LOC105772811 cDNA ORF clone, Gossypium raimondii

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105772811
Entrez Gene ID	105772811
Full Name	DNA-directed RNA polymerase II subunit RPB2
Gene Type	protein-coding
Organism	Gossypium raimondii

mRNA and Protein(s)

mRNA	Protein	Name
XM_012594269.1	XP_012449723.1	DNA-directed RNA polymerase II subunit RPB2
XM_012594268.1	XP_012449722.1	DNA-directed RNA polymerase II subunit RPB2

Pathways from BioSystems
Gene Ontology

The following LOC105772811 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105772811 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OGo189745	XM_012594269.1 Latest version!	Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGo189745	XM_012594268.1 Latest version!	Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105772811 ( XM_012594269.1 ) cDNA ORF clone, Gossypium raimondii -> XP_012449723.1 Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGo189745
Clone ID Related Accession (Same CDS sequence)	XM_012594269.1 , XM_012594268.1
Accession Version	XM_012594269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-21
Organism	Gossypium raimondii
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012160877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gossypium raimondii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG ACAGTGAGTA CGATCCACAG CTAATGGAAG ATGAAGACGA CGAGGAGATC 
ACGCAGGAAG ATGCGTGGGC GGTTATCTCT GCTTACTTCG AAGAGAAAGG TTTGGTGCGT 
CAACAGCTCG ACTCGTTCGA TGAGTTTATC CAAAACACGA TGCAGGAAAT TGTGGATGAA 
TCAGCTGATA TTGAGATCAG GCCCGAGTCA CAGCATAATC CTGGTCATCA GTCTGACTTC 
GCTGAGACTA TCTATAAGAT TAGCTTTGGT CAGATCTATC TAAGTAAGCC TATGATGACT 
GAGTCGGATG GTGAAACTGC TACTTTATTT CCAAAAGCTG CAAGGTTGCG GAATCTCACT 
TACTCTGCTC CATTGTATGT CGATGTAACT AAAAGAGTTA TTAAAAAAGG GCATGATGGT 
GAAGAAGTCA CTGAGACTCA AGATTTTACC AAAGTGTTCA TCGGAAAGGT TCCTATAATG 
CTTCGTTCGA GTTATTGCAC ACTATATCAA AATTCAGAGA AGGATCTGAC TGAGCTTGGG 
GAGTGTCCAT ATGATCAAGG TGGGTATTTC ATTATCAATG GGAGTGAAAA AGTTCTAATT 
GCCCAAGAGA AGATGAGCAC AAATCATGTC TATGTATTCA AAAAGAGGCA ACCAAACAAA 
TACGCCTATG TAGCAGAAGT TCGATCCATG GCAGAGTCCC AAAATAGGCC ACCAAGTACC 
ATGTTTGTGC GGATGCTTTC GCGGACGAGT GCCAAAGGGG GCTCTTCAGG CCAGTACATT 
CGTGCTACTC TTCCATATAT TCGTACTGAA ATTCCTATCA TAATTGTGTT CCGGGCTTTG 
GGATTTGTTG CTGACAAGGA TATATTAGAG CATATATGCT ATGACTTCTC TGATACCCAG 
ATGATGGAGC TGCTTCGGCC TTCCTTAGAA GAAGCATTTG TGATTCAAAA CCAGCAGGTT 
GCACTAGATT ATATCGGTAA AAGAGGAGCA ACTGTTGGTG TTACCAGAGA AAAGAGGATT 
AAGTACGCAA AAGAGATCCT TCAAAAGGAA ATGCTTCCTC ACGTTGGTGT TGGAGAGTTT 
TGCGAGACAA AGAAAGCTTA TTATTTTGGG TATATCATTC ACCGGCTGCT TCTTTGTGCA 
CTTGGCCGAA GGGCAGAAGA TGATAGAGAT CATTATGGCA ACAAGAGGCT GGACCTTGCT 
