HEXB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HEXB
Entrez Gene ID	3074
Full Name	hexosaminidase subunit beta
Synonyms	ENC-1AS,HEL-248,HEL-S-111
General protein information	Preferred Names hexosaminidase subunit beta Names beta-hexosaminidase subunit beta HCC-7 N-acetyl-beta-glucosaminidase subunit beta beta-N-acetylhexosaminidase subunit beta beta-hexosaminidase beta-subunit cervical cancer proto-oncogene 7 protein epididymis luminal protein 248 epididymis secretory protein Li 111 hexosaminidase B (beta polypeptide) hexosaminidase subunit B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.3
Summary	Hexosaminidase B is the beta subunit of the lysosomal enzyme beta-hexosaminidase that, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Beta-hexosaminidase is composed of two subunits, alpha and beta, which are encoded by separate genes. Both beta-hexosaminidase alpha and beta subunits are members of family 20 of glycosyl hydrolases. Mutations in the alpha or beta subunit genes lead to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. Beta subunit gene mutations lead to Sandhoff disease (GM2-gangliosidosis type II). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014].
Disorder MIM:	606873
Disorder Html:	Sandhoff disease, infantile, juvenile, and adult forms, 268800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000521.3	NP_000512.1	beta-hexosaminidase subunit beta isoform 1 preproprotein
NM_000521.4	NP_000512.2	beta-hexosaminidase subunit beta isoform 1 preproprotein
NM_001292004.1	NP_001278933.1	beta-hexosaminidase subunit beta isoform 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HEXB	XP_005617969.1
Gallus gallus (chicken)	HEXB	XP_424791.3
Danio rerio (zebrafish)	hexa	NP_001108317.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010056	XP_319210.4
Xenopus tropicalis (tropical clawed frog)	hexb	NP_001120459.1
Pan troglodytes (chimpanzee)	HEXB	XP_001150632.1
Rattus norvegicus (Norway rat)	Hexb	NP_001011946.1
Oryza sativa (rice)	Os05g0115900	NP_001054468.1
Mus musculus (house mouse)	Hexb	NP_034552.1
	HEXB	XP_001103139.1
Arabidopsis thaliana (thale cress)	HEXO1	NP_567017.2
Homo sapiens (human)	HEXB	NP_000512.1
Bos taurus (cattle)	HEXB	NP_001069978.2
Bos taurus (cattle)	LOC786974	XP_002696308.3
Caenorhabditis elegans (roundworm)	hex-1	NP_508409.1

Gene Ontology
Bibliography

Related articles in PubMed

Pediatric reference data on activity of urinary N-acetyl-?-D-hexosaminidase and its isoenzymes.
Zalewska-Szajda B, Taranta-Janusz K, Chojnowska S, Waszkiewicz N, Zwierz K, Wasilewska A
Advances in medical sciences63(1)94-99(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Co-existence of phenylketonuria either with maple syrup urine disease or Sandhoff disease in two patients from : emphasizing the role of consanguinity.
Abiri M, Talebi S, Uitto J, Youssefian L, Vahidnezhad H, Shirzad T, Salehpour S, Zeinali S
Journal of pediatric endocrinology & metabolism : JPEM29(10)1215-1219(2016 Oct)

Protease-resistant modified human ?-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.
Kitakaze K, Mizutani Y, Sugiyama E, Tasaki C, Tsuji D, Maita N, Hirokawa T, Asanuma D, Kamiya M, Sato K, Setou M, Urano Y, Togawa T, Otaka A, Sakuraba H, Itoh K
The Journal of clinical investigation126(5)1691-703(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations of the HEXB gene is associated with maple syrup urine disease or Sandhoff disease.
Title: Co-existence of phenylketonuria either with maple syrup urine disease or Sandhoff disease in two patients from : emphasizing the role of consanguinity.

a modified human hexosaminidase subunit beta (HexB), which we have termed mod2B, composed of homodimeric beta subunits that contain amino acid sequences from the alpha subunit that confer GM2 ganglioside-degrading activity and protease resistance.
Title: Protease-resistant modified human ?-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.

report on the heterogeneity of the mutational spectrum of the HEXB gene in Indian patients with Sandhoff disease
Title: Clinical, biochemical and mutation profile in Indian patients with Sandhoff disease.

