H3-3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	H3-3B
Entrez Gene ID	3021
Full Name	H3 histone family member 3B
Synonyms	H3.3B
General protein information	Preferred Names H3 histone family member 3B Names histone H3.3 H3 histone, family 3B (H3.3B)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.1
Summary	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family. Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17. [provided by RefSeq, Oct 2015].
Disorder MIM:	601058

mRNA and Protein(s)

mRNA	Protein	Name
NM_005324.4	NP_005315.1	histone H3.3
NM_005324.5	NP_005315.1	histone H3.3

Pathways from BioSystems

Homologies

Homo sapiens (human)	H3F3B	NP_005315.1
Mus musculus (house mouse)	H3f3b	NP_032237.1
Gallus gallus (chicken)	H3F3B	NP_990627.1
Pan troglodytes (chimpanzee)	H3F3B	XP_003315784.1
Pan troglodytes (chimpanzee)	H3F3A	XP_003339055.1
	LOC707040	XP_001105140.1
Rattus norvegicus (Norway rat)	H3f3b	NP_446437.1
Drosophila melanogaster (fruit fly)	His3:CG33857	NP_001027373.1
Homo sapiens (human)	H3F3A	NP_002098.1
Mus musculus (house mouse)	H3f3a	NP_032236.1
Gallus gallus (chicken)	LOC100859629	XP_003643522.1
Xenopus tropicalis (tropical clawed frog)	LOC101730642	XP_004913911.1
Canis lupus familiaris (dog)	H3F3B	XP_537232.1
Bos taurus (cattle)	H3F3A	NP_001014411.1
Bos taurus (cattle)	H3F3B	NP_001229500.1
Pan troglodytes (chimpanzee)	HIST1H3A	NP_001092038.1
Rattus norvegicus (Norway rat)	LOC100361558	XP_002728089.1
Danio rerio (zebrafish)	LOC565028	XP_693431.3

Gene Ontology
Bibliography

Related articles in PubMed

Diagnostic value of histone 3 mutations in osteoclast-rich bone tumors.
Nohr E, Lee LH, Cates JM, Perizzolo M, Itani D
Human pathology68119-127(2017 10)

Probe the function of histone lysine 36 methylation using histone H3 lysine 36 to methionine mutant transgene in mammalian cells.
Fang D, Gan H, Wang H, Zhou H, Zhang Z
Cell cycle (Georgetown, Tex.)16(19)1781-1789(2017 Oct)

Mutation Analysis of H3F3A and H3F3B as a Diagnostic Tool for Giant Cell Tumor of Bone and Chondroblastoma.
Cleven AH, H?cker S, Briaire-de Bruijn I, Szuhai K, Cleton-Jansen AM, Bov?e JV
The American journal of surgical pathology39(11)1576-83(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Report H3F3A/B mutations in cell tumors of bone, chondroblastomas, and aneurysmal bone cysts.
Title: Diagnostic value of histone 3 mutations in osteoclast-rich bone tumors.

H3F3A and H3F3B mutation analysis appears to be a highly specific, although less sensitive, diagnostic tool for the distinction of GCTB and chondroblastoma from other giant cell-containing tumors.
Title: Mutation Analysis of H3F3A and H3F3B as a Diagnostic Tool for Giant Cell Tumor of Bone and Chondroblastoma.

A remarkable picture of tumor type specificity for histone H3.3 driver alterations emerges, indicating that histone H3.3 residues, mutations and genes have distinct functions.
Title: Distinct H3F3A and H3F3B driver mutations define chondroblastoma and giant cell tumor of bone.

DAXX functions as a bona fide histone chaperone involved in the replication-independent deposition of H3.3
Title: The death-associated protein DAXX is a novel histone chaperone involved in the replication-independent deposition of H3.3.

The following H3-3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the H3-3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu24881	NM_005324.5 Latest version!	Homo sapiens H3.3 histone B (H3-3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu24881	NM_005324.4	Homo sapiens H3 histone family member 3B (H3F3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

H3-3B ( NM_005324.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005315.1 Homo sapiens H3 histone family member 3B (H3F3B), mRNA.

Price & Availability↑

CloneID	OHu24881
Clone ID Related Accession (Same CDS sequence)	NM_005324.4 , NM_005324.5
Accession Version	NM_005324.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	histone H3.3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB466880.1, BM723811.1, BX537379.1 and AC087289.9. On Jan 7, 2015 this sequence version replaced NM_005324.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537379.1, SRR1660807.133168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## replication-independent histone :: PMID: 8586426 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCGAA CCAAGCAGAC TGCTCGTAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACGA AAGCCGCCAG GAAAAGCGCT CCCTCTACCG GCGGGGTGAA GAAGCCTCAT 
CGCTACAGGC CCGGGACCGT GGCGCTTCGA GAGATTCGTC GTTATCAGAA GTCGACCGAG 
CTGCTCATCC GGAAGCTGCC CTTCCAGAGG TTGGTGAGGG AGATCGCGCA GGATTTCAAA 
ACCGACCTGA GGTTTCAGAG CGCAGCCATC GGTGCGCTGC AGGAGGCTAG CGAAGCGTAC 
CTGGTGGGTC TGTTCGAAGA TACCAACCTG TGTGCCATCC ACGCTAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGAG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005315.1
CDS	290..700
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_005324.4
Organism	Homo sapiens(human)
Definition	Homo sapiens H3 histone family member 3B (H3F3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005324.4

