NDUFB10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFB10
Entrez Gene ID	4716
Full Name	NADH:ubiquinone oxidoreductase subunit B10
Synonyms	PDSW
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit B10 Names NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 CI-PDSW NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3) NADH-ubiquinone oxidoreductase PDSW subunit complex I PDSW subunit complex I-PDSW
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Disorder MIM:	603843

mRNA and Protein(s)

mRNA	Protein	Name
NM_004548.3	NP_004539.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
NM_004548.2	NP_004539.1	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Pdsw	NP_722908.1
Bos taurus (cattle)	NDUFB10	NP_787012.1
Gallus gallus (chicken)	NDUFB10	XP_414844.1
Danio rerio (zebrafish)	ndufb10	NP_957024.1
Rattus norvegicus (Norway rat)	Ndufb10	NP_001102913.1
Caenorhabditis elegans (roundworm)	CELE_F59C6.5	NP_492752.1
Homo sapiens (human)	NDUFB10	NP_004539.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007780	XP_317733.3
Pan troglodytes (chimpanzee)	NDUFB10	NP_001065292.1
Canis lupus familiaris (dog)	NDUFB10	XP_537012.1
Mus musculus (house mouse)	Ndufb10	NP_080960.1
Xenopus tropicalis (tropical clawed frog)	ndufb10	NP_001017250.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly.
Friederich MW, Erdogan AJ, Coughlin CR 2nd, Elos MT, Jiang H, O'Rourke CP, Lovell MA, Wartchow E, Gowan K, Chatfield KC, Chick WS, Spector EB, Van Hove JLK, Riemer J
Human molecular genetics26(4)702-716(2017 Feb)

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Stroud DA, Surgenor EE, Formosa LE, Reljic B, Frazier AE, Dibley MG, Osellame LD, Stait T, Beilharz TH, Thorburn DR, Salim A, Ryan MT
Nature538(7623)123-126(2016 Oct)

Preservation of NADH ubiquinone-oxidoreductase activity by Src kinase-mediated phosphorylation of NDUFB10.
Hebert-Chatelain E, Jose C, Gutierrez Cortes N, Dupuy JW, Rocher C, Dachary-Prigent J, Letellier T
Biochimica et biophysica acta1817(5)718-25(2012 May)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey.
Starr JM, Shiels PG, Harris SE, Pattie A, Pearce MS, Relton CL, Deary IJ
Mechanisms of ageing and development129(12)745-51(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings indicate that mutations in NDUFB10 are a novel cause of complex I deficiency associated with a late stage assembly defect and emphasize the role of intermembrane space proteins for the efficient assembly of complex I.
Title: Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly.

Src kinase inhibition led to a decrease in mitochondrial respiration via a specific decrease in complex I activities which is associated with a lower phosphorylation of the complex I subunit NDUFB10.
Title: Preservation of NADH ubiquinone-oxidoreductase activity by Src kinase-mediated phosphorylation of NDUFB10.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey.

Observational study of gene-disease association. (HuGE Navigator)
Title: A genetic association analysis of cognitive ability and cognitive ageing using 325 markers for 109 genes associated with oxidative stress or cognition.

The following NDUFB10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFB10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu31992	NM_004548.3 Latest version!	Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu31992	NM_004548.2	Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFB10 ( NM_004548.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004539.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.

Price & Availability↑

CloneID	OHu31992
Clone ID Related Accession (Same CDS sequence)	NM_004548.3 , NM_004548.2
Accession Version	NM_004548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP319067.1 and BC005829.2. On Nov 22, 2018 this sequence version replaced NM_004548.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF088991.1, SRR1163655.207088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000268668.11/ ENSP00000268668.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGACA GCTGGGACAA GGATGTGTAC CCTGAGCCCC CGCGCCGCAC GCCGGTGCAG 
CCCAATCCCA TCGTCTACAT GATGAAAGCG TTCGACCTCA TCGTGGACCG ACCCGTGACC 
CTCGTGAGAG AATTTATAGA GCGGCAGCAC GCAAAGAACA GGTATTACTA CTACCACCGG 
CAGTACCGCC GCGTGCCAGA CATCACTGAG TGCAAGGAGG AGGACATCAT GTGCATGTAT 
GAAGCCGAAA TGCAGTGGAA GAGGGACTAC AAAGTCGACC AAGAAATTAT CAACATTATG 
CAGGATCGGC TCAAAGCCTG TCAGCAGAGG GAAGGACAGA ACTACCAGCA GAACTGTATC 
AAGGAAGTGG AGCAGTTCAC CCAGGTGGCC AAGGCCTACC AGGACCGCTA TCAGGACCTG 
GGGGCCTACA GTTCTGCCAG GAAGTGCCTG GCCAAACAGA GGCAGAGGAT GCTGCAAGAG 
AGAAAAGCTG CAAAAGAGGC CGCCGCTGCC ACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004539.1
CDS	88..606
Translation	MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS

Target ORF information:

RefSeq Version	NM_004548.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004548.3

ORF Insert Sequence:

ATGCCGGACA GCTGGGACAA GGATGTGTAC CCTGAGCCCC CGCGCCGCAC GCCGGTGCAG 
CCCAATCCCA TCGTCTACAT GATGAAAGCG TTCGACCTCA TCGTGGACCG ACCCGTGACC 
CTCGTGAGAG AATTTATAGA GCGGCAGCAC GCAAAGAACA GGTATTACTA CTACCACCGG 
CAGTACCGCC GCGTGCCAGA CATCACTGAG TGCAAGGAGG AGGACATCAT GTGCATGTAT 
GAAGCCGAAA TGCAGTGGAA GAGGGACTAC AAAGTCGACC AAGAAATTAT CAACATTATG 
CAGGATCGGC TCAAAGCCTG TCAGCAGAGG GAAGGACAGA ACTACCAGCA GAACTGTATC 
AAGGAAGTGG AGCAGTTCAC CCAGGTGGCC AAGGCCTACC AGGACCGCTA TCAGGACCTG 
GGGGCCTACA GTTCTGCCAG GAAGTGCCTG GCCAAACAGA GGCAGAGGAT GCTGCAAGAG 
AGAAAAGCTG CAAAAGAGGC CGCCGCTGCC ACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFB10 ( NM_004548.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004539.1 Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.