A total of 19 HEXB variants were found in the 1092 genomes of which 5 are suspected of having a deleterious effect on hexosaminidase activity.
Title: Incidence and carrier frequency of Sandhoff disease in Saskatchewan determined using a novel substrate with detection by tandem mass spectrometry and molecular genetic analysis.

DNA from ian Tay-Sachs patients reveals a novel mutation in HEXB predicting a termination codon or nonsense mutation.
Title: Three novel mutations in ian patients with Tay-Sachs disease.

The following HEXB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HEXB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25080	NM_000521.3 Latest version!	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu25266	NM_001292004.1 Latest version!	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu107849	NM_000521.4 Latest version!	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HEXB ( NM_000521.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000512.1 Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25080
Clone ID Related Accession (Same CDS sequence)	NM_000521.3
Accession Version	NM_000521.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit beta isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK130002.1 and BC017378.2. This sequence is a reference standard in the RefSeqGene project. On May 15, 2008 this sequence version replaced NM_000521.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130002.1, SRR1163657.411191.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCTGT GCGGGCTGGG GCTGCCCCGG CCGCCCATGC TGCTGGCGCT GCTGTTGGCG 
ACACTGCTGG CGGCGATGTT GGCGCTGCTG ACTCAGGTGG CGCTGGTGGT GCAGGTGGCG 
GAGGCGGCTC GGGCCCCGAG CGTCTCGGCC AAGCCGGGGC CGGCGCTGTG GCCCCTGCCG 
CTCTCGGTGA AGATGACCCC GAACCTGCTG CATCTCGCCC CGGAGAACTT CTACATCAGC 
CACAGCCCCA ATTCCACGGC GGGCCCCTCC TGCACCCTGC TGGAGGAAGC GTTTCGACGA 
TATCATGGCT ATATTTTTGG TTTCTACAAG TGGCATCATG AACCTGCTGA ATTCCAGGCT 
AAAACCCAGG TTCAGCAACT TCTTGTCTCA ATCACCCTTC AGTCAGAGTG TGATGCTTTC 
CCCAACATAT CTTCAGATGA GTCTTATACT TTACTTGTGA AAGAACCAGT GGCTGTCCTT 
AAGGCCAACA GAGTTTGGGG AGCATTACGA GGTTTAGAGA CCTTTAGCCA GTTAGTTTAT 
CAAGATTCTT ATGGAACTTT CACCATCAAT GAATCCACCA TTATTGATTC TCCAAGGTTT 
TCTCACAGAG GAATTTTGAT TGATACATCC AGACATTATC TGCCAGTTAA GATTATTCTT 
AAAACTCTGG ATGCCATGGC TTTTAATAAG TTTAATGTTC TTCACTGGCA CATAGTTGAT 
GACCAGTCTT TCCCATATCA GAGCATCACT TTTCCTGAGT TAAGCAATAA AGGAAGCTAT 
TCTTTGTCTC ATGTTTATAC ACCAAATGAT GTCCGTATGG TGATTGAATA TGCCAGATTA 
CGAGGAATTC GAGTCCTGCC AGAATTTGAT ACCCCTGGGC ATACACTATC TTGGGGAAAA 
GGTCAGAAAG ACCTCCTGAC TCCATGTTAC AGTAGACAAA ACAAGTTGGA CTCTTTTGGA 
CCTATAAACC CTACTCTGAA TACAACATAC AGCTTCCTTA CTACATTTTT CAAAGAAATT 
AGTGAGGTGT TTCCAGATCA ATTCATTCAT TTGGGAGGAG ATGAAGTGGA ATTTAAATGT 
TGGGAATCAA ATCCAAAAAT TCAAGATTTC ATGAGGCAAA AAGGCTTTGG CACAGATTTT 
AAGAAACTAG AATCTTTCTA CATTCAAAAG GTTTTGGATA TTATTGCAAC CATAAACAAG 
GGATCCATTG TCTGGCAGGA GGTTTTTGAT GATAAAGCAA AGCTTGCGCC GGGCACAATA 
GTTGAAGTAT GGAAAGACAG CGCATATCCT GAGGAACTCA GTAGAGTCAC AGCATCTGGC 
TTCCCTGTAA TCCTTTCTGC TCCTTGGTAC TTAGATTTGA TTAGCTATGG ACAAGATTGG 
AGGAAATACT ATAAAGTGGA ACCTCTTGAT TTTGGCGGTA CTCAGAAACA GAAACAACTT 
TTCATTGGTG GAGAAGCTTG TCTATGGGGA GAATATGTGG ATGCAACTAA CCTCACTCCA 
AGATTATGGC CTCGGGCAAG TGCTGTTGGT GAGAGACTCT GGAGTTCCAA AGATGTCAGA 
GATATGGATG ACGCCTATGA CAGACTGACA AGGCACCGCT GCAGGATGGT CGAACGTGGA 
ATAGCTGCAC AACCTCTTTA TGCTGGATAT TGTAACCATG AGAACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000512.1
CDS	118..1788
Translation	MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLSVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