GGTCCTTTAC TTGGAGGCCT CTTTAGGATG CTTTTTCGGA AGTTAACTAG GGATGTGAGA 
TCTTATGTGC AGAAGTGTGT GGATAACGGA AAGGATGTAA ACCTGCAATT TGCTATCAAA 
GCAAAAACTA TTACAAGTGG TCTTAAATAC TCACTCGCTA CTGGAAATTG GGGGCAAGCA 
AATGCAGCTG GTACAAGAGC TGGAGTGTCA CAGGTGTTAA ATCGTTTGAC ATATGCCTCC 
ACGTTGTCGC ACTTGCGAAG GCTCAATTCT CCTATAGGAC GTGAAGGGAA ATTGGCTAAG 
CCACGTCAGT TGCATAACTC ACAGTGGGGA ATGATGTGTC CAGCTGAAAC ACCAGAAGGA 
CAGGCCTGTG GACTTGTAAA GAATCTTGCC TTGATGGTGT ACATAACTGT TGGATCAGCT 
GCATATCCTA TTCTTGAATT TTTGGAAGAA TGGGGTACAG AGAATTTTGA GGAAATTTCA 
CCTGCAGTTA TTCCTCAAGC TACGAAAATT TTTGTCAATG GTTGTTGGGT TGGTGTGCAT 
CGGAATCCTG ATATGCTTGT AACCACATTG AGACGGCTGA GAAGACGGGT TGATGTCAAC 
ACTGAAGTTG GTGTTGTTCG AGATATTCGT TTGAAAGAAC TTAGAATATA TACTGACTAT 
GGCCGTTGCA GTCGGCCATT GTTCATTGTG GAGAAGCAAA GGCTTCTAAT AAAAAAGAAA 
GACATCCATG CATTGCAACA AAGGGAGACC CCAGAAGACG GTGGCTGGCA TGATCTTGTA 
GCCAAGGGAT TTATTGAATA TATTGACACA GAAGAAGAGG AGACAACTAT GATTTCCATG 
ACCATCAATG ATCTTGTCCA AGCAAGAATC AACCCAGAGG AAGCTTATTC TGAAACTTAC 
ACCCACTGTG AAATCCACCC TTCACTGATT TTGGGTGTTT GTGCTTCAAT TATACCATTC 
CCTGATCATA ATCAGTCCCC ACGTAATACC TATCAATCTG CTATGGGTAA GCAAGCAATG 
GGTATATATG TCACCAACTA CCAATTTCGA ATGGATACAT TGGCCTACGT TCTCTACTAT 
CCCCAAAAGC CACTTGTTAC TACACGAGCT ATGGAACATC TCCATTTTCG GCAGCTTCCT 
GCTGGCATTA ATGCTATTGT GGCCATTGCC TGCTATTCTG GATATAACCA AGAAGATTCC 
GTTATCATGA ATCAATCATC AATAGACCGT GGATTCTTCC GATCACTTTT CTTCCGTTCT 
TACCGGGATG AGGAGAAAAA AATGGGGACC CTCGTCAAAG AAGATTTCGG CCGACCAGAT 
AGGGCTACCA CCATGGGAAT GAGGCATGGC TCTTACGATA AATTGGATGA TGATGGTCTT 
GCACCTCCTG GAACAAGAGT TTCGGGTGAG GATGTAATTA TTGGGAAGAC CACACCTATT 
TCACAGGAGG AAGCTCAGGG TCAAGCATCA CGCTATTCAA AACGTGATCA TAGCATAAGC 
TTACGACACA GTGAGACAGG CATTGTGGAC CAGGTTCTAT TGACAACTAA TGCTGATGGA 
TTGAGATTTG TGAAAGTAAG AGTAAGATCC GTTCGGATTC CTCAGATTGG AGATAAGTTC 
AGTAGTCGGC ATGGTCAAAA GGGAACGGTT GGCATGACTT ACACCCAGGA AGACATGCCT 
TGGACTGTGG AAGGCATCAC ACCTGATATC ATTGTGAATC CACATGCTAT TCCTTCCCGT 
ATGACAATCG GTCAGCTTAT TGAGTGTATT ATGGGAAAAG TTGCCGCTCA CATGGGCAAG 
GAAGGTGATG CAACTCCATT TACAGATGTC ACTGTGGACA ATATCAGTAG AGCACTTCAT 
AAATGTGGGT ATCAAATGCG TGGTTTCGAG ACCATGTATA ATGGGCACAC AGGCAGGCGG 
CTTACTGCTA TGATATTCTT GGGACCCACA TATTACCAAC GACTAAAGCA TATGGTCGAC 
GATAAGATCC ATTCACGTGG TCGGGGTCCT GTTCAAATTC TAACGAGGCA ACCTGCAGAG 
GGACGTTCTC GTGATGGTGG TCTTCGTTTT GGAGAGATGG AACGAGATTG CATGATTGCA 
CATGGTGCTG CCCATTTCCT CAAGGAAAGA TTATTTGACC AAAGTGATGC GTACAGAGTC 
CACGTTTGTG AACGATGTGG ATTGATTGCT ATCGCGAATC TAAAAAAGAA CTCGTTTGAA 
TGCAGAGGAT GCAAGAATAA AACTGATATA GTTCAGGTTC ATATTCCTTA CGCTTGTAAA 
CTGCTGTTCC AAGAGCTTAT GGCAATGGCA ATCGCTCCAA GAATGCTTAC GAAGGAACCG 
