HECTD4 cDNA ORF clone, Gallus gallus(chicken)

The following HECTD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa144809 XM_025155665.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42923 XM_015275539.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42924 XM_015275540.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42925 XM_015275541.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42926 XM_015275542.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42927 XM_015275543.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OGa42928 XM_015275544.1
Latest version!
Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa144809
    Clone ID Related Accession (Same CDS sequence) XM_025155665.1
    Accession Version XM_025155665.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product LOW QUALITY PROTEIN: probable E3 ubiquitin-protein ligase HECTD4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006102.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

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    181
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    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
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    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    7021
    7081
    7141
    7201
    7261
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    7381
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    7501
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    7741
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    8401
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    8701
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    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
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    10921
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    11101
    11161
    11221
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    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACCGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTAGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCATTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TTGGTGATCT ATGCTGGTCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTAGTAAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGGA GTGGTCGTGA GCACAACACT CCAGACAAAG CCATGTGGGG CTTTGCTTGC
    ACTGTTCGAG CACAGGAGTC TTCAGAGGAT GTGTCAGGAG GCCTGCCATT CTTAGTTGAC
    CTGGCACTTG GCCTTTCTGT GTTAGCTTGT TCTATGCTTC GGATTTTGTA CAATGGACCA
    GAAATTACCA AAGATGAAGA AACCTGTCAA GAGCTTCTAA GATCTAAACT ATTACAAAGG
    TGCCAATGGC AGGTGGAAGC TAATGGTGTA ATATCTCCAG CCCTTACACC GAGTCCTTCT
    CCACTGCCTC TTACTATAGA TGAGGACAGA GAATTTACTT ATCCTTCTGA TGTGCTGGTG
    CCTCCCATTG GACACTATTT TGATCTGCCT AGGATTAGGC TGCCTCCAGG AATCATGATA
    AAACTCAGGG AAATTTCTGG ACGTGCTCGG CCTCAATTTA GACCTAGCAT AAAGGAAGTG
    ATTCAGCCCG AGGTAATGGA GGAGATGGTA GTTTCATGTG TGATTAAACA TTTGAATTTG
    GTTGATGCAC TCCAGTCCCT AATAAACTTC CAGTATCAGG AAGAACATGC TGAAGAATAT
    GATTTACTTT GTAAAATTAT GGGAGAGACT TTTAAGAAGC TAAATGCCAT GGAGAGACAA
    TTACAGAGTG TGGCTGAGCT GGAACAGAAA TGGCAGAGTG AGGTTGATGA CGCCATGCAT
    GGGAAGCTGG AGAATAATGT TCCCTTCTTC TATGATTACC ACTTCAATGA GAGCAAGATG
    AAAGAACTGG AACTTCTGTG TTCTATGAAG GAAGTTTCTT TTGATGGAAG TGATCTTGAG
    AATGTGGTCC TGGCATTAAG GGAGAAGTTC TTTCAAGAAG TGAATTCACT GATACAGAAG
    ACTTATCATC CCCTAGCAAA AACAAAAGCA TTAGTTAAAA GCTTGATGAA CAGGGCAGAG
    CTGTTGCTGC ATGTCACTAT TGCAGCGCAG TCTGGTATCA CAAGAAGTAT ATCTGGAACC
    CCTGCAGAGA CTCCAGCCTG TAAGTCTGCA TCTGAAACAA AAGTGATATC TCATGCAGTG
    CGCCAGCCCG TCTTCCTTCG CAGTATGTCA GCACCATCTG ACTTAGAGAT GATTGGTAAC
    GAAGATATTG AGTTTGCTCG ATCAAGTCAA AGGCGACGCC ATGTTACCAG CCACAGAAGT
    AGCTCTTTCA CTCTTCTGCA ATCACTGACC ATTGAGGACA GCAGAGATAA ACCTACATAC
    AGTGTCTTAC TGGGGCAGCT CTTTGCTTTC ATTGGAACTA ATCCTGACCA AGCGGTATCC
    AGCAGCAGTT TTCTGCTCGC TGCTCAGACA AGGTGGCGGC GTGGGAACAC AAGGAAGCAA
    GCCTTAGTGC ATATGAGAGA GCTGCTGACT GCGGCTGTTC GGGTTGGTGG GGTGACACAC
    CTCGTGGGCC CTGTGACCAT GGTACTTCAG GGAGGACCAA GAATTGAAGA GCTCACGTGT
    GGTGGGATGG TGGAACAAGT CCAGGAAGCT TTTGGGGAGA CTATGACATC CGTGGTCTCG
    CTGTGTGCTC GCTACCCCAT TGCTTGTGCA AACAGCATTG GCCTCTTATG CACTATACCT
    TACACAAGGA GTGAAGAGAA GTGTTTGGTA CGTAGTGGCC TGGTACAGCT TATGGACAGG
    CTGTGCAGCT TAAGCAGCCA GGCAGAATCC AGTTCAAATG AAAAACAGAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCTTG GGCTGCCTTT CAGGTGCTGG CCAATCGCTG CGTTGAATGG
    GAGAAAGAAG AAGGTGGTTC AACAGAAGCT GTTCACTCTG GTCTGGCTCG TCAGGTCTCC
    AGCCTTCTTA CCAATCACCT TGCACGAGCT ACAGAGTGTT GTGGTAATCA AGCTGCAGGA
    AATGATGCAC TTCAAGACGT CCTCAGTCTT CTTAATGACC TGTCGAGGAG TCACATAGGT
    AAAGCAATCC TGAGCCAGCC AGCCTGTGTG TCCAAACTTC TGTCACTTCT CTTGGATCAA
    CGTCCCTCTC CAAAGTTGGT TCTTATAATC CTTCAGTTGT GTCGTGCTGC TCTACCTCTC
    ATGAGTGTTG AAGACTGTGG AAATGTAGAA CTGCCTCCCT GGAGTTACTC TGTGCCCTCC
    CTGAACAGTG AACAAGATGA TCCCAGCGAC CCAGCCTCCA AGATTGCATC TCTGCTCTTA
    GCAAAGCTGG CAGATTATGT AGTACCAGGA TGCCAGACAG TTCTTTCTCC AACTGCTTCT
    GAACCAGATA CTGCTTTAGC AAAAAGCAGC CCTAAGAATT CTATAAAAGG CGATAAAGAC
    CCTGGAGAAG AAAGTGAGGC AGTGGATGGC AAACTTTCTA TTTTCATCCA TAAACGGGAA
    GACCAGTCAT CCCACGAAGT GCTTCAGCCA TTGCTGAGTA GCTCCGAAGG TCGCCCATTC
    CGACTAGGAA CTGGAGCCAA CATGGAGAAG GTGGTGAAAA TGGATCGTGA CATGACTAAA
    AGTGGCTGCT GTGAAGTTAT CACAGAAGAA GCTGCTGCAG CTCTTCGCAA GGCAACTAAG
    TGGGCTCAGT CTGGTCTGAT AGTTAGTGTT GGGCCACCCA TAGAAACTGT CAATCCAGAA
    GCAACCAGTG GGATGTCCTC TGGGGACAAA AAGAAGACTG CACAGACATC TATTTGCAGA
    GAGAGAAATT CTGAGCTTGC CAGGACTGAT CCTGTAAGAC CATTCATCAG TGGTCATGTT
    GCAAATAGCA TGGCAGCAGA AGTGATCGCT TTGCTACATA GCTTGTTGAT GGCACCAGAA
    TCAAATGCGG CTCAAATATG GACAACAACT GCTGAAAAAG TTCTGTCTCG GGCTCTGATG
    TACATTCCAC AACTCGGGAA ATACGCAGAG AGTATTTTGG AGAATGGAAG CAGCAGTGGA
    AGGAAGCTTG CTAAACTTCA AAGAATTGCT CGACAGGCAG TAGCTGCTCT TTGTGCTCTA
    GGTGGCTTTA AGGAGACAAT TAAAATCGGT TCTGAAGTTC AGGTTTTAGG TAAAGGAATA
    GCAGGAAGCA TTGGAGTTGT GGCATCTATT AATGAACAAG AAGGTATAGC AACGGTCAAA
    TTTCCGCCTA CCACTATAGA CAGTAGAAAG ACCTCACAAG CATCAGACAC TTTAACTATT
    CCGTTATCTA GGCTCTGTGT TCCAAGATCT GAGGCATTGC CTCTTCATAA ACTGTCCATT
    ACTGAGAAGG TAGTACAGGC AGTCCAGTCC ATGTTGCTTC CTCAGGAGGG AAGTCTATCT
    ATTCACACCT CACTTCCTGC AACAGGAGAT GGCTCAACTC CAGTAATGGC AGTGGTCCGG
    CTCCTTGCTG AAATAAGAAC CAGAGCATGC CTGGTGATGG CTCAGCTGTT AGAAGACAGT
    TCATTCTGTG AAGAATTCAT TCAACAGTGC CCAGCTGCTG TAGAGGTTCT TAATTTGGTA
    GCCCAGGAGT GCAGCCCTGG TAGAGAGGCT CACTGTGTAG AAATGCAGTG TGAAAGGTTA
    AGGATGTTGT ATCGTGACTG TGCTCGACCC CCACCTCCTC CACTGCAGGC AGACAGAAGG
    CAGCCCAAAG AAATCACTTG GAGCCCATCA AGAGTGTTTC CCCCAGTACG AGCATGCATG
    TTTTCATCAC ATCTTGCTGC TGTGACCTTC CTGGCTGATC CAAGTGCAGG TGGGGGACTT
    CCTCGGGGAA CGTTCATTTA TGCCACTTCT CCAGTTCCCG TGCAGGCACC ATCATTCTAC
    TGGGAAATTG AAATAGTTTC CTATGGAGAC ACAGATGATG ACACTGGTCC AATAGTTTCA
    TTTGGATTCG CTACAGAGGC AGAAAAACGG GATGGGGCTT GGACAAACCC AGTGGGCACC
    TGTCTTTTTC ATAACAATGG GCGAGCAGTG CATTACAATG GCTCCAGCTT GTTACAGTGG
    AAGAGTGTTC GATTGGATGT GACTCTTTCT CCTGGTGATG TGGCTGGAAT TGGATGGGAA
    AGAGCAGAAG GAACTCCACC CCCACCAGGG CAGCCTGCTA AAGGCAGAGT TTATTTTACT
    TATTGCGGGC AGCGACTTGG GCCATACCTA GAGGATGTCT CTGGTGGAAT GTGGCCAATT
    GTTCACATAC AGAAAAAGAA CACAAAAGTC CGTGCTAATT TTGGATCTCG CCAGTTTGCC
    TATGCCGAAG GCCAAGCTCA CAGGAATGCT GCTGATCTGT GTATTGACCT AGCTGAAGAG
    ATAAGTGCCA ACTTTGAGGC ATTGCCATTT GCTATGGCTT CTGATAGCGA TAACGACGCT
    GGCACCAGCA TAGCTTCTGA TCCTGGGACT CACGGACCTC CCTGTAGAAT TGCTGCTGTT
    GCTACGGCTC AACAACAATA TGACAGTGAC ACANTCTGTC ACTATAAAAT GGAACTAAGC
    TATGAGAACT TCATCACATC AGGGCCTGAT CCTCATCCCC CTCCTATTGC AGATGATGAA
    AGTGATGATG ACGACGATGA TGATATTCCC AGGGAGGACC ACTATGCCCT GCTGGTTAAA
    GCCTGGGAAA CTAAAGTTTT CCCTACAATT AGAAGAAGAT TCCGTAATGA GGCTGAGAGG
    AAATCTGGGC TGGATCAAAT TAAAGGAGCC TTACAGTTAG GAATGGTTGA TATAGCACGA
    CAAACTGTAG AATTCTTGTA TGAGGAAAAC GGTGGCATCC CAAGAGACCT CTACCTTCCT
    ACCATTGAGG ATATCAAAGA TGAAGCAAAC AAGTTTACAA TCGATAAAGT ACGAAAAGGT
    CTCACTGTTG TGACTCGCTC TCCTGACAGT AACAACGTTA CCAGCAGTGC TGTTGGAACA
    GCTTTACCCA AGTTTGCTAT TCGGGGAATG CTGAAGACAT TTGGTCTTCA TGGTGTTGTC
    CTGGATGTTG ATTCAGTGAA TGAATTGGTA CAAGTAGAGA CCTATCTCAG AAGTGAGGGA
    GTTCTGGTAA GATATTGGTA TCCTATTGAC ATGTTAGAAA GGCCACCTGC TGGTTATAGG
    AGGACTGCAA CAAATGGATT GGTTACCCTG GACAATACCA ATATCCAAAT CCACAGAGAG
    CTTTTGAGAA GTGAAGCTGC ACTGGCCAAG CTGTACTGCC GCATGGCTCT CCTCAATATC
    TTTGCCCCCA AATCTCCACA TATGTTCACC CGTCTGTTTC ATATCCCCGC TATCCGTGAT
    ATCACTCTGG AGCACCTGCA GTTGCTATCC AACCAACTTC TCGCACCACC TCTTCCTGAT
    GGAACAATCA GTTCAAGTTC CATTCTTTTG GCTCAGTCAC TGCAACACTG CATCCATTCC
    CAGAACTGTG CTGCCACAGA CCTCTTCTAC CAGGGCAATT CACAAGCCAT CAGAGAGTGG
    CTTACTGTTG CCATCACTCG GACACTGCAT CAAGGAGAGG AGAGCCTGTT AGACCTCACT
    AAGCAGATCT GCTCTTTTTT GCAGACTGCA CCAGAACAGT TTCCTGCTGA AGAATTCCCA
    ATTTCAGAAT CCAAAGTCAA CATGGATGTT AATTTTCCTG GGGCTGCGTT TGTAATTGTG
    TCCTGTAAAG AAAGCCAGTC TGGATTCCGC AAAGATTCTT CCCTGTATAA AGCTCCTTGG
    GCTCGGGTGC TTGTGTACGG GCTGGGGCAC AAAGTTAAGA GGAATGGCCA GTTAAACCTG
    ATTGAGGCAG TGTGTTACCC TCGAGACGCC TCTCCGACCA ACACAGGCCT AACACCTCCC
    CCAACAACCA ATCAGTACCC TTCAGTGATC ACTCCTACAG ACAAAGTGCA CATCAAACTA
    GGAGTGTCTC CTCCTCCTGG AGCTGTGCTG GTCTTGCATT CTCTTCCCCT TGAGTTCCCC
    CTGGCAATGG CATTTACAGA ACAGCTGTTA ACTTGGAAAC TGGAAGATGT GGATGGGAAA
    TCAGAAGATG AACTGGATAC TATTCCTCCT TCAGTTCTTT TGCAAGTAGT GGAATTTTTA
    GGGAACTTTC TGTGGACAAC TGACATGGCA GCATCTGTAA AGGAATTGAT ATTTCATCTC
    TTGGCTGAAT TGCTTCGCAA AATTCACAAC CTGGAGCAGA AGAAAAGTCC TGCAGGATTA
    TCATCTTCTA TTGCCCTTCA GCTGAATCCC TGTCTAGCTA TGCTTATGGC CCTGCAGTCA
    GAACTCCATA AGCTGTACGA TGAGGAAACA CAAAACTGGG TGTCTGGAAA TGCATGCGGT
    GGTTCTGGTG TTGGAGCTGC TGACCAAGGA AGATTTTCAA CATACTTCCA TGCTCTGATG
    GAAGTTTGCC TTGCTGTAGC AGAAGTAACT CTACCTATCA ATATGAGCGT AACAGCTAAT
    GTGATATCTT CAACGAGTGC ACCAAATCTT AGTGACTCTT CCTCCTCATC TTCATCTTCT
    CCAGGACAGA CACCACAGAG TCCAAGCTTA TTGTCAAAGA GGAAAAAAGT CAAAATGAAG
    AGAGAAAAGA CATCGTCTTC AGGCAAACGA TCTTCATCCA GAGCAGCTGA AACGGATGCT
    GCAATCCTCA GTATAGGAGG AAGCAAGCCT GAGGATATGC TATGGTTTCA TAGAGCACTG
    ACTTTGCTTA TCATTCTCAG ACACCTAACG AAGAAGGATC CACAAGGGTT GGGTGTGACA
    AACGATGCTG TAGCAGATGC ATGTCAGGCA CTGGTAGGAC CCACAGCTCA TAGCCGTTTG
    CTTGTCATTT CTGGAATACC AACTCACTTG GAGGAGAGTA CTGTCAGAAA TGCAATCAGG
    AAGGCGTGTA ATGCGCATGG TGGAGTCTTC AAGGATGAAA TCTACATCCC TCTGCAGGAA
    GAGGATCCCA AAAAAATCAA GGACAAAGCA GAAGGAGGAG AATGCAGAAC TGAGCTGGAA
    AAGCCAACTG TTTCAAGTAG TGCGGACAGT CTGGATATCA GCAGCTCTAG CAGTGTCACA
