CRYZL2P-SEC16B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRYZL2P-SEC16B
Entrez Gene ID	111240474
Full Name	CRYZL2P-SEC16B readthrough
General protein information	Preferred Names CRYZL2P-SEC16B readthrough Names protein transport protein Sec16B SEC16 homolog B leucine zipper transcription regulator 2 regucalcin gene promoter region-related protein p117
Gene Type	protein-coding
Organism	Homo sapiens(human)
Summary	This locus represents naturally occurring read-through transcription between the neighboring CRYZL2P (crystallin zeta like 2 pseudogene) and SEC16B (SEC16 homolog B, endoplasmic reticulum export factor) genes on chromosome 1. The readthrough transcript encodes a protein that shares sequence identity with the downstream gene product. [provided by RefSeq, Oct 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001356505.1	NP_001343434.1	protein transport protein Sec16B
NM_001356506.1	NP_001343435.1	protein transport protein Sec16B

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
Bian Y, Song C, Cheng K, Dong M, Wang F, Huang J, Sun D, Wang L, Ye M, Zou H
Journal of proteomics96253-62(2014 Jan)

Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.
Yonekawa S, Furuno A, Baba T, Fujiki Y, Ogasawara Y, Yamamoto A, Tagaya M, Tani K
Proceedings of the National Academy of Sciences of the United States of America108(31)12746-51(2011 Aug)

TFG-1 function in protein secretion and oncogenesis.
Witte K, Schuh AL, Hegermann J, Sarkeshik A, Mayers JR, Schwarze K, Yates JR 3rd, Eimer S, Audhya A
Nature cell biology13(5)550-8(2011 May)

Characterization of human Sec16B: indications of specialized, non-redundant functions.
Budnik A, Heesom KJ, Stephens DJ
Scientific reports177(2011)

The full-ORF clone resource of the German cDNA Consortium.
Bechtel S, Rosenfelder H, Duda A, Schmidt CP, Ernst U, Wellenreuther R, Mehrle A, Schuster C, Bahr A, Bl?cker H, Heubner D, Hoerlein A, Michel G, Wedler H, K?hrer K, Ottenw?lder B, Poustka A, Wiemann S, Schupp I
BMC genomics8399(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CRYZL2P-SEC16B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRYZL2P-SEC16B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu103210	NM_001356506.1 Latest version!	Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu103210	NM_001356505.1 Latest version!	Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRYZL2P-SEC16B ( NM_001356505.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343434.1 Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu103210
Clone ID Related Accession (Same CDS sequence)	NM_001356505.1 , NM_001356506.1
Accession Version	NM_001356505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL359075.15, AL356279.8 and AL160007.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.63401.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 89866, 730102 ##RefSeq-Attributes-END##

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGCAGGAGA CCCCCAGAGC ATCTTCTCCC CACCAGGCTG GCCTGGGCCT CTCACTGACA 
CCTTCCCCTG AGTCCCCACC TCTGCCGGAT GTTAGTGCCT TCTCCAGGGG CAGAGGTGGG 
GGTGAAGGCC GAGGATCCGC ATCCAGCGGG GGAGCAGCTG CGGGCGCTGG GGTTGGAGGC 
TTGTCTGGAC CAGAGAGTGT TTCCTTTGAG CTCTGCTCCA ACCCTGGTGT TCTTCTTCCT 
CCACCTGCCT TAAAAGGGGC TGTTCCTCTT TACAACCCAT CTCAGGTGCC TCAGCTGCCC 
ACGGCCACTA GCCTGAATCG GCCAAATCGC CTAGCTCAGC GTCGCTATCC CACCCAGCCA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343434.1
CDS	1047..4232
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFATTEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQGESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPHPTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQPGSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVSQETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKLGDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEQETPRASSPHQAGLGLSLTPSPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPPPALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC

Target ORF information:

RefSeq Version	NM_001356505.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356505.1

