GLYCTK cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLYCTK
Entrez Gene ID	132158
Full Name	glycerate kinase
Synonyms	HBEBP2,HBEBP4,HBeAgBP4A
General protein information	Preferred Names glycerate kinase Names glycerate kinase HBeAg binding protein 4 HBeAg-binding protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.2
Summary	This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jan 2009].
Disorder MIM:	610516
Disorder Html:	D-glyceric aciduria, 220120 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_145262.4	NP_660305.2	glycerate kinase isoform 1
XM_024453352.1	XP_024309120.1	glycerate kinase isoform X3
NM_001144951.1	NP_001138423.1	glycerate kinase isoform 2
XM_017005730.1	XP_016861219.1	glycerate kinase isoform X2
NM_145262.3	NP_660305.2	glycerate kinase isoform 1
XM_024453351.1	XP_024309119.1	glycerate kinase isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	GLYCTK	XP_004944540.1
Homo sapiens (human)	GLYCTK	NP_660305.2
Xenopus tropicalis (tropical clawed frog)	glyctk	NP_001072628.1
Pan troglodytes (chimpanzee)	GLYCTK	XP_001171685.1
Bos taurus (cattle)	GLYCTK	NP_001039452.1
Rattus norvegicus (Norway rat)	Glyctk	NP_001102919.1
Drosophila melanogaster (fruit fly)	CG9886	NP_001259941.1
Caenorhabditis elegans (roundworm)	C13B9.2	NP_498462.3
Canis lupus familiaris (dog)	GLYCTK	XP_005632516.1
Mus musculus (house mouse)	Glyctk	NP_001034675.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007988	XP_001689038.1
Danio rerio (zebrafish)	glyctk	NP_001070856.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association analysis identifies 13 new risk loci for schizophrenia.
Ripke S, O'Dushlaine C, Chambert K, Moran JL, K?hler AK, Akterin S, Bergen SE, Collins AL, Crowley JJ, Fromer M, Kim Y, Lee SH, Magnusson PK, Sanchez N, Stahl EA, Williams S, Wray NR, Xia K, Bettella F, Borglum AD, Bulik-Sullivan BK, Cormican P, Craddock N, de Leeuw C, Durmishi N, Gill M, Golimbet V, Hamshere ML, Holmans P, Hougaard DM, Kendler KS, Lin K, Morris DW, Mors O, Mortensen PB, Neale BM, O'Neill FA, Owen MJ, Milovancevic MP, Posthuma D, Powell J, Richards AL, Riley BP, Ruderfer D, Rujescu D, Sigurdsson E, Silagadze T, Smit AB, Stefansson H, Steinberg S, Suvisaari J, Tosato S, Verhage M, Walters JT, , Levinson DF, Gejman PV, Kendler KS, Laurent C, Mowry BJ, O'Donovan MC, Owen MJ, Pulver AE, Riley BP, Schwab SG, Wildenauer DB, Dudbridge F, Holmans P, Shi J, Albus M, Alexander M, Campion D, Cohen D, Dikeos D, Duan J, Eichhammer P, Godard S, Hansen M, Lerer FB, Liang KY, Maier W, Mallet J, Nertney DA, Nestadt G, Norton N, O'Neill FA, Papadimitriou GN, Ribble R, Sanders AR, Silverman JM, Walsh D, Williams NM, Wormley B, , Arranz MJ, Bakker S, Bender S, Bramon E, Collier D, Crespo-Facorro B, Hall J, Iyegbe C, Jablensky A, Kahn RS, Kalaydjieva L, Lawrie S, Lewis CM, Lin K, Linszen DH, Mata I, McIntosh A, Murray RM, Ophoff RA, Powell J, Rujescu D, Van Os J, Walshe M, Weisbrod M, Wiersma D, , Donnelly P, Barroso I, Blackwell JM, Bramon E, Brown MA, Casas JP, Corvin AP, Deloukas P, Duncanson A, Jankowski J, Markus HS, Mathew CG, Palmer CN, Plomin R, Rautanen A, Sawcer SJ, Trembath RC, Viswanathan AC, Wood NW, Spencer CC, Band G, Bellenguez C, Freeman C, Hellenthal G, Giannoulatou E, Pirinen M, Pearson RD, Strange A, Su Z, Vukcevic D, Donnelly P, Langford C, Hunt SE, Edkins S, Gwilliam R, Blackburn H, Bumpstead SJ, Dronov S, Gillman M, Gray E, Hammond N, Jayakumar A, McCann OT, Liddle J, Potter SC, Ravindrarajah R, Ricketts M, Tashakkori-Ghanbaria A, Waller MJ, Weston P, Widaa S, Whittaker P, Barroso I, Deloukas P, Mathew CG, Blackwell JM, Brown MA, Corvin AP, McCarthy MI, Spencer CC, Bramon E, Corvin AP, O'Donovan MC, Stefansson K, Scolnick E, Purcell S, McCarroll SA, Sklar P, Hultman CM, Sullivan PF
Nature genetics45(10)1150-9(2013 Oct)

Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.

Nature genetics43(10)977-83(2011 Sep)

D-glyceric aciduria is caused by genetic deficiency of D-glycerate kinase (GLYCTK).
Sass JO, Fischer K, Wang R, Christensen E, Scholl-B?rgi S, Chang R, Kapelari K, Walter M
Human mutation31(12)1280-5(2010 Dec)

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanch? H, Schreiber S, Nebel A
Mutation research694(1-2)13-9(2010 Dec)

An empirical framework for binary interactome mapping.
Venkatesan K, Rual JF, Vazquez A, Stelzl U, Lemmens I, Hirozane-Kishikawa T, Hao T, Zenkner M, Xin X, Goh KI, Yildirim MA, Simonis N, Heinzmann K, Gebreab F, Sahalie JM, Cevik S, Simon C, de Smet AS, Dann E, Smolyar A, Vinayagam A, Yu H, Szeto D, Borick H, Dricot A, Klitgord N, Murray RR, Lin C, Lalowski M, Timm J, Rau K, Boone C, Braun P, Cusick ME, Roth FP, Hill DE, Tavernier J, Wanker EE, Barab?si AL, Vidal M
Nature methods6(1)83-90(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in the GLYCTK gene is the cause of D-glycerate kinase deficiency and D-glyceric aciduria.
Title: D-glyceric aciduria is caused by genetic deficiency of D-glycerate kinase (GLYCTK).

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.

Identification of two variants of the human glycerate kinase gene-Glycerate kinase 1 (GLYCTK1), longer variant, and Glycerate kinase 2 (GLYCTK2), shorter variant.
Title: Isolation and characterization of the human D-glyceric acidemia related glycerate kinase gene GLYCTK1 and its alternatively splicing variant GLYCTK2.

The following GLYCTK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLYCTK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20837	NM_145262.4 Latest version!	Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu20837	XM_024453351.1 Latest version!	Homo sapiens glycerate kinase (GLYCTK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14700	NM_001144951.1 Latest version!	Homo sapiens glycerate kinase (GLYCTK), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu14700	XM_024453352.1 Latest version!	Homo sapiens glycerate kinase (GLYCTK), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu93814	XM_017005730.1 Latest version!	Homo sapiens glycerate kinase (GLYCTK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu20837	NM_145262.3	Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLYCTK ( NM_145262.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_660305.2 Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20837
Clone ID Related Accession (Same CDS sequence)	NM_145262.4 , NM_145262.3 , XM_024453351.1
Accession Version	NM_145262.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	glycerate kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036862.1, BC042151.1, DA586797.1, AC092045.2, BM555864.1, AI380271.1 and AA100208.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_145262.3. Transcript Variant: This variant (1) encodes the longer isoform (1), also known as GLYCTK1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC042151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000436784.7/ ENSP00000389175.2 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660305.2
CDS	89..1660
Translation	MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

Target ORF information:

RefSeq Version	NM_145262.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145262.4

ORF Insert Sequence:

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLYCTK ( NM_145262.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_660305.2 Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20837
Clone ID Related Accession (Same CDS sequence)	NM_145262.4 , NM_145262.3 , XM_024453351.1
Accession Version	NM_145262.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	glycerate kinase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036862.1, BC042151.1, DA586797.1, AC092045.2, BM555864.1, AI380271.1 and AA100208.1. This sequence is a reference standard in the RefSeqGene project. On Feb 3, 2009 this sequence version replaced NM_145262.2. Transcript Variant: This variant (1) encodes the longer isoform (1), also known as GLYCTK1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC042151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660305.2
CDS	97..1668
Translation	MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

Target ORF information:

RefSeq Version	NM_145262.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145262.3

ORF Insert Sequence:

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLYCTK ( XM_024453351.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309119.1 Homo sapiens glycerate kinase (GLYCTK), transcript variant X1, mRNA.

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CloneID	OHu20837
Clone ID Related Accession (Same CDS sequence)	NM_145262.4 , NM_145262.3 , XM_024453351.1
Accession Version	XM_024453351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycerate kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309119.1
CDS	1903..3474
Translation	MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

Target ORF information:

RefSeq Version	XM_024453351.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453351.1

ORF Insert Sequence:

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGGGGTTCA 
GCTCTGCTGC CTGCCCCCAT CCCACCTGTC ACACTGGAGG AGAAGCAGAC ACTCACTAGA 
CTGCTGGCAG CCCGTGGAGC CACCATCCAG GAGTTGAACA CCATTCGGAA GGCCCTGTCC 
CAGCTCAAGG GTGGGGGGCT GGCTCAGGCC GCCTACCCTG CCCAGGTGGT GAGCCTCATC 
CTGTCAGATG TGGTGGGGGA CCCTGTGGAG GTGATTGCCA GTGGCCCCAC CGTGGCCAGT 
TCCCACAATG TGCAAGATTG CCTGCATATC CTCAATCGCT ACGGCCTCCG TGCAGCCCTG 
CCACGTTCTG TGAAGACTGT GCTGTCTCGG GCCGACTCTG ACCCCCATGG GCCACACACC 
TGTGGCCATG TCCTGAATGT GATCATTGGC TCTAATGTGC TGGCGCTAGC TGAGGCCCAG 
CGGCAGGCCG AGGCACTGGG CTACCAGGCT GTGGTGCTGA GTGCAGCCAT GCAAGGTGAT 
GTAAAAAGTA TGGCCCAGTT CTACGGGCTG CTGGCCCATG TGGCTAGAAC CCGCCTCACC 
CCATCCATGG CTGGGGCTTC TGTGGAGGAA GATGCACAGC TCCATGAGCT GGCAGCTGAG 
CTTCAGATCC CAGACCTGCA GCTGGAGGAG GCTCTGGAGA CCATGGCATG GGGAAGGGGC 
CCAGTCTGCC TGCTGGCTGG TGGCGAGCCC ACAGTACAGC TGCAGGGCTC GGGCAGGGGT 
GGCCGGAACC AGGAACTGGC CCTGCGTGTT GGAGCAGAGT TGAGAAGGTG GCCGCTGGGG 
CCGATAGATG TGCTGTTTTT GAGCGGTGGC ACCGATGGGC AGGATGGGCC CACAGAGGCT 
GCTGGGGCCT GGGTCACACC TGAGCTTGCC AGCCAGGCTG CAGCTGAGGG CCTGGACATA 
GCCACCTTCC TAGCCCACAA TGACTCACAT ACCTTCTTCT GCTGCCTCCA GGGTGGGGCA 
CACCTGCTGC ACACAGGGAT GACAGGTACC AATGTCATGG ACACCCACCT CTTGTTCCTG 
CGGCCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLYCTK ( NM_001144951.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138423.1 Homo sapiens glycerate kinase (GLYCTK), transcript variant 2, mRNA.