CCCAAAGACC AAAAGAAGAA AGGAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012449723.1
CDS	131..3700
Translation	MEDDSEYDPQLMEDEDDEEITQEDAWAVISAYFEEKGLVRQQLDSFDEFIQNTMQEIVDESADIEIRPESQHNPGHQSDFAETIYKISFGQIYLSKPMMTESDGETATLFPKAARLRNLTYSAPLYVDVTKRVIKKGHDGEEVTETQDFTKVFIGKVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVAEVRSMAESQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRALGFVADKDILEHICYDFSDTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTREKRIKYAKEILQKEMLPHVGVGEFCETKKAYYFGYIIHRLLLCALGRRAEDDRDHYGNKRLDLAGPLLGGLFRMLFRKLTRDVRSYVQKCVDNGKDVNLQFAIKAKTITSGLKYSLATGNWGQANAAGTRAGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPAETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPAVIPQATKIFVNGCWVGVHRNPDMLVTTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRPLFIVEKQRLLIKKKDIHALQQRETPEDGGWHDLVAKGFIEYIDTEEEETTMISMTINDLVQARINPEEAYSETYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGIYVTNYQFRMDTLAYVLYYPQKPLVTTRAMEHLHFRQLPAGINAIVAIACYSGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPDRATTMGMRHGSYDKLDDDGLAPPGTRVSGEDVIIGKTTPISQEEAQGQASRYSKRDHSISLRHSETGIVDQVLLTTNADGLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTVEGITPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISRALHKCGYQMRGFETMYNGHTGRRLTAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLRFGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCERCGLIAIANLKKNSFECRGCKNKTDIVQVHIPYACKLLFQELMAMAIAPRMLTKEPPKDQKKKGA

Target ORF information:

RefSeq Version	XM_012594269.1
Organism	Gossypium raimondii
Definition	Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012594269.1

ORF Insert Sequence:

ATGGAGGACG ACAGTGAGTA CGATCCACAG CTAATGGAAG ATGAAGACGA CGAGGAGATC 
ACGCAGGAAG ATGCGTGGGC GGTTATCTCT GCTTACTTCG AAGAGAAAGG TTTGGTGCGT 
CAACAGCTCG ACTCGTTCGA TGAGTTTATC CAAAACACGA TGCAGGAAAT TGTGGATGAA 
TCAGCTGATA TTGAGATCAG GCCCGAGTCA CAGCATAATC CTGGTCATCA GTCTGACTTC 
GCTGAGACTA TCTATAAGAT TAGCTTTGGT CAGATCTATC TAAGTAAGCC TATGATGACT 
GAGTCGGATG GTGAAACTGC TACTTTATTT CCAAAAGCTG CAAGGTTGCG GAATCTCACT 
TACTCTGCTC CATTGTATGT CGATGTAACT AAAAGAGTTA TTAAAAAAGG GCATGATGGT 
GAAGAAGTCA CTGAGACTCA AGATTTTACC AAAGTGTTCA TCGGAAAGGT TCCTATAATG 
CTTCGTTCGA GTTATTGCAC ACTATATCAA AATTCAGAGA AGGATCTGAC TGAGCTTGGG 
GAGTGTCCAT ATGATCAAGG TGGGTATTTC ATTATCAATG GGAGTGAAAA AGTTCTAATT 
GCCCAAGAGA AGATGAGCAC AAATCATGTC TATGTATTCA AAAAGAGGCA ACCAAACAAA 
TACGCCTATG TAGCAGAAGT TCGATCCATG GCAGAGTCCC AAAATAGGCC ACCAAGTACC 
ATGTTTGTGC GGATGCTTTC GCGGACGAGT GCCAAAGGGG GCTCTTCAGG CCAGTACATT 
CGTGCTACTC TTCCATATAT TCGTACTGAA ATTCCTATCA TAATTGTGTT CCGGGCTTTG 
GGATTTGTTG CTGACAAGGA TATATTAGAG CATATATGCT ATGACTTCTC TGATACCCAG 
