EPHB4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPHB4
Entrez Gene ID	2050
Full Name	EPH receptor B4
Synonyms	HFASD,HTK,MYK1,TYRO11
General protein information	Preferred Names EPH receptor B4 Names ephrin type-B receptor 4 ephrin receptor EphB4 hepatoma transmembrane kinase soluble EPHB4 variant 1 soluble EPHB4 variant 2 soluble EPHB4 variant 3 tyrosine-protein kinase TYRO11 tyrosine-protein kinase receptor HTK
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development. [provided by RefSeq, Jul 2008].
Disorder MIM:	600011
Disorder Html:	{Hydrops fetalis, nonimmune, and/or atrial septal defect}, 617300 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004444.4	NP_004435.3	ephrin type-B receptor 4 precursor
NM_004444.5	NP_004435.3	ephrin type-B receptor 4 precursor
XM_017011816.1	XP_016867305.1	ephrin type-B receptor 4 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EPHB4	NP_004435.3
Canis lupus familiaris (dog)	EPHB4	XP_546948.3
Mus musculus (house mouse)	Ephb4	NP_001153043.1
Rattus norvegicus (Norway rat)	Ephb4	XP_003751205.1
	EPHB4	XP_002803271.1
Bos taurus (cattle)	EPHB4	NP_001193197.1
Danio rerio (zebrafish)	ephb4b	NP_571492.1
Pan troglodytes (chimpanzee)	EPHB4	XP_519269.3
Danio rerio (zebrafish)	ephb4a	NP_571489.1

Gene Ontology

The following EPHB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPHB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13402	NM_004444.5 Latest version!	Homo sapiens EPH receptor B4 (EPHB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu97077	XM_017011816.1 Latest version!	Homo sapiens EPH receptor B4 (EPHB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OHu13402	NM_004444.4	Homo sapiens EPH receptor B4 (EPHB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPHB4 ( NM_004444.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004435.3 Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Price & Availability↑

CloneID	OHu13402
Clone ID Related Accession (Same CDS sequence)	NM_004444.4 , NM_004444.5
Accession Version	NM_004444.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	ephrin type-B receptor 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB962032.1, BC052804.1 and AY056048.1. This sequence is a reference standard in the RefSeqGene project. On Nov 16, 2004 this sequence version replaced NM_004444.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052804.1, U07695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC 
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC 
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC 
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA 
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG 
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG 
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT 
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG 
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG 
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG 
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG 
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC 
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG 
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC 
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC 
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC 
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG 
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC 
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC 
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC 
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC 
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA 
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC 
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA 
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004435.3
CDS	492..3455
Translation	MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

Target ORF information:

RefSeq Version	NM_004444.4
Organism	Homo sapiens(human)
Definition	Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004444.4

ORF Insert Sequence:

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC 
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC 
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC 
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA 
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG 
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG 
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT 
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG 
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG 
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG 
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG 
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC 
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG 
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC 
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC 
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC 
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG 
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC 
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC 
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC 
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC 
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA 
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC 
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA 
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHB4 ( NM_004444.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004435.3 Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Price & Availability↑

CloneID	OHu13402
Clone ID Related Accession (Same CDS sequence)	NM_004444.4 , NM_004444.5
Accession Version	NM_004444.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2964bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-09
Organism	Homo sapiens(human)
Product	ephrin type-B receptor 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011895.4, CB962032.1, BC052804.1 and AY056048.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004444.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC052804.1, U07695.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358173.8/ ENSP00000350896.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC 
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC 
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC 
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA 
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG 
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG 
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT 
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG 
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG 
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG 
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG 
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC 
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG 
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC 
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC 
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC 
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG 
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC 
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC 
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC 
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC 
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA 
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC 
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA 
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004435.3
CDS	494..3457
Translation	MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

Target ORF information:

RefSeq Version	NM_004444.5
Organism	Homo sapiens(human)
Definition	Homo sapiens EPH receptor B4 (EPHB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004444.5

ORF Insert Sequence:

