rad54b cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder)

Gene
Clones
gRNAs

Summary

Gene Symbol	rad54b
Entrez Gene ID	102303483
Full Name	RAD54 homolog B (S. cerevisiae)
Gene Type	protein-coding
Organism	Haplochromis burtoni(Burton's mouthbrooder)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014338492.1	XP_014193978.1	DNA repair and recombination protein RAD54B isoform X2
XM_005937583.1	XP_005937645.1	DNA repair and recombination protein RAD54B isoform X1

Pathways from BioSystems
Gene Ontology

The following rad54b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rad54b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHa23063	XM_005937583.1 Latest version!	Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OHa23064	XM_014338492.1 Latest version!	Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rad54b ( XM_005937583.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_005937645.1 Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHa23063
Clone ID Related Accession (Same CDS sequence)	XM_005937583.1
Accession Version	XM_005937583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	DNA repair and recombination protein RAD54B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179629.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCT CTGGAGCTCC CAGCAAGCTC TTGGGTAATG CAGTGAAAAA GTCTCGATTT 
GTGCCACCTG GTGCATGTAC TTCACTTTCT GTGGCTCAAT CCAAGCCTTT GACCCCCAAA 
CTTGGTTCAG GCAATGCACT GGATAAAGTC CAGAGAAGCC TTGCAACACC AGGTGCAAGT 
AAGACAGAGT TTAACGTCCA GCCTGAAGCT GCCCCAGCCT TGTCAAAGGT TCTGGCTCGT 
GTTCTCAGTG CAACTGAGAG TAAGGAAAAT GAGGCTGAGA CAAAGCATCA GAACCCTGGA 
TTAGGAGCCT CTGCTGAAGA CTATAAACCA GCAGGAGTGA ATGATGGCCC TCCTGGGTCT 
CCCCAAACCC CTCAGCCTCC TGCAGGCTCA GACTCTGGCT GCAGTCAGGA CGGGGCGCGT 
TACTTCAGTG TGGTGTGGTG TAAGGCCAGC AAGAAGAAAC ATAAGCGCTG GGAGGGAGAC 
GCAGTGCTGG TGACAAGAGG ACGCACAGTC ACTCTTAAAG ATATGGAGGG GAAGGATATC 
GGAAAGGGTA GTGGCTACAA GGTGTCGGAG CTGGCCTCGC TTTCCGAGGG AGAGACTCTC 
ATGATTGGTG GGAAAGAGGT GGAGGTGATG GGAGTCATTT CTGCTGAGGA CTTTGCCAAG 
GGGCGCTGTT TTCAGGAGGT CCAAACAGAG CGAGTGGAGT TAAACACAAA GTCTGCTCCA 
CCACCCTCCT GCCGCTTGGC ATTCAAACCT TTTTGCCCTC CAACAATGGT GGGCGGAGCA 
GGCCACAGAG AAACAAAACC AGAGGAGGAA CAAACTCGCA GACCTCGCCA TGACCCCCTC 
GCTCCAGGTG CACTGGTGAT GCCTCGCCCT TCCGCCAACC ACCAGTGGTT AAATAACAAG 
TCAGGGCTTC CTATGGTCGA TGTTGTGCTT GACCCACACC TGACCACACA CCTGCGACCG 
CACCAGAGGG ATGGCCTGCT CTTCCTCTAC GAGTGTGTCA TGGGCATGAG AGCTGCAGGT 
TGCTACGGTG CGATCCTGGC CGACGAAATG GGTCTGGGAA AGACTCTCCA GAGCGTGGCG 
CTGTCTTGGA CGTTGCTGAA GCAGGGACCT TATGGTGGGA GGCCAGTTGC TAAGCGTGTG 
CTTGTGGTTA CACCTGGCAG CCTGGTACAG AACTGGGCCG CAGAATTCAA CAAGTGGCTG 
GGCCGTGAGA GGATCAGTGT TTTCACTGTG GATCAGGACC ACAGGATAGA GCAGTTCGTT 