    CCTGCTATGA GCGTTAGTGC GTCAGCATCA ACGAGCCAGG CTTCCCTGTG CAGCTCACAG
    GGTATCTCCC GGACTGTTAG TGACATCTCT GTTGATCAGT ACCAGGCTGG GCTGGAACTG
    GCCATCCCAC CAGGACTGCT GGAGCCACAC GTTTCCAGCC AGGAGAGCTT GGATCTGTCG
    CACTGCAGTA CTGGGAGCCT TGGCAGCCTT GGCAGCCTCG GGGAGCCGCT TGACAATGTA
    GAGACGGCGT CAGTGTCTGA TGTCGGGTCG ATGTACACAG TCACATCTCT GGACAACCAG
    CAACTTCTGT CACGGCCAAT CAAAGGTTTT GCTGTGGTGG AGATACGATC AAGAGCTAAA
    ATAGAGAAAA TCCGAGCCAG CCTATTTAAC AGTAGTGATC TCATTGGTTT GTCAAGTCTG
    GATGGTGAAG ACGAACTGAT GGAAATGTCA ACTGAAGAGA TTTTAACTGT TTCTACTGTA
    AATCAGAGTT TATTTGACAC ACAAGGGAGC CCGGCGCTAG AAGACTACTT CAATGATAAG
    TCAATAAAAG GTGAGAAATT GGTTCCTGGT GCTCGGGAGG TCCTAACAGA AATATTTAAA
    AGCTGTGTCC ATTCTGAGCA AATGCTGAGC CTGACTCCAA CAAAACCAAT CAAGGTTGCT
    GATATCTATC TTAGTAAAGA GCAGATAAAT TCTCAGACTC CTGGAAACCT TCTTCATGTC
    TTCTTCACAA ATGTCCGTCC TCCAAAGAAG GTGCTGGAGG ACCAGCTTAC TCAGATTCTA
    AGGAAATACG GTGTGCCCAA GCCGAAATTT GACAAGAGCA AATACAACAA GGCAGGCAAA
    GAACAGCATC CGGTGAAGGT TGTGAGCACC AAGCGCCCAG TTACAAAACC ACCCACAAAA
    GAAAAGTCTG TACTCAACAG CGTCAGCCGA ACTGCTTTGA GTGAGAAGAA ACCTACTGTA
    AAGCCAAAGT CTCCTGAGAA GAGCAAACCT GAAGAGAAGG ACCCTGAAAA GTCTCCCACA
    AAGAAACAAG AAGTTCCTGA GGAGAAGTAC CTGACACTGG ATGGATTTCA CAGATTTGTC
    ATTGACCGAT CTAAGCAGGA TATCCGTAGT GTGTGGAGAG CTATTCTGTC CTGTGGATAT
    GATCTGCATT TTGAAAGATG TGCCTGCATT GATGCAAGAC ATGCACAGAA AGCATCTCGG
    AAGTGGACTC TGGAAATGGA CGTGGCTCTC GTTCAGTACA TTAACAGGCT TTGTCGTCAC
    CTCGCAATTA CACCAGCACG ACTCCATCCA CATGAAGTAT ATTTGGATCC AGCTGATGCA
    GCAGATCCTA GAATCTCCTG TCTGTTGAAT GTTCCCGTTG AAAGTCTCCG CCTGCGGTTT
    GCACTGCTTC AGTCTCTCAA TACGACGTTA GAGACGTTTT TCTTACCGCT GGTAGAGCTT
    CGGCAGACTG AGATGTACAG TAACAGCATT GCTGCACTGC TGCAGGAGGC CAAAGGTTTA
    ATCTTCTATG ACACAAAAAT AACCGTAATG AACAGAGTGC TGAATGCCAC TGTCCAAAGA
    ACTGCTGATC ACGCAGCCCC AGAAATCACC TTGGATCCCT TGGAAATAGT TGGAGGGGAG
    ATCCGTTCTT CAGAAAATTC CTACTTCTGC CAGGCAGCAA GGCAGCTGGC CTGTGTGCCA
    TCCTCACAGC TCTGCGTCAA ACTCGCCAGT GGTGGGGACC CTACGTACGC GTTCAACATC
    CGGTTCACAG GGGAGGAGGT CCATGGAACA AGTGGATCTT TCCGTCACTT CCTCTGGCAA
    GTTTGTAAGG AGCTGCAGAG CTCCTCACTC TCCCTTCTCC TGTTGTGTCC AAGCTCTGCG
    GTCAACAAGA ACAAGGGGAA GTACATTTTG ACACCTAGCC CCATAACCTA TGCAGAGGAG
    CAGCTGTTCC ATTTCTTTGG GCAGCTTCTG GGCATTGCAA TTCGAGCAGA TGTTCCCCTG
    CCACTCGACC TCCTGCCCTC ATTCTGGAAG ACTCTGGTAG GAGAGCCGCT GGATCCTGAT
    GTTGATCTCC AGGAAGCTGA TATCCTCACC TACAACTACA TTAAAAAGTT TGAAAATATA
    AATGATGAAA CTGAACTGGA AGCTCTGTGT GCAGAAATTG CTTCTCAGCA TTTAGCAACA
    GAGAGCCCTG ACTGTCCTAA TAAGCCATGC TGCAAATTCA CCTACCTGAC CATGACAGGG
    GAAGAGGTGG AACTCTGTCC CAGGGGCAGG CACGTCCCAG TGGGATGGGA GAACAAGGAC
    GTGTATGCAG CTGCAATCAG GAGCTTGAGA ATGCGTGAGC TGCAGACTCC AGAGTGCATG
    ACTGCAGTGC GTGCTGGGCT TGGGTCCATC ATTCCCCTGC AGCTTCTGAC CACGCTGACC
    CCTCTTGAGA TGGAGCTGAG GACATGTGGG CTTCCATACA TCAACCTGGA GTTTCTCAAG
    GCGCACACGA TGTACCAGGT GGGACTGATG GAAACTGATC AGCACATTGA GTTTTTTTGG
    AGCGCGCTGG AGATGTTCAC TCAGGAGGAG CTCTGCAAGT TCATAAAGTT TGCCTGTAAC
    CAGGAGCGGA TCCCCTTCAC GTGTCCTTGC AAGGACGGCG GTCCCGATAC CGCCCACGTC
    CCGCCTTACC CCATGAAAAT AGCCCCCCCT GATGGAGCTG CAGGTTCTCC GGACTCTCGG
    TACATCCGTG TGGAAACGTG CATGTTCATG ATCAAACTTC CTCAGTACTC ATCACTGGAC
    ATCATGCTGG AGAAGCTCCG ATACGCCATC CACTACCGGG AGGATCCTCT CAGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025011433.1
    CDS264..13523
    Translation

    Target ORF information:

    RefSeq Version XM_025155665.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025155665.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACCGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTAGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCATTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TTGGTGATCT ATGCTGGTCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTAGTAAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGGA GTGGTCGTGA GCACAACACT CCAGACAAAG CCATGTGGGG CTTTGCTTGC
    ACTGTTCGAG CACAGGAGTC TTCAGAGGAT GTGTCAGGAG GCCTGCCATT CTTAGTTGAC
    CTGGCACTTG GCCTTTCTGT GTTAGCTTGT TCTATGCTTC GGATTTTGTA CAATGGACCA
    GAAATTACCA AAGATGAAGA AACCTGTCAA GAGCTTCTAA GATCTAAACT ATTACAAAGG
    TGCCAATGGC AGGTGGAAGC TAATGGTGTA ATATCTCCAG CCCTTACACC GAGTCCTTCT
    CCACTGCCTC TTACTATAGA TGAGGACAGA GAATTTACTT ATCCTTCTGA TGTGCTGGTG
    CCTCCCATTG GACACTATTT TGATCTGCCT AGGATTAGGC TGCCTCCAGG AATCATGATA
    AAACTCAGGG AAATTTCTGG ACGTGCTCGG CCTCAATTTA GACCTAGCAT AAAGGAAGTG
    ATTCAGCCCG AGGTAATGGA GGAGATGGTA GTTTCATGTG TGATTAAACA TTTGAATTTG
    GTTGATGCAC TCCAGTCCCT AATAAACTTC CAGTATCAGG AAGAACATGC TGAAGAATAT
    GATTTACTTT GTAAAATTAT GGGAGAGACT TTTAAGAAGC TAAATGCCAT GGAGAGACAA
    TTACAGAGTG TGGCTGAGCT GGAACAGAAA TGGCAGAGTG AGGTTGATGA CGCCATGCAT
    GGGAAGCTGG AGAATAATGT TCCCTTCTTC TATGATTACC ACTTCAATGA GAGCAAGATG
    AAAGAACTGG AACTTCTGTG TTCTATGAAG GAAGTTTCTT TTGATGGAAG TGATCTTGAG
    AATGTGGTCC TGGCATTAAG GGAGAAGTTC TTTCAAGAAG TGAATTCACT GATACAGAAG
    ACTTATCATC CCCTAGCAAA AACAAAAGCA TTAGTTAAAA GCTTGATGAA CAGGGCAGAG
    CTGTTGCTGC ATGTCACTAT TGCAGCGCAG TCTGGTATCA CAAGAAGTAT ATCTGGAACC
    CCTGCAGAGA CTCCAGCCTG TAAGTCTGCA TCTGAAACAA AAGTGATATC TCATGCAGTG
    CGCCAGCCCG TCTTCCTTCG CAGTATGTCA GCACCATCTG ACTTAGAGAT GATTGGTAAC
    GAAGATATTG AGTTTGCTCG ATCAAGTCAA AGGCGACGCC ATGTTACCAG CCACAGAAGT
    AGCTCTTTCA CTCTTCTGCA ATCACTGACC ATTGAGGACA GCAGAGATAA ACCTACATAC
    AGTGTCTTAC TGGGGCAGCT CTTTGCTTTC ATTGGAACTA ATCCTGACCA AGCGGTATCC
    AGCAGCAGTT TTCTGCTCGC TGCTCAGACA AGGTGGCGGC GTGGGAACAC AAGGAAGCAA
    GCCTTAGTGC ATATGAGAGA GCTGCTGACT GCGGCTGTTC GGGTTGGTGG GGTGACACAC
    CTCGTGGGCC CTGTGACCAT GGTACTTCAG GGAGGACCAA GAATTGAAGA GCTCACGTGT
    GGTGGGATGG TGGAACAAGT CCAGGAAGCT TTTGGGGAGA CTATGACATC CGTGGTCTCG
    CTGTGTGCTC GCTACCCCAT TGCTTGTGCA AACAGCATTG GCCTCTTATG CACTATACCT
    TACACAAGGA GTGAAGAGAA GTGTTTGGTA CGTAGTGGCC TGGTACAGCT TATGGACAGG
    CTGTGCAGCT TAAGCAGCCA GGCAGAATCC AGTTCAAATG AAAAACAGAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCTTG GGCTGCCTTT CAGGTGCTGG CCAATCGCTG CGTTGAATGG
    GAGAAAGAAG AAGGTGGTTC AACAGAAGCT GTTCACTCTG GTCTGGCTCG TCAGGTCTCC
    AGCCTTCTTA CCAATCACCT TGCACGAGCT ACAGAGTGTT GTGGTAATCA AGCTGCAGGA
    AATGATGCAC TTCAAGACGT CCTCAGTCTT CTTAATGACC TGTCGAGGAG TCACATAGGT
    AAAGCAATCC TGAGCCAGCC AGCCTGTGTG TCCAAACTTC TGTCACTTCT CTTGGATCAA
    CGTCCCTCTC CAAAGTTGGT TCTTATAATC CTTCAGTTGT GTCGTGCTGC TCTACCTCTC
    ATGAGTGTTG AAGACTGTGG AAATGTAGAA CTGCCTCCCT GGAGTTACTC TGTGCCCTCC
    CTGAACAGTG AACAAGATGA TCCCAGCGAC CCAGCCTCCA AGATTGCATC TCTGCTCTTA
    GCAAAGCTGG CAGATTATGT AGTACCAGGA TGCCAGACAG TTCTTTCTCC AACTGCTTCT
    GAACCAGATA CTGCTTTAGC AAAAAGCAGC CCTAAGAATT CTATAAAAGG CGATAAAGAC
    CCTGGAGAAG AAAGTGAGGC AGTGGATGGC AAACTTTCTA TTTTCATCCA TAAACGGGAA
    GACCAGTCAT CCCACGAAGT GCTTCAGCCA TTGCTGAGTA GCTCCGAAGG TCGCCCATTC
    CGACTAGGAA CTGGAGCCAA CATGGAGAAG GTGGTGAAAA TGGATCGTGA CATGACTAAA
    AGTGGCTGCT GTGAAGTTAT CACAGAAGAA GCTGCTGCAG CTCTTCGCAA GGCAACTAAG
    TGGGCTCAGT CTGGTCTGAT AGTTAGTGTT GGGCCACCCA TAGAAACTGT CAATCCAGAA
    GCAACCAGTG GGATGTCCTC TGGGGACAAA AAGAAGACTG CACAGACATC TATTTGCAGA
    GAGAGAAATT CTGAGCTTGC CAGGACTGAT CCTGTAAGAC CATTCATCAG TGGTCATGTT
    GCAAATAGCA TGGCAGCAGA AGTGATCGCT TTGCTACATA GCTTGTTGAT GGCACCAGAA
    TCAAATGCGG CTCAAATATG GACAACAACT GCTGAAAAAG TTCTGTCTCG GGCTCTGATG
    TACATTCCAC AACTCGGGAA ATACGCAGAG AGTATTTTGG AGAATGGAAG CAGCAGTGGA
    AGGAAGCTTG CTAAACTTCA AAGAATTGCT CGACAGGCAG TAGCTGCTCT TTGTGCTCTA
    GGTGGCTTTA AGGAGACAAT TAAAATCGGT TCTGAAGTTC AGGTTTTAGG TAAAGGAATA
    GCAGGAAGCA TTGGAGTTGT GGCATCTATT AATGAACAAG AAGGTATAGC AACGGTCAAA
    TTTCCGCCTA CCACTATAGA CAGTAGAAAG ACCTCACAAG CATCAGACAC TTTAACTATT
    CCGTTATCTA GGCTCTGTGT TCCAAGATCT GAGGCATTGC CTCTTCATAA ACTGTCCATT
    ACTGAGAAGG TAGTACAGGC AGTCCAGTCC ATGTTGCTTC CTCAGGAGGG AAGTCTATCT
    ATTCACACCT CACTTCCTGC AACAGGAGAT GGCTCAACTC CAGTAATGGC AGTGGTCCGG
    CTCCTTGCTG AAATAAGAAC CAGAGCATGC CTGGTGATGG CTCAGCTGTT AGAAGACAGT
    TCATTCTGTG AAGAATTCAT TCAACAGTGC CCAGCTGCTG TAGAGGTTCT TAATTTGGTA
    GCCCAGGAGT GCAGCCCTGG TAGAGAGGCT CACTGTGTAG AAATGCAGTG TGAAAGGTTA
    AGGATGTTGT ATCGTGACTG TGCTCGACCC CCACCTCCTC CACTGCAGGC AGACAGAAGG
    CAGCCCAAAG AAATCACTTG GAGCCCATCA AGAGTGTTTC CCCCAGTACG AGCATGCATG
    TTTTCATCAC ATCTTGCTGC TGTGACCTTC CTGGCTGATC CAAGTGCAGG TGGGGGACTT
    CCTCGGGGAA CGTTCATTTA TGCCACTTCT CCAGTTCCCG TGCAGGCACC ATCATTCTAC
    TGGGAAATTG AAATAGTTTC CTATGGAGAC ACAGATGATG ACACTGGTCC AATAGTTTCA
    TTTGGATTCG CTACAGAGGC AGAAAAACGG GATGGGGCTT GGACAAACCC AGTGGGCACC
    TGTCTTTTTC ATAACAATGG GCGAGCAGTG CATTACAATG GCTCCAGCTT GTTACAGTGG
    AAGAGTGTTC GATTGGATGT GACTCTTTCT CCTGGTGATG TGGCTGGAAT TGGATGGGAA
    AGAGCAGAAG GAACTCCACC CCCACCAGGG CAGCCTGCTA AAGGCAGAGT TTATTTTACT
    TATTGCGGGC AGCGACTTGG GCCATACCTA GAGGATGTCT CTGGTGGAAT GTGGCCAATT
    GTTCACATAC AGAAAAAGAA CACAAAAGTC CGTGCTAATT TTGGATCTCG CCAGTTTGCC
    TATGCCGAAG GCCAAGCTCA CAGGAATGCT GCTGATCTGT GTATTGACCT AGCTGAAGAG
    ATAAGTGCCA ACTTTGAGGC ATTGCCATTT GCTATGGCTT CTGATAGCGA TAACGACGCT
    GGCACCAGCA TAGCTTCTGA TCCTGGGACT CACGGACCTC CCTGTAGAAT TGCTGCTGTT
    GCTACGGCTC AACAACAATA TGACAGTGAC ACANTCTGTC ACTATAAAAT GGAACTAAGC
    TATGAGAACT TCATCACATC AGGGCCTGAT CCTCATCCCC CTCCTATTGC AGATGATGAA
    AGTGATGATG ACGACGATGA TGATATTCCC AGGGAGGACC ACTATGCCCT GCTGGTTAAA
    GCCTGGGAAA CTAAAGTTTT CCCTACAATT AGAAGAAGAT TCCGTAATGA GGCTGAGAGG
    AAATCTGGGC TGGATCAAAT TAAAGGAGCC TTACAGTTAG GAATGGTTGA TATAGCACGA
    CAAACTGTAG AATTCTTGTA TGAGGAAAAC GGTGGCATCC CAAGAGACCT CTACCTTCCT
    ACCATTGAGG ATATCAAAGA TGAAGCAAAC AAGTTTACAA TCGATAAAGT ACGAAAAGGT
    CTCACTGTTG TGACTCGCTC TCCTGACAGT AACAACGTTA CCAGCAGTGC TGTTGGAACA
    GCTTTACCCA AGTTTGCTAT TCGGGGAATG CTGAAGACAT TTGGTCTTCA TGGTGTTGTC
    CTGGATGTTG ATTCAGTGAA TGAATTGGTA CAAGTAGAGA CCTATCTCAG AAGTGAGGGA
    GTTCTGGTAA GATATTGGTA TCCTATTGAC ATGTTAGAAA GGCCACCTGC TGGTTATAGG
    AGGACTGCAA CAAATGGATT GGTTACCCTG GACAATACCA ATATCCAAAT CCACAGAGAG
    CTTTTGAGAA GTGAAGCTGC ACTGGCCAAG CTGTACTGCC GCATGGCTCT CCTCAATATC
    TTTGCCCCCA AATCTCCACA TATGTTCACC CGTCTGTTTC ATATCCCCGC TATCCGTGAT
    ATCACTCTGG AGCACCTGCA GTTGCTATCC AACCAACTTC TCGCACCACC TCTTCCTGAT
    GGAACAATCA GTTCAAGTTC CATTCTTTTG GCTCAGTCAC TGCAACACTG CATCCATTCC
    CAGAACTGTG CTGCCACAGA CCTCTTCTAC CAGGGCAATT CACAAGCCAT CAGAGAGTGG
    CTTACTGTTG CCATCACTCG GACACTGCAT CAAGGAGAGG AGAGCCTGTT AGACCTCACT
    AAGCAGATCT GCTCTTTTTT GCAGACTGCA CCAGAACAGT TTCCTGCTGA AGAATTCCCA
    ATTTCAGAAT CCAAAGTCAA CATGGATGTT AATTTTCCTG GGGCTGCGTT TGTAATTGTG
    TCCTGTAAAG AAAGCCAGTC TGGATTCCGC AAAGATTCTT CCCTGTATAA AGCTCCTTGG
