PRAME cDNA ORF clone, Homo sapiens(human)

The following PRAME gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRAME cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17035 NM_001291716.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_206954.3
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_006115.5
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_206953.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_206955.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_206956.3
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_001291715.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17036 NM_001291717.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 NM_001291719.2
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 NM_001318127.1
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 NM_001318126.1
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 XM_011530034.3
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 XM_011546898.1
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 XM_011530035.1
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 XM_011546897.1
Latest version!
Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
View Old Accession Versions >>
OHu17035 NM_006115.4 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17036 XM_011530034.1 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17035 NM_001291715.1 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17036 NM_001291719.1 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17035 NM_206954.2 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17035 NM_001291716.1 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu17036 XM_011530034.2 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17036 NM_001291717.1 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu17035 NM_206956.2 Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_006115.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1. On May 31, 2019 this sequence version replaced NM_006115.4. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U65011.1, SRR5189661.135322.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006106.1
    CDS226..1755
    Translation

    Target ORF information:

    RefSeq Version NM_006115.5
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006115.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_001291715.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1 and BC022008.1. On Jun 1, 2019 this sequence version replaced NM_001291715.1. Transcript Variant: This variant (6) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.183264.1, SRR5189667.148956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278644.1
    CDS187..1716
    Translation

    Target ORF information:

    RefSeq Version NM_001291715.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291715.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_001291716.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1 and BC022008.1. On May 31, 2019 this sequence version replaced NM_001291716.1. Transcript Variant: This variant (7) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278645.1
    CDS166..1695
    Translation

    Target ORF information:

    RefSeq Version NM_001291716.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291716.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_006115.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced NM_006115.3. Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U65011.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_006106.1
    CDS322..1851
    Translation

    Target ORF information:

    RefSeq Version NM_006115.4
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006115.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206956.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-28
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1, DB024053.1, AK304593.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced NM_206956.1. Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014074.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996839.1
    CDS505..2034
    Translation

    Target ORF information:

    RefSeq Version NM_206956.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206956.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206955.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1, DB024053.1, AK304593.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced NM_206955.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.213451.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996838.1
    CDS526..2055
    Translation

    Target ORF information:

    RefSeq Version NM_206955.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206955.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_001291715.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1, BC022008.1 and AI613210.1. Transcript Variant: This variant (6) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK129783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278644.1
    CDS309..1838
    Translation

    Target ORF information:

    RefSeq Version NM_001291715.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291715.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206954.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1. On May 31, 2019 this sequence version replaced NM_206954.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.178601.1, SRR7346977.1107929.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996837.1
    CDS205..1734
    Translation

    Target ORF information:

    RefSeq Version NM_206954.3
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206954.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206954.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced NM_206954.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.70294.1, SRR5189661.141310.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996837.1
    CDS301..1830
    Translation

    Target ORF information:

    RefSeq Version NM_206954.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206954.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_001291716.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1, BC022008.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced XM_006725402.1. Transcript Variant: This variant (7) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278645.1
    CDS288..1817
    Translation

    Target ORF information:

    RefSeq Version NM_001291716.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291716.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206956.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1, DB024053.1 and AK304593.1. On Nov 23, 2018 this sequence version replaced NM_206956.2. Transcript Variant: This variant (5) differs in the 5' UTR, compared to variant 2. Variants 1-7 encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.183151.1, SRR5189661.160769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000405655.8/ ENSP00000384343.3 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996839.1
    CDS409..1938
    Translation

    Target ORF information:

    RefSeq Version NM_206956.3
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206956.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17035
    Clone ID Related Accession (Same CDS sequence) NM_006115.5 , NM_001291715.2 , NM_001291716.2 , NM_006115.4 , NM_206956.2 , NM_206955.2 , NM_001291715.1 , NM_206954.3 , NM_206954.2 , NM_001291716.1 , NM_206956.3 , NM_206953.2
    Accession Version NM_206953.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1 and AI613210.1. On Apr 25, 2014 this sequence version replaced NM_206953.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longer isoform (a). Variants 1-7 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996836.1
    CDS936..2465
    Translation

    Target ORF information:

    RefSeq Version NM_206953.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206953.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAACGAA GGCGTTTGTG GGGTTCCATT CAGAGCCGAT ACATCAGCAT GAGTGTGTGG 
    ACAAGCCCAC GGAGACTTGT GGAGCTGGCA GGGCAGAGCC TGCTGAAGGA TGAGGCCCTG
    GCCATTGCCG CCCTGGAGTT GCTGCCCAGG GAGCTCTTCC CGCCACTCTT CATGGCAGCC
    TTTGACGGGA GACACAGCCA GACCCTGAAG GCAATGGTGC AGGCCTGGCC CTTCACCTGC
    CTCCCTCTGG GAGTGCTGAT GAAGGGACAA CATCTTCACC TGGAGACCTT CAAAGCTGTG
    CTTGATGGAC TTGATGTGCT CCTTGCCCAG GAGGTTCGCC CCAGGAGGTG GAAACTTCAA
    GTGCTGGATT TACGGAAGAA CTCTCATCAG GACTTCTGGA CTGTATGGTC TGGAAACAGG
    GCCAGTCTGT ACTCATTTCC AGAGCCAGAA GCAGCTCAGC CCATGACAAA GAAGCGAAAA
    GTAGATGGTT TGAGCACAGA GGCAGAGCAG CCCTTCATTC CAGTAGAGGT GCTCGTAGAC
    CTGTTCCTCA AGGAAGGTGC CTGTGATGAA TTGTTCTCCT ACCTCATTGA GAAAGTGAAG
    CGAAAGAAAA ATGTACTACG CCTGTGCTGT AAGAAGCTGA AGATTTTTGC AATGCCCATG
    CAGGATATCA AGATGATCCT GAAAATGGTG CAGCTGGACT CTATTGAAGA TTTGGAAGTG
    ACTTGTACCT GGAAGCTACC CACCTTGGCG AAATTTTCTC CTTACCTGGG CCAGATGATT
    AATCTGCGTA GACTCCTCCT CTCCCACATC CATGCATCTT CCTACATTTC CCCGGAGAAG
    GAAGAGCAGT ATATCGCCCA GTTCACCTCT CAGTTCCTCA GTCTGCAGTG CCTGCAGGCT
    CTCTATGTGG ACTCTTTATT TTTCCTTAGA GGCCGCCTGG ATCAGTTGCT CAGGCACGTG
    ATGAACCCCT TGGAAACCCT CTCAATAACT AACTGCCGGC TTTCGGAAGG GGATGTGATG
    CATCTGTCCC AGAGTCCCAG CGTCAGTCAG CTAAGTGTCC TGAGTCTAAG TGGGGTCATG
    CTGACCGATG TAAGTCCCGA GCCCCTCCAA GCTCTGCTGG AGAGAGCCTC TGCCACCCTC
    CAGGACCTGG TCTTTGATGA GTGTGGGATC ACGGATGATC AGCTCCTTGC CCTCCTGCCT
    TCCCTGAGCC ACTGCTCCCA GCTTACGACC TTAAGCTTCT ACGGGAATTC CATCTCCATA
    TCTGCCCTGC AGAGTCTCCT GCAGCACCTC ATCGGGCTGA GCAATCTGAC CCACGTGCTG
    TATCCTGTCC CCCTGGAGAG TTATGAGGAC ATCCATGGTA CCCTCCACCT GGAGAGGCTT
    GCCTATCTGC ATGCCAGGCT CAGGGAGTTG CTGTGTGAGT TGGGGCGGCC CAGCATGGTC
    TGGCTTAGTG CCAACCCCTG TCCTCACTGT GGGGACAGAA CCTTCTATGA CCCGGAGCCC
    ATCCTGTGCC CCTGTTTCAT GCCTAATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001291719.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1 and BC022008.1. On May 31, 2019 this sequence version replaced NM_001291719.1. Transcript Variant: This variant (9) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon, compared to variant 2. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 9-11 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.70113.1, SRR5189667.150869.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278648.1
    CDS116..1597
    Translation

    Target ORF information:

    RefSeq Version NM_001291719.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291719.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001318126.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-19
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK316395.1, BC022008.1 and AI613210.1. Transcript Variant: This variant (10) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon compared to variant 2. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Variants 9-11 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK316395.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305055.1
    CDS119..1600
    Translation

    Target ORF information:

    RefSeq Version NM_001318126.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318126.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001291719.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC395455.1, AK129783.1, BC022008.1 and AI613210.1. Transcript Variant: This variant (9) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon, compared to variant 2. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 9-11 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.299464.1, SRR5189661.70113.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278648.1
    CDS238..1719
    Translation

    Target ORF information:

    RefSeq Version NM_001291719.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291719.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001291717.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1, DB024053.1 and AK304593.1. On May 31, 2019 this sequence version replaced NM_001291717.1. Transcript Variant: This variant (8) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon, compared to variant 2. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR5189667.69748.1, SRR5189661.72042.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278646.1
    CDS359..1840
    Translation

    Target ORF information:

    RefSeq Version NM_001291717.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291717.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001318127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-20
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK302188.1, AC246793.1, BC022008.1 and AI613210.1. On or before Dec 24, 2015 this sequence version replaced XM_011530035.1, XM_011546898.1. Transcript Variant: This variant (11) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon compared to variant 2. The encoded isoform (b) has a shorter N-terminus compared to isoform a. Variants 9-11 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302188.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001305056.1
    CDS381..1862
    Translation

    Target ORF information:

    RefSeq Version NM_001318127.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001318127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version NM_001291717.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022008.1, DB024053.1, AK304593.1 and AI613210.1. Transcript Variant: This variant (8) differs in the 5' UTR, lacks part of the 5' coding region, and uses a downstream start codon, compared to variant 2. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AK302188.1, AK129783.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001278646.1
    CDS455..1936
    Translation

