GCH1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCH1
Entrez Gene ID	2643
Full Name	GTP cyclohydrolase 1
Synonyms	DYT14,DYT5,DYT5a,GCH,GTP-CH-1,GTPCH1,HPABH4B
General protein information	Preferred Names GTP cyclohydrolase 1 Names GTP cyclohydrolase 1 GTP cyclohydrolase I GTP-CH-I dystonia 14 guanosine 5'-triphosphate cyclohydrolase I
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q22.2
Summary	This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008].
Disorder MIM:	600225
Disorder Html:	Dystonia, DOPA-responsive, with or without hyperphenylalaninemia, 128230 (3); Hyperphenylalaninemia, BH4-deficient, B, 233910 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000161.2	NP_000152.1	GTP cyclohydrolase 1 isoform 1
NM_001024071.1	NP_001019242.1	GTP cyclohydrolase 1 isoform 3
NM_001024070.2	NP_001019241.1	GTP cyclohydrolase 1 isoform 2
NM_001024070.1	NP_001019241.1	GTP cyclohydrolase 1 isoform 2
NM_000161.3	NP_000152.1	GTP cyclohydrolase 1 isoform 1
XM_017021218.1	XP_016876707.1	GTP cyclohydrolase 1 isoform X1
NM_001024071.2	NP_001019242.1	GTP cyclohydrolase 1 isoform 3
NM_001024024.1	NP_001019195.1	GTP cyclohydrolase 1 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GCH1	XP_001161622.1
Mus musculus (house mouse)	Gch1	NP_032128.1
Danio rerio (zebrafish)	gch1	NP_001129727.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006441	XP_316477.4
Saccharomyces cerevisiae (baker's yeast)	FOL2	NP_011783.1
Kluyveromyces lactis	KLLA0A09735g	XP_451425.1
Homo sapiens (human)	GCH1	NP_000152.1
Gallus gallus (chicken)	GCH1	NP_990554.1
Schizosaccharomyces pombe (fission yeast)	SPAC17A5.13	NP_593481.1
Magnaporthe oryzae (rice blast fungus)	MGG_09458	XP_003712296.1
Rattus norvegicus (Norway rat)	Gch1	NP_077332.1
Drosophila melanogaster (fruit fly)	Pu	NP_726038.1
Canis lupus familiaris (dog)	GCH1	XP_005623474.1
Bos taurus (cattle)	GCH1	XP_002690995.1
Xenopus tropicalis (tropical clawed frog)	gch1	NP_001006789.1
	GCH1	XP_001087365.1
Caenorhabditis elegans (roundworm)	cat-4	NP_505710.1
Eremothecium gossypii	AGOS_AGR335C	NP_987001.1
Neurospora crassa	NCU07774	XP_958695.2

Gene Ontology

The following GCH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22538	NM_000161.3 Latest version!	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22538	NM_001024024.1 Latest version!	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu84867	XM_017021218.1 Latest version!	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OHu22905	NM_001024071.2 Latest version!	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu23190	NM_001024070.2 Latest version!	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu23190	NM_001024070.1	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu22538	NM_000161.2	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22905	NM_001024071.1	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCH1 ( NM_000161.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000152.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22538
Clone ID Related Accession (Same CDS sequence)	NM_000161.2 , NM_000161.3 , NM_001024024.1
Accession Version	NM_000161.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5, U19523.1, U66097.1 and BM971258.1. This sequence is a reference standard in the RefSeqGene project. On Jun 3, 2005 this sequence version replaced NM_000161.1. Transcript Variant: This variant (1), also known as Type I, represents the longest transcript. Variants 1 and 2 encode the longest isoform (1), which is the active enzyme. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.64516.1, U66097.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000152.1
CDS	162..914
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Target ORF information:

RefSeq Version	NM_000161.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000161.2

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_000161.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000152.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22538
Clone ID Related Accession (Same CDS sequence)	NM_000161.2 , NM_000161.3 , NM_001024024.1
Accession Version	NM_000161.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5 and AL133444.4. On Nov 22, 2018 this sequence version replaced NM_000161.2. Transcript Variant: This variant (1), also known as Type I, represents the longest transcript. Variants 1 and 2 encode the longest isoform (1), which is the active enzyme. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC025415.2, U66097.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000491895.7/ ENSP00000419045.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000152.1
CDS	164..916
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Target ORF information:

RefSeq Version	NM_000161.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000161.3