TTGTCCCCAC TGCACACCGT TCTTGTGATA AGCTATGAAA TGCTGCTGCG CTGCCTGGAG 
CAGGTCCAGA AAGTTGATTT TGGTCTTATA ATCTGTGATG AGGGTCACAG ATTAAAGAAT 
AGCAACATCA AAACATCATC AGCCCTCAGC AGCCTCAAGT GTAATCGCAG AGTCATACTT 
ACAGGCACTC CAGTTCAAAA CGACCTGCAG GAGTTCTACG CCATCATTGA GTTTGTGAAC 
CCTGGGATTC TGGGCTCATC GACTGCTTAT AGAAAAGTAT ACGAGGAACC AATCCTGCGC 
TCCAGACAGC CCGCATGCAC AGAGGAGGAG AGAGTTTTGG GAGAGGAGCG AGCGGCCGAG 
CTCTCTCGTC TGACTGGCAT GTTCATCTTG AGGCGGACGC AGGAAATCAT CAACCGCTAC 
CTGCCTCCTC GCCTCGACTG GACGTTGTTT TGTGAGCCGT CCCCTCTGCA ACGAGAGCTG 
TACAAGCACC TCCTCTGCCA CAGAGTCTTC AGGGCCTGCC TTCAGGGCTC CACTCAAACC 
CACACACATC TGGCTTGCAT CACTGCACTG AAAAAGCTCT GTAATCACCC CGGACTGCTC 
CACGTCACTG TTAAGGAGAG GACAGATGCT GGCTCTGTGG AGAGTTCATT ATATGAGGGG 
CTGGCAGATC TCTTCCCAGA ATCCTATTCT TCAGGAGGAT TCACCATGGC TGACTCTGGA 
AAACTGCTGG TTCTTACGGA CCTGCTTGCC GCCATTAGAC AGCTGAGCTC TTCAGACAGG 
GTGGTCGTAG TGTCAAACTA CACTCGGACG CTAGACTTGC TCCAGGACCT GTGTGTACAC 
ATGGGCTACA CCTTCTGTCG GCTGGACGGA CATACCCCCA CGAACCAGAG GCAGCGACTG 
GTCGACAGTT TCAACAGTCC CTATTCTCAG AACTTCCTGT TTCTGCTGAG CTCTAAAGCA 
GGTGGGGTTG GGCTTAATCT CATTGGCGCT TCCCACCTGG TGCTATACGA CATCGACTGG 
AACCCTGCCA ACGACATTCA GGCCATGGCT CGGGTGTGGA GAGATGGACA GAAGAAGACT 
GTGCACATCT ACCGTCTTCT CACTGCAGGC ACTATTGAGG AGCGTATATT TCAGCGACAG 
GTGTCCAAAC AGGGACTCTC TGGAACCGTG GTTGACATGG GGAAAGGGGC AGAGCACACG 
AGTTTCTCCA CCAGTGACCT GCGAGATCTT TTCAGCCTGA CGGAGACGCC CTGCCTGACT 
CATGACCTGC TGAACTGTGG CTGCGGCATG GATGGCACAC TCAGCGCTAT CACAGAGGAG 
GAGGAGCTGG TTTCCGAACG GCCCTGCCAG CTCGGTCGTC AAGGTGACCG TAAGGGGGCG 
ACACAGAAGC ATCTCAGCAT GTCCGAGCTC ATGCAGTGGA GACATTTCTC CGGTGACACA 
CACTCCTTCT CTGACCCCTA CCTCGATCAC GCGCGAAACA ACATCACTTT CGCCTTCCAG 
ACCACCATCT CTCACGCAAC CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005937645.1
CDS	121..2907
Translation	MRRSGAPSKLLGNAVKKSRFVPPGACTSLSVAQSKPLTPKLGSGNALDKVQRSLATPGASKTEFNVQPEAAPALSKVLARVLSATESKENEAETKHQNPGLGASAEDYKPAGVNDGPPGSPQTPQPPAGSDSGCSQDGARYFSVVWCKASKKKHKRWEGDAVLVTRGRTVTLKDMEGKDIGKGSGYKVSELASLSEGETLMIGGKEVEVMGVISAEDFAKGRCFQEVQTERVELNTKSAPPPSCRLAFKPFCPPTMVGGAGHRETKPEEEQTRRPRHDPLAPGALVMPRPSANHQWLNNKSGLPMVDVVLDPHLTTHLRPHQRDGLLFLYECVMGMRAAGCYGAILADEMGLGKTLQSVALSWTLLKQGPYGGRPVAKRVLVVTPGSLVQNWAAEFNKWLGRERISVFTVDQDHRIEQFVLSPLHTVLVISYEMLLRCLEQVQKVDFGLIICDEGHRLKNSNIKTSSALSSLKCNRRVILTGTPVQNDLQEFYAIIEFVNPGILGSSTAYRKVYEEPILRSRQPACTEEERVLGEERAAELSRLTGMFILRRTQEIINRYLPPRLDWTLFCEPSPLQRELYKHLLCHRVFRACLQGSTQTHTHLACITALKKLCNHPGLLHVTVKERTDAGSVESSLYEGLADLFPESYSSGGFTMADSGKLLVLTDLLAAIRQLSSSDRVVVVSNYTRTLDLLQDLCVHMGYTFCRLDGHTPTNQRQRLVDSFNSPYSQNFLFLLSSKAGGVGLNLIGASHLVLYDIDWNPANDIQAMARVWRDGQKKTVHIYRLLTAGTIEERIFQRQVSKQGLSGTVVDMGKGAEHTSFSTSDLRDLFSLTETPCLTHDLLNCGCGMDGTLSAITEEEELVSERPCQLGRQGDRKGATQKHLSMSELMQWRHFSGDTHSFSDPYLDHARNNITFAFQTTISHATQ

