MAN1B1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN1B1
Entrez Gene ID	11253
Full Name	mannosidase alpha class 1B member 1
Synonyms	ERMAN1,ERManI,MANA-ER,MRT15
General protein information	Preferred Names mannosidase alpha class 1B member 1 Names endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase ER alpha 1,2-mannosidase Man9GlcNAc2-specific processing alpha-mannosidase endoplasmic Reticulum Class I alpha-mannosidase endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.3
Summary	This gene encodes an enzyme belonging to the glycosyl hydrolase 47 family. This enzyme functions in N-glycan biosynthesis, and is a class I alpha-1,2-mannosidase that specifically converts Man9GlcNAc to Man8GlcNAc isomer B. It is required for N-glycan trimming to Man5-6GlcNAc2 in the endoplasmic-reticulum-associated degradation pathway. Mutations in this gene cause autosomal-recessive intellectual disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Dec 2011].
Disorder MIM:	604346
Disorder Html:	Mental retardation, autosomal recessive 15, 614202 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006716945.4	XP_006717008.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X2
NM_016219.4	NP_057303.2	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
XM_017014239.1	XP_016869728.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X3
XM_024447403.1	XP_024303171.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
NM_016219.5	NP_057303.2	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
XM_006716945.3	XP_006717008.1	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Man1b1	NP_001025154.1
Danio rerio (zebrafish)	si:ch211-181b18.1	XP_005161176.1
Caenorhabditis elegans (roundworm)	T03G11.4	NP_508877.1
Caenorhabditis elegans (roundworm)	ZC410.3	NP_001255362.1
Oryza sativa (rice)	Os05g0209100	NP_001054909.1
Xenopus tropicalis (tropical clawed frog)	man1b1	NP_001120156.1
Homo sapiens (human)	MAN1B1	NP_057303.2
Pan troglodytes (chimpanzee)	MAN1B1	XP_001135997.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003884	XP_003436647.1
Bos taurus (cattle)	MAN1B1	XP_002691756.3
	MAN1B1	XP_001091256.1
Canis lupus familiaris (dog)	MAN1B1	XP_537786.2
Gallus gallus (chicken)	MAN1B1	XP_415569.4
Drosophila melanogaster (fruit fly)	alpha-Man-Ib	NP_651667.1
Arabidopsis thaliana (thale cress)	MNS3	NP_564345.1
Rattus norvegicus (Norway rat)	Man1b1	NP_001102666.1
Eremothecium gossypii	AGOS_AER165W	NP_985023.1

Gene Ontology
Bibliography

Related articles in PubMed

Single-center experience of N-linked Congenital Disorders of Glycosylation with a Summary of Molecularly Characterized Cases in Arabs.
Bastaki F, Bizzari S, Hamici S, Nair P, Mohamed M, Saif F, Malik EM, Al-Ali MT, Hamzeh AR
Annals of human genetics82(1)35-47(2018 Jan)

ERManI (Endoplasmic Reticulum Class I ?-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway.
Zhou T, Frabutt DA, Moremen KW, Zheng YH
The Journal of biological chemistry290(36)22184-92(2015 Sep)

Mammalian ER mannosidase I resides in quality control vesicles, where it encounters its glycoprotein substrates.
Benyair R, Ogen-Shtern N, Mazkereth N, Shai B, Ehrlich M, Lederkremer GZ
Molecular biology of the cell26(2)172-84(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that HIV-1 env protein interacts with alpha-mannosidases ERManI (MAN1B1) and initiates endoplasmic reticulum (ER)-associated protein degradation (ERAD) pathway degradation process.
Title: ERManI (Endoplasmic Reticulum Class I ?-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway.

Results suggest that endoplasmic reticulum mannosidase I (ERManI) is turned over by an active autophagic process.
Title: Mammalian ER mannosidase I resides in quality control vesicles, where it encounters its glycoprotein substrates.

The properties of ERMAN1 enable rapid selection of endoplasmic reticulum-associated degradation substrates in the endoplasmic reticulum.ERMAN1 digests mono-, di- and tri-glucosylated N-glycans.
Title: Trimming of glucosylated N-glycans by human ER ?1,2-mannosidase I.

