LOC547944 cDNA ORF clone, Glycine max(Soybean)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC547944
Entrez Gene ID	547944
Full Name	4-coumarate--CoA ligase 1
Gene Type	protein-coding
Organism	Glycine max(Soybean)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001249489.2	NP_001236418.1	4-coumarate--CoA ligase 2
NM_001249489.1	NP_001236418.1	4-coumarate--CoA ligase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Differential expression of isoflavone biosynthetic genes in soybean during water deficits.
Gutierrez-Gonzalez JJ, Guttikonda SK, Tran LS, Aldrich DL, Zhong R, Yu O, Nguyen HT, Sleper DA
Plant & cell physiology51(6)936-48(2010 Jun)

Deletion of a single amino acid residue from different 4-coumarate:CoA ligases from soybean results in the generation of new substrate specificities.
Lindermayr C, Fliegmann J, Ebel J
The Journal of biological chemistry278(5)2781-6(2003 Jan)

Divergent members of a soybean (Glycine max L.) 4-coumarate:coenzyme A ligase gene family.
Lindermayr C, M?llers B, Fliegmann J, Uhlmann A, Lottspeich F, Meimberg H, Ebel J
European journal of biochemistry269(4)1304-15(2002 Feb)

Molecular cloning and expression of 4-coumarate:coenzyme A ligase, an enzyme involved in the resistance response of soybean (Glycine max L.) against pathogen attack.
Uhlmann A, Ebel J
Plant physiology102(4)1147-56(1993 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC547944 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC547944 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGl54226	NM_001249489.2 Latest version!	Glycine max 4-coumarate--CoA ligase 2 (4CL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
View Old Accession Versions >>
OGl54226	NM_001249489.1	Glycine max 4-coumarate:CoA ligase isoenzyme 2 (LOC547944), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC547944 ( NM_001249489.2 ) cDNA ORF clone, Glycine max(Soybean) -> NP_001236418.1 Glycine max 4-coumarate--CoA ligase 2 (4CL2), mRNA.

