EYS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EYS
Entrez Gene ID	346007
Full Name	eyes shut homolog (Drosophila)
Synonyms	C6orf178,C6orf179,C6orf180,EGFL10,EGFL11,RP25,SPAM,bA166P24.2,bA307F22.3,bA74E24.1,dJ1018A4.2,dJ22I17.2,dJ303F19.1
General protein information	Preferred Names eyes shut homolog (Drosophila) Names protein eyes shut homolog EGF-like-domain, multiple 10 EGF-like-domain, multiple 11 epidermal growth factor-like protein 10 epidermal growth factor-like protein 11 protein spacemaker homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q12
Summary	The product of this gene contains multiple epidermal growth factor (EGF)-like and LamG domains. The protein is expressed in the photoreceptor layer of the retina, and the gene is mutated in autosomal recessive retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008].
Disorder MIM:	612424
Disorder Html:	Retinitis pigmentosa 25, 602772 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001142800.1	NP_001136272.1	protein eyes shut homolog isoform 1 precursor
NM_001142801.1	NP_001136273.1	protein eyes shut homolog isoform 2 precursor
NM_001142800.2	NP_001136272.1	protein eyes shut homolog isoform 1 precursor
NM_198283.1	NP_938024.1	protein eyes shut homolog isoform 3 precursor
NM_001142801.2	NP_001136273.1	protein eyes shut homolog isoform 2 precursor
NM_001292009.1	NP_001278938.1	protein eyes shut homolog isoform 4 precursor

Pathways from BioSystems

Homologies

	EYS	XP_001107053.2
Homo sapiens (human)	EYS	NP_001136272.1
Gallus gallus (chicken)	LOC100858320	XP_003641049.2
Danio rerio (zebrafish)	LOC101886603	XP_005157092.1
Canis lupus familiaris (dog)	EYS	XP_853416.2

Gene Ontology
Bibliography

Related articles in PubMed

EYS mutation update: In silico assessment of 271 reported and 26 novel variants in patients with retinitis pigmentosa.
Messchaert M, Haer-Wigman L, Khan MI, Cremers FPM, Collin RWJ
Human mutation39(2)177-186(2018 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bietti crystalline dystrophy patients with CYP4V2 mutations showed more severe macular choroid atrophy as compared to EYS-related RP patients. These different damage patterns suggest differences in choroidal expression between CYP4V2 and EYS.
Title: CHOROIDAL AND RETINAL ATROPHY OF BIETTI CRYSTALLINE DYSTROPHY PATIENTS WITH CYP4V2 MUTATIONS COMPARED TO RETINITIS PIGMENTOSA PATIENTS WITH EYS MUTATIONS.

The results obtained in this study lead us to speculate that, in photoreceptor cells, EYS could be a protein involved in maintaining the stability of the ciliary axoneme in both rods and cones. The variability of its isoform structure suggests that other roles are also possible and yet to be established
Title: EYS Is a Protein Associated with the Ciliary Axoneme in Rods and Cones.

Taken together, with the targeted NGS approach, we reveal novel EYS mutations and prove the efficiency of targeted NGS in the genetic diagnoses of retinitis pigmentosa.
Title: Targeted next-generation sequencing extends the phenotypic and mutational spectrums for EYS mutations.

our study expands the genotypic spectrums for EYS mutations, and may provide novel insights into the relevant pathogenesis for RP. We also demonstrate targeted next-generation sequencing approach as a valuable tool for genetic diagnosis.
Title: Targeted next-generation sequencing reveals novel EYS mutations in Chinese families with autosomal recessive retinitis pigmentosa.

Advanced retinal degenerative changes with near-total absence of rods and preservation of some perifoveal cones are observed in arRP donor retinas with EYS mutations.
Title: Histopathological comparison of eyes from patients with autosomal recessive retinitis pigmentosa caused by novel EYS mutations.

The following EYS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EYS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27386	NM_001142800.2 Latest version!	Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu25909	NM_198283.1 Latest version!	Homo sapiens eyes shut homolog (EYS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$349.30 $499.00
OHu26927	NM_001142801.2 Latest version!	Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu24604	NM_001292009.1 Latest version!	Homo sapiens eyes shut homolog (EYS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu27386	NM_001142800.1	Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu26927	NM_001142801.1	Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EYS ( NM_001142800.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136272.1 Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27386
Clone ID Related Accession (Same CDS sequence)	NM_001142800.1 , NM_001142800.2
Accession Version	NM_001142800.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FJ416331.1 and BX537914.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FJ416331.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END##

