NEK10 cDNA ORF clone, Homo sapiens(human)

The following NEK10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NEK10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu31682 NM_199347.4
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$354.50-$496.30
$709.00
OHu76710 NM_001031741.3
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $349.30
$499.00
OHu76711 NM_001304384.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu76709 XM_011533415.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu36997 XM_017005774.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36997 XM_011533413.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36998 XM_006713002.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu76708 XM_011533414.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu105312 XM_024453374.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu93839 XM_017005773.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu93835 XM_017005767.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93835 XM_024453373.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93836 XM_017005768.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93837 XM_017005769.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93837 XM_017005770.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93838 XM_017005772.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu36991 NM_152534.4
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005765.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005764.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005763.1
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005762.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005761.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36992 XM_006712997.3
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu36992 XM_017005766.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu36993 XM_006712998.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu36994 XM_006712999.3
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu36996 XM_006713001.3
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu36996 XM_017005771.2
Latest version!
Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
View Old Accession Versions >>
OHu36992 XM_017005766.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu31682 NM_199347.3 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$354.50-$496.30
$709.00
OHu36996 XM_017005771.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu93838 XM_017005772.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu36997 XM_017005774.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu36991 XM_017005762.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu36991 XM_017005761.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu93835 XM_017005767.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu93837 XM_017005770.1 Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu31682
    Clone ID Related Accession (Same CDS sequence) NM_199347.4 , NM_199347.3
    Accession Version NM_199347.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK123061.1, BX537852.1 and AK098832.1. On Jun 2, 2019 this sequence version replaced NM_199347.3. Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, compared to variant 2, resulting in a different 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 2. ##Evidence-Data-START## Transcript exon combination :: AK123061.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC
    CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955379.2
    CDS237..2375
    Translation

    Target ORF information:

    RefSeq Version NM_199347.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199347.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC
    CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu31682
    Clone ID Related Accession (Same CDS sequence) NM_199347.4 , NM_199347.3
    Accession Version NM_199347.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB094082.1, AK123061.1, BX537852.1 and AK098832.1. On Jan 22, 2015 this sequence version replaced NM_199347.2. Transcript Variant: This variant (3) lacks multiple 3' coding exons and contains an alternate 3' terminal exon, compared to variant 2, resulting in a different 3' coding region and 3' UTR. The encoded isoform (3) has a distinct C-terminus and is shorter than isoform 2. ##Evidence-Data-START## Transcript exon combination :: AK123061.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC
    CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955379.2
    CDS314..2452
    Translation

    Target ORF information:

    RefSeq Version NM_199347.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199347.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGTGCAGCAC
    CTCTACCTTC GCTCTCCTGC TCCTGCTCTG GCCACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76710
    Clone ID Related Accession (Same CDS sequence) NM_001031741.3
    Accession Version NM_001031741.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1425bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB045668.1, BC045758.1 and AK098832.1. On Jan 22, 2015 this sequence version replaced NM_001031741.2. Transcript Variant: This variant (1) differs in the 5' UTR and contains multiple differences in the coding region compared to variant 2, including lacking multiple 5' coding exons. It initiates translation at an alternate start codon. The encoded isoform (1) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC045758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
    AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC
    TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA
    GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT
    GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG
    AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA
    CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT
    GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT
    GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC
    CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC
    TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT
    TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA
    AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA
    GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA
    CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG
    TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGCATC AGCAGGAATT
    GCTGTGTCCC AGAGGAAAGT GCGTCAGATC AGTGATCCTA TTCAGCAGAT ATTAATTCAG
    CTGCACAAAA TAATCTATAT CACACAGCTT CCTCCAGCTT TGCACCACAA TTTGAAAAGA
    AGGGTTATAG AGAGATTCAA GAAATCCCTC TTCAGCCAGC AGAGTAACCC TTGTAATTTG
    AAATCTGAAA TTAAAAAGTT ATCTCAGGGA TCTCCAGAAC CGATTGAGCC CAACTTTTTC
    ACAGCAGATT ACCATTTATT ACATCGTTCA TCCGGTGGAA ACAGCCTGTC CCCAAATGAC
    CCTACAGGTT TACCAACCAG CATTGAATTG GAGGAAGGAA TAACATATGA ACAGATGCAG
    ACTGTGATTG AAGAAGTCCT TGAGGAAAGT GGCTATTACA ATTTTACATC TAACAGGTAT
    CATTCCTATC CATGGGGGAC CAAGAATCAC CCAACCAAAA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001026911.1
    CDS278..1702
    Translation

    Target ORF information:

    RefSeq Version NM_001031741.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001031741.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
    AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC
    TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA
    GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT
    GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG
    AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA
    CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT
    GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT
    GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC
    CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC
    TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT
    TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA
    AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA
    GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA
    CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG
    TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGCATC AGCAGGAATT
    GCTGTGTCCC AGAGGAAAGT GCGTCAGATC AGTGATCCTA TTCAGCAGAT ATTAATTCAG
    CTGCACAAAA TAATCTATAT CACACAGCTT CCTCCAGCTT TGCACCACAA TTTGAAAAGA
    AGGGTTATAG AGAGATTCAA GAAATCCCTC TTCAGCCAGC AGAGTAACCC TTGTAATTTG
    AAATCTGAAA TTAAAAAGTT ATCTCAGGGA TCTCCAGAAC CGATTGAGCC CAACTTTTTC
    ACAGCAGATT ACCATTTATT ACATCGTTCA TCCGGTGGAA ACAGCCTGTC CCCAAATGAC
    CCTACAGGTT TACCAACCAG CATTGAATTG GAGGAAGGAA TAACATATGA ACAGATGCAG
    ACTGTGATTG AAGAAGTCCT TGAGGAAAGT GGCTATTACA ATTTTACATC TAACAGGTAT
    CATTCCTATC CATGGGGGAC CAAGAATCAC CCAACCAAAA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76711
    Clone ID Related Accession (Same CDS sequence) NM_001304384.1
    Accession Version NM_001304384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1455bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform 4
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB045668.1, AK057247.1, AC093555.2, AC098931.3 and BC045758.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks multiple 5' coding exons, compared to variant 2. It initiates translation at an alternate start codon. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK057247.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
    AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC
    TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA
    GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT
    GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG
    AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA
    CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT
    GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT
    GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC
    CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC
    TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT
    TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA
    AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA
    GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA
    CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG
    TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGGGCC ACAGATGGGC
    ACATTCTTGT GGCAAGCATC AGCAGGAATT GCTGTGTCCC AGAGGAAAGT GCGTCAGATC
    AGTGATCCTA TTCAGCAGAT ATTAATTCAG CTGCACAAAA TAATCTATAT CACACAGCTT
    CCTCCAGCTT TGCACCACAA TTTGAAAAGA AGGGTTATAG AGAGATTCAA GAAATCCCTC
    TTCAGCCAGC AGAGTAACCC TTGTAATTTG AAATCTGAAA TTAAAAAGTT ATCTCAGGGA
    TCTCCAGAAC CGATTGAGCC CAACTTTTTC ACAGCAGATT ACCATTTATT ACATCGTTCA
    TCCGGTGGAA ACAGCCTGTC CCCAAATGAC CCTACAGGTT TACCAACCAG CATTGAATTG
    GAGGAAGGAA TAACATATGA ACAGATGCAG ACTGTGATTG AAGAAGTCCT TGAGGAAAGT
    GGCTATTACA ATTTTACATC TAACAGGTAT CATTCCTATC CATGGGGGAC CAAGAATCAC
    CCAACCAAAA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291313.1
    CDS278..1732
    Translation

