alpha-Man-Ib cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	alpha-Man-Ib
Entrez Gene ID	43436
Full Name	alpha-Mannosidase class I b
Synonyms	CG11874,Dmel\CG11874,ORE-11,dMas-3,mas-3
General protein information	Preferred Names alpha-Mannosidase class I b Names CG11874 gene product from transcript CG11874-RA CG11874-PA alpha mannosidase Ib alpha-Man-Ib-PA
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F10-98F10

mRNA and Protein(s)

mRNA	Protein	Name
NM_143410.4	NP_651667.1	alpha-Mannosidase class I b

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Man1b1	NP_001025154.1
Danio rerio (zebrafish)	si:ch211-181b18.1	XP_005161176.1
Caenorhabditis elegans (roundworm)	T03G11.4	NP_508877.1
Caenorhabditis elegans (roundworm)	ZC410.3	NP_001255362.1
Oryza sativa (rice)	Os05g0209100	NP_001054909.1
Xenopus tropicalis (tropical clawed frog)	man1b1	NP_001120156.1
Homo sapiens (human)	MAN1B1	NP_057303.2
Pan troglodytes (chimpanzee)	MAN1B1	XP_001135997.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003884	XP_003436647.1
Bos taurus (cattle)	MAN1B1	XP_002691756.3
	MAN1B1	XP_001091256.1
Canis lupus familiaris (dog)	MAN1B1	XP_537786.2
Gallus gallus (chicken)	MAN1B1	XP_415569.4
Drosophila melanogaster (fruit fly)	alpha-Man-Ib	NP_651667.1
Arabidopsis thaliana (thale cress)	MNS3	NP_564345.1
Rattus norvegicus (Norway rat)	Man1b1	NP_001102666.1
Eremothecium gossypii	AGOS_AER165W	NP_985023.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following alpha-Man-Ib gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the alpha-Man-Ib cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa06456	NM_143410.4 Latest version!	Drosophila melanogaster alpha-Mannosidase class I b (alpha-Man-Ib), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

alpha-Man-Ib ( NM_143410.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651667.1 Drosophila melanogaster alpha-Mannosidase class I b (alpha-Man-Ib), mRNA.

