ORF » Species Summary » Homo sapiens » PRAMEF25 cDNA ORF clone

PRAMEF25 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following PRAMEF25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRAMEF25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu34026 NM_001310134.1
Latest version!		 Homo sapiens PRAME family member 25 (PRAMEF25), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$159.50-$223.30
$319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PRAMEF25 ( NM_001310134.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001297063.1 Homo sapiens PRAME family member 25 (PRAMEF25), mRNA.
    Price & Availability↑
    CloneID OHu34026
    Clone ID Related Accession (Same CDS sequence) NM_001310134.1
    Accession Version NM_001310134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1437bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Homo sapiens(human)
    Product PRAME family member 25
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AC245056.3. On or before Jun 30, 2015 this sequence version replaced XM_006711082.2, XM_005276123.2. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by paralogous transcripts. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2158188, SAMN03465410 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGATGA GCATCCGGAC TCCACCCAGA CTCCTGGAGC TGGCGGGGCG GAGCGTGCTG 
    AGGGACCAAG CCTTGGCCAT GTCCACCCTG GAGGAGCTGC CCACAGAACT TTTCCCCCCA 
    CTGTTCATGG AGGCCTTCAG CAGGAGACGC TGTGAGGCCC TGAAGCTGAT GGTACAGGCC 
    TGGCCCTTCC GCCGCCTCCC TCTGAGGCCT CTGATAAAGA TGCCTTGTCT GGAGACCTTC 
    CAAGCTGTGC TCAATGGGCT TGATGCACTG CTTACCCACG GGGTTCGTCC CAGGAGGTGG 
    AAACTTCAAG TGCTGGATTT ACAGGATGTC TGTGAGAACT TCTGGATGGT TTGGTCTGAA 
    GCTATGGCCC GTGGGTGCTT CCTCAATGCC AAGAGGAACA AAAAACCAGT GCAGGACTGT 
    CCAAGGATGA GAGGACGGCA GCCCTTGACT GTGTTCGTAG AACTTTGGCT CAAGAACAGG 
    ACTCTGGATG AACACCTCAC CTGCCTCCTT CTATGGGTCA AGCAGAGGAA AGATTTACTA 
    CACCTGTGCT GTAAGAAGCT GAAAATTTTG GGAATGCCCT TCCGCAATAT CAGAAGCATC 
    CTGAAAATGG TGAACCTAGA CTGTATCCAG GAGGTGGAAG TGAATTGCAA GTGGGTACTG 
    CCCATCCTGA CACAGTTTAC CCCATACCTG GGCCACATGA GGAATCTTCA GAAGCTCGTT 
    CTCTCCCACA TGGATGTCTC TCGCTACGTT TCCCCAGAGC AGAAGAAGGA GATTGTTACC 
    CAGTTCACCA CTCAGTTCCT CAAGCTGCAC TGCCTCCAAA AGCTTTATAT GAACTCTGTT 
    TCTTTCCTCG AAGGCCACCT GGACCAGCTG CTCAGCTGTC TGAAGACCTC GTTAAAGGTC 
    CTCACAATAA CTAACTGTGT GCTTTTGGAA TCAGACTTGA AGCATCTATC CCAGTGCCCG 
    AGTATCAGTC AACTAAAGAC CCTGGACCTG AGTGGCATCA GACTGACCAA TTACAGTCTT 
    GTGCCTCTCC AAATTCTCCT AGAAAAAGTT GCAGCCACCC TTGAGTACCT GGATTTAGAT 
    GACTGTGGCA TCATAGACTC CCAAGTCAAC GCCATCCTGC CTGCCCTGAG CCGCTGCTTT 
    GAGCTCAACA CCTTCAGCTT CTGTGGAAAT CCCATCTCCA TGGCCACCCT GGAGAACCTG 
    CTGAGCCACA CAATCATACT CAAAAACTTA TGCCTGGAGC TGTATCCTGC CCCACGGGAG 
    AGTTATGGTG CTGATGGTAC TCTCTGCTGG AGCAGATTTA CTCAGATTAG GGCTGAGCTG 
    ATGAAGAGAG TTAGGGACTT AAGGCACCCC AAGAGGATCT TGTTCGGTAC TGACTACTGC 
    CCTGACTGTG GCAACAGGTC ATTTTATGAC CTGGAGGCAG ATCAATACTG CTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001297063.1
    CDS17..1453
    Translation

    Target ORF information:

    RefSeq Version NM_001310134.1
    Organism Homo sapiens(human)
    Definition Homo sapiens PRAME family member 25 (PRAMEF25), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001310134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGAAGATGA GCATCCGGAC TCCACCCAGA CTCCTGGAGC TGGCGGGGCG GAGCGTGCTG 
    AGGGACCAAG CCTTGGCCAT GTCCACCCTG GAGGAGCTGC CCACAGAACT TTTCCCCCCA 
    CTGTTCATGG AGGCCTTCAG CAGGAGACGC TGTGAGGCCC TGAAGCTGAT GGTACAGGCC 
    TGGCCCTTCC GCCGCCTCCC TCTGAGGCCT CTGATAAAGA TGCCTTGTCT GGAGACCTTC 
    CAAGCTGTGC TCAATGGGCT TGATGCACTG CTTACCCACG GGGTTCGTCC CAGGAGGTGG 
    AAACTTCAAG TGCTGGATTT ACAGGATGTC TGTGAGAACT TCTGGATGGT TTGGTCTGAA 
    GCTATGGCCC GTGGGTGCTT CCTCAATGCC AAGAGGAACA AAAAACCAGT GCAGGACTGT 
    CCAAGGATGA GAGGACGGCA GCCCTTGACT GTGTTCGTAG AACTTTGGCT CAAGAACAGG 
    ACTCTGGATG AACACCTCAC CTGCCTCCTT CTATGGGTCA AGCAGAGGAA AGATTTACTA 
    CACCTGTGCT GTAAGAAGCT GAAAATTTTG GGAATGCCCT TCCGCAATAT CAGAAGCATC 
    CTGAAAATGG TGAACCTAGA CTGTATCCAG GAGGTGGAAG TGAATTGCAA GTGGGTACTG 
    CCCATCCTGA CACAGTTTAC CCCATACCTG GGCCACATGA GGAATCTTCA GAAGCTCGTT 
    CTCTCCCACA TGGATGTCTC TCGCTACGTT TCCCCAGAGC AGAAGAAGGA GATTGTTACC 
    CAGTTCACCA CTCAGTTCCT CAAGCTGCAC TGCCTCCAAA AGCTTTATAT GAACTCTGTT 
    TCTTTCCTCG AAGGCCACCT GGACCAGCTG CTCAGCTGTC TGAAGACCTC GTTAAAGGTC 
    CTCACAATAA CTAACTGTGT GCTTTTGGAA TCAGACTTGA AGCATCTATC CCAGTGCCCG 
    AGTATCAGTC AACTAAAGAC CCTGGACCTG AGTGGCATCA GACTGACCAA TTACAGTCTT 
    GTGCCTCTCC AAATTCTCCT AGAAAAAGTT GCAGCCACCC TTGAGTACCT GGATTTAGAT 
    GACTGTGGCA TCATAGACTC CCAAGTCAAC GCCATCCTGC CTGCCCTGAG CCGCTGCTTT 
    GAGCTCAACA CCTTCAGCTT CTGTGGAAAT CCCATCTCCA TGGCCACCCT GGAGAACCTG 
    CTGAGCCACA CAATCATACT CAAAAACTTA TGCCTGGAGC TGTATCCTGC CCCACGGGAG 
    AGTTATGGTG CTGATGGTAC TCTCTGCTGG AGCAGATTTA CTCAGATTAG GGCTGAGCTG 
    ATGAAGAGAG TTAGGGACTT AAGGCACCCC AAGAGGATCT TGTTCGGTAC TGACTACTGC 
    CCTGACTGTG GCAACAGGTC ATTTTATGAC CTGGAGGCAG ATCAATACTG CTGTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
    Briefings in bioinformatics12(5)449-62(2011 Sep)
    Gaudet P,Livstone MS,Lewis SE,Thomas PD


    The DNA sequence and biological annotation of human chromosome 1.
    Nature441(7091)315-21(2006 May)
    Gregory SG,Barlow KF,McLay KE,Kaul R,Swarbreck D,Dunham A,Scott CE,Howe KL,Woodfine K,Spencer CC,Jones MC,Gillson C,Searle S,Zhou Y,Kokocinski F,McDonald L,Evans R,Phillips K,Atkinson A,Cooper R,Jones C,Hall RE,Andrews TD,Lloyd C,Ainscough R,Almeida JP,Ambrose KD,Anderson F,Andrew RW,Ashwell RI,Aubin K,Babbage AK,Bagguley CL,Bailey J,Beasley H,Bethel G,Bird CP,Bray-Allen S,Brown JY,Brown AJ,Buckley D,Burton J,Bye J,Carder C,Chapman JC,Clark SY,Clarke G,Clee C,Cobley V,Collier RE,Corby N,Coville GJ,Davies J,Deadman R,Dunn M,Earthrowl M,Ellington AG,Errington H,Frankish A,Frankland J,French L,Garner P,Garnett J,Gay L,Ghori MR,Gibson R,Gilby LM,Gillett W,Glithero RJ,Grafham DV,Griffiths C,Griffiths-Jones S,Grocock R,Hammond S,Harrison ES,Hart E,Haugen E,Heath PD,Holmes S,Holt K,Howden PJ,Hunt AR,Hunt SE,Hunter G,Isherwood J,James R,Johnson C,Johnson D,Joy A,Kay M,Kershaw JK,Kibukawa M,Kimberley AM,King A,Knights AJ,Lad H,Laird G,Lawlor S,Leongamornlert DA,Lloyd DM,Loveland J,Lovell J,Lush MJ,Lyne R,Martin S,Mashreghi-Mohammadi M,Matthews L,Matthews NS,McLaren S,Milne S,Mistry S,Moore MJ,Nickerson T,O'Dell CN,Oliver K,Palmeiri A,Palmer SA,Parker A,Patel D,Pearce AV,Peck AI,Pelan S,Phelps K,Phillimore BJ,Plumb R,Rajan J,Raymond C,Rouse G,Saenphimmachak C,Sehra HK,Sheridan E,Shownkeen R,Sims S,Skuce CD,Smith M,Steward C,Subramanian S,Sycamore N,Tracey A,Tromans A,Van Helmond Z,Wall M,Wallis JM,White S,Whitehead SL,Wilkinson JE,Willey DL,Williams H,Wilming L,Wray PW,Wu Z,Coulson A,Vaudin M,Sulston JE,Durbin R,Hubbard T,Wooster R,Dunham I,Carter NP,McVean G,Ross MT,Harrow J,Olson MV,Beck S,Rogers J,Bentley DR,Banerjee R,Bryant SP,Burford DC,Burrill WD,Clegg SM,Dhami P,Dovey O,Faulkner LM,Gribble SM,Langford CF,Pandian RD,Porter KM,Prigmore E


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