RSPH4A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RSPH4A
Entrez Gene ID	345895
Full Name	radial spoke head 4 homolog A
Synonyms	CILD11,RSHL3,RSPH6B,dJ412I7.1
General protein information	Preferred Names radial spoke head 4 homolog A Names radial spoke head protein 4 homolog A radial spoke head-like protein 3 radial spokehead-like 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q22.1
Summary	This gene encodes a protein that appears to be a component the radial spoke head, as determined by homology to similar proteins in the biflagellate alga Chlamydomonas reinhardtii and other ciliates. Radial spokes, which are regularly spaced along cilia, sperm, and flagella axonemes, consist of a thin 'stalk' and a bulbous 'head' that form a signal transduction scaffold between the central pair of microtubules and dynein. Mutations in this gene cause primary ciliary dyskinesia 1, a disease arising from dysmotility of motile cilia and sperm. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009].
Disorder MIM:	612647
Disorder Html:	Ciliary dyskinesia, primary, 11, 612649 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017010826.1	XP_016866315.1	radial spoke head protein 4 homolog A isoform X1
NM_001161664.1	NP_001155136.1	radial spoke head protein 4 homolog A isoform 2
NM_001010892.3	NP_001010892.1	radial spoke head protein 4 homolog A isoform 1
NM_001010892.2	NP_001010892.1	radial spoke head protein 4 homolog A isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RSPH4A	XP_541210.2
Mus musculus (house mouse)	Rsph4a	NP_001156429.1
Gallus gallus (chicken)	RSPH4A	XP_420671.4
Bos taurus (cattle)	RSPH4A	XP_002690133.1
Pan troglodytes (chimpanzee)	RSPH4A	XP_001162376.1
Danio rerio (zebrafish)	LOC100148286	XP_001923770.1
Xenopus tropicalis (tropical clawed frog)	rsph6a	NP_001016760.1
Homo sapiens (human)	RSPH4A	NP_001010892.1
	RSPH4A	XP_001111183.1
Rattus norvegicus (Norway rat)	Rsph4a	XP_003752013.1

Gene Ontology
Bibliography

Related articles in PubMed

A targeted next-generation sequencing panel reveals novel mutations in Japanese patients with primary ciliary dyskinesia.
Takeuchi K, Kitano M, Kiyotoshi H, Ikegami K, Ogawa S, Ikejiri M, Nagao M, Fujisawa T, Nakatani K
Auris, nasus, larynx(2017 Sep)

Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.
Frommer A, Hjeij R, Loges NT, Edelbusch C, Jahnke C, Raidt J, Werner C, Wallmeier J, Gro?e-Onnebrink J, Olbrich H, Cindri? S, Jaspers M, Boon M, Memari Y, Durbin R, Kolb-Kokocinski A, Sauer S, Marthin JK, Nielsen KG, Amirav I, Elias N, Kerem E, Shoseyov D, Haeffner K, Omran H
American journal of respiratory cell and molecular biology53(4)563-73(2015 Oct)

Founder mutation in RSPH4A identified in patients of Hispanic descent with primary ciliary dyskinesia.
Daniels ML, Leigh MW, Davis SD, Armstrong MC, Carson JL, Hazucha M, Dell SD, Eriksson M, Collins FS, Knowles MR, Zariwala MA
Human mutation34(10)1352-6(2013 Oct)

Mutations in CCDC39 and CCDC40 are the major cause of primary ciliary dyskinesia with axonemal disorganization and absent inner dynein arms.
Antony D, Becker-Heck A, Zariwala MA, Schmidts M, Onoufriadis A, Forouhan M, Wilson R, Taylor-Cox T, Dewar A, Jackson C, Goggin P, Loges NT, Olbrich H, Jaspers M, Jorissen M, Leigh MW, Wolf WE, Daniels ML, Noone PG, Ferkol TW, Sagel SD, Rosenfeld M, Rutman A, Dixit A, O'Callaghan C, Lucas JS, Hogg C, Scambler PJ, Emes RD, , Chung EM, Shoemark A, Knowles MR, Omran H, Mitchison HM
Human mutation34(3)462-72(2013 Mar)