    GCTCGGGTGC TTGTGTACGG GCTGGGGCAC AAAGTTAAGA GGAATGGCCA GTTAAACCTG
    ATTGAGGCAG TGTGTTACCC TCGAGACGCC TCTCCGACCA ACACAGGCCT AACACCTCCC
    CCAACAACCA ATCAGTACCC TTCAGTGATC ACTCCTACAG ACAAAGTGCA CATCAAACTA
    GGAGTGTCTC CTCCTCCTGG AGCTGTGCTG GTCTTGCATT CTCTTCCCCT TGAGTTCCCC
    CTGGCAATGG CATTTACAGA ACAGCTGTTA ACTTGGAAAC TGGAAGATGT GGATGGGAAA
    TCAGAAGATG AACTGGATAC TATTCCTCCT TCAGTTCTTT TGCAAGTAGT GGAATTTTTA
    GGGAACTTTC TGTGGACAAC TGACATGGCA GCATCTGTAA AGGAATTGAT ATTTCATCTC
    TTGGCTGAAT TGCTTCGCAA AATTCACAAC CTGGAGCAGA AGAAAAGTCC TGCAGGATTA
    TCATCTTCTA TTGCCCTTCA GCTGAATCCC TGTCTAGCTA TGCTTATGGC CCTGCAGTCA
    GAACTCCATA AGCTGTACGA TGAGGAAACA CAAAACTGGG TGTCTGGAAA TGCATGCGGT
    GGTTCTGGTG TTGGAGCTGC TGACCAAGGA AGATTTTCAA CATACTTCCA TGCTCTGATG
    GAAGTTTGCC TTGCTGTAGC AGAAGTAACT CTACCTATCA ATATGAGCGT AACAGCTAAT
    GTGATATCTT CAACGAGTGC ACCAAATCTT AGTGACTCTT CCTCCTCATC TTCATCTTCT
    CCAGGACAGA CACCACAGAG TCCAAGCTTA TTGTCAAAGA GGAAAAAAGT CAAAATGAAG
    AGAGAAAAGA CATCGTCTTC AGGCAAACGA TCTTCATCCA GAGCAGCTGA AACGGATGCT
    GCAATCCTCA GTATAGGAGG AAGCAAGCCT GAGGATATGC TATGGTTTCA TAGAGCACTG
    ACTTTGCTTA TCATTCTCAG ACACCTAACG AAGAAGGATC CACAAGGGTT GGGTGTGACA
    AACGATGCTG TAGCAGATGC ATGTCAGGCA CTGGTAGGAC CCACAGCTCA TAGCCGTTTG
    CTTGTCATTT CTGGAATACC AACTCACTTG GAGGAGAGTA CTGTCAGAAA TGCAATCAGG
    AAGGCGTGTA ATGCGCATGG TGGAGTCTTC AAGGATGAAA TCTACATCCC TCTGCAGGAA
    GAGGATCCCA AAAAAATCAA GGACAAAGCA GAAGGAGGAG AATGCAGAAC TGAGCTGGAA
    AAGCCAACTG TTTCAAGTAG TGCGGACAGT CTGGATATCA GCAGCTCTAG CAGTGTCACA
    CCTGCTATGA GCGTTAGTGC GTCAGCATCA ACGAGCCAGG CTTCCCTGTG CAGCTCACAG
    GGTATCTCCC GGACTGTTAG TGACATCTCT GTTGATCAGT ACCAGGCTGG GCTGGAACTG
    GCCATCCCAC CAGGACTGCT GGAGCCACAC GTTTCCAGCC AGGAGAGCTT GGATCTGTCG
    CACTGCAGTA CTGGGAGCCT TGGCAGCCTT GGCAGCCTCG GGGAGCCGCT TGACAATGTA
    GAGACGGCGT CAGTGTCTGA TGTCGGGTCG ATGTACACAG TCACATCTCT GGACAACCAG
    CAACTTCTGT CACGGCCAAT CAAAGGTTTT GCTGTGGTGG AGATACGATC AAGAGCTAAA
    ATAGAGAAAA TCCGAGCCAG CCTATTTAAC AGTAGTGATC TCATTGGTTT GTCAAGTCTG
    GATGGTGAAG ACGAACTGAT GGAAATGTCA ACTGAAGAGA TTTTAACTGT TTCTACTGTA
    AATCAGAGTT TATTTGACAC ACAAGGGAGC CCGGCGCTAG AAGACTACTT CAATGATAAG
    TCAATAAAAG GTGAGAAATT GGTTCCTGGT GCTCGGGAGG TCCTAACAGA AATATTTAAA
    AGCTGTGTCC ATTCTGAGCA AATGCTGAGC CTGACTCCAA CAAAACCAAT CAAGGTTGCT
    GATATCTATC TTAGTAAAGA GCAGATAAAT TCTCAGACTC CTGGAAACCT TCTTCATGTC
    TTCTTCACAA ATGTCCGTCC TCCAAAGAAG GTGCTGGAGG ACCAGCTTAC TCAGATTCTA
    AGGAAATACG GTGTGCCCAA GCCGAAATTT GACAAGAGCA AATACAACAA GGCAGGCAAA
    GAACAGCATC CGGTGAAGGT TGTGAGCACC AAGCGCCCAG TTACAAAACC ACCCACAAAA
    GAAAAGTCTG TACTCAACAG CGTCAGCCGA ACTGCTTTGA GTGAGAAGAA ACCTACTGTA
    AAGCCAAAGT CTCCTGAGAA GAGCAAACCT GAAGAGAAGG ACCCTGAAAA GTCTCCCACA
    AAGAAACAAG AAGTTCCTGA GGAGAAGTAC CTGACACTGG ATGGATTTCA CAGATTTGTC
    ATTGACCGAT CTAAGCAGGA TATCCGTAGT GTGTGGAGAG CTATTCTGTC CTGTGGATAT
    GATCTGCATT TTGAAAGATG TGCCTGCATT GATGCAAGAC ATGCACAGAA AGCATCTCGG
    AAGTGGACTC TGGAAATGGA CGTGGCTCTC GTTCAGTACA TTAACAGGCT TTGTCGTCAC
    CTCGCAATTA CACCAGCACG ACTCCATCCA CATGAAGTAT ATTTGGATCC AGCTGATGCA
    GCAGATCCTA GAATCTCCTG TCTGTTGAAT GTTCCCGTTG AAAGTCTCCG CCTGCGGTTT
    GCACTGCTTC AGTCTCTCAA TACGACGTTA GAGACGTTTT TCTTACCGCT GGTAGAGCTT
    CGGCAGACTG AGATGTACAG TAACAGCATT GCTGCACTGC TGCAGGAGGC CAAAGGTTTA
    ATCTTCTATG ACACAAAAAT AACCGTAATG AACAGAGTGC TGAATGCCAC TGTCCAAAGA
    ACTGCTGATC ACGCAGCCCC AGAAATCACC TTGGATCCCT TGGAAATAGT TGGAGGGGAG
    ATCCGTTCTT CAGAAAATTC CTACTTCTGC CAGGCAGCAA GGCAGCTGGC CTGTGTGCCA
    TCCTCACAGC TCTGCGTCAA ACTCGCCAGT GGTGGGGACC CTACGTACGC GTTCAACATC
    CGGTTCACAG GGGAGGAGGT CCATGGAACA AGTGGATCTT TCCGTCACTT CCTCTGGCAA
    GTTTGTAAGG AGCTGCAGAG CTCCTCACTC TCCCTTCTCC TGTTGTGTCC AAGCTCTGCG
    GTCAACAAGA ACAAGGGGAA GTACATTTTG ACACCTAGCC CCATAACCTA TGCAGAGGAG
    CAGCTGTTCC ATTTCTTTGG GCAGCTTCTG GGCATTGCAA TTCGAGCAGA TGTTCCCCTG
    CCACTCGACC TCCTGCCCTC ATTCTGGAAG ACTCTGGTAG GAGAGCCGCT GGATCCTGAT
    GTTGATCTCC AGGAAGCTGA TATCCTCACC TACAACTACA TTAAAAAGTT TGAAAATATA
    AATGATGAAA CTGAACTGGA AGCTCTGTGT GCAGAAATTG CTTCTCAGCA TTTAGCAACA
    GAGAGCCCTG ACTGTCCTAA TAAGCCATGC TGCAAATTCA CCTACCTGAC CATGACAGGG
    GAAGAGGTGG AACTCTGTCC CAGGGGCAGG CACGTCCCAG TGGGATGGGA GAACAAGGAC
    GTGTATGCAG CTGCAATCAG GAGCTTGAGA ATGCGTGAGC TGCAGACTCC AGAGTGCATG
    ACTGCAGTGC GTGCTGGGCT TGGGTCCATC ATTCCCCTGC AGCTTCTGAC CACGCTGACC
    CCTCTTGAGA TGGAGCTGAG GACATGTGGG CTTCCATACA TCAACCTGGA GTTTCTCAAG
    GCGCACACGA TGTACCAGGT GGGACTGATG GAAACTGATC AGCACATTGA GTTTTTTTGG
    AGCGCGCTGG AGATGTTCAC TCAGGAGGAG CTCTGCAAGT TCATAAAGTT TGCCTGTAAC
    CAGGAGCGGA TCCCCTTCAC GTGTCCTTGC AAGGACGGCG GTCCCGATAC CGCCCACGTC
    CCGCCTTACC CCATGAAAAT AGCCCCCCCT GATGGAGCTG CAGGTTCTCC GGACTCTCGG
    TACATCCGTG TGGAAACGTG CATGTTCATG ATCAAACTTC CTCAGTACTC ATCACTGGAC
    ATCATGCTGG AGAAGCTCCG ATACGCCATC CACTACCGGG AGGATCCTCT CAGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42923
    Clone ID Related Accession (Same CDS sequence) XM_015275539.1
    Accession Version XM_015275539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
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    5521
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    5881
    5941
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    6181
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    6301
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    6421
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    7141
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    9301
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    9721
    9781
    9841
    9901
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    10021
    10081
    10141
    10201
    10261
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    10381
    10441
    10501
    10561
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    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TCTAACAGTG TATTTTATGT TTTGAAGTTG
    GTGATCTATG CTGGTCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT AGTAAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGGAGTG GTCGTGAGCA CAACACTCCA
    GACAAAGCCA TGTGGGGCTT TGCTTGCACT GTTCGAGCAC AGGAGTCTTC AGAGGATGTG
    TCAGGAGGCC TGCCATTCTT AGTTGACCTG GCACTTGGCC TTTCTGTGTT AGCTTGTTCT
    ATGCTTCGGA TTTTATACAA TGGACCAGAA ATTACCAAAG ATGAAGAAAC CTGTCAAGAG
    CTTCTAAGAT CTAAACTATT ACAAAGGTGC CAATGGCAGG TGGAAGCTAA TGGTGTAATA
    TCTCCAGCCC TTACACCGAG TCCTTCTCCA CTGCCTCTTA CTATAGATGA GGACAGAGAA
    TTTACTTATC CTTCTGATGT GCTGGTGCCT CCCATTGGAC ACTATTTTGA TCTGCCTAGG
    ATTAGGCTGC CTCCAGGAAT CATGATAAAA CTCAGGGAAA TTTCTGGACG TGCTCGGCCT
    CAATTTAGAC CTAGCATAAA GGAAGTGATT CAGCCCGAGG TAATGGAGGA GATGGTAGTT
    TCATGTGTGA TTAAACATTT GAATTTGGTT GATGCACTCC AGTCCCTGAT AAACTTCCAG
    TATCAGGAAG AACATGCTGA AGAATATGAT TTACTTTGTA AAATTATGGG AGAGACTTTT
    AAGAAGCTAA ATGCCATGGA GAGACAATTA CAGAGTGTGG CTGAGCTGGA ACAGAAATGG
    CAGAGTGAGG TTGATGACGC CATGCATGGG AAGCTGGAGA ATAATGTTCC CTTCTTCTAT
    GATTACCACT TCAATGAGAG CAAGATGAAA GAACTGGAAC TTCTGTGTTC TATGAAGGAA
    GTTTCTTTTG ATGGAAGTGA TCTTGAGAAT GTGGTCCTGG CATTAAGGGA GAAGTTCTTT
    CAAGAGGTGA ATTCACTGAT ACAGAAGACT TATCATCCCC TAGCGAAAAC AAAAGCATTA
    GTTAAAAGCT TGATGAACAG GGCAGAGCTG TTGCTGCATG TCACTATTGC AGCGCAGTCT
    GGTATCACAA GAAGTATATC TGGAACCCCT GCAGAGACTC CAGCCTGTAA GTCTGCATCT
    GAAACAAAAG TGATATCTCA TGCAGTGCGC CAGCCCGTCT TCCTTCGCAG TATGTCAGCA
    CCATCTGACT TAGAGATGAT TGGTAACGAA GATATTGAGT TTGCTCGATC AAGTCAAAGG
    CGACGCCATG TTACCAGCCA CAGAAGTAGC TCTTTCACTC TTCTGCAATC ACTGACCATT
    GAGGACAGCA GAGATAAACC TACATACAGT GTCCTACTGG GGCAGCTCTT TGCTTTCATT
    GGAACTAATC CTGACCAAGC GGTATCCAGC AGCAGTTTTC TGCTCGCTGC TCAGACAAGG
    TGGCGGCGTG GGAACACAAG GAAGCAAGCC TTAGTGCATA TGAGAGAGCT GCTGACTGCG
    GCTGTTCGGG TTGGTGGGGT GACACACCTC GTGGGCCCTG TGACCATGGT ACTTCAGGGA
    GGACCAAGAA TTGAAGAGCT CACGTGTGGT GGGATGGTGG AACAAGTCCA GGAAGCTTTT
    GGGGAGACTA TGACATCCGT GGTCTCGCTG TGTGCTCGCT ACCCCATTGC TTGTGCAAAC
    AGCATTGGCC TCTTATGCAC TATACCTTAC ACAAGGAGTG AAGAGAAGTG TTTGGTACGT
    AGTGGCCTGG TACAGCTTAT GGACAGGCTG TGCAGCTTAA GCAGCCAGGC AGAATCCAGT
    TCAAATGAAA AACAGACCAA GAAGCAGAAG GTGGCCACCA TGGCTTGGGC TGCCTTTCAG
    GTGCTGGCCA ATCGCTGTGT TGAATGGGAG AAAGAAGAAG GTGGTTCAAC AGAAGCTGTT
    CACTCTGGTC TGGCTCGTCA GGTCTCCAGC CTTCTTACCA ATCACCTTGC ACGAGCTACA
    GAGTGTTGTG GTAATCAAGC TGCAGGAAAT GATGCACTTC AAGACGTCCT CAGTCTTCTT
    AATGACCTGT CGAGGAGTCA CATAGGTAAA GCAATCCTGA GCCAGCCAGC CTGTGTGTCC
    AAACTTCTGT CACTTCTCTT GGATCAACGT CCCTCTCCAA AGTTGGTTCT TATAATCCTT
    CAGTTGTGTC GTGCTGCTCT ACCTCTCATG AGTGTTGAAG ACTGTGGAAA TGTAGAACTG
    CCTCCCTGGA GTTACTCTGT GCCCTCCCTG AACAGTGAAC AAGATGATCC CAGCGACCCA
    GCCTCCAAGA TTGCATCTCT GCTCTTAGCA AAGCTGGCAG ATTATGTAGT ACCAGGATGC
    CAGACAGTTC TTTCTCCAAC TGCTTCTGAA CCAGATACTG CTTTAGCAAA AAGCAGCCCT
    AAGAATTCTA TAAAAGGCGA TAAAGACCCT GGAGAAGAAA GTGAGGCAGT GGATGGCAAA
    CTTTCTATTT TCATCCATAA ACGGGAAGAC CAGTCATCCC ACGAAGTGCT TCAGCCATTG
    CTGAGTAGCT CCGAAGGTCG CCCATTCCGA CTAGGAACTG GAGCCAACAT GGAGAAGGTG
    GTGAAAATGG ATCGTGACAT GACTAAAAGT GGCTGCTGTG AAGTTATCAC AGAAGAAGCT
    GCTGCAGCTC TTCGCAAGGC AACTAAGTGG GCTCAGTCTG GTCTGATAGT TAGTGTTGGG
    CCACCCATAG AAACTGTCAA TCCAGAAGCA ACCAGTGGGA TGTCCTCTGG GGACAAAAAG
    AAGACTGCAC AGACATCTAT TTGCAGAGAG AGAAATTCTG AGCTTGCCAG GACTGATCCT
    GTAAGACCAT TCATCAGTGG TCATGTTGCA AATAGCATGG CAGCAGAAGT GATCGCTTTG
    CTACATAGCT TGTTGATGGC ACCAGAATCA AATGCGGCTC AAATATGGAC AACAACTGCT
    GAAAAAGTTC TGTCTCGGGC TCTGATGTAC ATTCCACAAC TCGGGAAATA CGCAGAGAGT
    ATTTTGGAGA ATGGAAGCAG CAGTGGAAGG AAGCTTGCTA AACTTCAAAG AATTGCTCGA
    CAGGCAGTAG CTGCTCTTTG TGCTCTAGGT GGCTTTAAGG AGACAATTAA AATCGGTTCT
    GAAGTTCAGG TTTTAGGTAA AGGAATAGCA GGAAGCATTG GAGTTGTGGC ATCTATTAAT
    GAACAAGAAG GTATAGCAAC GGTCAAATTT CCGCCTACCA CTATAGACAG TAGAAAGACC
    TCACAAGCAT CAGACACTTT AACTATTCCG TTATCTAGGC TCTGTGTTCC AAGATCTGAG
    GCATTGCCTC TTCATAAACT GTCCATTACT GAGAAGGTAG TACAGGCAGT CCAGTCCATG
    TTGCTTCCTC AGGAGGGAAG TCTATCTATT CACACCTCAC TTCCTGCAAC AGGAGATGGC
    TCAACTCCAG TAATGGCAGT GGTCCGGCTC CTTGCTGAAA TAAGAACCAG AGCATGCCTG
    GTGATGGCTC AGCTGTTAGA AGACAGTTCA TTCTGTGAAG AATTCATTCA ACAGTGCCCA
    GCTGCTGTAG AGGTTCTTAA TTTGGTAGCC CAGGAGTGCA GCCCTGGAGA GAGGCTCACT
    GTAGTAGAAA TGCAGTGTGA AAGGTTAAGG ATGTTGTATC GTGACTGTGC TCGACCCCCA
    CCTCCTCCAC TGCAGGCAGA CAGAAGGCAG CCCAAAGAAA TCACTTGGAG CCCATCAAGA
    GTGTTTCCCC CAGTACGAGC ATGCATGTTT TCATCACATC TTGCTGCTGT GACCTTCCTG
    GCTGATCCAA GTGCAGGTGG GGGACTTCCT CGGGGAACGT TCATTTATGC CACTTCTCCA
    GTTCCCGTGC AGGCACCATC ATTCTACTGG GAAATTGAAA TAGTTTCCTA TGGAGATACA
