RNF227 cDNA ORF clone, Homo sapiens(human)

The following RNF227 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF227 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu102954 NM_001358699.1
Latest version!
Homo sapiens ring finger protein 227 (RNF227), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu102954
    Clone ID Related Accession (Same CDS sequence) NM_001358699.1
    Accession Version NM_001358699.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Homo sapiens(human)
    Product RING finger protein 227
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC104581.22. On Dec 5, 2017 this sequence version replaced NR_024349.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.131070.1, SRR1660803.82277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAGCTCT TGGTGAGGGT ACCCTCTCTT CCGGAGCGGG GCGAGCTGGA CTGCAACATC 
    TGCTACCGTC CTTTCAACCT CGGGTGCCGC GCGCCCCGCC GCCTGCCCGG AACGGCGCGC
    GCCCGCTGCG GCCACACGAT CTGCACCGCC TGCCTCCGCG AGCTGGCGGC GCGCGGGGAC
    GGCGGCGGGG CGGCCGCGCG CGTGGTGCGC CTGCGCCGCG TGGTCACGTG CCCCTTCTGC
    CGCGCGCCCT CGCAGCTCCC TCGCGGCGGC CTCACGGAGA TGGCTCTTGA CTCGGACTTG
    TGGTCGCGAT TGGAGGAAAA AGCGCGGGCC AAGTGCGAAC GAGATGAGGC TGGGAACCCG
    GCCAAGGAAA GCAGCGACGC TGACGGAGAG GCGGAGGAAG AAGGGGAGAG CGAGAAGGGG
    GCGGGGCCTA GGAGTGCTGG GTGGCGCGCG CTCCGGCGGC TCTGGGACAG GGTCCTGGGG
    CCTGCGCGGC GCTGGCGGCG TCCGCTGCCT AGCAACGTGC TCTACTGTGC GGAGATCAAG
    GACATTGGCC ACCTGACCCG TTGCACGTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001345628.1
    CDS1..573
    Translation

    Target ORF information:

    RefSeq Version NM_001358699.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ring finger protein 227 (RNF227), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001358699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAGCTCT TGGTGAGGGT ACCCTCTCTT CCGGAGCGGG GCGAGCTGGA CTGCAACATC 
    TGCTACCGTC CTTTCAACCT CGGGTGCCGC GCGCCCCGCC GCCTGCCCGG AACGGCGCGC
    GCCCGCTGCG GCCACACGAT CTGCACCGCC TGCCTCCGCG AGCTGGCGGC GCGCGGGGAC
    GGCGGCGGGG CGGCCGCGCG CGTGGTGCGC CTGCGCCGCG TGGTCACGTG CCCCTTCTGC
    CGCGCGCCCT CGCAGCTCCC TCGCGGCGGC CTCACGGAGA TGGCTCTTGA CTCGGACTTG
    TGGTCGCGAT TGGAGGAAAA AGCGCGGGCC AAGTGCGAAC GAGATGAGGC TGGGAACCCG
    GCCAAGGAAA GCAGCGACGC TGACGGAGAG GCGGAGGAAG AAGGGGAGAG CGAGAAGGGG
    GCGGGGCCTA GGAGTGCTGG GTGGCGCGCG CTCCGGCGGC TCTGGGACAG GGTCCTGGGG
    CCTGCGCGGC GCTGGCGGCG TCCGCTGCCT AGCAACGTGC TCTACTGTGC GGAGATCAAG
    GACATTGGCC ACCTGACCCG TTGCACGTTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.
    Nature440(7087)1045-9(2006 Apr)
    Zody MC,Garber M,Adams DJ,Sharpe T,Harrow J,Lupski JR,Nicholson C,Searle SM,Wilming L,Young SK,Abouelleil A,Allen NR,Bi W,Bloom T,Borowsky ML,Bugalter BE,Butler J,Chang JL,Chen CK,Cook A,Corum B,Cuomo CA,de Jong PJ,DeCaprio D,Dewar K,FitzGerald M,Gilbert J,Gibson R,Gnerre S,Goldstein S,Grafham DV,Grocock R,Hafez N,Hagopian DS,Hart E,Norman CH,Humphray S,Jaffe DB,Jones M,Kamal M,Khodiyar VK,LaButti K,Laird G,Lehoczky J,Liu X,Lokyitsang T,Loveland J,Lui A,Macdonald P,Major JE,Matthews L,Mauceli E,McCarroll SA,Mihalev AH,Mudge J,Nguyen C,Nicol R,O'Leary SB,Osoegawa K,Schwartz DC,Shaw-Smith C,Stankiewicz P,Steward C,Swarbreck D,Venkataraman V,Whittaker CA,Yang X,Zimmer AR,Bradley A,Hubbard T,Birren BW,Rogers J,Lander ES,Nusbaum C