RAMP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAMP2
Entrez Gene ID	10266
Full Name	receptor activity modifying protein 2
General protein information	Preferred Names receptor activity modifying protein 2 Names receptor activity-modifying protein 2 CRLR activity-modifying protein 2 calcitonin receptor-like receptor activity modifying protein 2 receptor (G protein-coupled) activity modifying protein 2 receptor (calcitonin) activity modifying protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.2
Summary	The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface. [provided by RefSeq, Jul 2008].
Disorder MIM:	605154

mRNA and Protein(s)

mRNA	Protein	Name
NM_005854.3	NP_005845.2	receptor activity-modifying protein 2 precursor
NM_005854.2	NP_005845.2	receptor activity-modifying protein 2 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RAMP2	XP_537636.1
	RAMP2	XP_001096145.2
Homo sapiens (human)	RAMP2	NP_005845.2
Gallus gallus (chicken)	RAMP2	NP_001099080.1
Pan troglodytes (chimpanzee)	RAMP2	XP_001160651.1
Bos taurus (cattle)	RAMP2	NP_001092330.1
Mus musculus (house mouse)	Ramp2	NP_062317.1
Rattus norvegicus (Norway rat)	Ramp2	NP_113834.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic Variants of RAMP2 and CLR are Associated with Stroke.
Koyama T, Kuriyama N, Ozaki E, Matsui D, Watanabe I, Takeshita W, Iwai K, Watanabe Y, Nakatochi M, Shimanoe C, Tanaka K, Oze I, Ito H, Uemura H, Katsuura-Kamano S, Ibusuki R, Shimoshikiryo I, Takashima N, Kadota A, Kawai S, Sasakabe T, Okada R, Hishida A, Naito M, Kuriki K, Endoh K, Furusyo N, Ikezaki H, Suzuki S, Hosono A, Mikami H, Nakamura Y, Kubo M, Wakai K
Journal of atherosclerosis and thrombosis24(12)1267-1281(2017 Dec)

RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling.
Cegla J, Jones BJ, Gardiner JV, Hodson DJ, Marjot T, McGlone ER, Tan TM, Bloom SR
Endocrinology158(8)2680-2693(2017 Aug)

N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity.
Lee SM, Booe JM, Gingell JJ, Sjoelund V, Hay DL, Pioszak AA
Biochemistry56(26)3380-3393(2017 07)

Receptor Activity-modifying Proteins 2 and 3 Generate Adrenomedullin Receptor Subtypes with Distinct Molecular Properties.
Watkins HA, Chakravarthy M, Abhayawardana RS, Gingell JJ, Garelja M, Pardamwar M, McElhinney JM, Lathbridge A, Constantine A, Harris PW, Yuen TY, Brimble MA, Barwell J, Poyner DR, Woolley MJ, Conner AC, Pioszak AA, Reynolds CA, Hay DL
The Journal of biological chemistry291(22)11657-75(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This work suggests that RAMP2 may modify the agonist activity and trafficking of the GCGR, with potential relevance to production of new peptide analogs with selective agonist activities.
Title: RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling.

Data suggest that a single GlcNAc residue at CTR N130 (asparagine 130) is responsible for enhanced affinity of calcitonin for CTR ECD; the same appears to apply for enhanced affinity of amylin for RAMP2-CTR ECD. [GlcNAc = N-acetylglucosamine; CTR = calcitonin receptor; ECD = extracellular domain; RAMP2 = receptor (calcitonin) activity modifying protein 2].
Title: N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity.

interaction of RAMP2 or RAMP3 with CLR induces conformational variation in the juxtamembrane region, yielding distinct binding pockets, probably via an allosteric mechanism.
Title: Receptor Activity-modifying Proteins 2 and 3 Generate Adrenomedullin Receptor Subtypes with Distinct Molecular Properties.

This study reveals the glucagon receptor as a previously unidentified target for GLP-1 receptor agonists and highlights a role for RAMP2 in regulating its pharmacology.
Title: Modulation of Glucagon Receptor Pharmacology by Receptor Activity-modifying Protein-2 (RAMP2).

Data suggest that ligand binding of a G protein-coupled receptor (GPCR) may inform drug development targeting calcitonin receptor-like receptor (CLR):receptor activity-modifying proteins RAMP1/2 complexes.
Title: Structural Basis for Receptor Activity-Modifying Protein-Dependent Selective Peptide Recognition by a G Protein-Coupled Receptor.