    GATGATGACA CTGGTCCAAT AGTTTCATTT GGATTCGCTA CAGAGGCAGA AAAACGGGAT
    GGGGCTTGGA CAAACCCAGT GGGCACCTGT CTTTTTCATA ACAATGGGCG AGCAGTGCAT
    TACAATGGCT CCAGCTTGTT ACAGTGGAAG AGTGTTCGAT TGGATGTGAC TCTTTCTCCT
    GGTGATGTGG CTGGAATTGG ATGGGAAAGA GCAGAAGGAA CTCCACCCCC ACCAGGGCAG
    CCTGCTAAAG GCAGAGTTTA TTTTACTTAT TGCGGGCAGC GACTTGGGCC ATACCTAGAG
    GATGTCTCTG GTGGAATGTG GCCAATTGTT CACATACAGA AAAAGAACAC AAAAGTCCGT
    GCTAATTTTG GATCTCGCCA GTTTGCCTAT GCCGAAGGCC AAGCTCACAG GAATGCTGCT
    GATCTGTGTA TTGACCTAGC TGAAGAGATA AGTGCCAACT TTGAGGCATT GCCATTTGCT
    ATGGCTTCTG ATAGCGATAA CGACGCTGGC ACCAGCATAG CTTCTGATCC TGGGACTCAC
    GGACCTCCCT GTAGAATTGC TGCTGTTGCT ACGGCTCAAC AACAATATGA CAGTGACACA
    TCTTGTCACT ATAAAATGGA ACTAAGCTAT GAGAACTTCA TCACATCAGG GCCTGATCCT
    CATCCCCCTC CTATTGCAGA TGATGAAAGT GATGATGACG ACGATGATGA TATTCCCAGG
    GAGGACCACT ATGCCCTGCT GGTTAAAGCC TGGGAAACTA AAGTTTTCCC TACAATTAGA
    AGAAGATTCC GTAATGAGGC TGAGAGGAAA TCTGGGCTGG ATCAAATTAA AGGAGCCTTA
    CAGTTAGGAA TGGTTGATAT AGCACGACAA ACTGTAGAAT TCTTGTATGA GGAAAACGGT
    GGCATCCCGA GAGACCTCTA CCTTCCTACC ATTGAGGATA TCAAAGATGA AGCAAACAAG
    TTTACAATCG ATAAAGTACG AAAAGGTCTC ACTGTTGTAA CTCGCTCTCC TGACAGTAAC
    AACGTTACCA GCAGTGCTGT TGGAACAGCT TTACCCAAGT TTGCTATTCG GGGAATGCTG
    AAGACATTTG GTCTTCATGG TGTTGTCCTG GATGTTGATT CAGTGAATGA ATTGGTACAA
    GTAGAGACCT ATCTCAGAAG TGAGGGAGTT CTGGTAAGAT ATTGGTATCC TATTGACATG
    TTAGAAAGGC CACCTGCTGG TTATAGGAGG ACTGCAACAA ATGGATTGGT TACCCTGGAC
    AATACCAATA TCCAAATCCA CAGAGAGCTT TTGAGAAGTG AAGCTGCACT GGCCAAGCTG
    TACTGCCGCA TGGCTCTCCT CAATATCTTT GCCCCCAAAT CTCCACATAT GTTCACCCGT
    CTGTTTCATA TCCCCGCTAT CCGTGATATC ACTCTGGAGC ACCTGCAGTT GCTATCCAAC
    CAACTTCTCG CACCACCTCT TCCTGATGGA ACAATCAGTT CAAGTTCCAT TCTTTTGGCT
    CAGTCACTGC AACACTGCAT CCATTCCCAG AACTGTGCTG CCACAGACCT CTTCTACCAG
    GGCAATTCAC AAGCCATCAG AGAGTGGCTT ACTGTTGCCA TCACTCGGAC ACTGCATCAA
    GGAGAGGAGA GCCTGTTAGA CCTCACTAAG CAGATCTGCT CTTTTTTGCA GACTGCACCA
    GAACAGTTTC CTGCTGAAGA ATTCCCAATT TCAGAATCCA AAGTCAACAT GGATGTTAAT
    TTTCCTGGGG CTGCGTTTGT AATTGTGTCC TGTAAAGAAA GCCAGTCTGG ATTCCGCAAA
    GATTCTTCCC TGTATAAAGC TCCTTGGGCT CGGGTGCTTG TGTACGGGCT GGGGCACAAA
    GTTAAGAGGA ATGGCCAGTT AAACCTGATT GAGGCAGTGT GTTACCCTCG AGACGCCTCT
    CCGACCAACA CAGGCCTAAC ACCTCCCCCA ACAACCAATC AGTACCCTTC AGTGATCACT
    CCTACAGACA AAGTGCACAT CAAACTAGGA GTGTCTCCTC CTCCTGGAGC TGTGCTGGTC
    TTGCATTCTC TTCCCCTTGA GTTCCCCCTG GCAATGGCAT TTACAGAACA GCTGTTAACT
    TGGAAACTGG AAGATGTGGA TGGGAAATCA GAAGATGAAC TGGATACTAT TCCTCCTTCA
    GTTCTTTTGC AAGTAGTGGA ATTTTTAGGG AACTTTCTGT GGACAACTGA CATGGCAGCA
    TCTGTAAAGG AATTGATATT TCATCTCTTG GCTGAATTGC TTCGCAAAAT TCACAACCTG
    GAGCAGAAGA AAAGTCCTGC AGGATTATCA TCTTCTATTG CCCTTCAGCT GAATCCCTGT
    CTAGCTATGC TTATGGCCCT GCAGTCAGAA CTCCATAAGC TGTACGATGA GGAAACACAA
    AACTGGGTGT CTGGAAATGC ATGCGGTGGT TCTGGTGTTG GAGCTGCTGA CCAAGGAAGA
    TTTTCAACAT ACTTCCATGC TCTGATGGAA GTTTGCCTTG CTGTAGCAGA AGTAACTCTA
    CCTATCAATA TGAGCGTAAC AGCTAATGTG ATATCTTCAA CGAGTGCACC AAATCTTAGT
    GACTCTTCCT CCTCATCTTC ATCTTCTCCA GGACAGACAC CGCAGAGTCC AAGCTTATTG
    TCAAAGAGGA AAAAAGTCAA AATGAAGAGA GAAAAGACAT CGTCTTCAGG CAAACGATCT
    TCATCCAGAG CAGCTGAAAC GGATGCTGCA ATCCTCAGTA TAGGAGGAAG CAAGCCTGAG
    GATATGCTAT GGTTTCATAG AGCACTGACT TTGCTTATCA TTCTCAGACA CCTAACGAAA
    AAGGATCCGC AAGGGTTGGG TGTGACAAAC GATGCTGTAG CAGATGCATG TCAGGCACTG
    GTAGGACCCA CAGCTCATAG CCGTTTGCTT GTCATTTCTG GAATACCAAC TCACTTGGAG
    GAGAGTACTG TCAGAAATGC AATCAGGAAG GCGTGTAATG CGCATGGTGG AGTCTTCAAG
    GATGAAATCT ACATCCCTCT GCAGGAAGAG GATCCCAAAA AAATCAAGGA CAAAGCAGAA
    GGAGGAGAAT GCAGAACTGA GCTGGAAAAG CCAACTGTTT CAAGTAGTGC GGACAGTCTG
    GATATCAGCA GCTCTAGCAG TGTCACACCT GCTATGAGCG TTAGTGCGTC AGCATCAACG
    AGCCAGGCTT CCCTGTGCAG CTCACAGGGT ATCTCCCGGA CTGTTAGTGA CATCTCTGTT
    GATCAGTACC AGGCTGGGCT GGAACTGGCC ATCCCACCAG GACTGCTGGA GCCACACGTT
    TCCAGCCAGG AGAGCTTGGA TCTGTCGCAC TGCAGTACTG GGAGCCTTGG CAGCCTTGGC
    AGCCTCGGGG AGCCGCTTGA CAATGTAGAG ACAGCATCAG TGTCTGATGT CGGGTCGATG
    TACACGGTCA CATCTCTGGA CAACCAGCAA CTTCTGTCAC GGCCTATCAA AGGTTTTGCT
    GTGGTGGAGA TACGATCAAG AGCTAAAATA GAGAAAATCC GAGCCAGCCT ATTTAACAGT
    AGTGATCTCA TTGGTTTGTC AAGTCTGGAT GGTGAAGACG AACTGATGGA AATGTCAACT
    GAAGAGATTT TAACTGTTTC TACTGTAAAT CAGAGTTTAT TTGACACACA AGGGAGCCCG
    GCGCTAGAAG ACTACTTCAA TGATAAGTCA ATAAAAGGTG AGAAATTGGT TCCTGGTGCT
    CGGGAGGTCC TAACAGAAAT ATTTAAAAGC TGTGTCCATT CTGAGCAAAT GCTGAGCCTG
    ACTCCAACAA AACCAATCAA GGTTGCTGAT ATCTATCTTA GTAAAGAGCA GATAAATTCT
    CAGACTCCTG GAAACCTTCT TCATGTCTTC TTCACAAATG TCCGTCCTCC AAAGAAGGTG
    CTGGAGGACC AGCTTACTCA GATTCTAAGG AAATACGGTG TGCCCAAGCC GAAATTTGAC
    AAGAGCAAAT ACAACAAGGC AGGCAAAGAA CAGCATCCGG TGAAGGTTGT GAGCACCAAG
    CGCCCAGTTA CAAAACCACC CACAAAAGAA AAGTCTGTAC TCAACAGCGT CAGCCGAACT
    GCTTTGAGTG AGAAGAAACC TACTGTAAAG CCAAAGTCTC CTGAGAAGAG CAAACCTGAA
    GAGAAGGACC CTGAAAAGTC TCCCACAAAG AAACAAGAAG TTCCTGAGGA GAAGTACCTG
    ACACTGGATG GATTTCACAG ATTTGTCATT GACCGATCTA AGCAGGATAT CCGTAGTGTG
    TGGAGAGCTA TTCTGTCCTG TGGATATGAT CTGCATTTTG AAAGATGTGC CTGCATTGAT
    GCAAGACATG CACAGAAAGC ATCTCGGAAG TGGACTCTGG AAATGGACGT GGCTCTCGTT
    CAGTACATTA ACAGGCTTTG TCGTCACCTC GCAATTACAC CAGCACGACT CCATCCACAT
    GAAGTATATT TGGATCCAGC TGATGCAGCA GATCCTAGAA TCTCCTGTCT GTTGAATGTT
    CCCGTTGAAA GTCTCCGCCT GCGGTTTGCA CTGCTTCAGT CTCTCAATAC GACGTTAGAG
    ACGTTTTTCT TACCGCTGGT AGAGCTTCGG CAGACTGAGA TGTACAGTAA CAGCATTGCT
    GCGCTGCTGC AGGAGGCCAA AGGTTTAATC TTCTATGACA CAAAAATAAC CGTAATGAAC
    AGAGTGCTGA ATGCCACTGT CCAAAGAACT GCTGATCACG CAGCCCCAGA AATCACCTTG
    GATCCCTTGG AAATAGTTGG AGGGGAGATC CGTTCTTCAG AAAATTCCTA CTTCTGCCAG
    GCAGCAAGGC AGCTGGCCTG TGTGCCATCC TCACAGCTCT GCGTCAAACT CGCCAGTGGT
    GGGGACCCTA CGTACGCGTT CAACATCCGG TTCACAGGGG AGGAGGTCCA TGGAACAAGT
    GGATCTTTCC GTCACTTCCT CTGGCAAGTT TGTAAGGAGC TGCAGAGCTC CTCGCTCTCT
    CTTCTCCTGT TGTGTCCAAG CTCTGCGGTC AACAAGAACA AGGGGAAGTA CATTTTGACA
    CCTAGCCCCA TAACCTATGC AGAGGAGCAG CTGTTCCATT TCTTTGGGCA GCTTCTGGGC
    ATTGCAATTC GAGCAGATGT TCCCCTGCCA CTCGACCTCC TGCCCTCATT CTGGAAGACT
    CTGGTAGGAG AGCCGCTGGA TCCTGATGTT GATCTCCAGG AAGCTGATAT CCTCACCTAC
    AACTACATTA AAAAGTTTGA AAATATAAAT GATGAAACTG AACTGGAAGC TCTGTGTGCA
    GAAATTGCTT CTCAGCATTT AGCAACAGAG AGCCCTGACT GTCCTAATAA GCCATGCTGC
    AAATTCACCT ACCTGACCAT GACAGGGGAA GAGGTGGAAC TCTGTCCCAG GGGCAGGCAC
    GTCCCAGTGG GATGGGAGAA CAAGGACGTG TATGCAGCTG CAATCAGGAG CTTGAGAATG
    CGTGAGCTGC AGACTCCAGA GTGCATGACT GCAGTGCGTG CTGGGCTTGG GTCCATCATT
    CCCCTGCAGC TTCTGACCAC GCTGACCCCT CTTGAGATGG AGCTGAGGAC ATGTGGGCTT
    CCATACATCA ACCTGGAGTT TCTCAAGGCG CACACGATGT ACCAGGTGGG ACTGATGGAA
    ACTGATCAGC ACATTGAGTT TTTTTGGAGC GCGCTGGAGA TGTTCACTCA GGAGGAGCTC
    TGCAAGTTCA TAAAGTTTGC CTGTAACCAG GAGCGGATCC CCTTCACGTG TCCTTGCAAG
    GACGGCGGTC CCGATACCGC CCACGTCCCG CCTTACCCCA TGAAAATAGC CCCCCCTGAT
    GGAGCTGCAG GTTCTCCGGA CTCTCGGTAC ATCCGTGTGG AAACGTGCAT GTTCATGATC
    AAACTTCCTC AGTACTCATC ACTGGACATC ATGCTGGAGA AGCTCCGATA CGCCATCCAC
    TACCGGGAGG ATCCTCTCAG CGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131025.1
    CDS263..13549
    Translation

    Target ORF information:

    RefSeq Version XM_015275539.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TCTAACAGTG TATTTTATGT TTTGAAGTTG
    GTGATCTATG CTGGTCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT AGTAAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGGAGTG GTCGTGAGCA CAACACTCCA
    GACAAAGCCA TGTGGGGCTT TGCTTGCACT GTTCGAGCAC AGGAGTCTTC AGAGGATGTG
    TCAGGAGGCC TGCCATTCTT AGTTGACCTG GCACTTGGCC TTTCTGTGTT AGCTTGTTCT
    ATGCTTCGGA TTTTATACAA TGGACCAGAA ATTACCAAAG ATGAAGAAAC CTGTCAAGAG
    CTTCTAAGAT CTAAACTATT ACAAAGGTGC CAATGGCAGG TGGAAGCTAA TGGTGTAATA
    TCTCCAGCCC TTACACCGAG TCCTTCTCCA CTGCCTCTTA CTATAGATGA GGACAGAGAA
    TTTACTTATC CTTCTGATGT GCTGGTGCCT CCCATTGGAC ACTATTTTGA TCTGCCTAGG
    ATTAGGCTGC CTCCAGGAAT CATGATAAAA CTCAGGGAAA TTTCTGGACG TGCTCGGCCT
    CAATTTAGAC CTAGCATAAA GGAAGTGATT CAGCCCGAGG TAATGGAGGA GATGGTAGTT
    TCATGTGTGA TTAAACATTT GAATTTGGTT GATGCACTCC AGTCCCTGAT AAACTTCCAG
    TATCAGGAAG AACATGCTGA AGAATATGAT TTACTTTGTA AAATTATGGG AGAGACTTTT
    AAGAAGCTAA ATGCCATGGA GAGACAATTA CAGAGTGTGG CTGAGCTGGA ACAGAAATGG
    CAGAGTGAGG TTGATGACGC CATGCATGGG AAGCTGGAGA ATAATGTTCC CTTCTTCTAT
    GATTACCACT TCAATGAGAG CAAGATGAAA GAACTGGAAC TTCTGTGTTC TATGAAGGAA
    GTTTCTTTTG ATGGAAGTGA TCTTGAGAAT GTGGTCCTGG CATTAAGGGA GAAGTTCTTT
    CAAGAGGTGA ATTCACTGAT ACAGAAGACT TATCATCCCC TAGCGAAAAC AAAAGCATTA
    GTTAAAAGCT TGATGAACAG GGCAGAGCTG TTGCTGCATG TCACTATTGC AGCGCAGTCT
    GGTATCACAA GAAGTATATC TGGAACCCCT GCAGAGACTC CAGCCTGTAA GTCTGCATCT
    GAAACAAAAG TGATATCTCA TGCAGTGCGC CAGCCCGTCT TCCTTCGCAG TATGTCAGCA
    CCATCTGACT TAGAGATGAT TGGTAACGAA GATATTGAGT TTGCTCGATC AAGTCAAAGG
    CGACGCCATG TTACCAGCCA CAGAAGTAGC TCTTTCACTC TTCTGCAATC ACTGACCATT
    GAGGACAGCA GAGATAAACC TACATACAGT GTCCTACTGG GGCAGCTCTT TGCTTTCATT
    GGAACTAATC CTGACCAAGC GGTATCCAGC AGCAGTTTTC TGCTCGCTGC TCAGACAAGG
    TGGCGGCGTG GGAACACAAG GAAGCAAGCC TTAGTGCATA TGAGAGAGCT GCTGACTGCG
    GCTGTTCGGG TTGGTGGGGT GACACACCTC GTGGGCCCTG TGACCATGGT ACTTCAGGGA
    GGACCAAGAA TTGAAGAGCT CACGTGTGGT GGGATGGTGG AACAAGTCCA GGAAGCTTTT
    GGGGAGACTA TGACATCCGT GGTCTCGCTG TGTGCTCGCT ACCCCATTGC TTGTGCAAAC
    AGCATTGGCC TCTTATGCAC TATACCTTAC ACAAGGAGTG AAGAGAAGTG TTTGGTACGT
    AGTGGCCTGG TACAGCTTAT GGACAGGCTG TGCAGCTTAA GCAGCCAGGC AGAATCCAGT
    TCAAATGAAA AACAGACCAA GAAGCAGAAG GTGGCCACCA TGGCTTGGGC TGCCTTTCAG
    GTGCTGGCCA ATCGCTGTGT TGAATGGGAG AAAGAAGAAG GTGGTTCAAC AGAAGCTGTT
    CACTCTGGTC TGGCTCGTCA GGTCTCCAGC CTTCTTACCA ATCACCTTGC ACGAGCTACA
    GAGTGTTGTG GTAATCAAGC TGCAGGAAAT GATGCACTTC AAGACGTCCT CAGTCTTCTT
    AATGACCTGT CGAGGAGTCA CATAGGTAAA GCAATCCTGA GCCAGCCAGC CTGTGTGTCC
    AAACTTCTGT CACTTCTCTT GGATCAACGT CCCTCTCCAA AGTTGGTTCT TATAATCCTT
    CAGTTGTGTC GTGCTGCTCT ACCTCTCATG AGTGTTGAAG ACTGTGGAAA TGTAGAACTG
    CCTCCCTGGA GTTACTCTGT GCCCTCCCTG AACAGTGAAC AAGATGATCC CAGCGACCCA
    GCCTCCAAGA TTGCATCTCT GCTCTTAGCA AAGCTGGCAG ATTATGTAGT ACCAGGATGC
    CAGACAGTTC TTTCTCCAAC TGCTTCTGAA CCAGATACTG CTTTAGCAAA AAGCAGCCCT
    AAGAATTCTA TAAAAGGCGA TAAAGACCCT GGAGAAGAAA GTGAGGCAGT GGATGGCAAA
    CTTTCTATTT TCATCCATAA ACGGGAAGAC CAGTCATCCC ACGAAGTGCT TCAGCCATTG
    CTGAGTAGCT CCGAAGGTCG CCCATTCCGA CTAGGAACTG GAGCCAACAT GGAGAAGGTG
    GTGAAAATGG ATCGTGACAT GACTAAAAGT GGCTGCTGTG AAGTTATCAC AGAAGAAGCT
    GCTGCAGCTC TTCGCAAGGC AACTAAGTGG GCTCAGTCTG GTCTGATAGT TAGTGTTGGG
    CCACCCATAG AAACTGTCAA TCCAGAAGCA ACCAGTGGGA TGTCCTCTGG GGACAAAAAG