MAN1B1 deficiency appeared to be a frequent cause in our cohort of patients with unsolved congenital disorder of glycosylation type II.
Title: Diagnostic serum glycosylation profile in patients with intellectual disability as a result of MAN1B1 deficiency.

we linked mutations in MAN1B1 to a Golgi glycosylation disorder. Additionally, our results support the recent findings on MAN1B1 localization
Title: MAN1B1 deficiency: an unexpected CDG-II.

The following MAN1B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN1B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26744	NM_016219.5 Latest version!	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu42955	XM_006716945.4 Latest version!	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu106099	XM_024447403.1 Latest version!	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu98421	XM_017014239.1 Latest version!	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu26744	NM_016219.4	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu42955	XM_006716945.3	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN1B1 ( NM_016219.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_057303.2 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26744
Clone ID Related Accession (Same CDS sequence)	NM_016219.4 , NM_016219.5
Accession Version	NM_016219.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006079.1 and BC002953.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_016219.4. Transcript Variant: This variant (1) encodes the supported protein. Sequence Note: This RefSeq uses the 5'-most in-frame start codon, resulting in a protein N-terminus that is primate-specific. The use of an alternative downstream start codon, which is better conserved among vertebrates, is also possible, and would result in a protein that is 36 aa shorter at the N-terminus. It is unknown which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006079.1, AY358465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000371589.9/ ENSP00000360645.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTCC CCTCGGGTGG CTATTCTTCC 
ATCAACAATG TCCAGGATCC TCAGAAGCCC GAGCCTAGGG ACAAGATGGA GAGCTTCTTC 
CTGGGGGAGA CGCTCAAGTA TCTGTTCTTG CTCTTCTCCG ATGACCCAAA CCTGCTCAGC 
CTGGATGCCT ACGTGTTCAA CACCGAAGCC CACCCTCTGC CTATCTGGAC CCCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057303.2
CDS	16..2115
Translation	MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA

Target ORF information:

RefSeq Version	NM_016219.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016219.5

ORF Insert Sequence:

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTCC CCTCGGGTGG CTATTCTTCC 
ATCAACAATG TCCAGGATCC TCAGAAGCCC GAGCCTAGGG ACAAGATGGA GAGCTTCTTC 
CTGGGGGAGA CGCTCAAGTA TCTGTTCTTG CTCTTCTCCG ATGACCCAAA CCTGCTCAGC 
CTGGATGCCT ACGTGTTCAA CACCGAAGCC CACCCTCTGC CTATCTGGAC CCCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1B1 ( NM_016219.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_057303.2 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26744
Clone ID Related Accession (Same CDS sequence)	NM_016219.4 , NM_016219.5
Accession Version	NM_016219.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-14
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM848429.1, AF148509.1, BC006079.1, BC002953.1 and BQ448144.1. This sequence is a reference standard in the RefSeqGene project. On Dec 28, 2011 this sequence version replaced NM_016219.3. Transcript Variant: This variant (1) encodes the supported protein. Sequence Note: This RefSeq uses the 5'-most in-frame start codon, resulting in a protein N-terminus that is primate-specific. The use of an alternative downstream start codon, which is better conserved among vertebrates, is also possible, and would result in a protein that is 36 aa shorter at the N-terminus. It is unknown which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY358465.1, SRR1803616.74773.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTCC CCTCGGGTGG CTATTCTTCC 
ATCAACAATG TCCAGGATCC TCAGAAGCCC GAGCCTAGGG ACAAGATGGA GAGCTTCTTC 
CTGGGGGAGA CGCTCAAGTA TCTGTTCTTG CTCTTCTCCG ATGACCCAAA CCTGCTCAGC 
CTGGATGCCT ACGTGTTCAA CACCGAAGCC CACCCTCTGC CTATCTGGAC CCCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057303.2
CDS	74..2173
Translation	MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINNVQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPIWTPA

Target ORF information:

RefSeq Version	NM_016219.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016219.4

ORF Insert Sequence:

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTCC CCTCGGGTGG CTATTCTTCC 
ATCAACAATG TCCAGGATCC TCAGAAGCCC GAGCCTAGGG ACAAGATGGA GAGCTTCTTC 
CTGGGGGAGA CGCTCAAGTA TCTGTTCTTG CTCTTCTCCG ATGACCCAAA CCTGCTCAGC 
CTGGATGCCT ACGTGTTCAA CACCGAAGCC CACCCTCTGC CTATCTGGAC CCCTGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1B1 ( XM_006716945.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717008.1 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X2, mRNA.

