MED19 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED19
Entrez Gene ID	219541
Full Name	mediator complex subunit 19
Synonyms	DT2P1G7,LCMR1,MED19AS
General protein information	Preferred Names mediator complex subunit 19 Names mediator of RNA polymerase II transcription subunit 19 lung cancer metastasis-related protein 1 mediator of RNA polymerase II transcription, subunit 19 homolog putative mediator subunit MED19AS protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q12.1
Summary	The protein encoded by this gene is a subunit of the Mediator complex, which binds to gene-specific regulatory factors and provides support for the basal RNA polymerase II transcription machinery. This gene has been implicated in the growth of several types of cancer, and inhibition of its expression inhibits the growth and spread of these cancers. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015].
Disorder MIM:	612385

mRNA and Protein(s)

mRNA	Protein	Name
NM_153450.3	NP_703151.3	mediator of RNA polymerase II transcription subunit 19 isoform 2
NM_153450.4	NP_703151.3	mediator of RNA polymerase II transcription subunit 19 isoform 2
NM_001317078.1	NP_001304007.1	mediator of RNA polymerase II transcription subunit 19 isoform 1
NM_153450.2	NP_703151.2	mediator of RNA polymerase II transcription subunit 19 isoform 2
NM_153450.1	NP_703151.1	mediator of RNA polymerase II transcription subunit 19
NM_001317078.3	NP_001304007.2	mediator of RNA polymerase II transcription subunit 19 isoform 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MED19	XP_426411.2
	MED19	XP_001101030.2
Canis lupus familiaris (dog)	MED19	XP_852656.2
Mus musculus (house mouse)	Med19	NP_080161.1
Bos taurus (cattle)	MED19	NP_001098818.1
Homo sapiens (human)	MED19	NP_703151.1
Pan troglodytes (chimpanzee)	MED19	XP_508439.3
Rattus norvegicus (Norway rat)	Med19	NP_001101211.1
Danio rerio (zebrafish)	med19b	NP_001003416.2
Danio rerio (zebrafish)	med19a	NP_001070059.1
Xenopus tropicalis (tropical clawed frog)	med19	NP_001095276.1

Gene Ontology
Bibliography

Related articles in PubMed

Knockdown of mediator subunit Med19 suppresses bladder cancer cell proliferation and migration by downregulating Wnt/?-catenin signalling pathway.
Yuan H, Yu S, Cui Y, Men C, Yang D, Gao Z, Zhu Z, Wu J
Journal of cellular and molecular medicine21(12)3254-3263(2017 Dec)

LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells.
Xu Y, Liang Z, Li C, Yang Z, Chen L
Molecular medicine reports16(4)4159-4164(2017 Oct)

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells.
Yu S, Wang Y, Yuan H, Zhao H, Lv W, Chen J, Wan F, Liu D, Gao Z, Wu J
PloS one12(1)e0171134(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Med19 promotes breast cancer cell proliferation and that this effect is associated with regulating CBFA2T3 and HEB expression.
Title: Med19 promotes breast cancer cell proliferation by regulating CBFA2T3/HEB expression.

LCMR1 modulation was sufficient to positively regulate endogenous Tspan8 expression, with concomitant in vitro phenotypic changes such as loss of melanoma cell-matrix adherence and increase in invasion, and Tspan8 expression promoted tumourigenicity in vivo.
Title: A large-scale RNAi screen identifies LCMR1 as a critical regulator of Tspan8-mediated melanoma invasion.

We knocked down the Med19 expression in prostate cancer cell lines LNCaP and PC3 by using lentivirus siRNA. Cell proliferation, anchor-independent growth, migration, and invasion were suppressed in Med19 knockdown prostate cancer cells.
Title: Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells.

FOXD3/miR-214/MED19 axis is important for the regulation of growth, invasion and metastasis of colorectal cancer
Title: The FOXD3/miR-214/MED19 axis suppresses tumour growth and metastasis in human colorectal cancer.

High expression of Med19 is associated with cisplatin resistance in non-small cell lung cancer cells.
Title: Knockdown of Med19 suppresses proliferation and enhances chemo-sensitivity to cisplatin in non-small cell lung cancer cells.