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CloneID	OHu14700
Clone ID Related Accession (Same CDS sequence)	XM_024453352.1 , NM_001144951.1
Accession Version	NM_001144951.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	glycerate kinase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036862.1, AY295075.1 and AC092045.2. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift and an early stop codon, compared to variant 1. The encoded isoform (2), also known as GLYCTK2, is shorter and contains a distinct C-terminus compared to isoform 1. ##Evidence-Data-START## CDS exon combination :: AY295075.1, BX462999.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGTGAGCCT 
CATCCTGTCA GATGTGGTGG GGGACCCTGT GGAGGTGATT GCCAGTGGCC CCACCGTGGC 
CAGTTCCCAC AATGTGCAAG ATTGCCTGCA TATCCTCAAT CGCTACGGCC TCCGTGCAGC 
CCTGCCACGT TCTGTGAAGA CTGTGCTGTC TCGGGCCGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138423.1
CDS	97..801
Translation	MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGEPHPVRCGGGPCGGDCQWPHRGQFPQCARLPAYPQSLRPPCSPATFCEDCAVSGRL

Target ORF information:

RefSeq Version	NM_001144951.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001144951.1

ORF Insert Sequence:

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGTGAGCCT 
CATCCTGTCA GATGTGGTGG GGGACCCTGT GGAGGTGATT GCCAGTGGCC CCACCGTGGC 
CAGTTCCCAC AATGTGCAAG ATTGCCTGCA TATCCTCAAT CGCTACGGCC TCCGTGCAGC 
CCTGCCACGT TCTGTGAAGA CTGTGCTGTC TCGGGCCGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLYCTK ( XM_024453352.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309120.1 Homo sapiens glycerate kinase (GLYCTK), transcript variant X5, mRNA.

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CloneID	OHu14700
Clone ID Related Accession (Same CDS sequence)	XM_024453352.1 , NM_001144951.1
Accession Version	XM_024453352.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 705bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycerate kinase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGTGAGCCT 
CATCCTGTCA GATGTGGTGG GGGACCCTGT GGAGGTGATT GCCAGTGGCC CCACCGTGGC 
CAGTTCCCAC AATGTGCAAG ATTGCCTGCA TATCCTCAAT CGCTACGGCC TCCGTGCAGC 
CCTGCCACGT TCTGTGAAGA CTGTGCTGTC TCGGGCCGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309120.1
CDS	1905..2609
Translation	MAAALQVLPRLARAPLHPLLWRGSVARLASSMALAEQARQLFESAVGAVLPGPMLHRALSLDPGGRQLKVRDRNFQLRQNLYLVGFGKAVLGMAAAAEELLGQHLVQGVISVPKGIRAAMERAGKQEMLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGEPHPVRCGGGPCGGDCQWPHRGQFPQCARLPAYPQSLRPPCSPATFCEDCAVSGRL

Target ORF information:

RefSeq Version	XM_024453352.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453352.1

ORF Insert Sequence:

ATGGCTGCAG CCCTGCAGGT CCTGCCCCGC TTGGCCCGAG CCCCCTTGCA TCCACTCCTC 
TGGCGGGGCT CAGTGGCCCG TCTGGCCAGC AGCATGGCCT TGGCAGAGCA GGCCAGGCAG 
CTGTTTGAGA GTGCTGTAGG TGCAGTGCTG CCGGGCCCCA TGCTGCACCG GGCACTATCC 
TTGGACCCTG GTGGCAGACA GCTGAAGGTG CGGGACCGGA ACTTTCAGCT GAGGCAAAAC 
CTCTACCTGG TGGGCTTTGG CAAGGCTGTG CTGGGTATGG CAGCTGCAGC TGAGGAACTA 
CTGGGCCAGC ATCTTGTGCA GGGCGTGATC AGCGTTCCCA AGGGGATCCG TGCTGCCATG 
GAGCGTGCCG GCAAGCAGGA GATGCTGCTG AAGCCACATA GCCGTGTCCA GGTATTCGAG 
GGTGCGGAGG ACAACCTCCC GGACCGCGAT GCGCTGCGGG CTGCACTGGC CATCCAGCAA 
CTGGCTGAGG GACTCACAGC TGATGACCTG CTGCTCGTGC TGATCTCAGG TGGTGAGCCT 
CATCCTGTCA GATGTGGTGG GGGACCCTGT GGAGGTGATT GCCAGTGGCC CCACCGTGGC 
CAGTTCCCAC AATGTGCAAG ATTGCCTGCA TATCCTCAAT CGCTACGGCC TCCGTGCAGC 
CCTGCCACGT TCTGTGAAGA CTGTGCTGTC TCGGGCCGAC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLYCTK ( XM_017005730.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861219.1 Homo sapiens glycerate kinase (GLYCTK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu93814
Clone ID Related Accession (Same CDS sequence)	XM_017005730.1
Accession Version	XM_017005730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	glycerate kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCTGA AGCCACATAG CCGTGTCCAG GTATTCGAGG GTGCGGAGGA CAACCTCCCG 
GACCGCGATG CGCTGCGGGC TGCACTGGCC ATCCAGCAAC TGGCTGAGGG ACTCACAGCT 
GATGACCTGC TGCTCGTGCT GATCTCAGGT GGGGGTTCAG CTCTGCTGCC TGCCCCCATC 
CCACCTGTCA CACTGGAGGA GAAGCAGACA CTCACTAGAC TGCTGGCAGC CCGTGGAGCC 
ACCATCCAGG AGTTGAACAC CATTCGGAAG GCCCTGTCCC AGCTCAAGGG TGGGGGGCTG 
GCTCAGGCCG CCTACCCTGC CCAGGTGGTG AGCCTCATCC TGTCAGATGT GGTGGGGGAC 
CCTGTGGAGG TGATTGCCAG TGGCCCCACC GTGGCCAGTT CCCACAATGT GCAAGATTGC 
CTGCATATCC TCAATCGCTA CGGCCTCCGT GCAGCCCTGC CACGTTCTGT GAAGACTGTG 
CTGTCTCGGG CCGACTCTGA CCCCCATGGG CCACACACCT GTGGCCATGT CCTGAATGTG 
ATCATTGGCT CTAATGTGCT GGCGCTAGCT GAGGCCCAGC GGCAGGCCGA GGCACTGGGC 
TACCAGGCTG TGGTGCTGAG TGCAGCCATG CAAGGTGATG TAAAAAGTAT GGCCCAGTTC 
TACGGGCTGC TGGCCCATGT GGCTAGAACC CGCCTCACCC CATCCATGGC TGGGGCTTCT 
GTGGAGGAAG ATGCACAGCT CCATGAGCTG GCAGCTGAGC TTCAGATCCC AGACCTGCAG 
CTGGAGGAGG CTCTGGAGAC CATGGCATGG GGAAGGGGCC CAGTCTGCCT GCTGGCTGGT 
GGCGAGCCCA CAGTACAGCT GCAGGGCTCG GGCAGGGGTG GCCGGAACCA GGAACTGGCC 
CTGCGTGTTG GAGCAGAGTT GAGAAGGTGG CCGCTGGGGC CGATAGATGT GCTGTTTTTG 
AGCGGTGGCA CCGATGGGCA GGATGGGCCC ACAGAGGCTG CTGGGGCCTG GGTCACACCT 
GAGCTTGCCA GCCAGGCTGC AGCTGAGGGC CTGGACATAG CCACCTTCCT AGCCCACAAT 
GACTCACATA CCTTCTTCTG CTGCCTCCAG GGTGGGGCAC ACCTGCTGCA CACAGGGATG 
ACAGGTACCA ATGTCATGGA CACCCACCTC TTGTTCCTGC GGCCTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861219.1
CDS	324..1514
Translation	MLLKPHSRVQVFEGAEDNLPDRDALRAALAIQQLAEGLTADDLLLVLISGGGSALLPAPIPPVTLEEKQTLTRLLAARGATIQELNTIRKALSQLKGGGLAQAAYPAQVVSLILSDVVGDPVEVIASGPTVASSHNVQDCLHILNRYGLRAALPRSVKTVLSRADSDPHGPHTCGHVLNVIIGSNVLALAEAQRQAEALGYQAVVLSAAMQGDVKSMAQFYGLLAHVARTRLTPSMAGASVEEDAQLHELAAELQIPDLQLEEALETMAWGRGPVCLLAGGEPTVQLQGSGRGGRNQELALRVGAELRRWPLGPIDVLFLSGGTDGQDGPTEAAGAWVTPELASQAAAEGLDIATFLAHNDSHTFFCCLQGGAHLLHTGMTGTNVMDTHLLFLRPR