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCTTTATCCA TAAGTGATCC ATCTTTCAGA 
AGCAATGAGT TATCCTGGAT GTCTTTTGCT TCCTTTCATG TTCGAAAAAA GACACATATT 
CAATTGCAGT TTCAGCCTCT CGCTGCAGAT GGTATCCTAT TTTATGCTGC ACAACACTTA 
AAAGCCCAAT CAGGTGATTT TTTATGCATC TCTTTAGTAA ATAGTTCCGT TCAACTTCGC 
TACAACCTTG GCGACAGAAC TATCATTCTA GAAACTCTCC AAAAAGTAAC TATAAACGGA 
AGTACTTGGC ATATAATAAA AGCAGGAAGA GTTGGTGCAG AAGGCTACCT GGATCTAGAT 
GGGATAAATG TAACAGAAAA GGCCTCCACT AAAATGAGTT CTCTGGACAC AAATACAGAC 
TTCTATATTG GAGGAGTATC TTCTTTAAAT CTTGTAAATC CCATGGCAAT AGAAAATGAA 
CCTGTAGGTT TTCAAGGCTG TATCCGACAA GTTATCATAA ATAATCAAGA ATTACAATTA 
ACTGAATTTG GAGCAAAAGG TGGCTCAAAT GTAGGTGACT GTGATGGGAC AGCCTGTGGG 
TACAACACAT GCAGAAATGG AGGTGAATGT ACAGTAAATG GCACAACTTT TTCTTGCAGA 
TGTTTGCCAG ATTGGGCTGG AAATACATGT AACCAGTCTG TGTCCTGTTT GAATAATCTT 
TGCCTCCACC AATCTTTATG TATACCTGAC CAATCATTTT CTTACAGTTG CCTGTGTACT 
TTGGGTTGGG TGGGAAGGTA TTGTGAAAAC AAAACTTCAT TTTCAACTGC AAAATTTATG 
GGTAATTCTT ACATTAAATA CATTGATCCA AATTATAGAA TGAGAAACCT CCAGTTCACT 
ACTATATCCT TAAATTTCAG TACCACTAAA ACAGAAGGTC TAATTGTATG GATGGGAATA 
GCTCAAAATG AAGAAAATGA TTTTCTGGCA ATTGGTCTCC ATAATCAGAC CTTGAAAATA 
GCAGTTAACT TGGGAGAAAG AATCTCTGTG CCTATGAGCT ATAACAATGG CACATTCTGT 
TGTAATAAAT GGCACCATGT AGTTGTAATT CAAAATCAGA CTCTTATCAA GGCCTACATA 
AATAACAGTC TAATTCTTTC CGAGGATATT GATCCACATA AAAACTTTGT GGCTCTAAAC 
TATGATGGCA TTTGTTATCT AGGGGGCTTT GAATATGGTA GAAAGGTAAA TATCGTTACT 
CAAGAGATTT TTAAAACCAA TTTTGTTGGC AAAATTAAAG ATGTTGTATT TTTTCAGGAA 
CCAAAAAACA TTGAACTAAT TAAATTAGAA GGATACAATG TTTATGATGG AGATGAACAA 
AATGAGGTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136272.1
CDS	539..9973
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRCSCSLSYIGRLCVVNVDYCLGNHSISVHGLCLALSHNCNCSGLQRYERNICEIDTEDCKSASCKNGTTSTHLRGYFFRKCVPGFKGTQCEIDIDECASHPCKNGATCIDQPGNYFCQCVPPFKVVDGFSCLCNPGYVGIRCEQDIDDCILNACEHNSTCKDLHLSYQCVCLSDWEGNFCEQESNECKMNPCKNNSTCTDLYKSYRCECTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCRNNSTCLALVDANQHCICREEFEGKNCEIDVKDCLFLSCQDYGDCEDMVNNFRCICRPGFSGSLCEIEINECSSEPCKNNGTCVDLTNRFFCNCEPEYHGPFCELDVNKCKISPCLDEENCVYRTDGYNCLCAPGYTGINCEINLDECLSEPCLHDGVCIDGINHYTCDCKSGFFGTHCETNANDCLSNPCLHGRCTELINEYPCSCDADGTSTQCKIKINDCTSIPCMNEGFCQKSAHGFTCICPRGYTGAYCEKSIDNCAEPELNSVICLNGGICVDGPGHTFDCRCLPGFSGQFCEININECSSSPCLHGADCEDHINGYVCKCQPGWSGHHCENELECIPNSCVHELCMENEPGSTCLCTPGFMTCSIGLLCGDEIRRITCLTPIFQRTDPISTQTYTIPPSETLVSSFPSIKATRIPAIMDTYPVDQGPKQTGIVKHDILPTTGLATLRISTPLESYLLQELIVTRELSAKHSLLSSADVSSSRFLNFGIRDPAQIVQDKTSVSHMPIRTSAATLGFFFPDRRARTPFIMSSLMSDFIFPTQSLLFENCQTVALSATPTTSVIRSIPGADIELNRQSLLSRGFLLIAASISATPVVSRGAQEDIEEYSADSLISRREHWRLLSPSMSPIFPAKVIISKQVTILNSSALHRFSTKAFNPSEYQAITEASSNQRLTNIKSQAADSLRELSQTCATCSMTEIKSSREFSDQVLHSKQSHFYETFWMNSAILASWYALMGAQTITSGHSFSSATEITPSVAFTEVPSLFPSKKSAKRTILSSSLEESITLSSNLDVNLCLDKTCLSIVPSQTISSDLMNSDLTSKMTTDELSVSENILKLLKIRQYGITMGPTEVLNQESLLDMEKSKGSHTLFKLHPSDSSLDFELNLQIYPDVTLKTYSEITHANDFKNNLPPLTGSVPDFSEVTTNVAFYTVSATPALSIQTSSSMSVIRPDWPYFTDYMTSLKKEVKTSSEWSKWELQPSVQYQEFPTASRHLPFTRSLTLSSLESILAPQRLMISDFSCVRYYGDSYLEFQNVALNPQNNISLEFQTFSSYGLLLYVKQDSNLVDGFFIQLFIENGTLKYHFYCPGEAKFKSINTTVRVDNGQKYTLLIRQELDPCNAELTILGRNTQICESINHVLGKPLPKSGSVFIGGFPDLHGKIQMPVPVKNFTGCIEVIEINNWRSFIPSKAVKNYHINNCRSQGFMLSPTASFVDASDVTQGVDTMWTSVSPSVAAPSVCQQDVCHNGGTCHAIFLSSGIVSFQCDCPLHFTGRFCEKDAGLFFPSFNGNSYLELPFLKFVLEKEHNRTVTIYLTIKTNSLNGTILYSNGNNCGKQFLHLFLVEGRPSVKYGCGNSQNILTVSANYSINTNAFTPITIRYTTPVGSPGVVCMIEMTADGKPPVQKKDTEISHASQAYFESMFLGHIPANVQIHKKAGPVYGFRGCILDLQVNNKEFFIIDEARHGKNIENCHVPWCAHHLCRNNGTCISDNENLFCECPRLYSGKLCQFASCENNPCGNGATCVPKSGTDIVCLCPYGRSGPLCTDAINITQPRFSGTDAFGYTSFLAYSRISDISFHYEFHLKFQLANNHSALQNNLIFFTGQKGHGLNGDDFLAVGLLNGSVVYSYNLGSGIASIRSEPLNLSLGVHTVHLGKFFQEGWLKVDDHKNKSIIAPGRLVGLNVFSQFYVGGYSEYTPDLLPNGADFKNGFQGCIFTLQVRTEKDGHFRGLGNPEGHPNAGRSVGQCHASPCSLMKCGNGGTCIESGTSVYCNCTTGWKGSFCTETVSTCDPEHDPPHHCSRGATCISLPHGYTCFCPLGTTGIYCEQALSISDPSFRSNELSWMSFASFHVRKKTHIQLQFQPLAADGILFYAAQHLKAQSGDFLCISLVNSSVQLRYNLGDRTIILETLQKVTINGSTWHIIKAGRVGAEGYLDLDGINVTEKASTKMSSLDTNTDFYIGGVSSLNLVNPMAIENEPVGFQGCIRQVIINNQELQLTEFGAKGGSNVGDCDGTACGYNTCRNGGECTVNGTTFSCRCLPDWAGNTCNQSVSCLNNLCLHQSLCIPDQSFSYSCLCTLGWVGRYCENKTSFSTAKFMGNSYIKYIDPNYRMRNLQFTTISLNFSTTKTEGLIVWMGIAQNEENDFLAIGLHNQTLKIAVNLGERISVPMSYNNGTFCCNKWHHVVVIQNQTLIKAYINNSLILSEDIDPHKNFVALNYDGICYLGGFEYGRKVNIVTQEIFKTNFVGKIKDVVFFQEPKNIELIKLEGYNVYDGDEQNEVT

Target ORF information:

RefSeq Version	NM_001142800.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142800.1

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCTTTATCCA TAAGTGATCC ATCTTTCAGA 
AGCAATGAGT TATCCTGGAT GTCTTTTGCT TCCTTTCATG TTCGAAAAAA GACACATATT 
CAATTGCAGT TTCAGCCTCT CGCTGCAGAT GGTATCCTAT TTTATGCTGC ACAACACTTA 
AAAGCCCAAT CAGGTGATTT TTTATGCATC TCTTTAGTAA ATAGTTCCGT TCAACTTCGC 
TACAACCTTG GCGACAGAAC TATCATTCTA GAAACTCTCC AAAAAGTAAC TATAAACGGA 
AGTACTTGGC ATATAATAAA AGCAGGAAGA GTTGGTGCAG AAGGCTACCT GGATCTAGAT 
GGGATAAATG TAACAGAAAA GGCCTCCACT AAAATGAGTT CTCTGGACAC AAATACAGAC 
TTCTATATTG GAGGAGTATC TTCTTTAAAT CTTGTAAATC CCATGGCAAT AGAAAATGAA 
CCTGTAGGTT TTCAAGGCTG TATCCGACAA GTTATCATAA ATAATCAAGA ATTACAATTA 
ACTGAATTTG GAGCAAAAGG TGGCTCAAAT GTAGGTGACT GTGATGGGAC AGCCTGTGGG 
TACAACACAT GCAGAAATGG AGGTGAATGT ACAGTAAATG GCACAACTTT TTCTTGCAGA 
TGTTTGCCAG ATTGGGCTGG AAATACATGT AACCAGTCTG TGTCCTGTTT GAATAATCTT 
TGCCTCCACC AATCTTTATG TATACCTGAC CAATCATTTT CTTACAGTTG CCTGTGTACT 
TTGGGTTGGG TGGGAAGGTA TTGTGAAAAC AAAACTTCAT TTTCAACTGC AAAATTTATG 
GGTAATTCTT ACATTAAATA CATTGATCCA AATTATAGAA TGAGAAACCT CCAGTTCACT 
ACTATATCCT TAAATTTCAG TACCACTAAA ACAGAAGGTC TAATTGTATG GATGGGAATA 
GCTCAAAATG AAGAAAATGA TTTTCTGGCA ATTGGTCTCC ATAATCAGAC CTTGAAAATA 
GCAGTTAACT TGGGAGAAAG AATCTCTGTG CCTATGAGCT ATAACAATGG CACATTCTGT 
TGTAATAAAT GGCACCATGT AGTTGTAATT CAAAATCAGA CTCTTATCAA GGCCTACATA 
AATAACAGTC TAATTCTTTC CGAGGATATT GATCCACATA AAAACTTTGT GGCTCTAAAC 
TATGATGGCA TTTGTTATCT AGGGGGCTTT GAATATGGTA GAAAGGTAAA TATCGTTACT 
CAAGAGATTT TTAAAACCAA TTTTGTTGGC AAAATTAAAG ATGTTGTATT TTTTCAGGAA 
CCAAAAAACA TTGAACTAAT TAAATTAGAA GGATACAATG TTTATGATGG AGATGAACAA 
AATGAGGTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EYS ( NM_001142800.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136272.1 Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.