Price & Availability↑

CloneID	OGl54226
Clone ID Related Accession (Same CDS sequence)	NM_001249489.2 , NM_001249489.1
Accession Version	NM_001249489.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Glycine max(soybean)
Product	4-coumarate--CoA ligase 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF002259.1 and ACUP03008496.1. On Nov 23, 2017 this sequence version replaced NM_001249489.1. ##Evidence-Data-START## Transcript exon combination :: AF002259.1, X69954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264989, SAMN00769647 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGATG ATGGAAGCAG GAGGGAATTG ATATTCAGGT CGAAGCTTCC GGATATCTAC 
ATCCCCAAAC ATATGCCCCT CCACTCTTAC TGCTTCGAGA ATTTGAGAGA GTGTGGTTCA 
CGCCCCTGCC TGATCAATGC CCCCACGGGA GACGTCTACA GCTACCACGA GGTGGACAGC 
ACCGCCAGAA AGGTGGCGAG GGGGCTGAAG AAAGAGGGCG TGGAACAGGG CCAGGTCATC 
ATGATCCTCC TCCCCAATTG CCCCGAATTC GTCTTCTCGT TTCTTGGCGC ATCCCACCGC 
GGTGCCATGG CCACTGCCGC CAACCCTTTC TTCACCCCCG CCGAGATTGC AAAGCAAGCC 
CATGCCTCCA ATGCCAAGCT CCTCATCACC CAGGCCTCTT ACTACGACAA AGTCAAGGAC 
CTCCGCGACA TCAAGCTCGT ATTCGTCGAC TCTTGTCCCC CCCATACAGA GGAGAAGCAG 
CATCTCCATT TCTCACATCT GTGTGAGGAT AACGGTGACG CTGATGTTGA TGTTGATGTT 
GATATCAAGC CCGATGACGT GGTTGCATTG CCTTATTCTT CGGGAACAAC TGGTCTTCCC 
AAGGGAGTGA TGCTGAGCCA CAAGGGGCTG GTGACCAGCA TTGCTCAGCA GGTTGACGGT 
GATAATCCCA ACCTCTATTA CCACTGCCAC GACACCATCC TCTGCGTGCT TCCCCTCTTT 
CACATTTACT CTCTCAACTC CGTTCTTCTC TGTGGCTTGC GTGCCAAGGC TACCATTCTC 
CTCATGCCCA AGTTCGATAT TAACTCCTTG CTTGCTCTCA TTCACAAGCA CAAAGTCACT 
ATTGCCCCTG TCGTCCCTCC CATTGTTCTC GCCATTTCCA AATCACCCGA TCTCCACAAG 
TACGACCTGT CTTCCATCAG AGTCTTGAAG TCCGGGGGAG CCCCTCTGGG TAAGGAACTC 
GAAGACACTC TCAGAGCTAA ATTCCCCAAC GCCAAACTTG GCCAGGGATA CGGAATGACT 
GAGGCAGGGC CTGTGCTAAC AATGTCTTTA GCTTTTGCTA AAGAACCGAT AGACGTGAAA 
CCAGGTGCAT GTGGAACCGT TGTAAGAAAT GCAGAGATGA AGATTGTCGA TCCTGAAACC 
GGTCATTCTT TACCCAGAAA CCAATCCGGT GAAATTTGCA TAAGAGGCGA CCAGATTATG 
AAAGGTTATC TAAATGATGG AGAGGCTACA GAGAGAACCA TAGACAAAGA TGGTTGGTTG 
CACACAGGTG ACATCGGTTA CATCGACGAT GACGATGAGT TATTCATCGT TGACAGGCTC 
AAGGAATTGA TCAAATACAA AGGATTTCAA GTGGCTCCAG CTGAACTTGA AGCCCTTCTT 
CTCACTCATC CTAAGATCTC TGATGCTGCT GTTGTTCCAA TGAAGGATGA AGCCGCGGGA 
GAGGTACCTG TTGCATTTGT GGTCATATCA AATGGTTATA CCGACACAAC CGAGGATGAA 
ATTAAGCAGT TTATCTCCAA ACAGGTGGTG TTTTACAAAA GAATAAACCG AGTATTCTTC 
ATTGATGCAA TTCCCAAGTC ACCGTCAGGC AAAATCTTGC GCAAGGATCT AAGAGCAAAG 
ATAGCGGCAA GTGTTCCAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001236418.1
CDS	67..1710
Translation	MADDGSRRELIFRSKLPDIYIPKHMPLHSYCFENLRECGSRPCLINAPTGDVYSYHEVDSTARKVARGLKKEGVEQGQVIMILLPNCPEFVFSFLGASHRGAMATAANPFFTPAEIAKQAHASNAKLLITQASYYDKVKDLRDIKLVFVDSCPPHTEEKQHLHFSHLCEDNGDADVDVDVDIKPDDVVALPYSSGTTGLPKGVMLSHKGLVTSIAQQVDGDNPNLYYHCHDTILCVLPLFHIYSLNSVLLCGLRAKATILLMPKFDINSLLALIHKHKVTIAPVVPPIVLAISKSPDLHKYDLSSIRVLKSGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFAKEPIDVKPGACGTVVRNAEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVPMKDEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKIAASVPK

Target ORF information:

RefSeq Version	NM_001249489.2
Organism	Glycine max(soybean)
Definition	Glycine max 4-coumarate--CoA ligase 2 (4CL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001249489.2

ORF Insert Sequence:

ATGGCTGATG ATGGAAGCAG GAGGGAATTG ATATTCAGGT CGAAGCTTCC GGATATCTAC 
ATCCCCAAAC ATATGCCCCT CCACTCTTAC TGCTTCGAGA ATTTGAGAGA GTGTGGTTCA 
CGCCCCTGCC TGATCAATGC CCCCACGGGA GACGTCTACA GCTACCACGA GGTGGACAGC 
ACCGCCAGAA AGGTGGCGAG GGGGCTGAAG AAAGAGGGCG TGGAACAGGG CCAGGTCATC 
ATGATCCTCC TCCCCAATTG CCCCGAATTC GTCTTCTCGT TTCTTGGCGC ATCCCACCGC 
GGTGCCATGG CCACTGCCGC CAACCCTTTC TTCACCCCCG CCGAGATTGC AAAGCAAGCC 
CATGCCTCCA ATGCCAAGCT CCTCATCACC CAGGCCTCTT ACTACGACAA AGTCAAGGAC 
CTCCGCGACA TCAAGCTCGT ATTCGTCGAC TCTTGTCCCC CCCATACAGA GGAGAAGCAG 
CATCTCCATT TCTCACATCT GTGTGAGGAT AACGGTGACG CTGATGTTGA TGTTGATGTT 
GATATCAAGC CCGATGACGT GGTTGCATTG CCTTATTCTT CGGGAACAAC TGGTCTTCCC 
AAGGGAGTGA TGCTGAGCCA CAAGGGGCTG GTGACCAGCA TTGCTCAGCA GGTTGACGGT 
GATAATCCCA ACCTCTATTA CCACTGCCAC GACACCATCC TCTGCGTGCT TCCCCTCTTT 
CACATTTACT CTCTCAACTC CGTTCTTCTC TGTGGCTTGC GTGCCAAGGC TACCATTCTC 
CTCATGCCCA AGTTCGATAT TAACTCCTTG CTTGCTCTCA TTCACAAGCA CAAAGTCACT 
ATTGCCCCTG TCGTCCCTCC CATTGTTCTC GCCATTTCCA AATCACCCGA TCTCCACAAG 
TACGACCTGT CTTCCATCAG AGTCTTGAAG TCCGGGGGAG CCCCTCTGGG TAAGGAACTC 
GAAGACACTC TCAGAGCTAA ATTCCCCAAC GCCAAACTTG GCCAGGGATA CGGAATGACT 
GAGGCAGGGC CTGTGCTAAC AATGTCTTTA GCTTTTGCTA AAGAACCGAT AGACGTGAAA 
CCAGGTGCAT GTGGAACCGT TGTAAGAAAT GCAGAGATGA AGATTGTCGA TCCTGAAACC 
GGTCATTCTT TACCCAGAAA CCAATCCGGT GAAATTTGCA TAAGAGGCGA CCAGATTATG 
AAAGGTTATC TAAATGATGG AGAGGCTACA GAGAGAACCA TAGACAAAGA TGGTTGGTTG 
CACACAGGTG ACATCGGTTA CATCGACGAT GACGATGAGT TATTCATCGT TGACAGGCTC 
AAGGAATTGA TCAAATACAA AGGATTTCAA GTGGCTCCAG CTGAACTTGA AGCCCTTCTT 
CTCACTCATC CTAAGATCTC TGATGCTGCT GTTGTTCCAA TGAAGGATGA AGCCGCGGGA 
GAGGTACCTG TTGCATTTGT GGTCATATCA AATGGTTATA CCGACACAAC CGAGGATGAA 
ATTAAGCAGT TTATCTCCAA ACAGGTGGTG TTTTACAAAA GAATAAACCG AGTATTCTTC 
ATTGATGCAA TTCCCAAGTC ACCGTCAGGC AAAATCTTGC GCAAGGATCT AAGAGCAAAG 
ATAGCGGCAA GTGTTCCAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC547944 ( NM_001249489.1 ) cDNA ORF clone, Glycine max(Soybean) -> NP_001236418.1 Glycine max 4-coumarate:CoA ligase isoenzyme 2 (LOC547944), mRNA.