Price & Availability↑

CloneID	OHu31992
Clone ID Related Accession (Same CDS sequence)	NM_004548.3 , NM_004548.2
Accession Version	NM_004548.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP319067.1 and BC005829.2. On Nov 28, 2007 this sequence version replaced NM_004548.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF088991.1, ERR279842.3520.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCGGACA GCTGGGACAA GGATGTGTAC CCTGAGCCCC CGCGCCGCAC GCCGGTGCAG 
CCCAATCCCA TCGTCTACAT GATGAAAGCG TTCGACCTCA TCGTGGACCG ACCCGTGACC 
CTCGTGAGAG AATTTATAGA GCGGCAGCAC GCAAAGAACA GGTATTACTA CTACCACCGG 
CAGTACCGCC GCGTGCCAGA CATCACTGAG TGCAAGGAGG AGGACATCAT GTGCATGTAT 
GAAGCCGAAA TGCAGTGGAA GAGGGACTAC AAAGTCGACC AAGAAATTAT CAACATTATG 
CAGGATCGGC TCAAAGCCTG TCAGCAGAGG GAAGGACAGA ACTACCAGCA GAACTGTATC 
AAGGAAGTGG AGCAGTTCAC CCAGGTGGCC AAGGCCTACC AGGACCGCTA TCAGGACCTG 
GGGGCCTACA GTTCTGCCAG GAAGTGCCTG GCCAAACAGA GGCAGAGGAT GCTGCAAGAG 
AGAAAAGCTG CAAAAGAGGC CGCCGCTGCC ACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004539.1
CDS	110..628
Translation	MPDSWDKDVYPEPPRRTPVQPNPIVYMMKAFDLIVDRPVTLVREFIERQHAKNRYYYYHRQYRRVPDITECKEEDIMCMYEAEMQWKRDYKVDQEIINIMQDRLKACQQREGQNYQQNCIKEVEQFTQVAKAYQDRYQDLGAYSSARKCLAKQRQRMLQERKAAKEAAAATS

Target ORF information:

RefSeq Version	NM_004548.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NADH:ubiquinone oxidoreductase subunit B10 (NDUFB10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004548.2

ORF Insert Sequence:

ATGCCGGACA GCTGGGACAA GGATGTGTAC CCTGAGCCCC CGCGCCGCAC GCCGGTGCAG 
CCCAATCCCA TCGTCTACAT GATGAAAGCG TTCGACCTCA TCGTGGACCG ACCCGTGACC 
CTCGTGAGAG AATTTATAGA GCGGCAGCAC GCAAAGAACA GGTATTACTA CTACCACCGG 
CAGTACCGCC GCGTGCCAGA CATCACTGAG TGCAAGGAGG AGGACATCAT GTGCATGTAT 
GAAGCCGAAA TGCAGTGGAA GAGGGACTAC AAAGTCGACC AAGAAATTAT CAACATTATG 
CAGGATCGGC TCAAAGCCTG TCAGCAGAGG GAAGGACAGA ACTACCAGCA GAACTGTATC 
AAGGAAGTGG AGCAGTTCAC CCAGGTGGCC AAGGCCTACC AGGACCGCTA TCAGGACCTG 
GGGGCCTACA GTTCTGCCAG GAAGTGCCTG GCCAAACAGA GGCAGAGGAT GCTGCAAGAG 
AGAAAAGCTG CAAAAGAGGC CGCCGCTGCC ACCTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in the accessory subunit NDUFB10 result in isolated complex I deficiency and illustrate the critical role of intermembrane space import for complex I holoenzyme assembly.
Human molecular genetics26(4)702-716(2017 Feb)
Friederich MW,Erdogan AJ,Coughlin CR 2nd,Elos MT,Jiang H,O'Rourke CP,Lovell MA,Wartchow E,Gowan K,Chatfield KC,Chick WS,Spector EB,Van Hove JLK,Riemer J

Accessory subunits are integral for assembly and function of human mitochondrial complex I.
Nature538(7623)123-126(2016 Oct)
Stroud DA,Surgenor EE,Formosa LE,Reljic B,Frazier AE,Dibley MG,Osellame LD,Stait T,Beilharz TH,Thorburn DR,Salim A,Ryan MT

Preservation of NADH ubiquinone-oxidoreductase activity by Src kinase-mediated phosphorylation of NDUFB10.
Biochimica et biophysica acta1817(5)718-25(2012 May)
Hebert-Chatelain E,Jose C,Gutierrez Cortes N,Dupuy JW,Rocher C,Dachary-Prigent J,Letellier T

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

Oxidative stress, telomere length and biomarkers of physical aging in a cohort aged 79 years from the 1932 Scottish Mental Survey.
Mechanisms of ageing and development129(12)745-51(2008 Dec)
Starr JM,Shiels PG,Harris SE,Pattie A,Pearce MS,Relton CL,Deary IJ