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CloneID	OHu42955
Clone ID Related Accession (Same CDS sequence)	XM_006716945.4 , XM_006716945.3
Accession Version	XM_006716945.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006716945.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTGA GCACCTGTCC TCGCCCCGCG 
TGGTCACGGC CACCGGGCCA CAGGCACGGC TGGGCTGTGG GGCTCAGGCT GGCTCCGCTC 
TTGGTGGTGG CTGTGACCTG GATCCGGGAG GGGCGGGCTT GCCGCAGCCT CGGGGTGGCC 
ACACTGCAGC TTGGGGGCCC TGGCATCCCC ATCCCCACTG AGCTTCATGG TTGTGGGACC 
CAGAGTACTT GGAGGGGCTG GGCACCCCTC ATTTTCATTT CTCAGGGCCT GTCTAGGGAG 
GGTCTCTGCT GTGGGCCCAG CATCCCCCCA GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717008.1
CDS	36..2231
Translation	MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVSTCPRPAWSRPPGHRHGWAVGLRLAPLLVVAVTWIREGRACRSLGVATLQLGGPGIPIPTELHGCGTQSTWRGWAPLIFISQGLSREGLCCGPSIPPV

Target ORF information:

RefSeq Version	XM_006716945.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716945.4

ORF Insert Sequence:

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTGA GCACCTGTCC TCGCCCCGCG 
TGGTCACGGC CACCGGGCCA CAGGCACGGC TGGGCTGTGG GGCTCAGGCT GGCTCCGCTC 
TTGGTGGTGG CTGTGACCTG GATCCGGGAG GGGCGGGCTT GCCGCAGCCT CGGGGTGGCC 
ACACTGCAGC TTGGGGGCCC TGGCATCCCC ATCCCCACTG AGCTTCATGG TTGTGGGACC 
CAGAGTACTT GGAGGGGCTG GGCACCCCTC ATTTTCATTT CTCAGGGCCT GTCTAGGGAG 
GGTCTCTGCT GTGGGCCCAG CATCCCCCCA GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1B1 ( XM_006716945.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006717008.1 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.

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CloneID	OHu42955
Clone ID Related Accession (Same CDS sequence)	XM_006716945.4 , XM_006716945.3
Accession Version	XM_006716945.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716945.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTGA GCACCTGTCC TCGCCCCGCG 
TGGTCACGGC CACCGGGCCA CAGGCACGGC TGGGCTGTGG GGCTCAGGCT GGCTCCGCTC 
TTGGTGGTGG CTGTGACCTG GATCCGGGAG GGGCGGGCTT GCCGCAGCCT CGGGGTGGCC 
ACACTGCAGC TTGGGGGCCC TGGCATCCCC ATCCCCACTG AGCTTCATGG TTGTGGGACC 
CAGAGTACTT GGAGGGGCTG GGCACCCCTC ATTTTCATTT CTCAGGGCCT GTCTAGGGAG 
GGTCTCTGCT GTGGGCCCAG CATCCCCCCA GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006717008.1
CDS	139..2334
Translation	MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVSTCPRPAWSRPPGHRHGWAVGLRLAPLLVVAVTWIREGRACRSLGVATLQLGGPGIPIPTELHGCGTQSTWRGWAPLIFISQGLSREGLCCGPSIPPV

Target ORF information:

RefSeq Version	XM_006716945.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716945.3

ORF Insert Sequence:

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGCCAGCA GACAGGCACA ACCTGCTGCG GCCAGAGACC 
GTGGAGAGCC TGTTCTACCT GTACCGCGTC ACAGGGGACC GCAAATACCA GGACTGGGGC 
TGGGAGATTC TGCAGAGCTT CAGCCGATTC ACACGGGTGA GCACCTGTCC TCGCCCCGCG 
TGGTCACGGC CACCGGGCCA CAGGCACGGC TGGGCTGTGG GGCTCAGGCT GGCTCCGCTC 
TTGGTGGTGG CTGTGACCTG GATCCGGGAG GGGCGGGCTT GCCGCAGCCT CGGGGTGGCC 
ACACTGCAGC TTGGGGGCCC TGGCATCCCC ATCCCCACTG AGCTTCATGG TTGTGGGACC 
CAGAGTACTT GGAGGGGCTG GGCACCCCTC ATTTTCATTT CTCAGGGCCT GTCTAGGGAG 
GGTCTCTGCT GTGGGCCCAG CATCCCCCCA GTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1B1 ( XM_024447403.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303171.1 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu106099
Clone ID Related Accession (Same CDS sequence)	XM_024447403.1
Accession Version	XM_024447403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGGTGGGC CTGGGCCTGG GTCAGGGTCC ATCAGGAGGA 
GGGTGCTGGC AGGGCTGGCC TTGCCCTGAG CTCTGCTCCG CCCACAGCCA GCAGACAGGC 
ACAACCTGCT GCGGCCAGAG ACCGTGGAGA GCCTGTTCTA CCTGTACCGC GTCACAGGGG 
ACCGCAAATA CCAGGACTGG GGCTGGGAGA TTCTGCAGAG CTTCAGCCGA TTCACACGGG 
TCCCCTCGGG TGGCTATTCT TCCATCAACA ATGTCCAGGA TCCTCAGAAG CCCGAGCCTA 
GGGACAAGAT GGAGAGCTTC TTCCTGGGGG AGACGCTCAA GTATCTGTTC TTGCTCTTCT 
CCGATGACCC AAACCTGCTC AGCCTGGATG CCTACGTGTT CAACACCGAA GCCCACCCTC 
TGCCTATCTG GACCCCTGCC TAGGGTGGAT GGCTGCTGGT GTGGGGACTT CGGGTGGGCA 
GAGGCACCTT GCTGGGTCTG TGGCATTTTC CAAGGGCCCA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303171.1
CDS	36..2300
Translation	MAACEGRRSGALGSSQSDFLTPPVGGAPWAVATTVVMYPPPPPPPHRDFISVTLSFGENYDNSKSWRRRSCWRKWKQLSRLQRNMILFLLAFLLFCGLLFYINLADHWKALAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKVGLGLGQGPSGGGCWQGWPCPELCSAHSQQTGTTCCGQRPWRACSTCTASQGTANTRTGAGRFCRASADSHGSPRVAILPSTMSRILRSPSLGTRWRASSWGRRSSICSCSSPMTQTCSAWMPTCSTPKPTLCLSGPLPRVDGCWCGDFGWAEAPCWVCGIFQGPT

Target ORF information:

RefSeq Version	XM_024447403.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447403.1

ORF Insert Sequence:

ATGGCTGCCT GCGAGGGCAG GAGAAGCGGA GCTCTCGGTT CCTCTCAGTC GGACTTCCTG 
ACGCCGCCAG TGGGCGGGGC CCCTTGGGCC GTCGCCACCA CTGTAGTCAT GTACCCACCG 
CCGCCGCCGC CGCCTCATCG GGACTTCATC TCGGTGACGC TGAGCTTTGG CGAGAACTAT 
GACAACAGCA AGAGTTGGCG GCGGCGCTCG TGCTGGAGGA AATGGAAGCA ACTGTCGAGA 
TTGCAGCGGA ATATGATTCT CTTCCTCCTT GCCTTTCTGC TTTTCTGTGG ACTCCTCTTC 
TACATCAACT TGGCTGACCA TTGGAAAGCT CTGGCTTTCA GGCTAGAGGA AGAGCAGAAG 
ATGAGGCCAG AAATTGCTGG GTTAAAACCA GCAAATCCAC CCGTCTTACC AGCTCCTCAG 
AAGGCGGACA CCGACCCTGA GAACTTACCT GAGATTTCGT CACAGAAGAC ACAAAGACAC 
ATCCAGCGGG GACCACCTCA CCTGCAGATT AGACCCCCAA GCCAAGACCT GAAGGATGGG 
ACCCAGGAGG AGGCCACAAA AAGGCAAGAA GCCCCTGTGG ATCCCCGCCC GGAAGGAGAT 
CCGCAGAGGA CAGTCATCAG CTGGAGGGGA GCGGTGATCG AGCCTGAGCA GGGCACCGAG 
CTCCCTTCAA GAAGAGCAGA AGTGCCCACC AAGCCTCCCC TGCCACCGGC CAGGACACAG 
GGCACACCAG TGCATCTGAA CTATCGCCAG AAGGGCGTGA TTGACGTCTT CCTGCATGCA 
TGGAAAGGAT ACCGCAAGTT TGCATGGGGC CATGACGAGC TGAAGCCTGT GTCCAGGTCC 
TTCAGTGAGT GGTTTGGCCT CGGTCTCACA CTGATCGACG CGCTGGACAC CATGTGGATC 
TTGGGTCTGA GGAAAGAATT TGAGGAAGCC AGGAAGTGGG TGTCGAAGAA GTTACACTTT 
GAAAAGGACG TGGACGTCAA CCTGTTTGAG AGCACGATCC GCATCCTGGG GGGGCTCCTG 
AGTGCCTACC ACCTGTCTGG GGACAGCCTC TTCCTGAGGA AAGCTGAGGA TTTTGGAAAT 
CGGCTAATGC CTGCCTTCAG AACACCATCC AAGATTCCTT ACTCGGATGT GAACATCGGT 
ACTGGAGTTG CCCACCCGCC ACGGTGGACC TCCGACAGCA CTGTGGCCGA GGTGACCAGC 
ATTCAGCTGG AGTTCCGGGA GCTCTCCCGT CTCACAGGGG ATAAGAAGTT TCAGGAGGCA 
GTGGAGAAGG TGACACAGCA CATCCACGGC CTGTCTGGGA AGAAGGATGG GCTGGTGCCC 
ATGTTCATCA ATACCCACAG TGGCCTCTTC ACCCACCTGG GCGTATTCAC GCTGGGCGCC 
AGGGCCGACA GCTACTATGA GTACCTGCTG AAGCAGTGGA TCCAGGGCGG GAAGCAGGAG 
ACACAGCTGC TGGAAGACTA CGTGGAAGCC ATCGAGGGTG TCAGAACGCA CCTGCTGCGG 
CACTCCGAGC CCAGTAAGCT CACCTTTGTG GGGGAGCTTG CCCACGGCCG CTTCAGTGCC 
AAGATGGACC ACCTGGTGTG CTTCCTGCCA GGGACGCTGG CTCTGGGCGT CTACCACGGC 
CTGCCCGCCA GCCACATGGA GCTGGCCCAG GAGCTCATGG AGACTTGTTA CCAGATGAAC 
CGGCAGATGG AGACGGGGCT GAGTCCCGAG ATCGTGCACT TCAACCTTTA CCCCCAGCCG 
GGCCGTCGGG ACGTGGAGGT CAAGGTGGGC CTGGGCCTGG GTCAGGGTCC ATCAGGAGGA 
GGGTGCTGGC AGGGCTGGCC TTGCCCTGAG CTCTGCTCCG CCCACAGCCA GCAGACAGGC 
ACAACCTGCT GCGGCCAGAG ACCGTGGAGA GCCTGTTCTA CCTGTACCGC GTCACAGGGG 
ACCGCAAATA CCAGGACTGG GGCTGGGAGA TTCTGCAGAG CTTCAGCCGA TTCACACGGG 
TCCCCTCGGG TGGCTATTCT TCCATCAACA ATGTCCAGGA TCCTCAGAAG CCCGAGCCTA 
GGGACAAGAT GGAGAGCTTC TTCCTGGGGG AGACGCTCAA GTATCTGTTC TTGCTCTTCT 
CCGATGACCC AAACCTGCTC AGCCTGGATG CCTACGTGTT CAACACCGAA GCCCACCCTC 
TGCCTATCTG GACCCCTGCC TAGGGTGGAT GGCTGCTGGT GTGGGGACTT CGGGTGGGCA 
GAGGCACCTT GCTGGGTCTG TGGCATTTTC CAAGGGCCCA CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN1B1 ( XM_017014239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869728.1 Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X3, mRNA.