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CloneID	OHu27386
Clone ID Related Accession (Same CDS sequence)	NM_001142800.1 , NM_001142800.2
Accession Version	NM_001142800.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450319.4, FJ416331.1 and BX537914.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001142800.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA1970526 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000503581.6/ ENSP00000424243.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END##

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCTTTATCCA TAAGTGATCC ATCTTTCAGA 
AGCAATGAGT TATCCTGGAT GTCTTTTGCT TCCTTTCATG TTCGAAAAAA GACACATATT 
CAATTGCAGT TTCAGCCTCT CGCTGCAGAT GGTATCCTAT TTTATGCTGC ACAACACTTA 
AAAGCCCAAT CAGGTGATTT TTTATGCATC TCTTTAGTAA ATAGTTCCGT TCAACTTCGC 
TACAACCTTG GCGACAGAAC TATCATTCTA GAAACTCTCC AAAAAGTAAC TATAAACGGA 
AGTACTTGGC ATATAATAAA AGCAGGAAGA GTTGGTGCAG AAGGCTACCT GGATCTAGAT 
GGGATAAATG TAACAGAAAA GGCCTCCACT AAAATGAGTT CTCTGGACAC AAATACAGAC 
TTCTATATTG GAGGAGTATC TTCTTTAAAT CTTGTAAATC CCATGGCAAT AGAAAATGAA 
CCTGTAGGTT TTCAAGGCTG TATCCGACAA GTTATCATAA ATAATCAAGA ATTACAATTA 
ACTGAATTTG GAGCAAAAGG TGGCTCAAAT GTAGGTGACT GTGATGGGAC AGCCTGTGGG 
TACAACACAT GCAGAAATGG AGGTGAATGT ACAGTAAATG GCACAACTTT TTCTTGCAGA 
TGTTTGCCAG ATTGGGCTGG AAATACATGT AACCAGTCTG TGTCCTGTTT GAATAATCTT 
TGCCTCCACC AATCTTTATG TATACCTGAC CAATCATTTT CTTACAGTTG CCTGTGTACT 
TTGGGTTGGG TGGGAAGGTA TTGTGAAAAC AAAACTTCAT TTTCAACTGC AAAATTTATG 
GGTAATTCTT ACATTAAATA CATTGATCCA AATTATAGAA TGAGAAACCT CCAGTTCACT 
ACTATATCCT TAAATTTCAG TACCACTAAA ACAGAAGGTC TAATTGTATG GATGGGAATA 
GCTCAAAATG AAGAAAATGA TTTTCTGGCA ATTGGTCTCC ATAATCAGAC CTTGAAAATA 
GCAGTTAACT TGGGAGAAAG AATCTCTGTG CCTATGAGCT ATAACAATGG CACATTCTGT 
TGTAATAAAT GGCACCATGT AGTTGTAATT CAAAATCAGA CTCTTATCAA GGCCTACATA 
AATAACAGTC TAATTCTTTC CGAGGATATT GATCCACATA AAAACTTTGT GGCTCTAAAC 
TATGATGGCA TTTGTTATCT AGGGGGCTTT GAATATGGTA GAAAGGTAAA TATCGTTACT 
CAAGAGATTT TTAAAACCAA TTTTGTTGGC AAAATTAAAG ATGTTGTATT TTTTCAGGAA 
CCAAAAAACA TTGAACTAAT TAAATTAGAA GGATACAATG TTTATGATGG AGATGAACAA 
AATGAGGTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136272.1
CDS	540..9974
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRCSCSLSYIGRLCVVNVDYCLGNHSISVHGLCLALSHNCNCSGLQRYERNICEIDTEDCKSASCKNGTTSTHLRGYFFRKCVPGFKGTQCEIDIDECASHPCKNGATCIDQPGNYFCQCVPPFKVVDGFSCLCNPGYVGIRCEQDIDDCILNACEHNSTCKDLHLSYQCVCLSDWEGNFCEQESNECKMNPCKNNSTCTDLYKSYRCECTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCRNNSTCLALVDANQHCICREEFEGKNCEIDVKDCLFLSCQDYGDCEDMVNNFRCICRPGFSGSLCEIEINECSSEPCKNNGTCVDLTNRFFCNCEPEYHGPFCELDVNKCKISPCLDEENCVYRTDGYNCLCAPGYTGINCEINLDECLSEPCLHDGVCIDGINHYTCDCKSGFFGTHCETNANDCLSNPCLHGRCTELINEYPCSCDADGTSTQCKIKINDCTSIPCMNEGFCQKSAHGFTCICPRGYTGAYCEKSIDNCAEPELNSVICLNGGICVDGPGHTFDCRCLPGFSGQFCEININECSSSPCLHGADCEDHINGYVCKCQPGWSGHHCENELECIPNSCVHELCMENEPGSTCLCTPGFMTCSIGLLCGDEIRRITCLTPIFQRTDPISTQTYTIPPSETLVSSFPSIKATRIPAIMDTYPVDQGPKQTGIVKHDILPTTGLATLRISTPLESYLLQELIVTRELSAKHSLLSSADVSSSRFLNFGIRDPAQIVQDKTSVSHMPIRTSAATLGFFFPDRRARTPFIMSSLMSDFIFPTQSLLFENCQTVALSATPTTSVIRSIPGADIELNRQSLLSRGFLLIAASISATPVVSRGAQEDIEEYSADSLISRREHWRLLSPSMSPIFPAKVIISKQVTILNSSALHRFSTKAFNPSEYQAITEASSNQRLTNIKSQAADSLRELSQTCATCSMTEIKSSREFSDQVLHSKQSHFYETFWMNSAILASWYALMGAQTITSGHSFSSATEITPSVAFTEVPSLFPSKKSAKRTILSSSLEESITLSSNLDVNLCLDKTCLSIVPSQTISSDLMNSDLTSKMTTDELSVSENILKLLKIRQYGITMGPTEVLNQESLLDMEKSKGSHTLFKLHPSDSSLDFELNLQIYPDVTLKTYSEITHANDFKNNLPPLTGSVPDFSEVTTNVAFYTVSATPALSIQTSSSMSVIRPDWPYFTDYMTSLKKEVKTSSEWSKWELQPSVQYQEFPTASRHLPFTRSLTLSSLESILAPQRLMISDFSCVRYYGDSYLEFQNVALNPQNNISLEFQTFSSYGLLLYVKQDSNLVDGFFIQLFIENGTLKYHFYCPGEAKFKSINTTVRVDNGQKYTLLIRQELDPCNAELTILGRNTQICESINHVLGKPLPKSGSVFIGGFPDLHGKIQMPVPVKNFTGCIEVIEINNWRSFIPSKAVKNYHINNCRSQGFMLSPTASFVDASDVTQGVDTMWTSVSPSVAAPSVCQQDVCHNGGTCHAIFLSSGIVSFQCDCPLHFTGRFCEKDAGLFFPSFNGNSYLELPFLKFVLEKEHNRTVTIYLTIKTNSLNGTILYSNGNNCGKQFLHLFLVEGRPSVKYGCGNSQNILTVSANYSINTNAFTPITIRYTTPVGSPGVVCMIEMTADGKPPVQKKDTEISHASQAYFESMFLGHIPANVQIHKKAGPVYGFRGCILDLQVNNKEFFIIDEARHGKNIENCHVPWCAHHLCRNNGTCISDNENLFCECPRLYSGKLCQFASCENNPCGNGATCVPKSGTDIVCLCPYGRSGPLCTDAINITQPRFSGTDAFGYTSFLAYSRISDISFHYEFHLKFQLANNHSALQNNLIFFTGQKGHGLNGDDFLAVGLLNGSVVYSYNLGSGIASIRSEPLNLSLGVHTVHLGKFFQEGWLKVDDHKNKSIIAPGRLVGLNVFSQFYVGGYSEYTPDLLPNGADFKNGFQGCIFTLQVRTEKDGHFRGLGNPEGHPNAGRSVGQCHASPCSLMKCGNGGTCIESGTSVYCNCTTGWKGSFCTETVSTCDPEHDPPHHCSRGATCISLPHGYTCFCPLGTTGIYCEQALSISDPSFRSNELSWMSFASFHVRKKTHIQLQFQPLAADGILFYAAQHLKAQSGDFLCISLVNSSVQLRYNLGDRTIILETLQKVTINGSTWHIIKAGRVGAEGYLDLDGINVTEKASTKMSSLDTNTDFYIGGVSSLNLVNPMAIENEPVGFQGCIRQVIINNQELQLTEFGAKGGSNVGDCDGTACGYNTCRNGGECTVNGTTFSCRCLPDWAGNTCNQSVSCLNNLCLHQSLCIPDQSFSYSCLCTLGWVGRYCENKTSFSTAKFMGNSYIKYIDPNYRMRNLQFTTISLNFSTTKTEGLIVWMGIAQNEENDFLAIGLHNQTLKIAVNLGERISVPMSYNNGTFCCNKWHHVVVIQNQTLIKAYINNSLILSEDIDPHKNFVALNYDGICYLGGFEYGRKVNIVTQEIFKTNFVGKIKDVVFFQEPKNIELIKLEGYNVYDGDEQNEVT