Target ORF information:

RefSeq Version	NM_000521.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000521.3

ORF Insert Sequence:

ATGGAGCTGT GCGGGCTGGG GCTGCCCCGG CCGCCCATGC TGCTGGCGCT GCTGTTGGCG 
ACACTGCTGG CGGCGATGTT GGCGCTGCTG ACTCAGGTGG CGCTGGTGGT GCAGGTGGCG 
GAGGCGGCTC GGGCCCCGAG CGTCTCGGCC AAGCCGGGGC CGGCGCTGTG GCCCCTGCCG 
CTCTCGGTGA AGATGACCCC GAACCTGCTG CATCTCGCCC CGGAGAACTT CTACATCAGC 
CACAGCCCCA ATTCCACGGC GGGCCCCTCC TGCACCCTGC TGGAGGAAGC GTTTCGACGA 
TATCATGGCT ATATTTTTGG TTTCTACAAG TGGCATCATG AACCTGCTGA ATTCCAGGCT 
AAAACCCAGG TTCAGCAACT TCTTGTCTCA ATCACCCTTC AGTCAGAGTG TGATGCTTTC 
CCCAACATAT CTTCAGATGA GTCTTATACT TTACTTGTGA AAGAACCAGT GGCTGTCCTT 
AAGGCCAACA GAGTTTGGGG AGCATTACGA GGTTTAGAGA CCTTTAGCCA GTTAGTTTAT 
CAAGATTCTT ATGGAACTTT CACCATCAAT GAATCCACCA TTATTGATTC TCCAAGGTTT 
TCTCACAGAG GAATTTTGAT TGATACATCC AGACATTATC TGCCAGTTAA GATTATTCTT 
AAAACTCTGG ATGCCATGGC TTTTAATAAG TTTAATGTTC TTCACTGGCA CATAGTTGAT 
GACCAGTCTT TCCCATATCA GAGCATCACT TTTCCTGAGT TAAGCAATAA AGGAAGCTAT 
TCTTTGTCTC ATGTTTATAC ACCAAATGAT GTCCGTATGG TGATTGAATA TGCCAGATTA 
CGAGGAATTC GAGTCCTGCC AGAATTTGAT ACCCCTGGGC ATACACTATC TTGGGGAAAA 
GGTCAGAAAG ACCTCCTGAC TCCATGTTAC AGTAGACAAA ACAAGTTGGA CTCTTTTGGA 
CCTATAAACC CTACTCTGAA TACAACATAC AGCTTCCTTA CTACATTTTT CAAAGAAATT 
AGTGAGGTGT TTCCAGATCA ATTCATTCAT TTGGGAGGAG ATGAAGTGGA ATTTAAATGT 
TGGGAATCAA ATCCAAAAAT TCAAGATTTC ATGAGGCAAA AAGGCTTTGG CACAGATTTT 
AAGAAACTAG AATCTTTCTA CATTCAAAAG GTTTTGGATA TTATTGCAAC CATAAACAAG 
GGATCCATTG TCTGGCAGGA GGTTTTTGAT GATAAAGCAA AGCTTGCGCC GGGCACAATA 
GTTGAAGTAT GGAAAGACAG CGCATATCCT GAGGAACTCA GTAGAGTCAC AGCATCTGGC 
TTCCCTGTAA TCCTTTCTGC TCCTTGGTAC TTAGATTTGA TTAGCTATGG ACAAGATTGG 
AGGAAATACT ATAAAGTGGA ACCTCTTGAT TTTGGCGGTA CTCAGAAACA GAAACAACTT 
TTCATTGGTG GAGAAGCTTG TCTATGGGGA GAATATGTGG ATGCAACTAA CCTCACTCCA 
AGATTATGGC CTCGGGCAAG TGCTGTTGGT GAGAGACTCT GGAGTTCCAA AGATGTCAGA 
GATATGGATG ACGCCTATGA CAGACTGACA AGGCACCGCT GCAGGATGGT CGAACGTGGA 
ATAGCTGCAC AACCTCTTTA TGCTGGATAT TGTAACCATG AGAACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXB ( NM_001292004.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278933.1 Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25266
Clone ID Related Accession (Same CDS sequence)	NM_001292004.1
Accession Version	NM_001292004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit beta isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY643499.1 and BC017378.2. Transcript Variant: This variant (2) contains an alternate 5' terminal exon, which causes translation initiation at a downstream AUG start codon, compared to variant 1. The resulting isoform (2) has a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY643499.1, SRR1660807.139842.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END##