Mutations in radial spoke head genes and ultrastructural cilia defects in East-European cohort of primary ciliary dyskinesia patients.
Zi?tkiewicz E, Bukowy-Biery??o Z, Voelkel K, Klimek B, Dme?ska H, Pogorzelski A, Sulikowska-Rowi?ska A, Rutkiewicz E, Witt M
PloS one7(3)e33667(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Absence of RSPH4A due to mutations in RSPH4A results in deficient axonemal assembly of the RS head components RSPH1 and RSPH9.
Title: Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.

the c.921+3_6delAAGT splice site mutation in RSPH4A is a founder mutation that is a common cause of PCD without situs abnormalities in patients of Hispanic Puerto Rican descent.
Title: Founder mutation in RSPH4A identified in patients of Hispanic descent with primary ciliary dyskinesia.

Mutations in RSPH4A leads to ultrastructural cilia defects and ciliary dyskinesia.
Title: Mutations in radial spoke head genes and ultrastructural cilia defects in East-European cohort of primary ciliary dyskinesia patients.

The following RSPH4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RSPH4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16518	NM_001161664.1 Latest version!	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu19714	NM_001010892.3 Latest version!	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu16518	XM_017010826.1 Latest version!	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu19714	NM_001010892.2	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RSPH4A ( NM_001161664.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001155136.1 Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16518
Clone ID Related Accession (Same CDS sequence)	XM_017010826.1 , NM_001161664.1
Accession Version	NM_001161664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	radial spoke head protein 4 homolog A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299754.1, BC106949.2 and CB851663.1. Transcript Variant: This variant (2) is missing an exon in the 3' coding region compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC106949.2, SRR1803611.4980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145240, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGAGATTCAG AATATACCCC 
CCTGGACAAC ACGGTTATCC TCAAATCTCA TTCCACAATA TGCTATTGCA GTCCTTCAAT 
CCAACCTTTG GCCTGGAGCA TATGCCTTCT CCAATGGCAA AAAGTTTGAA AATTTCTACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155136.1
CDS	146..1948
Translation	MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQRFRIYPPGQHGYPQISFHNMLLQSFNPTFGLEHMPSPMAKSLKIST

Target ORF information:

RefSeq Version	NM_001161664.1
Organism	Homo sapiens(human)
Definition	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161664.1

ORF Insert Sequence:

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGAGATTCAG AATATACCCC 
CCTGGACAAC ACGGTTATCC TCAAATCTCA TTCCACAATA TGCTATTGCA GTCCTTCAAT 
CCAACCTTTG GCCTGGAGCA TATGCCTTCT CCAATGGCAA AAAGTTTGAA AATTTCTACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSPH4A ( NM_001010892.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001010892.1 Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19714
Clone ID Related Accession (Same CDS sequence)	NM_001010892.3 , NM_001010892.2
Accession Version	NM_001010892.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	radial spoke head protein 4 homolog A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299754.1, AK095293.1 and CB851663.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001010892.2. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK299754.1, SRR1803613.165504.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000229554.10/ ENSP00000229554.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGGGTCGCTG TAATTGGTTC 
AACTCCATAC AAAAAAATGA GGAAGAAGAA GAGGAAGAAG ATGAAGAAAA AGACGATTCT 
GACTACATAG AACAGGAAGT GGGGCTTCCT CTTTTGACAC CAATCTCTGA AGATTTAGAG 
ATTCAGAATA TACCCCCCTG GACAACACGG TTATCCTCAA ATCTCATTCC ACAATATGCT 
ATTGCAGTCC TTCAATCCAA CCTTTGGCCT GGAGCATATG CCTTCTCCAA TGGCAAAAAG 
TTTGAAAATT TCTACATAGG CTGGGGTCAT AAGTATAGTC CAGACAATTA TACACCCCCA 
GTTCCACCAC CAGTTTATCA AGAATACCCC AGTGGACCAG AAATTACAGA AATGGATGAT 
CCTAGTGTGG AGGAGGAGCA GGCTTTCAGG GCTGCACAAG AAGCAGTTCT ACTCGCAGCT 
GAGAATGAAG AATCTGAGGA AGATGAAGAT GAGGAAGATG ATTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010892.1
CDS	146..2296
Translation	MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQGRCNWFNSIQKNEEEEEEEDEEKDDSDYIEQEVGLPLLTPISEDLEIQNIPPWTTRLSSNLIPQYAIAVLQSNLWPGAYAFSNGKKFENFYIGWGHKYSPDNYTPPVPPPVYQEYPSGPEITEMDDPSVEEEQAFRAAQEAVLLAAENEESEEDEDEEDDYD