Target ORF information:

RefSeq Version	NM_001142800.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142800.2

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCTTTATCCA TAAGTGATCC ATCTTTCAGA 
AGCAATGAGT TATCCTGGAT GTCTTTTGCT TCCTTTCATG TTCGAAAAAA GACACATATT 
CAATTGCAGT TTCAGCCTCT CGCTGCAGAT GGTATCCTAT TTTATGCTGC ACAACACTTA 
AAAGCCCAAT CAGGTGATTT TTTATGCATC TCTTTAGTAA ATAGTTCCGT TCAACTTCGC 
TACAACCTTG GCGACAGAAC TATCATTCTA GAAACTCTCC AAAAAGTAAC TATAAACGGA 
AGTACTTGGC ATATAATAAA AGCAGGAAGA GTTGGTGCAG AAGGCTACCT GGATCTAGAT 
GGGATAAATG TAACAGAAAA GGCCTCCACT AAAATGAGTT CTCTGGACAC AAATACAGAC 
TTCTATATTG GAGGAGTATC TTCTTTAAAT CTTGTAAATC CCATGGCAAT AGAAAATGAA 
CCTGTAGGTT TTCAAGGCTG TATCCGACAA GTTATCATAA ATAATCAAGA ATTACAATTA 
ACTGAATTTG GAGCAAAAGG TGGCTCAAAT GTAGGTGACT GTGATGGGAC AGCCTGTGGG 
TACAACACAT GCAGAAATGG AGGTGAATGT ACAGTAAATG GCACAACTTT TTCTTGCAGA 
TGTTTGCCAG ATTGGGCTGG AAATACATGT AACCAGTCTG TGTCCTGTTT GAATAATCTT 
TGCCTCCACC AATCTTTATG TATACCTGAC CAATCATTTT CTTACAGTTG CCTGTGTACT 
TTGGGTTGGG TGGGAAGGTA TTGTGAAAAC AAAACTTCAT TTTCAACTGC AAAATTTATG 
GGTAATTCTT ACATTAAATA CATTGATCCA AATTATAGAA TGAGAAACCT CCAGTTCACT 
ACTATATCCT TAAATTTCAG TACCACTAAA ACAGAAGGTC TAATTGTATG GATGGGAATA 
GCTCAAAATG AAGAAAATGA TTTTCTGGCA ATTGGTCTCC ATAATCAGAC CTTGAAAATA 
GCAGTTAACT TGGGAGAAAG AATCTCTGTG CCTATGAGCT ATAACAATGG CACATTCTGT 
TGTAATAAAT GGCACCATGT AGTTGTAATT CAAAATCAGA CTCTTATCAA GGCCTACATA 
AATAACAGTC TAATTCTTTC CGAGGATATT GATCCACATA AAAACTTTGT GGCTCTAAAC 
TATGATGGCA TTTGTTATCT AGGGGGCTTT GAATATGGTA GAAAGGTAAA TATCGTTACT 
CAAGAGATTT TTAAAACCAA TTTTGTTGGC AAAATTAAAG ATGTTGTATT TTTTCAGGAA 
CCAAAAAACA TTGAACTAAT TAAATTAGAA GGATACAATG TTTATGATGG AGATGAACAA 
AATGAGGTTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EYS ( NM_198283.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_938024.1 Homo sapiens eyes shut homolog (EYS), transcript variant 3, mRNA.

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CloneID	OHu25909
Clone ID Related Accession (Same CDS sequence)	NM_198283.1
Accession Version	NM_198283.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY358133.1. On Dec 17, 2003 this sequence version replaced XM_294050.2. Transcript Variant: This variant (3) lacks several exons but contains alternate 5' and 3' exon structure, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (3) has a substantially shorter and unique C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY358133.1, AB593114.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGGTAT TTATGTATCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_938024.1
CDS	398..2182
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRYLCII

Target ORF information:

RefSeq Version	NM_198283.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198283.1

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGGTAT TTATGTATCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EYS ( NM_001142801.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136273.1 Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26927
Clone ID Related Accession (Same CDS sequence)	NM_001142801.1 , NM_001142801.2
Accession Version	NM_001142801.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FJ416331.1, BX537914.1 and AL365217.10. On or before Mar 1, 2009 this sequence version replaced XM_001715536.1, XM_001724639.1, XM_001126128.2, XM_001717769.1, XM_001717771.1, XM_001724715.1. Transcript Variant: This variant (2) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and unique C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGAAGA ATCCTCAACA CGGTGATACC ACACCAAATT 
CAGCAACATA TAGAAAGGTT TATACAGCAT GACCAGGTGG GATTTATCGT AAGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136273.1
CDS	539..2398
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRRILNTVIPHQIQQHIERFIQHDQVGFIVRI

Target ORF information:

RefSeq Version	NM_001142801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142801.1

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGAAGA ATCCTCAACA CGGTGATACC ACACCAAATT 
CAGCAACATA TAGAAAGGTT TATACAGCAT GACCAGGTGG GATTTATCGT AAGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EYS ( NM_001142801.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001136273.1 Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.

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CloneID	OHu26927
Clone ID Related Accession (Same CDS sequence)	NM_001142801.1 , NM_001142801.2
Accession Version	NM_001142801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL450319.4, FJ416331.1, BX537914.1 and AL365217.10. On May 31, 2019 this sequence version replaced NM_001142801.1. Transcript Variant: This variant (2) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in its 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and unique C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537914.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGAAGA ATCCTCAACA CGGTGATACC ACACCAAATT 
CAGCAACATA TAGAAAGGTT TATACAGCAT GACCAGGTGG GATTTATCGT AAGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001136273.1
CDS	540..2399
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRRILNTVIPHQIQQHIERFIQHDQVGFIVRI

Target ORF information:

RefSeq Version	NM_001142801.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001142801.2

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGAAGA ATCCTCAACA CGGTGATACC ACACCAAATT 
CAGCAACATA TAGAAAGGTT TATACAGCAT GACCAGGTGG GATTTATCGT AAGAATATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EYS ( NM_001292009.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278938.1 Homo sapiens eyes shut homolog (EYS), transcript variant 4, mRNA.