ATGATGGAGC TGCTTCGGCC TTCCTTAGAA GAAGCATTTG TGATTCAAAA CCAGCAGGTT 
GCACTAGATT ATATCGGTAA AAGAGGAGCA ACTGTTGGTG TTACCAGAGA AAAGAGGATT 
AAGTACGCAA AAGAGATCCT TCAAAAGGAA ATGCTTCCTC ACGTTGGTGT TGGAGAGTTT 
TGCGAGACAA AGAAAGCTTA TTATTTTGGG TATATCATTC ACCGGCTGCT TCTTTGTGCA 
CTTGGCCGAA GGGCAGAAGA TGATAGAGAT CATTATGGCA ACAAGAGGCT GGACCTTGCT 
GGTCCTTTAC TTGGAGGCCT CTTTAGGATG CTTTTTCGGA AGTTAACTAG GGATGTGAGA 
TCTTATGTGC AGAAGTGTGT GGATAACGGA AAGGATGTAA ACCTGCAATT TGCTATCAAA 
GCAAAAACTA TTACAAGTGG TCTTAAATAC TCACTCGCTA CTGGAAATTG GGGGCAAGCA 
AATGCAGCTG GTACAAGAGC TGGAGTGTCA CAGGTGTTAA ATCGTTTGAC ATATGCCTCC 
ACGTTGTCGC ACTTGCGAAG GCTCAATTCT CCTATAGGAC GTGAAGGGAA ATTGGCTAAG 
CCACGTCAGT TGCATAACTC ACAGTGGGGA ATGATGTGTC CAGCTGAAAC ACCAGAAGGA 
CAGGCCTGTG GACTTGTAAA GAATCTTGCC TTGATGGTGT ACATAACTGT TGGATCAGCT 
GCATATCCTA TTCTTGAATT TTTGGAAGAA TGGGGTACAG AGAATTTTGA GGAAATTTCA 
CCTGCAGTTA TTCCTCAAGC TACGAAAATT TTTGTCAATG GTTGTTGGGT TGGTGTGCAT 
CGGAATCCTG ATATGCTTGT AACCACATTG AGACGGCTGA GAAGACGGGT TGATGTCAAC 
ACTGAAGTTG GTGTTGTTCG AGATATTCGT TTGAAAGAAC TTAGAATATA TACTGACTAT 
GGCCGTTGCA GTCGGCCATT GTTCATTGTG GAGAAGCAAA GGCTTCTAAT AAAAAAGAAA 
GACATCCATG CATTGCAACA AAGGGAGACC CCAGAAGACG GTGGCTGGCA TGATCTTGTA 
GCCAAGGGAT TTATTGAATA TATTGACACA GAAGAAGAGG AGACAACTAT GATTTCCATG 
ACCATCAATG ATCTTGTCCA AGCAAGAATC AACCCAGAGG AAGCTTATTC TGAAACTTAC 
ACCCACTGTG AAATCCACCC TTCACTGATT TTGGGTGTTT GTGCTTCAAT TATACCATTC 
CCTGATCATA ATCAGTCCCC ACGTAATACC TATCAATCTG CTATGGGTAA GCAAGCAATG 
GGTATATATG TCACCAACTA CCAATTTCGA ATGGATACAT TGGCCTACGT TCTCTACTAT 
CCCCAAAAGC CACTTGTTAC TACACGAGCT ATGGAACATC TCCATTTTCG GCAGCTTCCT 
GCTGGCATTA ATGCTATTGT GGCCATTGCC TGCTATTCTG GATATAACCA AGAAGATTCC 
GTTATCATGA ATCAATCATC AATAGACCGT GGATTCTTCC GATCACTTTT CTTCCGTTCT 
TACCGGGATG AGGAGAAAAA AATGGGGACC CTCGTCAAAG AAGATTTCGG CCGACCAGAT 
AGGGCTACCA CCATGGGAAT GAGGCATGGC TCTTACGATA AATTGGATGA TGATGGTCTT 
GCACCTCCTG GAACAAGAGT TTCGGGTGAG GATGTAATTA TTGGGAAGAC CACACCTATT 
TCACAGGAGG AAGCTCAGGG TCAAGCATCA CGCTATTCAA AACGTGATCA TAGCATAAGC 
TTACGACACA GTGAGACAGG CATTGTGGAC CAGGTTCTAT TGACAACTAA TGCTGATGGA 
TTGAGATTTG TGAAAGTAAG AGTAAGATCC GTTCGGATTC CTCAGATTGG AGATAAGTTC 
AGTAGTCGGC ATGGTCAAAA GGGAACGGTT GGCATGACTT ACACCCAGGA AGACATGCCT 
TGGACTGTGG AAGGCATCAC ACCTGATATC ATTGTGAATC CACATGCTAT TCCTTCCCGT 
ATGACAATCG GTCAGCTTAT TGAGTGTATT ATGGGAAAAG TTGCCGCTCA CATGGGCAAG 
GAAGGTGATG CAACTCCATT TACAGATGTC ACTGTGGACA ATATCAGTAG AGCACTTCAT 
AAATGTGGGT ATCAAATGCG TGGTTTCGAG ACCATGTATA ATGGGCACAC AGGCAGGCGG 
CTTACTGCTA TGATATTCTT GGGACCCACA TATTACCAAC GACTAAAGCA TATGGTCGAC 
GATAAGATCC ATTCACGTGG TCGGGGTCCT GTTCAAATTC TAACGAGGCA ACCTGCAGAG 