ORF Insert Sequence:

ATGGCCCGAA CCAAGCAGAC TGCTCGTAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACGA AAGCCGCCAG GAAAAGCGCT CCCTCTACCG GCGGGGTGAA GAAGCCTCAT 
CGCTACAGGC CCGGGACCGT GGCGCTTCGA GAGATTCGTC GTTATCAGAA GTCGACCGAG 
CTGCTCATCC GGAAGCTGCC CTTCCAGAGG TTGGTGAGGG AGATCGCGCA GGATTTCAAA 
ACCGACCTGA GGTTTCAGAG CGCAGCCATC GGTGCGCTGC AGGAGGCTAG CGAAGCGTAC 
CTGGTGGGTC TGTTCGAAGA TACCAACCTG TGTGCCATCC ACGCTAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGAG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

H3-3B ( NM_005324.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005315.1 Homo sapiens H3.3 histone B (H3-3B), mRNA.

Price & Availability↑

CloneID	OHu24881
Clone ID Related Accession (Same CDS sequence)	NM_005324.4 , NM_005324.5
Accession Version	NM_005324.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-02
Organism	Homo sapiens(human)
Product	histone H3.3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087289.9. On Nov 23, 2018 this sequence version replaced NM_005324.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX537379.1, AK130772.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000254810.8/ ENSP00000254810.3 RefSeq Select criteria :: based on single protein-coding transcript replication-independent histone :: PMID: 8586426 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCGAA CCAAGCAGAC TGCTCGTAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACGA AAGCCGCCAG GAAAAGCGCT CCCTCTACCG GCGGGGTGAA GAAGCCTCAT 
CGCTACAGGC CCGGGACCGT GGCGCTTCGA GAGATTCGTC GTTATCAGAA GTCGACCGAG 
CTGCTCATCC GGAAGCTGCC CTTCCAGAGG TTGGTGAGGG AGATCGCGCA GGATTTCAAA 
ACCGACCTGA GGTTTCAGAG CGCAGCCATC GGTGCGCTGC AGGAGGCTAG CGAAGCGTAC 
CTGGTGGGTC TGTTCGAAGA TACCAACCTG TGTGCCATCC ACGCTAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGAG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005315.1
CDS	134..544
Translation	MARTKQTARKSTGGKAPRKQLATKAARKSAPSTGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSAAIGALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Target ORF information:

RefSeq Version	NM_005324.5
Organism	Homo sapiens(human)
Definition	Homo sapiens H3.3 histone B (H3-3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005324.5

ORF Insert Sequence:

ATGGCCCGAA CCAAGCAGAC TGCTCGTAAG TCCACCGGTG GGAAAGCCCC CCGCAAACAG 
CTGGCCACGA AAGCCGCCAG GAAAAGCGCT CCCTCTACCG GCGGGGTGAA GAAGCCTCAT 
CGCTACAGGC CCGGGACCGT GGCGCTTCGA GAGATTCGTC GTTATCAGAA GTCGACCGAG 
CTGCTCATCC GGAAGCTGCC CTTCCAGAGG TTGGTGAGGG AGATCGCGCA GGATTTCAAA 
ACCGACCTGA GGTTTCAGAG CGCAGCCATC GGTGCGCTGC AGGAGGCTAG CGAAGCGTAC 
CTGGTGGGTC TGTTCGAAGA TACCAACCTG TGTGCCATCC ACGCTAAGAG AGTCACCATC 
ATGCCCAAAG ACATCCAGTT GGCTCGCCGG ATACGGGGAG AGAGAGCTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diagnostic value of histone 3 mutations in osteoclast-rich bone tumors.
Human pathology68119-127(2017 10)
Nohr E,Lee LH,Cates JM,Perizzolo M,Itani D

Probe the function of histone lysine 36 methylation using histone H3 lysine 36 to methionine mutant transgene in mammalian cells.
Cell cycle (Georgetown, Tex.)16(19)1781-1789(2017 Oct)
Fang D,Gan H,Wang H,Zhou H,Zhang Z

Mutation Analysis of H3F3A and H3F3B as a Diagnostic Tool for Giant Cell Tumor of Bone and Chondroblastoma.
The American journal of surgical pathology39(11)1576-83(2015 Nov)
Cleven AH,H?cker S,Briaire-de Bruijn I,Szuhai K,Cleton-Jansen AM,Bov?e JV

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H3-3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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