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CloneID	OHu24604
Clone ID Related Accession (Same CDS sequence)	NM_001292009.1
Accession Version	NM_001292009.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	protein eyes shut homolog isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FJ416331.1 and FM209056.1. Transcript Variant: This variant (4) includes an additional in-frame exon in the 3' coding region, compared to variant 1, resulting in an isoform (4) that is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FM209056.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2142680, SAMEA2153980 [ECO:0000350] ##Evidence-Data-END##

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCACTTATCC TCATTGTGAT CTTGGAAAAG 
CCAAAACCAG CAGAACGGAA AGTGAAGAAG GAGGCTTTAT CCATAAGTGA TCCATCTTTC 
AGAAGCAATG AGTTATCCTG GATGTCTTTT GCTTCCTTTC ATGTTCGAAA AAAGACACAT 
ATTCAATTGC AGTTTCAGCC TCTCGCTGCA GATGGTATCC TATTTTATGC TGCACAACAC 
TTAAAAGCCC AATCAGGTGA TTTTTTATGC ATCTCTTTAG TAAATAGTTC CGTTCAACTT 
CGCTACAACC TTGGCGACAG AACTATCATT CTAGAAACTC TCCAAAAAGT AACTATAAAC 
GGAAGTACTT GGCATATAAT AAAAGCAGGA AGAGTTGGTG CAGAAGGCTA CCTGGATCTA 
GATGGGATAA ATGTAACAGA AAAGGCCTCC ACTAAAATGA GTTCTCTGGA CACAAATACA 
GACTTCTATA TTGGAGGAGT ATCTTCTTTA AATCTTGTAA ATCCCATGGC AATAGAAAAT 
GAACCTGTAG GTTTTCAAGG CTGTATCCGA CAAGTTATCA TAAATAATCA AGAATTACAA 
TTAACTGAAT TTGGAGCAAA AGGTGGCTCA AATGTAGGTG ACTGTGATGG GACAGCCTGT 
GGGTACAACA CATGCAGAAA TGGAGGTGAA TGTACAGTAA ATGGCACAAC TTTTTCTTGC 
AGATGTTTGC CAGATTGGGC TGGAAATACA TGTAACCAGT CTGTGTCCTG TTTGAATAAT 
CTTTGCCTCC ACCAATCTTT ATGTATACCT GACCAATCAT TTTCTTACAG TTGCCTGTGT 
ACTTTGGGTT GGGTGGGAAG GTATTGTGAA AACAAAACTT CATTTTCAAC TGCAAAATTT 
ATGGGTAATT CTTACATTAA ATACATTGAT CCAAATTATA GAATGAGAAA CCTCCAGTTC 
ACTACTATAT CCTTAAATTT CAGTACCACT AAAACAGAAG GTCTAATTGT ATGGATGGGA 
ATAGCTCAAA ATGAAGAAAA TGATTTTCTG GCAATTGGTC TCCATAATCA GACCTTGAAA 
ATAGCAGTTA ACTTGGGAGA AAGAATCTCT GTGCCTATGA GCTATAACAA TGGCACATTC 
TGTTGTAATA AATGGCACCA TGTAGTTGTA ATTCAAAATC AGACTCTTAT CAAGGCCTAC 
ATAAATAACA GTCTAATTCT TTCCGAGGAT ATTGATCCAC ATAAAAACTT TGTGGCTCTA 
AACTATGATG GCATTTGTTA TCTAGGGGGC TTTGAATATG GTAGAAAGGT AAATATCGTT 
ACTCAAGAGA TTTTTAAAAC CAATTTTGTT GGCAAAATTA AAGATGTTGT ATTTTTTCAG 
GAACCAAAAA ACATTGAACT AATTAAATTA GAAGGATACA ATGTTTATGA TGGAGATGAA 
CAAAATGAGG TTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278938.1
CDS	539..10036
Translation	MTDKSIVILSLMVFHSSFINGKTCRRQLVEEWHPQPSSYVVNWTLTENICLDFYRDCWFLGVNTKIDTSGNQAVPQICPLQIQLGDILVISSEPSLQFPEINLMNVSETSFVGCVQNTTTEDQLLFGCRLKGMHTVNSKWLSVGTHYFITVMASGPSPCPLGLRLNVTVKQQFCQESLSSEFCSGHGKCLSEAWSKTYSCHCQPPFSGKYCQELDACSFKPCKNNGSCINKRENWDEQAYECVCHPPFTGKNCSEIIGQCQPHVCFHGNCSNITSNSFICECDEQFSGPFCEVSAKPCVSLLFWKRGICPNSSSAYTYECPKGSSSQNGETDVSEFSLVPCQNGTDCIKISNDVMCICSPIFTDLLCKSIQTSCESFPLRNNATCKKCEKDYPCSCISGFTEKNCEKAIDHCKLLSINCLNEEWCFNIIGRFKYVCIPGCTKNPCWFLKNVYLIHQHLCYCGVTFHGICQDKGPAQFEYVWQLGFAGSEGEKCQGVIDAYFFLAANCTEDATYVNDPEDNNSSCWFPHEGTKEICANGCSCLSEEDSQEYRYLCFLRWAGNMYLENTTDDQENECQHEAVCKDEINRPRCSCSLSYIGRLCVVNVDYCLGNHSISVHGLCLALSHNCNCSGLQRYERNICEIDTEDCKSASCKNGTTSTHLRGYFFRKCVPGFKGTQCEIDIDECASHPCKNGATCIDQPGNYFCQCVPPFKVVDGFSCLCNPGYVGIRCEQDIDDCILNACEHNSTCKDLHLSYQCVCLSDWEGNFCEQESNECKMNPCKNNSTCTDLYKSYRCECTSGWTGQNCSEEINECDSDPCMNGGLCHESTIPGQFVCLCPPLYTGQFCHQRYNLCDLLHNPCRNNSTCLALVDANQHCICREEFEGKNCEIDVKDCLFLSCQDYGDCEDMVNNFRCICRPGFSGSLCEIEINECSSEPCKNNGTCVDLTNRFFCNCEPEYHGPFCELDVNKCKISPCLDEENCVYRTDGYNCLCAPGYTGINCEINLDECLSEPCLHDGVCIDGINHYTCDCKSGFFGTHCETNANDCLSNPCLHGRCTELINEYPCSCDADGTSTQCKIKINDCTSIPCMNEGFCQKSAHGFTCICPRGYTGAYCEKSIDNCAEPELNSVICLNGGICVDGPGHTFDCRCLPGFSGQFCEININECSSSPCLHGADCEDHINGYVCKCQPGWSGHHCENELECIPNSCVHELCMENEPGSTCLCTPGFMTCSIGLLCGDEIRRITCLTPIFQRTDPISTQTYTIPPSETLVSSFPSIKATRIPAIMDTYPVDQGPKQTGIVKHDILPTTGLATLRISTPLESYLLQELIVTRELSAKHSLLSSADVSSSRFLNFGIRDPAQIVQDKTSVSHMPIRTSAATLGFFFPDRRARTPFIMSSLMSDFIFPTQSLLFENCQTVALSATPTTSVIRSIPGADIELNRQSLLSRGFLLIAASISATPVVSRGAQEDIEEYSADSLISRREHWRLLSPSMSPIFPAKVIISKQVTILNSSALHRFSTKAFNPSEYQAITEASSNQRLTNIKSQAADSLRELSQTCATCSMTEIKSSREFSDQVLHSKQSHFYETFWMNSAILASWYALMGAQTITSGHSFSSATEITPSVAFTEVPSLFPSKKSAKRTILSSSLEESITLSSNLDVNLCLDKTCLSIVPSQTISSDLMNSDLTSKMTTDELSVSENILKLLKIRQYGITMGPTEVLNQESLLDMEKSKGSHTLFKLHPSDSSLDFELNLQIYPDVTLKTYSEITHANDFKNNLPPLTGSVPDFSEVTTNVAFYTVSATPALSIQTSSSMSVIRPDWPYFTDYMTSLKKEVKTSSEWSKWELQPSVQYQEFPTASRHLPFTRSLTLSSLESILAPQRLMISDFSCVRYYGDSYLEFQNVALNPQNNISLEFQTFSSYGLLLYVKQDSNLVDGFFIQLFIENGTLKYHFYCPGEAKFKSINTTVRVDNGQKYTLLIRQELDPCNAELTILGRNTQICESINHVLGKPLPKSGSVFIGGFPDLHGKIQMPVPVKNFTGCIEVIEINNWRSFIPSKAVKNYHINNCRSQGFMLSPTASFVDASDVTQGVDTMWTSVSPSVAAPSVCQQDVCHNGGTCHAIFLSSGIVSFQCDCPLHFTGRFCEKDAGLFFPSFNGNSYLELPFLKFVLEKEHNRTVTIYLTIKTNSLNGTILYSNGNNCGKQFLHLFLVEGRPSVKYGCGNSQNILTVSANYSINTNAFTPITIRYTTPVGSPGVVCMIEMTADGKPPVQKKDTEISHASQAYFESMFLGHIPANVQIHKKAGPVYGFRGCILDLQVNNKEFFIIDEARHGKNIENCHVPWCAHHLCRNNGTCISDNENLFCECPRLYSGKLCQFASCENNPCGNGATCVPKSGTDIVCLCPYGRSGPLCTDAINITQPRFSGTDAFGYTSFLAYSRISDISFHYEFHLKFQLANNHSALQNNLIFFTGQKGHGLNGDDFLAVGLLNGSVVYSYNLGSGIASIRSEPLNLSLGVHTVHLGKFFQEGWLKVDDHKNKSIIAPGRLVGLNVFSQFYVGGYSEYTPDLLPNGADFKNGFQGCIFTLQVRTEKDGHFRGLGNPEGHPNAGRSVGQCHASPCSLMKCGNGGTCIESGTSVYCNCTTGWKGSFCTETVSTCDPEHDPPHHCSRGATCISLPHGYTCFCPLGTTGIYCEQALILIVILEKPKPAERKVKKEALSISDPSFRSNELSWMSFASFHVRKKTHIQLQFQPLAADGILFYAAQHLKAQSGDFLCISLVNSSVQLRYNLGDRTIILETLQKVTINGSTWHIIKAGRVGAEGYLDLDGINVTEKASTKMSSLDTNTDFYIGGVSSLNLVNPMAIENEPVGFQGCIRQVIINNQELQLTEFGAKGGSNVGDCDGTACGYNTCRNGGECTVNGTTFSCRCLPDWAGNTCNQSVSCLNNLCLHQSLCIPDQSFSYSCLCTLGWVGRYCENKTSFSTAKFMGNSYIKYIDPNYRMRNLQFTTISLNFSTTKTEGLIVWMGIAQNEENDFLAIGLHNQTLKIAVNLGERISVPMSYNNGTFCCNKWHHVVVIQNQTLIKAYINNSLILSEDIDPHKNFVALNYDGICYLGGFEYGRKVNIVTQEIFKTNFVGKIKDVVFFQEPKNIELIKLEGYNVYDGDEQNEVT