GGACGTTCTC GTGATGGTGG TCTTCGTTTT GGAGAGATGG AACGAGATTG CATGATTGCA 
CATGGTGCTG CCCATTTCCT CAAGGAAAGA TTATTTGACC AAAGTGATGC GTACAGAGTC 
CACGTTTGTG AACGATGTGG ATTGATTGCT ATCGCGAATC TAAAAAAGAA CTCGTTTGAA 
TGCAGAGGAT GCAAGAATAA AACTGATATA GTTCAGGTTC ATATTCCTTA CGCTTGTAAA 
CTGCTGTTCC AAGAGCTTAT GGCAATGGCA ATCGCTCCAA GAATGCTTAC GAAGGAACCG 
CCCAAAGACC AAAAGAAGAA AGGAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105772811 ( XM_012594268.1 ) cDNA ORF clone, Gossypium raimondii -> XP_012449722.1 Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGo189745
Clone ID Related Accession (Same CDS sequence)	XM_012594269.1 , XM_012594268.1
Accession Version	XM_012594268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3570bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-21
Organism	Gossypium raimondii
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012160877.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gossypium raimondii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG ACAGTGAGTA CGATCCACAG CTAATGGAAG ATGAAGACGA CGAGGAGATC 
ACGCAGGAAG ATGCGTGGGC GGTTATCTCT GCTTACTTCG AAGAGAAAGG TTTGGTGCGT 
CAACAGCTCG ACTCGTTCGA TGAGTTTATC CAAAACACGA TGCAGGAAAT TGTGGATGAA 
TCAGCTGATA TTGAGATCAG GCCCGAGTCA CAGCATAATC CTGGTCATCA GTCTGACTTC 
GCTGAGACTA TCTATAAGAT TAGCTTTGGT CAGATCTATC TAAGTAAGCC TATGATGACT 
GAGTCGGATG GTGAAACTGC TACTTTATTT CCAAAAGCTG CAAGGTTGCG GAATCTCACT 
TACTCTGCTC CATTGTATGT CGATGTAACT AAAAGAGTTA TTAAAAAAGG GCATGATGGT 
GAAGAAGTCA CTGAGACTCA AGATTTTACC AAAGTGTTCA TCGGAAAGGT TCCTATAATG 
CTTCGTTCGA GTTATTGCAC ACTATATCAA AATTCAGAGA AGGATCTGAC TGAGCTTGGG 
GAGTGTCCAT ATGATCAAGG TGGGTATTTC ATTATCAATG GGAGTGAAAA AGTTCTAATT 
GCCCAAGAGA AGATGAGCAC AAATCATGTC TATGTATTCA AAAAGAGGCA ACCAAACAAA 
TACGCCTATG TAGCAGAAGT TCGATCCATG GCAGAGTCCC AAAATAGGCC ACCAAGTACC 
ATGTTTGTGC GGATGCTTTC GCGGACGAGT GCCAAAGGGG GCTCTTCAGG CCAGTACATT 
CGTGCTACTC TTCCATATAT TCGTACTGAA ATTCCTATCA TAATTGTGTT CCGGGCTTTG 
GGATTTGTTG CTGACAAGGA TATATTAGAG CATATATGCT ATGACTTCTC TGATACCCAG 
ATGATGGAGC TGCTTCGGCC TTCCTTAGAA GAAGCATTTG TGATTCAAAA CCAGCAGGTT 
GCACTAGATT ATATCGGTAA AAGAGGAGCA ACTGTTGGTG TTACCAGAGA AAAGAGGATT 
AAGTACGCAA AAGAGATCCT TCAAAAGGAA ATGCTTCCTC ACGTTGGTGT TGGAGAGTTT 
TGCGAGACAA AGAAAGCTTA TTATTTTGGG TATATCATTC ACCGGCTGCT TCTTTGTGCA 
CTTGGCCGAA GGGCAGAAGA TGATAGAGAT CATTATGGCA ACAAGAGGCT GGACCTTGCT 
GGTCCTTTAC TTGGAGGCCT CTTTAGGATG CTTTTTCGGA AGTTAACTAG GGATGTGAGA 
TCTTATGTGC AGAAGTGTGT GGATAACGGA AAGGATGTAA ACCTGCAATT TGCTATCAAA 
GCAAAAACTA TTACAAGTGG TCTTAAATAC TCACTCGCTA CTGGAAATTG GGGGCAAGCA 
AATGCAGCTG GTACAAGAGC TGGAGTGTCA CAGGTGTTAA ATCGTTTGAC ATATGCCTCC 
ACGTTGTCGC ACTTGCGAAG GCTCAATTCT CCTATAGGAC GTGAAGGGAA ATTGGCTAAG 
