ERH cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ERH
Entrez Gene ID	2079
Full Name	ERH, mRNA splicing and mitosis factor
Synonyms	DROER
General protein information	Preferred Names ERH, mRNA splicing and mitosis factor Names enhancer of rudimentary homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q24.1
Disorder MIM:	601191

mRNA and Protein(s)

mRNA	Protein	Name
NM_004450.3	NP_004441.1	enhancer of rudimentary homolog
NM_004450.2	NP_004441.1	enhancer of rudimentary homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	ERH	NP_004441.1
Canis lupus familiaris (dog)	ERH	XP_537493.4
Drosophila melanogaster (fruit fly)	e(r)	NP_001259363.1
Mus musculus (house mouse)	Erh	NP_031977.1
Gallus gallus (chicken)	ERH	NP_001006475.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000516	XP_310576.1
Bos taurus (cattle)	ERH	NP_001029508.1
Caenorhabditis elegans (roundworm)	T21C9.4	NP_505713.1
Arabidopsis thaliana (thale cress)	ER	NP_568240.1
Oryza sativa (rice)	Os02g0611450	NP_001173070.1
Pan troglodytes (chimpanzee)	ERH	XP_003952576.1
	ERH	XP_001109334.1
Rattus norvegicus (Norway rat)	Erh	NP_001102912.1
Danio rerio (zebrafish)	erh	NP_571303.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000515	XP_001237270.1
Xenopus tropicalis (tropical clawed frog)	erh	XP_002935377.1

Gene Ontology
Bibliography

Related articles in PubMed

Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.
Drakouli S, Lyberopoulou A, Papathanassiou M, Mylonis I, Georgatsou E
The FEBS journal284(15)2482-2500(2017 Aug)

Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.
Kavanaugh G, Zhao R, Guo Y, Mohni KN, Glick G, Lacy ME, Hutson MS, Ascano M, Cortez D
Molecular and cellular biology35(17)2979-90(2015 Sep)

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Takai H, Masuda K, Sato T, Sakaguchi Y, Suzuki T, Suzuki T, Koyama-Nasu R, Nasu-Nishimura Y, Katou Y, Ogawa H, Morishita Y, Kozuka-Hata H, Oyama M, Todo T, Ino Y, Mukasa A, Saito N, Toyoshima C, Shirahige K, Akiyama T
Cell reports9(1)48-60(2014 Oct)

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer.
Weng MT, Luo J
Protein & cell4(11)807-12(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that ERH interacts directly in nucleus with C-terminal Arg-Gly-rich region of SAFB1/SAFB2 and this multimer co-localizes in insoluble nuclear fraction; binding of ERH reverses inhibition exerted by SAFB1/SAFB2 on SRPK1. (ERH = enhancer of rudimentary homolog protein; SAFB = scaffold attachment factor B; SRPK1 = splicing kinase SR protein kinase-1)
Title: Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.

ERH regulation of RNA processing is needed to ensure faithful DNA replication and repair.
Title: Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.

REVIEW: recent findings indicate that ERH plays an important role in cell cycle through its mRNA splicing activity and is critically required for genomic stability and cancer cell survival
Title: The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer.

Identification of amino acid residues of ERH required for its recruitment to nuclear speckles and replication foci
Title: Identification of amino acid residues of ERH required for its recruitment to nuclear speckles and replication foci in HeLa cells.

Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells.
Title: Evolutionarily conserved protein ERH controls CENP-E mRNA splicing and is required for the survival of KRAS mutant cancer cells.

The following ERH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ERH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14942	NM_004450.3 Latest version!	Homo sapiens ERH mRNA splicing and mitosis factor (ERH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14942	NM_004450.2	Homo sapiens ERH, mRNA splicing and mitosis factor (ERH), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ERH ( NM_004450.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004441.1 Homo sapiens ERH mRNA splicing and mitosis factor (ERH), mRNA.

Price & Availability↑

CloneID	OHu14942
Clone ID Related Accession (Same CDS sequence)	NM_004450.3 , NM_004450.2
Accession Version	NM_004450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	enhancer of rudimentary homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U66871.1 and AK130850.1. On Nov 22, 2018 this sequence version replaced NM_004450.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.587541.1, SRR1163655.620782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000557016.6/ ENSP00000451080.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTCACA CCATTTTGCT GGTACAGCCT ACCAAGAGGC CAGAAGGCAG AACTTATGCT 
GACTACGAAT CTGTGAATGA ATGCATGGAA GGTGTTTGTA AAATGTATGA AGAACATCTG 
AAAAGAATGA ATCCCAACAG TCCCTCTATC ACATATGACA TCAGTCAGTT GTTTGATTTC 
ATCGATGATC TGGCAGACCT CAGCTGCCTG GTTTACCGAG CTGATACCCA GACATACCAG 
CCTTATAACA AAGACTGGAT TAAAGAGAAG ATCTACGTGC TCCTTCGTCG GCAGGCCCAA 
CAGGCTGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004441.1
CDS	67..381
Translation	MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK

Target ORF information:

RefSeq Version	NM_004450.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ERH mRNA splicing and mitosis factor (ERH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004450.3

ORF Insert Sequence:

ATGTCTCACA CCATTTTGCT GGTACAGCCT ACCAAGAGGC CAGAAGGCAG AACTTATGCT 
GACTACGAAT CTGTGAATGA ATGCATGGAA GGTGTTTGTA AAATGTATGA AGAACATCTG 
AAAAGAATGA ATCCCAACAG TCCCTCTATC ACATATGACA TCAGTCAGTT GTTTGATTTC 
ATCGATGATC TGGCAGACCT CAGCTGCCTG GTTTACCGAG CTGATACCCA GACATACCAG 
CCTTATAACA AAGACTGGAT TAAAGAGAAG ATCTACGTGC TCCTTCGTCG GCAGGCCCAA 
CAGGCTGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ERH ( NM_004450.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004441.1 Homo sapiens ERH, mRNA splicing and mitosis factor (ERH), mRNA.

Price & Availability↑

CloneID	OHu14942
Clone ID Related Accession (Same CDS sequence)	NM_004450.3 , NM_004450.2
Accession Version	NM_004450.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	enhancer of rudimentary homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U66871.1 and AK130850.1. On Jul 10, 2008 this sequence version replaced NM_004450.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.532772.1, SRR1163655.620782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCACA CCATTTTGCT GGTACAGCCT ACCAAGAGGC CAGAAGGCAG AACTTATGCT 
GACTACGAAT CTGTGAATGA ATGCATGGAA GGTGTTTGTA AAATGTATGA AGAACATCTG 
AAAAGAATGA ATCCCAACAG TCCCTCTATC ACATATGACA TCAGTCAGTT GTTTGATTTC 
ATCGATGATC TGGCAGACCT CAGCTGCCTG GTTTACCGAG CTGATACCCA GACATACCAG 
CCTTATAACA AAGACTGGAT TAAAGAGAAG ATCTACGTGC TCCTTCGTCG GCAGGCCCAA 
CAGGCTGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004441.1
CDS	72..386
Translation	MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDLADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK

Target ORF information:

RefSeq Version	NM_004450.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ERH, mRNA splicing and mitosis factor (ERH), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004450.2

ORF Insert Sequence:

ATGTCTCACA CCATTTTGCT GGTACAGCCT ACCAAGAGGC CAGAAGGCAG AACTTATGCT 
GACTACGAAT CTGTGAATGA ATGCATGGAA GGTGTTTGTA AAATGTATGA AGAACATCTG 
AAAAGAATGA ATCCCAACAG TCCCTCTATC ACATATGACA TCAGTCAGTT GTTTGATTTC 
ATCGATGATC TGGCAGACCT CAGCTGCCTG GTTTACCGAG CTGATACCCA GACATACCAG 
CCTTATAACA AAGACTGGAT TAAAGAGAAG ATCTACGTGC TCCTTCGTCG GCAGGCCCAA 
CAGGCTGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enhancer of rudimentary homologue interacts with scaffold attachment factor B at the nuclear matrix to regulate SR protein phosphorylation.
The FEBS journal284(15)2482-2500(2017 Aug)
Drakouli S,Lyberopoulou A,Papathanassiou M,Mylonis I,Georgatsou E

Enhancer of Rudimentary Homolog Affects the Replication Stress Response through Regulation of RNA Processing.
Molecular and cellular biology35(17)2979-90(2015 Sep)
Kavanaugh G,Zhao R,Guo Y,Mohni KN,Glick G,Lacy ME,Hutson MS,Ascano M,Cortez D

5-Hydroxymethylcytosine plays a critical role in glioblastomagenesis by recruiting the CHTOP-methylosome complex.
Cell reports9(1)48-60(2014 Oct)
Takai H,Masuda K,Sato T,Sakaguchi Y,Suzuki T,Suzuki T,Koyama-Nasu R,Nasu-Nishimura Y,Katou Y,Ogawa H,Morishita Y,Kozuka-Hata H,Oyama M,Todo T,Ino Y,Mukasa A,Saito N,Toyoshima C,Shirahige K,Akiyama T

The enigmatic ERH protein: its role in cell cycle, RNA splicing and cancer.
Protein & cell4(11)807-12(2013 Nov)
Weng MT,Luo J