The following RAMP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAMP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30420	NM_005854.3 Latest version!	Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30420	NM_005854.2	Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAMP2 ( NM_005854.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005845.2 Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.

Price & Availability↑

CloneID	OHu30420
Clone ID Related Accession (Same CDS sequence)	NM_005854.3 , NM_005854.2
Accession Version	NM_005854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	receptor activity-modifying protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548945.3 and BC027975.1. On Nov 23, 2018 this sequence version replaced NM_005854.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL548945.3, AJ001015.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253796.10/ ENSP00000253796.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCGC TCCGGGTGGA GCGCGCCGGC GGCCCGCGTC TCCCTAGGAC CCGAGTCGGG 
CGGCCGGCAG CGCTCCGCCT CCTCCTCCTG CTGGGCGCTG TCCTGAATCC CCACGAGGCC 
CTGGCTCAGC CTCTTCCCAC CACAGGCACA CCAGGGTCAG AAGGGGGGAC GGTGAAGAAC 
TATGAGACAG CTGTCCAATT TTGCTGGAAT CATTATAAGG ATCAAATGGA TCCTATCGAA 
AAGGATTGGT GCGACTGGGC CATGATTAGC AGGCCTTATA GCACCCTGCG AGATTGCCTG 
GAGCACTTTG CAGAGTTGTT TGACCTGGGC TTCCCCAATC CCTTGGCAGA GAGGATCATC 
TTTGAGACTC ACCAGATCCA CTTTGCCAAC TGCTCCCTGG TGCAGCCCAC CTTCTCTGAC 
CCCCCAGAGG ATGTACTCCT GGCCATGATC ATAGCCCCCA TCTGCCTCAT CCCCTTCCTC 
ATCACTCTTG TAGTATGGAG GAGTAAAGAC AGTGAGGCCC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005845.2
CDS	36..563
Translation	MASLRVERAGGPRLPRTRVGRPAALRLLLLLGAVLNPHEALAQPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPIEKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Target ORF information:

RefSeq Version	NM_005854.3
Organism	Homo sapiens(human)
Definition	Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005854.3

ORF Insert Sequence:

ATGGCCTCGC TCCGGGTGGA GCGCGCCGGC GGCCCGCGTC TCCCTAGGAC CCGAGTCGGG 
CGGCCGGCAG CGCTCCGCCT CCTCCTCCTG CTGGGCGCTG TCCTGAATCC CCACGAGGCC 
CTGGCTCAGC CTCTTCCCAC CACAGGCACA CCAGGGTCAG AAGGGGGGAC GGTGAAGAAC 
TATGAGACAG CTGTCCAATT TTGCTGGAAT CATTATAAGG ATCAAATGGA TCCTATCGAA 
AAGGATTGGT GCGACTGGGC CATGATTAGC AGGCCTTATA GCACCCTGCG AGATTGCCTG 
GAGCACTTTG CAGAGTTGTT TGACCTGGGC TTCCCCAATC CCTTGGCAGA GAGGATCATC 
TTTGAGACTC ACCAGATCCA CTTTGCCAAC TGCTCCCTGG TGCAGCCCAC CTTCTCTGAC 
CCCCCAGAGG ATGTACTCCT GGCCATGATC ATAGCCCCCA TCTGCCTCAT CCCCTTCCTC 
ATCACTCTTG TAGTATGGAG GAGTAAAGAC AGTGAGGCCC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAMP2 ( NM_005854.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005845.2 Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.