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTAC CTCCTGCAGT GTCTGACATC 
CGGGTGACGC GGTCCTCACC CAGCAGCTTG AGCCTGGCCT GGGCTGTTCC CCGGGCACCC 
AGTGGGGCTG TGCTGGACTA CGAGGTCAAA TACCATGAGA AGGGCGCCGA GGGTCCCAGC 
AGCGTGCGGT TCCTGAAGAC GTCAGAAAAC CGGGCAGAGC TGCGGGGGCT GAAGCGGGGA 
GCCAGCTACC TGGTGCAGGT ACGGGCGCGC TCTGAGGCCG GCTACGGGCC CTTCGGCCAG 
GAACATCACA GCCAGACCCA ACTGGATGAG AGCGAGGGCT GGCGGGAGCA GCTGGCCCTG 
ATTGCGGGCA CGGCAGTCGT GGGTGTGGTC CTGGTCCTGG TGGTCATTGT GGTCGCAGTT 
CTCTGCCTCA GGAAGCAGAG CAATGGGAGA GAAGCAGAAT ATTCGGACAA ACACGGACAG 
TATCTCATCG GACATGGTAC TAAGGTCTAC ATCGACCCCT TCACTTATGA AGACCCTAAT 
GAGGCTGTGA GGGAATTTGC AAAAGAGATC GATGTCTCCT ACGTCAAGAT TGAAGAGGTG 
ATTGGTGCAG GTGAGTTTGG CGAGGTGTGC CGGGGGCGGC TCAAGGCCCC AGGGAAGAAG 
GAGAGCTGTG TGGCAATCAA GACCCTGAAG GGTGGCTACA CGGAGCGGCA GCGGCGTGAG 
TTTCTGAGCG AGGCCTCCAT CATGGGCCAG TTCGAGCACC CCAATATCAT CCGCCTGGAG 
GGCGTGGTCA CCAACAGCAT GCCCGTCATG ATTCTCACAG AGTTCATGGA GAACGGCGCC 
CTGGACTCCT TCCTGCGGCT AAACGACGGA CAGTTCACAG TCATCCAGCT CGTGGGCATG 
CTGCGGGGCA TCGCCTCGGG CATGCGGTAC CTTGCCGAGA TGAGCTACGT CCACCGAGAC 
CTGGCTGCTC GCAACATCCT AGTCAACAGC AACCTCGTCT GCAAAGTGTC TGACTTTGGC 
CTTTCCCGAT TCCTGGAGGA GAACTCTTCC GATCCCACCT ACACGAGCTC CCTGGGAGGA 
AAGATTCCCA TCCGATGGAC TGCCCCGGAG GCCATTGCCT TCCGGAAGTT CACTTCCGCC 
AGTGATGCCT GGAGTTACGG GATTGTGATG TGGGAGGTGA TGTCATTTGG GGAGAGGCCG 
TACTGGGACA TGAGCAATCA GGACGTGATC AATGCCATTG AACAGGACTA CCGGCTGCCC 
CCGCCCCCAG ACTGTCCCAC CTCCCTCCAC CAGCTCATGC TGGACTGTTG GCAGAAAGAC 
CGGAATGCCC GGCCCCGCTT CCCCCAGGTG GTCAGCGCCC TGGACAAGAT GATCCGGAAC 
CCCGCCAGCC TCAAAATCGT GGCCCGGGAG AATGGCGGGG CCTCACACCC TCTCCTGGAC 
CAGCGGCAGC CTCACTACTC AGCTTTTGGC TCTGTGGGCG AGTGGCTTCG GGCCATCAAA 
ATGGGAAGAT ACGAAGAAAG TTTCGCAGCC GCTGGCTTTG GCTCCTTCGA GCTGGTCAGC 
CAGATCTCTG CTGAGGACCT GCTCCGAATC GGAGTCACTC TGGCGGGACA CCAGAAGAAA 
ATCTTGGCCA GTGTCCAGCA CATGAAGTCC CAGGCCAAGC CGGGAACCCC GGGTGGGACA 
GGAGGACCGG CCCCGCAGTA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPHB4 ( XM_017011816.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867305.1 Homo sapiens EPH receptor B4 (EPHB4), transcript variant X1, mRNA.

