GAATGAATTG CATTTTGATG AAACAGTACA TTGAGTTTGT AGCTGACAGA 
TTACTTGTGG AACTTGGATT CTCAAAGGTT TTTCAGGCAG AAAATCCTTT TGATTTTATG 
GAAAACATTT CTTTAGAAGG AAAAACAAAT TTCTTTGAGA AACGAGTTTC AGAGTATCAG 
CGTTTTGCAG TTATGGCAGA AACCACAGAT AACGTCTTCA CCTTGGATGC AGATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165949.1
CDS	245..1144
Translation	MGDPERPEAAGLDQDEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_001172478.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172478.1

ORF Insert Sequence:

ATGGGCGACC CGGAAAGGCC GGAAGCGGCC GGGCTGGATC AGGATGAGGT CGACTTATCA 
AAGGATCTCC CTCACTGGAA CAAGCTTAAA GCAGATGAGA AGTACTTCAT CTCTCACATC 
TTAGCCTTTT TTGCAGCCAG TGATGGAATT GTAAATGAAA ATTTGGTGGA GCGCTTTAGT 
CAGGAGGTGC AGGTTCCAGA GGCTCGCTGT TTCTATGGCT TTCAAATTCT CATCGAGAAT 
GTTCACTCAG AGATGTACAG TTTGCTGATA GACACTTACA TCAGAGATCC CAAGAAAAGG 
GAATTTTTAT TTAATGCAAT TGAAACCATG CCCTATGTTA AGAAAAAAGC AGATTGGGCC 
TTGCGATGGA TAGCAGATAG AAAATCTACT TTTGGGGAAA GAGTGGTGGC CTTTGCTGCT 
GTAGAAGGAG TTTTCTTCTC AGGATCTTTT GCTGCTATAT TCTGGCTAAA GAAGAGAGGT 
CTTATGCCAG GACTCACTTT TTCCAATGAA CTCATCAGCA GAGATGAAGG ACTTCACTGT 
GACTTTGCTT GCCTGATGTT CCAATACTTA GTAAATAAGC CTTCAGAAGA AAGGGTCAGG 
GAGATCATTG TTGATGCTGT CAAAATTGAG CAGGAGTTTT TAACAGAAGC CTTGCCAGTT 
GGCCTCATTG GAATGAATTG CATTTTGATG AAACAGTACA TTGAGTTTGT AGCTGACAGA 
TTACTTGTGG AACTTGGATT CTCAAAGGTT TTTCAGGCAG AAAATCCTTT TGATTTTATG 
GAAAACATTT CTTTAGAAGG AAAAACAAAT TTCTTTGAGA AACGAGTTTC AGAGTATCAG 
CGTTTTGCAG TTATGGCAGA AACCACAGAT AACGTCTTCA CCTTGGATGC AGATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RRM2B ( NM_001172477.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165948.1 Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 2, mRNA.

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CloneID	OHu26188
Clone ID Related Accession (Same CDS sequence)	NM_001172477.1
Accession Version	NM_001172477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	ribonucleoside-diphosphate reductase subunit M2 B isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC308409.1, AK304354.1, BC108261.1 and AK023605.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.142490.1, SRR1660807.245670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTGCTGT TGCGTCTTCC CCCTCACCGC AGTCACGCCA GCCCGTTAGA TTGCAAGTTG 
CAGGACCGCT GTAGGAAATG TTATTCGCCG CGGTCAGGAC AGGCCTGTCC GCCCGCCCTC 
GCCGCAGCCT GGCTTCGTCG TTGCGAGCGC CGGGGAGGCC GTCCCCGGGG AGGGCGGAGG 
AAGGAGCTGA CTTTGGGTTT GCGTCCCGCT CGCTGCTCTG CCCCGGGGCC AGCCAAGGAC 
GACGCTTGGA GGCCTCAGGC CGGGAGATCA TCTTCAGACA CCAACGAAAG TGAAATAAAG 
TCAAATGAAG AGCCACTCCT AAGAAAGAGT TCTCGCCGGT TTGTCATCTT TCCAATCCAG 
TACCCTGATA TTTGGAAAAT GTATAAACAG GCACAGGCTT CCTTCTGGAC AGCAGAAGAG 
GTCGACTTAT CAAAGGATCT CCCTCACTGG AACAAGCTTA AAGCAGATGA GAAGTACTTC 
ATCTCTCACA TCTTAGCCTT TTTTGCAGCC AGTGATGGAA TTGTAAATGA AAATTTGGTG 
GAGCGCTTTA GTCAGGAGGT GCAGGTTCCA GAGGCTCGCT GTTTCTATGG CTTTCAAATT 
CTCATCGAGA ATGTTCACTC AGAGATGTAC AGTTTGCTGA TAGACACTTA CATCAGAGAT 
CCCAAGAAAA GGGAATTTTT ATTTAATGCA ATTGAAACCA TGCCCTATGT TAAGAAAAAA 
GCAGATTGGG CCTTGCGATG GATAGCAGAT AGAAAATCTA CTTTTGGGGA AAGAGTGGTG 
GCCTTTGCTG CTGTAGAAGG AGTTTTCTTC TCAGGATCTT TTGCTGCTAT ATTCTGGCTA 
AAGAAGAGAG GTCTTATGCC AGGACTCACT TTTTCCAATG AACTCATCAG CAGAGATGAA 
GGACTTCACT GTGACTTTGC TTGCCTGATG TTCCAATACT TAGTAAATAA GCCTTCAGAA 
GAAAGGGTCA GGGAGATCAT TGTTGATGCT GTCAAAATTG AGCAGGAGTT TTTAACAGAA 
GCCTTGCCAG TTGGCCTCAT TGGAATGAAT TGCATTTTGA TGAAACAGTA CATTGAGTTT 
GTAGCTGACA GATTACTTGT GGAACTTGGA TTCTCAAAGG TTTTTCAGGC AGAAAATCCT 
TTTGATTTTA TGGAAAACAT TTCTTTAGAA GGAAAAACAA ATTTCTTTGA GAAACGAGTT 
TCAGAGTATC AGCGTTTTGC AGTTATGGCA GAAACCACAG ATAACGTCTT CACCTTGGAT 
GCAGATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165948.1
CDS	10..1281
Translation	MLLLRLPPHRSHASPLDCKLQDRCRKCYSPRSGQACPPALAAAWLRRCERRGGRPRGGRRKELTLGLRPARCSAPGPAKDDAWRPQAGRSSSDTNESEIKSNEEPLLRKSSRRFVIFPIQYPDIWKMYKQAQASFWTAEEVDLSKDLPHWNKLKADEKYFISHILAFFAASDGIVNENLVERFSQEVQVPEARCFYGFQILIENVHSEMYSLLIDTYIRDPKKREFLFNAIETMPYVKKKADWALRWIADRKSTFGERVVAFAAVEGVFFSGSFAAIFWLKKRGLMPGLTFSNELISRDEGLHCDFACLMFQYLVNKPSEERVREIIVDAVKIEQEFLTEALPVGLIGMNCILMKQYIEFVADRLLVELGFSKVFQAENPFDFMENISLEGKTNFFEKRVSEYQRFAVMAETTDNVFTLDADF