    Target ORF information:

    RefSeq Version NM_001304384.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGTTCCTG TACAGCCTGC AGAACCCCCC GAGGTACTGA AGAGTGAGCC GTATGGGGAG 
    AAGGCTGATG TCTGGGCAGT AGGCTGCATC CTTTATCAGA TGGCGACTTT GAGTCCCCCC
    TTCTACAGCA CTAACATGCT GTCCTTGGCT ACAAAAATAG TGGAGGCGGT ATATGAACCA
    GTCCCAGAAG GTATCTACTC TGAAAAAGTA ACAGACACCA TCAGCAGGTG CCTCACTCCT
    GATGCGGAAG CTCGTCCAGA TATTGTAGAA GTCAGTTCGA TGATATCAGA TGTCATGATG
    AAATATTTAG ACAACTTATC TACATCCCAG TTGTCCTTGG AAAAGAAGCT AGAACGGGAA
    CGAAGACGCA CACAAAGGTA TTTTATGGAA GCCAACCGGA ACACCGTCAC ATGTCACCAT
    GAGCTGGCTG TTCTATCTCA CGAGACCTTT GAGAAGGCAA GTTTGAGTAG CAGCAGCAGT
    GGAGCAGCCA GCCTGAAAAG TGAACTTTCA GAAAGCGCAG ACCTGCCCCC TGAAGGCTTC
    CAGGCCTCCT ATGGTAAAGA CGAAGACAGG GCCTGTGACG AAATCCTGTC AGATGATAAC
    TTCAACCTGG AAAATGCTGA GAAAGATACA TATTCAGAGG TAGATGATGA ATTGGACATT
    TCGGATAACT CCAGCAGCTC CAGTTCAAGC CCTCTGAAAG AATCTACATT CAACATTTTA
    AAGAGAAGTT TTAGTGCTTC AGGAGGAGAA AGACAATCCC AAACAAGGGA CTTCACTGGA
    GGAACAGGAT CAAGACCAAG ACCAGCTTTG CTGCCTCTTG ACCTGCTTCT GAAAGTGCCA
    CCCCACATGC TCAGGGCCCA CATTAAGGAA ATAGAGGCTG AGTTAGTGAC AGGGTGGCAG
    TCCCATAGCC TTCCTGCTGT GATTCTTCGA AATCTCAAAG ATCATGGGCC ACAGATGGGC
    ACATTCTTGT GGCAAGCATC AGCAGGAATT GCTGTGTCCC AGAGGAAAGT GCGTCAGATC
    AGTGATCCTA TTCAGCAGAT ATTAATTCAG CTGCACAAAA TAATCTATAT CACACAGCTT
    CCTCCAGCTT TGCACCACAA TTTGAAAAGA AGGGTTATAG AGAGATTCAA GAAATCCCTC
    TTCAGCCAGC AGAGTAACCC TTGTAATTTG AAATCTGAAA TTAAAAAGTT ATCTCAGGGA
    TCTCCAGAAC CGATTGAGCC CAACTTTTTC ACAGCAGATT ACCATTTATT ACATCGTTCA
    TCCGGTGGAA ACAGCCTGTC CCCAAATGAC CCTACAGGTT TACCAACCAG CATTGAATTG
    GAGGAAGGAA TAACATATGA ACAGATGCAG ACTGTGATTG AAGAAGTCCT TGAGGAAAGT
    GGCTATTACA ATTTTACATC TAACAGGTAT CATTCCTATC CATGGGGGAC CAAGAATCAC
    CCAACCAAAA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76709
    Clone ID Related Accession (Same CDS sequence) XM_011533415.1
    Accession Version XM_011533415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC
    ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG
    ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT
    ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC
    AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG
    GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC
    ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGTGGTTTCA TGAGAAGATG CAAAGAAACT
    GTGGCAGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531717.1
    CDS275..2266
    Translation

    Target ORF information:

    RefSeq Version XM_011533415.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC
    ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG
    ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT
    ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC
    AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG
    GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC
    ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGTGGTTTCA TGAGAAGATG CAAAGAAACT
    GTGGCAGGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36997
    Clone ID Related Accession (Same CDS sequence) XM_017005774.2 , XM_017005774.1 , XM_011533413.2
    Accession Version XM_017005774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861263.1
    CDS274..2415
    Translation

    Target ORF information:

    RefSeq Version XM_017005774.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36997
    Clone ID Related Accession (Same CDS sequence) XM_017005774.2 , XM_017005774.1 , XM_011533413.2
    Accession Version XM_017005774.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861263.1
    CDS227..2368
    Translation

    Target ORF information:

    RefSeq Version XM_017005774.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36997
    Clone ID Related Accession (Same CDS sequence) XM_017005774.2 , XM_017005774.1 , XM_011533413.2
    Accession Version XM_011533413.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531715.1
    CDS275..2416
    Translation

    Target ORF information:

    RefSeq Version XM_011533413.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533413.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCTTTG AAACCATCCA AGGAAACAGA
    CCCCGATTCA GTGGTACATG GGGTGAGACA CAGTCAACAT TTGCCCTAAA TACTGTCACT
    TGCCAAATAA GGTCCATATT GTGCCATCAC ATTACAAAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36998
    Clone ID Related Accession (Same CDS sequence) XM_006713002.1
    Accession Version XM_006713002.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGGTTTCATG
    AGAAGATGCA AAGAAACTGT GGCAGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713065.1
    CDS275..2404
    Translation

    Target ORF information:

    RefSeq Version XM_006713002.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006713002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG TGGTTTCATG
    AGAAGATGCA AAGAAACTGT GGCAGGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu76708
    Clone ID Related Accession (Same CDS sequence) XM_011533414.2
    Accession Version XM_011533414.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2028bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533414.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCCCGA GGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531716.1
    CDS275..2302
    Translation

    Target ORF information:

    RefSeq Version XM_011533414.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533414.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACCCCGA GGTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu105312
    Clone ID Related Accession (Same CDS sequence) XM_024453374.1
    Accession Version XM_024453374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2139bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGACCCCTT TATTCTTTCA GGAAAATGGT GTATATACAA TAGCAAAATT AATTTTACCA 
    AATAAGCAAA AGAATGCAGC AAAAAGTAAT CTATTACAGT GTTATGCTTT CAGAGCCTTG
    AGATTTCTCT TCAGTATGGA AAGAAACAGA CCACTCTTTA AAAGACTTTT CCCCACAGAC
    TTGTTTGAGA TCTTCATTGA CATAGGGCAT TATGTACGTG ATATCAGTGC TTATGAAGAA
    TTGGTATCCA AGCTGAATTT ATTAGTGGAG GATGAACTGA AGCAAATTGC TGAAAATATT
    GAAAGCATTA ATCAGAACAA AGCTCCTTTG AAATATATAG GCAACTATGC AATTTTGGAT
    CATCTTGGAA GTGGAGCTTT TGGCTGTGTT TACAAGGTTA GAAAGCATAG TGGTCAAAAT
    CTTTTAGCAA TGAAAGAGGT CAATTTACAT AACCCAGCAT TTGGAAAGGA TAAGAAAGAT
    CGAGACAGCA GCGTAAGGAA TATTGTTTCT GAATTAACAA TAATTAAAGA GCAGCTTTAT
    CATCCCAACA TTGTACGTTA TTACAAAACA TTTCTGGAAA ATGATAGGTT GTACATAGTT
    ATGGAGCTGA TAGAAGGAGC CCCGCTTGGA GAGCATTTCA GTTCTTTGAA GGAAAAACAT
    CACCATTTTA CTGAAGAAAG ACTATGGAAA ATATTTATAC AGCTGTGCTT AGCTCTTCGA
    TACTTACACA AGGAGAAGAG GATTGTCCAT AGAGATCTGA CACCAAACAA CATTATGTTG
    GGGGATAAGG ACAAAGTAAC CGTTACTGAC TTTGGCCTGG CAAAGCAAAA ACAAGAAAAC
    AGTAAACTCA CCTCTGTGGT TGGAACAATC CTGTATTCTT GCCCCGAGGT ACTGAAGAGT
    GAGCCGTATG GGGAGAAGGC TGATGTCTGG GCAGTAGGCT GCATCCTTTA TCAGATGGCG
    ACTTTGAGTC CCCCCTTCTA CAGCACTAAC ATGCTGTCCT TGGCTACAAA AATAGTGGAG
    GCGGTATATG AACCAGTCCC AGAAGGTATC TACTCTGAAA AAGTAACAGA CACCATCAGC
    AGGTGCCTCA CTCCTGATGC GGAAGCTCGT CCAGATATTG TAGAAGTCAG TTCGATGATA
    TCAGATGTCA TGATGAAATA TTTAGACAAC TTATCTACAT CCCAGTTGTC CTTGGAAAAG
    AAGCTAGAAC GGGAACGAAG ACGCACACAA AGGTATTTTA TGGAAGCCAA CCGGAACACC
    GTCACATGTC ACCATGAGCT GGCTGTTCTA TCTCACGAGA CCTTTGAGAA GGCAAGTTTG
    AGTAGCAGCA GCAGTGGAGC AGCCAGCCTG AAAAGTGAAC TTTCAGAAAG CGCAGACCTG
    CCCCCTGAAG GCTTCCAGGC CTCCTATGGT AAAGACGAAG ACAGGGCCTG TGACGAAATC
    CTGTCAGATG ATAACTTCAA CCTGGAAAAT GCTGAGAAAG ATACATATTC AGAGGTAGAT
    GATGAATTGG ACATTTCGGA TAACTCCAGC AGCTCCAGTT CAAGCCCTCT GAAAGAATCT
    ACATTCAACA TTTTAAAGAG AAGTTTTAGT GCTTCAGGAG GAGAAAGACA ATCCCAAACA
    AGGGACTTCA CTGGAGGAAC AGGATCAAGA CCAAGACCAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309142.1
    CDS2782..4920
    Translation

    Target ORF information:

    RefSeq Version XM_024453374.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGACCCCTT TATTCTTTCA GGAAAATGGT GTATATACAA TAGCAAAATT AATTTTACCA 
    AATAAGCAAA AGAATGCAGC AAAAAGTAAT CTATTACAGT GTTATGCTTT CAGAGCCTTG
    AGATTTCTCT TCAGTATGGA AAGAAACAGA CCACTCTTTA AAAGACTTTT CCCCACAGAC
    TTGTTTGAGA TCTTCATTGA CATAGGGCAT TATGTACGTG ATATCAGTGC TTATGAAGAA
    TTGGTATCCA AGCTGAATTT ATTAGTGGAG GATGAACTGA AGCAAATTGC TGAAAATATT
    GAAAGCATTA ATCAGAACAA AGCTCCTTTG AAATATATAG GCAACTATGC AATTTTGGAT
    CATCTTGGAA GTGGAGCTTT TGGCTGTGTT TACAAGGTTA GAAAGCATAG TGGTCAAAAT
    CTTTTAGCAA TGAAAGAGGT CAATTTACAT AACCCAGCAT TTGGAAAGGA TAAGAAAGAT
    CGAGACAGCA GCGTAAGGAA TATTGTTTCT GAATTAACAA TAATTAAAGA GCAGCTTTAT
    CATCCCAACA TTGTACGTTA TTACAAAACA TTTCTGGAAA ATGATAGGTT GTACATAGTT
    ATGGAGCTGA TAGAAGGAGC CCCGCTTGGA GAGCATTTCA GTTCTTTGAA GGAAAAACAT
    CACCATTTTA CTGAAGAAAG ACTATGGAAA ATATTTATAC AGCTGTGCTT AGCTCTTCGA
    TACTTACACA AGGAGAAGAG GATTGTCCAT AGAGATCTGA CACCAAACAA CATTATGTTG
    GGGGATAAGG ACAAAGTAAC CGTTACTGAC TTTGGCCTGG CAAAGCAAAA ACAAGAAAAC
    AGTAAACTCA CCTCTGTGGT TGGAACAATC CTGTATTCTT GCCCCGAGGT ACTGAAGAGT
    GAGCCGTATG GGGAGAAGGC TGATGTCTGG GCAGTAGGCT GCATCCTTTA TCAGATGGCG
    ACTTTGAGTC CCCCCTTCTA CAGCACTAAC ATGCTGTCCT TGGCTACAAA AATAGTGGAG
    GCGGTATATG AACCAGTCCC AGAAGGTATC TACTCTGAAA AAGTAACAGA CACCATCAGC
    AGGTGCCTCA CTCCTGATGC GGAAGCTCGT CCAGATATTG TAGAAGTCAG TTCGATGATA
    TCAGATGTCA TGATGAAATA TTTAGACAAC TTATCTACAT CCCAGTTGTC CTTGGAAAAG
    AAGCTAGAAC GGGAACGAAG ACGCACACAA AGGTATTTTA TGGAAGCCAA CCGGAACACC
    GTCACATGTC ACCATGAGCT GGCTGTTCTA TCTCACGAGA CCTTTGAGAA GGCAAGTTTG
    AGTAGCAGCA GCAGTGGAGC AGCCAGCCTG AAAAGTGAAC TTTCAGAAAG CGCAGACCTG
    CCCCCTGAAG GCTTCCAGGC CTCCTATGGT AAAGACGAAG ACAGGGCCTG TGACGAAATC
    CTGTCAGATG ATAACTTCAA CCTGGAAAAT GCTGAGAAAG ATACATATTC AGAGGTAGAT
    GATGAATTGG ACATTTCGGA TAACTCCAGC AGCTCCAGTT CAAGCCCTCT GAAAGAATCT
    ACATTCAACA TTTTAAAGAG AAGTTTTAGT GCTTCAGGAG GAGAAAGACA ATCCCAAACA
    AGGGACTTCA CTGGAGGAAC AGGATCAAGA CCAAGACCAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93839
    Clone ID Related Accession (Same CDS sequence) XM_017005773.1
    Accession Version XM_017005773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2838bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861262.1
    CDS275..3112
    Translation