Target ORF information:

RefSeq Version	NM_001292009.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eyes shut homolog (EYS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292009.1

ORF Insert Sequence:

ATGACTGACA AATCAATCGT CATTCTGAGC CTGATGGTTT TTCACAGCTC TTTCATAAAT 
GGAAAAACAT GTAGACGGCA ATTGGTGGAA GAATGGCATC CACAACCCTC ATCATATGTG 
GTAAATTGGA CACTAACAGA AAACATCTGC TTGGACTTCT ACAGAGATTG CTGGTTTTTG 
GGTGTAAACA CTAAAATAGA TACTTCAGGC AATCAAGCTG TTCCCCAGAT TTGCCCTTTG 
CAAATTCAAT TAGGAGATAT CCTTGTAATT TCTTCTGAAC CATCTCTTCA ATTTCCAGAA 
ATAAATTTGA TGAATGTTTC TGAAACATCT TTCGTTGGCT GTGTGCAAAA TACCACAACG 
GAAGATCAGT TACTTTTTGG CTGCAGACTA AAAGGAATGC ACACTGTTAA TTCTAAGTGG 
CTGAGTGTTG GGACACATTA TTTTATCACA GTTATGGCAA GTGGTCCATC ACCTTGTCCA 
CTGGGACTTC GACTAAATGT GACAGTGAAA CAGCAGTTCT GCCAGGAATC TCTGAGTTCA 
GAATTTTGCT CTGGTCATGG TAAATGTCTT AGTGAAGCTT GGAGCAAGAC ATATAGCTGC 
CATTGCCAGC CTCCATTTTC TGGAAAATAC TGCCAGGAAC TTGATGCATG TTCTTTTAAA 
CCATGTAAAA ATAATGGCAG TTGCATTAAT AAAAGAGAAA ATTGGGATGA GCAAGCATAT 
GAATGTGTCT GTCACCCACC ATTTACAGGA AAGAATTGCT CAGAAATAAT TGGCCAGTGT 
CAACCACATG TCTGTTTCCA TGGAAACTGC AGCAATATTA CTTCAAATAG TTTCATTTGT 
GAATGTGATG AGCAATTTTC AGGTCCATTC TGTGAGGTGT CAGCAAAACC TTGTGTTTCT 
CTGCTTTTTT GGAAAAGAGG AATTTGCCCA AATAGCAGTT CTGCTTATAC TTATGAATGC 
CCAAAAGGAT CTTCCAGCCA AAATGGTGAA ACTGATGTCA GTGAGTTTTC ATTAGTACCA 
TGTCAGAATG GTACTGACTG CATCAAAATA TCAAATGATG TTATGTGCAT CTGTTCACCA 
ATATTTACAG ATTTGCTTTG TAAGAGCATT CAAACATCAT GTGAGTCATT TCCTTTGAGG 
AATAATGCTA CATGTAAGAA ATGTGAGAAA GATTATCCTT GCAGCTGTAT ATCAGGATTT 
ACTGAAAAAA ACTGTGAGAA AGCAATTGAC CACTGTAAAC TGCTCAGCAT CAACTGTCTG 
AATGAAGAAT GGTGTTTCAA TATAATTGGA AGATTCAAAT ATGTATGCAT TCCAGGGTGC 
ACAAAAAATC CATGTTGGTT TTTGAAGAAT GTTTACCTAA TTCATCAACA CCTCTGCTAC 
TGTGGAGTCA CCTTCCATGG TATTTGCCAA GATAAAGGTC CTGCTCAATT TGAATATGTG 
TGGCAATTGG GATTTGCAGG ATCTGAAGGC GAAAAGTGCC AAGGGGTTAT TGATGCCTAT 
TTCTTTCTGG CTGCAAACTG CACTGAAGAT GCAACCTATG TGAACGATCC TGAAGATAAT 
AATTCTTCAT GTTGGTTCCC ACATGAAGGC ACAAAAGAGA TTTGTGCAAA TGGATGCAGT 
TGTTTGAGTG AAGAAGACAG TCAGGAATAT CGGTATCTAT GTTTTCTCAG ATGGGCTGGC 
AACATGTATC TGGAAAATAC AACTGATGAT CAAGAAAATG AGTGTCAACA TGAAGCTGTT 
TGTAAAGATG AAATTAATAG ACCCAGATGC AGCTGTTCTC TTAGTTACAT TGGCAGATTG 
TGTGTTGTCA ATGTTGACTA TTGCTTAGGG AACCACAGTA TATCAGTGCA TGGCCTCTGC 
CTGGCCCTTT CGCACAATTG TAACTGTAGC GGTCTGCAAA GATATGAAAG GAACATCTGT 
GAGATAGATA CTGAAGACTG CAAATCTGCG TCCTGCAAAA ATGGAACAAC TAGTACACAT 
TTAAGGGGAT ATTTCTTCCG CAAGTGTGTC CCAGGATTTA AAGGTACGCA ATGTGAAATT 
GATATAGATG AGTGTGCTTC ACATCCCTGC AAAAATGGAG CCACCTGCAT TGACCAACCT 
GGTAATTACT TCTGCCAGTG TGTGCCTCCA TTTAAAGTGG TTGATGGCTT TTCCTGCTTA 
TGCAATCCAG GCTATGTTGG GATAAGATGT GAACAGGACA TTGATGACTG CATCCTGAAT 
GCCTGTGAGC ACAATTCTAC CTGCAAAGAC CTGCATCTCA GCTACCAGTG TGTGTGCCTA 
TCTGATTGGG AAGGAAATTT TTGTGAACAA GAATCCAATG AGTGTAAAAT GAATCCTTGC 
AAGAACAATT CCACCTGTAC TGACCTTTAC AAAAGCTATC GATGTGAGTG TACATCTGGA 
TGGACTGGAC AGAACTGTAG TGAAGAAATA AATGAATGCG ACTCTGATCC ATGCATGAAT 
GGAGGTCTTT GTCATGAATC TACCATCCCT GGACAATTTG TATGTCTGTG CCCACCCCTT 
TATACTGGAC AATTTTGCCA CCAACGCTAT AACCTTTGTG ACCTACTTCA TAACCCTTGC 
AGAAACAACT CAACATGCTT AGCTTTGGTA GACGCAAATC AGCATTGTAT TTGTAGAGAA 
GAATTTGAAG GTAAAAACTG TGAAATTGAT GTGAAAGACT GCCTCTTCCT TTCCTGCCAG 
GATTATGGTG ACTGTGAAGA TATGGTCAAC AATTTCAGGT GTATTTGCAG ACCTGGGTTT 
TCTGGATCTC TGTGTGAAAT TGAAATTAAT GAATGTTCCT CTGAACCTTG CAAAAATAAT 
GGAACATGTG TGGATCTGAC AAACAGATTT TTTTGTAATT GTGAACCTGA GTACCATGGG 
CCCTTCTGTG AACTTGATGT AAATAAATGT AAAATCTCAC CTTGTCTAGA TGAAGAAAAT 
TGTGTCTACA GGACTGATGG ATACAACTGC CTCTGTGCCC CTGGTTATAC AGGCATCAAC 
TGTGAAATAA ATCTAGATGA ATGCCTATCA GAGCCCTGTC TCCATGATGG AGTTTGTATC 
GATGGCATCA ATCATTATAC CTGTGACTGC AAGAGTGGGT TTTTTGGAAC ACACTGTGAA 
ACAAACGCCA ATGATTGCCT TTCAAATCCT TGTCTACATG GAAGGTGCAC AGAACTTATT 