Target ORF information:

RefSeq Version	NM_001172477.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribonucleotide reductase regulatory TP53 inducible subunit M2B (RRM2B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172477.1

ORF Insert Sequence:

ATGTTGCTGT TGCGTCTTCC CCCTCACCGC AGTCACGCCA GCCCGTTAGA TTGCAAGTTG 
CAGGACCGCT GTAGGAAATG TTATTCGCCG CGGTCAGGAC AGGCCTGTCC GCCCGCCCTC 
GCCGCAGCCT GGCTTCGTCG TTGCGAGCGC CGGGGAGGCC GTCCCCGGGG AGGGCGGAGG 
AAGGAGCTGA CTTTGGGTTT GCGTCCCGCT CGCTGCTCTG CCCCGGGGCC AGCCAAGGAC 
GACGCTTGGA GGCCTCAGGC CGGGAGATCA TCTTCAGACA CCAACGAAAG TGAAATAAAG 
TCAAATGAAG AGCCACTCCT AAGAAAGAGT TCTCGCCGGT TTGTCATCTT TCCAATCCAG 
TACCCTGATA TTTGGAAAAT GTATAAACAG GCACAGGCTT CCTTCTGGAC AGCAGAAGAG 
GTCGACTTAT CAAAGGATCT CCCTCACTGG AACAAGCTTA AAGCAGATGA GAAGTACTTC 
ATCTCTCACA TCTTAGCCTT TTTTGCAGCC AGTGATGGAA TTGTAAATGA AAATTTGGTG 
GAGCGCTTTA GTCAGGAGGT GCAGGTTCCA GAGGCTCGCT GTTTCTATGG CTTTCAAATT 
CTCATCGAGA ATGTTCACTC AGAGATGTAC AGTTTGCTGA TAGACACTTA CATCAGAGAT 
CCCAAGAAAA GGGAATTTTT ATTTAATGCA ATTGAAACCA TGCCCTATGT TAAGAAAAAA 
GCAGATTGGG CCTTGCGATG GATAGCAGAT AGAAAATCTA CTTTTGGGGA AAGAGTGGTG 
GCCTTTGCTG CTGTAGAAGG AGTTTTCTTC TCAGGATCTT TTGCTGCTAT ATTCTGGCTA 
AAGAAGAGAG GTCTTATGCC AGGACTCACT TTTTCCAATG AACTCATCAG CAGAGATGAA 
GGACTTCACT GTGACTTTGC TTGCCTGATG TTCCAATACT TAGTAAATAA GCCTTCAGAA 
GAAAGGGTCA GGGAGATCAT TGTTGATGCT GTCAAAATTG AGCAGGAGTT TTTAACAGAA 
GCCTTGCCAG TTGGCCTCAT TGGAATGAAT TGCATTTTGA TGAAACAGTA CATTGAGTTT 
GTAGCTGACA GATTACTTGT GGAACTTGGA TTCTCAAAGG TTTTTCAGGC AGAAAATCCT 
TTTGATTTTA TGGAAAACAT TTCTTTAGAA GGAAAAACAA ATTTCTTTGA GAAACGAGTT 
TCAGAGTATC AGCGTTTTGC AGTTATGGCA GAAACCACAG ATAACGTCTT CACCTTGGAT 
GCAGATTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Overexpression of p53R2 is associated with poor prognosis in lung sarcomatoid carcinoma.
BMC cancer17(1)855(2017 Dec)
Chen J,Xiao Y,Cai X,Liu J,Chen K,Zhang X

p53R2 as a novel prognostic biomarker in nasopharyngeal carcinoma.
BMC cancer17(1)846(2017 Dec)
Chen J,Li S,Xiao Y,Zou X,Zhang X,Zhu M,Cai M,Xie D

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RRM2B cDNA ORF clone, Homo sapiens(human)

Gene

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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