CASP12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CASP12
Entrez Gene ID	100506742
Full Name	caspase 12 (gene/pseudogene)
Synonyms	CASP-12,CASP12P1
General protein information	Preferred Names caspase 12 (gene/pseudogene) Names inactive caspase-12 caspase 12 pseudogene 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q22.3
Summary	Caspases are cysteine proteases that cleave C-terminal aspartic acid residues on their substrate molecules. This gene is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines. In rodents, the homolog of this gene mediates apoptosis in response to endoplasmic reticulum stress. However, in humans this gene contains a polymorphism for the presence or absence of a premature stop codon. The majority of human individuals have the premature stop codon and produce a truncated non-functional protein. The read-through codon occurs primarily in individuals of African descent and carriers have endotoxin hypo-responsiveness and an increased susceptibility to severe sepsis. Several alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Feb 2011].
Disorder MIM:	608633
Disorder Html:	{Sepsis, susceptibility to} (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001191016.2	NP_001177945.2	inactive caspase-12

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Caspase 12 degrades I?B? protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion.
Chu WK, Hsu CC, Huang SF, Hsu CC, Chow SE
Oncotarget8(20)33515-33526(2017 May)

Calpain and Caspase-12 Expression in Lens Epithelial Cells of Diabetic Cataracts.
Ahn YJ, Kim MS, Chung SK
American journal of ophthalmology16731-7(2016 Jul)

The anti-inflammatory CASPASE-12 gene does not influence SLE phenotype in African-Americans.
Fuchs T, Kelly JA, Simon E, Sivils KL, Hermel E
Immunology letters17321-5(2016 05)

Population distribution of the functional caspase-12 allele.
Kachapati K, O'Brien TR, Bergeron J, Zhang M, Dean M
Human mutation27(9)975(2006 Sep)

Spread of an inactive form of caspase-12 in humans is due to recent positive selection.
Xue Y, Daly A, Yngvadottir B, Liu M, Coop G, Kim Y, Sabeti P, Chen Y, Stalker J, Huckle E, Burton J, Leonard S, Rogers J, Tyler-Smith C
American journal of human genetics78(4)659-70(2006 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endoplasmic reticulum stress may be associated with apoptosis of LECs, resulting in cataract formation in diabetic patients.
Title: Calpain and Caspase-12 Expression in Lens Epithelial Cells of Diabetic Cataracts.

CASP12 genotype thus does not influence the phenotype of systemic lupus erythematosus in African-Americans
Title: The anti-inflammatory CASPASE-12 gene does not influence SLE phenotype in African-Americans.

Expression of caspase-12 was linked to decreased systemic and adipose tissue inflammation in a cohort of African American obese children.
Title: Caspase-12, but Not Caspase-11, Inhibits Obesity and Insulin Resistance.

Endoplasmic reticulum stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy.
Title: ER stress related factor ATF6 and caspase-12 trigger apoptosis in neonatal hypoxic-ischemic encephalopathy.

We found that RNA interference-induced Caspase-12 silencing increased NOD1, hBD1 and hBD2 expression
Title: Caspase-12 silencing attenuates inhibitory effects of cigarette smoke extract on NOD1 signaling and hBDs expression in human oral mucosal epithelial cells.

The following CASP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu23440	NM_001191016.2 Latest version!	Homo sapiens caspase 12 (gene/pseudogene) (CASP12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$202.30 $289.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CASP12 ( NM_001191016.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177945.2 Homo sapiens caspase 12 (gene/pseudogene) (CASP12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23440
Clone ID Related Accession (Same CDS sequence)	NM_001191016.2
Accession Version	NM_001191016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-22
Organism	Homo sapiens(human)
Product	inactive caspase-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF464191.1, AY358222.1 and BG183061.1. On Apr 22, 2014 this sequence version replaced NM_001191016.1. Transcript Variant: This variant (1) represents the protein coding transcript, encoding Arg (CGA, aa 125) at the polymorphic site instead of the premature translation termination codon (TGA). The encoded protein (also known as Csp12-L) has no protease activity; however, it is thought to modulate inflammation and innate immune response to endotoxins, and is a risk factor for developing severe sepsis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.68909.1, AY358222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## polymorphic pseudogene :: PMID: 16532395 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGATG AGAAACCATC CAACGGTGTT CTGGTCCACA TGGTGAAGTT GCTGATCAAG 
ACCTTTCTAG ATGGCATTTT TGATGATTTG ATGGAAAATA ATGTGTTAAA TACAGATGAG 
ATACACCTTA TAGGAAAATG TCTAAAGTTT GTGGTGAGCA ATGCTGAAAA CCTGGTTGAT 
GATATCACTG AGACAGCTCA AATTGCAGGC AAAATATTTA GGGAACACCT GTGGAATTCC 
AAAAAACAGC TGAGTTCAGA TATATCCAGT GATGGAGAAA GAGAGGCGAA CATGCCTGGC 
CTCAACATCC GCAACAAAGA ATTCAACTAT CTTCATAATC GAAATGGTTC TGAACTTGAC 
CTTTTGGGGA TGCGAGATCT ACTTGAAAAC CTTGGATACT CAGTGGTTAT AAAAGAGAAT 
CTCACAGCTC AGGAAATGGA AACAGCACTA AGGCAGTTTG CTGCTCACCC AGAGCACCAG 
TCCTCAGACA GCACATTCCT GGTGTTTATG TCACATAGCA TCCTGAATGG AATCTGTGGG 
ACCAAGCACT GGGATCAAGA GCCAGATGTT CTTCACGATG ACACCATCTT TGAAATTTTC 
AACAACCGTA ACTGCCAGAG TCTGAAAGAC AAACCCAAGG TCATCATCAT GCAAGCCTGC 
CGAGGCAATG GTGCTGGGAT TGTTTGGTTC ACCACTGACA GTGGAAAAGC CAGTGCAGAT 
ACTCATGGTC GGCTCTTGCA AGGTAACATC TGTAATGATG CTGTTACAAA GGCTCATGTG 
GAAAAGGACT TCATTGCTTT CAAATCTTCC ACACCACATA ATGTTTCTTG GAGACATGAA 
ACAAATGGCT CTGTCTTCAT TTCCCAAATT ATCTACTACT TCAGAGAGTA TTCTTGGAGT 
CATCATCTAG AGGAAATTTT TCAAAAGGTT CAACATTCAT TTGAGACCCC AAATATACTG 
ACCCAGCTGC CCACCATTGA AAGACTATCC ATGACACGAT ATTTCTATCT CTTTCCTGGG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177945.2
CDS	257..1282
Translation	MADEKPSNGVLVHMVKLLIKTFLDGIFDDLMENNVLNTDEIHLIGKCLKFVVSNAENLVDDITETAQIAGKIFREHLWNSKKQLSSDISSDGEREANMPGLNIRNKEFNYLHNRNGSELDLLGMRDLLENLGYSVVIKENLTAQEMETALRQFAAHPEHQSSDSTFLVFMSHSILNGICGTKHWDQEPDVLHDDTIFEIFNNRNCQSLKDKPKVIIMQACRGNGAGIVWFTTDSGKASADTHGRLLQGNICNDAVTKAHVEKDFIAFKSSTPHNVSWRHETNGSVFISQIIYYFREYSWSHHLEEIFQKVQHSFETPNILTQLPTIERLSMTRYFYLFPGN