Target ORF information:

RefSeq Version	XM_005937583.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005937583.1

ORF Insert Sequence:

ATGCGGCGCT CTGGAGCTCC CAGCAAGCTC TTGGGTAATG CAGTGAAAAA GTCTCGATTT 
GTGCCACCTG GTGCATGTAC TTCACTTTCT GTGGCTCAAT CCAAGCCTTT GACCCCCAAA 
CTTGGTTCAG GCAATGCACT GGATAAAGTC CAGAGAAGCC TTGCAACACC AGGTGCAAGT 
AAGACAGAGT TTAACGTCCA GCCTGAAGCT GCCCCAGCCT TGTCAAAGGT TCTGGCTCGT 
GTTCTCAGTG CAACTGAGAG TAAGGAAAAT GAGGCTGAGA CAAAGCATCA GAACCCTGGA 
TTAGGAGCCT CTGCTGAAGA CTATAAACCA GCAGGAGTGA ATGATGGCCC TCCTGGGTCT 
CCCCAAACCC CTCAGCCTCC TGCAGGCTCA GACTCTGGCT GCAGTCAGGA CGGGGCGCGT 
TACTTCAGTG TGGTGTGGTG TAAGGCCAGC AAGAAGAAAC ATAAGCGCTG GGAGGGAGAC 
GCAGTGCTGG TGACAAGAGG ACGCACAGTC ACTCTTAAAG ATATGGAGGG GAAGGATATC 
GGAAAGGGTA GTGGCTACAA GGTGTCGGAG CTGGCCTCGC TTTCCGAGGG AGAGACTCTC 
ATGATTGGTG GGAAAGAGGT GGAGGTGATG GGAGTCATTT CTGCTGAGGA CTTTGCCAAG 
GGGCGCTGTT TTCAGGAGGT CCAAACAGAG CGAGTGGAGT TAAACACAAA GTCTGCTCCA 
CCACCCTCCT GCCGCTTGGC ATTCAAACCT TTTTGCCCTC CAACAATGGT GGGCGGAGCA 
GGCCACAGAG AAACAAAACC AGAGGAGGAA CAAACTCGCA GACCTCGCCA TGACCCCCTC 
GCTCCAGGTG CACTGGTGAT GCCTCGCCCT TCCGCCAACC ACCAGTGGTT AAATAACAAG 
TCAGGGCTTC CTATGGTCGA TGTTGTGCTT GACCCACACC TGACCACACA CCTGCGACCG 
CACCAGAGGG ATGGCCTGCT CTTCCTCTAC GAGTGTGTCA TGGGCATGAG AGCTGCAGGT 
TGCTACGGTG CGATCCTGGC CGACGAAATG GGTCTGGGAA AGACTCTCCA GAGCGTGGCG 
CTGTCTTGGA CGTTGCTGAA GCAGGGACCT TATGGTGGGA GGCCAGTTGC TAAGCGTGTG 
CTTGTGGTTA CACCTGGCAG CCTGGTACAG AACTGGGCCG CAGAATTCAA CAAGTGGCTG 
GGCCGTGAGA GGATCAGTGT TTTCACTGTG GATCAGGACC ACAGGATAGA GCAGTTCGTT 
TTGTCCCCAC TGCACACCGT TCTTGTGATA AGCTATGAAA TGCTGCTGCG CTGCCTGGAG 
CAGGTCCAGA AAGTTGATTT TGGTCTTATA ATCTGTGATG AGGGTCACAG ATTAAAGAAT 
AGCAACATCA AAACATCATC AGCCCTCAGC AGCCTCAAGT GTAATCGCAG AGTCATACTT 
ACAGGCACTC CAGTTCAAAA CGACCTGCAG GAGTTCTACG CCATCATTGA GTTTGTGAAC 