Target ORF information:

RefSeq Version	NM_001010892.3
Organism	Homo sapiens(human)
Definition	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010892.3

ORF Insert Sequence:

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGGGTCGCTG TAATTGGTTC 
AACTCCATAC AAAAAAATGA GGAAGAAGAA GAGGAAGAAG ATGAAGAAAA AGACGATTCT 
GACTACATAG AACAGGAAGT GGGGCTTCCT CTTTTGACAC CAATCTCTGA AGATTTAGAG 
ATTCAGAATA TACCCCCCTG GACAACACGG TTATCCTCAA ATCTCATTCC ACAATATGCT 
ATTGCAGTCC TTCAATCCAA CCTTTGGCCT GGAGCATATG CCTTCTCCAA TGGCAAAAAG 
TTTGAAAATT TCTACATAGG CTGGGGTCAT AAGTATAGTC CAGACAATTA TACACCCCCA 
GTTCCACCAC CAGTTTATCA AGAATACCCC AGTGGACCAG AAATTACAGA AATGGATGAT 
CCTAGTGTGG AGGAGGAGCA GGCTTTCAGG GCTGCACAAG AAGCAGTTCT ACTCGCAGCT 
GAGAATGAAG AATCTGAGGA AGATGAAGAT GAGGAAGATG ATTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSPH4A ( NM_001010892.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001010892.1 Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.

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CloneID	OHu19714
Clone ID Related Accession (Same CDS sequence)	NM_001010892.3 , NM_001010892.2
Accession Version	NM_001010892.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	radial spoke head protein 4 homolog A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299754.1, AK095293.1 and CB851663.1. This sequence is a reference standard in the RefSeqGene project. On Jun 9, 2009 this sequence version replaced NM_001010892.1. Transcript Variant: This variant (1) encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: AK299754.1, BC022879.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGGGTCGCTG TAATTGGTTC 
AACTCCATAC AAAAAAATGA GGAAGAAGAA GAGGAAGAAG ATGAAGAAAA AGACGATTCT 
GACTACATAG AACAGGAAGT GGGGCTTCCT CTTTTGACAC CAATCTCTGA AGATTTAGAG 
ATTCAGAATA TACCCCCCTG GACAACACGG TTATCCTCAA ATCTCATTCC ACAATATGCT 
ATTGCAGTCC TTCAATCCAA CCTTTGGCCT GGAGCATATG CCTTCTCCAA TGGCAAAAAG 
TTTGAAAATT TCTACATAGG CTGGGGTCAT AAGTATAGTC CAGACAATTA TACACCCCCA 
GTTCCACCAC CAGTTTATCA AGAATACCCC AGTGGACCAG AAATTACAGA AATGGATGAT 
CCTAGTGTGG AGGAGGAGCA GGCTTTCAGG GCTGCACAAG AAGCAGTTCT ACTCGCAGCT 
GAGAATGAAG AATCTGAGGA AGATGAAGAT GAGGAAGATG ATTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001010892.1
CDS	146..2296
Translation	MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQGRCNWFNSIQKNEEEEEEEDEEKDDSDYIEQEVGLPLLTPISEDLEIQNIPPWTTRLSSNLIPQYAIAVLQSNLWPGAYAFSNGKKFENFYIGWGHKYSPDNYTPPVPPPVYQEYPSGPEITEMDDPSVEEEQAFRAAQEAVLLAAENEESEEDEDEEDDYD

Target ORF information:

RefSeq Version	NM_001010892.2
Organism	Homo sapiens(human)
Definition	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001010892.2

ORF Insert Sequence:

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGGGTCGCTG TAATTGGTTC 
AACTCCATAC AAAAAAATGA GGAAGAAGAA GAGGAAGAAG ATGAAGAAAA AGACGATTCT 
GACTACATAG AACAGGAAGT GGGGCTTCCT CTTTTGACAC CAATCTCTGA AGATTTAGAG 
ATTCAGAATA TACCCCCCTG GACAACACGG TTATCCTCAA ATCTCATTCC ACAATATGCT 
ATTGCAGTCC TTCAATCCAA CCTTTGGCCT GGAGCATATG CCTTCTCCAA TGGCAAAAAG 
TTTGAAAATT TCTACATAGG CTGGGGTCAT AAGTATAGTC CAGACAATTA TACACCCCCA 
GTTCCACCAC CAGTTTATCA AGAATACCCC AGTGGACCAG AAATTACAGA AATGGATGAT 
CCTAGTGTGG AGGAGGAGCA GGCTTTCAGG GCTGCACAAG AAGCAGTTCT ACTCGCAGCT 
GAGAATGAAG AATCTGAGGA AGATGAAGAT GAGGAAGATG ATTATGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RSPH4A ( XM_017010826.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866315.1 Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant X1, mRNA.

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CloneID	OHu16518
Clone ID Related Accession (Same CDS sequence)	XM_017010826.1 , NM_001161664.1
Accession Version	XM_017010826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	radial spoke head protein 4 homolog A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGAGATTCAG AATATACCCC 
CCTGGACAAC ACGGTTATCC TCAAATCTCA TTCCACAATA TGCTATTGCA GTCCTTCAAT 
CCAACCTTTG GCCTGGAGCA TATGCCTTCT CCAATGGCAA AAAGTTTGAA AATTTCTACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866315.1
CDS	3713..5515
Translation	MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQRFRIYPPGQHGYPQISFHNMLLQSFNPTFGLEHMPSPMAKSLKIST

Target ORF information:

RefSeq Version	XM_017010826.1
Organism	Homo sapiens(human)
Definition	Homo sapiens radial spoke head component 4A (RSPH4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010826.1

ORF Insert Sequence:

ATGGAGGACT CAACCTCCCC GAAGCAAGAA AAAGAAAACC AAGAAGAACT AGGGGAAACA 
AGGCGGCCAT GGGAAGGAAA GACAGCAGCT TCTCCCCAAT ATTCTGAGCC TGAGTCGTCT 
GAGCCCTTGG AGGCGAAGCA GGGGCCAGAA ACTGGACGCC AGTCCCGAAG CAGCCGTCCT 
TGGAGCCCGC AGTCTAGAGC CAAGACGCCT CTGGGTGGCC CCGCGGGACC AGAAACATCA 
TCACCTGCTC CTGTCTCTCC GCGGGAGCCC TCTTCCTCTC CTTCTCCCCT GGCTCCGGCC 
AGACAAGACC TCGCGGCACC ACCTCAGTCG GACAGGACCA CGAGTGTGAT TCCTGAAGCT 
GGGACACCTT ATCCTGATCC TTTGGAACAA TCATCTGATA AAAGAGAATC AACTCCTCAT 
CACACAAGCC AGTCAGAAGG AAACACCTTT CAACAGTCTC AGCAACCCAA ACCCCACCTG 
TGTGGACGAA GGGACGTGAG CTATAACAAC GCTAAACAGA AAGAGCTGAG ATTTGACGTT 
TTTCAGGAGG AAGACTCAAA CAGTGACTAT GATTTACAGC AGCCGGCGCC TGGGGGCTCT 
GAAGTGGCCC CCAGCATGCT TGAGATCACC ATTCAGAATG CTAAGGCTTA CCTGCTGAAG 
ACTAGCAGCA ATTCGGGCTT TAATCTATAT GATCATCTTT CTAATATGTT GACCAAGATA 
TTAAATGAGC GTCCTGAAAA TGCTGTTGAC ATCTTTGAAA ATATTAGCCA AGATGTGAAG 
ATGGCACATT TTAGTAAAAA ATTTGATGCA CTACAAAATG AGAATGAGTT GCTTCCAACA 
TATGAAATAG CAGAAAAGCA AAAGGCTCTT TTTCTCCAGG GACATTTGGA AGGAGTTGAC 
CAAGAATTGG AAGATGAAAT AGCAGAAAAC GCTCTTCCAA ATGTAATGGA GTCAGCTTTT 
TATTTTGAAC AAGCTGGAGT TGGTTTGGGC ACAGATGAGA CATACCGCAT ATTTCTTGCC 
CTCAAGCAGC TTACTGATAC CCACCCAATC CAAAGATGCC GCTTCTGGGG AAAGATCTTG 
GGTCTGGAAA TGAATTATAT TGTAGCTGAA GTGGAATTTC GTGAGGGGGA AGATGAAGAG 
GAAGTGGAAG AGGAAGATGT AGCTGAAGAG AGGGACAATG GAGAAAGTGA AGCTCATGAA 
GATGAGGAAG ATGAATTACC AAAGTCCTTT TACAAGGCCC CACAGGCTAT ACCAAAAGAA 
GAAAGTAGAA CAGGTGCCAA CAAATATGTC TATTTTGTTT GCAATGAACC AGGAAGACCA 
TGGGTGAAGT TACCACCAGT TATACCTGCA CAAATTGTTA TTGCAAGAAA AATCAAGAAA 
TTTTTCACTG GGCGATTGGA TGCTCCCATC ATAAGCTACC CACCTTTCCC AGGAAATGAG 
AGTAATTATT TACGAGCACA AATTGCCCGA ATTTCAGCAG GAACCCACGT CAGTCCTCTA 
GGATTTTATC AGTTTGGTGA AGAGGAAGGA GAGGAGGAGG AAGAGGCAGA AGGTGGGCGA 
AATAGCTTTG AGGAAAACCC TGATTTTGAA GGCATCCAAG TGATTGATCT AGTAGAATCC 
CTATCCAATT GGGTTCATCA TGTACAGCAT ATTCTCTCTC AGAGATTCAG AATATACCCC 
CCTGGACAAC ACGGTTATCC TCAAATCTCA TTCCACAATA TGCTATTGCA GTCCTTCAAT 
CCAACCTTTG GCCTGGAGCA TATGCCTTCT CCAATGGCAA AAAGTTTGAA AATTTCTACA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A targeted next-generation sequencing panel reveals novel mutations in Japanese patients with primary ciliary dyskinesia.
Auris, nasus, larynx(2017 Sep)
Takeuchi K,Kitano M,Kiyotoshi H,Ikegami K,Ogawa S,Ikejiri M,Nagao M,Fujisawa T,Nakatani K

