COX5B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	COX5B
Entrez Gene ID	1329
Full Name	cytochrome c oxidase subunit 5B
Synonyms	COXVB
General protein information	Preferred Names cytochrome c oxidase subunit 5B Names cytochrome c oxidase subunit 5B, mitochondrial cytochrome c oxidase polypeptide VB, mitochondrial cytochrome c oxidase subunit Vb
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Summary	Cytochrome C oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Vb of the human mitochondrial respiratory chain enzyme. [provided by RefSeq, Jul 2008].
Disorder MIM:	123866

mRNA and Protein(s)

mRNA	Protein	Name
NM_001862.3	NP_001853.2	cytochrome c oxidase subunit 5B, mitochondrial precursor
NM_001862.2	NP_001853.2	cytochrome c oxidase subunit 5B, mitochondrial precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LOC741789	XP_001154903.2
	LOC707227	XP_001100566.1
Rattus norvegicus (Norway rat)	Cox5b	NP_446038.1
Danio rerio (zebrafish)	LOC100002384	XP_001342184.1
	LOC710274	XP_001098868.1
Xenopus tropicalis (tropical clawed frog)	cox5b.2	XP_004915951.1
Homo sapiens (human)	COX5B	NP_001853.2
	LOC701661	XP_001099921.1
Canis lupus familiaris (dog)	COX5B	NP_001137602.1
Bos taurus (cattle)	COX5B	NP_001029218.1

Gene Ontology
Bibliography

Related articles in PubMed

Topography of human cytochrome b
Samhan-Arias AK, Almeida RM, Ramos S, Cordas CM, Moura I, Gutierrez-Merino C, Moura JJG
Biochimica et biophysica acta1859(2)78-87(2018 Feb)

High expression of COX5B is associated with poor prognosis in breast cancer.
Gao SP, Sun HF, Fu WY, Li LD, Zhao Y, Chen MT, Jin W
Future oncology (London, England)13(19)1711-1719(2017 Aug)

Loss of COX5B inhibits proliferation and promotes senescence via mitochondrial dysfunction in breast cancer.
Gao SP, Sun HF, Jiang HL, Li LD, Hu X, Xu XE, Jin W
Oncotarget6(41)43363-74(2015 Dec)

Cytochrome c oxidase is activated by the oncoprotein Ras and is required for A549 lung adenocarcinoma growth.
Telang S, Nelson KK, Siow DL, Yalcin A, Thornburg JM, Imbert-Fernandez Y, Klarer AC, Farghaly H, Clem BF, Eaton JW, Chesney J
Molecular cancer1160(2012 Aug)

Next-generation sequencing to generate interactome datasets.
Yu H, Tardivo L, Tam S, Weiner E, Gebreab F, Fan C, Svrzikapa N, Hirozane-Kishikawa T, Rietman E, Yang X, Sahalie J, Salehi-Ashtiani K, Hao T, Cusick ME, Hill DE, Roth FP, Braun P, Vidal M
Nature methods8(6)478-80(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

silence of COX5B leads to metabolic disorders, such as increased glucose uptake and decreased lactate secretion
Title: Loss of COX5B inhibits proliferation and promotes senescence via mitochondrial dysfunction in breast cancer.

Transfected the A549 cells with COX Vb small interfering or shRNA and observed a significant reduction of their COX activity, oxygen consumption, ATP and ability to grow in soft agar and as poorly differentiated tumors in athymic mice.
Title: Cytochrome c oxidase is activated by the oncoprotein Ras and is required for A549 lung adenocarcinoma growth.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association of genetic variants with hemorrhagic stroke in Japanese individuals.

Observational study of gene-disease association. (HuGE Navigator)
Title: Assessment of a polymorphism of SDK1 with hypertension in Japanese Individuals.

The following COX5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COX5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30507	NM_001862.3 Latest version!	Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30507	NM_001862.2	Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COX5B ( NM_001862.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001853.2 Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.

Price & Availability↑

CloneID	OHu30507
Clone ID Related Accession (Same CDS sequence)	NM_001862.3 , NM_001862.2
Accession Version	NM_001862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cytochrome c oxidase subunit 5B, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006229.2 and AC017099.11. On May 17, 2019 this sequence version replaced NM_001862.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CB110753.1, CB109633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000258424.3/ ENSP00000258424.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GGTTACTTCG CGGAGCTGGA ACGCTGGCCG CGCAGGCCCT GAGGGCTCGC 
GGCCCCAGTG GCGCGGCCGC GATGCGCTCC ATGGCATCTG GAGGTGGTGT TCCCACTGAT 
GAAGAGCAGG CGACTGGGTT GGAGAGGGAG ATCATGCTGG CTGCAAAGAA GGGACTGGAC 
CCATACAATG TACTGGCCCC AAAGGGAGCT TCAGGCACCA GGGAAGACCC TAATTTAGTC 
CCCTCCATCT CCAACAAGAG AATAGTAGGC TGCATCTGTG AAGAGGACAA TACCAGCGTC 
GTCTGGTTTT GGCTGCACAA AGGCGAGGCC CAGCGATGCC CCCGCTGTGG AGCCCATTAC 
AAGCTGGTGC CCCAGCAGCT GGCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001853.2
CDS	26..415
Translation	MASRLLRGAGTLAAQALRARGPSGAAAMRSMASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH

Target ORF information:

RefSeq Version	NM_001862.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001862.3

ORF Insert Sequence:

ATGGCTTCAA GGTTACTTCG CGGAGCTGGA ACGCTGGCCG CGCAGGCCCT GAGGGCTCGC 
GGCCCCAGTG GCGCGGCCGC GATGCGCTCC ATGGCATCTG GAGGTGGTGT TCCCACTGAT 
GAAGAGCAGG CGACTGGGTT GGAGAGGGAG ATCATGCTGG CTGCAAAGAA GGGACTGGAC 
CCATACAATG TACTGGCCCC AAAGGGAGCT TCAGGCACCA GGGAAGACCC TAATTTAGTC 
CCCTCCATCT CCAACAAGAG AATAGTAGGC TGCATCTGTG AAGAGGACAA TACCAGCGTC 
GTCTGGTTTT GGCTGCACAA AGGCGAGGCC CAGCGATGCC CCCGCTGTGG AGCCCATTAC 
AAGCTGGTGC CCCAGCAGCT GGCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COX5B ( NM_001862.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001853.2 Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.

Price & Availability↑

CloneID	OHu30507
Clone ID Related Accession (Same CDS sequence)	NM_001862.3 , NM_001862.2
Accession Version	NM_001862.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	cytochrome c oxidase subunit 5B, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006229.2. On Nov 20, 2001 this sequence version replaced NM_001862.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006229.2, SRR1163657.268207.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCAA GGTTACTTCG CGGAGCTGGA ACGCTGGCCG CGCAGGCCCT GAGGGCTCGC 
GGCCCCAGTG GCGCGGCCGC GATGCGCTCC ATGGCATCTG GAGGTGGTGT TCCCACTGAT 
GAAGAGCAGG CGACTGGGTT GGAGAGGGAG ATCATGCTGG CTGCAAAGAA GGGACTGGAC 
CCATACAATG TACTGGCCCC AAAGGGAGCT TCAGGCACCA GGGAAGACCC TAATTTAGTC 
CCCTCCATCT CCAACAAGAG AATAGTAGGC TGCATCTGTG AAGAGGACAA TACCAGCGTC 
GTCTGGTTTT GGCTGCACAA AGGCGAGGCC CAGCGATGCC CCCGCTGTGG AGCCCATTAC 
AAGCTGGTGC CCCAGCAGCT GGCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001853.2
CDS	30..419
Translation	MASRLLRGAGTLAAQALRARGPSGAAAMRSMASGGGVPTDEEQATGLEREIMLAAKKGLDPYNVLAPKGASGTREDPNLVPSISNKRIVGCICEEDNTSVVWFWLHKGEAQRCPRCGAHYKLVPQQLAH

Target ORF information:

RefSeq Version	NM_001862.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome c oxidase subunit 5B (COX5B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001862.2

ORF Insert Sequence:

ATGGCTTCAA GGTTACTTCG CGGAGCTGGA ACGCTGGCCG CGCAGGCCCT GAGGGCTCGC 
GGCCCCAGTG GCGCGGCCGC GATGCGCTCC ATGGCATCTG GAGGTGGTGT TCCCACTGAT 
GAAGAGCAGG CGACTGGGTT GGAGAGGGAG ATCATGCTGG CTGCAAAGAA GGGACTGGAC 
CCATACAATG TACTGGCCCC AAAGGGAGCT TCAGGCACCA GGGAAGACCC TAATTTAGTC 
CCCTCCATCT CCAACAAGAG AATAGTAGGC TGCATCTGTG AAGAGGACAA TACCAGCGTC 
GTCTGGTTTT GGCTGCACAA AGGCGAGGCC CAGCGATGCC CCCGCTGTGG AGCCCATTAC 
AAGCTGGTGC CCCAGCAGCT GGCACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Topography of human cytochrome b
Biochimica et biophysica acta1859(2)78-87(2018 Feb)
Samhan-Arias AK,Almeida RM,Ramos S,Cordas CM,Moura I,Gutierrez-Merino C,Moura JJG

High expression of COX5B is associated with poor prognosis in breast cancer.
Future oncology (London, England)13(19)1711-1719(2017 Aug)
Gao SP,Sun HF,Fu WY,Li LD,Zhao Y,Chen MT,Jin W

Loss of COX5B inhibits proliferation and promotes senescence via mitochondrial dysfunction in breast cancer.
Oncotarget6(41)43363-74(2015 Dec)
Gao SP,Sun HF,Jiang HL,Li LD,Hu X,Xu XE,Jin W

Cytochrome c oxidase is activated by the oncoprotein Ras and is required for A549 lung adenocarcinoma growth.
Molecular cancer1160(2012 Aug)
Telang S,Nelson KK,Siow DL,Yalcin A,Thornburg JM,Imbert-Fernandez Y,Klarer AC,Farghaly H,Clem BF,Eaton JW,Chesney J

Next-generation sequencing to generate interactome datasets.
Nature methods8(6)478-80(2011 Jun)
Yu H,Tardivo L,Tam S,Weiner E,Gebreab F,Fan C,Svrzikapa N,Hirozane-Kishikawa T,Rietman E,Yang X,Sahalie J,Salehi-Ashtiani K,Hao T,Cusick ME,Hill DE,Roth FP,Braun P,Vidal M

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COX5B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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