    AAGACTGCAC AGACATCTAT TTGCAGAGAG AGAAATTCTG AGCTTGCCAG GACTGATCCT
    GTAAGACCAT TCATCAGTGG TCATGTTGCA AATAGCATGG CAGCAGAAGT GATCGCTTTG
    CTACATAGCT TGTTGATGGC ACCAGAATCA AATGCGGCTC AAATATGGAC AACAACTGCT
    GAAAAAGTTC TGTCTCGGGC TCTGATGTAC ATTCCACAAC TCGGGAAATA CGCAGAGAGT
    ATTTTGGAGA ATGGAAGCAG CAGTGGAAGG AAGCTTGCTA AACTTCAAAG AATTGCTCGA
    CAGGCAGTAG CTGCTCTTTG TGCTCTAGGT GGCTTTAAGG AGACAATTAA AATCGGTTCT
    GAAGTTCAGG TTTTAGGTAA AGGAATAGCA GGAAGCATTG GAGTTGTGGC ATCTATTAAT
    GAACAAGAAG GTATAGCAAC GGTCAAATTT CCGCCTACCA CTATAGACAG TAGAAAGACC
    TCACAAGCAT CAGACACTTT AACTATTCCG TTATCTAGGC TCTGTGTTCC AAGATCTGAG
    GCATTGCCTC TTCATAAACT GTCCATTACT GAGAAGGTAG TACAGGCAGT CCAGTCCATG
    TTGCTTCCTC AGGAGGGAAG TCTATCTATT CACACCTCAC TTCCTGCAAC AGGAGATGGC
    TCAACTCCAG TAATGGCAGT GGTCCGGCTC CTTGCTGAAA TAAGAACCAG AGCATGCCTG
    GTGATGGCTC AGCTGTTAGA AGACAGTTCA TTCTGTGAAG AATTCATTCA ACAGTGCCCA
    GCTGCTGTAG AGGTTCTTAA TTTGGTAGCC CAGGAGTGCA GCCCTGGAGA GAGGCTCACT
    GTAGTAGAAA TGCAGTGTGA AAGGTTAAGG ATGTTGTATC GTGACTGTGC TCGACCCCCA
    CCTCCTCCAC TGCAGGCAGA CAGAAGGCAG CCCAAAGAAA TCACTTGGAG CCCATCAAGA
    GTGTTTCCCC CAGTACGAGC ATGCATGTTT TCATCACATC TTGCTGCTGT GACCTTCCTG
    GCTGATCCAA GTGCAGGTGG GGGACTTCCT CGGGGAACGT TCATTTATGC CACTTCTCCA
    GTTCCCGTGC AGGCACCATC ATTCTACTGG GAAATTGAAA TAGTTTCCTA TGGAGATACA
    GATGATGACA CTGGTCCAAT AGTTTCATTT GGATTCGCTA CAGAGGCAGA AAAACGGGAT
    GGGGCTTGGA CAAACCCAGT GGGCACCTGT CTTTTTCATA ACAATGGGCG AGCAGTGCAT
    TACAATGGCT CCAGCTTGTT ACAGTGGAAG AGTGTTCGAT TGGATGTGAC TCTTTCTCCT
    GGTGATGTGG CTGGAATTGG ATGGGAAAGA GCAGAAGGAA CTCCACCCCC ACCAGGGCAG
    CCTGCTAAAG GCAGAGTTTA TTTTACTTAT TGCGGGCAGC GACTTGGGCC ATACCTAGAG
    GATGTCTCTG GTGGAATGTG GCCAATTGTT CACATACAGA AAAAGAACAC AAAAGTCCGT
    GCTAATTTTG GATCTCGCCA GTTTGCCTAT GCCGAAGGCC AAGCTCACAG GAATGCTGCT
    GATCTGTGTA TTGACCTAGC TGAAGAGATA AGTGCCAACT TTGAGGCATT GCCATTTGCT
    ATGGCTTCTG ATAGCGATAA CGACGCTGGC ACCAGCATAG CTTCTGATCC TGGGACTCAC
    GGACCTCCCT GTAGAATTGC TGCTGTTGCT ACGGCTCAAC AACAATATGA CAGTGACACA
    TCTTGTCACT ATAAAATGGA ACTAAGCTAT GAGAACTTCA TCACATCAGG GCCTGATCCT
    CATCCCCCTC CTATTGCAGA TGATGAAAGT GATGATGACG ACGATGATGA TATTCCCAGG
    GAGGACCACT ATGCCCTGCT GGTTAAAGCC TGGGAAACTA AAGTTTTCCC TACAATTAGA
    AGAAGATTCC GTAATGAGGC TGAGAGGAAA TCTGGGCTGG ATCAAATTAA AGGAGCCTTA
    CAGTTAGGAA TGGTTGATAT AGCACGACAA ACTGTAGAAT TCTTGTATGA GGAAAACGGT
    GGCATCCCGA GAGACCTCTA CCTTCCTACC ATTGAGGATA TCAAAGATGA AGCAAACAAG
    TTTACAATCG ATAAAGTACG AAAAGGTCTC ACTGTTGTAA CTCGCTCTCC TGACAGTAAC
    AACGTTACCA GCAGTGCTGT TGGAACAGCT TTACCCAAGT TTGCTATTCG GGGAATGCTG
    AAGACATTTG GTCTTCATGG TGTTGTCCTG GATGTTGATT CAGTGAATGA ATTGGTACAA
    GTAGAGACCT ATCTCAGAAG TGAGGGAGTT CTGGTAAGAT ATTGGTATCC TATTGACATG
    TTAGAAAGGC CACCTGCTGG TTATAGGAGG ACTGCAACAA ATGGATTGGT TACCCTGGAC
    AATACCAATA TCCAAATCCA CAGAGAGCTT TTGAGAAGTG AAGCTGCACT GGCCAAGCTG
    TACTGCCGCA TGGCTCTCCT CAATATCTTT GCCCCCAAAT CTCCACATAT GTTCACCCGT
    CTGTTTCATA TCCCCGCTAT CCGTGATATC ACTCTGGAGC ACCTGCAGTT GCTATCCAAC
    CAACTTCTCG CACCACCTCT TCCTGATGGA ACAATCAGTT CAAGTTCCAT TCTTTTGGCT
    CAGTCACTGC AACACTGCAT CCATTCCCAG AACTGTGCTG CCACAGACCT CTTCTACCAG
    GGCAATTCAC AAGCCATCAG AGAGTGGCTT ACTGTTGCCA TCACTCGGAC ACTGCATCAA
    GGAGAGGAGA GCCTGTTAGA CCTCACTAAG CAGATCTGCT CTTTTTTGCA GACTGCACCA
    GAACAGTTTC CTGCTGAAGA ATTCCCAATT TCAGAATCCA AAGTCAACAT GGATGTTAAT
    TTTCCTGGGG CTGCGTTTGT AATTGTGTCC TGTAAAGAAA GCCAGTCTGG ATTCCGCAAA
    GATTCTTCCC TGTATAAAGC TCCTTGGGCT CGGGTGCTTG TGTACGGGCT GGGGCACAAA
    GTTAAGAGGA ATGGCCAGTT AAACCTGATT GAGGCAGTGT GTTACCCTCG AGACGCCTCT
    CCGACCAACA CAGGCCTAAC ACCTCCCCCA ACAACCAATC AGTACCCTTC AGTGATCACT
    CCTACAGACA AAGTGCACAT CAAACTAGGA GTGTCTCCTC CTCCTGGAGC TGTGCTGGTC
    TTGCATTCTC TTCCCCTTGA GTTCCCCCTG GCAATGGCAT TTACAGAACA GCTGTTAACT
    TGGAAACTGG AAGATGTGGA TGGGAAATCA GAAGATGAAC TGGATACTAT TCCTCCTTCA
    GTTCTTTTGC AAGTAGTGGA ATTTTTAGGG AACTTTCTGT GGACAACTGA CATGGCAGCA
    TCTGTAAAGG AATTGATATT TCATCTCTTG GCTGAATTGC TTCGCAAAAT TCACAACCTG
    GAGCAGAAGA AAAGTCCTGC AGGATTATCA TCTTCTATTG CCCTTCAGCT GAATCCCTGT
    CTAGCTATGC TTATGGCCCT GCAGTCAGAA CTCCATAAGC TGTACGATGA GGAAACACAA
    AACTGGGTGT CTGGAAATGC ATGCGGTGGT TCTGGTGTTG GAGCTGCTGA CCAAGGAAGA
    TTTTCAACAT ACTTCCATGC TCTGATGGAA GTTTGCCTTG CTGTAGCAGA AGTAACTCTA
    CCTATCAATA TGAGCGTAAC AGCTAATGTG ATATCTTCAA CGAGTGCACC AAATCTTAGT
    GACTCTTCCT CCTCATCTTC ATCTTCTCCA GGACAGACAC CGCAGAGTCC AAGCTTATTG
    TCAAAGAGGA AAAAAGTCAA AATGAAGAGA GAAAAGACAT CGTCTTCAGG CAAACGATCT
    TCATCCAGAG CAGCTGAAAC GGATGCTGCA ATCCTCAGTA TAGGAGGAAG CAAGCCTGAG
    GATATGCTAT GGTTTCATAG AGCACTGACT TTGCTTATCA TTCTCAGACA CCTAACGAAA
    AAGGATCCGC AAGGGTTGGG TGTGACAAAC GATGCTGTAG CAGATGCATG TCAGGCACTG
    GTAGGACCCA CAGCTCATAG CCGTTTGCTT GTCATTTCTG GAATACCAAC TCACTTGGAG
    GAGAGTACTG TCAGAAATGC AATCAGGAAG GCGTGTAATG CGCATGGTGG AGTCTTCAAG
    GATGAAATCT ACATCCCTCT GCAGGAAGAG GATCCCAAAA AAATCAAGGA CAAAGCAGAA
    GGAGGAGAAT GCAGAACTGA GCTGGAAAAG CCAACTGTTT CAAGTAGTGC GGACAGTCTG
    GATATCAGCA GCTCTAGCAG TGTCACACCT GCTATGAGCG TTAGTGCGTC AGCATCAACG
    AGCCAGGCTT CCCTGTGCAG CTCACAGGGT ATCTCCCGGA CTGTTAGTGA CATCTCTGTT
    GATCAGTACC AGGCTGGGCT GGAACTGGCC ATCCCACCAG GACTGCTGGA GCCACACGTT
    TCCAGCCAGG AGAGCTTGGA TCTGTCGCAC TGCAGTACTG GGAGCCTTGG CAGCCTTGGC
    AGCCTCGGGG AGCCGCTTGA CAATGTAGAG ACAGCATCAG TGTCTGATGT CGGGTCGATG
    TACACGGTCA CATCTCTGGA CAACCAGCAA CTTCTGTCAC GGCCTATCAA AGGTTTTGCT
    GTGGTGGAGA TACGATCAAG AGCTAAAATA GAGAAAATCC GAGCCAGCCT ATTTAACAGT
    AGTGATCTCA TTGGTTTGTC AAGTCTGGAT GGTGAAGACG AACTGATGGA AATGTCAACT
    GAAGAGATTT TAACTGTTTC TACTGTAAAT CAGAGTTTAT TTGACACACA AGGGAGCCCG
    GCGCTAGAAG ACTACTTCAA TGATAAGTCA ATAAAAGGTG AGAAATTGGT TCCTGGTGCT
    CGGGAGGTCC TAACAGAAAT ATTTAAAAGC TGTGTCCATT CTGAGCAAAT GCTGAGCCTG
    ACTCCAACAA AACCAATCAA GGTTGCTGAT ATCTATCTTA GTAAAGAGCA GATAAATTCT
    CAGACTCCTG GAAACCTTCT TCATGTCTTC TTCACAAATG TCCGTCCTCC AAAGAAGGTG
    CTGGAGGACC AGCTTACTCA GATTCTAAGG AAATACGGTG TGCCCAAGCC GAAATTTGAC
    AAGAGCAAAT ACAACAAGGC AGGCAAAGAA CAGCATCCGG TGAAGGTTGT GAGCACCAAG
    CGCCCAGTTA CAAAACCACC CACAAAAGAA AAGTCTGTAC TCAACAGCGT CAGCCGAACT
    GCTTTGAGTG AGAAGAAACC TACTGTAAAG CCAAAGTCTC CTGAGAAGAG CAAACCTGAA
    GAGAAGGACC CTGAAAAGTC TCCCACAAAG AAACAAGAAG TTCCTGAGGA GAAGTACCTG
    ACACTGGATG GATTTCACAG ATTTGTCATT GACCGATCTA AGCAGGATAT CCGTAGTGTG
    TGGAGAGCTA TTCTGTCCTG TGGATATGAT CTGCATTTTG AAAGATGTGC CTGCATTGAT
    GCAAGACATG CACAGAAAGC ATCTCGGAAG TGGACTCTGG AAATGGACGT GGCTCTCGTT
    CAGTACATTA ACAGGCTTTG TCGTCACCTC GCAATTACAC CAGCACGACT CCATCCACAT
    GAAGTATATT TGGATCCAGC TGATGCAGCA GATCCTAGAA TCTCCTGTCT GTTGAATGTT
    CCCGTTGAAA GTCTCCGCCT GCGGTTTGCA CTGCTTCAGT CTCTCAATAC GACGTTAGAG
    ACGTTTTTCT TACCGCTGGT AGAGCTTCGG CAGACTGAGA TGTACAGTAA CAGCATTGCT
    GCGCTGCTGC AGGAGGCCAA AGGTTTAATC TTCTATGACA CAAAAATAAC CGTAATGAAC
    AGAGTGCTGA ATGCCACTGT CCAAAGAACT GCTGATCACG CAGCCCCAGA AATCACCTTG
    GATCCCTTGG AAATAGTTGG AGGGGAGATC CGTTCTTCAG AAAATTCCTA CTTCTGCCAG
    GCAGCAAGGC AGCTGGCCTG TGTGCCATCC TCACAGCTCT GCGTCAAACT CGCCAGTGGT
    GGGGACCCTA CGTACGCGTT CAACATCCGG TTCACAGGGG AGGAGGTCCA TGGAACAAGT
    GGATCTTTCC GTCACTTCCT CTGGCAAGTT TGTAAGGAGC TGCAGAGCTC CTCGCTCTCT
    CTTCTCCTGT TGTGTCCAAG CTCTGCGGTC AACAAGAACA AGGGGAAGTA CATTTTGACA
    CCTAGCCCCA TAACCTATGC AGAGGAGCAG CTGTTCCATT TCTTTGGGCA GCTTCTGGGC
    ATTGCAATTC GAGCAGATGT TCCCCTGCCA CTCGACCTCC TGCCCTCATT CTGGAAGACT
    CTGGTAGGAG AGCCGCTGGA TCCTGATGTT GATCTCCAGG AAGCTGATAT CCTCACCTAC
    AACTACATTA AAAAGTTTGA AAATATAAAT GATGAAACTG AACTGGAAGC TCTGTGTGCA
    GAAATTGCTT CTCAGCATTT AGCAACAGAG AGCCCTGACT GTCCTAATAA GCCATGCTGC
    AAATTCACCT ACCTGACCAT GACAGGGGAA GAGGTGGAAC TCTGTCCCAG GGGCAGGCAC
    GTCCCAGTGG GATGGGAGAA CAAGGACGTG TATGCAGCTG CAATCAGGAG CTTGAGAATG
    CGTGAGCTGC AGACTCCAGA GTGCATGACT GCAGTGCGTG CTGGGCTTGG GTCCATCATT
    CCCCTGCAGC TTCTGACCAC GCTGACCCCT CTTGAGATGG AGCTGAGGAC ATGTGGGCTT
    CCATACATCA ACCTGGAGTT TCTCAAGGCG CACACGATGT ACCAGGTGGG ACTGATGGAA
    ACTGATCAGC ACATTGAGTT TTTTTGGAGC GCGCTGGAGA TGTTCACTCA GGAGGAGCTC
    TGCAAGTTCA TAAAGTTTGC CTGTAACCAG GAGCGGATCC CCTTCACGTG TCCTTGCAAG
    GACGGCGGTC CCGATACCGC CCACGTCCCG CCTTACCCCA TGAAAATAGC CCCCCCTGAT
    GGAGCTGCAG GTTCTCCGGA CTCTCGGTAC ATCCGTGTGG AAACGTGCAT GTTCATGATC
    AAACTTCCTC AGTACTCATC ACTGGACATC ATGCTGGAGA AGCTCCGATA CGCCATCCAC
    TACCGGGAGG ATCCTCTCAG CGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42924
    Clone ID Related Accession (Same CDS sequence) XM_015275540.1
    Accession Version XM_015275540.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
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    1501
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGAGCT GAAGACCAGC
    GTTTTGTCTC TTGCTACACA AATTCTGACA GGATGTGATG AAGTCTTAGA AATGCTGCAG
    CAAGTCACTA CAGCACTAAT AAACAGTGAT ATTGCTGACA GAGAACAAAG ATTAAAAGGC
    CTGGAACAAA TTACGAAAGC TACTATGCTT GGTCATCTGC TTCCTGTCCT TCTGACATCC
    CTGATGCATC CAAATTTGCA AACTCTAACC ATGGCTGATG CCTTGATGCC TCAGCTGGTG
    CAGCTGGTTC TCTATACAAG TCAGACTGCA CTGCTGCTTA AAACTCAGTC TCCGGTTTTC
    TCAGAACTGA ACAGCTCTCC TGGTGGTGTG TCGGAACACA AAGGGAAATT GTTACATGAT
    GAGAGAATGC TGGAGGAGAA GGAAGAGCCA GGTTTTCTTA CAGGCTTGAA GATCCCTGCT
    CCTTGGGCTG CTGGGAAGAC TGTAGAAACT GTTCATCCTG TCAGGGATAA CTATAAATTT
    AAGGAAACTG TACACATACC AGGAGCCCGC TGTTTGTATC TTAGATTTGA CAACAGATGT
    TCTTCACAAT ATGATTACGA TAAGTCTAAC AGTGTATTTT ATGTTTTGAA GTTGGTGATC
    TATGCTGGTC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTAGTAAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGG AGTGGTCGTG AGCACAACAC TCCAGACAAA
    GCCATGTGGG GCTTTGCTTG CACTGTTCGA GCACAGGAGT CTTCAGAGGA TGTGTCAGGA
    GGCCTGCCAT TCTTAGTTGA CCTGGCACTT GGCCTTTCTG TGTTAGCTTG TTCTATGCTT
    CGGATTTTAT ACAATGGACC AGAAATTACC AAAGATGAAG AAACCTGTCA AGAGCTTCTA
    AGATCTAAAC TATTACAAAG GTGCCAATGG CAGGTGGAAG CTAATGGTGT AATATCTCCA
    GCCCTTACAC CGAGTCCTTC TCCACTGCCT CTTACTATAG ATGAGGACAG AGAATTTACT
    TATCCTTCTG ATGTGCTGGT GCCTCCCATT GGACACTATT TTGATCTGCC TAGGATTAGG
    CTGCCTCCAG GAATCATGAT AAAACTCAGG GAAATTTCTG GACGTGCTCG GCCTCAATTT
    AGACCTAGCA TAAAGGAAGT GATTCAGCCC GAGGTAATGG AGGAGATGGT AGTTTCATGT
    GTGATTAAAC ATTTGAATTT GGTTGATGCA CTCCAGTCCC TGATAAACTT CCAGTATCAG
    GAAGAACATG CTGAAGAATA TGATTTACTT TGTAAAATTA TGGGAGAGAC TTTTAAGAAG
    CTAAATGCCA TGGAGAGACA ATTACAGAGT GTGGCTGAGC TGGAACAGAA ATGGCAGAGT
    GAGGTTGATG ACGCCATGCA TGGGAAGCTG GAGAATAATG TTCCCTTCTT CTATGATTAC
    CACTTCAATG AGAGCAAGAT GAAAGAACTG GAACTTCTGT GTTCTATGAA GGAAGTTTCT
    TTTGATGGAA GTGATCTTGA GAATGTGGTC CTGGCATTAA GGGAGAAGTT CTTTCAAGAG