Price & Availability↑

CloneID	OGl54226
Clone ID Related Accession (Same CDS sequence)	NM_001249489.2 , NM_001249489.1
Accession Version	NM_001249489.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1644bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-10-28
Organism	Glycine max(Soybean)
Product	4-coumarate--CoA ligase 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF002259.1. ##Evidence-Data-START## Transcript exon combination :: AF002259.1, X69954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00264989, SAMN00769647 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGATG ATGGAAGCAG GAGGGAATTG ATATTCAGGT CGAAGCTTCC GGATATCTAC 
ATCCCCAAAC ATATGCCCCT CCACTCTTAC TGCTTCGAGA ATTTGAGAGA GTGTGGTTCA 
CGCCCCTGCC TGATCAATGC CCCCACGGGA GACGTCTACA GCTACCACGA GGTGGACAGC 
ACCGCCAGAA AGGTGGCGAG GGGGCTGAAG AAAGAGGGCG TGGAACAGGG CCAGGTCATC 
ATGATCCTCC TCCCCAATTG CCCCGAATTC GTCTTCTCGT TTCTTGGCGC ATCCCACCGC 
GGTGCCATGG CCACTGCCGC CAACCCTTTC TTCACCCCCG CCGAGATTGC AAAGCAAGCC 
CATGCCTCCA ATGCCAAGCT CCTCATCACC CAGGCCTCTT ACTACGACAA AGTCAAGGAC 
CTCCGCGACA TCAAGCTCGT ATTCGTCGAC TCTTGTCCCC CCCATACAGA GGAGAAGCAG 
CATCTCCATT TCTCACATCT GTGTGAGGAT AACGGTGACG CTGATGTTGA TGTTGATGTT 
GATATCAAGC CCGATGACGT GGTTGCATTG CCTTATTCTT CGGGAACAAC TGGTCTTCCC 
AAGGGAGTGA TGCTGAGCCA CAAGGGGCTG GTGACCAGCA TTGCTCAGCA GGTTGACGGT 
GATAATCCCA ACCTCTATTA CCACTGCCAC GACACCATCC TCTGCGTGCT TCCCCTCTTT 
CACATTTACT CTCTCAACTC CGTTCTTCTC TGTGGCTTGC GTGCCAAGGC TACCATTCTC 
CTCATGCCCA AGTTCGATAT TAACTCCTTG CTTGCTCTCA TTCACAAGCA CAAAGTCACT 
ATTGCCCCTG TCGTCCCTCC CATTGTTCTC GCCATTTCCA AATCACCCGA TCTCCACAAG 
TACGACCTGT CTTCCATCAG AGTCTTGAAG TCCGGGGGAG CCCCTCTGGG TAAGGAACTC 
GAAGACACTC TCAGAGCTAA ATTCCCCAAC GCCAAACTTG GCCAGGGATA CGGAATGACT 
GAGGCAGGGC CTGTGCTAAC AATGTCTTTA GCTTTTGCTA AAGAACCGAT AGACGTGAAA 
CCAGGTGCAT GTGGAACCGT TGTAAGAAAT GCAGAGATGA AGATTGTCGA TCCTGAAACC 
GGTCATTCTT TACCCAGAAA CCAATCCGGT GAAATTTGCA TAAGAGGCGA CCAGATTATG 
AAAGGTTATC TAAATGATGG AGAGGCTACA GAGAGAACCA TAGACAAAGA TGGTTGGTTG 
CACACAGGTG ACATCGGTTA CATCGACGAT GACGATGAGT TATTCATCGT TGACAGGCTC 
AAGGAATTGA TCAAATACAA AGGATTTCAA GTGGCTCCAG CTGAACTTGA AGCCCTTCTT 
CTCACTCATC CTAAGATCTC TGATGCTGCT GTTGTTCCAA TGAAGGATGA AGCCGCGGGA 
GAGGTACCTG TTGCATTTGT GGTCATATCA AATGGTTATA CCGACACAAC CGAGGATGAA 
ATTAAGCAGT TTATCTCCAA ACAGGTGGTG TTTTACAAAA GAATAAACCG AGTATTCTTC 
ATTGATGCAA TTCCCAAGTC ACCGTCAGGC AAAATCTTGC GCAAGGATCT AAGAGCAAAG 
ATAGCGGCAA GTGTTCCAAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001236418.1
CDS	67..1710
Translation	MADDGSRRELIFRSKLPDIYIPKHMPLHSYCFENLRECGSRPCLINAPTGDVYSYHEVDSTARKVARGLKKEGVEQGQVIMILLPNCPEFVFSFLGASHRGAMATAANPFFTPAEIAKQAHASNAKLLITQASYYDKVKDLRDIKLVFVDSCPPHTEEKQHLHFSHLCEDNGDADVDVDVDIKPDDVVALPYSSGTTGLPKGVMLSHKGLVTSIAQQVDGDNPNLYYHCHDTILCVLPLFHIYSLNSVLLCGLRAKATILLMPKFDINSLLALIHKHKVTIAPVVPPIVLAISKSPDLHKYDLSSIRVLKSGGAPLGKELEDTLRAKFPNAKLGQGYGMTEAGPVLTMSLAFAKEPIDVKPGACGTVVRNAEMKIVDPETGHSLPRNQSGEICIRGDQIMKGYLNDGEATERTIDKDGWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPKISDAAVVPMKDEAAGEVPVAFVVISNGYTDTTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKIAASVPK

Target ORF information:

RefSeq Version	NM_001249489.1
Organism	Glycine max(Soybean)
Definition	Glycine max 4-coumarate:CoA ligase isoenzyme 2 (LOC547944), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001249489.1

ORF Insert Sequence:

ATGGCTGATG ATGGAAGCAG GAGGGAATTG ATATTCAGGT CGAAGCTTCC GGATATCTAC 
ATCCCCAAAC ATATGCCCCT CCACTCTTAC TGCTTCGAGA ATTTGAGAGA GTGTGGTTCA 
CGCCCCTGCC TGATCAATGC CCCCACGGGA GACGTCTACA GCTACCACGA GGTGGACAGC 
ACCGCCAGAA AGGTGGCGAG GGGGCTGAAG AAAGAGGGCG TGGAACAGGG CCAGGTCATC 
ATGATCCTCC TCCCCAATTG CCCCGAATTC GTCTTCTCGT TTCTTGGCGC ATCCCACCGC 
GGTGCCATGG CCACTGCCGC CAACCCTTTC TTCACCCCCG CCGAGATTGC AAAGCAAGCC 
CATGCCTCCA ATGCCAAGCT CCTCATCACC CAGGCCTCTT ACTACGACAA AGTCAAGGAC 
CTCCGCGACA TCAAGCTCGT ATTCGTCGAC TCTTGTCCCC CCCATACAGA GGAGAAGCAG 
CATCTCCATT TCTCACATCT GTGTGAGGAT AACGGTGACG CTGATGTTGA TGTTGATGTT 
GATATCAAGC CCGATGACGT GGTTGCATTG CCTTATTCTT CGGGAACAAC TGGTCTTCCC 
AAGGGAGTGA TGCTGAGCCA CAAGGGGCTG GTGACCAGCA TTGCTCAGCA GGTTGACGGT 
GATAATCCCA ACCTCTATTA CCACTGCCAC GACACCATCC TCTGCGTGCT TCCCCTCTTT 
CACATTTACT CTCTCAACTC CGTTCTTCTC TGTGGCTTGC GTGCCAAGGC TACCATTCTC 
CTCATGCCCA AGTTCGATAT TAACTCCTTG CTTGCTCTCA TTCACAAGCA CAAAGTCACT 
ATTGCCCCTG TCGTCCCTCC CATTGTTCTC GCCATTTCCA AATCACCCGA TCTCCACAAG 
TACGACCTGT CTTCCATCAG AGTCTTGAAG TCCGGGGGAG CCCCTCTGGG TAAGGAACTC 
GAAGACACTC TCAGAGCTAA ATTCCCCAAC GCCAAACTTG GCCAGGGATA CGGAATGACT 
GAGGCAGGGC CTGTGCTAAC AATGTCTTTA GCTTTTGCTA AAGAACCGAT AGACGTGAAA 
CCAGGTGCAT GTGGAACCGT TGTAAGAAAT GCAGAGATGA AGATTGTCGA TCCTGAAACC 
GGTCATTCTT TACCCAGAAA CCAATCCGGT GAAATTTGCA TAAGAGGCGA CCAGATTATG 
AAAGGTTATC TAAATGATGG AGAGGCTACA GAGAGAACCA TAGACAAAGA TGGTTGGTTG 
CACACAGGTG ACATCGGTTA CATCGACGAT GACGATGAGT TATTCATCGT TGACAGGCTC 
AAGGAATTGA TCAAATACAA AGGATTTCAA GTGGCTCCAG CTGAACTTGA AGCCCTTCTT 
CTCACTCATC CTAAGATCTC TGATGCTGCT GTTGTTCCAA TGAAGGATGA AGCCGCGGGA 
GAGGTACCTG TTGCATTTGT GGTCATATCA AATGGTTATA CCGACACAAC CGAGGATGAA 
ATTAAGCAGT TTATCTCCAA ACAGGTGGTG TTTTACAAAA GAATAAACCG AGTATTCTTC 
ATTGATGCAA TTCCCAAGTC ACCGTCAGGC AAAATCTTGC GCAAGGATCT AAGAGCAAAG 
ATAGCGGCAA GTGTTCCAAA ATGA