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CloneID	OHu98421
Clone ID Related Accession (Same CDS sequence)	XM_017014239.1
Accession Version	XM_017014239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1899bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTCTG ACTACTTTCT AGGTCTCTGG TCTCTGGCTT TCAGGCTAGA GGAAGAGCAG 
AAGATGAGGC CAGAAATTGC TGGGTTAAAA CCAGCAAATC CACCCGTCTT ACCAGCTCCT 
CAGAAGGCGG ACACCGACCC TGAGAACTTA CCTGAGATTT CGTCACAGAA GACACAAAGA 
CACATCCAGC GGGGACCACC TCACCTGCAG ATTAGACCCC CAAGCCAAGA CCTGAAGGAT 
GGGACCCAGG AGGAGGCCAC AAAAAGGCAA GAAGCCCCTG TGGATCCCCG CCCGGAAGGA 
GATCCGCAGA GGACAGTCAT CAGCTGGAGG GGAGCGGTGA TCGAGCCTGA GCAGGGCACC 
GAGCTCCCTT CAAGAAGAGC AGAAGTGCCC ACCAAGCCTC CCCTGCCACC GGCCAGGACA 
CAGGGCACAC CAGTGCATCT GAACTATCGC CAGAAGGGCG TGATTGACGT CTTCCTGCAT 
GCATGGAAAG GATACCGCAA GTTTGCATGG GGCCATGACG AGCTGAAGCC TGTGTCCAGG 
TCCTTCAGTG AGTGGTTTGG CCTCGGTCTC ACACTGATCG ACGCGCTGGA CACCATGTGG 
ATCTTGGGTC TGAGGAAAGA ATTTGAGGAA GCCAGGAAGT GGGTGTCGAA GAAGTTACAC 
TTTGAAAAGG ACGTGGACGT CAACCTGTTT GAGAGCACGA TCCGCATCCT GGGGGGGCTC 
CTGAGTGCCT ACCACCTGTC TGGGGACAGC CTCTTCCTGA GGAAAGCTGA GGATTTTGGA 
AATCGGCTAA TGCCTGCCTT CAGAACACCA TCCAAGATTC CTTACTCGGA TGTGAACATC 
GGTACTGGAG TTGCCCACCC GCCACGGTGG ACCTCCGACA GCACTGTGGC CGAGGTGACC 
AGCATTCAGC TGGAGTTCCG GGAGCTCTCC CGTCTCACAG GGGATAAGAA GTTTCAGGAG 
GCAGTGGAGA AGGTGACACA GCACATCCAC GGCCTGTCTG GGAAGAAGGA TGGGCTGGTG 
CCCATGTTCA TCAATACCCA CAGTGGCCTC TTCACCCACC TGGGCGTATT CACGCTGGGC 
GCCAGGGCCG ACAGCTACTA TGAGTACCTG CTGAAGCAGT GGATCCAGGG CGGGAAGCAG 