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CloneID	OHu97077
Clone ID Related Accession (Same CDS sequence)	XM_017011816.1
Accession Version	XM_017011816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ephrin type-B receptor 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTTA AGGGACAGAG CTACCATGCA 
CAAGGGTTCC CTGTGTCTCT GCCTCTCACA GTACCTCCTG CAGTGTCTGA CATCCGGGTG 
ACGCGGTCCT CACCCAGCAG CTTGAGCCTG GCCTGGGCTG TTCCCCGGGC ACCCAGTGGG 
GCTGTGCTGG ACTACGAGGT CAAATACCAT GAGAAGGGCG CCGAGGGTCC CAGCAGCGTG 
CGGTTCCTGA AGACGTCAGA AAACCGGGCA GAGCTGCGGG GGCTGAAGCG GGGAGCCAGC 
TACCTGGTGC AGGTACGGGC GCGCTCTGAG GCCGGCTACG GGCCCTTCGG CCAGGAACAT 
CACAGCCAGA CCCAACTGGA TGAGAGCGAG GGCTGGCGGG AGCAGCTGGC CCTGATTGCG 
GGCACGGCAG TCGTGGGTGT GGTCCTGGTC CTGGTGGTCA TTGTGGTCGC AGTTCTCTGC 
CTCAGGAAGC AGAGCAATGG GAGAGAAGCA GAATATTCGG ACAAACACGG ACAGTATCTC 
ATCGGACATG GTACTAAGGT CTACATCGAC CCCTTCACTT ATGAAGACCC TAATGAGGCT 
GTGAGGGAAT TTGCAAAAGA GATCGATGTC TCCTACGTCA AGATTGAAGA GGTGATTGGT 
GCAGGTGAGT TTGGCGAGGT GTGCCGGGGG CGGCTCAAGG CCCCAGGGAA GAAGGAGAGC 
TGTGTGGCAA TCAAGACCCT GAAGGGTGGC TACACGGAGC GGCAGCGGCG TGAGTTTCTG 
AGCGAGGCCT CCATCATGGG CCAGTTCGAG CACCCCAATA TCATCCGCCT GGAGGGCGTG 
GTCACCAACA GCATGCCCGT CATGATTCTC ACAGAGTTCA TGGAGAACGG CGCCCTGGAC 
TCCTTCCTGC GGCTAAACGA CGGACAGTTC ACAGTCATCC AGCTCGTGGG CATGCTGCGG 
GGCATCGCCT CGGGCATGCG GTACCTTGCC GAGATGAGCT ACGTCCACCG AGACCTGGCT 
GCTCGCAACA TCCTAGTCAA CAGCAACCTC GTCTGCAAAG TGTCTGACTT TGGCCTTTCC 
CGATTCCTGG AGGAGAACTC TTCCGATCCC ACCTACACGA GCTCCCTGGG AGGAAAGATT 
CCCATCCGAT GGACTGCCCC GGAGGCCATT GCCTTCCGGA AGTTCACTTC CGCCAGTGAT 
GCCTGGAGTT ACGGGATTGT GATGTGGGAG GTGATGTCAT TTGGGGAGAG GCCGTACTGG 
GACATGAGCA ATCAGGACGT GATCAATGCC ATTGAACAGG ACTACCGGCT GCCCCCGCCC 
CCAGACTGTC CCACCTCCCT CCACCAGCTC ATGCTGGACT GTTGGCAGAA AGACCGGAAT 
GCCCGGCCCC GCTTCCCCCA GGTGGTCAGC GCCCTGGACA AGATGATCCG GAACCCCGCC 
AGCCTCAAAA TCGTGGCCCG GGAGAATGGC GGGGCCTCAC ACCCTCTCCT GGACCAGCGG 
CAGCCTCACT ACTCAGCTTT TGGCTCTGTG GGCGAGTGGC TTCGGGCCAT CAAAATGGGA 
AGATACGAAG AAAGTTTCGC AGCCGCTGGC TTTGGCTCCT TCGAGCTGGT CAGCCAGATC 
TCTGCTGAGG ACCTGCTCCG AATCGGAGTC ACTCTGGCGG GACACCAGAA GAAAATCTTG 
GCCAGTGTCC AGCACATGAA GTCCCAGGCC AAGCCGGGAA CCCCGGGTGG GACAGGAGGA 
CCGGCCCCGC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867305.1
CDS	95..3112
Translation	MELRVLLCWASLAAALEETLLNTKLETADLKWVTFPQVDGQWEELSGLDEEQHSVRTYEVCDVQRAPGQAHWLRTGWVPRRGAVHVYATLRFTMLECLSLPRAGRSCKETFTVFYYESDADTATALTPAWMENPYIKVDTVAAEHLTRKRPGAEATGKVNVKTLRLGPLSKAGFYLAFQDQGACMALLSLHLFYKKCAQLTVNLTRFPETVPRELVVPVAGSCVVDAVPAPGPSPSLYCREDGQWAEQPVTGCSCAPGFEAAEGNTKCRACAQGTFKPLSGEGSCQPCPANSHSNTIGSAVCQCRVGYFRARTDPRGAPCTTPPSAPRSVVSRLNGSSLHLEWSAPLESGGREDLTYALRCRECRPGGSCAPCGGDLTFDPGPRDLVEPWVVVRGLRPDFTYTFEVTALNGVSSLATGPVPFEPVNVTTDREVKGQSYHAQGFPVSLPLTVPPAVSDIRVTRSSPSSLSLAWAVPRAPSGAVLDYEVKYHEKGAEGPSSVRFLKTSENRAELRGLKRGASYLVQVRARSEAGYGPFGQEHHSQTQLDESEGWREQLALIAGTAVVGVVLVLVVIVVAVLCLRKQSNGREAEYSDKHGQYLIGHGTKVYIDPFTYEDPNEAVREFAKEIDVSYVKIEEVIGAGEFGEVCRGRLKAPGKKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