The following MED19 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED19 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07037	NM_153450.4 Latest version!	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu45198	NM_001317078.1 Latest version!	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu109309	NM_001317078.3 Latest version!	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu82769	NM_153450.2 Latest version!	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu07037	NM_153450.3	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07037	NM_153450.1	Homo sapiens mediator complex subunit 19 (MED19), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED19 ( NM_153450.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_703151.3 Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07037
Clone ID Related Accession (Same CDS sequence)	NM_153450.3 , NM_153450.4 , NM_153450.1
Accession Version	NM_153450.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 19 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB457116.1, BC037223.1 and AA972146.1. On Jul 28, 2018 this sequence version replaced NM_153450.2. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037223.1, SRR1803617.461603.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703151.3
CDS	37..621
Translation	MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS

Target ORF information:

RefSeq Version	NM_153450.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153450.3

ORF Insert Sequence:

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_153450.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_703151.3 Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07037
Clone ID Related Accession (Same CDS sequence)	NM_153450.3 , NM_153450.4 , NM_153450.1
Accession Version	NM_153450.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 19 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB457116.1, BC037223.1 and AA972146.1. On Jun 2, 2019 this sequence version replaced NM_153450.3. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037223.1, BI754118.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703151.3
CDS	37..621
Translation	MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS

Target ORF information:

RefSeq Version	NM_153450.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153450.4

ORF Insert Sequence:

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_153450.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_703151.1 Homo sapiens mediator complex subunit 19 (MED19), mRNA.

Price & Availability↑

CloneID	OHu07037
Clone ID Related Accession (Same CDS sequence)	NM_153450.3 , NM_153450.4 , NM_153450.1
Accession Version	NM_153450.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-20
Organism	Homo sapiens(Human)
Product	mediator of RNA polymerase II transcription subunit 19
Comment

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703151.1
Translation	MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS

Target ORF information:

RefSeq Version	NM_153450.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153450.1

ORF Insert Sequence:

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GGTAAACCCA GTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_001317078.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304007.1 Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu45198
Clone ID Related Accession (Same CDS sequence)	NM_001317078.1
Accession Version	NM_001317078.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 19 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB457116.1, AP000662.4, AY148462.1, EB386171.1, BC037223.1 and AA972146.1. On Nov 13, 2015 this sequence version replaced XM_005273821.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.31251.1, SRR1803617.411301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAAATTA CCAACGGCAG ACACGGAGAC AGCGCCGGGG CGGAGGGTAC GATGGAGAAT 
TTCACGGCAC TGTTTGGGGC TCAGGCTGAC CCACCACCGC CCCCAACCGC ACTCGGCTTC 
GGACCAGGAA AGCCTCCACC CCCGCCACCG CCTCCTGCGG GCGGGGGACC CGGCACGGCC 
CCGCCTCCCA CCGCGGCCAC GGCTCCTCCT GGCGCGGACA AGTCAGGAGC TGGCTGTGGC 
CCTTTTTACC TCATGAGGGA ACTGCCAGGT AGCACAGAGC TGACAGGCAG CACGAATCTG 
ATCACACACT ACAACTTGGA ACAAGCCTAT AATAAATTCT GTGGGAAGAA GGTGAAGGAG 
AAGCTAAGTA ACTTCCTGCC TGACCTGCCA GGGATGATTG ATCTGCCTGG TTCCCATGAT 
AACAGCAGCC TCCGCTCTCT CATTGAGAAG CCCCCTATTC TCAGTAGCTC TTTCAATCCT 
ATCACAGGGA CCATGCTGGC CGGCTTCCGC CTCCACACTG GCCCGTTGCC GGAGCAGTGT 
CGTCTGATGC ATATTCAGCC TCCCAAGAAG AAGAATAAGC ACAAGCACAA ACAGAGCCGT 
ACCCAGGATC CTGTCCCCCC AGAAACACCA TCTGATTCAG ATCACAAGAA GAAGAAAAAG 
AAAAAAGAAG AGGATCCTGA ACGGAAAAGG AAGAAGAAAG AGAAGAAGAA AAAGAAGAAT 
CGACATAGTC CAGACCACCC AGGTATGGGC AGCTCCCAGG CCAGCAGCAG CAGCAGCCTA 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304007.1
CDS	94..879
Translation	MKITNGRHGDSAGAEGTMENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPETPSDSDHKKKKKKKEEDPERKRKKKEKKKKKNRHSPDHPGMGSSQASSSSSLR

Target ORF information:

RefSeq Version	NM_001317078.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317078.1

ORF Insert Sequence:

ATGAAAATTA CCAACGGCAG ACACGGAGAC AGCGCCGGGG CGGAGGGTAC GATGGAGAAT 
TTCACGGCAC TGTTTGGGGC TCAGGCTGAC CCACCACCGC CCCCAACCGC ACTCGGCTTC 
GGACCAGGAA AGCCTCCACC CCCGCCACCG CCTCCTGCGG GCGGGGGACC CGGCACGGCC 
CCGCCTCCCA CCGCGGCCAC GGCTCCTCCT GGCGCGGACA AGTCAGGAGC TGGCTGTGGC 
CCTTTTTACC TCATGAGGGA ACTGCCAGGT AGCACAGAGC TGACAGGCAG CACGAATCTG 
ATCACACACT ACAACTTGGA ACAAGCCTAT AATAAATTCT GTGGGAAGAA GGTGAAGGAG 
AAGCTAAGTA ACTTCCTGCC TGACCTGCCA GGGATGATTG ATCTGCCTGG TTCCCATGAT 
AACAGCAGCC TCCGCTCTCT CATTGAGAAG CCCCCTATTC TCAGTAGCTC TTTCAATCCT 
ATCACAGGGA CCATGCTGGC CGGCTTCCGC CTCCACACTG GCCCGTTGCC GGAGCAGTGT 
CGTCTGATGC ATATTCAGCC TCCCAAGAAG AAGAATAAGC ACAAGCACAA ACAGAGCCGT 
ACCCAGGATC CTGTCCCCCC AGAAACACCA TCTGATTCAG ATCACAAGAA GAAGAAAAAG 
AAAAAAGAAG AGGATCCTGA ACGGAAAAGG AAGAAGAAAG AGAAGAAGAA AAAGAAGAAT 
CGACATAGTC CAGACCACCC AGGTATGGGC AGCTCCCAGG CCAGCAGCAG CAGCAGCCTA 
CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_001317078.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001304007.2 Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu109309
Clone ID Related Accession (Same CDS sequence)	NM_001317078.3
Accession Version	NM_001317078.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-11
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 19 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000662.4, AY148462.1 and U92995.1. On Dec 2, 2019 this sequence version replaced NM_001317078.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GAAACACCAT CTGATTCAGA TCACAAGAAG 
AAGAAAAAGA AAAAAGAAGA GGATCCTGAA CGGAAAAGGA AGAAGAAAGA GAAGAAGAAA 
AAGAAGAATC GACATAGTCC AGACCACCCA GGTATGGGCA GCTCCCAGGC CAGCAGCAGC 
AGCAGCCTAC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001304007.2
CDS	145..879
Translation	MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPETPSDSDHKKKKKKKEEDPERKRKKKEKKKKKNRHSPDHPGMGSSQASSSSSLR

Target ORF information:

RefSeq Version	NM_001317078.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001317078.3

ORF Insert Sequence:

ATGGAGAATT TCACGGCACT GTTTGGGGCT CAGGCTGACC CACCACCGCC CCCAACCGCA 
CTCGGCTTCG GACCAGGAAA GCCTCCACCC CCGCCACCGC CTCCTGCGGG CGGGGGACCC 
GGCACGGCCC CGCCTCCCAC CGCGGCCACG GCTCCTCCTG GCGCGGACAA GTCAGGAGCT 
GGCTGTGGCC CTTTTTACCT CATGAGGGAA CTGCCAGGTA GCACAGAGCT GACAGGCAGC 
ACGAATCTGA TCACACACTA CAACTTGGAA CAAGCCTATA ATAAATTCTG TGGGAAGAAG 
GTGAAGGAGA AGCTAAGTAA CTTCCTGCCT GACCTGCCAG GGATGATTGA TCTGCCTGGT 
TCCCATGATA ACAGCAGCCT CCGCTCTCTC ATTGAGAAGC CCCCTATTCT CAGTAGCTCT 
TTCAATCCTA TCACAGGGAC CATGCTGGCC GGCTTCCGCC TCCACACTGG CCCGTTGCCG 
GAGCAGTGTC GTCTGATGCA TATTCAGCCT CCCAAGAAGA AGAATAAGCA CAAGCACAAA 
CAGAGCCGTA CCCAGGATCC TGTCCCCCCA GAAACACCAT CTGATTCAGA TCACAAGAAG 
AAGAAAAAGA AAAAAGAAGA GGATCCTGAA CGGAAAAGGA AGAAGAAAGA GAAGAAGAAA 
AAGAAGAATC GACATAGTCC AGACCACCCA GGTATGGGCA GCTCCCAGGC CAGCAGCAGC 
AGCAGCCTAC GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED19 ( NM_153450.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_703151.2 Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

