AREG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AREG
Entrez Gene ID	374
Full Name	amphiregulin
Synonyms	AR,AREGB,CRDGF,SDGF
General protein information	Preferred Names amphiregulin Names amphiregulin amphiregulin B colorectum cell-derived growth factor schwannoma-derived growth factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q13.3
Summary	The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014].
Disorder MIM:	104640

mRNA and Protein(s)

mRNA	Protein	Name
NM_001657.3	NP_001648.1	amphiregulin preproprotein
NM_001657.4	NP_001648.1	amphiregulin preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	AREG	NP_001648.1
Pan troglodytes (chimpanzee)	AREG	XP_517224.2
Rattus norvegicus (Norway rat)	Areg	NP_058819.1
Mus musculus (house mouse)	Areg	NP_033834.1
Gallus gallus (chicken)	AREGB	NP_001026708.1
Bos taurus (cattle)	LOC538751	NP_001092562.1
	AREGB	XP_001101978.1
Canis lupus familiaris (dog)	LOC475176	XP_005628351.1
Xenopus tropicalis (tropical clawed frog)	LOC100145251	NP_001120205.1

Gene Ontology
Bibliography

Related articles in PubMed

Amphiregulin as a Novel Resistance Factor for Amrubicin in Lung Cancer Cells.
Tokunaga S, Nagano T, Kobayashi K, Katsurada M, Nakata K, Yamamoto M, Tachihara M, Kamiryo H, Yokozaki H, Nishimura Y
Anticancer research37(5)2225-2231(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Amphiregulin plays an important role in lung neoplasm resistance to amrubicinol
Title: Amphiregulin as a Novel Resistance Factor for Amrubicin in Lung Cancer Cells.

EGF-AREG interplay in airway basal cell stem/progenitor cells is one of the mechanisms that mediates the interconnected pathogenesis of all major smoking-induced lesions in the human airway epithelium.
Title: EGF-Amphiregulin Interplay in Airway Stem/Progenitor Cells Links the Pathogenesis of Smoking-Induced Lesions in the Human Airway Epithelium.

blocking soluble amphiregulin with a neutralizing antibody also significantly increased apoptotic cell death of HepG2 cells due to treatment with methyl methanesulfonate, cisplatin, or a recombinant p53 adenovirus, suggesting that the function of amphiregulin involved in inhibiting apoptosis may be a common mechanism by which hepatoma cells escape from stimulus-induced apoptosis
Title: Amphiregulin impairs apoptosis-stimulating protein 2 of p53 overexpression-induced apoptosis in hepatoma cells.

Amphiregulin enhances VEGF-A production in human chondrosarcoma cells and promotes angiogenesis by inhibiting miR-206 via FAK/c-Src/PKCdelta pathway.
Title: Amphiregulin enhances VEGF-A production in human chondrosarcoma cells and promotes angiogenesis by inhibiting miR-206 via FAK/c-Src/PKC? pathway.

secretion of IL-13 and amphiregulin suggests Intrahepatic Innate lymphoid cells may be recruited to promote resolution and repair and thereby they may contribute to ongoing fibrogenesis in liver disease.
Title: Human intrahepatic ILC2 are IL-13positive amphiregulinpositive and their frequency correlates with model of end stage liver disease score.

The following AREG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AREG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24151	NM_001657.4 Latest version!	Homo sapiens amphiregulin (AREG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu24151	NM_001657.3	Homo sapiens amphiregulin (AREG), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AREG ( NM_001657.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001648.1 Homo sapiens amphiregulin (AREG), mRNA.