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_001024024.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019195.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22538
Clone ID Related Accession (Same CDS sequence)	NM_000161.2 , NM_000161.3 , NM_001024024.1
Accession Version	NM_001024024.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5, AY137463.1, U66095.1 and BM971258.1. Transcript Variant: This variant (2), also known as Type V, differs in the 3' UTR, compared to variant 1. Variants 1 and 2 both encode the functional enzyme, isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD639815.1, AY137463.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019195.1
CDS	162..914
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Target ORF information:

RefSeq Version	NM_001024024.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024024.1

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACACAC ATGTGTATGG TAATGCGAGG TGTACAGAAA 
ATGAACAGCA AAACTGTGAC CAGCACAATG TTGGGTGTGT TCCGGGAGGA TCCAAAGACT 
CGGGAAGAGT TCCTGACTCT CATTAGGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( XM_017021218.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876707.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu84867
Clone ID Related Accession (Same CDS sequence)	XM_017021218.1
Accession Version	XM_017021218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGGGT TCATGCTTAT TTTGGCCAAG TTTTCCAAAT TCTGTCTTGA TGTCCTAAAC 
GATGCTATAT TTGATGAAGA TCATGATGAG ATGGTGATTG TGAAGGACAT AGACATGTTT 
TCCATGTGTG AGCATCACTT GGTTCCATTT GTTGGAAAGG TCCATATTGG TTATCTTCCT 
AACAAGCAAG TCCTTGGCCT CAGCAAACTT GCGAGGATTG TAGAAATCTA TAGTAGAAGA 
CTACAAGTTC AGGAGCGCCT TACAAAACAA ATTGCTGTAG CAATCACGGA AGCCTTGCGG 
CCTGCTGGAG TCGGGGTAGT GGTTGAAGCA ACACACATGT GTATGGTAAT GCGAGGTGTA 
CAGAAAATGA ACAGCAAAAC TGTGACCAGC ACAATGTTGG GTGTGTTCCG GGAGGATCCA 
AAGACTCGGG AAGAGTTCCT GACTCTCATT AGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876707.1
CDS	888..1346
Translation	MFGFMLILAKFSKFCLDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATHMCMVMRGVQKMNSKTVTSTMLGVFREDPKTREEFLTLIRS

Target ORF information:

RefSeq Version	XM_017021218.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021218.1

ORF Insert Sequence:

ATGTTTGGGT TCATGCTTAT TTTGGCCAAG TTTTCCAAAT TCTGTCTTGA TGTCCTAAAC 
GATGCTATAT TTGATGAAGA TCATGATGAG ATGGTGATTG TGAAGGACAT AGACATGTTT 
TCCATGTGTG AGCATCACTT GGTTCCATTT GTTGGAAAGG TCCATATTGG TTATCTTCCT 
AACAAGCAAG TCCTTGGCCT CAGCAAACTT GCGAGGATTG TAGAAATCTA TAGTAGAAGA 
CTACAAGTTC AGGAGCGCCT TACAAAACAA ATTGCTGTAG CAATCACGGA AGCCTTGCGG 
CCTGCTGGAG TCGGGGTAGT GGTTGAAGCA ACACACATGT GTATGGTAAT GCGAGGTGTA 
CAGAAAATGA ACAGCAAAAC TGTGACCAGC ACAATGTTGG GTGTGTTCCG GGAGGATCCA 
AAGACTCGGG AAGAGTTCCT GACTCTCATT AGGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_001024071.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019242.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22905
Clone ID Related Accession (Same CDS sequence)	NM_001024071.1 , NM_001024071.2
Accession Version	NM_001024071.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5, BC025415.2, Z29433.2 and BM971258.1. Transcript Variant: This variant (4), also known as Type II, lacks an alternate in-frame exon in the 3' coding region and uses a downstream stop codon, compared to variant 1. The resulting non-functional protein (isoform 3) is shorter and has a distinct C-terminus, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z29433.2, SRR1163657.387648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGTCT GCAGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019242.1
CDS	162..803
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATSAEP

Target ORF information:

RefSeq Version	NM_001024071.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024071.1

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGTCT GCAGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_001024071.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019242.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22905
Clone ID Related Accession (Same CDS sequence)	NM_001024071.1 , NM_001024071.2
Accession Version	NM_001024071.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5 and AL133444.4. On Jun 1, 2019 this sequence version replaced NM_001024071.1. Transcript Variant: This variant (4), also known as Type II, lacks an alternate in-frame exon in the 3' coding region and uses a downstream stop codon, compared to variant 1. The resulting non-functional protein (isoform 3) is shorter and has a distinct C-terminus, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Z29433.2, SRR1163657.387648.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGTCT GCAGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019242.1
CDS	164..805
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATSAEP

Target ORF information:

RefSeq Version	NM_001024071.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024071.2

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGTCT GCAGAACCAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_001024070.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019241.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23190
Clone ID Related Accession (Same CDS sequence)	NM_001024070.2 , NM_001024070.1
Accession Version	NM_001024070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5 and AL133444.4. On May 31, 2019 this sequence version replaced NM_001024070.1. Transcript Variant: This variant (3), also known as Type IV, uses alternate, in-frame splice sites in the 3' coding region and 3' UTR, compared to variant 1. The resulting non-functional protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66095.1, SRR1163658.73793.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGAAG TCAAATAAAT ATAATAAAGG GTTGAGCCCT 
CTACTTTCTT CTTGCCACCT TTTTGTGGCA ATATTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019241.1
CDS	164..865
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATKSNKYNKGLSPLLSSCHLFVAILK

Target ORF information:

RefSeq Version	NM_001024070.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024070.2

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGAAG TCAAATAAAT ATAATAAAGG GTTGAGCCCT 
CTACTTTCTT CTTGCCACCT TTTTGTGGCA ATATTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GCH1 ( NM_001024070.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001019241.1 Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23190
Clone ID Related Accession (Same CDS sequence)	NM_001024070.2 , NM_001024070.1
Accession Version	NM_001024070.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 702bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	GTP cyclohydrolase 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL160471.5, U66095.1 and BM971258.1. Transcript Variant: This variant (3), also known as Type IV, uses alternate, in-frame splice sites in the 3' coding region and 3' UTR, compared to variant 1. The resulting non-functional protein (isoform 2) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U66095.1, SRR1163658.73793.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGAAG TCAAATAAAT ATAATAAAGG GTTGAGCCCT 
CTACTTTCTT CTTGCCACCT TTTTGTGGCA ATATTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019241.1
CDS	162..863
Translation	MEKGPVRAPAEKPRGARCSNGFPERDPPRPGPSRPAEKPPRPEAKSAQPADGWKGERPRSEEDNELNLPNLAAAYSSILSSLGENPQRQGLLKTPWRAASAMQFFTKGYQETISDVLNDAIFDEDHDEMVIVKDIDMFSMCEHHLVPFVGKVHIGYLPNKQVLGLSKLARIVEIYSRRLQVQERLTKQIAVAITEALRPAGVGVVVEATKSNKYNKGLSPLLSSCHLFVAILK

Target ORF information:

RefSeq Version	NM_001024070.1
Organism	Homo sapiens(human)
Definition	Homo sapiens GTP cyclohydrolase 1 (GCH1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024070.1

ORF Insert Sequence:

ATGGAGAAGG GCCCTGTGCG GGCACCGGCG GAGAAGCCGC GGGGCGCCAG GTGCAGCAAT 
GGGTTCCCCG AGCGGGATCC GCCGCGGCCC GGGCCCAGCA GGCCGGCGGA GAAGCCCCCG 
CGGCCCGAGG CCAAGAGCGC GCAGCCCGCG GACGGCTGGA AGGGCGAGCG GCCCCGCAGC 
GAGGAGGATA ACGAGCTGAA CCTCCCTAAC CTGGCAGCCG CCTACTCGTC CATCCTGAGC 
TCGCTGGGCG AGAACCCCCA GCGGCAAGGG CTGCTCAAGA CGCCCTGGAG GGCGGCCTCG 
GCCATGCAGT TCTTCACCAA GGGCTACCAG GAGACCATCT CAGATGTCCT AAACGATGCT 
ATATTTGATG AAGATCATGA TGAGATGGTG ATTGTGAAGG ACATAGACAT GTTTTCCATG 
TGTGAGCATC ACTTGGTTCC ATTTGTTGGA AAGGTCCATA TTGGTTATCT TCCTAACAAG 
CAAGTCCTTG GCCTCAGCAA ACTTGCGAGG ATTGTAGAAA TCTATAGTAG AAGACTACAA 
GTTCAGGAGC GCCTTACAAA ACAAATTGCT GTAGCAATCA CGGAAGCCTT GCGGCCTGCT 
GGAGTCGGGG TAGTGGTTGA AGCAACGAAG TCAAATAAAT ATAATAAAGG GTTGAGCCCT 
CTACTTTCTT CTTGCCACCT TTTTGTGGCA ATATTAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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GCH1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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