AATGAATATC CATGTTCATG TGATGCAGAT GGGACTAGCA CACAATGTAA GATCAAAATT 
AATGACTGCA CATCAATCCC TTGTATGAAT GAAGGCTTCT GTCAGAAGTC AGCACATGGA 
TTTACTTGCA TTTGCCCACG TGGATACACT GGTGCATACT GTGAAAAAAG CATTGATAAT 
TGTGCTGAGC CTGAACTTAA TTCAGTCATC TGTCTTAATG GAGGGATCTG TGTTGATGGG 
CCTGGACATA CTTTTGACTG CAGATGTCTT CCTGGATTTT CTGGTCAATT TTGTGAAATT 
AATATAAATG AATGCTCTTC ATCACCATGT CTACATGGTG CAGACTGTGA AGATCACATC 
AATGGATATG TTTGCAAATG CCAACCAGGA TGGTCTGGAC ACCACTGTGA GAATGAGCTT 
GAGTGCATTC CCAACTCATG TGTTCATGAA CTCTGCATGG AGAATGAACC TGGCTCGACA 
TGTTTATGCA CACCTGGATT TATGACCTGC TCCATTGGGC TTCTTTGTGG TGATGAAATA 
AGGAGAATTA CCTGTTTAAC TCCCATCTTT CAAAGAACAG ATCCCATTTC CACACAGACA 
TATACAATTC CCCCTTCTGA GACTTTGGTC AGCAGCTTTC CATCTATAAA GGCTACTAGA 
ATACCAGCCA TAATGGACAC TTACCCAGTT GATCAAGGTC CAAAACAGAC AGGCATTGTC 
AAGCACGACA TTCTCCCAAC CACTGGTTTG GCAACATTAA GAATTAGCAC ACCCTTGGAA 
AGCTACTTAC TCCAAGAACT GATTGTCACT AGAGAGCTTT CAGCAAAACA CAGTCTTCTT 
TCTTCCGCAG ATGTTTCCTC TTCTCGATTC CTGAATTTTG GTATTCGTGA CCCAGCACAA 
ATTGTCCAGG ACAAAACATC GGTATCACAT ATGCCTATTC GAACTTCAGC AGCCACACTA 
GGTTTCTTTT TTCCTGATAG GAGAGCAAGG ACCCCATTTA TCATGTCTTC CTTAATGTCA 
GATTTTATTT TTCCTACACA ATCTTTATTA TTTGAGAACT GTCAGACTGT TGCTTTATCT 
GCTACCCCAA CGACTTCAGT AATTAGAAGC ATTCCAGGGG CTGATATTGA GCTAAACAGG 
CAGTCATTAC TCTCCCGTGG ATTCCTGCTT ATAGCTGCCT CCATAAGTGC AACTCCAGTT 
GTCTCTAGGG GGGCTCAAGA GGATATTGAA GAATATTCAG CTGATTCTTT AATTTCAAGA 
AGAGAGCACT GGAGATTGCT CAGCCCCTCG ATGTCTCCCA TTTTTCCTGC TAAGGTAATT 
ATATCTAAAC AGGTAACCAT CTTAAACTCA TCAGCTCTGC ACCGGTTCAG TACAAAAGCC 
TTCAATCCCA GTGAATATCA GGCTATTACT GAGGCTTCAA GCAACCAGAG ACTCACAAAC 
ATCAAATCAC AGGCTGCTGA TTCTTTAAGA GAATTAAGCC AAACATGTGC AACATGTTCT 
ATGACTGAAA TAAAATCCTC TCGTGAATTC TCAGATCAAG TTTTGCATAG CAAACAGTCC 
CACTTTTATG AGACATTCTG GATGAACTCA GCGATATTAG CCAGCTGGTA TGCACTAATG 
GGAGCTCAAA CTATCACTTC TGGGCATTCA TTTTCTTCTG CTACTGAAAT AACACCATCA 
GTGGCATTCA CAGAAGTGCC ATCTTTATTT CCTTCTAAAA AGAGTGCAAA AAGAACAATT 
TTATCCTCAT CCTTGGAAGA ATCCATTACC CTATCAAGTA ATTTGGATGT TAATTTATGT 
TTGGATAAGA CTTGTTTATC CATTGTCCCT TCACAAACTA TCTCTTCAGA CTTGATGAAT 
TCTGATTTGA CTTCAAAAAT GACTACTGAT GAACTGTCAG TATCAGAAAA CATTTTAAAA 
CTATTGAAAA TAAGACAATA TGGCATAACT ATGGGACCCA CTGAGGTACT AAATCAAGAG 
AGCTTATTGG ACATGGAAAA AAGTAAAGGA TCTCATACAC TGTTCAAACT TCACCCAAGT 
GATAGTTCTC TGGATTTTGA GTTAAACTTA CAAATTTATC CGGATGTTAC TTTAAAGACA 
TATTCAGAAA TTACACATGC AAATGACTTC AAAAATAATC TGCCACCATT GACAGGCTCA 
GTGCCTGATT TTTCAGAAGT CACCACCAAT GTTGCATTTT ATACAGTTTC AGCAACTCCA 
GCACTTTCAA TACAGACGTC TTCCTCCATG TCTGTAATTA GGCCAGATTG GCCATATTTT 
ACAGATTATA TGACCTCTCT TAAAAAAGAG GTCAAGACTT CTTCAGAATG GTCCAAATGG 
GAACTTCAGC CTAGTGTGCA ATATCAGGAA TTTCCCACTG CAAGCCGGCA TCTTCCCTTC 
ACTAGATCTC TTACTTTGTC TTCACTGGAA TCCATTCTGG CACCTCAACG GCTGATGATT 
TCTGATTTCA GTTGTGTTCG TTATTATGGA GATTCTTATC TAGAATTTCA GAATGTGGCT 
TTAAATCCAC AAAATAACAT CTCCCTAGAA TTTCAGACCT TCAGCTCCTA TGGACTTCTG 
CTGTATGTCA AGCAAGACTC AAATTTAGTA GATGGATTTT TTATTCAATT GTTTATTGAA 
AATGGTACTT TAAAGTACCA CTTTTACTGT CCTGGTGAAG CAAAATTTAA AAGCATTAAT 
ACTACTGTTA GAGTGGACAA CGGGCAAAAG TATACACTGC TTATCAGGCA AGAATTGGAT 
CCATGTAACG CTGAGCTGAC TATTTTAGGG AGGAATACAC AAATATGCGA ATCTATCAAT 
CATGTACTCG GAAAACCCCT GCCAAAATCA GGATCTGTCT TCATTGGTGG ATTTCCAGAC 
CTTCATGGGA AAATCCAGAT GCCTGTACCA GTCAAGAATT TTACTGGCTG CATAGAAGTT 
ATAGAAATAA ATAACTGGAG ATCTTTCATT CCATCCAAGG CAGTGAAAAA CTATCACATT 
AACAATTGCA GATCCCAGGG ATTTATGCTG TCTCCAACAG CCTCCTTTGT TGATGCTTCT 
GATGTGACAC AAGGGGTTGA TACCATGTGG ACTTCTGTCA GCCCCTCTGT TGCAGCACCC 
TCTGTGTGCC AGCAGGATGT ATGCCACAAT GGAGGCACAT GCCATGCCAT CTTCCTCTCC 
AGTGGCATAG TGTCATTCCA ATGTGACTGT CCACTACATT TCACTGGCCG CTTCTGTGAA 
AAAGATGCAG GTTTATTCTT TCCATCTTTC AATGGGAATT CCTATTTAGA ACTGCCCTTT 
TTGAAGTTTG TCCTGGAGAA GGAACATAAC AGAACTGTTA CCATCTACTT GACTATAAAA 