Immunofluorescence Analysis and Diagnosis of Primary Ciliary Dyskinesia with Radial Spoke Defects.
American journal of respiratory cell and molecular biology53(4)563-73(2015 Oct)
Frommer A,Hjeij R,Loges NT,Edelbusch C,Jahnke C,Raidt J,Werner C,Wallmeier J,Gro?e-Onnebrink J,Olbrich H,Cindri? S,Jaspers M,Boon M,Memari Y,Durbin R,Kolb-Kokocinski A,Sauer S,Marthin JK,Nielsen KG,Amirav I,Elias N,Kerem E,Shoseyov D,Haeffner K,Omran H

Founder mutation in RSPH4A identified in patients of Hispanic descent with primary ciliary dyskinesia.
Human mutation34(10)1352-6(2013 Oct)
Daniels ML,Leigh MW,Davis SD,Armstrong MC,Carson JL,Hazucha M,Dell SD,Eriksson M,Collins FS,Knowles MR,Zariwala MA

Mutations in CCDC39 and CCDC40 are the major cause of primary ciliary dyskinesia with axonemal disorganization and absent inner dynein arms.
Human mutation34(3)462-72(2013 Mar)
Antony D,Becker-Heck A,Zariwala MA,Schmidts M,Onoufriadis A,Forouhan M,Wilson R,Taylor-Cox T,Dewar A,Jackson C,Goggin P,Loges NT,Olbrich H,Jaspers M,Jorissen M,Leigh MW,Wolf WE,Daniels ML,Noone PG,Ferkol TW,Sagel SD,Rosenfeld M,Rutman A,Dixit A,O'Callaghan C,Lucas JS,Hogg C,Scambler PJ,Emes RD,,Chung EM,Shoemark A,Knowles MR,Omran H,Mitchison HM

Mutations in radial spoke head genes and ultrastructural cilia defects in East-European cohort of primary ciliary dyskinesia patients.
PloS one7(3)e33667(2012)
Zi?tkiewicz E,Bukowy-Biery??o Z,Voelkel K,Klimek B,Dme?ska H,Pogorzelski A,Sulikowska-Rowi?ska A,Rutkiewicz E,Witt M

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RSPH4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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