BHMT2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BHMT2
Entrez Gene ID	23743
Full Name	betaine--homocysteine S-methyltransferase 2
General protein information	Preferred Names betaine--homocysteine S-methyltransferase 2 Names S-methylmethionine--homocysteine S-methyltransferase BHMT2 SMM-hcy methyltransferase betaine-homocysteine methyltransferase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q14.1
Summary	Homocysteine is a sulfur-containing amino acid that plays a crucial role in methylation reactions. Transfer of the methyl group from betaine to homocysteine creates methionine, which donates the methyl group to methylate DNA, proteins, lipids, and other intracellular metabolites. The protein encoded by this gene is one of two methyl transferases that can catalyze the transfer of the methyl group from betaine to homocysteine. Anomalies in homocysteine metabolism have been implicated in disorders ranging from vascular disease to neural tube birth defects such as spina bifida. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010].
Disorder MIM:	605932

mRNA and Protein(s)

mRNA	Protein	Name
NM_001178005.1	NP_001171476.1	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 2
NM_017614.4	NP_060084.2	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 1
NM_017614.5	NP_060084.2	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 1
NM_001178005.2	NP_001171476.1	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	BHMT2	XP_527207.2
Rattus norvegicus (Norway rat)	Bhmt2	NP_001014278.2
Homo sapiens (human)	BHMT2	NP_060084.2
Bos taurus (cattle)	BHMT2	XP_003586562.2
	BHMT2	XP_001103987.1
Canis lupus familiaris (dog)	BHMT2	XP_546051.2
Mus musculus (house mouse)	Bhmt2	NP_075022.2

Gene Ontology
Bibliography

Related articles in PubMed

Evidence of interaction between genes in the folate/homocysteine metabolic pathway in controlling risk of nonsyndromic oral cleft.
Wang P, Wu T, Schwender H, Wang H, Shi B, Wang Z, Yuan Y, Liu D, Wang M, Li J, Zhou Z, Zhu H, Beaty TH
Oral diseases(2018 Jan)

A Three-Way Interaction among Maternal and Fetal Variants Contributing to Congenital Heart Defects.
Li M, Li J, Wei C, Lu Q, Tang X, Erickson SW, MacLeod SL, Hobbs CA
Annals of human genetics80(1)20-31(2016 Jan)

Obstructive heart defects associated with candidate genes, maternal obesity, and folic acid supplementation.
Tang X, Cleves MA, Nick TG, Li M, MacLeod SL, Erickson SW, Li J, Shaw GM, Mosley BS, Hobbs CA,
American journal of medical genetics. Part A167(6)1231-42(2015 Jun)

Genome-wide association study of selenium concentrations.
Cornelis MC, Fornage M, Foy M, Xun P, Gladyshev VN, Morris S, Chasman DI, Hu FB, Rimm EB, Kraft P, Jordan JM, Mozaffarian D, He K
Human molecular genetics24(5)1469-77(2015 Mar)

Evolutionary Analyses and Natural Selection of Betaine-Homocysteine S-Methyltransferase (BHMT) and BHMT2 Genes.
Ganu RS, Ishida Y, Koutmos M, Kolokotronis SO, Roca AL, Garrow TA, Schook LB
PloS one10(7)e0134084(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A three-way interaction among maternal and fetal variants in BHMT2, GSTP1 and GPX3 contribute to congenital heart defects
Title: A Three-Way Interaction among Maternal and Fetal Variants Contributing to Congenital Heart Defects.

Multiple SNPs in BHMT and BHMT2 were identified to be associated with the occurrence of infant obstructive heart defects and interaction effects with maternal use of folic acid supplements.
Title: Obstructive heart defects associated with candidate genes, maternal obesity, and folic acid supplementation.

The faster evolutionary rate of BHMT2 overall suggests that selective constraints were reduced relative to BHMT.
Title: Evolutionary Analyses and Natural Selection of Betaine-Homocysteine S-Methyltransferase (BHMT) and BHMT2 Genes.