Price & Availability↑

CloneID	OHu24151
Clone ID Related Accession (Same CDS sequence)	NM_001657.3 , NM_001657.4
Accession Version	NM_001657.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	amphiregulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY047499.1, BC009799.1 and AW014754.1. On Apr 11, 2014 this sequence version replaced NM_001657.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009799.1, M30704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGAGCCC CGCTGCTACC GCCGGCGCCG GTGGTGCTGT CGCTCTTGAT ACTCGGCTCA 
GGCCATTATG CTGCTGGATT GGACCTCAAT GACACCTACT CTGGGAAGCG TGAACCATTT 
TCTGGGGACC ACAGTGCTGA TGGATTTGAG GTTACCTCAA GAAGTGAGAT GTCTTCAGGG 
AGTGAGATTT CCCCTGTGAG TGAAATGCCT TCTAGTAGTG AACCGTCCTC GGGAGCCGAC 
TATGACTACT CAGAAGAGTA TGATAACGAA CCACAAATAC CTGGCTATAT TGTCGATGAT 
TCAGTCAGAG TTGAACAGGT AGTTAAGCCC CCCCAAAACA AGACGGAAAG TGAAAATACT 
TCAGATAAAC CCAAAAGAAA GAAAAAGGGA GGCAAAAATG GAAAAAATAG AAGAAACAGA 
AAGAAGAAAA ATCCATGTAA TGCAGAATTT CAAAATTTCT GCATTCACGG AGAATGCAAA 
TATATAGAGC ACCTGGAAGC AGTAACATGC AAATGTCAGC AAGAATATTT CGGTGAACGG 
TGTGGGGAAA AGTCCATGAA AACTCACAGC ATGATTGACA GTAGTTTATC AAAAATTGCA 
TTAGCAGCCA TAGCTGCCTT TATGTCTGCT GTGATCCTCA CAGCTGTTGC TGTTATTACA 
GTCCAGCTTA GAAGACAATA CGTCAGGAAA TATGAAGGAG AAGCTGAGGA ACGAAAGAAA 
CTTCGACAAG AGAATGGAAA TGTACATGCT ATAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001648.1
CDS	249..1007
Translation	MRAPLLPPAPVVLSLLILGSGHYAAGLDLNDTYSGKREPFSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDNEPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSKIALAAIAAFMSAVILTAVAVITVQLRRQYVRKYEGEAEERKKLRQENGNVHAIA

Target ORF information:

RefSeq Version	NM_001657.3
Organism	Homo sapiens(human)
Definition	Homo sapiens amphiregulin (AREG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001657.3

ORF Insert Sequence:

ATGAGAGCCC CGCTGCTACC GCCGGCGCCG GTGGTGCTGT CGCTCTTGAT ACTCGGCTCA 
GGCCATTATG CTGCTGGATT GGACCTCAAT GACACCTACT CTGGGAAGCG TGAACCATTT 
TCTGGGGACC ACAGTGCTGA TGGATTTGAG GTTACCTCAA GAAGTGAGAT GTCTTCAGGG 
AGTGAGATTT CCCCTGTGAG TGAAATGCCT TCTAGTAGTG AACCGTCCTC GGGAGCCGAC 
TATGACTACT CAGAAGAGTA TGATAACGAA CCACAAATAC CTGGCTATAT TGTCGATGAT 
TCAGTCAGAG TTGAACAGGT AGTTAAGCCC CCCCAAAACA AGACGGAAAG TGAAAATACT 
TCAGATAAAC CCAAAAGAAA GAAAAAGGGA GGCAAAAATG GAAAAAATAG AAGAAACAGA 
AAGAAGAAAA ATCCATGTAA TGCAGAATTT CAAAATTTCT GCATTCACGG AGAATGCAAA 
TATATAGAGC ACCTGGAAGC AGTAACATGC AAATGTCAGC AAGAATATTT CGGTGAACGG 
TGTGGGGAAA AGTCCATGAA AACTCACAGC ATGATTGACA GTAGTTTATC AAAAATTGCA 
TTAGCAGCCA TAGCTGCCTT TATGTCTGCT GTGATCCTCA CAGCTGTTGC TGTTATTACA 
GTCCAGCTTA GAAGACAATA CGTCAGGAAA TATGAAGGAG AAGCTGAGGA ACGAAAGAAA 
CTTCGACAAG AGAATGGAAA TGTACATGCT ATAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AREG ( NM_001657.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001648.1 Homo sapiens amphiregulin (AREG), mRNA.