CCTGGGATTC TGGGCTCATC GACTGCTTAT AGAAAAGTAT ACGAGGAACC AATCCTGCGC 
TCCAGACAGC CCGCATGCAC AGAGGAGGAG AGAGTTTTGG GAGAGGAGCG AGCGGCCGAG 
CTCTCTCGTC TGACTGGCAT GTTCATCTTG AGGCGGACGC AGGAAATCAT CAACCGCTAC 
CTGCCTCCTC GCCTCGACTG GACGTTGTTT TGTGAGCCGT CCCCTCTGCA ACGAGAGCTG 
TACAAGCACC TCCTCTGCCA CAGAGTCTTC AGGGCCTGCC TTCAGGGCTC CACTCAAACC 
CACACACATC TGGCTTGCAT CACTGCACTG AAAAAGCTCT GTAATCACCC CGGACTGCTC 
CACGTCACTG TTAAGGAGAG GACAGATGCT GGCTCTGTGG AGAGTTCATT ATATGAGGGG 
CTGGCAGATC TCTTCCCAGA ATCCTATTCT TCAGGAGGAT TCACCATGGC TGACTCTGGA 
AAACTGCTGG TTCTTACGGA CCTGCTTGCC GCCATTAGAC AGCTGAGCTC TTCAGACAGG 
GTGGTCGTAG TGTCAAACTA CACTCGGACG CTAGACTTGC TCCAGGACCT GTGTGTACAC 
ATGGGCTACA CCTTCTGTCG GCTGGACGGA CATACCCCCA CGAACCAGAG GCAGCGACTG 
GTCGACAGTT TCAACAGTCC CTATTCTCAG AACTTCCTGT TTCTGCTGAG CTCTAAAGCA 
GGTGGGGTTG GGCTTAATCT CATTGGCGCT TCCCACCTGG TGCTATACGA CATCGACTGG 
AACCCTGCCA ACGACATTCA GGCCATGGCT CGGGTGTGGA GAGATGGACA GAAGAAGACT 
GTGCACATCT ACCGTCTTCT CACTGCAGGC ACTATTGAGG AGCGTATATT TCAGCGACAG 
GTGTCCAAAC AGGGACTCTC TGGAACCGTG GTTGACATGG GGAAAGGGGC AGAGCACACG 
AGTTTCTCCA CCAGTGACCT GCGAGATCTT TTCAGCCTGA CGGAGACGCC CTGCCTGACT 
CATGACCTGC TGAACTGTGG CTGCGGCATG GATGGCACAC TCAGCGCTAT CACAGAGGAG 
GAGGAGCTGG TTTCCGAACG GCCCTGCCAG CTCGGTCGTC AAGGTGACCG TAAGGGGGCG 
ACACAGAAGC ATCTCAGCAT GTCCGAGCTC ATGCAGTGGA GACATTTCTC CGGTGACACA 
CACTCCTTCT CTGACCCCTA CCTCGATCAC GCGCGAAACA ACATCACTTT CGCCTTCCAG 
ACCACCATCT CTCACGCAAC CCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rad54b ( XM_014338492.1 ) cDNA ORF clone, Haplochromis burtoni(Burton's mouthbrooder) -> XP_014193978.1 Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHa23064
Clone ID Related Accession (Same CDS sequence)	XM_014338492.1
Accession Version	XM_014338492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2265bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-28