ACAAACAGTT TAAATGGAAC TATTCTTTAC AGTAATGGGA ATAATTGTGG AAAGCAGTTT 
CTTCATTTAT TTCTTGTGGA AGGAAGGCCA TCAGTTAAAT ATGGGTGTGG AAATTCTCAA 
AATATTTTGA CTGTTTCTGC TAATTACAGC ATTAACACAA ATGCATTCAC CCCTATCACA 
ATACGCTACA CAACGCCTGT TGGCAGCCCT GGAGTTGTTT GTATGATTGA AATGACTGCA 
GATGGAAAAC CTCCAGTACA GAAGAAAGAC ACAGAGATTT CCCATGCCTC TCAGGCATAT 
TTTGAATCAA TGTTCCTTGG CCATATTCCT GCAAATGTTC AAATTCATAA GAAAGCAGGT 
CCTGTTTATG GGTTCAGGGG CTGCATTCTA GACCTTCAAG TAAACAACAA AGAATTCTTC 
ATCATCGATG AAGCACGACA TGGAAAGAAT ATTGAGAACT GCCACGTCCC TTGGTGTGCT 
CATCATCTGT GCCGCAACAA TGGCACCTGC ATCAGTGATA ATGAAAATCT GTTTTGTGAG 
TGTCCAAGGC TGTATTCAGG CAAGCTGTGC CAGTTTGCAA GTTGTGAAAA CAACCCATGT 
GGAAATGGTG CCACCTGTGT TCCAAAATCC GGAACAGATA TTGTCTGCCT CTGCCCATAT 
GGGAGGTCTG GACCCCTCTG CACTGATGCT ATTAATATTA CTCAGCCAAG GTTCAGTGGC 
ACAGATGCCT TTGGATACAC CTCATTCCTG GCTTATTCAC GGATCTCAGA CATCAGCTTC 
CATTATGAAT TCCACCTGAA GTTTCAGCTG GCAAACAACC ACTCAGCACT GCAAAATAAC 
TTGATATTTT TTACTGGACA GAAAGGCCAT GGGTTGAATG GCGATGACTT CCTGGCTGTG 
GGCCTGCTCA ATGGCAGTGT GGTTTATAGT TATAACCTGG GGTCTGGCAT AGCAAGCATC 
AGGAGCGAGC CCCTCAATCT GAGCCTTGGA GTCCACACTG TTCATCTGGG CAAGTTCTTC 
CAAGAGGGCT GGCTGAAGGT AGATGATCAT AAAAATAAAT CCATTATCGC CCCAGGAAGA 
CTGGTTGGTC TCAATGTCTT CAGTCAGTTT TATGTAGGTG GCTACAGTGA ATACACTCCA 
GATCTCTTAC CAAATGGAGC AGATTTTAAA AATGGTTTTC AAGGCTGTAT TTTCACTCTT 
CAAGTTCGCA CTGAGAAGGA TGGCCATTTC AGAGGACTGG GAAATCCTGA GGGCCACCCA 
AATGCTGGAC GCAGTGTTGG CCAGTGTCAT GCTTCTCCCT GCAGTTTAAT GAAATGTGGC 
AATGGTGGGA CATGCATAGA GAGTGGAACT AGTGTTTACT GCAATTGTAC CACTGGGTGG 
AAAGGATCAT TCTGCACAGA GACAGTTTCT ACCTGTGATC CTGAACATGA CCCTCCACAC 
CACTGTAGCA GAGGAGCAAC CTGCATTTCA TTACCTCATG GATACACCTG TTTCTGTCCT 
CTAGGAACCA CTGGAATCTA CTGTGAACAA GCACTTATCC TCATTGTGAT CTTGGAAAAG 
CCAAAACCAG CAGAACGGAA AGTGAAGAAG GAGGCTTTAT CCATAAGTGA TCCATCTTTC 
AGAAGCAATG AGTTATCCTG GATGTCTTTT GCTTCCTTTC ATGTTCGAAA AAAGACACAT 
ATTCAATTGC AGTTTCAGCC TCTCGCTGCA GATGGTATCC TATTTTATGC TGCACAACAC 
TTAAAAGCCC AATCAGGTGA TTTTTTATGC ATCTCTTTAG TAAATAGTTC CGTTCAACTT 
CGCTACAACC TTGGCGACAG AACTATCATT CTAGAAACTC TCCAAAAAGT AACTATAAAC 
GGAAGTACTT GGCATATAAT AAAAGCAGGA AGAGTTGGTG CAGAAGGCTA CCTGGATCTA 
GATGGGATAA ATGTAACAGA AAAGGCCTCC ACTAAAATGA GTTCTCTGGA CACAAATACA 
GACTTCTATA TTGGAGGAGT ATCTTCTTTA AATCTTGTAA ATCCCATGGC AATAGAAAAT 
GAACCTGTAG GTTTTCAAGG CTGTATCCGA CAAGTTATCA TAAATAATCA AGAATTACAA 
TTAACTGAAT TTGGAGCAAA AGGTGGCTCA AATGTAGGTG ACTGTGATGG GACAGCCTGT 
GGGTACAACA CATGCAGAAA TGGAGGTGAA TGTACAGTAA ATGGCACAAC TTTTTCTTGC 
AGATGTTTGC CAGATTGGGC TGGAAATACA TGTAACCAGT CTGTGTCCTG TTTGAATAAT 
CTTTGCCTCC ACCAATCTTT ATGTATACCT GACCAATCAT TTTCTTACAG TTGCCTGTGT 
ACTTTGGGTT GGGTGGGAAG GTATTGTGAA AACAAAACTT CATTTTCAAC TGCAAAATTT 
ATGGGTAATT CTTACATTAA ATACATTGAT CCAAATTATA GAATGAGAAA CCTCCAGTTC 
ACTACTATAT CCTTAAATTT CAGTACCACT AAAACAGAAG GTCTAATTGT ATGGATGGGA 
ATAGCTCAAA ATGAAGAAAA TGATTTTCTG GCAATTGGTC TCCATAATCA GACCTTGAAA 
ATAGCAGTTA ACTTGGGAGA AAGAATCTCT GTGCCTATGA GCTATAACAA TGGCACATTC 
TGTTGTAATA AATGGCACCA TGTAGTTGTA ATTCAAAATC AGACTCTTAT CAAGGCCTAC 
ATAAATAACA GTCTAATTCT TTCCGAGGAT ATTGATCCAC ATAAAAACTT TGTGGCTCTA 
AACTATGATG GCATTTGTTA TCTAGGGGGC TTTGAATATG GTAGAAAGGT AAATATCGTT 
ACTCAAGAGA TTTTTAAAAC CAATTTTGTT GGCAAAATTA AAGATGTTGT ATTTTTTCAG 
GAACCAAAAA ACATTGAACT AATTAAATTA GAAGGATACA ATGTTTATGA TGGAGATGAA 
CAAAATGAGG TTACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EYS mutation update: In silico assessment of 271 reported and 26 novel variants in patients with retinitis pigmentosa.
Human mutation39(2)177-186(2018 Feb)
Messchaert M,Haer-Wigman L,Khan MI,Cremers FPM,Collin RWJ

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EYS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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