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGCAGGAGA CCCCCAGAGC ATCTTCTCCC CACCAGGCTG GCCTGGGCCT CTCACTGACA 
CCTTCCCCTG AGTCCCCACC TCTGCCGGAT GTTAGTGCCT TCTCCAGGGG CAGAGGTGGG 
GGTGAAGGCC GAGGATCCGC ATCCAGCGGG GGAGCAGCTG CGGGCGCTGG GGTTGGAGGC 
TTGTCTGGAC CAGAGAGTGT TTCCTTTGAG CTCTGCTCCA ACCCTGGTGT TCTTCTTCCT 
CCACCTGCCT TAAAAGGGGC TGTTCCTCTT TACAACCCAT CTCAGGTGCC TCAGCTGCCC 
ACGGCCACTA GCCTGAATCG GCCAAATCGC CTAGCTCAGC GTCGCTATCC CACCCAGCCA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRYZL2P-SEC16B ( NM_001356506.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001343435.1 Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu103210
Clone ID Related Accession (Same CDS sequence)	NM_001356505.1 , NM_001356506.1
Accession Version	NM_001356506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	protein transport protein Sec16B
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL359075.15, AL356279.8 and AL160007.10. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.29153.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## readthrough transcript :: includes exons from GeneID 89866, 730102 ##RefSeq-Attributes-END##

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGCAGGAGA CCCCCAGAGC ATCTTCTCCC CACCAGGCTG GCCTGGGCCT CTCACTGACA 
CCTTCCCCTG AGTCCCCACC TCTGCCGGAT GTTAGTGCCT TCTCCAGGGG CAGAGGTGGG 
GGTGAAGGCC GAGGATCCGC ATCCAGCGGG GGAGCAGCTG CGGGCGCTGG GGTTGGAGGC 
TTGTCTGGAC CAGAGAGTGT TTCCTTTGAG CTCTGCTCCA ACCCTGGTGT TCTTCTTCCT 
CCACCTGCCT TAAAAGGGGC TGTTCCTCTT TACAACCCAT CTCAGGTGCC TCAGCTGCCC 
ACGGCCACTA GCCTGAATCG GCCAAATCGC CTAGCTCAGC GTCGCTATCC CACCCAGCCA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001343435.1
CDS	711..3896
Translation	MELWAPQRLPQTRGKATAPSKDPDRGFRRDGHHRPVPHSWHNGERFHQWQDNRGSPQPQQEPRADHQQQPHYASRPGDWHQPVSGVDYYEGGYRNQLYSRPGYENSYQSYQSPTMREEYAYGSYYYHGHPQWLQEERVPRQRSPYIWHEDYREQKYLDEHHYENQHSPFGTNSETHFQSNSRNPCKDSPASNSGQEWPGELFPGSLLAEAQKNKPSLASESNLLQQRESGLSSSSYELSQYIRDAPERDDPPASAAWSPVQADVSSAGPKAPMKFYIPHVPVSFGPGGQLVHVGPSSPTDGQAALVELHSMEVILNDSEEQEEMRSFSGPLIREDVHKVDIMTFCQQKAAQSCKSETLGSRDSALLWQLLVLLCRQNGSMVGSDIAELLMQDCKKLEKYKRQPPVANLINLTDEDWPVLSSGTPNLLTGEIPPSVETPAQIVEKFTRLLYYGRKKEALEWAMKNHLWGHALFLSSKMDPQTYSWVMSGFTSTLALNDPLQTLFQLMSGRIPQAATCCGEKQWGDWRPHLAVILSNQAGDPELYQRAIVAIGDTLAGKGLVEAAHFCYLMAHVPFGHYTVKTDHLVLLGSSHSQEFLKFATTEAIQRTEIFEYCQMLGRPKSFIPSFQVYKLLYASRLADYGLVSQALHYCEAIGAAVLSQGESSHPVLLVELIKLAEKLKLSDPLVLERRSGDRDLEPDWLAQLRRQLEQKVAGDIGDPHPTRSDISGAGGTTTENTFYQDFSGCQGYSEAPGYRSALWLTPEQTCLLQPSPQQPFPLQPGSYPAGGGAGQTGTPRPFYSVPETHLPGTGSSVAVTEATGGTVWEEMLQTHLGPGENTVSQETSQPPDGQEVISKPQTPLAARPRSISESSASSAKEDEKESSDEADKNSPRNTAQRGKLGDGKEHTKSSGFGWFSWFRSKPTKNASPAGDEDSSDSPDSEQETPRASSPHQAGLGLSLTPSPESPPLPDVSAFSRGRGGGEGRGSASSGGAAAGAGVGGLSGPESVSFELCSNPGVLLPPPALKGAVPLYNPSQVPQLPTATSLNRPNRLAQRRYPTQPC

Target ORF information:

RefSeq Version	NM_001356506.1
Organism	Homo sapiens(human)
Definition	Homo sapiens CRYZL2P-SEC16B readthrough (CRYZL2P-SEC16B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356506.1

ORF Insert Sequence:

ATGGAACTTT GGGCTCCCCA GAGGCTGCCC CAGACACGAG GGAAGGCCAC AGCACCCTCA 
AAGGATCCAG ACCGAGGGTT TCGGAGAGAT GGACATCATC GGCCTGTCCC TCACTCTTGG 
CACAATGGAG AGAGGTTTCA CCAATGGCAA GACAACCGTG GGAGCCCCCA GCCACAGCAG 
GAGCCCAGGG CAGACCATCA GCAGCAGCCC CATTATGCAT CCAGGCCAGG GGACTGGCAT 
CAGCCTGTGT CTGGAGTTGA CTATTACGAA GGTGGTTATC GCAATCAGTT GTATTCAAGG 
CCAGGTTATG AGAATTCATA TCAGAGCTAT CAGTCTCCCA CAATGAGGGA GGAATATGCT 
TATGGAAGTT ATTACTATCA TGGACACCCA CAGTGGCTGC AGGAAGAAAG AGTGCCAAGG 
CAACGGAGTC CTTATATCTG GCACGAAGAT TACCGAGAGC AAAAGTACCT TGATGAACAT 
CATTATGAAA ACCAGCACAG TCCATTTGGA ACAAATAGTG AGACCCACTT CCAATCTAAC 
AGTAGGAACC CTTGTAAAGA CAGCCCTGCT TCCAACTCTG GACAGGAGTG GCCGGGGGAG 
CTGTTTCCAG GGAGCCTGCT TGCTGAGGCC CAGAAAAATA AGCCATCATT GGCTAGTGAG 
TCCAACCTTC TCCAGCAGCG TGAGTCTGGT CTCAGCTCCA GCAGCTATGA GCTCAGTCAG 
TACATCAGAG ATGCCCCGGA GCGGGATGAT CCCCCAGCTT CAGCAGCTTG GAGTCCAGTT 
CAGGCTGATG TCTCCTCAGC TGGTCCCAAA GCACCCATGA AGTTCTACAT CCCTCATGTT 
CCTGTGAGTT TCGGGCCAGG AGGTCAGCTG GTGCATGTAG GTCCCAGCTC TCCCACTGAC 
GGGCAAGCAG CCCTTGTTGA ACTGCACAGC ATGGAGGTTA TTCTTAATGA TTCCGAAGAG 
CAAGAGGAGA TGAGAAGTTT CTCAGGACCC TTGATTAGGG AAGATGTACA TAAGGTGGAT 
ATTATGACGT TTTGCCAGCA GAAAGCAGCT CAGAGCTGCA AATCTGAGAC ACTGGGGAGC 
AGAGACTCAG CTCTACTGTG GCAGCTCTTG GTTCTCCTTT GTCGCCAGAA TGGGTCCATG 
GTGGGGTCTG ACATCGCTGA GCTGCTAATG CAAGACTGCA AGAAGCTGGA GAAGTACAAG 
CGGCAGCCCC CTGTGGCCAA CCTCATCAAC CTGACCGATG AGGACTGGCC AGTGCTGAGC 
TCTGGGACCC CGAACCTGCT CACGGGAGAG ATCCCCCCCA GTGTGGAGAC ACCTGCGCAG 
ATCGTGGAGA AATTCACTAG GCTGCTCTAC TATGGAAGGA AGAAGGAAGC CTTGGAGTGG 
GCCATGAAGA ACCACTTGTG GGGCCATGCT TTGTTCCTGT CCAGCAAGAT GGACCCACAG 
ACCTACAGCT GGGTCATGAG TGGCTTCACC AGCACGCTGG CGCTCAATGA CCCACTGCAG 
ACCCTCTTCC AGCTCATGTC GGGGAGGATT CCACAGGCAG CCACGTGTTG TGGAGAAAAG 
CAGTGGGGAG ACTGGAGGCC TCACTTGGCT GTGATTCTGT CGAATCAGGC TGGGGACCCA 
GAGCTGTATC AGCGTGCGAT TGTTGCCATT GGGGACACCC TGGCTGGGAA GGGGCTTGTG 
GAGGCAGCTC ACTTCTGCTA TCTCATGGCT CACGTGCCCT TTGGCCACTA CACCGTGAAG 
ACAGACCATC TGGTCTTGCT GGGCAGCAGC CACAGTCAAG AGTTTTTGAA ATTTGCAACA 
ACTGAGGCAA TCCAGAGGAC GGAAATCTTC GAGTACTGTC AGATGCTGGG CCGCCCCAAA 
TCCTTCATCC CTTCTTTCCA GGTGTATAAG CTCCTTTATG CTTCCCGTCT GGCAGATTAT 
GGCCTCGTGT CCCAGGCTTT GCATTACTGC GAAGCCATTG GTGCAGCTGT CTTGAGCCAG 
GGAGAGAGCA GTCACCCTGT GCTATTAGTG GAACTCATCA AGCTAGCAGA GAAACTGAAG 
CTGTCAGATC CTCTGGTTTT AGAAAGACGC AGTGGAGACA GGGACCTGGA GCCAGATTGG 