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Product	DNA repair and recombination protein RAD54B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005179629.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Haplochromis burtoni Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGGA AGGATATCGG AAAGGGTAGT GGCTACAAGG TGTCGGAGCT GGCCTCGCTT 
TCCGAGGGAG AGACTCTCAT GATTGGTGGG AAAGAGGTGG AGGTGATGGG AGTCATTTCT 
GCTGAGGACT TTGCCAAGGG GCGCTGTTTT CAGGAGGTCC AAACAGAGCG AGTGGAGTTA 
AACACAAAGT CTGCTCCACC ACCCTCCTGC CGCTTGGCAT TCAAACCTTT TTGCCCTCCA 
ACAATGGTGG GCGGAGCAGG CCACAGAGAA ACAAAACCAG AGGAGGAACA AACTCGCAGA 
CCTCGCCATG ACCCCCTCGC TCCAGGTGCA CTGGTGATGC CTCGCCCTTC CGCCAACCAC 
CAGTGGTTAA ATAACAAGTC AGGGCTTCCT ATGGTCGATG TTGTGCTTGA CCCACACCTG 
ACCACACACC TGCGACCGCA CCAGAGGGAT GGCCTGCTCT TCCTCTACGA GTGTGTCATG 
GGCATGAGAG CTGCAGGTTG CTACGGTGCG ATCCTGGCCG ACGAAATGGG TCTGGGAAAG 
ACTCTCCAGA GCGTGGCGCT GTCTTGGACG TTGCTGAAGC AGGGACCTTA TGGTGGGAGG 
CCAGTTGCTA AGCGTGTGCT TGTGGTTACA CCTGGCAGCC TGGTACAGAA CTGGGCCGCA 
GAATTCAACA AGTGGCTGGG CCGTGAGAGG ATCAGTGTTT TCACTGTGGA TCAGGACCAC 
AGGATAGAGC AGTTCGTTTT GTCCCCACTG CACACCGTTC TTGTGATAAG CTATGAAATG 
CTGCTGCGCT GCCTGGAGCA GGTCCAGAAA GTTGATTTTG GTCTTATAAT CTGTGATGAG 
GGTCACAGAT TAAAGAATAG CAACATCAAA ACATCATCAG CCCTCAGCAG CCTCAAGTGT 
AATCGCAGAG TCATACTTAC AGGCACTCCA GTTCAAAACG ACCTGCAGGA GTTCTACGCC 
ATCATTGAGT TTGTGAACCC TGGGATTCTG GGCTCATCGA CTGCTTATAG AAAAGTATAC 
GAGGAACCAA TCCTGCGCTC CAGACAGCCC GCATGCACAG AGGAGGAGAG AGTTTTGGGA 
GAGGAGCGAG CGGCCGAGCT CTCTCGTCTG ACTGGCATGT TCATCTTGAG GCGGACGCAG 
GAAATCATCA ACCGCTACCT GCCTCCTCGC CTCGACTGGA CGTTGTTTTG TGAGCCGTCC 
CCTCTGCAAC GAGAGCTGTA CAAGCACCTC CTCTGCCACA GAGTCTTCAG GGCCTGCCTT 
CAGGGCTCCA CTCAAACCCA CACACATCTG GCTTGCATCA CTGCACTGAA AAAGCTCTGT 
AATCACCCCG GACTGCTCCA CGTCACTGTT AAGGAGAGGA CAGATGCTGG CTCTGTGGAG 