No significant level of association was found with cleft lip with or without cleft palate and BHMT2 variants.
Title: New evidence for the role of cystathionine beta-synthase in non-syndromic cleft lip with or without cleft palate.

Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
Title: Polymorphisms located in the region containing BHMT and BHMT2 genes as maternal protective factors for orofacial clefts.

The following BHMT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BHMT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21593	NM_017614.5 Latest version!	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14381	NM_001178005.2 Latest version!	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu21593	NM_017614.4	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14381	NM_001178005.1	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BHMT2 ( NM_017614.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060084.2 Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21593
Clone ID Related Accession (Same CDS sequence)	NM_017614.4 , NM_017614.5
Accession Version	NM_017614.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB140104.1, BC020665.1 and AC008502.8. This sequence is a reference standard in the RefSeqGene project. On May 6, 2010 this sequence version replaced NM_017614.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.205463.1, SRR1803611.123018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTG GGAAGATGTA AATGCTGCTG CCTGTGACCT CGCCAGGGAA 
GTGGCTGGCA AAGGTGATGC TTTGGTAGCA GGGGGGATCT GCCAGACATC AATATACAAA 
TACCAGAAGG ATGAAGCTAG AATTAAAAAA CTTTTTCGAC AACAGCTAGA AGTTTTTGCC 
TGGAAAAATG TGGACTTCTT GATTGCAGAG TATTTTGAGC ACGTTGAAGA AGCTGTGTGG 
GCTGTGGAAG TCTTAAAAGA ATCAGATAGA CCCGTGGCAG TTACCATGTG CATAGGCCCA 
GAGGGAGACA TGCATGATAT AACCCCCGGA GAATGTGCTG TGAGGCTGGT GAAGGCAGGG 
GCTTCCATCG TTGGCGTGAA CTGCCGCTTT GGGCCCGACA CCAGCTTGAA GACGATGGAG 
CTCATGAAGG AGGGTCTTGA GTGGGCAGGG CTGAAAGCGC ACCTCATGGT GCAGCCTCTG 
GGGTTCCACG CGCCTGACTG TGGCAAAGAG GGGTTTGTGG ATCTCCCAGA ATATCCCTTT 
GGACTGGAGT CCAGAGTTGC CACCAGATGG GATATTCAAA AATACGCCAG AGAGGCCTAC 
AACCTGGGGG TCAGGTACAT TGGCGGGTGC TGTGGATTTG AGCCCTACCA CATCAGGGCA 
ATTGCAGAGG AGCTGGCCCC AGAAAGGGGC TTTTTGCCAC CAGCTTCAGA AAAACACGGC 
AGCTGGGGAA GTGGTTTGGA CATGCACACC AAACCCTGGA TTAGAGCAAG GGCTCGAAGG 
GAGTATTGGG AGAATCTGCT GCCAGCTTCA GGCAGACCTT TCTGTCCTTC GCTGTCAAAG 
CCAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060084.2
CDS	60..1151
Translation	MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFAWKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF

Target ORF information:

RefSeq Version	NM_017614.4
Organism	Homo sapiens(human)
Definition	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017614.4

ORF Insert Sequence:

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTG GGAAGATGTA AATGCTGCTG CCTGTGACCT CGCCAGGGAA 
GTGGCTGGCA AAGGTGATGC TTTGGTAGCA GGGGGGATCT GCCAGACATC AATATACAAA 
TACCAGAAGG ATGAAGCTAG AATTAAAAAA CTTTTTCGAC AACAGCTAGA AGTTTTTGCC 
TGGAAAAATG TGGACTTCTT GATTGCAGAG TATTTTGAGC ACGTTGAAGA AGCTGTGTGG 
GCTGTGGAAG TCTTAAAAGA ATCAGATAGA CCCGTGGCAG TTACCATGTG CATAGGCCCA 
GAGGGAGACA TGCATGATAT AACCCCCGGA GAATGTGCTG TGAGGCTGGT GAAGGCAGGG 
GCTTCCATCG TTGGCGTGAA CTGCCGCTTT GGGCCCGACA CCAGCTTGAA GACGATGGAG 
CTCATGAAGG AGGGTCTTGA GTGGGCAGGG CTGAAAGCGC ACCTCATGGT GCAGCCTCTG 
GGGTTCCACG CGCCTGACTG TGGCAAAGAG GGGTTTGTGG ATCTCCCAGA ATATCCCTTT 
GGACTGGAGT CCAGAGTTGC CACCAGATGG GATATTCAAA AATACGCCAG AGAGGCCTAC 
AACCTGGGGG TCAGGTACAT TGGCGGGTGC TGTGGATTTG AGCCCTACCA CATCAGGGCA 
ATTGCAGAGG AGCTGGCCCC AGAAAGGGGC TTTTTGCCAC CAGCTTCAGA AAAACACGGC 
AGCTGGGGAA GTGGTTTGGA CATGCACACC AAACCCTGGA TTAGAGCAAG GGCTCGAAGG 
GAGTATTGGG AGAATCTGCT GCCAGCTTCA GGCAGACCTT TCTGTCCTTC GCTGTCAAAG 
CCAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BHMT2 ( NM_017614.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060084.2 Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21593
Clone ID Related Accession (Same CDS sequence)	NM_017614.4 , NM_017614.5
Accession Version	NM_017614.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB140104.1, BC020665.1 and AC008502.8. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_017614.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.205463.1, SRR1803612.275269.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000255192.8/ ENSP00000255192.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTG GGAAGATGTA AATGCTGCTG CCTGTGACCT CGCCAGGGAA 
GTGGCTGGCA AAGGTGATGC TTTGGTAGCA GGGGGGATCT GCCAGACATC AATATACAAA 
TACCAGAAGG ATGAAGCTAG AATTAAAAAA CTTTTTCGAC AACAGCTAGA AGTTTTTGCC 
TGGAAAAATG TGGACTTCTT GATTGCAGAG TATTTTGAGC ACGTTGAAGA AGCTGTGTGG 
GCTGTGGAAG TCTTAAAAGA ATCAGATAGA CCCGTGGCAG TTACCATGTG CATAGGCCCA 
GAGGGAGACA TGCATGATAT AACCCCCGGA GAATGTGCTG TGAGGCTGGT GAAGGCAGGG 
GCTTCCATCG TTGGCGTGAA CTGCCGCTTT GGGCCCGACA CCAGCTTGAA GACGATGGAG 
CTCATGAAGG AGGGTCTTGA GTGGGCAGGG CTGAAAGCGC ACCTCATGGT GCAGCCTCTG 
GGGTTCCACG CGCCTGACTG TGGCAAAGAG GGGTTTGTGG ATCTCCCAGA ATATCCCTTT 
GGACTGGAGT CCAGAGTTGC CACCAGATGG GATATTCAAA AATACGCCAG AGAGGCCTAC 
AACCTGGGGG TCAGGTACAT TGGCGGGTGC TGTGGATTTG AGCCCTACCA CATCAGGGCA 
ATTGCAGAGG AGCTGGCCCC AGAAAGGGGC TTTTTGCCAC CAGCTTCAGA AAAACACGGC 
AGCTGGGGAA GTGGTTTGGA CATGCACACC AAACCCTGGA TTAGAGCAAG GGCTCGAAGG 
GAGTATTGGG AGAATCTGCT GCCAGCTTCA GGCAGACCTT TCTGTCCTTC GCTGTCAAAG 
CCAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060084.2
CDS	17..1108
Translation	MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKWEDVNAAACDLAREVAGKGDALVAGGICQTSIYKYQKDEARIKKLFRQQLEVFAWKNVDFLIAEYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF

Target ORF information:

RefSeq Version	NM_017614.5
Organism	Homo sapiens(human)
Definition	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017614.5

ORF Insert Sequence:

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTG GGAAGATGTA AATGCTGCTG CCTGTGACCT CGCCAGGGAA 
GTGGCTGGCA AAGGTGATGC TTTGGTAGCA GGGGGGATCT GCCAGACATC AATATACAAA 
TACCAGAAGG ATGAAGCTAG AATTAAAAAA CTTTTTCGAC AACAGCTAGA AGTTTTTGCC 
TGGAAAAATG TGGACTTCTT GATTGCAGAG TATTTTGAGC ACGTTGAAGA AGCTGTGTGG 
GCTGTGGAAG TCTTAAAAGA ATCAGATAGA CCCGTGGCAG TTACCATGTG CATAGGCCCA 
GAGGGAGACA TGCATGATAT AACCCCCGGA GAATGTGCTG TGAGGCTGGT GAAGGCAGGG 
GCTTCCATCG TTGGCGTGAA CTGCCGCTTT GGGCCCGACA CCAGCTTGAA GACGATGGAG 
CTCATGAAGG AGGGTCTTGA GTGGGCAGGG CTGAAAGCGC ACCTCATGGT GCAGCCTCTG 
GGGTTCCACG CGCCTGACTG TGGCAAAGAG GGGTTTGTGG ATCTCCCAGA ATATCCCTTT 
GGACTGGAGT CCAGAGTTGC CACCAGATGG GATATTCAAA AATACGCCAG AGAGGCCTAC 
AACCTGGGGG TCAGGTACAT TGGCGGGTGC TGTGGATTTG AGCCCTACCA CATCAGGGCA 
ATTGCAGAGG AGCTGGCCCC AGAAAGGGGC TTTTTGCCAC CAGCTTCAGA AAAACACGGC 
AGCTGGGGAA GTGGTTTGGA CATGCACACC AAACCCTGGA TTAGAGCAAG GGCTCGAAGG 
GAGTATTGGG AGAATCTGCT GCCAGCTTCA GGCAGACCTT TCTGTCCTTC GCTGTCAAAG 
CCAGACTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BHMT2 ( NM_001178005.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171476.1 Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14381
Clone ID Related Accession (Same CDS sequence)	NM_001178005.1 , NM_001178005.2
Accession Version	NM_001178005.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-09
Organism	Homo sapiens(human)
Product	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB140104.1, BC020665.1, AK298298.1 and AC008502.8. Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTA TTTTGAGCAC GTTGAAGAAG CTGTGTGGGC TGTGGAAGTC 
TTAAAAGAAT CAGATAGACC CGTGGCAGTT ACCATGTGCA TAGGCCCAGA GGGAGACATG 
CATGATATAA CCCCCGGAGA ATGTGCTGTG AGGCTGGTGA AGGCAGGGGC TTCCATCGTT 
GGCGTGAACT GCCGCTTTGG GCCCGACACC AGCTTGAAGA CGATGGAGCT CATGAAGGAG 
GGTCTTGAGT GGGCAGGGCT GAAAGCGCAC CTCATGGTGC AGCCTCTGGG GTTCCACGCG 
CCTGACTGTG GCAAAGAGGG GTTTGTGGAT CTCCCAGAAT ATCCCTTTGG ACTGGAGTCC 
AGAGTTGCCA CCAGATGGGA TATTCAAAAA TACGCCAGAG AGGCCTACAA CCTGGGGGTC 
AGGTACATTG GCGGGTGCTG TGGATTTGAG CCCTACCACA TCAGGGCAAT TGCAGAGGAG 
CTGGCCCCAG AAAGGGGCTT TTTGCCACCA GCTTCAGAAA AACACGGCAG CTGGGGAAGT 
GGTTTGGACA TGCACACCAA ACCCTGGATT AGAGCAAGGG CTCGAAGGGA GTATTGGGAG 
AATCTGCTGC CAGCTTCAGG CAGACCTTTC TGTCCTTCGC TGTCAAAGCC AGACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171476.1
CDS	60..959
Translation	MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF

Target ORF information:

RefSeq Version	NM_001178005.1
Organism	Homo sapiens(human)
Definition	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178005.1

ORF Insert Sequence:

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTA TTTTGAGCAC GTTGAAGAAG CTGTGTGGGC TGTGGAAGTC 
TTAAAAGAAT CAGATAGACC CGTGGCAGTT ACCATGTGCA TAGGCCCAGA GGGAGACATG 
CATGATATAA CCCCCGGAGA ATGTGCTGTG AGGCTGGTGA AGGCAGGGGC TTCCATCGTT 
GGCGTGAACT GCCGCTTTGG GCCCGACACC AGCTTGAAGA CGATGGAGCT CATGAAGGAG 
GGTCTTGAGT GGGCAGGGCT GAAAGCGCAC CTCATGGTGC AGCCTCTGGG GTTCCACGCG 
CCTGACTGTG GCAAAGAGGG GTTTGTGGAT CTCCCAGAAT ATCCCTTTGG ACTGGAGTCC 
AGAGTTGCCA CCAGATGGGA TATTCAAAAA TACGCCAGAG AGGCCTACAA CCTGGGGGTC 
AGGTACATTG GCGGGTGCTG TGGATTTGAG CCCTACCACA TCAGGGCAAT TGCAGAGGAG 
CTGGCCCCAG AAAGGGGCTT TTTGCCACCA GCTTCAGAAA AACACGGCAG CTGGGGAAGT 
GGTTTGGACA TGCACACCAA ACCCTGGATT AGAGCAAGGG CTCGAAGGGA GTATTGGGAG 
AATCTGCTGC CAGCTTCAGG CAGACCTTTC TGTCCTTCGC TGTCAAAGCC AGACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BHMT2 ( NM_001178005.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171476.1 Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.

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CloneID	OHu14381
Clone ID Related Accession (Same CDS sequence)	NM_001178005.1 , NM_001178005.2
Accession Version	NM_001178005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	S-methylmethionine--homocysteine S-methyltransferase BHMT2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB140104.1, BC020665.1, AK298298.1 and AC008502.8. On May 31, 2019 this sequence version replaced NM_001178005.1. Transcript Variant: This variant (2) lacks an in-frame exon in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK298298.1, SRR5189667.364201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END##

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTA TTTTGAGCAC GTTGAAGAAG CTGTGTGGGC TGTGGAAGTC 
TTAAAAGAAT CAGATAGACC CGTGGCAGTT ACCATGTGCA TAGGCCCAGA GGGAGACATG 
CATGATATAA CCCCCGGAGA ATGTGCTGTG AGGCTGGTGA AGGCAGGGGC TTCCATCGTT 
GGCGTGAACT GCCGCTTTGG GCCCGACACC AGCTTGAAGA CGATGGAGCT CATGAAGGAG 
GGTCTTGAGT GGGCAGGGCT GAAAGCGCAC CTCATGGTGC AGCCTCTGGG GTTCCACGCG 
CCTGACTGTG GCAAAGAGGG GTTTGTGGAT CTCCCAGAAT ATCCCTTTGG ACTGGAGTCC 
AGAGTTGCCA CCAGATGGGA TATTCAAAAA TACGCCAGAG AGGCCTACAA CCTGGGGGTC 
AGGTACATTG GCGGGTGCTG TGGATTTGAG CCCTACCACA TCAGGGCAAT TGCAGAGGAG 
CTGGCCCCAG AAAGGGGCTT TTTGCCACCA GCTTCAGAAA AACACGGCAG CTGGGGAAGT 
GGTTTGGACA TGCACACCAA ACCCTGGATT AGAGCAAGGG CTCGAAGGGA GTATTGGGAG 
AATCTGCTGC CAGCTTCAGG CAGACCTTTC TGTCCTTCGC TGTCAAAGCC AGACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171476.1
CDS	17..916
Translation	MAPAGRPGAKKGILERLESGEVVIGDGSFLITLEKRGYVKAGLWTPEAVIEHPDAVRQLHMEFLRAGSNVMQTFTFSASEDNMESKYFEHVEEAVWAVEVLKESDRPVAVTMCIGPEGDMHDITPGECAVRLVKAGASIVGVNCRFGPDTSLKTMELMKEGLEWAGLKAHLMVQPLGFHAPDCGKEGFVDLPEYPFGLESRVATRWDIQKYAREAYNLGVRYIGGCCGFEPYHIRAIAEELAPERGFLPPASEKHGSWGSGLDMHTKPWIRARARREYWENLLPASGRPFCPSLSKPDF