CCACGTCAGT TGCATAACTC ACAGTGGGGA ATGATGTGTC CAGCTGAAAC ACCAGAAGGA 
CAGGCCTGTG GACTTGTAAA GAATCTTGCC TTGATGGTGT ACATAACTGT TGGATCAGCT 
GCATATCCTA TTCTTGAATT TTTGGAAGAA TGGGGTACAG AGAATTTTGA GGAAATTTCA 
CCTGCAGTTA TTCCTCAAGC TACGAAAATT TTTGTCAATG GTTGTTGGGT TGGTGTGCAT 
CGGAATCCTG ATATGCTTGT AACCACATTG AGACGGCTGA GAAGACGGGT TGATGTCAAC 
ACTGAAGTTG GTGTTGTTCG AGATATTCGT TTGAAAGAAC TTAGAATATA TACTGACTAT 
GGCCGTTGCA GTCGGCCATT GTTCATTGTG GAGAAGCAAA GGCTTCTAAT AAAAAAGAAA 
GACATCCATG CATTGCAACA AAGGGAGACC CCAGAAGACG GTGGCTGGCA TGATCTTGTA 
GCCAAGGGAT TTATTGAATA TATTGACACA GAAGAAGAGG AGACAACTAT GATTTCCATG 
ACCATCAATG ATCTTGTCCA AGCAAGAATC AACCCAGAGG AAGCTTATTC TGAAACTTAC 
ACCCACTGTG AAATCCACCC TTCACTGATT TTGGGTGTTT GTGCTTCAAT TATACCATTC 
CCTGATCATA ATCAGTCCCC ACGTAATACC TATCAATCTG CTATGGGTAA GCAAGCAATG 
GGTATATATG TCACCAACTA CCAATTTCGA ATGGATACAT TGGCCTACGT TCTCTACTAT 
CCCCAAAAGC CACTTGTTAC TACACGAGCT ATGGAACATC TCCATTTTCG GCAGCTTCCT 
GCTGGCATTA ATGCTATTGT GGCCATTGCC TGCTATTCTG GATATAACCA AGAAGATTCC 
GTTATCATGA ATCAATCATC AATAGACCGT GGATTCTTCC GATCACTTTT CTTCCGTTCT 
TACCGGGATG AGGAGAAAAA AATGGGGACC CTCGTCAAAG AAGATTTCGG CCGACCAGAT 
AGGGCTACCA CCATGGGAAT GAGGCATGGC TCTTACGATA AATTGGATGA TGATGGTCTT 
GCACCTCCTG GAACAAGAGT TTCGGGTGAG GATGTAATTA TTGGGAAGAC CACACCTATT 
TCACAGGAGG AAGCTCAGGG TCAAGCATCA CGCTATTCAA AACGTGATCA TAGCATAAGC 
TTACGACACA GTGAGACAGG CATTGTGGAC CAGGTTCTAT TGACAACTAA TGCTGATGGA 
TTGAGATTTG TGAAAGTAAG AGTAAGATCC GTTCGGATTC CTCAGATTGG AGATAAGTTC 
AGTAGTCGGC ATGGTCAAAA GGGAACGGTT GGCATGACTT ACACCCAGGA AGACATGCCT 
TGGACTGTGG AAGGCATCAC ACCTGATATC ATTGTGAATC CACATGCTAT TCCTTCCCGT 
ATGACAATCG GTCAGCTTAT TGAGTGTATT ATGGGAAAAG TTGCCGCTCA CATGGGCAAG 
GAAGGTGATG CAACTCCATT TACAGATGTC ACTGTGGACA ATATCAGTAG AGCACTTCAT 
AAATGTGGGT ATCAAATGCG TGGTTTCGAG ACCATGTATA ATGGGCACAC AGGCAGGCGG 
CTTACTGCTA TGATATTCTT GGGACCCACA TATTACCAAC GACTAAAGCA TATGGTCGAC 
GATAAGATCC ATTCACGTGG TCGGGGTCCT GTTCAAATTC TAACGAGGCA ACCTGCAGAG 
GGACGTTCTC GTGATGGTGG TCTTCGTTTT GGAGAGATGG AACGAGATTG CATGATTGCA 
CATGGTGCTG CCCATTTCCT CAAGGAAAGA TTATTTGACC AAAGTGATGC GTACAGAGTC 
CACGTTTGTG AACGATGTGG ATTGATTGCT ATCGCGAATC TAAAAAAGAA CTCGTTTGAA 
TGCAGAGGAT GCAAGAATAA AACTGATATA GTTCAGGTTC ATATTCCTTA CGCTTGTAAA 
CTGCTGTTCC AAGAGCTTAT GGCAATGGCA ATCGCTCCAA GAATGCTTAC GAAGGAACCG 
CCCAAAGACC AAAAGAAGAA AGGAGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012449722.1
CDS	131..3700