Price & Availability↑

CloneID	OHu30420
Clone ID Related Accession (Same CDS sequence)	NM_005854.3 , NM_005854.2
Accession Version	NM_005854.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	receptor activity-modifying protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL548945.3 and BC027975.1. On Dec 1, 2006 this sequence version replaced NM_005854.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL548945.3, AL548919.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCCTCGC TCCGGGTGGA GCGCGCCGGC GGCCCGCGTC TCCCTAGGAC CCGAGTCGGG 
CGGCCGGCAG CGCTCCGCCT CCTCCTCCTG CTGGGCGCTG TCCTGAATCC CCACGAGGCC 
CTGGCTCAGC CTCTTCCCAC CACAGGCACA CCAGGGTCAG AAGGGGGGAC GGTGAAGAAC 
TATGAGACAG CTGTCCAATT TTGCTGGAAT CATTATAAGG ATCAAATGGA TCCTATCGAA 
AAGGATTGGT GCGACTGGGC CATGATTAGC AGGCCTTATA GCACCCTGCG AGATTGCCTG 
GAGCACTTTG CAGAGTTGTT TGACCTGGGC TTCCCCAATC CCTTGGCAGA GAGGATCATC 
TTTGAGACTC ACCAGATCCA CTTTGCCAAC TGCTCCCTGG TGCAGCCCAC CTTCTCTGAC 
CCCCCAGAGG ATGTACTCCT GGCCATGATC ATAGCCCCCA TCTGCCTCAT CCCCTTCCTC 
ATCACTCTTG TAGTATGGAG GAGTAAAGAC AGTGAGGCCC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005845.2
CDS	69..596
Translation	MASLRVERAGGPRLPRTRVGRPAALRLLLLLGAVLNPHEALAQPLPTTGTPGSEGGTVKNYETAVQFCWNHYKDQMDPIEKDWCDWAMISRPYSTLRDCLEHFAELFDLGFPNPLAERIIFETHQIHFANCSLVQPTFSDPPEDVLLAMIIAPICLIPFLITLVVWRSKDSEAQA

Target ORF information:

RefSeq Version	NM_005854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens receptor activity modifying protein 2 (RAMP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005854.2

ORF Insert Sequence:

ATGGCCTCGC TCCGGGTGGA GCGCGCCGGC GGCCCGCGTC TCCCTAGGAC CCGAGTCGGG 
CGGCCGGCAG CGCTCCGCCT CCTCCTCCTG CTGGGCGCTG TCCTGAATCC CCACGAGGCC 
CTGGCTCAGC CTCTTCCCAC CACAGGCACA CCAGGGTCAG AAGGGGGGAC GGTGAAGAAC 
TATGAGACAG CTGTCCAATT TTGCTGGAAT CATTATAAGG ATCAAATGGA TCCTATCGAA 
AAGGATTGGT GCGACTGGGC CATGATTAGC AGGCCTTATA GCACCCTGCG AGATTGCCTG 
GAGCACTTTG CAGAGTTGTT TGACCTGGGC TTCCCCAATC CCTTGGCAGA GAGGATCATC 
TTTGAGACTC ACCAGATCCA CTTTGCCAAC TGCTCCCTGG TGCAGCCCAC CTTCTCTGAC 
CCCCCAGAGG ATGTACTCCT GGCCATGATC ATAGCCCCCA TCTGCCTCAT CCCCTTCCTC 
ATCACTCTTG TAGTATGGAG GAGTAAAGAC AGTGAGGCCC AGGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic Variants of RAMP2 and CLR are Associated with Stroke.
Journal of atherosclerosis and thrombosis24(12)1267-1281(2017 Dec)
Koyama T,Kuriyama N,Ozaki E,Matsui D,Watanabe I,Takeshita W,Iwai K,Watanabe Y,Nakatochi M,Shimanoe C,Tanaka K,Oze I,Ito H,Uemura H,Katsuura-Kamano S,Ibusuki R,Shimoshikiryo I,Takashima N,Kadota A,Kawai S,Sasakabe T,Okada R,Hishida A,Naito M,Kuriki K,Endoh K,Furusyo N,Ikezaki H,Suzuki S,Hosono A,Mikami H,Nakamura Y,Kubo M,Wakai K

RAMP2 Influences Glucagon Receptor Pharmacology via Trafficking and Signaling.
Endocrinology158(8)2680-2693(2017 Aug)
Cegla J,Jones BJ,Gardiner JV,Hodson DJ,Marjot T,McGlone ER,Tan TM,Bloom SR

N-Glycosylation of Asparagine 130 in the Extracellular Domain of the Human Calcitonin Receptor Significantly Increases Peptide Hormone Affinity.
Biochemistry56(26)3380-3393(2017 07)
Lee SM,Booe JM,Gingell JJ,Sjoelund V,Hay DL,Pioszak AA

Receptor Activity-modifying Proteins 2 and 3 Generate Adrenomedullin Receptor Subtypes with Distinct Molecular Properties.
The Journal of biological chemistry291(22)11657-75(2016 May)
Watkins HA,Chakravarthy M,Abhayawardana RS,Gingell JJ,Garelja M,Pardamwar M,McElhinney JM,Lathbridge A,Constantine A,Harris PW,Yuen TY,Brimble MA,Barwell J,Poyner DR,Woolley MJ,Conner AC,Pioszak AA,Reynolds CA,Hay DL

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RAMP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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