ATGGCTTTTA ATAAGTTTAA TGTTCTTCAC TGGCACATAG TTGATGACCA GTCTTTCCCA 
TATCAGAGCA TCACTTTTCC TGAGTTAAGC AATAAAGGAA GCTATTCTTT GTCTCATGTT 
TATACACCAA ATGATGTCCG TATGGTGATT GAATATGCCA GATTACGAGG AATTCGAGTC 
CTGCCAGAAT TTGATACCCC TGGGCATACA CTATCTTGGG GAAAAGGTCA GAAAGACCTC 
CTGACTCCAT GTTACAGTAG ACAAAACAAG TTGGACTCTT TTGGACCTAT AAACCCTACT 
CTGAATACAA CATACAGCTT CCTTACTACA TTTTTCAAAG AAATTAGTGA GGTGTTTCCA 
GATCAATTCA TTCATTTGGG AGGAGATGAA GTGGAATTTA AATGTTGGGA ATCAAATCCA 
AAAATTCAAG ATTTCATGAG GCAAAAAGGC TTTGGCACAG ATTTTAAGAA ACTAGAATCT 
TTCTACATTC AAAAGGTTTT GGATATTATT GCAACCATAA ACAAGGGATC CATTGTCTGG 
CAGGAGGTTT TTGATGATAA AGCAAAGCTT GCGCCGGGCA CAATAGTTGA AGTATGGAAA 
GACAGCGCAT ATCCTGAGGA ACTCAGTAGA GTCACAGCAT CTGGCTTCCC TGTAATCCTT 
TCTGCTCCTT GGTACTTAGA TTTGATTAGC TATGGACAAG ATTGGAGGAA ATACTATAAA 
GTGGAACCTC TTGATTTTGG CGGTACTCAG AAACAGAAAC AACTTTTCAT TGGTGGAGAA 
GCTTGTCTAT GGGGAGAATA TGTGGATGCA ACTAACCTCA CTCCAAGATT ATGGCCTCGG 
GCAAGTGCTG TTGGTGAGAG ACTCTGGAGT TCCAAAGATG TCAGAGATAT GGATGACGCC 
TATGACAGAC TGACAAGGCA CCGCTGCAGG ATGGTCGAAC GTGGAATAGC TGCACAACCT 
CTTTATGCTG GATATTGTAA CCATGAGAAC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278933.1
CDS	913..1908
Translation	MAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

Target ORF information:

RefSeq Version	NM_001292004.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292004.1

ORF Insert Sequence:

ATGGCTTTTA ATAAGTTTAA TGTTCTTCAC TGGCACATAG TTGATGACCA GTCTTTCCCA 
TATCAGAGCA TCACTTTTCC TGAGTTAAGC AATAAAGGAA GCTATTCTTT GTCTCATGTT 
TATACACCAA ATGATGTCCG TATGGTGATT GAATATGCCA GATTACGAGG AATTCGAGTC 
CTGCCAGAAT TTGATACCCC TGGGCATACA CTATCTTGGG GAAAAGGTCA GAAAGACCTC 
CTGACTCCAT GTTACAGTAG ACAAAACAAG TTGGACTCTT TTGGACCTAT AAACCCTACT 
CTGAATACAA CATACAGCTT CCTTACTACA TTTTTCAAAG AAATTAGTGA GGTGTTTCCA 
GATCAATTCA TTCATTTGGG AGGAGATGAA GTGGAATTTA AATGTTGGGA ATCAAATCCA 
AAAATTCAAG ATTTCATGAG GCAAAAAGGC TTTGGCACAG ATTTTAAGAA ACTAGAATCT 
TTCTACATTC AAAAGGTTTT GGATATTATT GCAACCATAA ACAAGGGATC CATTGTCTGG 
CAGGAGGTTT TTGATGATAA AGCAAAGCTT GCGCCGGGCA CAATAGTTGA AGTATGGAAA 
GACAGCGCAT ATCCTGAGGA ACTCAGTAGA GTCACAGCAT CTGGCTTCCC TGTAATCCTT 
TCTGCTCCTT GGTACTTAGA TTTGATTAGC TATGGACAAG ATTGGAGGAA ATACTATAAA 
GTGGAACCTC TTGATTTTGG CGGTACTCAG AAACAGAAAC AACTTTTCAT TGGTGGAGAA 
GCTTGTCTAT GGGGAGAATA TGTGGATGCA ACTAACCTCA CTCCAAGATT ATGGCCTCGG 
GCAAGTGCTG TTGGTGAGAG ACTCTGGAGT TCCAAAGATG TCAGAGATAT GGATGACGCC 
TATGACAGAC TGACAAGGCA CCGCTGCAGG ATGGTCGAAC GTGGAATAGC TGCACAACCT 
CTTTATGCTG GATATTGTAA CCATGAGAAC ATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HEXB ( NM_000521.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000512.2 Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107849
Clone ID Related Accession (Same CDS sequence)	NM_000521.4
Accession Version	NM_000521.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1671bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	beta-hexosaminidase subunit beta isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC026405.5 and AC093214.2. On Nov 22, 2018 this sequence version replaced NM_000521.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF378118.1, SRR3476690.59226.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000261416.12/ ENSP00000261416.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTGT GCGGGCTGGG GCTGCCCCGG CCGCCCATGC TGCTGGCGCT GCTGTTGGCG 
ACACTGCTGG CGGCGATGTT GGCGCTGCTG ACTCAGGTGG CGCTGGTGGT GCAGGTGGCG 
GAGGCGGCTC GGGCCCCGAG CGTCTCGGCC AAGCCGGGGC CGGCGCTGTG GCCCCTGCCG 
CTCTTGGTGA AGATGACCCC GAACCTGCTG CATCTCGCCC CGGAGAACTT CTACATCAGC 
CACAGCCCCA ATTCCACGGC GGGCCCCTCC TGCACCCTGC TGGAGGAAGC GTTTCGACGA 