    Target ORF information:

    RefSeq Version NM_001291717.1
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001291717.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011530034.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528336.1
    Translation

    Target ORF information:

    RefSeq Version XM_011530034.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011530034.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011530034.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528336.1
    CDS184..1665
    Translation

    Target ORF information:

    RefSeq Version XM_011530034.3
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530034.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011530034.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011530034.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528336.1
    CDS655..2136
    Translation

    Target ORF information:

    RefSeq Version XM_011530034.2
    Organism Homo sapiens(human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530034.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011530035.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011528337.1
    Translation

    Target ORF information:

    RefSeq Version XM_011530035.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011530035.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011546898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011545200.1
    Translation

    Target ORF information:

    RefSeq Version XM_011546898.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011546898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17036
    Clone ID Related Accession (Same CDS sequence) NM_001291719.2 , NM_001318126.1 , XM_011530034.1 , XM_011530034.3 , NM_001291719.1 , XM_011530034.2 , XM_011530035.1 , NM_001291717.2 , NM_001318127.1 , NM_001291717.1 , XM_011546898.1 , XM_011546897.1
    Accession Version XM_011546897.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product melanoma antigen preferentially expressed in tumors isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011545199.1
    Translation

    Target ORF information:

    RefSeq Version XM_011546897.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens preferentially expressed antigen in melanoma (PRAME), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011546897.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAGTGTGT GGACAAGCCC ACGGAGACTT GTGGAGCTGG CAGGGCAGAG CCTGCTGAAG 
    GATGAGGCCC TGGCCATTGC CGCCCTGGAG TTGCTGCCCA GGGAGCTCTT CCCGCCACTC
    TTCATGGCAG CCTTTGACGG GAGACACAGC CAGACCCTGA AGGCAATGGT GCAGGCCTGG
    CCCTTCACCT GCCTCCCTCT GGGAGTGCTG ATGAAGGGAC AACATCTTCA CCTGGAGACC
    TTCAAAGCTG TGCTTGATGG ACTTGATGTG CTCCTTGCCC AGGAGGTTCG CCCCAGGAGG
    TGGAAACTTC AAGTGCTGGA TTTACGGAAG AACTCTCATC AGGACTTCTG GACTGTATGG
    TCTGGAAACA GGGCCAGTCT GTACTCATTT CCAGAGCCAG AAGCAGCTCA GCCCATGACA
    AAGAAGCGAA AAGTAGATGG TTTGAGCACA GAGGCAGAGC AGCCCTTCAT TCCAGTAGAG
    GTGCTCGTAG ACCTGTTCCT CAAGGAAGGT GCCTGTGATG AATTGTTCTC CTACCTCATT
    GAGAAAGTGA AGCGAAAGAA AAATGTACTA CGCCTGTGCT GTAAGAAGCT GAAGATTTTT
    GCAATGCCCA TGCAGGATAT CAAGATGATC CTGAAAATGG TGCAGCTGGA CTCTATTGAA
    GATTTGGAAG TGACTTGTAC CTGGAAGCTA CCCACCTTGG CGAAATTTTC TCCTTACCTG
    GGCCAGATGA TTAATCTGCG TAGACTCCTC CTCTCCCACA TCCATGCATC TTCCTACATT
    TCCCCGGAGA AGGAAGAGCA GTATATCGCC CAGTTCACCT CTCAGTTCCT CAGTCTGCAG
    TGCCTGCAGG CTCTCTATGT GGACTCTTTA TTTTTCCTTA GAGGCCGCCT GGATCAGTTG
    CTCAGGCACG TGATGAACCC CTTGGAAACC CTCTCAATAA CTAACTGCCG GCTTTCGGAA
    GGGGATGTGA TGCATCTGTC CCAGAGTCCC AGCGTCAGTC AGCTAAGTGT CCTGAGTCTA
    AGTGGGGTCA TGCTGACCGA TGTAAGTCCC GAGCCCCTCC AAGCTCTGCT GGAGAGAGCC
    TCTGCCACCC TCCAGGACCT GGTCTTTGAT GAGTGTGGGA TCACGGATGA TCAGCTCCTT
    GCCCTCCTGC CTTCCCTGAG CCACTGCTCC CAGCTTACGA CCTTAAGCTT CTACGGGAAT
    TCCATCTCCA TATCTGCCCT GCAGAGTCTC CTGCAGCACC TCATCGGGCT GAGCAATCTG
    ACCCACGTGC TGTATCCTGT CCCCCTGGAG AGTTATGAGG ACATCCATGG TACCCTCCAC
    CTGGAGAGGC TTGCCTATCT GCATGCCAGG CTCAGGGAGT TGCTGTGTGA GTTGGGGCGG
    CCCAGCATGG TCTGGCTTAG TGCCAACCCC TGTCCTCACT GTGGGGACAG AACCTTCTAT
    GACCCGGAGC CCATCCTGTG CCCCTGTTTC ATGCCTAATT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.