Target ORF information:

RefSeq Version	XM_017011816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens EPH receptor B4 (EPHB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011816.1

ORF Insert Sequence:

ATGGAGCTCC GGGTGCTGCT CTGCTGGGCT TCGTTGGCCG CAGCTTTGGA AGAGACCCTG 
CTGAACACAA AATTGGAAAC TGCTGATCTG AAGTGGGTGA CATTCCCTCA GGTGGACGGG 
CAGTGGGAGG AACTGAGCGG CCTGGATGAG GAACAGCACA GCGTGCGCAC CTACGAAGTG 
TGTGACGTGC AGCGTGCCCC GGGCCAGGCC CACTGGCTTC GCACAGGTTG GGTCCCACGG 
CGGGGCGCCG TCCACGTGTA CGCCACGCTG CGCTTCACCA TGCTCGAGTG CCTGTCCCTG 
CCTCGGGCTG GGCGCTCCTG CAAGGAGACC TTCACCGTCT TCTACTATGA GAGCGATGCG 
GACACGGCCA CGGCCCTCAC GCCAGCCTGG ATGGAGAACC CCTACATCAA GGTGGACACG 
GTGGCCGCGG AGCATCTCAC CCGGAAGCGC CCTGGGGCCG AGGCCACCGG GAAGGTGAAT 
GTCAAGACGC TGCGTCTGGG ACCGCTCAGC AAGGCTGGCT TCTACCTGGC CTTCCAGGAC 
CAGGGTGCCT GCATGGCCCT GCTATCCCTG CACCTCTTCT ACAAAAAGTG CGCCCAGCTG 
ACTGTGAACC TGACTCGATT CCCGGAGACT GTGCCTCGGG AGCTGGTTGT GCCCGTGGCC 
GGTAGCTGCG TGGTGGATGC CGTCCCCGCC CCTGGCCCCA GCCCCAGCCT CTACTGCCGT 
GAGGATGGCC AGTGGGCCGA ACAGCCGGTC ACGGGCTGCA GCTGTGCTCC GGGGTTCGAG 
GCAGCTGAGG GGAACACCAA GTGCCGAGCC TGTGCCCAGG GCACCTTCAA GCCCCTGTCA 
GGAGAAGGGT CCTGCCAGCC ATGCCCAGCC AATAGCCACT CTAACACCAT TGGATCAGCC 
GTCTGCCAGT GCCGCGTCGG GTACTTCCGG GCACGCACAG ACCCCCGGGG TGCACCCTGC 
ACCACCCCTC CTTCGGCTCC GCGGAGCGTG GTTTCCCGCC TGAACGGCTC CTCCCTGCAC 
CTGGAATGGA GTGCCCCCCT GGAGTCTGGT GGCCGAGAGG ACCTCACCTA CGCCCTCCGC 
TGCCGGGAGT GCCGACCCGG AGGCTCCTGT GCGCCCTGCG GGGGAGACCT GACTTTTGAC 
CCCGGCCCCC GGGACCTGGT GGAGCCCTGG GTGGTGGTTC GAGGGCTACG TCCTGACTTC 
ACCTATACCT TTGAGGTCAC TGCATTGAAC GGGGTATCCT CCTTAGCCAC GGGGCCCGTC 
CCATTTGAGC CTGTCAATGT CACCACTGAC CGAGAGGTTA AGGGACAGAG CTACCATGCA 
CAAGGGTTCC CTGTGTCTCT GCCTCTCACA GTACCTCCTG CAGTGTCTGA CATCCGGGTG 
ACGCGGTCCT CACCCAGCAG CTTGAGCCTG GCCTGGGCTG TTCCCCGGGC ACCCAGTGGG 
GCTGTGCTGG ACTACGAGGT CAAATACCAT GAGAAGGGCG CCGAGGGTCC CAGCAGCGTG 