Target ORF information:

RefSeq Version	NM_001178005.2
Organism	Homo sapiens(human)
Definition	Homo sapiens betaine--homocysteine S-methyltransferase 2 (BHMT2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178005.2

ORF Insert Sequence:

ATGGCACCTG CTGGACGCCC GGGGGCCAAG AAGGGGATTT TGGAGCGCCT GGAGAGTGGG 
GAGGTTGTGA TTGGAGATGG CAGCTTTCTC ATTACTCTGG AGAAGAGAGG CTATGTGAAG 
GCTGGGCTCT GGACTCCAGA GGCAGTGATA GAACACCCAG ACGCAGTTCG TCAACTTCAC 
ATGGAATTCT TGAGAGCAGG ATCAAATGTC ATGCAGACTT TTACCTTTTC TGCCAGTGAG 
GACAATATGG AAAGCAAGTA TTTTGAGCAC GTTGAAGAAG CTGTGTGGGC TGTGGAAGTC 
TTAAAAGAAT CAGATAGACC CGTGGCAGTT ACCATGTGCA TAGGCCCAGA GGGAGACATG 
CATGATATAA CCCCCGGAGA ATGTGCTGTG AGGCTGGTGA AGGCAGGGGC TTCCATCGTT 
GGCGTGAACT GCCGCTTTGG GCCCGACACC AGCTTGAAGA CGATGGAGCT CATGAAGGAG 
GGTCTTGAGT GGGCAGGGCT GAAAGCGCAC CTCATGGTGC AGCCTCTGGG GTTCCACGCG 
CCTGACTGTG GCAAAGAGGG GTTTGTGGAT CTCCCAGAAT ATCCCTTTGG ACTGGAGTCC 
AGAGTTGCCA CCAGATGGGA TATTCAAAAA TACGCCAGAG AGGCCTACAA CCTGGGGGTC 
AGGTACATTG GCGGGTGCTG TGGATTTGAG CCCTACCACA TCAGGGCAAT TGCAGAGGAG 
CTGGCCCCAG AAAGGGGCTT TTTGCCACCA GCTTCAGAAA AACACGGCAG CTGGGGAAGT 
GGTTTGGACA TGCACACCAA ACCCTGGATT AGAGCAAGGG CTCGAAGGGA GTATTGGGAG 
AATCTGCTGC CAGCTTCAGG CAGACCTTTC TGTCCTTCGC TGTCAAAGCC AGACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evidence of interaction between genes in the folate/homocysteine metabolic pathway in controlling risk of nonsyndromic oral cleft.
Oral diseases(2018 Jan)
Wang P,Wu T,Schwender H,Wang H,Shi B,Wang Z,Yuan Y,Liu D,Wang M,Li J,Zhou Z,Zhu H,Beaty TH

A Three-Way Interaction among Maternal and Fetal Variants Contributing to Congenital Heart Defects.
Annals of human genetics80(1)20-31(2016 Jan)
Li M,Li J,Wei C,Lu Q,Tang X,Erickson SW,MacLeod SL,Hobbs CA

Obstructive heart defects associated with candidate genes, maternal obesity, and folic acid supplementation.
American journal of medical genetics. Part A167(6)1231-42(2015 Jun)
Tang X,Cleves MA,Nick TG,Li M,MacLeod SL,Erickson SW,Li J,Shaw GM,Mosley BS,Hobbs CA,

Genome-wide association study of selenium concentrations.
Human molecular genetics24(5)1469-77(2015 Mar)
Cornelis MC,Fornage M,Foy M,Xun P,Gladyshev VN,Morris S,Chasman DI,Hu FB,Rimm EB,Kraft P,Jordan JM,Mozaffarian D,He K

Evolutionary Analyses and Natural Selection of Betaine-Homocysteine S-Methyltransferase (BHMT) and BHMT2 Genes.
PloS one10(7)e0134084(2015)
Ganu RS,Ishida Y,Koutmos M,Kolokotronis SO,Roca AL,Garrow TA,Schook LB

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BHMT2 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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