Target ORF information:

RefSeq Version	NM_001191016.2
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 12 (gene/pseudogene) (CASP12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191016.2

ORF Insert Sequence:

ATGGCTGATG AGAAACCATC CAACGGTGTT CTGGTCCACA TGGTGAAGTT GCTGATCAAG 
ACCTTTCTAG ATGGCATTTT TGATGATTTG ATGGAAAATA ATGTGTTAAA TACAGATGAG 
ATACACCTTA TAGGAAAATG TCTAAAGTTT GTGGTGAGCA ATGCTGAAAA CCTGGTTGAT 
GATATCACTG AGACAGCTCA AATTGCAGGC AAAATATTTA GGGAACACCT GTGGAATTCC 
AAAAAACAGC TGAGTTCAGA TATATCCAGT GATGGAGAAA GAGAGGCGAA CATGCCTGGC 
CTCAACATCC GCAACAAAGA ATTCAACTAT CTTCATAATC GAAATGGTTC TGAACTTGAC 
CTTTTGGGGA TGCGAGATCT ACTTGAAAAC CTTGGATACT CAGTGGTTAT AAAAGAGAAT 
CTCACAGCTC AGGAAATGGA AACAGCACTA AGGCAGTTTG CTGCTCACCC AGAGCACCAG 
TCCTCAGACA GCACATTCCT GGTGTTTATG TCACATAGCA TCCTGAATGG AATCTGTGGG 
ACCAAGCACT GGGATCAAGA GCCAGATGTT CTTCACGATG ACACCATCTT TGAAATTTTC 
AACAACCGTA ACTGCCAGAG TCTGAAAGAC AAACCCAAGG TCATCATCAT GCAAGCCTGC 
CGAGGCAATG GTGCTGGGAT TGTTTGGTTC ACCACTGACA GTGGAAAAGC CAGTGCAGAT 
ACTCATGGTC GGCTCTTGCA AGGTAACATC TGTAATGATG CTGTTACAAA GGCTCATGTG 
GAAAAGGACT TCATTGCTTT CAAATCTTCC ACACCACATA ATGTTTCTTG GAGACATGAA 
ACAAATGGCT CTGTCTTCAT TTCCCAAATT ATCTACTACT TCAGAGAGTA TTCTTGGAGT 
CATCATCTAG AGGAAATTTT TCAAAAGGTT CAACATTCAT TTGAGACCCC AAATATACTG 
ACCCAGCTGC CCACCATTGA AAGACTATCC ATGACACGAT ATTTCTATCT CTTTCCTGGG 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Caspase 12 degrades I?B? protein and enhances MMP-9 expression in human nasopharyngeal carcinoma cell invasion.
Oncotarget8(20)33515-33526(2017 May)
Chu WK,Hsu CC,Huang SF,Hsu CC,Chow SE

Calpain and Caspase-12 Expression in Lens Epithelial Cells of Diabetic Cataracts.
American journal of ophthalmology16731-7(2016 Jul)
Ahn YJ,Kim MS,Chung SK

The anti-inflammatory CASPASE-12 gene does not influence SLE phenotype in African-Americans.
Immunology letters17321-5(2016 05)
Fuchs T,Kelly JA,Simon E,Sivils KL,Hermel E

Population distribution of the functional caspase-12 allele.
Human mutation27(9)975(2006 Sep)
Kachapati K,O'Brien TR,Bergeron J,Zhang M,Dean M

Spread of an inactive form of caspase-12 in humans is due to recent positive selection.
American journal of human genetics78(4)659-70(2006 Apr)
Xue Y,Daly A,Yngvadottir B,Liu M,Coop G,Kim Y,Sabeti P,Chen Y,Stalker J,Huckle E,Burton J,Leonard S,Rogers J,Tyler-Smith C

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CASP12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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