Translation	MEDDSEYDPQLMEDEDDEEITQEDAWAVISAYFEEKGLVRQQLDSFDEFIQNTMQEIVDESADIEIRPESQHNPGHQSDFAETIYKISFGQIYLSKPMMTESDGETATLFPKAARLRNLTYSAPLYVDVTKRVIKKGHDGEEVTETQDFTKVFIGKVPIMLRSSYCTLYQNSEKDLTELGECPYDQGGYFIINGSEKVLIAQEKMSTNHVYVFKKRQPNKYAYVAEVRSMAESQNRPPSTMFVRMLSRTSAKGGSSGQYIRATLPYIRTEIPIIIVFRALGFVADKDILEHICYDFSDTQMMELLRPSLEEAFVIQNQQVALDYIGKRGATVGVTREKRIKYAKEILQKEMLPHVGVGEFCETKKAYYFGYIIHRLLLCALGRRAEDDRDHYGNKRLDLAGPLLGGLFRMLFRKLTRDVRSYVQKCVDNGKDVNLQFAIKAKTITSGLKYSLATGNWGQANAAGTRAGVSQVLNRLTYASTLSHLRRLNSPIGREGKLAKPRQLHNSQWGMMCPAETPEGQACGLVKNLALMVYITVGSAAYPILEFLEEWGTENFEEISPAVIPQATKIFVNGCWVGVHRNPDMLVTTLRRLRRRVDVNTEVGVVRDIRLKELRIYTDYGRCSRPLFIVEKQRLLIKKKDIHALQQRETPEDGGWHDLVAKGFIEYIDTEEEETTMISMTINDLVQARINPEEAYSETYTHCEIHPSLILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGIYVTNYQFRMDTLAYVLYYPQKPLVTTRAMEHLHFRQLPAGINAIVAIACYSGYNQEDSVIMNQSSIDRGFFRSLFFRSYRDEEKKMGTLVKEDFGRPDRATTMGMRHGSYDKLDDDGLAPPGTRVSGEDVIIGKTTPISQEEAQGQASRYSKRDHSISLRHSETGIVDQVLLTTNADGLRFVKVRVRSVRIPQIGDKFSSRHGQKGTVGMTYTQEDMPWTVEGITPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTDVTVDNISRALHKCGYQMRGFETMYNGHTGRRLTAMIFLGPTYYQRLKHMVDDKIHSRGRGPVQILTRQPAEGRSRDGGLRFGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCERCGLIAIANLKKNSFECRGCKNKTDIVQVHIPYACKLLFQELMAMAIAPRMLTKEPPKDQKKKGA

Target ORF information:

RefSeq Version	XM_012594268.1
Organism	Gossypium raimondii
Definition	Gossypium raimondii DNA-directed RNA polymerase II subunit RPB2 (LOC105772811), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012594268.1

ORF Insert Sequence:

ATGGAGGACG ACAGTGAGTA CGATCCACAG CTAATGGAAG ATGAAGACGA CGAGGAGATC 
ACGCAGGAAG ATGCGTGGGC GGTTATCTCT GCTTACTTCG AAGAGAAAGG TTTGGTGCGT 
CAACAGCTCG ACTCGTTCGA TGAGTTTATC CAAAACACGA TGCAGGAAAT TGTGGATGAA 
TCAGCTGATA TTGAGATCAG GCCCGAGTCA CAGCATAATC CTGGTCATCA GTCTGACTTC 
GCTGAGACTA TCTATAAGAT TAGCTTTGGT CAGATCTATC TAAGTAAGCC TATGATGACT 
GAGTCGGATG GTGAAACTGC TACTTTATTT CCAAAAGCTG CAAGGTTGCG GAATCTCACT 
TACTCTGCTC CATTGTATGT CGATGTAACT AAAAGAGTTA TTAAAAAAGG GCATGATGGT 
GAAGAAGTCA CTGAGACTCA AGATTTTACC AAAGTGTTCA TCGGAAAGGT TCCTATAATG 
CTTCGTTCGA GTTATTGCAC ACTATATCAA AATTCAGAGA AGGATCTGAC TGAGCTTGGG 
GAGTGTCCAT ATGATCAAGG TGGGTATTTC ATTATCAATG GGAGTGAAAA AGTTCTAATT 
GCCCAAGAGA AGATGAGCAC AAATCATGTC TATGTATTCA AAAAGAGGCA ACCAAACAAA 
TACGCCTATG TAGCAGAAGT TCGATCCATG GCAGAGTCCC AAAATAGGCC ACCAAGTACC 
ATGTTTGTGC GGATGCTTTC GCGGACGAGT GCCAAAGGGG GCTCTTCAGG CCAGTACATT 
CGTGCTACTC TTCCATATAT TCGTACTGAA ATTCCTATCA TAATTGTGTT CCGGGCTTTG 
GGATTTGTTG CTGACAAGGA TATATTAGAG CATATATGCT ATGACTTCTC TGATACCCAG 
ATGATGGAGC TGCTTCGGCC TTCCTTAGAA GAAGCATTTG TGATTCAAAA CCAGCAGGTT 
GCACTAGATT ATATCGGTAA AAGAGGAGCA ACTGTTGGTG TTACCAGAGA AAAGAGGATT 
AAGTACGCAA AAGAGATCCT TCAAAAGGAA ATGCTTCCTC ACGTTGGTGT TGGAGAGTTT 
TGCGAGACAA AGAAAGCTTA TTATTTTGGG TATATCATTC ACCGGCTGCT TCTTTGTGCA 
CTTGGCCGAA GGGCAGAAGA TGATAGAGAT CATTATGGCA ACAAGAGGCT GGACCTTGCT 