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CloneID	OHu82769
Clone ID Related Accession (Same CDS sequence)	NM_153450.2
Accession Version	NM_153450.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 19 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB457116.1, BC037223.1 and AA972146.1. On Nov 13, 2015 this sequence version replaced NM_153450.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by the available transcript and conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037223.1, SRR1803617.461603.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATTA CCAACGGCAG ACACGGAGAC AGCGCCGGGG CGGAGGGTAC GATGGAGAAT 
TTCACGGCAC TGTTTGGGGC TCAGGCTGAC CCACCACCGC CCCCAACCGC ACTCGGCTTC 
GGACCAGGAA AGCCTCCACC CCCGCCACCG CCTCCTGCGG GCGGGGGACC CGGCACGGCC 
CCGCCTCCCA CCGCGGCCAC GGCTCCTCCT GGCGCGGACA AGTCAGGAGC TGGCTGTGGC 
CCTTTTTACC TCATGAGGGA ACTGCCAGGT AGCACAGAGC TGACAGGCAG CACGAATCTG 
ATCACACACT ACAACTTGGA ACAAGCCTAT AATAAATTCT GTGGGAAGAA GGTGAAGGAG 
AAGCTAAGTA ACTTCCTGCC TGACCTGCCA GGGATGATTG ATCTGCCTGG TTCCCATGAT 
AACAGCAGCC TCCGCTCTCT CATTGAGAAG CCCCCTATTC TCAGTAGCTC TTTCAATCCT 
ATCACAGGGA CCATGCTGGC CGGCTTCCGC CTCCACACTG GCCCGTTGCC GGAGCAGTGT 
CGTCTGATGC ATATTCAGCC TCCCAAGAAG AAGAATAAGC ACAAGCACAA ACAGAGCCGT 
ACCCAGGATC CTGTCCCCCC AGGTAAACCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_703151.2
CDS	94..729
Translation	MKITNGRHGDSAGAEGTMENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS

Target ORF information:

RefSeq Version	NM_153450.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 19 (MED19), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153450.2

ORF Insert Sequence:

ATGAAAATTA CCAACGGCAG ACACGGAGAC AGCGCCGGGG CGGAGGGTAC GATGGAGAAT 
TTCACGGCAC TGTTTGGGGC TCAGGCTGAC CCACCACCGC CCCCAACCGC ACTCGGCTTC 
GGACCAGGAA AGCCTCCACC CCCGCCACCG CCTCCTGCGG GCGGGGGACC CGGCACGGCC 
CCGCCTCCCA CCGCGGCCAC GGCTCCTCCT GGCGCGGACA AGTCAGGAGC TGGCTGTGGC 
CCTTTTTACC TCATGAGGGA ACTGCCAGGT AGCACAGAGC TGACAGGCAG CACGAATCTG 
ATCACACACT ACAACTTGGA ACAAGCCTAT AATAAATTCT GTGGGAAGAA GGTGAAGGAG 
AAGCTAAGTA ACTTCCTGCC TGACCTGCCA GGGATGATTG ATCTGCCTGG TTCCCATGAT 
AACAGCAGCC TCCGCTCTCT CATTGAGAAG CCCCCTATTC TCAGTAGCTC TTTCAATCCT 
ATCACAGGGA CCATGCTGGC CGGCTTCCGC CTCCACACTG GCCCGTTGCC GGAGCAGTGT 
CGTCTGATGC ATATTCAGCC TCCCAAGAAG AAGAATAAGC ACAAGCACAA ACAGAGCCGT 
ACCCAGGATC CTGTCCCCCC AGGTAAACCC AGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knockdown of mediator subunit Med19 suppresses bladder cancer cell proliferation and migration by downregulating Wnt/?-catenin signalling pathway.
Journal of cellular and molecular medicine21(12)3254-3263(2017 Dec)
Yuan H,Yu S,Cui Y,Men C,Yang D,Gao Z,Zhu Z,Wu J

LCMR1 interacts with DEK to suppress apoptosis in lung cancer cells.
Molecular medicine reports16(4)4159-4164(2017 Oct)
Xu Y,Liang Z,Li C,Yang Z,Chen L

Knockdown of Mediator Complex Subunit 19 Suppresses the Growth and Invasion of Prostate Cancer Cells.
PloS one12(1)e0171134(2017)
Yu S,Wang Y,Yuan H,Zhao H,Lv W,Chen J,Wan F,Liu D,Gao Z,Wu J

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MED19 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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