GAGACACAGC TGCTGGAAGA CTACGTGGAA GCCATCGAGG GTGTCAGAAC GCACCTGCTG 
CGGCACTCCG AGCCCAGTAA GCTCACCTTT GTGGGGGAGC TTGCCCACGG CCGCTTCAGT 
GCCAAGATGG ACCACCTGGT GTGCTTCCTG CCAGGGACGC TGGCTCTGGG CGTCTACCAC 
GGCCTGCCCG CCAGCCACAT GGAGCTGGCC CAGGAGCTCA TGGAGACTTG TTACCAGATG 
AACCGGCAGA TGGAGACGGG GCTGAGTCCC GAGATCGTGC ACTTCAACCT TTACCCCCAG 
CCGGGCCGTC GGGACGTGGA GGTCAAGCCA GCAGACAGGC ACAACCTGCT GCGGCCAGAG 
ACCGTGGAGA GCCTGTTCTA CCTGTACCGC GTCACAGGGG ACCGCAAATA CCAGGACTGG 
GGCTGGGAGA TTCTGCAGAG CTTCAGCCGA TTCACACGGG TGAGCACCTG TCCTCGCCCC 
GCGTGGTCAC GGCCACCGGG CCACAGGCAC GGCTGGGCTG TGGGGCTCAG GCTGGCTCCG 
CTCTTGGTGG TGGCTGTGAC CTGGATCCGG GAGGGGCGGG CTTGCCGCAG CCTCGGGGTG 
GCCACACTGC AGCTTGGGGG CCCTGGCATC CCCATCCCCA CTGAGCTTCA TGGTTGTGGG 
ACCCAGAGTA CTTGGAGGGG CTGGGCACCC CTCATTTTCA TTTCTCAGGG CCTGTCTAGG 
GAGGGTCTCT GCTGTGGGCC CAGCATCCCC CCAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869728.1
CDS	348..2246
Translation	MLSDYFLGLWSLAFRLEEEQKMRPEIAGLKPANPPVLPAPQKADTDPENLPEISSQKTQRHIQRGPPHLQIRPPSQDLKDGTQEEATKRQEAPVDPRPEGDPQRTVISWRGAVIEPEQGTELPSRRAEVPTKPPLPPARTQGTPVHLNYRQKGVIDVFLHAWKGYRKFAWGHDELKPVSRSFSEWFGLGLTLIDALDTMWILGLRKEFEEARKWVSKKLHFEKDVDVNLFESTIRILGGLLSAYHLSGDSLFLRKAEDFGNRLMPAFRTPSKIPYSDVNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKDGLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQGGKQETQLLEDYVEAIEGVRTHLLRHSEPSKLTFVGELAHGRFSAKMDHLVCFLPGTLALGVYHGLPASHMELAQELMETCYQMNRQMETGLSPEIVHFNLYPQPGRRDVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVSTCPRPAWSRPPGHRHGWAVGLRLAPLLVVAVTWIREGRACRSLGVATLQLGGPGIPIPTELHGCGTQSTWRGWAPLIFISQGLSREGLCCGPSIPPV

