CRYM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYM
Entrez Gene ID	1428
Full Name	crystallin mu
Synonyms	DFNA40,THBP
General protein information	Preferred Names crystallin mu Names ketimine reductase mu-crystallin NADP-regulated thyroid-hormone binding protein mu-crystallin homolog thiomorpholine-carboxylate dehydrogenase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p12.2
Summary	Crystallins are separated into two classes: taxon-specific and ubiquitous. The former class is also called phylogenetically-restricted crystallins. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. This gene encodes a taxon-specific crystallin protein that binds NADPH and has sequence similarity to bacterial ornithine cyclodeaminases. The encoded protein does not perform a structural role in lens tissue, and instead it binds thyroid hormone for possible regulatory or developmental roles. Mutations in this gene have been associated with autosomal dominant non-syndromic deafness. [provided by RefSeq, Sep 2014].
Disorder MIM:	123740
Disorder Html:	Deafness, autosomal dominant 40, 616357 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001376256.1	NP_001363185.1	ketimine reductase mu-crystallin
XM_024450157.1	XP_024305925.1	ketimine reductase mu-crystallin isoform X1
NM_001888.5	NP_001879.1	ketimine reductase mu-crystallin
NM_001888.4	NP_001879.1	ketimine reductase mu-crystallin

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Crym	NP_446407.1
Bos taurus (cattle)	CRYM	NP_001039379.1
Gallus gallus (chicken)	CRYM	XP_424610.2
Danio rerio (zebrafish)	crym	NP_001074156.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011137	XP_309511.4
Canis lupus familiaris (dog)	CRYM	XP_850454.1
Arabidopsis thaliana (thale cress)	AT5G52810	NP_200093.1
Oryza sativa (rice)	Os10g0533200	NP_001065154.1
Xenopus tropicalis (tropical clawed frog)	crym	NP_001096532.1
Homo sapiens (human)	CRYM	NP_001879.1
Mus musculus (house mouse)	Crym	NP_057878.1
Drosophila melanogaster (fruit fly)	CG4872	NP_573173.2
Schizosaccharomyces pombe (fission yeast)	SPAP11E10.01	NP_593514.1
Pan troglodytes (chimpanzee)	CRYM	XP_001159613.1
	CRYM	XP_001091397.1

Gene Ontology
Bibliography

Related articles in PubMed

Ketimine reductase/CRYM catalyzes reductive alkylamination of ?-keto acids, confirming its function as an imine reductase.
Hallen A, Cooper AJ, Smith JR, Jamie JF, Karuso P
Amino acids47(11)2457-61(2015 Nov)

Insights into Enzyme Catalysis and Thyroid Hormone Regulation of Cerebral Ketimine Reductase/?-Crystallin Under Physiological Conditions.
Hallen A, Cooper AJ, Jamie JF, Karuso P
Neurochemical research40(6)1252-66(2015 Jun)

Adipose tissue ?-crystallin is a thyroid hormone-binding protein associated with systemic insulin sensitivity.
Serrano M, Moreno M, Ortega FJ, Xifra G, Ricart W, Moreno-Navarrete JM, Fern?ndez-Real JM
The Journal of clinical endocrinology and metabolism99(11)E2259-68(2014 Nov)

Identification of CRYM as a candidate responsible for nonsyndromic deafness, through cDNA microarray analysis of human cochlear and vestibular tissues.
Abe S, Katagiri T, Saito-Hisaminato A, Usami S, Inoue Y, Tsunoda T, Nakamura Y
American journal of human genetics72(1)73-82(2003 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings confirm that, mechanistically, ketimine reductase/CRYM acts as a classical imine reductase and may explain the finding of bound pyruvate in the crystallized protein.
Title: Ketimine reductase/CRYM catalyzes reductive alkylamination of ?-keto acids, confirming its function as an imine reductase.

ketimine reductase is a key enzyme in the pipecolate pathway, which is the main lysine degradation pathway in the brain
Title: Insights into Enzyme Catalysis and Thyroid Hormone Regulation of Cerebral Ketimine Reductase/?-Crystallin Under Physiological Conditions.

The expression of mu-crystallin was regulated through the AP-1 site in the promoter.
Title: Possible roles of the AP-1 site in the cytosolic T3 binding protein promoter and insights into its physiological significance.

Its enzyme function has been determined as a ketimine reductase in mammalian brain.
Title: Mammalian forebrain ketimine reductase identified as ?-crystallin; potential regulation by thyroid hormones.