TATCATGGCT ATATTTTTGG TTTCTACAAG TGGCATCATG AACCTGCTGA ATTCCAGGCT 
AAAACCCAGG TTCAGCAACT TCTTGTCTCA ATCACCCTTC AGTCAGAGTG TGATGCTTTC 
CCCAACATAT CTTCAGATGA GTCTTATACT TTACTTGTGA AAGAACCAGT GGCTGTCCTT 
AAGGCCAACA GAGTTTGGGG AGCATTACGA GGTTTAGAGA CCTTTAGCCA GTTAGTTTAT 
CAAGATTCTT ATGGAACTTT CACCATCAAT GAATCCACCA TTATTGATTC TCCAAGGTTT 
TCTCACAGAG GAATTTTGAT TGATACATCC AGACATTATC TGCCAGTTAA GATTATTCTT 
AAAACTCTGG ATGCCATGGC TTTTAATAAG TTTAATGTTC TTCACTGGCA CATAGTTGAT 
GACCAGTCTT TCCCATATCA GAGCATCACT TTTCCTGAGT TAAGCAATAA AGGAAGCTAT 
TCTTTGTCTC ATGTTTATAC ACCAAATGAT GTCCGTATGG TGATTGAATA TGCCAGATTA 
CGAGGAATTC GAGTCCTGCC AGAATTTGAT ACCCCTGGGC ATACACTATC TTGGGGAAAA 
GGTCAGAAAG ACCTCCTGAC TCCATGTTAC AGTAGACAAA ACAAGTTGGA CTCTTTTGGA 
CCTATAAACC CTACTCTGAA TACAACATAC AGCTTCCTTA CTACATTTTT CAAAGAAATT 
AGTGAGGTGT TTCCAGATCA ATTCATTCAT TTGGGAGGAG ATGAAGTGGA ATTTAAATGT 
TGGGAATCAA ATCCAAAAAT TCAAGATTTC ATGAGGCAAA AAGGCTTTGG CACAGATTTT 
AAGAAACTAG AATCTTTCTA CATTCAAAAG GTTTTGGATA TTATTGCAAC CATAAACAAG 
GGATCCATTG TCTGGCAGGA GGTTTTTGAT GATAAAGCAA AGCTTGCGCC GGGCACAATA 
GTTGAAGTAT GGAAAGACAG CGCATATCCT GAGGAACTCA GTAGAGTCAC AGCATCTGGC 
TTCCCTGTAA TCCTTTCTGC TCCTTGGTAC TTAGATTTGA TTAGCTATGG ACAAGATTGG 
AGGAAATACT ATAAAGTGGA ACCTCTTGAT TTTGGCGGTA CTCAGAAACA GAAACAACTT 
TTCATTGGTG GAGAAGCTTG TCTATGGGGA GAATATGTGG ATGCAACTAA CCTCACTCCA 
AGATTATGGC CTCGGGCAAG TGCTGTTGGT GAGAGACTCT GGAGTTCCAA AGATGTCAGA 
GATATGGATG ACGCCTATGA CAGACTGACA AGGCACCGCT GCAGGATGGT CGAACGTGGA 
ATAGCTGCAC AACCTCTTTA TGCTGGATAT TGTAACCATG AGAACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000512.2
CDS	29..1699
Translation	MELCGLGLPRPPMLLALLLATLLAAMLALLTQVALVVQVAEAARAPSVSAKPGPALWPLPLLVKMTPNLLHLAPENFYISHSPNSTAGPSCTLLEEAFRRYHGYIFGFYKWHHEPAEFQAKTQVQQLLVSITLQSECDAFPNISSDESYTLLVKEPVAVLKANRVWGALRGLETFSQLVYQDSYGTFTINESTIIDSPRFSHRGILIDTSRHYLPVKIILKTLDAMAFNKFNVLHWHIVDDQSFPYQSITFPELSNKGSYSLSHVYTPNDVRMVIEYARLRGIRVLPEFDTPGHTLSWGKGQKDLLTPCYSRQNKLDSFGPINPTLNTTYSFLTTFFKEISEVFPDQFIHLGGDEVEFKCWESNPKIQDFMRQKGFGTDFKKLESFYIQKVLDIIATINKGSIVWQEVFDDKAKLAPGTIVEVWKDSAYPEELSRVTASGFPVILSAPWYLDLISYGQDWRKYYKVEPLDFGGTQKQKQLFIGGEACLWGEYVDATNLTPRLWPRASAVGERLWSSKDVRDMDDAYDRLTRHRCRMVERGIAAQPLYAGYCNHENM