    Target ORF information:

    RefSeq Version XM_017005773.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93835
    Clone ID Related Accession (Same CDS sequence) XM_017005767.1 , XM_024453373.1 , XM_017005767.2
    Accession Version XM_017005767.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861256.1
    CDS227..3658
    Translation

    Target ORF information:

    RefSeq Version XM_017005767.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005767.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93835
    Clone ID Related Accession (Same CDS sequence) XM_017005767.1 , XM_024453373.1 , XM_017005767.2
    Accession Version XM_024453373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024309141.1
    CDS275..3706
    Translation

    Target ORF information:

    RefSeq Version XM_024453373.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93835
    Clone ID Related Accession (Same CDS sequence) XM_017005767.1 , XM_024453373.1 , XM_017005767.2
    Accession Version XM_017005767.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3432bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005767.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861256.1
    CDS274..3705
    Translation

    Target ORF information:

    RefSeq Version XM_017005767.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005767.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGCTTTGCTG CCTCTTGACC TGCTTCTGAA AGTGCCACCC
    CACATGCTCA GGGCCCACAT TAAGGAAATA GAGGCTGAGT TAGTGACAGG GTGGCAGTCC
    CATAGCCTTC CTGCTGTGAT TCTTCGAAAT CTCAAAGATC ATGGGCCACA GATGGGCACA
    TTCTTGTGGC AAGCATCAGC AGGAATTGCT GTGTCCCAGA GGAAAGTGCG TCAGATCAGT
    GATCCTATTC AGCAGATATT AATTCAGCTG CACAAAATAA TCTATATCAC ACAGCTTCCT
    CCAGCTTTGC ACCACAATTT GAAAAGAAGG GTTATAGAGA GATTCAAGAA ATCCCTCTTC
    AGCCAGCAGA GTAACCCTTG TAATTTGAAA TCTGAAATTA AAAAGTTATC TCAGGGATCT
    CCAGAACCGA TTGAGCCCAA CTTTTTCACA GCAGATTACC ATTTATTACA TCGTTCATCC
    GGTGGAAACA GCCTGTCCCC AAATGACCCT ACAGGTTTAC CAACCAGCAT TGAATTGGAG
    GAAGGAATAA CATATGAACA GATGCAGACT GTGATTGAAG AAGTCCTTGA GGAAAGTGGC
    TATTACAATT TTACATCTAA CAGGTATCAT TCCTATCCAT GGGGGACCAA GAATCACCCA
    ACCAAAAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93836
    Clone ID Related Accession (Same CDS sequence) XM_017005768.1
    Accession Version XM_017005768.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3381bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC
    ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG
    ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT
    ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC
    AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG
    GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC
    ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGCCCCGAGG TACTGAAGAG TGAGCCGTAT
    GGGGAGAAGG CTGATGTCTG GGCAGTAGGC TGCATCCTTT ATCAGATGGC GACTTTGAGT
    CCCCCCTTCT ACAGCACTAA CATGCTGTCC TTGGCTACAA AAATAGTGGA GGCGGTATAT
    GAACCAGTCC CAGAAGGTAT CTACTCTGAA AAAGTAACAG ACACCATCAG CAGGTGCCTC
    ACTCCTGATG CGGAAGCTCG TCCAGATATT GTAGAAGTCA GTTCGATGAT ATCAGATGTC
    ATGATGAAAT ATTTAGACAA CTTATCTACA TCCCAGTTGT CCTTGGAAAA GAAGCTAGAA
    CGGGAACGAA GACGCACACA AAGGTATTTT ATGGAAGCCA ACCGGAACAC CGTCACATGT
    CACCATGAGC TGGCTGTTCT ATCTCACGAG ACCTTTGAGA AGGCAAGTTT GAGTAGCAGC
    AGCAGTGGAG CAGCCAGCCT GAAAAGTGAA CTTTCAGAAA GCGCAGACCT GCCCCCTGAA
    GGCTTCCAGG CCTCCTATGG TAAAGACGAA GACAGGGCCT GTGACGAAAT CCTGTCAGAT
    GATAACTTCA ACCTGGAAAA TGCTGAGAAA GATACATATT CAGAGGTAGA TGATGAATTG
    GACATTTCGG ATAACTCCAG CAGCTCCAGT TCAAGCCCTC TGAAAGAATC TACATTCAAC
    ATTTTAAAGA GAAGTTTTAG TGCTTCAGGA GGAGAAAGAC AATCCCAAAC AAGGGACTTC
    ACTGGAGGAA CAGGATCAAG ACCAAGACCA GCTTTGCTGC CTCTTGACCT GCTTCTGAAA
    GTGCCACCCC ACATGCTCAG GGCCCACATT AAGGAAATAG AGGCTGAGTT AGTGACAGGG
    TGGCAGTCCC ATAGCCTTCC TGCTGTGATT CTTCGAAATC TCAAAGATCA TGGGCCACAG
    ATGGGCACAT TCTTGTGGCA AGCATCAGCA GGAATTGCTG TGTCCCAGAG GAAAGTGCGT
    CAGATCAGTG ATCCTATTCA GCAGATATTA ATTCAGCTGC ACAAAATAAT CTATATCACA
    CAGCTTCCTC CAGCTTTGCA CCACAATTTG AAAAGAAGGG TTATAGAGAG ATTCAAGAAA
    TCCCTCTTCA GCCAGCAGAG TAACCCTTGT AATTTGAAAT CTGAAATTAA AAAGTTATCT
    CAGGGATCTC CAGAACCGAT TGAGCCCAAC TTTTTCACAG CAGATTACCA TTTATTACAT
    CGTTCATCCG GTGGAAACAG CCTGTCCCCA AATGACCCTA CAGGTTTACC AACCAGCATT
    GAATTGGAGG AAGGAATAAC ATATGAACAG ATGCAGACTG TGATTGAAGA AGTCCTTGAG
    GAAAGTGGCT ATTACAATTT TACATCTAAC AGGTATCATT CCTATCCATG GGGGACCAAG
    AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861257.1
    CDS275..3655
    Translation

    Target ORF information:

    RefSeq Version XM_017005768.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005768.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGCTTTA TCATCCCAAC
    ATTGTACGTT ATTACAAAAC ATTTCTGGAA AATGATAGGT TGTACATAGT TATGGAGCTG
    ATAGAAGGAG CCCCGCTTGG AGAGCATTTC AGTTCTTTGA AGGAAAAACA TCACCATTTT
    ACTGAAGAAA GACTATGGAA AATATTTATA CAGCTGTGCT TAGCTCTTCG ATACTTACAC
    AAGGAGAAGA GGATTGTCCA TAGAGATCTG ACACCAAACA ACATTATGTT GGGGGATAAG
    GACAAAGTAA CCGTTACTGA CTTTGGCCTG GCAAAGCAAA AACAAGAAAA CAGTAAACTC
    ACCTCTGTGG TTGGAACAAT CCTGTATTCT TGCCCCGAGG TACTGAAGAG TGAGCCGTAT
    GGGGAGAAGG CTGATGTCTG GGCAGTAGGC TGCATCCTTT ATCAGATGGC GACTTTGAGT
    CCCCCCTTCT ACAGCACTAA CATGCTGTCC TTGGCTACAA AAATAGTGGA GGCGGTATAT
    GAACCAGTCC CAGAAGGTAT CTACTCTGAA AAAGTAACAG ACACCATCAG CAGGTGCCTC
    ACTCCTGATG CGGAAGCTCG TCCAGATATT GTAGAAGTCA GTTCGATGAT ATCAGATGTC
    ATGATGAAAT ATTTAGACAA CTTATCTACA TCCCAGTTGT CCTTGGAAAA GAAGCTAGAA
    CGGGAACGAA GACGCACACA AAGGTATTTT ATGGAAGCCA ACCGGAACAC CGTCACATGT
    CACCATGAGC TGGCTGTTCT ATCTCACGAG ACCTTTGAGA AGGCAAGTTT GAGTAGCAGC
    AGCAGTGGAG CAGCCAGCCT GAAAAGTGAA CTTTCAGAAA GCGCAGACCT GCCCCCTGAA
    GGCTTCCAGG CCTCCTATGG TAAAGACGAA GACAGGGCCT GTGACGAAAT CCTGTCAGAT
    GATAACTTCA ACCTGGAAAA TGCTGAGAAA GATACATATT CAGAGGTAGA TGATGAATTG
    GACATTTCGG ATAACTCCAG CAGCTCCAGT TCAAGCCCTC TGAAAGAATC TACATTCAAC
    ATTTTAAAGA GAAGTTTTAG TGCTTCAGGA GGAGAAAGAC AATCCCAAAC AAGGGACTTC
    ACTGGAGGAA CAGGATCAAG ACCAAGACCA GCTTTGCTGC CTCTTGACCT GCTTCTGAAA
    GTGCCACCCC ACATGCTCAG GGCCCACATT AAGGAAATAG AGGCTGAGTT AGTGACAGGG
    TGGCAGTCCC ATAGCCTTCC TGCTGTGATT CTTCGAAATC TCAAAGATCA TGGGCCACAG
    ATGGGCACAT TCTTGTGGCA AGCATCAGCA GGAATTGCTG TGTCCCAGAG GAAAGTGCGT
    CAGATCAGTG ATCCTATTCA GCAGATATTA ATTCAGCTGC ACAAAATAAT CTATATCACA
    CAGCTTCCTC CAGCTTTGCA CCACAATTTG AAAAGAAGGG TTATAGAGAG ATTCAAGAAA
    TCCCTCTTCA GCCAGCAGAG TAACCCTTGT AATTTGAAAT CTGAAATTAA AAAGTTATCT
    CAGGGATCTC CAGAACCGAT TGAGCCCAAC TTTTTCACAG CAGATTACCA TTTATTACAT
    CGTTCATCCG GTGGAAACAG CCTGTCCCCA AATGACCCTA CAGGTTTACC AACCAGCATT
    GAATTGGAGG AAGGAATAAC ATATGAACAG ATGCAGACTG TGATTGAAGA AGTCCTTGAG
    GAAAGTGGCT ATTACAATTT TACATCTAAC AGGTATCATT CCTATCCATG GGGGACCAAG
    AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93837
    Clone ID Related Accession (Same CDS sequence) XM_017005769.1 , XM_017005770.1 , XM_017005770.2
    Accession Version XM_017005769.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861258.1
    CDS275..3652
    Translation

    Target ORF information:

    RefSeq Version XM_017005769.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005769.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93837
    Clone ID Related Accession (Same CDS sequence) XM_017005769.1 , XM_017005770.1 , XM_017005770.2
    Accession Version XM_017005770.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861259.1
    CDS227..3604
    Translation

    Target ORF information:

    RefSeq Version XM_017005770.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93837
    Clone ID Related Accession (Same CDS sequence) XM_017005769.1 , XM_017005770.1 , XM_017005770.2
    Accession Version XM_017005770.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3378bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861259.1
    CDS274..3651
    Translation

    Target ORF information:

    RefSeq Version XM_017005770.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005770.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGG GCCACAGATG
    GGCACATTCT TGTGGCAAGC ATCAGCAGGA ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG
    ATCAGTGATC CTATTCAGCA GATATTAATT CAGCTGCACA AAATAATCTA TATCACACAG
    CTTCCTCCAG CTTTGCACCA CAATTTGAAA AGAAGGGTTA TAGAGAGATT CAAGAAATCC
    CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT TTGAAATCTG AAATTAAAAA GTTATCTCAG
    GGATCTCCAG AACCGATTGA GCCCAACTTT TTCACAGCAG ATTACCATTT ATTACATCGT
    TCATCCGGTG GAAACAGCCT GTCCCCAAAT GACCCTACAG GTTTACCAAC CAGCATTGAA
    TTGGAGGAAG GAATAACATA TGAACAGATG CAGACTGTGA TTGAAGAAGT CCTTGAGGAA
    AGTGGCTATT ACAATTTTAC ATCTAACAGG TATCATTCCT ATCCATGGGG GACCAAGAAT
    CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93838
    Clone ID Related Accession (Same CDS sequence) XM_017005772.2 , XM_017005772.1
    Accession Version XM_017005772.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA
    GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA
    ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG
    AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC
    TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA
    AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC
    AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861261.1
    CDS274..3564
    Translation

    Target ORF information:

    RefSeq Version XM_017005772.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005772.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA
    GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA
    ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG
    AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC
    TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA
    AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC
    AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93838
    Clone ID Related Accession (Same CDS sequence) XM_017005772.2 , XM_017005772.1
    Accession Version XM_017005772.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA
    GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA
    ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG
    AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC
    TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA
    AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC
    AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861261.1
    CDS227..3517
    Translation

    Target ORF information:

    RefSeq Version XM_017005772.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGACTA
    ACAGCGGAGT TGCTGCGCCT ACTTTGTGCA GAGCCCCAGG TGAAAGAGCA GGTGAAGCTC
    TATGAGGGGA TACCGGTCCT CCTCAGTCTG CTCCACTCTG ACCACTTGAA GCTCCTCTGG
    AGCATTGTCT GGATTCTGGT ACAGGTTTGT GAGGACCCTG AGACCAGCGT GGAAATTCGC
    ATTTGGGGAG GCATCAAACA GCTTCTTCAT ATTTTACAAG GAGACAGAAA TTTTGTTTCT
    GATCACTCCT CCATTGGAAG CCTGTCCAGT GCAAATGCTG CAGGCCGAAT CCAGCAGCTT
    CATTTATCAG AAGACTTGAG CCCTAGGGAA ATACAAGAAA ATACTTTCTC ACTTCAAGCA
    GCCTGCTGTG CTGCCCTCAC TGAGCTGGTG CTCAATGACA CCAATGCCCA CCAGGTGGTT
    CAGGAAAATG GTGTATATAC AATAGCAAAA TTAATTTTAC CAAATAAGCA AAAGAATGCA
    GCAAAAAGTA ATCTATTACA GTGTTATGCT TTCAGAGCCT TGAGATTTCT CTTCAGTATG
    GAAAGAAACA GACCACTCTT TAAAAGACTT TTCCCCACAG ACTTGTTTGA GATCTTCATT
    GACATAGGGC ATTATGTACG TGATATCAGT GCTTATGAAG AATTGGTATC CAAGCTGAAT
    TTATTAGTGG AGGATGAACT GAAGCAAATT GCTGAAAATA TTGAAAGCAT TAATCAGAAC
    AAAGCTCCTT TGAAATATAT AGGCAACTAT GCAATTTTGG ATCATCTTGG AAGTGGAGCT
    TTTGGCTGTG TTTACAAGGT TAGAAAGCAT AGTGGTCAAA ATCTTTTAGC AATGAAAGAG
    GTCAATTTAC ATAACCCAGC ATTTGGAAAG GATAAGAAAG ATCGAGACAG CAGCGTAAGG
    AATATTGTTT CTGAATTAAC AATAATTAAA GAGCAGCTTT ATCATCCCAA CATTGTACGT
    TATTACAAAA CATTTCTGGA AAATGATAGG TTGTACATAG TTATGGAGCT GATAGAAGGA
    GCCCCGCTTG GAGAGCATTT CAGTTCTTTG AAGGAAAAAC ATCACCATTT TACTGAAGAA
    AGACTATGGA AAATATTTAT ACAGCTGTGC TTAGCTCTTC GATACTTACA CAAGGAGAAG
    AGGATTGTCC ATAGAGATCT GACACCAAAC AACATTATGT TGGGGGATAA GGACAAAGTA
    ACCGTTACTG ACTTTGGCCT GGCAAAGCAA AAACAAGAAA ACAGTAAACT CACCTCTGTG
    GTTGGAACAA TCCTGTATTC TTGCCCCGAG GTACTGAAGA GTGAGCCGTA TGGGGAGAAG
    GCTGATGTCT GGGCAGTAGG CTGCATCCTT TATCAGATGG CGACTTTGAG TCCCCCCTTC
    TACAGCACTA ACATGCTGTC CTTGGCTACA AAAATAGTGG AGGCGGTATA TGAACCAGTC
    CCAGAAGGTA TCTACTCTGA AAAAGTAACA GACACCATCA GCAGGTGCCT CACTCCTGAT
    GCGGAAGCTC GTCCAGATAT TGTAGAAGTC AGTTCGATGA TATCAGATGT CATGATGAAA
    TATTTAGACA ACTTATCTAC ATCCCAGTTG TCCTTGGAAA AGAAGCTAGA ACGGGAACGA
    AGACGCACAC AAAGGTATTT TATGGAAGCC AACCGGAACA CCGTCACATG TCACCATGAG
    CTGGCTGTTC TATCTCACGA GACCTTTGAG AAGGCAAGTT TGAGTAGCAG CAGCAGTGGA
    GCAGCCAGCC TGAAAAGTGA ACTTTCAGAA AGCGCAGACC TGCCCCCTGA AGGCTTCCAG
    GCCTCCTATG GTAAAGACGA AGACAGGGCC TGTGACGAAA TCCTGTCAGA TGATAACTTC
    AACCTGGAAA ATGCTGAGAA AGATACATAT TCAGAGGTAG ATGATGAATT GGACATTTCG
    GATAACTCCA GCAGCTCCAG TTCAAGCCCT CTGAAAGAAT CTACATTCAA CATTTTAAAG
    AGAAGTTTTA GTGCTTCAGG AGGAGAAAGA CAATCCCAAA CAAGGGACTT CACTGGAGGA
    ACAGGATCAA GACCAAGACC AGGGCCACAG ATGGGCACAT TCTTGTGGCA AGCATCAGCA
    GGAATTGCTG TGTCCCAGAG GAAAGTGCGT CAGATCAGTG ATCCTATTCA GCAGATATTA
    ATTCAGCTGC ACAAAATAAT CTATATCACA CAGCTTCCTC CAGCTTTGCA CCACAATTTG
    AAAAGAAGGG TTATAGAGAG ATTCAAGAAA TCCCTCTTCA GCCAGCAGAG TAACCCTTGT
    AATTTGAAAT CTGAAATTAA AAAGTTATCT CAGGGATCTC CAGAACCGAT TGAGCCCAAC
    TTTTTCACAG CAGATTACCA TTTATTACAT CGTTCATCCG GTGGAAACAG CCTGTCCCCA
    AATGACCCTA CAGGTTTACC AACCAGCATT GAATTGGAGG AAGGAATAAC ATATGAACAG
    ATGCAGACTG TGATTGAAGA AGTCCTTGAG GAAAGTGGCT ATTACAATTT TACATCTAAC
    AGGTATCATT CCTATCCATG GGGGACCAAG AATCACCCAA CCAAAAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version NM_152534.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB094082.1, AK123061.1, AK098832.1, AC093555.2, BC045758.1, AC098931.3 and AK057247.1. On or before Jan 22, 2015 this sequence version replaced XM_006712994.1, NM_152534.3. Transcript Variant: This variant (2) represents the inferred longest transcript and encodes the longest isoform (2). ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_689747.3
    CDS314..3832
    Translation

    Target ORF information:

    RefSeq Version NM_152534.4
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_152534.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861253.1
    CDS204..3722
    Translation

    Target ORF information:

    RefSeq Version XM_017005764.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005762.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005762.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861251.1
    CDS387..3905
    Translation

    Target ORF information:

    RefSeq Version XM_017005762.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005762.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005761.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861250.1
    CDS227..3745
    Translation

    Target ORF information:

    RefSeq Version XM_017005761.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861252.1
    CDS372..3890
    Translation

    Target ORF information:

    RefSeq Version XM_017005763.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005762.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861251.1
    CDS391..3909
    Translation

    Target ORF information:

    RefSeq Version XM_017005762.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005762.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005761.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005761.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861250.1
    CDS274..3792
    Translation

    Target ORF information:

    RefSeq Version XM_017005761.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005761.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36991
    Clone ID Related Accession (Same CDS sequence) XM_017005764.1 , XM_017005762.2 , NM_152534.4 , XM_017005761.1 , XM_017005763.1 , XM_017005762.1 , XM_017005761.2 , XM_017005765.1
    Accession Version XM_017005765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3519bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861254.1
    CDS176..3694
    Translation

    Target ORF information:

    RefSeq Version XM_017005765.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GTATCATTCC
    TATCCATGGG GGACCAAGAA TCACCCAACC AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36992
    Clone ID Related Accession (Same CDS sequence) XM_017005766.1 , XM_017005766.2 , XM_006712997.3
    Accession Version XM_017005766.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861255.1
    CDS227..3715
    Translation

    Target ORF information:

    RefSeq Version XM_017005766.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005766.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36992
    Clone ID Related Accession (Same CDS sequence) XM_017005766.1 , XM_017005766.2 , XM_006712997.3
    Accession Version XM_017005766.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005766.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861255.1
    CDS274..3762
    Translation

    Target ORF information:

    RefSeq Version XM_017005766.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005766.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36992
    Clone ID Related Accession (Same CDS sequence) XM_017005766.1 , XM_017005766.2 , XM_006712997.3
    Accession Version XM_006712997.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713060.1
    CDS275..3763
    Translation

    Target ORF information:

    RefSeq Version XM_006712997.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712997.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG CATCAGCAGG AATTGCTGTG TCCCAGAGGA AAGTGCGTCA GATCAGTGAT
    CCTATTCAGC AGATATTAAT TCAGCTGCAC AAAATAATCT ATATCACACA GCTTCCTCCA
    GCTTTGCACC ACAATTTGAA AAGAAGGGTT ATAGAGAGAT TCAAGAAATC CCTCTTCAGC
    CAGCAGAGTA ACCCTTGTAA TTTGAAATCT GAAATTAAAA AGTTATCTCA GGGATCTCCA
    GAACCGATTG AGCCCAACTT TTTCACAGCA GATTACCATT TATTACATCG TTCATCCGGT
    GGAAACAGCC TGTCCCCAAA TGACCCTACA GGTTTACCAA CCAGCATTGA ATTGGAGGAA
    GGAATAACAT ATGAACAGAT GCAGACTGTG ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT
    TACAATTTTA CATCTAACAG GTATCATTCC TATCCATGGG GGACCAAGAA TCACCCAACC
    AAAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36993
    Clone ID Related Accession (Same CDS sequence) XM_006712998.2
    Accession Version XM_006712998.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3489bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006712998.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GGCTGAGTAT
    ATGCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713061.1
    CDS275..3763
    Translation

    Target ORF information:

    RefSeq Version XM_006712998.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712998.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG GGCTGAGTAT
    ATGCATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36994
    Clone ID Related Accession (Same CDS sequence) XM_006712999.3
    Accession Version XM_006712999.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3477bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712999.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713062.1
    CDS275..3751
    Translation

    Target ORF information:

    RefSeq Version XM_006712999.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712999.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC TTTGCTGCCT
    CTTGACCTGC TTCTGAAAGT GCCACCCCAC ATGCTCAGGG CCCACATTAA GGAAATAGAG
    GCTGAGTTAG TGACAGGGTG GCAGTCCCAT AGCCTTCCTG CTGTGATTCT TCGAAATCTC
    AAAGATCATG GGCCACAGAT GGGCACATTC TTGTGGCAAG CATCAGCAGG AATTGCTGTG
    TCCCAGAGGA AAGTGCGTCA GATCAGTGAT CCTATTCAGC AGATATTAAT TCAGCTGCAC
    AAAATAATCT ATATCACACA GCTTCCTCCA GCTTTGCACC ACAATTTGAA AAGAAGGGTT
    ATAGAGAGAT TCAAGAAATC CCTCTTCAGC CAGCAGAGTA ACCCTTGTAA TTTGAAATCT
    GAAATTAAAA AGTTATCTCA GGGATCTCCA GAACCGATTG AGCCCAACTT TTTCACAGCA
    GATTACCATT TATTACATCG TTCATCCGGT GGAAACAGCC TGTCCCCAAA TGACCCTACA
    GGTTTACCAA CCAGCATTGA ATTGGAGGAA GGAATAACAT ATGAACAGAT GCAGACTGTG
    ATTGAAGAAG TCCTTGAGGA AAGTGGCTAT TACAATTTTA CATCTAACAG TGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36996
    Clone ID Related Accession (Same CDS sequence) XM_006713001.3 , XM_017005771.1 , XM_017005771.2
    Accession Version XM_006713001.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006713064.1
    CDS275..3622
    Translation

    Target ORF information:

    RefSeq Version XM_006713001.3
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006713001.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36996
    Clone ID Related Accession (Same CDS sequence) XM_006713001.3 , XM_017005771.1 , XM_017005771.2
    Accession Version XM_017005771.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861260.1
    CDS227..3574
    Translation

    Target ORF information:

    RefSeq Version XM_017005771.1
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36996
    Clone ID Related Accession (Same CDS sequence) XM_006713001.3 , XM_017005771.1 , XM_017005771.2
    Accession Version XM_017005771.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product serine/threonine-protein kinase Nek10 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016861260.1
    CDS274..3621
    Translation

    Target ORF information:

    RefSeq Version XM_017005771.2
    Organism Homo sapiens(human)
    Definition Homo sapiens NIMA related kinase 10 (NEK10), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017005771.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTGATC AAGATAAAAA GGTGAAGACC ACAGAAAAAT CAACTGATAA ACAGCAAGAA 
    ATCACCATCA GGGACTATTC AGATCTTAAA AGACTTCGGT GCCTTTTGAA CGTCCAATCA
    AGCAAACAAC AGCTTCCAGC CATTAACTTC GATAGTGCCC AAAATAGCAT GACGAAGTCT
    GAGCCCGCCA TCAGGGCGGG TGGACACAGA GCTCGGGGTC AGTGGCATGA ATCCACAGAA
    GCTGTTGAAC TTGAAAATTT TAGTATAAAC TACAAGAATG AGAGAAATTT CAGCAAACAT
    CCTCAGCGTA AACTATTTCA GGAGATCTTT ACCGCCTTGG TGAAAAATAG ACTCATAAGC
    AGAGAGTGGG TTAATCGAGC CCCATCTATT CATTTTCTGA GAGTGTTAAT CTGTCTGAGG
    CTACTAATGA GGGATCCATG TTATCAGGAA ATACTCCATA GCTTGGGTGG GATTGAAAAC
    CTAGCTCAGT ATATGGAGAT TGTAGCCAAT GAGTACCTCG GCTATGGAGA AGAGCAGCAC
    ACTGTGGACA AGCTGGTCAA CATGACATAT ATTTTTCAAA AACTTGCTGC AGTCAAAGAT
    CAAAGAGAAT GGGTCACCAC AAGTGGAGCC CACAAGACAT TAGTAAATTT ACTTGGTGCC
    CGAGATACTA ATGTTCTATT GGGTTCCCTT CTGGCTCTGG CTAGTTTAGC AGAAAGTCAA
    GAATGTAGGG AGAAGATAAG TGAACTCAAC ATTGTAGAAA ATCTGTTGAT GATTTTACAT
    GAATATGACT TGCTTTCTAA AAGACTAACA GCGGAGTTGC TGCGCCTACT TTGTGCAGAG
    CCCCAGGTGA AAGAGCAGGT GAAGCTCTAT GAGGGGATAC CGGTCCTCCT CAGTCTGCTC
    CACTCTGACC ACTTGAAGCT CCTCTGGAGC ATTGTCTGGA TTCTGGTACA GGTTTGTGAG
    GACCCTGAGA CCAGCGTGGA AATTCGCATT TGGGGAGGCA TCAAACAGCT TCTTCATATT
    TTACAAGGAG ACAGAAATTT TGTTTCTGAT CACTCCTCCA TTGGAAGCCT GTCCAGTGCA
    AATGCTGCAG GCCGAATCCA GCAGCTTCAT TTATCAGAAG ACTTGAGCCC TAGGGAAATA
    CAAGAAAATA CTTTCTCACT TCAAGCAGCC TGCTGTGCTG CCCTCACTGA GCTGGTGCTC
    AATGACACCA ATGCCCACCA GGTGGTTCAG GAAAATGGTG TATATACAAT AGCAAAATTA
    ATTTTACCAA ATAAGCAAAA GAATGCAGCA AAAAGTAATC TATTACAGTG TTATGCTTTC
    AGAGCCTTGA GATTTCTCTT CAGTATGGAA AGAAACAGAC CACTCTTTAA AAGACTTTTC
    CCCACAGACT TGTTTGAGAT CTTCATTGAC ATAGGGCATT ATGTACGTGA TATCAGTGCT
    TATGAAGAAT TGGTATCCAA GCTGAATTTA TTAGTGGAGG ATGAACTGAA GCAAATTGCT
    GAAAATATTG AAAGCATTAA TCAGAACAAA GCTCCTTTGA AATATATAGG CAACTATGCA
    ATTTTGGATC ATCTTGGAAG TGGAGCTTTT GGCTGTGTTT ACAAGGTTAG AAAGCATAGT
    GGTCAAAATC TTTTAGCAAT GAAAGAGGTC AATTTACATA ACCCAGCATT TGGAAAGGAT
    AAGAAAGATC GAGACAGCAG CGTAAGGAAT ATTGTTTCTG AATTAACAAT AATTAAAGAG
    CAGCTTTATC ATCCCAACAT TGTACGTTAT TACAAAACAT TTCTGGAAAA TGATAGGTTG
    TACATAGTTA TGGAGCTGAT AGAAGGAGCC CCGCTTGGAG AGCATTTCAG TTCTTTGAAG
    GAAAAACATC ACCATTTTAC TGAAGAAAGA CTATGGAAAA TATTTATACA GCTGTGCTTA
    GCTCTTCGAT ACTTACACAA GGAGAAGAGG ATTGTCCATA GAGATCTGAC ACCAAACAAC
    ATTATGTTGG GGGATAAGGA CAAAGTAACC GTTACTGACT TTGGCCTGGC AAAGCAAAAA
    CAAGAAAACA GTAAACTCAC CTCTGTGGTT GGAACAATCC TGTATTCTTG CCCCGAGGTA
    CTGAAGAGTG AGCCGTATGG GGAGAAGGCT GATGTCTGGG CAGTAGGCTG CATCCTTTAT
    CAGATGGCGA CTTTGAGTCC CCCCTTCTAC AGCACTAACA TGCTGTCCTT GGCTACAAAA
    ATAGTGGAGG CGGTATATGA ACCAGTCCCA GAAGGTATCT ACTCTGAAAA AGTAACAGAC
    ACCATCAGCA GGTGCCTCAC TCCTGATGCG GAAGCTCGTC CAGATATTGT AGAAGTCAGT
    TCGATGATAT CAGATGTCAT GATGAAATAT TTAGACAACT TATCTACATC CCAGTTGTCC
    TTGGAAAAGA AGCTAGAACG GGAACGAAGA CGCACACAAA GGTATTTTAT GGAAGCCAAC
    CGGAACACCG TCACATGTCA CCATGAGCTG GCTGTTCTAT CTCACGAGAC CTTTGAGAAG
    GCAAGTTTGA GTAGCAGCAG CAGTGGAGCA GCCAGCCTGA AAAGTGAACT TTCAGAAAGC
    GCAGACCTGC CCCCTGAAGG CTTCCAGGCC TCCTATGGTA AAGACGAAGA CAGGGCCTGT
    GACGAAATCC TGTCAGATGA TAACTTCAAC CTGGAAAATG CTGAGAAAGA TACATATTCA
    GAGGTAGATG ATGAATTGGA CATTTCGGAT AACTCCAGCA GCTCCAGTTC AAGCCCTCTG
    AAAGAATCTA CATTCAACAT TTTAAAGAGA AGTTTTAGTG CTTCAGGAGG AGAAAGACAA
    TCCCAAACAA GGGACTTCAC TGGAGGAACA GGATCAAGAC CAAGACCAGC ATCAGCAGGA
    ATTGCTGTGT CCCAGAGGAA AGTGCGTCAG ATCAGTGATC CTATTCAGCA GATATTAATT
    CAGCTGCACA AAATAATCTA TATCACACAG CTTCCTCCAG CTTTGCACCA CAATTTGAAA
    AGAAGGGTTA TAGAGAGATT CAAGAAATCC CTCTTCAGCC AGCAGAGTAA CCCTTGTAAT
    TTGAAATCTG AAATTAAAAA GTTATCTCAG GGATCTCCAG AACCGATTGA GCCCAACTTT
    TTCACAGCAG ATTACCATTT ATTACATCGT TCATCCGGTG GAAACAGCCT GTCCCCAAAT
    GACCCTACAG GTTTACCAAC CAGCATTGAA TTGGAGGAAG GAATAACATA TGAACAGATG
    CAGACTGTGA TTGAAGAAGT CCTTGAGGAA AGTGGCTATT ACAATTTTAC ATCTAACAGG
    TATCATTCCT ATCCATGGGG GACCAAGAAT CACCCAACCA AAAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    Nek10 mediates G2/M cell cycle arrest and MEK autoactivation in response to UV irradiation.
    Molecular and cellular biology31(1)30-42(2011 Jan)
    Moniz LS,Stambolic V


    Newly discovered breast cancer susceptibility loci on 3p24 and 17q23.2.
    Nature genetics41(5)585-90(2009 May)
    Ahmed S,Thomas G,Ghoussaini M,Healey CS,Humphreys MK,Platte R,Morrison J,Maranian M,Pooley KA,Luben R,Eccles D,Evans DG,Fletcher O,Johnson N,dos Santos Silva I,Peto J,Stratton MR,Rahman N,Jacobs K,Prentice R,Anderson GL,Rajkovic A,Curb JD,Ziegler RG,Berg CD,Buys SS,McCarty CA,Feigelson HS,Calle EE,Thun MJ,Diver WR,Bojesen S,Nordestgaard BG,Flyger H,D?rk T,Sch?rmann P,Hillemanns P,Karstens JH,Bogdanova NV,Antonenkova NN,Zalutsky IV,Bermisheva M,Fedorova S,Khusnutdinova E,,Kang D,Yoo KY,Noh DY,Ahn SH,Devilee P,van Asperen CJ,Tollenaar RA,Seynaeve C,Garcia-Closas M,Lissowska J,Brinton L,Peplonska B,Nevanlinna H,Heikkinen T,Aittom?ki K,Blomqvist C,Hopper JL,Southey MC,Smith L,Spurdle AB,Schmidt MK,Broeks A,van Hien RR,Cornelissen S,Milne RL,Ribas G,Gonz?lez-Neira A,Benitez J,Schmutzler RK,Burwinkel B,Bartram CR,Meindl A,Brauch H,Justenhoven C,Hamann U,,Chang-Claude J,Hein R,Wang-Gohrke S,Lindblom A,Margolin S,Mannermaa A,Kosma VM,Kataja V,Olson JE,Wang X,Fredericksen Z,Giles GG,Severi G,Baglietto L,English DR,Hankinson SE,Cox DG,Kraft P,Vatten LJ,Hveem K,Kumle M,Sigurdson A,Doody M,Bhatti P,Alexander BH,Hooning MJ,van den Ouweland AM,Oldenburg RA,Schutte M,Hall P,Czene K,Liu J,Li Y,Cox A,Elliott G,Brock I,Reed MW,Shen CY,Yu JC,Hsu GC,Chen ST,Anton-Culver H,Ziogas A,Andrulis IL,Knight JA,,,Beesley J,Goode EL,Couch F,Chenevix-Trench G,Hoover RN,Ponder BA,Hunter DJ,Pharoah PD,Dunning AM,Chanock SJ,Easton DF