AGTTCATTAT ATGAGGGGCT GGCAGATCTC TTCCCAGAAT CCTATTCTTC AGGAGGATTC 
ACCATGGCTG ACTCTGGAAA ACTGCTGGTT CTTACGGACC TGCTTGCCGC CATTAGACAG 
CTGAGCTCTT CAGACAGGGT GGTCGTAGTG TCAAACTACA CTCGGACGCT AGACTTGCTC 
CAGGACCTGT GTGTACACAT GGGCTACACC TTCTGTCGGC TGGACGGACA TACCCCCACG 
AACCAGAGGC AGCGACTGGT CGACAGTTTC AACAGTCCCT ATTCTCAGAA CTTCCTGTTT 
CTGCTGAGCT CTAAAGCAGG TGGGGTTGGG CTTAATCTCA TTGGCGCTTC CCACCTGGTG 
CTATACGACA TCGACTGGAA CCCTGCCAAC GACATTCAGG CCATGGCTCG GGTGTGGAGA 
GATGGACAGA AGAAGACTGT GCACATCTAC CGTCTTCTCA CTGCAGGCAC TATTGAGGAG 
CGTATATTTC AGCGACAGGT GTCCAAACAG GGACTCTCTG GAACCGTGGT TGACATGGGG 
AAAGGGGCAG AGCACACGAG TTTCTCCACC AGTGACCTGC GAGATCTTTT CAGCCTGACG 
GAGACGCCCT GCCTGACTCA TGACCTGCTG AACTGTGGCT GCGGCATGGA TGGCACACTC 
AGCGCTATCA CAGAGGAGGA GGAGCTGGTT TCCGAACGGC CCTGCCAGCT CGGTCGTCAA 
GGTGACCGTA AGGGGGCGAC ACAGAAGCAT CTCAGCATGT CCGAGCTCAT GCAGTGGAGA 
CATTTCTCCG GTGACACACA CTCCTTCTCT GACCCCTACC TCGATCACGC GCGAAACAAC 
ATCACTTTCG CCTTCCAGAC CACCATCTCT CACGCAACCC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014193978.1
CDS	243..2507
Translation	MEGKDIGKGSGYKVSELASLSEGETLMIGGKEVEVMGVISAEDFAKGRCFQEVQTERVELNTKSAPPPSCRLAFKPFCPPTMVGGAGHRETKPEEEQTRRPRHDPLAPGALVMPRPSANHQWLNNKSGLPMVDVVLDPHLTTHLRPHQRDGLLFLYECVMGMRAAGCYGAILADEMGLGKTLQSVALSWTLLKQGPYGGRPVAKRVLVVTPGSLVQNWAAEFNKWLGRERISVFTVDQDHRIEQFVLSPLHTVLVISYEMLLRCLEQVQKVDFGLIICDEGHRLKNSNIKTSSALSSLKCNRRVILTGTPVQNDLQEFYAIIEFVNPGILGSSTAYRKVYEEPILRSRQPACTEEERVLGEERAAELSRLTGMFILRRTQEIINRYLPPRLDWTLFCEPSPLQRELYKHLLCHRVFRACLQGSTQTHTHLACITALKKLCNHPGLLHVTVKERTDAGSVESSLYEGLADLFPESYSSGGFTMADSGKLLVLTDLLAAIRQLSSSDRVVVVSNYTRTLDLLQDLCVHMGYTFCRLDGHTPTNQRQRLVDSFNSPYSQNFLFLLSSKAGGVGLNLIGASHLVLYDIDWNPANDIQAMARVWRDGQKKTVHIYRLLTAGTIEERIFQRQVSKQGLSGTVVDMGKGAEHTSFSTSDLRDLFSLTETPCLTHDLLNCGCGMDGTLSAITEEEELVSERPCQLGRQGDRKGATQKHLSMSELMQWRHFSGDTHSFSDPYLDHARNNITFAFQTTISHATQ