CGGTTCCTGA AGACGTCAGA AAACCGGGCA GAGCTGCGGG GGCTGAAGCG GGGAGCCAGC 
TACCTGGTGC AGGTACGGGC GCGCTCTGAG GCCGGCTACG GGCCCTTCGG CCAGGAACAT 
CACAGCCAGA CCCAACTGGA TGAGAGCGAG GGCTGGCGGG AGCAGCTGGC CCTGATTGCG 
GGCACGGCAG TCGTGGGTGT GGTCCTGGTC CTGGTGGTCA TTGTGGTCGC AGTTCTCTGC 
CTCAGGAAGC AGAGCAATGG GAGAGAAGCA GAATATTCGG ACAAACACGG ACAGTATCTC 
ATCGGACATG GTACTAAGGT CTACATCGAC CCCTTCACTT ATGAAGACCC TAATGAGGCT 
GTGAGGGAAT TTGCAAAAGA GATCGATGTC TCCTACGTCA AGATTGAAGA GGTGATTGGT 
GCAGGTGAGT TTGGCGAGGT GTGCCGGGGG CGGCTCAAGG CCCCAGGGAA GAAGGAGAGC 
TGTGTGGCAA TCAAGACCCT GAAGGGTGGC TACACGGAGC GGCAGCGGCG TGAGTTTCTG 
AGCGAGGCCT CCATCATGGG CCAGTTCGAG CACCCCAATA TCATCCGCCT GGAGGGCGTG 
GTCACCAACA GCATGCCCGT CATGATTCTC ACAGAGTTCA TGGAGAACGG CGCCCTGGAC 
TCCTTCCTGC GGCTAAACGA CGGACAGTTC ACAGTCATCC AGCTCGTGGG CATGCTGCGG 
GGCATCGCCT CGGGCATGCG GTACCTTGCC GAGATGAGCT ACGTCCACCG AGACCTGGCT 
GCTCGCAACA TCCTAGTCAA CAGCAACCTC GTCTGCAAAG TGTCTGACTT TGGCCTTTCC 
CGATTCCTGG AGGAGAACTC TTCCGATCCC ACCTACACGA GCTCCCTGGG AGGAAAGATT 
CCCATCCGAT GGACTGCCCC GGAGGCCATT GCCTTCCGGA AGTTCACTTC CGCCAGTGAT 
GCCTGGAGTT ACGGGATTGT GATGTGGGAG GTGATGTCAT TTGGGGAGAG GCCGTACTGG 
GACATGAGCA ATCAGGACGT GATCAATGCC ATTGAACAGG ACTACCGGCT GCCCCCGCCC 
CCAGACTGTC CCACCTCCCT CCACCAGCTC ATGCTGGACT GTTGGCAGAA AGACCGGAAT 
GCCCGGCCCC GCTTCCCCCA GGTGGTCAGC GCCCTGGACA AGATGATCCG GAACCCCGCC 
AGCCTCAAAA TCGTGGCCCG GGAGAATGGC GGGGCCTCAC ACCCTCTCCT GGACCAGCGG 
CAGCCTCACT ACTCAGCTTT TGGCTCTGTG GGCGAGTGGC TTCGGGCCAT CAAAATGGGA 
AGATACGAAG AAAGTTTCGC AGCCGCTGGC TTTGGCTCCT TCGAGCTGGT CAGCCAGATC 
TCTGCTGAGG ACCTGCTCCG AATCGGAGTC ACTCTGGCGG GACACCAGAA GAAAATCTTG 
GCCAGTGTCC AGCACATGAA GTCCCAGGCC AAGCCGGGAA CCCCGGGTGG GACAGGAGGA 
CCGGCCCCGC AGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EPHB4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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