GGTCCTTTAC TTGGAGGCCT CTTTAGGATG CTTTTTCGGA AGTTAACTAG GGATGTGAGA 
TCTTATGTGC AGAAGTGTGT GGATAACGGA AAGGATGTAA ACCTGCAATT TGCTATCAAA 
GCAAAAACTA TTACAAGTGG TCTTAAATAC TCACTCGCTA CTGGAAATTG GGGGCAAGCA 
AATGCAGCTG GTACAAGAGC TGGAGTGTCA CAGGTGTTAA ATCGTTTGAC ATATGCCTCC 
ACGTTGTCGC ACTTGCGAAG GCTCAATTCT CCTATAGGAC GTGAAGGGAA ATTGGCTAAG 
CCACGTCAGT TGCATAACTC ACAGTGGGGA ATGATGTGTC CAGCTGAAAC ACCAGAAGGA 
CAGGCCTGTG GACTTGTAAA GAATCTTGCC TTGATGGTGT ACATAACTGT TGGATCAGCT 
GCATATCCTA TTCTTGAATT TTTGGAAGAA TGGGGTACAG AGAATTTTGA GGAAATTTCA 
CCTGCAGTTA TTCCTCAAGC TACGAAAATT TTTGTCAATG GTTGTTGGGT TGGTGTGCAT 
CGGAATCCTG ATATGCTTGT AACCACATTG AGACGGCTGA GAAGACGGGT TGATGTCAAC 
ACTGAAGTTG GTGTTGTTCG AGATATTCGT TTGAAAGAAC TTAGAATATA TACTGACTAT 
GGCCGTTGCA GTCGGCCATT GTTCATTGTG GAGAAGCAAA GGCTTCTAAT AAAAAAGAAA 
GACATCCATG CATTGCAACA AAGGGAGACC CCAGAAGACG GTGGCTGGCA TGATCTTGTA 
GCCAAGGGAT TTATTGAATA TATTGACACA GAAGAAGAGG AGACAACTAT GATTTCCATG 
ACCATCAATG ATCTTGTCCA AGCAAGAATC AACCCAGAGG AAGCTTATTC TGAAACTTAC 
ACCCACTGTG AAATCCACCC TTCACTGATT TTGGGTGTTT GTGCTTCAAT TATACCATTC 
CCTGATCATA ATCAGTCCCC ACGTAATACC TATCAATCTG CTATGGGTAA GCAAGCAATG 
GGTATATATG TCACCAACTA CCAATTTCGA ATGGATACAT TGGCCTACGT TCTCTACTAT 
CCCCAAAAGC CACTTGTTAC TACACGAGCT ATGGAACATC TCCATTTTCG GCAGCTTCCT 
GCTGGCATTA ATGCTATTGT GGCCATTGCC TGCTATTCTG GATATAACCA AGAAGATTCC 
GTTATCATGA ATCAATCATC AATAGACCGT GGATTCTTCC GATCACTTTT CTTCCGTTCT 
TACCGGGATG AGGAGAAAAA AATGGGGACC CTCGTCAAAG AAGATTTCGG CCGACCAGAT 
AGGGCTACCA CCATGGGAAT GAGGCATGGC TCTTACGATA AATTGGATGA TGATGGTCTT 
GCACCTCCTG GAACAAGAGT TTCGGGTGAG GATGTAATTA TTGGGAAGAC CACACCTATT 
TCACAGGAGG AAGCTCAGGG TCAAGCATCA CGCTATTCAA AACGTGATCA TAGCATAAGC 
TTACGACACA GTGAGACAGG CATTGTGGAC CAGGTTCTAT TGACAACTAA TGCTGATGGA 
TTGAGATTTG TGAAAGTAAG AGTAAGATCC GTTCGGATTC CTCAGATTGG AGATAAGTTC 
AGTAGTCGGC ATGGTCAAAA GGGAACGGTT GGCATGACTT ACACCCAGGA AGACATGCCT 
TGGACTGTGG AAGGCATCAC ACCTGATATC ATTGTGAATC CACATGCTAT TCCTTCCCGT 
ATGACAATCG GTCAGCTTAT TGAGTGTATT ATGGGAAAAG TTGCCGCTCA CATGGGCAAG 
GAAGGTGATG CAACTCCATT TACAGATGTC ACTGTGGACA ATATCAGTAG AGCACTTCAT 
AAATGTGGGT ATCAAATGCG TGGTTTCGAG ACCATGTATA ATGGGCACAC AGGCAGGCGG 
CTTACTGCTA TGATATTCTT GGGACCCACA TATTACCAAC GACTAAAGCA TATGGTCGAC 
GATAAGATCC ATTCACGTGG TCGGGGTCCT GTTCAAATTC TAACGAGGCA ACCTGCAGAG 
GGACGTTCTC GTGATGGTGG TCTTCGTTTT GGAGAGATGG AACGAGATTG CATGATTGCA 
CATGGTGCTG CCCATTTCCT CAAGGAAAGA TTATTTGACC AAAGTGATGC GTACAGAGTC 
CACGTTTGTG AACGATGTGG ATTGATTGCT ATCGCGAATC TAAAAAAGAA CTCGTTTGAA 
TGCAGAGGAT GCAAGAATAA AACTGATATA GTTCAGGTTC ATATTCCTTA CGCTTGTAAA 
CTGCTGTTCC AAGAGCTTAT GGCAATGGCA ATCGCTCCAA GAATGCTTAC GAAGGAACCG 
CCCAAAGACC AAAAGAAGAA AGGAGCGTGA