Target ORF information:

RefSeq Version	XM_014338492.1
Organism	Haplochromis burtoni(Burton's mouthbrooder)
Definition	Haplochromis burtoni RAD54 homolog B (S. cerevisiae) (rad54b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014338492.1

ORF Insert Sequence:

ATGGAGGGGA AGGATATCGG AAAGGGTAGT GGCTACAAGG TGTCGGAGCT GGCCTCGCTT 
TCCGAGGGAG AGACTCTCAT GATTGGTGGG AAAGAGGTGG AGGTGATGGG AGTCATTTCT 
GCTGAGGACT TTGCCAAGGG GCGCTGTTTT CAGGAGGTCC AAACAGAGCG AGTGGAGTTA 
AACACAAAGT CTGCTCCACC ACCCTCCTGC CGCTTGGCAT TCAAACCTTT TTGCCCTCCA 
ACAATGGTGG GCGGAGCAGG CCACAGAGAA ACAAAACCAG AGGAGGAACA AACTCGCAGA 
CCTCGCCATG ACCCCCTCGC TCCAGGTGCA CTGGTGATGC CTCGCCCTTC CGCCAACCAC 
CAGTGGTTAA ATAACAAGTC AGGGCTTCCT ATGGTCGATG TTGTGCTTGA CCCACACCTG 
ACCACACACC TGCGACCGCA CCAGAGGGAT GGCCTGCTCT TCCTCTACGA GTGTGTCATG 
GGCATGAGAG CTGCAGGTTG CTACGGTGCG ATCCTGGCCG ACGAAATGGG TCTGGGAAAG 
ACTCTCCAGA GCGTGGCGCT GTCTTGGACG TTGCTGAAGC AGGGACCTTA TGGTGGGAGG 
CCAGTTGCTA AGCGTGTGCT TGTGGTTACA CCTGGCAGCC TGGTACAGAA CTGGGCCGCA 
GAATTCAACA AGTGGCTGGG CCGTGAGAGG ATCAGTGTTT TCACTGTGGA TCAGGACCAC 
AGGATAGAGC AGTTCGTTTT GTCCCCACTG CACACCGTTC TTGTGATAAG CTATGAAATG 
CTGCTGCGCT GCCTGGAGCA GGTCCAGAAA GTTGATTTTG GTCTTATAAT CTGTGATGAG 
GGTCACAGAT TAAAGAATAG CAACATCAAA ACATCATCAG CCCTCAGCAG CCTCAAGTGT 
AATCGCAGAG TCATACTTAC AGGCACTCCA GTTCAAAACG ACCTGCAGGA GTTCTACGCC 
ATCATTGAGT TTGTGAACCC TGGGATTCTG GGCTCATCGA CTGCTTATAG AAAAGTATAC 
GAGGAACCAA TCCTGCGCTC CAGACAGCCC GCATGCACAG AGGAGGAGAG AGTTTTGGGA 
GAGGAGCGAG CGGCCGAGCT CTCTCGTCTG ACTGGCATGT TCATCTTGAG GCGGACGCAG 
GAAATCATCA ACCGCTACCT GCCTCCTCGC CTCGACTGGA CGTTGTTTTG TGAGCCGTCC 
CCTCTGCAAC GAGAGCTGTA CAAGCACCTC CTCTGCCACA GAGTCTTCAG GGCCTGCCTT 
CAGGGCTCCA CTCAAACCCA CACACATCTG GCTTGCATCA CTGCACTGAA AAAGCTCTGT 
AATCACCCCG GACTGCTCCA CGTCACTGTT AAGGAGAGGA CAGATGCTGG CTCTGTGGAG 
AGTTCATTAT ATGAGGGGCT GGCAGATCTC TTCCCAGAAT CCTATTCTTC AGGAGGATTC 
ACCATGGCTG ACTCTGGAAA ACTGCTGGTT CTTACGGACC TGCTTGCCGC CATTAGACAG 
CTGAGCTCTT CAGACAGGGT GGTCGTAGTG TCAAACTACA CTCGGACGCT AGACTTGCTC 
CAGGACCTGT GTGTACACAT GGGCTACACC TTCTGTCGGC TGGACGGACA TACCCCCACG 
AACCAGAGGC AGCGACTGGT CGACAGTTTC AACAGTCCCT ATTCTCAGAA CTTCCTGTTT 
CTGCTGAGCT CTAAAGCAGG TGGGGTTGGG CTTAATCTCA TTGGCGCTTC CCACCTGGTG 
CTATACGACA TCGACTGGAA CCCTGCCAAC GACATTCAGG CCATGGCTCG GGTGTGGAGA 
GATGGACAGA AGAAGACTGT GCACATCTAC CGTCTTCTCA CTGCAGGCAC TATTGAGGAG 
CGTATATTTC AGCGACAGGT GTCCAAACAG GGACTCTCTG GAACCGTGGT TGACATGGGG 
AAAGGGGCAG AGCACACGAG TTTCTCCACC AGTGACCTGC GAGATCTTTT CAGCCTGACG 
GAGACGCCCT GCCTGACTCA TGACCTGCTG AACTGTGGCT GCGGCATGGA TGGCACACTC 
AGCGCTATCA CAGAGGAGGA GGAGCTGGTT TCCGAACGGC CCTGCCAGCT CGGTCGTCAA 
GGTGACCGTA AGGGGGCGAC ACAGAAGCAT CTCAGCATGT CCGAGCTCAT GCAGTGGAGA 
CATTTCTCCG GTGACACACA CTCCTTCTCT GACCCCTACC TCGATCACGC GCGAAACAAC 
ATCACTTTCG CCTTCCAGAC CACCATCTCT CACGCAACCC AGTAA