This protein has been found differentially expressed in thalami from patients with schizophrenia.
Title: Proteome analysis of the thalamus and cerebrospinal fluid reveals glycolysis dysfunction and potential biomarkers candidates for schizophrenia.

The following CRYM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17997	NM_001888.5 Latest version!	Homo sapiens crystallin mu (CRYM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu17997	XM_024450157.1 Latest version!	Homo sapiens crystallin mu (CRYM), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu17997	NM_001376256.1 Latest version!	Homo sapiens crystallin mu (CRYM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu17997	NM_001888.4	Homo sapiens crystallin mu (CRYM), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYM ( NM_001888.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001879.1 Homo sapiens crystallin mu (CRYM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17997
Clone ID Related Accession (Same CDS sequence)	NM_001376256.1 , XM_024450157.1 , NM_001888.5 , NM_001888.4
Accession Version	NM_001888.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ketimine reductase mu-crystallin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG033512.1, BX648477.1, AK290852.1 and L02950.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001888.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648477.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001879.1
CDS	278..1222
Translation	MSRVPAFLSAAEVEEHLRSSSLLIPPLETALANFSSGPEGGVMQPVRTVVPVTKHRGYLGVMPAYSAAEDALTTKLVTFYEDRGITSVVPSHQATVLLFEPSNGTLLAVMDGNVITAKRTAAVSAIATKFLKPPSSEVLCILGAGVQAYSHYEIFTEQFSFKEVRIWNRTKENAEKFADTVQGEVRVCSSVQEAVAGADVIITVTLATEPILFGEWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

Target ORF information:

RefSeq Version	NM_001888.5
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin mu (CRYM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001888.5

ORF Insert Sequence:

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYM ( NM_001888.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001879.1 Homo sapiens crystallin mu (CRYM), mRNA.

Price & Availability↑

CloneID	OHu17997
Clone ID Related Accession (Same CDS sequence)	NM_001376256.1 , XM_024450157.1 , NM_001888.5 , NM_001888.4
Accession Version	NM_001888.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ketimine reductase mu-crystallin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG033512.1, BX648477.1, AK290852.1 and L02950.1. This sequence is a reference standard in the RefSeqGene project. On Sep 18, 2014 this sequence version replaced NM_001888.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648477.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001879.1
CDS	352..1296
Translation	MSRVPAFLSAAEVEEHLRSSSLLIPPLETALANFSSGPEGGVMQPVRTVVPVTKHRGYLGVMPAYSAAEDALTTKLVTFYEDRGITSVVPSHQATVLLFEPSNGTLLAVMDGNVITAKRTAAVSAIATKFLKPPSSEVLCILGAGVQAYSHYEIFTEQFSFKEVRIWNRTKENAEKFADTVQGEVRVCSSVQEAVAGADVIITVTLATEPILFGEWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

Target ORF information:

RefSeq Version	NM_001888.4
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin mu (CRYM), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001888.4

ORF Insert Sequence:

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYM ( XM_024450157.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024305925.1 Homo sapiens crystallin mu (CRYM), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17997
Clone ID Related Accession (Same CDS sequence)	NM_001376256.1 , XM_024450157.1 , NM_001888.5 , NM_001888.4
Accession Version	XM_024450157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	ketimine reductase mu-crystallin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024305925.1
CDS	269..1213
Translation	MSRVPAFLSAAEVEEHLRSSSLLIPPLETALANFSSGPEGGVMQPVRTVVPVTKHRGYLGVMPAYSAAEDALTTKLVTFYEDRGITSVVPSHQATVLLFEPSNGTLLAVMDGNVITAKRTAAVSAIATKFLKPPSSEVLCILGAGVQAYSHYEIFTEQFSFKEVRIWNRTKENAEKFADTVQGEVRVCSSVQEAVAGADVIITVTLATEPILFGEWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

Target ORF information:

RefSeq Version	XM_024450157.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin mu (CRYM), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450157.1

ORF Insert Sequence:

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYM ( NM_001376256.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001363185.1 Homo sapiens crystallin mu (CRYM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17997
Clone ID Related Accession (Same CDS sequence)	NM_001376256.1 , XM_024450157.1 , NM_001888.5 , NM_001888.4
Accession Version	NM_001376256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-02
Organism	Homo sapiens(human)
Product	ketimine reductase mu-crystallin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290852.1 and BX648477.1. On Nov 13, 2019 this sequence version replaced XM_024450157.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC018061.1, L02950.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001363185.1
CDS	40..984
Translation	MSRVPAFLSAAEVEEHLRSSSLLIPPLETALANFSSGPEGGVMQPVRTVVPVTKHRGYLGVMPAYSAAEDALTTKLVTFYEDRGITSVVPSHQATVLLFEPSNGTLLAVMDGNVITAKRTAAVSAIATKFLKPPSSEVLCILGAGVQAYSHYEIFTEQFSFKEVRIWNRTKENAEKFADTVQGEVRVCSSVQEAVAGADVIITVTLATEPILFGEWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDSQEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDTVAAKLIYDSWSSGK

Target ORF information:

RefSeq Version	NM_001376256.1
Organism	Homo sapiens(human)
Definition	Homo sapiens crystallin mu (CRYM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001376256.1

ORF Insert Sequence:

ATGAGCCGGG TACCAGCGTT CCTGAGCGCG GCCGAGGTGG AGGAACACCT CCGCAGCTCC 
AGCCTCCTCA TCCCGCCTCT AGAGACGGCC CTGGCCAACT TCTCCAGCGG TCCCGAAGGA 
GGGGTCATGC AGCCCGTGCG CACCGTGGTG CCGGTGACCA AGCACAGGGG CTACCTGGGG 
GTCATGCCCG CCTACAGTGC TGCAGAGGAT GCACTGACCA CCAAGTTGGT CACCTTCTAC 
GAGGACCGCG GCATCACCTC GGTCGTCCCT TCCCACCAGG CTACTGTGCT ACTCTTTGAG 
CCCAGCAATG GCACCCTGCT GGCGGTCATG GATGGAAATG TCATAACTGC AAAGAGAACA 
GCTGCAGTTT CTGCCATTGC CACCAAGTTT CTGAAACCTC CCAGCAGTGA AGTGCTGTGC 
ATCCTTGGGG CTGGGGTCCA GGCCTACAGC CATTATGAGA TCTTCACAGA GCAGTTCTCC 
TTTAAGGAGG TGAGGATATG GAACCGCACC AAAGAAAATG CAGAGAAGTT TGCAGACACA 
GTGCAAGGAG AGGTACGGGT CTGTTCTTCG GTCCAGGAGG CTGTGGCAGG TGCAGATGTG 
ATCATCACAG TCACCCTGGC AACAGAGCCC ATTTTGTTTG GTGAATGGGT GAAGCCAGGG 
GCTCACATCA ATGCTGTTGG AGCCAGCAGA CCTGACTGGA GAGAACTGGA TGATGAGCTC 
ATGAAAGAAG CTGTGCTGTA CGTGGATTCC CAGGAGGCTG CCCTGAAGGA GTCTGGAGAT 
GTCCTGCTGT CAGGGGCCGA GATCTTTGCT GAGCTGGGAG AAGTGATTAA GGGAGTGAAA 
CCAGCCCACT GTGAGAAGAC CACCGTGTTC AAGTCTTTGG GAATGGCAGT GGAAGACACA 
GTTGCAGCCA AACTCATCTA TGATTCCTGG TCATCTGGTA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ketimine reductase/CRYM catalyzes reductive alkylamination of ?-keto acids, confirming its function as an imine reductase.
Amino acids47(11)2457-61(2015 Nov)
Hallen A,Cooper AJ,Smith JR,Jamie JF,Karuso P

Insights into Enzyme Catalysis and Thyroid Hormone Regulation of Cerebral Ketimine Reductase/?-Crystallin Under Physiological Conditions.
Neurochemical research40(6)1252-66(2015 Jun)
Hallen A,Cooper AJ,Jamie JF,Karuso P

Adipose tissue ?-crystallin is a thyroid hormone-binding protein associated with systemic insulin sensitivity.
The Journal of clinical endocrinology and metabolism99(11)E2259-68(2014 Nov)
Serrano M,Moreno M,Ortega FJ,Xifra G,Ricart W,Moreno-Navarrete JM,Fern?ndez-Real JM

Identification of CRYM as a candidate responsible for nonsyndromic deafness, through cDNA microarray analysis of human cochlear and vestibular tissues.
American journal of human genetics72(1)73-82(2003 Jan)
Abe S,Katagiri T,Saito-Hisaminato A,Usami S,Inoue Y,Tsunoda T,Nakamura Y

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CRYM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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