CHRM4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHRM4
Entrez Gene ID	1132
Full Name	cholinergic receptor muscarinic 4
Synonyms	HM4,M4R
General protein information	Preferred Names cholinergic receptor muscarinic 4 Names muscarinic acetylcholine receptor M4 acetylcholine receptor, muscarinic 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p11.2
Summary	The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008].
Disorder MIM:	118495

mRNA and Protein(s)

mRNA	Protein	Name
NM_001366692.1	NP_001353621.1	muscarinic acetylcholine receptor M4
NM_000741.3	NP_000732.2	muscarinic acetylcholine receptor M4
NM_000741.4	NP_000732.2	muscarinic acetylcholine receptor M4
NM_000741.5	NP_000732.2	muscarinic acetylcholine receptor M4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHRM4	XP_005631350.1
Rattus norvegicus (Norway rat)	Chrm4	NP_113735.1
Gallus gallus (chicken)	CHRM4	NP_001026362.1
Homo sapiens (human)	CHRM4	NP_000732.2
Mus musculus (house mouse)	Chrm4	NP_031725.1
Danio rerio (zebrafish)	chrm4a	XP_001922442.1
	CHRM4	XP_001112370.1
Xenopus tropicalis (tropical clawed frog)	chrm4	NP_001106514.1

Gene Ontology
Bibliography

Related articles in PubMed

African-specific variability in the acetylcholine muscarinic receptor M4: association with cocaine and heroin addiction.
Levran O, Randesi M, Peles E, Correa da Rosa J, Ott J, Rotrosen J, Adelson M, Kreek MJ
Pharmacogenomics17(9)995-1003(2016 Jun)

Crystal structures of the M1 and M4 muscarinic acetylcholine receptors.
Thal DM, Sun B, Feng D, Nawaratne V, Leach K, Felder CC, Bures MG, Evans DA, Weis WI, Bachhawat P, Kobilka TS, Sexton PM, Kobilka BK, Christopoulos A
Nature531(7594)335-40(2016 Mar)

Modified expression of peripheral blood lymphocyte muscarinic cholinergic receptors in asthmatic children.
Cherubini E, Tabb? L, Scozzi D, Mariotta S, Galli E, Carello R, Avitabile S, Tayebati SK, Amenta F, De Vitis C, Mancini R, Ricci A
Journal of neuroimmunology28437-43(2015 Jul)

Development of a radioligand, [(3)H]LY2119620, to probe the human M(2) and M(4) muscarinic receptor allosteric binding sites.
Schober DA, Croy CH, Xiao H, Christopoulos A, Felder CC
Molecular pharmacology86(1)116-23(2014 Jul)

Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus?midbrain pathway for feeding behavior.
Stachniak TJ, Ghosh A, Sternson SM
Neuron82(4)797-808(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the association of the African-specific CHRM4 SNP with cocaine dependence survived correction for multiple testing
Title: African-specific variability in the acetylcholine muscarinic receptor M4: association with cocaine and heroin addiction.

crystal structures of the M1 and M4 muscarinic receptors bound to the inverse agonist, tiotropium
Title: Crystal structures of the M1 and M4 muscarinic acetylcholine receptors.

This study demonstrated that CD4+ and CD8+ T cells expressed M2 and M4 muscarinic receptors which density were significantly increased in asthmatic children in comparison with controls.
Title: Modified expression of peripheral blood lymphocyte muscarinic cholinergic receptors in asthmatic children.

[(3)H]LY2119620 to probe specifically the human M2 and M4 muscarinic receptor allosteric binding sites.
Title: Development of a radioligand, [(3)H]LY2119620, to probe the human M(2) and M(4) muscarinic receptor allosteric binding sites.

This study identify suppression of the PVHSIM1-->PAGvl/DR circuit connection as sufficient to induce overeating behavior.
Title: Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus?midbrain pathway for feeding behavior.