Target ORF information:

RefSeq Version	NM_000521.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hexosaminidase subunit beta (HEXB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000521.4

ORF Insert Sequence:

ATGGAGCTGT GCGGGCTGGG GCTGCCCCGG CCGCCCATGC TGCTGGCGCT GCTGTTGGCG 
ACACTGCTGG CGGCGATGTT GGCGCTGCTG ACTCAGGTGG CGCTGGTGGT GCAGGTGGCG 
GAGGCGGCTC GGGCCCCGAG CGTCTCGGCC AAGCCGGGGC CGGCGCTGTG GCCCCTGCCG 
CTCTTGGTGA AGATGACCCC GAACCTGCTG CATCTCGCCC CGGAGAACTT CTACATCAGC 
CACAGCCCCA ATTCCACGGC GGGCCCCTCC TGCACCCTGC TGGAGGAAGC GTTTCGACGA 
TATCATGGCT ATATTTTTGG TTTCTACAAG TGGCATCATG AACCTGCTGA ATTCCAGGCT 
AAAACCCAGG TTCAGCAACT TCTTGTCTCA ATCACCCTTC AGTCAGAGTG TGATGCTTTC 
CCCAACATAT CTTCAGATGA GTCTTATACT TTACTTGTGA AAGAACCAGT GGCTGTCCTT 
AAGGCCAACA GAGTTTGGGG AGCATTACGA GGTTTAGAGA CCTTTAGCCA GTTAGTTTAT 
CAAGATTCTT ATGGAACTTT CACCATCAAT GAATCCACCA TTATTGATTC TCCAAGGTTT 
TCTCACAGAG GAATTTTGAT TGATACATCC AGACATTATC TGCCAGTTAA GATTATTCTT 
AAAACTCTGG ATGCCATGGC TTTTAATAAG TTTAATGTTC TTCACTGGCA CATAGTTGAT 
GACCAGTCTT TCCCATATCA GAGCATCACT TTTCCTGAGT TAAGCAATAA AGGAAGCTAT 
TCTTTGTCTC ATGTTTATAC ACCAAATGAT GTCCGTATGG TGATTGAATA TGCCAGATTA 
CGAGGAATTC GAGTCCTGCC AGAATTTGAT ACCCCTGGGC ATACACTATC TTGGGGAAAA 
GGTCAGAAAG ACCTCCTGAC TCCATGTTAC AGTAGACAAA ACAAGTTGGA CTCTTTTGGA 
CCTATAAACC CTACTCTGAA TACAACATAC AGCTTCCTTA CTACATTTTT CAAAGAAATT 
AGTGAGGTGT TTCCAGATCA ATTCATTCAT TTGGGAGGAG ATGAAGTGGA ATTTAAATGT 
TGGGAATCAA ATCCAAAAAT TCAAGATTTC ATGAGGCAAA AAGGCTTTGG CACAGATTTT 
AAGAAACTAG AATCTTTCTA CATTCAAAAG GTTTTGGATA TTATTGCAAC CATAAACAAG 
GGATCCATTG TCTGGCAGGA GGTTTTTGAT GATAAAGCAA AGCTTGCGCC GGGCACAATA 
GTTGAAGTAT GGAAAGACAG CGCATATCCT GAGGAACTCA GTAGAGTCAC AGCATCTGGC 
TTCCCTGTAA TCCTTTCTGC TCCTTGGTAC TTAGATTTGA TTAGCTATGG ACAAGATTGG 
AGGAAATACT ATAAAGTGGA ACCTCTTGAT TTTGGCGGTA CTCAGAAACA GAAACAACTT 
TTCATTGGTG GAGAAGCTTG TCTATGGGGA GAATATGTGG ATGCAACTAA CCTCACTCCA 
AGATTATGGC CTCGGGCAAG TGCTGTTGGT GAGAGACTCT GGAGTTCCAA AGATGTCAGA 
GATATGGATG ACGCCTATGA CAGACTGACA AGGCACCGCT GCAGGATGGT CGAACGTGGA 
ATAGCTGCAC AACCTCTTTA TGCTGGATAT TGTAACCATG AGAACATGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pediatric reference data on activity of urinary N-acetyl-?-D-hexosaminidase and its isoenzymes.
Advances in medical sciences63(1)94-99(2017 Aug)
Zalewska-Szajda B,Taranta-Janusz K,Chojnowska S,Waszkiewicz N,Zwierz K,Wasilewska A

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Co-existence of phenylketonuria either with maple syrup urine disease or Sandhoff disease in two patients from Iran: emphasizing the role of consanguinity.
Journal of pediatric endocrinology & metabolism : JPEM29(10)1215-1219(2016 Oct)
Abiri M,Talebi S,Uitto J,Youssefian L,Vahidnezhad H,Shirzad T,Salehpour S,Zeinali S

Protease-resistant modified human ?-hexosaminidase B ameliorates symptoms in GM2 gangliosidosis model.
The Journal of clinical investigation126(5)1691-703(2016 May)
Kitakaze K,Mizutani Y,Sugiyama E,Tasaki C,Tsuji D,Maita N,Hirokawa T,Asanuma D,Kamiya M,Sato K,Setou M,Urano Y,Togawa T,Otaka A,Sakuraba H,Itoh K

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HEXB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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