Price & Availability↑

CloneID	OHu24151
Clone ID Related Accession (Same CDS sequence)	NM_001657.3 , NM_001657.4
Accession Version	NM_001657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	amphiregulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC009799.1 and AW014754.1. On Nov 22, 2018 this sequence version replaced NM_001657.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M30704.1, BC009799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000395748.8/ ENSP00000379097.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGAGCCC CGCTGCTACC GCCGGCGCCG GTGGTGCTGT CGCTCTTGAT ACTCGGCTCA 
GGCCATTATG CTGCTGGATT GGACCTCAAT GACACCTACT CTGGGAAGCG TGAACCATTT 
TCTGGGGACC ACAGTGCTGA TGGATTTGAG GTTACCTCAA GAAGTGAGAT GTCTTCAGGG 
AGTGAGATTT CCCCTGTGAG TGAAATGCCT TCTAGTAGTG AACCGTCCTC GGGAGCCGAC 
TATGACTACT CAGAAGAGTA TGATAACGAA CCACAAATAC CTGGCTATAT TGTCGATGAT 
TCAGTCAGAG TTGAACAGGT AGTTAAGCCC CCCCAAAACA AGACGGAAAG TGAAAATACT 
TCAGATAAAC CCAAAAGAAA GAAAAAGGGA GGCAAAAATG GAAAAAATAG AAGAAACAGA 
AAGAAGAAAA ATCCATGTAA TGCAGAATTT CAAAATTTCT GCATTCACGG AGAATGCAAA 
TATATAGAGC ACCTGGAAGC AGTAACATGC AAATGTCAGC AAGAATATTT CGGTGAACGG 
TGTGGGGAAA AGTCCATGAA AACTCACAGC ATGATTGACA GTAGTTTATC AAAAATTGCA 
TTAGCAGCCA TAGCTGCCTT TATGTCTGCT GTGATCCTCA CAGCTGTTGC TGTTATTACA 
GTCCAGCTTA GAAGACAATA CGTCAGGAAA TATGAAGGAG AAGCTGAGGA ACGAAAGAAA 
CTTCGACAAG AGAATGGAAA TGTACATGCT ATAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001648.1
CDS	211..969
Translation	MRAPLLPPAPVVLSLLILGSGHYAAGLDLNDTYSGKREPFSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDNEPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRNRKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERCGEKSMKTHSMIDSSLSKIALAAIAAFMSAVILTAVAVITVQLRRQYVRKYEGEAEERKKLRQENGNVHAIA

Target ORF information:

RefSeq Version	NM_001657.4
Organism	Homo sapiens(human)
Definition	Homo sapiens amphiregulin (AREG), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001657.4

ORF Insert Sequence:

ATGAGAGCCC CGCTGCTACC GCCGGCGCCG GTGGTGCTGT CGCTCTTGAT ACTCGGCTCA 
GGCCATTATG CTGCTGGATT GGACCTCAAT GACACCTACT CTGGGAAGCG TGAACCATTT 
TCTGGGGACC ACAGTGCTGA TGGATTTGAG GTTACCTCAA GAAGTGAGAT GTCTTCAGGG 
AGTGAGATTT CCCCTGTGAG TGAAATGCCT TCTAGTAGTG AACCGTCCTC GGGAGCCGAC 
TATGACTACT CAGAAGAGTA TGATAACGAA CCACAAATAC CTGGCTATAT TGTCGATGAT 
TCAGTCAGAG TTGAACAGGT AGTTAAGCCC CCCCAAAACA AGACGGAAAG TGAAAATACT 
TCAGATAAAC CCAAAAGAAA GAAAAAGGGA GGCAAAAATG GAAAAAATAG AAGAAACAGA 
AAGAAGAAAA ATCCATGTAA TGCAGAATTT CAAAATTTCT GCATTCACGG AGAATGCAAA 
TATATAGAGC ACCTGGAAGC AGTAACATGC AAATGTCAGC AAGAATATTT CGGTGAACGG 
TGTGGGGAAA AGTCCATGAA AACTCACAGC ATGATTGACA GTAGTTTATC AAAAATTGCA 
TTAGCAGCCA TAGCTGCCTT TATGTCTGCT GTGATCCTCA CAGCTGTTGC TGTTATTACA 
GTCCAGCTTA GAAGACAATA CGTCAGGAAA TATGAAGGAG AAGCTGAGGA ACGAAAGAAA 
CTTCGACAAG AGAATGGAAA TGTACATGCT ATAGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Amphiregulin as a Novel Resistance Factor for Amrubicin in Lung Cancer Cells.
Anticancer research37(5)2225-2231(2017 05)
Tokunaga S,Nagano T,Kobayashi K,Katsurada M,Nakata K,Yamamoto M,Tachihara M,Kamiryo H,Yokozaki H,Nishimura Y

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AREG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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