The following CHRM4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHRM4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10556	NM_000741.5 Latest version!	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu10556	NM_001366692.1 Latest version!	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu10556	NM_000741.3	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu10556	NM_000741.4	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHRM4 ( NM_001366692.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001353621.1 Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10556
Clone ID Related Accession (Same CDS sequence)	NM_001366692.1 , NM_000741.3 , NM_000741.4 , NM_000741.5
Accession Version	NM_001366692.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116021.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001353621.1
CDS	80..1519
Translation	MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	NM_001366692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001366692.1

ORF Insert Sequence:

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM4 ( NM_000741.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000732.2 Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), mRNA.

Price & Availability↑

CloneID	OHu10556
Clone ID Related Accession (Same CDS sequence)	NM_001366692.1 , NM_000741.3 , NM_000741.4 , NM_000741.5
Accession Version	NM_000741.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC137535.1 and BF724332.1. On Feb 7, 2015 this sequence version replaced NM_000741.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000732.2
CDS	52..1491
Translation	MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	NM_000741.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000741.3

ORF Insert Sequence:

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM4 ( NM_000741.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000732.2 Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10556
Clone ID Related Accession (Same CDS sequence)	NM_001366692.1 , NM_000741.3 , NM_000741.4 , NM_000741.5
Accession Version	NM_000741.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116021.5. On Oct 16, 2018 this sequence version replaced NM_000741.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2155751 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000732.2
CDS	114..1553
Translation	MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	NM_000741.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000741.4

ORF Insert Sequence:

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHRM4 ( NM_000741.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000732.2 Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.

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CloneID	OHu10556
Clone ID Related Accession (Same CDS sequence)	NM_001366692.1 , NM_000741.3 , NM_000741.4 , NM_000741.5
Accession Version	NM_000741.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	muscarinic acetylcholine receptor M4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC116021.5. On Aug 27, 2019 this sequence version replaced NM_000741.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000732.2
CDS	276..1715
Translation	MANFTPVNGSSGNQSVRLVTSSSHNRYETVEMVFIATVTGSLSLVTVVGNILVMLSIKVNRQLQTVNNYFLFSLACADLIIGAFSMNLYTVYIIKGYWPLGAVVCDLWLALDYVVSNASVMNLLIISFDRYFCVTKPLTYPARRTTKMAGLMIAAAWVLSFVLWAPAILFWQFVVGKRTVPDNQCFIQFLSNPAVTFGTAIAAFYLPVVIMTVLYIHISLASRSRVHKHRPEGPKEKKAKTLAFLKSPLMKQSVKKPPPGEAAREELRNGKLEEAPPPALPPPPRPVADKDTSNESSSGSATQNTKERPATELSTTEATTPAMPAPPLQPRALNPASRWSKIQIVTKQTGNECVTAIEIVPATPAGMRPAANVARKFASIARNQVRKKRQMAARERKVTRTIFAILLAFILTWTPYNVMVLVNTFCQSCIPDTVWSIGYWLCYVNSTINPACYALCNATFKKTFRHLLLCQYRNIGTAR

Target ORF information:

RefSeq Version	NM_000741.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cholinergic receptor muscarinic 4 (CHRM4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000741.5

ORF Insert Sequence:

ATGGCCAACT TCACACCTGT CAATGGCAGC TCGGGCAATC AGTCCGTGCG CCTGGTCACG 
TCATCATCCC ACAATCGCTA TGAGACGGTG GAAATGGTCT TCATTGCCAC AGTGACAGGC 
TCCCTGAGCC TGGTGACTGT CGTGGGCAAC ATCCTGGTGA TGCTGTCCAT CAAGGTCAAC 
AGGCAGCTGC AGACAGTCAA CAACTACTTC CTCTTCAGCC TGGCGTGTGC TGATCTCATC 
ATAGGCGCCT TCTCCATGAA CCTCTACACC GTGTACATCA TCAAGGGCTA CTGGCCCCTG 
GGCGCCGTGG TCTGCGACCT GTGGCTGGCC CTGGACTACG TGGTGAGCAA CGCCTCCGTC 
ATGAACCTTC TCATCATCAG CTTTGACCGC TACTTCTGCG TCACCAAGCC TCTCACCTAC 
CCTGCCCGGC GCACCACCAA GATGGCAGGC CTCATGATTG CTGCTGCCTG GGTACTGTCC 
TTCGTGCTCT GGGCGCCTGC CATCTTGTTC TGGCAGTTTG TGGTGGGTAA GCGGACGGTG 
CCCGACAACC AGTGCTTCAT CCAGTTCCTG TCCAACCCAG CAGTGACCTT TGGCACAGCC 
ATTGCTGCCT TCTACCTGCC TGTGGTCATC ATGACGGTGC TGTACATCCA CATCTCCCTG 
GCCAGTCGCA GCCGAGTCCA CAAGCACCGG CCCGAGGGCC CGAAGGAGAA GAAAGCCAAG 
ACGCTGGCCT TCCTCAAGAG CCCACTAATG AAGCAGAGCG TCAAGAAGCC CCCGCCCGGG 
GAGGCCGCCC GGGAGGAGCT GCGCAATGGC AAGCTGGAGG AGGCCCCCCC GCCAGCGCTG 
CCACCGCCAC CGCGCCCCGT GGCTGATAAG GACACTTCCA ATGAGTCCAG CTCAGGCAGT 
GCCACCCAGA ACACCAAGGA ACGCCCAGCC ACAGAGCTGT CCACCACAGA GGCCACCACG 
CCCGCCATGC CCGCCCCTCC CCTGCAGCCG CGGGCCCTCA ACCCAGCCTC CAGATGGTCC 
AAGATCCAGA TTGTGACGAA GCAGACAGGC AATGAGTGTG TGACAGCCAT TGAGATTGTG 
CCTGCCACGC CGGCTGGCAT GCGCCCTGCG GCCAACGTGG CCCGCAAGTT CGCCAGCATC 
GCTCGCAACC AGGTGCGCAA GAAGCGGCAG ATGGCGGCCC GGGAGCGCAA AGTGACACGA 
ACGATCTTTG CCATTCTGCT AGCCTTCATC CTCACCTGGA CGCCCTACAA CGTCATGGTC 
CTGGTGAACA CCTTCTGCCA GAGCTGCATC CCTGACACGG TGTGGTCCAT TGGCTACTGG 
CTCTGCTACG TCAACAGCAC CATCAACCCT GCCTGCTATG CTCTGTGCAA CGCCACCTTT 
AAAAAGACCT TCCGGCACCT GCTGCTGTGC CAGTATCGGA ACATCGGCAC TGCCAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

African-specific variability in the acetylcholine muscarinic receptor M4: association with cocaine and heroin addiction.
Pharmacogenomics17(9)995-1003(2016 Jun)
Levran O,Randesi M,Peles E,Correa da Rosa J,Ott J,Rotrosen J,Adelson M,Kreek MJ

Crystal structures of the M1 and M4 muscarinic acetylcholine receptors.
Nature531(7594)335-40(2016 Mar)
Thal DM,Sun B,Feng D,Nawaratne V,Leach K,Felder CC,Bures MG,Evans DA,Weis WI,Bachhawat P,Kobilka TS,Sexton PM,Kobilka BK,Christopoulos A

Modified expression of peripheral blood lymphocyte muscarinic cholinergic receptors in asthmatic children.
Journal of neuroimmunology28437-43(2015 Jul)
Cherubini E,Tabb? L,Scozzi D,Mariotta S,Galli E,Carello R,Avitabile S,Tayebati SK,Amenta F,De Vitis C,Mancini R,Ricci A

Development of a radioligand, [(3)H]LY2119620, to probe the human M(2) and M(4) muscarinic receptor allosteric binding sites.
Molecular pharmacology86(1)116-23(2014 Jul)
Schober DA,Croy CH,Xiao H,Christopoulos A,Felder CC

Chemogenetic synaptic silencing of neural circuits localizes a hypothalamus?midbrain pathway for feeding behavior.
Neuron82(4)797-808(2014 May)
Stachniak TJ,Ghosh A,Sternson SM

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CHRM4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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