Price & Availability↑

CloneID	OFa06456
Clone ID Related Accession (Same CDS sequence)	NM_143410.4
Accession Version	NM_143410.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	alpha-Mannosidase class I b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_143410.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGGACG GCGGTCACAT AACGCTCACA CTCCCGCCGG GATCAGCGGC GACGACGGAC 
GAGCGGGGCA GGAAGAGCCT GCGTCGGGCT TGGAATCAGC TACCGCGTTG TCAGCGCAAT 
CTCATTATTC TGGGCATCAC CGGATTCTGC GTGACAGTGT TGCTCTGCCT AAGTGGCGAT 
CAGCTGCTGG CTGCCAGCTT AAAGGATGTG GACACCAAGG CCGAGGTGGC AGCATATCAA 
ATGCCCCTGC CCAATCCGCA CCATCATCCG GGGGTGGATA AGGATGTGCC CAAAGACACT 
GCTGCGGTAC CTGTTCCGGC CGATAAGAGA GCACCCCGGG TGGAGGCGGT TAGTGATAAG 
TTTAATGAGC TAGTTGCCTA TGGAGAAGCG AACTCAGCGG AGAACGTGGC CCGTCTGCCG 
GAGACGGTGG CTGCTGAAGG ACTTCCCCAG GCCTCGGCCA TGGCCGTGGC CTTGCCTCCC 
CCCGATAGTA ACAAGGAGCA GGACATCGGT GGAATCGACA TCCTAAACAA TGTAGAGGAG 
CCCGTCGCCC AGGATCCAGC AGAGCCGCTT AGCGAGGGCG AGCAGCTTGA GCAAGCCATC 
AAGAACCCAC TCACTCTGGG CGGCGTTAGC TTCAAAGAGC ATCTCAAAAA GATCAAGCCC 
ATTCTGGCCA AGGGCCAGCA CTTCCATGGC GCCACCAACG AAAGACAGTC GGCTGTTGTA 
GCCGCCTTCA AGCACTCCTG GGCTGGCTAC AAGAAGTATG CCTGGGGCCA CGACAACCTG 
AAGCCCATCT CCCAGTACTC CCACGAGTGG TTCGGTCTGG GACTAACCAT TGTTGACTCA 
TTGGATACCA TGTACATAAT GGGACTGGAT GATGAATTCA AGGAAGGACG CGACTGGGTA 
GAGCAATCAC TGCGCTTTGA TACCAAGCGC GATGTAAACC TATTCGAGGT CACCATACGT 
GTCCTAGGCG GACTGCTGTC TGCATATCAC CTGAGCGGCG ATACAATGTT TCTGGCCAAG 
GCTGCGGAAC TTGGCAACCG TTTACTTCCC GCCTTTCAAT CGCCGTCAAA TATCCCCTAT 
TCTGATGTGA ATCTCGGAGA TCTTTCGGCG CACTCGCCGA AGTGGTCTCC TGATAGCTCC 
ACCAGTGAGG TCACCACCAT TCAGCTAGAG TTTCGTGATT TGAGTCGCTC TACAAACATC 
TCCATTTATG AACAGGTTGC TCACAAAGTA AACGAGAAGG TGCATGATCT GGAGAAGAAC 
CATGGTTTGG TGCCCATATT CATTAACGCC AACACAGGAA CATTCCGAAA CTACGCCACT 
ATCTCGCTTG GTGCTCGTGG GGACTCCTAC TATGAGTACT TGCTCAAACA GTGGATTCAG 
ACCGGCCGCA AGGATAATGA TAACCTTATT TTGGACTACA TGCAAGCAGT CGATGGTGTT 
CTAACACAAC TGATGCGGCG CACGCCACGC GAGCACTGGG TTTACATTGG CGAGCTGATC 
AATGGCAAAG ACTTTAAGCC GAAAATGGAT CACCTTACGT GTTATCTGCC GGGCACTTTG 
ATTCTGGGTC ACCAGAACGG AATGCCAGAC TCGCACCTTA TACTTGCGAG GGATTTATTG 
GACACATGCT ATCAGACCTA TATGATGAAT CCCACACATT TGGCTGCTGA GATCTCGTAT 
TTTGCGCTAA CCGAAAAGGA CGATCAGGAC ATTTATGTGA AGCCAAACGA TGCCCACAAC 
CTGTTGCGCC CGGAGTTTGT GGAGAGTCTC TATTACTTCT ACTCAATAAC TGGCAATCGG 
ACATACCAGG ACATGGGATG GAAAATCTTC CAGGCGTTTG AGACTCACGC TAAAGTAAAT 
GCAGGCTACA CATCGATGGG AAATGTAAAG AACACACAGA GCACGCGTCT GCGCGATCTG 
ATGGAAAGCT TTTGGATGAG CGAGACCCTG AAGTACTTCT ACCTGCTGTT TAGCGACGAC 
CGCAAGGAAA TCGACCTGGA GCAATGGGTC TTCAATTCGG AGGGCCATCC ACTGCCGGTC 
TATCCGCTCA AGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651667.1
CDS	326..2383
Translation	MKDGGHITLTLPPGSAATTDERGRKSLRRAWNQLPRCQRNLIILGITGFCVTVLLCLSGDQLLAASLKDVDTKAEVAAYQMPLPNPHHHPGVDKDVPKDTAAVPVPADKRAPRVEAVSDKFNELVAYGEANSAENVARLPETVAAEGLPQASAMAVALPPPDSNKEQDIGGIDILNNVEEPVAQDPAEPLSEGEQLEQAIKNPLTLGGVSFKEHLKKIKPILAKGQHFHGATNERQSAVVAAFKHSWAGYKKYAWGHDNLKPISQYSHEWFGLGLTIVDSLDTMYIMGLDDEFKEGRDWVEQSLRFDTKRDVNLFEVTIRVLGGLLSAYHLSGDTMFLAKAAELGNRLLPAFQSPSNIPYSDVNLGDLSAHSPKWSPDSSTSEVTTIQLEFRDLSRSTNISIYEQVAHKVNEKVHDLEKNHGLVPIFINANTGTFRNYATISLGARGDSYYEYLLKQWIQTGRKDNDNLILDYMQAVDGVLTQLMRRTPREHWVYIGELINGKDFKPKMDHLTCYLPGTLILGHQNGMPDSHLILARDLLDTCYQTYMMNPTHLAAEISYFALTEKDDQDIYVKPNDAHNLLRPEFVESLYYFYSITGNRTYQDMGWKIFQAFETHAKVNAGYTSMGNVKNTQSTRLRDLMESFWMSETLKYFYLLFSDDRKEIDLEQWVFNSEGHPLPVYPLKT

Target ORF information:

RefSeq Version	NM_143410.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster alpha-Mannosidase class I b (alpha-Man-Ib), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143410.4

ORF Insert Sequence:

ATGAAGGACG GCGGTCACAT AACGCTCACA CTCCCGCCGG GATCAGCGGC GACGACGGAC 
GAGCGGGGCA GGAAGAGCCT GCGTCGGGCT TGGAATCAGC TACCGCGTTG TCAGCGCAAT 
CTCATTATTC TGGGCATCAC CGGATTCTGC GTGACAGTGT TGCTCTGCCT AAGTGGCGAT 
CAGCTGCTGG CTGCCAGCTT AAAGGATGTG GACACCAAGG CCGAGGTGGC AGCATATCAA 
ATGCCCCTGC CCAATCCGCA CCATCATCCG GGGGTGGATA AGGATGTGCC CAAAGACACT 
GCTGCGGTAC CTGTTCCGGC CGATAAGAGA GCACCCCGGG TGGAGGCGGT TAGTGATAAG 
TTTAATGAGC TAGTTGCCTA TGGAGAAGCG AACTCAGCGG AGAACGTGGC CCGTCTGCCG 
GAGACGGTGG CTGCTGAAGG ACTTCCCCAG GCCTCGGCCA TGGCCGTGGC CTTGCCTCCC 
CCCGATAGTA ACAAGGAGCA GGACATCGGT GGAATCGACA TCCTAAACAA TGTAGAGGAG 
CCCGTCGCCC AGGATCCAGC AGAGCCGCTT AGCGAGGGCG AGCAGCTTGA GCAAGCCATC 
AAGAACCCAC TCACTCTGGG CGGCGTTAGC TTCAAAGAGC ATCTCAAAAA GATCAAGCCC 
ATTCTGGCCA AGGGCCAGCA CTTCCATGGC GCCACCAACG AAAGACAGTC GGCTGTTGTA 
GCCGCCTTCA AGCACTCCTG GGCTGGCTAC AAGAAGTATG CCTGGGGCCA CGACAACCTG 
AAGCCCATCT CCCAGTACTC CCACGAGTGG TTCGGTCTGG GACTAACCAT TGTTGACTCA 
TTGGATACCA TGTACATAAT GGGACTGGAT GATGAATTCA AGGAAGGACG CGACTGGGTA 
GAGCAATCAC TGCGCTTTGA TACCAAGCGC GATGTAAACC TATTCGAGGT CACCATACGT 
GTCCTAGGCG GACTGCTGTC TGCATATCAC CTGAGCGGCG ATACAATGTT TCTGGCCAAG 
GCTGCGGAAC TTGGCAACCG TTTACTTCCC GCCTTTCAAT CGCCGTCAAA TATCCCCTAT 
TCTGATGTGA ATCTCGGAGA TCTTTCGGCG CACTCGCCGA AGTGGTCTCC TGATAGCTCC 
ACCAGTGAGG TCACCACCAT TCAGCTAGAG TTTCGTGATT TGAGTCGCTC TACAAACATC 
TCCATTTATG AACAGGTTGC TCACAAAGTA AACGAGAAGG TGCATGATCT GGAGAAGAAC 
CATGGTTTGG TGCCCATATT CATTAACGCC AACACAGGAA CATTCCGAAA CTACGCCACT 
ATCTCGCTTG GTGCTCGTGG GGACTCCTAC TATGAGTACT TGCTCAAACA GTGGATTCAG 
ACCGGCCGCA AGGATAATGA TAACCTTATT TTGGACTACA TGCAAGCAGT CGATGGTGTT 
CTAACACAAC TGATGCGGCG CACGCCACGC GAGCACTGGG TTTACATTGG CGAGCTGATC 
AATGGCAAAG ACTTTAAGCC GAAAATGGAT CACCTTACGT GTTATCTGCC GGGCACTTTG 
ATTCTGGGTC ACCAGAACGG AATGCCAGAC TCGCACCTTA TACTTGCGAG GGATTTATTG 
GACACATGCT ATCAGACCTA TATGATGAAT CCCACACATT TGGCTGCTGA GATCTCGTAT 
TTTGCGCTAA CCGAAAAGGA CGATCAGGAC ATTTATGTGA AGCCAAACGA TGCCCACAAC 
CTGTTGCGCC CGGAGTTTGT GGAGAGTCTC TATTACTTCT ACTCAATAAC TGGCAATCGG 
ACATACCAGG ACATGGGATG GAAAATCTTC CAGGCGTTTG AGACTCACGC TAAAGTAAAT 
GCAGGCTACA CATCGATGGG AAATGTAAAG AACACACAGA GCACGCGTCT GCGCGATCTG 
ATGGAAAGCT TTTGGATGAG CGAGACCCTG AAGTACTTCT ACCTGCTGTT TAGCGACGAC 
CGCAAGGAAA TCGACCTGGA GCAATGGGTC TTCAATTCGG AGGGCCATCC ACTGCCGGTC 
TATCCGCTCA AGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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alpha-Man-Ib cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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