    GTGAATTCAC TGATACAGAA GACTTATCAT CCCCTAGCGA AAACAAAAGC ATTAGTTAAA
    AGCTTGATGA ACAGGGCAGA GCTGTTGCTG CATGTCACTA TTGCAGCGCA GTCTGGTATC
    ACAAGAAGTA TATCTGGAAC CCCTGCAGAG ACTCCAGCCT GTAAGTCTGC ATCTGAAACA
    AAAGTGATAT CTCATGCAGT GCGCCAGCCC GTCTTCCTTC GCAGTATGTC AGCACCATCT
    GACTTAGAGA TGATTGGTAA CGAAGATATT GAGTTTGCTC GATCAAGTCA AAGGCGACGC
    CATGTTACCA GCCACAGAAG TAGCTCTTTC ACTCTTCTGC AATCACTGAC CATTGAGGAC
    AGCAGAGATA AACCTACATA CAGTGTCCTA CTGGGGCAGC TCTTTGCTTT CATTGGAACT
    AATCCTGACC AAGCGGTATC CAGCAGCAGT TTTCTGCTCG CTGCTCAGAC AAGGTGGCGG
    CGTGGGAACA CAAGGAAGCA AGCCTTAGTG CATATGAGAG AGCTGCTGAC TGCGGCTGTT
    CGGGTTGGTG GGGTGACACA CCTCGTGGGC CCTGTGACCA TGGTACTTCA GGGAGGACCA
    AGAATTGAAG AGCTCACGTG TGGTGGGATG GTGGAACAAG TCCAGGAAGC TTTTGGGGAG
    ACTATGACAT CCGTGGTCTC GCTGTGTGCT CGCTACCCCA TTGCTTGTGC AAACAGCATT
    GGCCTCTTAT GCACTATACC TTACACAAGG AGTGAAGAGA AGTGTTTGGT ACGTAGTGGC
    CTGGTACAGC TTATGGACAG GCTGTGCAGC TTAAGCAGCC AGGCAGAATC CAGTTCAAAT
    GAAAAACAGA CCAAGAAGCA GAAGGTGGCC ACCATGGCTT GGGCTGCCTT TCAGGTGCTG
    GCCAATCGCT GTGTTGAATG GGAGAAAGAA GAAGGTGGTT CAACAGAAGC TGTTCACTCT
    GGTCTGGCTC GTCAGGTCTC CAGCCTTCTT ACCAATCACC TTGCACGAGC TACAGAGTGT
    TGTGGTAATC AAGCTGCAGG AAATGATGCA CTTCAAGACG TCCTCAGTCT TCTTAATGAC
    CTGTCGAGGA GTCACATAGG TAAAGCAATC CTGAGCCAGC CAGCCTGTGT GTCCAAACTT
    CTGTCACTTC TCTTGGATCA ACGTCCCTCT CCAAAGTTGG TTCTTATAAT CCTTCAGTTG
    TGTCGTGCTG CTCTACCTCT CATGAGTGTT GAAGACTGTG GAAATGTAGA ACTGCCTCCC
    TGGAGTTACT CTGTGCCCTC CCTGAACAGT GAACAAGATG ATCCCAGCGA CCCAGCCTCC
    AAGATTGCAT CTCTGCTCTT AGCAAAGCTG GCAGATTATG TAGTACCAGG ATGCCAGACA
    GTTCTTTCTC CAACTGCTTC TGAACCAGAT ACTGCTTTAG CAAAAAGCAG CCCTAAGAAT
    TCTATAAAAG GCGATAAAGA CCCTGGAGAA GAAAGTGAGG CAGTGGATGG CAAACTTTCT
    ATTTTCATCC ATAAACGGGA AGACCAGTCA TCCCACGAAG TGCTTCAGCC ATTGCTGAGT
    AGCTCCGAAG GTCGCCCATT CCGACTAGGA ACTGGAGCCA ACATGGAGAA GGTGGTGAAA
    ATGGATCGTG ACATGACTAA AAGTGGCTGC TGTGAAGTTA TCACAGAAGA AGCTGCTGCA
    GCTCTTCGCA AGGCAACTAA GTGGGCTCAG TCTGGTCTGA TAGTTAGTGT TGGGCCACCC
    ATAGAAACTG TCAATCCAGA AGCAACCAGT GGGATGTCCT CTGGGGACAA AAAGAAGACT
    GCACAGACAT CTATTTGCAG AGAGAGAAAT TCTGAGCTTG CCAGGACTGA TCCTGTAAGA
    CCATTCATCA GTGGTCATGT TGCAAATAGC ATGGCAGCAG AAGTGATCGC TTTGCTACAT
    AGCTTGTTGA TGGCACCAGA ATCAAATGCG GCTCAAATAT GGACAACAAC TGCTGAAAAA
    GTTCTGTCTC GGGCTCTGAT GTACATTCCA CAACTCGGGA AATACGCAGA GAGTATTTTG
    GAGAATGGAA GCAGCAGTGG AAGGAAGCTT GCTAAACTTC AAAGAATTGC TCGACAGGCA
    GTAGCTGCTC TTTGTGCTCT AGGTGGCTTT AAGGAGACAA TTAAAATCGG TTCTGAAGTT
    CAGGTTTTAG GTAAAGGAAT AGCAGGAAGC ATTGGAGTTG TGGCATCTAT TAATGAACAA
    GAAGGTATAG CAACGGTCAA ATTTCCGCCT ACCACTATAG ACAGTAGAAA GACCTCACAA
    GCATCAGACA CTTTAACTAT TCCGTTATCT AGGCTCTGTG TTCCAAGATC TGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTATC TATTCACACC TCACTTCCTG CAACAGGAGA TGGCTCAACT
    CCAGTAATGG CAGTGGTCCG GCTCCTTGCT GAAATAAGAA CCAGAGCATG CCTGGTGATG
    GCTCAGCTGT TAGAAGACAG TTCATTCTGT GAAGAATTCA TTCAACAGTG CCCAGCTGCT
    GTAGAGGTTC TTAATTTGGT AGCCCAGGAG TGCAGCCCTG GAGAGAGGCT CACTGTAGTA
    GAAATGCAGT GTGAAAGGTT AAGGATGTTG TATCGTGACT GTGCTCGACC CCCACCTCCT
    CCACTGCAGG CAGACAGAAG GCAGCCCAAA GAAATCACTT GGAGCCCATC AAGAGTGTTT
    CCCCCAGTAC GAGCATGCAT GTTTTCATCA CATCTTGCTG CTGTGACCTT CCTGGCTGAT
    CCAAGTGCAG GTGGGGGACT TCCTCGGGGA ACGTTCATTT ATGCCACTTC TCCAGTTCCC
    GTGCAGGCAC CATCATTCTA CTGGGAAATT GAAATAGTTT CCTATGGAGA TACAGATGAT
    GACACTGGTC CAATAGTTTC ATTTGGATTC GCTACAGAGG CAGAAAAACG GGATGGGGCT
    TGGACAAACC CAGTGGGCAC CTGTCTTTTT CATAACAATG GGCGAGCAGT GCATTACAAT
    GGCTCCAGCT TGTTACAGTG GAAGAGTGTT CGATTGGATG TGACTCTTTC TCCTGGTGAT
    GTGGCTGGAA TTGGATGGGA AAGAGCAGAA GGAACTCCAC CCCCACCAGG GCAGCCTGCT
    AAAGGCAGAG TTTATTTTAC TTATTGCGGG CAGCGACTTG GGCCATACCT AGAGGATGTC
    TCTGGTGGAA TGTGGCCAAT TGTTCACATA CAGAAAAAGA ACACAAAAGT CCGTGCTAAT
    TTTGGATCTC GCCAGTTTGC CTATGCCGAA GGCCAAGCTC ACAGGAATGC TGCTGATCTG
    TGTATTGACC TAGCTGAAGA GATAAGTGCC AACTTTGAGG CATTGCCATT TGCTATGGCT
    TCTGATAGCG ATAACGACGC TGGCACCAGC ATAGCTTCTG ATCCTGGGAC TCACGGACCT
    CCCTGTAGAA TTGCTGCTGT TGCTACGGCT CAACAACAAT ATGACAGTGA CACATCTTGT
    CACTATAAAA TGGAACTAAG CTATGAGAAC TTCATCACAT CAGGGCCTGA TCCTCATCCC
    CCTCCTATTG CAGATGATGA AAGTGATGAT GACGACGATG ATGATATTCC CAGGGAGGAC
    CACTATGCCC TGCTGGTTAA AGCCTGGGAA ACTAAAGTTT TCCCTACAAT TAGAAGAAGA
    TTCCGTAATG AGGCTGAGAG GAAATCTGGG CTGGATCAAA TTAAAGGAGC CTTACAGTTA
    GGAATGGTTG ATATAGCACG ACAAACTGTA GAATTCTTGT ATGAGGAAAA CGGTGGCATC
    CCGAGAGACC TCTACCTTCC TACCATTGAG GATATCAAAG ATGAAGCAAA CAAGTTTACA
    ATCGATAAAG TACGAAAAGG TCTCACTGTT GTAACTCGCT CTCCTGACAG TAACAACGTT
    ACCAGCAGTG CTGTTGGAAC AGCTTTACCC AAGTTTGCTA TTCGGGGAAT GCTGAAGACA
    TTTGGTCTTC ATGGTGTTGT CCTGGATGTT GATTCAGTGA ATGAATTGGT ACAAGTAGAG
    ACCTATCTCA GAAGTGAGGG AGTTCTGGTA AGATATTGGT ATCCTATTGA CATGTTAGAA
    AGGCCACCTG CTGGTTATAG GAGGACTGCA ACAAATGGAT TGGTTACCCT GGACAATACC
    AATATCCAAA TCCACAGAGA GCTTTTGAGA AGTGAAGCTG CACTGGCCAA GCTGTACTGC
    CGCATGGCTC TCCTCAATAT CTTTGCCCCC AAATCTCCAC ATATGTTCAC CCGTCTGTTT
    CATATCCCCG CTATCCGTGA TATCACTCTG GAGCACCTGC AGTTGCTATC CAACCAACTT
    CTCGCACCAC CTCTTCCTGA TGGAACAATC AGTTCAAGTT CCATTCTTTT GGCTCAGTCA
    CTGCAACACT GCATCCATTC CCAGAACTGT GCTGCCACAG ACCTCTTCTA CCAGGGCAAT
    TCACAAGCCA TCAGAGAGTG GCTTACTGTT GCCATCACTC GGACACTGCA TCAAGGAGAG
    GAGAGCCTGT TAGACCTCAC TAAGCAGATC TGCTCTTTTT TGCAGACTGC ACCAGAACAG
    TTTCCTGCTG AAGAATTCCC AATTTCAGAA TCCAAAGTCA ACATGGATGT TAATTTTCCT
    GGGGCTGCGT TTGTAATTGT GTCCTGTAAA GAAAGCCAGT CTGGATTCCG CAAAGATTCT
    TCCCTGTATA AAGCTCCTTG GGCTCGGGTG CTTGTGTACG GGCTGGGGCA CAAAGTTAAG
    AGGAATGGCC AGTTAAACCT GATTGAGGCA GTGTGTTACC CTCGAGACGC CTCTCCGACC
    AACACAGGCC TAACACCTCC CCCAACAACC AATCAGTACC CTTCAGTGAT CACTCCTACA
    GACAAAGTGC ACATCAAACT AGGAGTGTCT CCTCCTCCTG GAGCTGTGCT GGTCTTGCAT
    TCTCTTCCCC TTGAGTTCCC CCTGGCAATG GCATTTACAG AACAGCTGTT AACTTGGAAA
    CTGGAAGATG TGGATGGGAA ATCAGAAGAT GAACTGGATA CTATTCCTCC TTCAGTTCTT
    TTGCAAGTAG TGGAATTTTT AGGGAACTTT CTGTGGACAA CTGACATGGC AGCATCTGTA
    AAGGAATTGA TATTTCATCT CTTGGCTGAA TTGCTTCGCA AAATTCACAA CCTGGAGCAG
    AAGAAAAGTC CTGCAGGATT ATCATCTTCT ATTGCCCTTC AGCTGAATCC CTGTCTAGCT
    ATGCTTATGG CCCTGCAGTC AGAACTCCAT AAGCTGTACG ATGAGGAAAC ACAAAACTGG
    GTGTCTGGAA ATGCATGCGG TGGTTCTGGT GTTGGAGCTG CTGACCAAGG AAGATTTTCA
    ACATACTTCC ATGCTCTGAT GGAAGTTTGC CTTGCTGTAG CAGAAGTAAC TCTACCTATC
    AATATGAGCG TAACAGCTAA TGTGATATCT TCAACGAGTG CACCAAATCT TAGTGACTCT
    TCCTCCTCAT CTTCATCTTC TCCAGGACAG ACACCGCAGA GTCCAAGCTT ATTGTCAAAG
    AGGAAAAAAG TCAAAATGAA GAGAGAAAAG ACATCGTCTT CAGGCAAACG ATCTTCATCC
    AGAGCAGCTG AAACGGATGC TGCAATCCTC AGTATAGGAG GAAGCAAGCC TGAGGATATG
    CTATGGTTTC ATAGAGCACT GACTTTGCTT ATCATTCTCA GACACCTAAC GAAAAAGGAT
    CCGCAAGGGT TGGGTGTGAC AAACGATGCT GTAGCAGATG CATGTCAGGC ACTGGTAGGA
    CCCACAGCTC ATAGCCGTTT GCTTGTCATT TCTGGAATAC CAACTCACTT GGAGGAGAGT
    ACTGTCAGAA ATGCAATCAG GAAGGCGTGT AATGCGCATG GTGGAGTCTT CAAGGATGAA
    ATCTACATCC CTCTGCAGGA AGAGGATCCC AAAAAAATCA AGGACAAAGC AGAAGGAGGA
    GAATGCAGAA CTGAGCTGGA AAAGCCAACT GTTTCAAGTA GTGCGGACAG TCTGGATATC
    AGCAGCTCTA GCAGTGTCAC ACCTGCTATG AGCGTTAGTG CGTCAGCATC AACGAGCCAG
    GCTTCCCTGT GCAGCTCACA GGGTATCTCC CGGACTGTTA GTGACATCTC TGTTGATCAG
    TACCAGGCTG GGCTGGAACT GGCCATCCCA CCAGGACTGC TGGAGCCACA CGTTTCCAGC
    CAGGAGAGCT TGGATCTGTC GCACTGCAGT ACTGGGAGCC TTGGCAGCCT TGGCAGCCTC
    GGGGAGCCGC TTGACAATGT AGAGACAGCA TCAGTGTCTG ATGTCGGGTC GATGTACACG
    GTCACATCTC TGGACAACCA GCAACTTCTG TCACGGCCTA TCAAAGGTTT TGCTGTGGTG
    GAGATACGAT CAAGAGCTAA AATAGAGAAA ATCCGAGCCA GCCTATTTAA CAGTAGTGAT
    CTCATTGGTT TGTCAAGTCT GGATGGTGAA GACGAACTGA TGGAAATGTC AACTGAAGAG
    ATTTTAACTG TTTCTACTGT AAATCAGAGT TTATTTGACA CACAAGGGAG CCCGGCGCTA
    GAAGACTACT TCAATGATAA GTCAATAAAA GGTGAGAAAT TGGTTCCTGG TGCTCGGGAG
    GTCCTAACAG AAATATTTAA AAGCTGTGTC CATTCTGAGC AAATGCTGAG CCTGACTCCA
    ACAAAACCAA TCAAGGTTGC TGATATCTAT CTTAGTAAAG AGCAGATAAA TTCTCAGACT
    CCTGGAAACC TTCTTCATGT CTTCTTCACA AATGTCCGTC CTCCAAAGAA GGTGCTGGAG
    GACCAGCTTA CTCAGATTCT AAGGAAATAC GGTGTGCCCA AGCCGAAATT TGACAAGAGC
    AAATACAACA AGGCAGGCAA AGAACAGCAT CCGGTGAAGG TTGTGAGCAC CAAGCGCCCA
    GTTACAAAAC CACCCACAAA AGAAAAGTCT GTACTCAACA GCGTCAGCCG AACTGCTTTG
    AGTGAGAAGA AACCTACTGT AAAGCCAAAG TCTCCTGAGA AGAGCAAACC TGAAGAGAAG
    GACCCTGAAA AGTCTCCCAC AAAGAAACAA GAAGTTCCTG AGGAGAAGTA CCTGACACTG
    GATGGATTTC ACAGATTTGT CATTGACCGA TCTAAGCAGG ATATCCGTAG TGTGTGGAGA
    GCTATTCTGT CCTGTGGATA TGATCTGCAT TTTGAAAGAT GTGCCTGCAT TGATGCAAGA
    CATGCACAGA AAGCATCTCG GAAGTGGACT CTGGAAATGG ACGTGGCTCT CGTTCAGTAC
    ATTAACAGGC TTTGTCGTCA CCTCGCAATT ACACCAGCAC GACTCCATCC ACATGAAGTA
    TATTTGGATC CAGCTGATGC AGCAGATCCT AGAATCTCCT GTCTGTTGAA TGTTCCCGTT
    GAAAGTCTCC GCCTGCGGTT TGCACTGCTT CAGTCTCTCA ATACGACGTT AGAGACGTTT
    TTCTTACCGC TGGTAGAGCT TCGGCAGACT GAGATGTACA GTAACAGCAT TGCTGCGCTG
    CTGCAGGAGG CCAAAGGTTT AATCTTCTAT GACACAAAAA TAACCGTAAT GAACAGAGTG
    CTGAATGCCA CTGTCCAAAG AACTGCTGAT CACGCAGCCC CAGAAATCAC CTTGGATCCC
    TTGGAAATAG TTGGAGGGGA GATCCGTTCT TCAGAAAATT CCTACTTCTG CCAGGCAGCA
    AGGCAGCTGG CCTGTGTGCC ATCCTCACAG CTCTGCGTCA AACTCGCCAG TGGTGGGGAC
    CCTACGTACG CGTTCAACAT CCGGTTCACA GGGGAGGAGG TCCATGGAAC AAGTGGATCT
    TTCCGTCACT TCCTCTGGCA AGTTTGTAAG GAGCTGCAGA GCTCCTCGCT CTCTCTTCTC
    CTGTTGTGTC CAAGCTCTGC GGTCAACAAG AACAAGGGGA AGTACATTTT GACACCTAGC
    CCCATAACCT ATGCAGAGGA GCAGCTGTTC CATTTCTTTG GGCAGCTTCT GGGCATTGCA
    ATTCGAGCAG ATGTTCCCCT GCCACTCGAC CTCCTGCCCT CATTCTGGAA GACTCTGGTA
    GGAGAGCCGC TGGATCCTGA TGTTGATCTC CAGGAAGCTG ATATCCTCAC CTACAACTAC
    ATTAAAAAGT TTGAAAATAT AAATGATGAA ACTGAACTGG AAGCTCTGTG TGCAGAAATT
    GCTTCTCAGC ATTTAGCAAC AGAGAGCCCT GACTGTCCTA ATAAGCCATG CTGCAAATTC
    ACCTACCTGA CCATGACAGG GGAAGAGGTG GAACTCTGTC CCAGGGGCAG GCACGTCCCA
    GTGGGATGGG AGAACAAGGA CGTGTATGCA GCTGCAATCA GGAGCTTGAG AATGCGTGAG
    CTGCAGACTC CAGAGTGCAT GACTGCAGTG CGTGCTGGGC TTGGGTCCAT CATTCCCCTG
    CAGCTTCTGA CCACGCTGAC CCCTCTTGAG ATGGAGCTGA GGACATGTGG GCTTCCATAC
    ATCAACCTGG AGTTTCTCAA GGCGCACACG ATGTACCAGG TGGGACTGAT GGAAACTGAT
    CAGCACATTG AGTTTTTTTG GAGCGCGCTG GAGATGTTCA CTCAGGAGGA GCTCTGCAAG
    TTCATAAAGT TTGCCTGTAA CCAGGAGCGG ATCCCCTTCA CGTGTCCTTG CAAGGACGGC
    GGTCCCGATA CCGCCCACGT CCCGCCTTAC CCCATGAAAA TAGCCCCCCC TGATGGAGCT
    GCAGGTTCTC CGGACTCTCG GTACATCCGT GTGGAAACGT GCATGTTCAT GATCAAACTT
    CCTCAGTACT CATCACTGGA CATCATGCTG GAGAAGCTCC GATACGCCAT CCACTACCGG
    GAGGATCCTC TCAGCGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131026.1
    CDS263..13543
    Translation

    Target ORF information:

    RefSeq Version XM_015275540.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGAGCT GAAGACCAGC
    GTTTTGTCTC TTGCTACACA AATTCTGACA GGATGTGATG AAGTCTTAGA AATGCTGCAG
    CAAGTCACTA CAGCACTAAT AAACAGTGAT ATTGCTGACA GAGAACAAAG ATTAAAAGGC
    CTGGAACAAA TTACGAAAGC TACTATGCTT GGTCATCTGC TTCCTGTCCT TCTGACATCC
    CTGATGCATC CAAATTTGCA AACTCTAACC ATGGCTGATG CCTTGATGCC TCAGCTGGTG
    CAGCTGGTTC TCTATACAAG TCAGACTGCA CTGCTGCTTA AAACTCAGTC TCCGGTTTTC
    TCAGAACTGA ACAGCTCTCC TGGTGGTGTG TCGGAACACA AAGGGAAATT GTTACATGAT
    GAGAGAATGC TGGAGGAGAA GGAAGAGCCA GGTTTTCTTA CAGGCTTGAA GATCCCTGCT
    CCTTGGGCTG CTGGGAAGAC TGTAGAAACT GTTCATCCTG TCAGGGATAA CTATAAATTT
    AAGGAAACTG TACACATACC AGGAGCCCGC TGTTTGTATC TTAGATTTGA CAACAGATGT
    TCTTCACAAT ATGATTACGA TAAGTCTAAC AGTGTATTTT ATGTTTTGAA GTTGGTGATC
    TATGCTGGTC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTAGTAAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGG AGTGGTCGTG AGCACAACAC TCCAGACAAA
    GCCATGTGGG GCTTTGCTTG CACTGTTCGA GCACAGGAGT CTTCAGAGGA TGTGTCAGGA
    GGCCTGCCAT TCTTAGTTGA CCTGGCACTT GGCCTTTCTG TGTTAGCTTG TTCTATGCTT
    CGGATTTTAT ACAATGGACC AGAAATTACC AAAGATGAAG AAACCTGTCA AGAGCTTCTA
    AGATCTAAAC TATTACAAAG GTGCCAATGG CAGGTGGAAG CTAATGGTGT AATATCTCCA
    GCCCTTACAC CGAGTCCTTC TCCACTGCCT CTTACTATAG ATGAGGACAG AGAATTTACT
    TATCCTTCTG ATGTGCTGGT GCCTCCCATT GGACACTATT TTGATCTGCC TAGGATTAGG
    CTGCCTCCAG GAATCATGAT AAAACTCAGG GAAATTTCTG GACGTGCTCG GCCTCAATTT
    AGACCTAGCA TAAAGGAAGT GATTCAGCCC GAGGTAATGG AGGAGATGGT AGTTTCATGT
    GTGATTAAAC ATTTGAATTT GGTTGATGCA CTCCAGTCCC TGATAAACTT CCAGTATCAG
    GAAGAACATG CTGAAGAATA TGATTTACTT TGTAAAATTA TGGGAGAGAC TTTTAAGAAG
    CTAAATGCCA TGGAGAGACA ATTACAGAGT GTGGCTGAGC TGGAACAGAA ATGGCAGAGT
    GAGGTTGATG ACGCCATGCA TGGGAAGCTG GAGAATAATG TTCCCTTCTT CTATGATTAC
    CACTTCAATG AGAGCAAGAT GAAAGAACTG GAACTTCTGT GTTCTATGAA GGAAGTTTCT
    TTTGATGGAA GTGATCTTGA GAATGTGGTC CTGGCATTAA GGGAGAAGTT CTTTCAAGAG
    GTGAATTCAC TGATACAGAA GACTTATCAT CCCCTAGCGA AAACAAAAGC ATTAGTTAAA
    AGCTTGATGA ACAGGGCAGA GCTGTTGCTG CATGTCACTA TTGCAGCGCA GTCTGGTATC
    ACAAGAAGTA TATCTGGAAC CCCTGCAGAG ACTCCAGCCT GTAAGTCTGC ATCTGAAACA
    AAAGTGATAT CTCATGCAGT GCGCCAGCCC GTCTTCCTTC GCAGTATGTC AGCACCATCT
    GACTTAGAGA TGATTGGTAA CGAAGATATT GAGTTTGCTC GATCAAGTCA AAGGCGACGC
    CATGTTACCA GCCACAGAAG TAGCTCTTTC ACTCTTCTGC AATCACTGAC CATTGAGGAC
    AGCAGAGATA AACCTACATA CAGTGTCCTA CTGGGGCAGC TCTTTGCTTT CATTGGAACT
    AATCCTGACC AAGCGGTATC CAGCAGCAGT TTTCTGCTCG CTGCTCAGAC AAGGTGGCGG
    CGTGGGAACA CAAGGAAGCA AGCCTTAGTG CATATGAGAG AGCTGCTGAC TGCGGCTGTT
    CGGGTTGGTG GGGTGACACA CCTCGTGGGC CCTGTGACCA TGGTACTTCA GGGAGGACCA
    AGAATTGAAG AGCTCACGTG TGGTGGGATG GTGGAACAAG TCCAGGAAGC TTTTGGGGAG
    ACTATGACAT CCGTGGTCTC GCTGTGTGCT CGCTACCCCA TTGCTTGTGC AAACAGCATT
    GGCCTCTTAT GCACTATACC TTACACAAGG AGTGAAGAGA AGTGTTTGGT ACGTAGTGGC
    CTGGTACAGC TTATGGACAG GCTGTGCAGC TTAAGCAGCC AGGCAGAATC CAGTTCAAAT
    GAAAAACAGA CCAAGAAGCA GAAGGTGGCC ACCATGGCTT GGGCTGCCTT TCAGGTGCTG
    GCCAATCGCT GTGTTGAATG GGAGAAAGAA GAAGGTGGTT CAACAGAAGC TGTTCACTCT
    GGTCTGGCTC GTCAGGTCTC CAGCCTTCTT ACCAATCACC TTGCACGAGC TACAGAGTGT
    TGTGGTAATC AAGCTGCAGG AAATGATGCA CTTCAAGACG TCCTCAGTCT TCTTAATGAC
    CTGTCGAGGA GTCACATAGG TAAAGCAATC CTGAGCCAGC CAGCCTGTGT GTCCAAACTT
    CTGTCACTTC TCTTGGATCA ACGTCCCTCT CCAAAGTTGG TTCTTATAAT CCTTCAGTTG
    TGTCGTGCTG CTCTACCTCT CATGAGTGTT GAAGACTGTG GAAATGTAGA ACTGCCTCCC
    TGGAGTTACT CTGTGCCCTC CCTGAACAGT GAACAAGATG ATCCCAGCGA CCCAGCCTCC
    AAGATTGCAT CTCTGCTCTT AGCAAAGCTG GCAGATTATG TAGTACCAGG ATGCCAGACA
    GTTCTTTCTC CAACTGCTTC TGAACCAGAT ACTGCTTTAG CAAAAAGCAG CCCTAAGAAT
    TCTATAAAAG GCGATAAAGA CCCTGGAGAA GAAAGTGAGG CAGTGGATGG CAAACTTTCT
    ATTTTCATCC ATAAACGGGA AGACCAGTCA TCCCACGAAG TGCTTCAGCC ATTGCTGAGT
    AGCTCCGAAG GTCGCCCATT CCGACTAGGA ACTGGAGCCA ACATGGAGAA GGTGGTGAAA
    ATGGATCGTG ACATGACTAA AAGTGGCTGC TGTGAAGTTA TCACAGAAGA AGCTGCTGCA
    GCTCTTCGCA AGGCAACTAA GTGGGCTCAG TCTGGTCTGA TAGTTAGTGT TGGGCCACCC
    ATAGAAACTG TCAATCCAGA AGCAACCAGT GGGATGTCCT CTGGGGACAA AAAGAAGACT
    GCACAGACAT CTATTTGCAG AGAGAGAAAT TCTGAGCTTG CCAGGACTGA TCCTGTAAGA
    CCATTCATCA GTGGTCATGT TGCAAATAGC ATGGCAGCAG AAGTGATCGC TTTGCTACAT
    AGCTTGTTGA TGGCACCAGA ATCAAATGCG GCTCAAATAT GGACAACAAC TGCTGAAAAA
    GTTCTGTCTC GGGCTCTGAT GTACATTCCA CAACTCGGGA AATACGCAGA GAGTATTTTG
    GAGAATGGAA GCAGCAGTGG AAGGAAGCTT GCTAAACTTC AAAGAATTGC TCGACAGGCA
    GTAGCTGCTC TTTGTGCTCT AGGTGGCTTT AAGGAGACAA TTAAAATCGG TTCTGAAGTT
    CAGGTTTTAG GTAAAGGAAT AGCAGGAAGC ATTGGAGTTG TGGCATCTAT TAATGAACAA
    GAAGGTATAG CAACGGTCAA ATTTCCGCCT ACCACTATAG ACAGTAGAAA GACCTCACAA
    GCATCAGACA CTTTAACTAT TCCGTTATCT AGGCTCTGTG TTCCAAGATC TGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTATC TATTCACACC TCACTTCCTG CAACAGGAGA TGGCTCAACT
    CCAGTAATGG CAGTGGTCCG GCTCCTTGCT GAAATAAGAA CCAGAGCATG CCTGGTGATG
    GCTCAGCTGT TAGAAGACAG TTCATTCTGT GAAGAATTCA TTCAACAGTG CCCAGCTGCT
    GTAGAGGTTC TTAATTTGGT AGCCCAGGAG TGCAGCCCTG GAGAGAGGCT CACTGTAGTA
    GAAATGCAGT GTGAAAGGTT AAGGATGTTG TATCGTGACT GTGCTCGACC CCCACCTCCT
    CCACTGCAGG CAGACAGAAG GCAGCCCAAA GAAATCACTT GGAGCCCATC AAGAGTGTTT
    CCCCCAGTAC GAGCATGCAT GTTTTCATCA CATCTTGCTG CTGTGACCTT CCTGGCTGAT
    CCAAGTGCAG GTGGGGGACT TCCTCGGGGA ACGTTCATTT ATGCCACTTC TCCAGTTCCC
    GTGCAGGCAC CATCATTCTA CTGGGAAATT GAAATAGTTT CCTATGGAGA TACAGATGAT
    GACACTGGTC CAATAGTTTC ATTTGGATTC GCTACAGAGG CAGAAAAACG GGATGGGGCT
    TGGACAAACC CAGTGGGCAC CTGTCTTTTT CATAACAATG GGCGAGCAGT GCATTACAAT
    GGCTCCAGCT TGTTACAGTG GAAGAGTGTT CGATTGGATG TGACTCTTTC TCCTGGTGAT
    GTGGCTGGAA TTGGATGGGA AAGAGCAGAA GGAACTCCAC CCCCACCAGG GCAGCCTGCT
    AAAGGCAGAG TTTATTTTAC TTATTGCGGG CAGCGACTTG GGCCATACCT AGAGGATGTC
    TCTGGTGGAA TGTGGCCAAT TGTTCACATA CAGAAAAAGA ACACAAAAGT CCGTGCTAAT
    TTTGGATCTC GCCAGTTTGC CTATGCCGAA GGCCAAGCTC ACAGGAATGC TGCTGATCTG
    TGTATTGACC TAGCTGAAGA GATAAGTGCC AACTTTGAGG CATTGCCATT TGCTATGGCT
    TCTGATAGCG ATAACGACGC TGGCACCAGC ATAGCTTCTG ATCCTGGGAC TCACGGACCT
    CCCTGTAGAA TTGCTGCTGT TGCTACGGCT CAACAACAAT ATGACAGTGA CACATCTTGT
    CACTATAAAA TGGAACTAAG CTATGAGAAC TTCATCACAT CAGGGCCTGA TCCTCATCCC
    CCTCCTATTG CAGATGATGA AAGTGATGAT GACGACGATG ATGATATTCC CAGGGAGGAC
    CACTATGCCC TGCTGGTTAA AGCCTGGGAA ACTAAAGTTT TCCCTACAAT TAGAAGAAGA
    TTCCGTAATG AGGCTGAGAG GAAATCTGGG CTGGATCAAA TTAAAGGAGC CTTACAGTTA
    GGAATGGTTG ATATAGCACG ACAAACTGTA GAATTCTTGT ATGAGGAAAA CGGTGGCATC
    CCGAGAGACC TCTACCTTCC TACCATTGAG GATATCAAAG ATGAAGCAAA CAAGTTTACA
    ATCGATAAAG TACGAAAAGG TCTCACTGTT GTAACTCGCT CTCCTGACAG TAACAACGTT
    ACCAGCAGTG CTGTTGGAAC AGCTTTACCC AAGTTTGCTA TTCGGGGAAT GCTGAAGACA
    TTTGGTCTTC ATGGTGTTGT CCTGGATGTT GATTCAGTGA ATGAATTGGT ACAAGTAGAG
    ACCTATCTCA GAAGTGAGGG AGTTCTGGTA AGATATTGGT ATCCTATTGA CATGTTAGAA
    AGGCCACCTG CTGGTTATAG GAGGACTGCA ACAAATGGAT TGGTTACCCT GGACAATACC
    AATATCCAAA TCCACAGAGA GCTTTTGAGA AGTGAAGCTG CACTGGCCAA GCTGTACTGC
    CGCATGGCTC TCCTCAATAT CTTTGCCCCC AAATCTCCAC ATATGTTCAC CCGTCTGTTT
    CATATCCCCG CTATCCGTGA TATCACTCTG GAGCACCTGC AGTTGCTATC CAACCAACTT
    CTCGCACCAC CTCTTCCTGA TGGAACAATC AGTTCAAGTT CCATTCTTTT GGCTCAGTCA
    CTGCAACACT GCATCCATTC CCAGAACTGT GCTGCCACAG ACCTCTTCTA CCAGGGCAAT
    TCACAAGCCA TCAGAGAGTG GCTTACTGTT GCCATCACTC GGACACTGCA TCAAGGAGAG
    GAGAGCCTGT TAGACCTCAC TAAGCAGATC TGCTCTTTTT TGCAGACTGC ACCAGAACAG
    TTTCCTGCTG AAGAATTCCC AATTTCAGAA TCCAAAGTCA ACATGGATGT TAATTTTCCT
    GGGGCTGCGT TTGTAATTGT GTCCTGTAAA GAAAGCCAGT CTGGATTCCG CAAAGATTCT
    TCCCTGTATA AAGCTCCTTG GGCTCGGGTG CTTGTGTACG GGCTGGGGCA CAAAGTTAAG
    AGGAATGGCC AGTTAAACCT GATTGAGGCA GTGTGTTACC CTCGAGACGC CTCTCCGACC
    AACACAGGCC TAACACCTCC CCCAACAACC AATCAGTACC CTTCAGTGAT CACTCCTACA
    GACAAAGTGC ACATCAAACT AGGAGTGTCT CCTCCTCCTG GAGCTGTGCT GGTCTTGCAT
    TCTCTTCCCC TTGAGTTCCC CCTGGCAATG GCATTTACAG AACAGCTGTT AACTTGGAAA
    CTGGAAGATG TGGATGGGAA ATCAGAAGAT GAACTGGATA CTATTCCTCC TTCAGTTCTT
    TTGCAAGTAG TGGAATTTTT AGGGAACTTT CTGTGGACAA CTGACATGGC AGCATCTGTA
    AAGGAATTGA TATTTCATCT CTTGGCTGAA TTGCTTCGCA AAATTCACAA CCTGGAGCAG
    AAGAAAAGTC CTGCAGGATT ATCATCTTCT ATTGCCCTTC AGCTGAATCC CTGTCTAGCT
    ATGCTTATGG CCCTGCAGTC AGAACTCCAT AAGCTGTACG ATGAGGAAAC ACAAAACTGG
    GTGTCTGGAA ATGCATGCGG TGGTTCTGGT GTTGGAGCTG CTGACCAAGG AAGATTTTCA
    ACATACTTCC ATGCTCTGAT GGAAGTTTGC CTTGCTGTAG CAGAAGTAAC TCTACCTATC
    AATATGAGCG TAACAGCTAA TGTGATATCT TCAACGAGTG CACCAAATCT TAGTGACTCT
    TCCTCCTCAT CTTCATCTTC TCCAGGACAG ACACCGCAGA GTCCAAGCTT ATTGTCAAAG
    AGGAAAAAAG TCAAAATGAA GAGAGAAAAG ACATCGTCTT CAGGCAAACG ATCTTCATCC
    AGAGCAGCTG AAACGGATGC TGCAATCCTC AGTATAGGAG GAAGCAAGCC TGAGGATATG
    CTATGGTTTC ATAGAGCACT GACTTTGCTT ATCATTCTCA GACACCTAAC GAAAAAGGAT
    CCGCAAGGGT TGGGTGTGAC AAACGATGCT GTAGCAGATG CATGTCAGGC ACTGGTAGGA
    CCCACAGCTC ATAGCCGTTT GCTTGTCATT TCTGGAATAC CAACTCACTT GGAGGAGAGT
    ACTGTCAGAA ATGCAATCAG GAAGGCGTGT AATGCGCATG GTGGAGTCTT CAAGGATGAA
    ATCTACATCC CTCTGCAGGA AGAGGATCCC AAAAAAATCA AGGACAAAGC AGAAGGAGGA
    GAATGCAGAA CTGAGCTGGA AAAGCCAACT GTTTCAAGTA GTGCGGACAG TCTGGATATC
    AGCAGCTCTA GCAGTGTCAC ACCTGCTATG AGCGTTAGTG CGTCAGCATC AACGAGCCAG
    GCTTCCCTGT GCAGCTCACA GGGTATCTCC CGGACTGTTA GTGACATCTC TGTTGATCAG
    TACCAGGCTG GGCTGGAACT GGCCATCCCA CCAGGACTGC TGGAGCCACA CGTTTCCAGC
    CAGGAGAGCT TGGATCTGTC GCACTGCAGT ACTGGGAGCC TTGGCAGCCT TGGCAGCCTC
    GGGGAGCCGC TTGACAATGT AGAGACAGCA TCAGTGTCTG ATGTCGGGTC GATGTACACG
    GTCACATCTC TGGACAACCA GCAACTTCTG TCACGGCCTA TCAAAGGTTT TGCTGTGGTG
    GAGATACGAT CAAGAGCTAA AATAGAGAAA ATCCGAGCCA GCCTATTTAA CAGTAGTGAT
    CTCATTGGTT TGTCAAGTCT GGATGGTGAA GACGAACTGA TGGAAATGTC AACTGAAGAG
    ATTTTAACTG TTTCTACTGT AAATCAGAGT TTATTTGACA CACAAGGGAG CCCGGCGCTA
    GAAGACTACT TCAATGATAA GTCAATAAAA GGTGAGAAAT TGGTTCCTGG TGCTCGGGAG
    GTCCTAACAG AAATATTTAA AAGCTGTGTC CATTCTGAGC AAATGCTGAG CCTGACTCCA
    ACAAAACCAA TCAAGGTTGC TGATATCTAT CTTAGTAAAG AGCAGATAAA TTCTCAGACT
    CCTGGAAACC TTCTTCATGT CTTCTTCACA AATGTCCGTC CTCCAAAGAA GGTGCTGGAG
    GACCAGCTTA CTCAGATTCT AAGGAAATAC GGTGTGCCCA AGCCGAAATT TGACAAGAGC
    AAATACAACA AGGCAGGCAA AGAACAGCAT CCGGTGAAGG TTGTGAGCAC CAAGCGCCCA
    GTTACAAAAC CACCCACAAA AGAAAAGTCT GTACTCAACA GCGTCAGCCG AACTGCTTTG
    AGTGAGAAGA AACCTACTGT AAAGCCAAAG TCTCCTGAGA AGAGCAAACC TGAAGAGAAG
    GACCCTGAAA AGTCTCCCAC AAAGAAACAA GAAGTTCCTG AGGAGAAGTA CCTGACACTG
    GATGGATTTC ACAGATTTGT CATTGACCGA TCTAAGCAGG ATATCCGTAG TGTGTGGAGA
    GCTATTCTGT CCTGTGGATA TGATCTGCAT TTTGAAAGAT GTGCCTGCAT TGATGCAAGA
    CATGCACAGA AAGCATCTCG GAAGTGGACT CTGGAAATGG ACGTGGCTCT CGTTCAGTAC