CTAGCGCAAC TTCGGAGGCA GCTGGAGCAA AAGGTAGCAG GAGACATTGG GGATCCTCAT 
CCTACTCGCT CAGATATTTC GGGAGCCGGA GGAACAACAA CAGAAAACAC TTTCTACCAG 
GACTTTTCTG GATGTCAAGG CTACTCTGAA GCCCCTGGGT ACCGCTCAGC TCTGTGGCTG 
ACACCTGAGC AGACCTGCCT GCTCCAGCCC AGCCCACAGC AGCCCTTTCC CCTCCAGCCG 
GGCTCCTACC CAGCAGGAGG GGGTGCAGGG CAGACAGGGA CACCGAGGCC TTTTTACTCA 
GTTCCTGAGA CCCATCTACC AGGGACTGGC AGCAGCGTGG CAGTGACAGA GGCCACTGGA 
GGAACAGTCT GGGAGGAAAT GCTGCAGACA CACCTGGGCC CTGGAGAGAA CACAGTGTCT 
CAAGAAACTT CCCAGCCTCC TGATGGCCAA GAGGTCATTT CCAAACCACA GACACCATTG 
GCTGCTAGAC CACGAAGTAT TTCTGAGAGT TCCGCCAGTT CAGCCAAGGA GGATGAGAAG 
GAGTCCTCTG ATGAGGCTGA TAAAAACTCT CCCCGAAATA CTGCCCAGAG AGGCAAGCTC 
GGAGATGGGA AGGAGCATAC AAAGAGCTCA GGGTTTGGCT GGTTCAGCTG GTTTCGATCG 
AAGCCCACCA AGAACGCATC CCCCGCTGGA GACGAGGACT CCTCAGACAG CCCTGACTCT 
GAGCAGGAGA CCCCCAGAGC ATCTTCTCCC CACCAGGCTG GCCTGGGCCT CTCACTGACA 
CCTTCCCCTG AGTCCCCACC TCTGCCGGAT GTTAGTGCCT TCTCCAGGGG CAGAGGTGGG 
GGTGAAGGCC GAGGATCCGC ATCCAGCGGG GGAGCAGCTG CGGGCGCTGG GGTTGGAGGC 
TTGTCTGGAC CAGAGAGTGT TTCCTTTGAG CTCTGCTCCA ACCCTGGTGT TCTTCTTCCT 
CCACCTGCCT TAAAAGGGGC TGTTCCTCTT TACAACCCAT CTCAGGTGCC TCAGCTGCCC 
ACGGCCACTA GCCTGAATCG GCCAAATCGC CTAGCTCAGC GTCGCTATCC CACCCAGCCA 
TGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.
Journal of proteomics96253-62(2014 Jan)
Bian Y,Song C,Cheng K,Dong M,Wang F,Huang J,Sun D,Wang L,Ye M,Zou H

Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.
Proceedings of the National Academy of Sciences of the United States of America108(31)12746-51(2011 Aug)
Yonekawa S,Furuno A,Baba T,Fujiki Y,Ogasawara Y,Yamamoto A,Tagaya M,Tani K

TFG-1 function in protein secretion and oncogenesis.
Nature cell biology13(5)550-8(2011 May)
Witte K,Schuh AL,Hegermann J,Sarkeshik A,Mayers JR,Schwarze K,Yates JR 3rd,Eimer S,Audhya A

Characterization of human Sec16B: indications of specialized, non-redundant functions.
Scientific reports177(2011)
Budnik A,Heesom KJ,Stephens DJ

The full-ORF clone resource of the German cDNA Consortium.
BMC genomics8399(2007 Oct)
Bechtel S,Rosenfelder H,Duda A,Schmidt CP,Ernst U,Wellenreuther R,Mehrle A,Schuster C,Bahr A,Bl?cker H,Heubner D,Hoerlein A,Michel G,Wedler H,K?hrer K,Ottenw?lder B,Poustka A,Wiemann S,Schupp I

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CRYZL2P-SEC16B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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