Target ORF information:

RefSeq Version	XM_017014239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 1B member 1 (MAN1B1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014239.1

ORF Insert Sequence:

ATGCTGTCTG ACTACTTTCT AGGTCTCTGG TCTCTGGCTT TCAGGCTAGA GGAAGAGCAG 
AAGATGAGGC CAGAAATTGC TGGGTTAAAA CCAGCAAATC CACCCGTCTT ACCAGCTCCT 
CAGAAGGCGG ACACCGACCC TGAGAACTTA CCTGAGATTT CGTCACAGAA GACACAAAGA 
CACATCCAGC GGGGACCACC TCACCTGCAG ATTAGACCCC CAAGCCAAGA CCTGAAGGAT 
GGGACCCAGG AGGAGGCCAC AAAAAGGCAA GAAGCCCCTG TGGATCCCCG CCCGGAAGGA 
GATCCGCAGA GGACAGTCAT CAGCTGGAGG GGAGCGGTGA TCGAGCCTGA GCAGGGCACC 
GAGCTCCCTT CAAGAAGAGC AGAAGTGCCC ACCAAGCCTC CCCTGCCACC GGCCAGGACA 
CAGGGCACAC CAGTGCATCT GAACTATCGC CAGAAGGGCG TGATTGACGT CTTCCTGCAT 
GCATGGAAAG GATACCGCAA GTTTGCATGG GGCCATGACG AGCTGAAGCC TGTGTCCAGG 
TCCTTCAGTG AGTGGTTTGG CCTCGGTCTC ACACTGATCG ACGCGCTGGA CACCATGTGG 
ATCTTGGGTC TGAGGAAAGA ATTTGAGGAA GCCAGGAAGT GGGTGTCGAA GAAGTTACAC 
TTTGAAAAGG ACGTGGACGT CAACCTGTTT GAGAGCACGA TCCGCATCCT GGGGGGGCTC 
CTGAGTGCCT ACCACCTGTC TGGGGACAGC CTCTTCCTGA GGAAAGCTGA GGATTTTGGA 
AATCGGCTAA TGCCTGCCTT CAGAACACCA TCCAAGATTC CTTACTCGGA TGTGAACATC 
GGTACTGGAG TTGCCCACCC GCCACGGTGG ACCTCCGACA GCACTGTGGC CGAGGTGACC 
AGCATTCAGC TGGAGTTCCG GGAGCTCTCC CGTCTCACAG GGGATAAGAA GTTTCAGGAG 
GCAGTGGAGA AGGTGACACA GCACATCCAC GGCCTGTCTG GGAAGAAGGA TGGGCTGGTG 
CCCATGTTCA TCAATACCCA CAGTGGCCTC TTCACCCACC TGGGCGTATT CACGCTGGGC 
GCCAGGGCCG ACAGCTACTA TGAGTACCTG CTGAAGCAGT GGATCCAGGG CGGGAAGCAG 
GAGACACAGC TGCTGGAAGA CTACGTGGAA GCCATCGAGG GTGTCAGAAC GCACCTGCTG 
CGGCACTCCG AGCCCAGTAA GCTCACCTTT GTGGGGGAGC TTGCCCACGG CCGCTTCAGT 
GCCAAGATGG ACCACCTGGT GTGCTTCCTG CCAGGGACGC TGGCTCTGGG CGTCTACCAC 
GGCCTGCCCG CCAGCCACAT GGAGCTGGCC CAGGAGCTCA TGGAGACTTG TTACCAGATG 
AACCGGCAGA TGGAGACGGG GCTGAGTCCC GAGATCGTGC ACTTCAACCT TTACCCCCAG 
CCGGGCCGTC GGGACGTGGA GGTCAAGCCA GCAGACAGGC ACAACCTGCT GCGGCCAGAG 
ACCGTGGAGA GCCTGTTCTA CCTGTACCGC GTCACAGGGG ACCGCAAATA CCAGGACTGG 
GGCTGGGAGA TTCTGCAGAG CTTCAGCCGA TTCACACGGG TGAGCACCTG TCCTCGCCCC 
GCGTGGTCAC GGCCACCGGG CCACAGGCAC GGCTGGGCTG TGGGGCTCAG GCTGGCTCCG 
CTCTTGGTGG TGGCTGTGAC CTGGATCCGG GAGGGGCGGG CTTGCCGCAG CCTCGGGGTG 
GCCACACTGC AGCTTGGGGG CCCTGGCATC CCCATCCCCA CTGAGCTTCA TGGTTGTGGG 
ACCCAGAGTA CTTGGAGGGG CTGGGCACCC CTCATTTTCA TTTCTCAGGG CCTGTCTAGG 
GAGGGTCTCT GCTGTGGGCC CAGCATCCCC CCAGTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single-center experience of N-linked Congenital Disorders of Glycosylation with a Summary of Molecularly Characterized Cases in Arabs.
Annals of human genetics82(1)35-47(2018 Jan)
Bastaki F,Bizzari S,Hamici S,Nair P,Mohamed M,Saif F,Malik EM,Al-Ali MT,Hamzeh AR

ERManI (Endoplasmic Reticulum Class I ?-Mannosidase) Is Required for HIV-1 Envelope Glycoprotein Degradation via Endoplasmic Reticulum-associated Protein Degradation Pathway.
The Journal of biological chemistry290(36)22184-92(2015 Sep)
Zhou T,Frabutt DA,Moremen KW,Zheng YH

Mammalian ER mannosidase I resides in quality control vesicles, where it encounters its glycoprotein substrates.
Molecular biology of the cell26(2)172-84(2015 Jan)
Benyair R,Ogen-Shtern N,Mazkereth N,Shai B,Ehrlich M,Lederkremer GZ

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MAN1B1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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