Target ORF information:

RefSeq Version	XM_017005730.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycerate kinase (GLYCTK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005730.1

ORF Insert Sequence:

ATGCTGCTGA AGCCACATAG CCGTGTCCAG GTATTCGAGG GTGCGGAGGA CAACCTCCCG 
GACCGCGATG CGCTGCGGGC TGCACTGGCC ATCCAGCAAC TGGCTGAGGG ACTCACAGCT 
GATGACCTGC TGCTCGTGCT GATCTCAGGT GGGGGTTCAG CTCTGCTGCC TGCCCCCATC 
CCACCTGTCA CACTGGAGGA GAAGCAGACA CTCACTAGAC TGCTGGCAGC CCGTGGAGCC 
ACCATCCAGG AGTTGAACAC CATTCGGAAG GCCCTGTCCC AGCTCAAGGG TGGGGGGCTG 
GCTCAGGCCG CCTACCCTGC CCAGGTGGTG AGCCTCATCC TGTCAGATGT GGTGGGGGAC 
CCTGTGGAGG TGATTGCCAG TGGCCCCACC GTGGCCAGTT CCCACAATGT GCAAGATTGC 
CTGCATATCC TCAATCGCTA CGGCCTCCGT GCAGCCCTGC CACGTTCTGT GAAGACTGTG 
CTGTCTCGGG CCGACTCTGA CCCCCATGGG CCACACACCT GTGGCCATGT CCTGAATGTG 
ATCATTGGCT CTAATGTGCT GGCGCTAGCT GAGGCCCAGC GGCAGGCCGA GGCACTGGGC 
TACCAGGCTG TGGTGCTGAG TGCAGCCATG CAAGGTGATG TAAAAAGTAT GGCCCAGTTC 
TACGGGCTGC TGGCCCATGT GGCTAGAACC CGCCTCACCC CATCCATGGC TGGGGCTTCT 
GTGGAGGAAG ATGCACAGCT CCATGAGCTG GCAGCTGAGC TTCAGATCCC AGACCTGCAG 
CTGGAGGAGG CTCTGGAGAC CATGGCATGG GGAAGGGGCC CAGTCTGCCT GCTGGCTGGT 
GGCGAGCCCA CAGTACAGCT GCAGGGCTCG GGCAGGGGTG GCCGGAACCA GGAACTGGCC 
CTGCGTGTTG GAGCAGAGTT GAGAAGGTGG CCGCTGGGGC CGATAGATGT GCTGTTTTTG 
AGCGGTGGCA CCGATGGGCA GGATGGGCCC ACAGAGGCTG CTGGGGCCTG GGTCACACCT 
GAGCTTGCCA GCCAGGCTGC AGCTGAGGGC CTGGACATAG CCACCTTCCT AGCCCACAAT 
GACTCACATA CCTTCTTCTG CTGCCTCCAG GGTGGGGCAC ACCTGCTGCA CACAGGGATG 
ACAGGTACCA ATGTCATGGA CACCCACCTC TTGTTCCTGC GGCCTCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association analysis identifies 13 new risk loci for schizophrenia.
Nature genetics45(10)1150-9(2013 Oct)
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Large-scale genome-wide association analysis of bipolar disorder identifies a new susceptibility locus near ODZ4.
Nature genetics43(10)977-83(2011 Sep)

D-glyceric aciduria is caused by genetic deficiency of D-glycerate kinase (GLYCTK).
Human mutation31(12)1280-5(2010 Dec)
Sass JO,Fischer K,Wang R,Christensen E,Scholl-B?rgi S,Chang R,Kapelari K,Walter M

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Mutation research694(1-2)13-9(2010 Dec)
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An empirical framework for binary interactome mapping.
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GLYCTK cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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