    ATTAACAGGC TTTGTCGTCA CCTCGCAATT ACACCAGCAC GACTCCATCC ACATGAAGTA
    TATTTGGATC CAGCTGATGC AGCAGATCCT AGAATCTCCT GTCTGTTGAA TGTTCCCGTT
    GAAAGTCTCC GCCTGCGGTT TGCACTGCTT CAGTCTCTCA ATACGACGTT AGAGACGTTT
    TTCTTACCGC TGGTAGAGCT TCGGCAGACT GAGATGTACA GTAACAGCAT TGCTGCGCTG
    CTGCAGGAGG CCAAAGGTTT AATCTTCTAT GACACAAAAA TAACCGTAAT GAACAGAGTG
    CTGAATGCCA CTGTCCAAAG AACTGCTGAT CACGCAGCCC CAGAAATCAC CTTGGATCCC
    TTGGAAATAG TTGGAGGGGA GATCCGTTCT TCAGAAAATT CCTACTTCTG CCAGGCAGCA
    AGGCAGCTGG CCTGTGTGCC ATCCTCACAG CTCTGCGTCA AACTCGCCAG TGGTGGGGAC
    CCTACGTACG CGTTCAACAT CCGGTTCACA GGGGAGGAGG TCCATGGAAC AAGTGGATCT
    TTCCGTCACT TCCTCTGGCA AGTTTGTAAG GAGCTGCAGA GCTCCTCGCT CTCTCTTCTC
    CTGTTGTGTC CAAGCTCTGC GGTCAACAAG AACAAGGGGA AGTACATTTT GACACCTAGC
    CCCATAACCT ATGCAGAGGA GCAGCTGTTC CATTTCTTTG GGCAGCTTCT GGGCATTGCA
    ATTCGAGCAG ATGTTCCCCT GCCACTCGAC CTCCTGCCCT CATTCTGGAA GACTCTGGTA
    GGAGAGCCGC TGGATCCTGA TGTTGATCTC CAGGAAGCTG ATATCCTCAC CTACAACTAC
    ATTAAAAAGT TTGAAAATAT AAATGATGAA ACTGAACTGG AAGCTCTGTG TGCAGAAATT
    GCTTCTCAGC ATTTAGCAAC AGAGAGCCCT GACTGTCCTA ATAAGCCATG CTGCAAATTC
    ACCTACCTGA CCATGACAGG GGAAGAGGTG GAACTCTGTC CCAGGGGCAG GCACGTCCCA
    GTGGGATGGG AGAACAAGGA CGTGTATGCA GCTGCAATCA GGAGCTTGAG AATGCGTGAG
    CTGCAGACTC CAGAGTGCAT GACTGCAGTG CGTGCTGGGC TTGGGTCCAT CATTCCCCTG
    CAGCTTCTGA CCACGCTGAC CCCTCTTGAG ATGGAGCTGA GGACATGTGG GCTTCCATAC
    ATCAACCTGG AGTTTCTCAA GGCGCACACG ATGTACCAGG TGGGACTGAT GGAAACTGAT
    CAGCACATTG AGTTTTTTTG GAGCGCGCTG GAGATGTTCA CTCAGGAGGA GCTCTGCAAG
    TTCATAAAGT TTGCCTGTAA CCAGGAGCGG ATCCCCTTCA CGTGTCCTTG CAAGGACGGC
    GGTCCCGATA CCGCCCACGT CCCGCCTTAC CCCATGAAAA TAGCCCCCCC TGATGGAGCT
    GCAGGTTCTC CGGACTCTCG GTACATCCGT GTGGAAACGT GCATGTTCAT GATCAAACTT
    CCTCAGTACT CATCACTGGA CATCATGCTG GAGAAGCTCC GATACGCCAT CCACTACCGG
    GAGGATCCTC TCAGCGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42925
    Clone ID Related Accession (Same CDS sequence) XM_015275541.1
    Accession Version XM_015275541.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
    1381
    1441
    1501
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    12181
    12241
    12301
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    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGACTT AGGACGTTGT CTTGCAGCAG ATGGCCTCTA CCTTTATACA
    ACAAATTCAG TGGGAAGAGG AATAAGCAAA TTAGGATCTG GATTACATGG TACCTTGCGA
    GGTTTTGTCT ACTGCCGAAA TGAGGAGCTG GAGGCTGGGT GGGTTGCTTT TGGCAACAGC
    AAACTTCTTC ATCGGCCTGT CTCTTTTGAT AATAAGCCTC ACTCCCTTTT CCAGGTTGTG
    GATCAGCATA CCCTTCAGGT GTGTCAAATA ATCCCAATGC CAGTAAATCA CTTCCCAGTT
    GGCAGTACTA TGACCACCGT GCATCTTTCT TCAGATGGTA CATATTTCTA CTGGATTTGG
    TCTCCTGCAA GCCTCAATGA GAAAACACCA AAAGGCCACT CTGTCTTTAT GGATGTTTTT
    GAACTTATAG TAGAAAATGG TGTGTGTATA GCAAATCCTC TACAGGAACG AATTATATTA
    ATGCGAAAGG AAGGTGAATC TGCAAAGAGT ATTAATGAGA TGCTTCTGAG CAGGCTGTCC
    AGGTACAGAG CCTCCCCATC AGCAACATTG GCTGCTCTCA CTGGCTCTAC CATCTCTAAT
    ACTCTAAAAG AGGACCAAGC AGCTGCTAAC ACAAGCTGCG GGCTGCCACT GAAAATGTTA
    AGGAAGACTC CGATATACAC ATGTGGAACA TACCTGGTGA TGTTGGTCCC ACCCCCAGGA
    GGGTCAGGCA GTTCTGCAAC ACGCTCACTC TTTGGAGGAA CAAGTGCTTT GTCATCTTTA
    AAAATTCTTG CTTCAAGCTT GGTGTTCAAT ATTTCGGATG GGCAGTTCAC AAGCCGAGCA
    GATCTCATTG ATGCTGCAGG AAGTTCACTT GGACGTGGTG CTCTGGTACC AGGACTTGGT
    GCCTGTTATG ATACTATGAA TAACATGATA TGGACGTGCT CTAATGATTA CATCGATCAG
    TGGTGTAATC CTGGGAACCA GGCATTCCAC TGTGTCTGCC AGAGGCTGGG AGTGAGCCAT
    GTCATTACAG AGCCCAAAGG GGATGCAACA ACCACAAATG AGGTTATTAA CCAGTTACTG
    CACCATGTTG GTGCCATGTG TATCCACCAA CTCAATCTGC TTGCAGCCAG CAATAATCTT
    CCCATAACAA ACTTCTTGGG GAAGCAGCAT CCAATCGAAG CACATCATCT CAGCAGTATC
    TGTGACATTA TGGAGAAAGC TATGGTGAAC GGAGATACTT GTATAATACG CTGCATCCTT
    GTTGTCTTCC AGGTGGTTTT TAAATTTTTC TTCAGCCCAC AAACTGAAAG AAACAGAGAC
    ATTGTGAGGA GGTCGGGCCT ATTGCTTTGG CAGTTGTTGA TGGCACCAAG GGACCAGATT
    TGTGCGGAGA TTCAGAAGGA GGTTTGCCTG GCTATAAGCT CTGGTTTAAA TATCCTGTAT
    CCTGGAGAAG CAGAAATCAA TAATTTATTG AAATTGGTCT TAACTGAAGG AGAGAGAAAT
    AGTGGTCTTT CTCAACTTCG GGACGTCATC CTAACAAATC TAGCAGAACA GCTTCAGAAC
    AACAGATTTG GGAGTGAAGA TGATGATCAT TATAGACTAA ATGATGAGCT GTTGCACTAC
    ATTCTCAAGA TTGTTGTCCG AGAATCTTGT GTCTTAATCA CCAAGTGCCA AACTGTATCT
    AAAGATGATT TTCAAAGGCT TCTTTCAACT GTGCCTGCTG CATCTTCATG CTTGCGATAT
    CTGATGGCCG TACAGAACCA CCTCCTCAGT AACACTATTT TGATTAAACC TGATGAAAAT
    GATGACAGTG ACAGCTCCTT ACAGGGAGAG ACATTGAAGG TACAGGAGCT GAAGACCAGC
    GTTTTGTCTC TTGCTACACA AATTCTGACA GGATGTGATG AAGTCTTAGA AATGCTGCAG
    CAAGTCACTA CAGCACTAAT AAACAGTGAT ATTGCTGACA GAGAACAAAG ATTAAAAGGC
    CTGGAACAAA TTACGAAAGC TACTATGCTT GGTCATCTGC TTCCTGTCCT TCTGACATCC
    CTGATGCATC CAAATTTGCA AACTCTAACC ATGGCTGATG CCTTGATGCC TCAGCTGGTG
    CAGCTGGTTC TCTATACAAG TCAGACTGCA CTGCTGCTTA AAACTCAGTC TCCGGTTTTC
    TCAGAACTGA ACAGCTCTCC TGGTGGTGTG TCGGAACACA AAGGGAAATT GTTACATGAT
    GAGAGAATGC TGGAGGAGAA GGAAGAGCCA GGTTTTCTTA CAGGCTTGAA GATCCCTGCT
    CCTTGGGCTG CTGGGAAGAC TGTAGAAACT GTTCATCCTG TCAGGGATAA CTATAAATTT
    AAGGAAACTG TACACATACC AGGAGCCCGC TGTTTGTATC TTAGATTTGA CAACAGATGT
    TCTTCACAAT ATGATTACGA TAAGTCTAAC AGTGTATTTT ATGTTTTGAA GTTGGTGATC
    TATGCTGGTC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTAGTAAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGG AGTGGTCGTG AGCACAACAC TCCAGACAAA
    GCCATGTGGG GCTTTGCTTG CACTGTTCGA GCACAGGAGT CTTCAGAGGA TGTGTCAGGA
    GGCCTGCCAT TCTTAGTTGA CCTGGCACTT GGCCTTTCTG TGTTAGCTTG TTCTATGCTT
    CGGATTTTAT ACAATGGACC AGAAATTACC AAAGATGAAG AAACCTGTCA AGAGCTTCTA
    AGATCTAAAC TATTACAAAG GTGCCAATGG CAGGTGGAAG CTAATGGTGT AATATCTCCA
    GCCCTTACAC CGAGTCCTTC TCCACTGCCT CTTACTATAG ATGAGGACAG AGAATTTACT
    TATCCTTCTG ATGTGCTGGT GCCTCCCATT GGACACTATT TTGATCTGCC TAGGATTAGG
    CTGCCTCCAG GAATCATGAT AAAACTCAGG GAAATTTCTG GACGTGCTCG GCCTCAATTT
    AGACCTAGCA TAAAGGAAGT GATTCAGCCC GAGGTAATGG AGGAGATGGT AGTTTCATGT
    GTGATTAAAC ATTTGAATTT GGTTGATGCA CTCCAGTCCC TGATAAACTT CCAGTATCAG
    GAAGAACATG CTGAAGAATA TGATTTACTT TGTAAAATTA TGGGAGAGAC TTTTAAGAAG
    CTAAATGCCA TGGAGAGACA ATTACAGAGT GTGGCTGAGC TGGAACAGAA ATGGCAGAGT
    GAGGTTGATG ACGCCATGCA TGGGAAGCTG GAGAATAATG TTCCCTTCTT CTATGATTAC
    CACTTCAATG AGAGCAAGAT GAAAGAACTG GAACTTCTGT GTTCTATGAA GGAAGTTTCT
    TTTGATGGAA GTGATCTTGA GAATGTGGTC CTGGCATTAA GGGAGAAGTT CTTTCAAGAG
    GTGAATTCAC TGATACAGAA GACTTATCAT CCCCTAGCGA AAACAAAAGC ATTAGTTAAA
    AGCTTGATGA ACAGGGCAGA GCTGTTGCTG CATGTCACTA TTGCAGCGCA GTCTGGTATC
    ACAAGAAGTA TATCTGGAAC CCCTGCAGAG ACTCCAGCCT GTAAGTCTGC ATCTGAAACA
    AAAGTGATAT CTCATGCAGT GCGCCAGCCC GTCTTCCTTC GCAGTATGTC AGCACCATCT
    GACTTAGAGA TGATTGGTAA CGAAGATATT GAGTTTGCTC GATCAAGTCA AAGGCGACGC
    CATGTTACCA GCCACAGAAG TAGCTCTTTC ACTCTTCTGC AATCACTGAC CATTGAGGAC
    AGCAGAGATA AACCTACATA CAGTGTCCTA CTGGGGCAGC TCTTTGCTTT CATTGGAACT
    AATCCTGACC AAGCGGTATC CAGCAGCAGT TTTCTGCTCG CTGCTCAGAC AAGGTGGCGG
    CGTGGGAACA CAAGGAAGCA AGCCTTAGTG CATATGAGAG AGCTGCTGAC TGCGGCTGTT
    CGGGTTGGTG GGGTGACACA CCTCGTGGGC CCTGTGACCA TGGTACTTCA GGGAGGACCA
    AGAATTGAAG AGCTCACGTG TGGTGGGATG GTGGAACAAG TCCAGGAAGC TTTTGGGGAG
    ACTATGACAT CCGTGGTCTC GCTGTGTGCT CGCTACCCCA TTGCTTGTGC AAACAGCATT
    GGCCTCTTAT GCACTATACC TTACACAAGG AGTGAAGAGA AGTGTTTGGT ACGTAGTGGC
    CTGGTACAGC TTATGGACAG GCTGTGCAGC TTAAGCAGCC AGGCAGAATC CAGTTCAAAT
    GAAAAACAGA CCAAGAAGCA GAAGGTGGCC ACCATGGCTT GGGCTGCCTT TCAGGTGCTG
    GCCAATCGCT GTGTTGAATG GGAGAAAGAA GAAGGTGGTT CAACAGAAGC TGTTCACTCT
    GGTCTGGCTC GTCAGGTCTC CAGCCTTCTT ACCAATCACC TTGCACGAGC TACAGAGTGT
    TGTGGTAATC AAGCTGCAGG AAATGATGCA CTTCAAGACG TCCTCAGTCT TCTTAATGAC
    CTGTCGAGGA GTCACATAGG TAAAGCAATC CTGAGCCAGC CAGCCTGTGT GTCCAAACTT
    CTGTCACTTC TCTTGGATCA ACGTCCCTCT CCAAAGTTGG TTCTTATAAT CCTTCAGTTG
    TGTCGTGCTG CTCTACCTCT CATGAGTGTT GAAGACTGTG GAAATGTAGA ACTGCCTCCC
    TGGAGTTACT CTGTGCCCTC CCTGAACAGT GAACAAGATG ATCCCAGCGA CCCAGCCTCC
    AAGATTGCAT CTCTGCTCTT AGCAAAGCTG GCAGATTATG TAGTACCAGG ATGCCAGACA
    GTTCTTTCTC CAACTGCTTC TGAACCAGAT ACTGCTTTAG CAAAAAGCAG CCCTAAGAAT
    TCTATAAAAG GCGATAAAGA CCCTGGAGAA GAAAGTGAGG CAGTGGATGG CAAACTTTCT
    ATTTTCATCC ATAAACGGGA AGACCAGTCA TCCCACGAAG TGCTTCAGCC ATTGCTGAGT
    AGCTCCGAAG GTCGCCCATT CCGACTAGGA ACTGGAGCCA ACATGGAGAA GGTGGTGAAA
    ATGGATCGTG ACATGACTAA AAGTGGCTGC TGTGAAGTTA TCACAGAAGA AGCTGCTGCA
    GCTCTTCGCA AGGCAACTAA GTGGGCTCAG TCTGGTCTGA TAGTTAGTGT TGGGCCACCC
    ATAGAAACTG TCAATCCAGA AGCAACCAGT GGGATGTCCT CTGGGGACAA AAAGAAGACT
    GCACAGACAT CTATTTGCAG AGAGAGAAAT TCTGAGCTTG CCAGGACTGA TCCTGTAAGA
    CCATTCATCA GTGGTCATGT TGCAAATAGC ATGGCAGCAG AAGTGATCGC TTTGCTACAT
    AGCTTGTTGA TGGCACCAGA ATCAAATGCG GCTCAAATAT GGACAACAAC TGCTGAAAAA
    GTTCTGTCTC GGGCTCTGAT GTACATTCCA CAACTCGGGA AATACGCAGA GAGTATTTTG
    GAGAATGGAA GCAGCAGTGG AAGGAAGCTT GCTAAACTTC AAAGAATTGC TCGACAGGCA
    GTAGCTGCTC TTTGTGCTCT AGGTGGCTTT AAGGAGACAA TTAAAATCGG TTCTGAAGTT
    CAGGTTTTAG GTAAAGGAAT AGCAGGAAGC ATTGGAGTTG TGGCATCTAT TAATGAACAA
    GAAGGTATAG CAACGGTCAA ATTTCCGCCT ACCACTATAG ACAGTAGAAA GACCTCACAA
    GCATCAGACA CTTTAACTAT TCCGTTATCT AGGCTCTGTG TTCCAAGATC TGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTATC TATTCACACC TCACTTCCTG CAACAGGAGA TGGCTCAACT
    CCAGTAATGG CAGTGGTCCG GCTCCTTGCT GAAATAAGAA CCAGAGCATG CCTGGTGATG
    GCTCAGCTGT TAGAAGACAG TTCATTCTGT GAAGAATTCA TTCAACAGTG CCCAGCTGCT
    GTAGAGGTTC TTAATTTGGT AGCCCAGGAG TGCAGCCCTG GAGAGAGGCT CACTGTAGTA
    GAAATGCAGT GTGAAAGGTT AAGGATGTTG TATCGTGACT GTGCTCGACC CCCACCTCCT
    CCACTGCAGG CAGACAGAAG GCAGCCCAAA GAAATCACTT GGAGCCCATC AAGAGTGTTT
    CCCCCAGTAC GAGCATGCAT GTTTTCATCA CATCTTGCTG CTGTGACCTT CCTGGCTGAT
    CCAAGTGCAG GTGGGGGACT TCCTCGGGGA ACGTTCATTT ATGCCACTTC TCCAGTTCCC
    GTGCAGGCAC CATCATTCTA CTGGGAAATT GAAATAGTTT CCTATGGAGA TACAGATGAT
    GACACTGGTC CAATAGTTTC ATTTGGATTC GCTACAGAGG CAGAAAAACG GGATGGGGCT
    TGGACAAACC CAGTGGGCAC CTGTCTTTTT CATAACAATG GGCGAGCAGT GCATTACAAT
    GGCTCCAGCT TGTTACAGTG GAAGAGTGTT CGATTGGATG TGACTCTTTC TCCTGGTGAT
    GTGGCTGGAA TTGGATGGGA AAGAGCAGAA GGAACTCCAC CCCCACCAGG GCAGCCTGCT
    AAAGGCAGAG TTTATTTTAC TTATTGCGGG CAGCGACTTG GGCCATACCT AGAGGATGTC
    TCTGGTGGAA TGTGGCCAAT TGTTCACATA CAGAAAAAGA ACACAAAAGT CCGTGCTAAT
    TTTGGATCTC GCCAGTTTGC CTATGCCGAA GGCCAAGCTC ACAGGAATGC TGCTGATCTG
    TGTATTGACC TAGCTGAAGA GATAAGTGCC AACTTTGAGG CATTGCCATT TGCTATGGCT
    TCTGATAGCG ATAACGACGC TGGCACCAGC ATAGCTTCTG ATCCTGGGAC TCACGGACCT
    CCCTGTAGAA TTGCTGCTGT TGCTACGGCT CAACAACAAT ATGACAGTGA CACATCTTGT
    CACTATAAAA TGGAACTAAG CTATGAGAAC TTCATCACAT CAGGGCCTGA TCCTCATCCC
    CCTCCTATTG CAGATGATGA AAGTGATGAT GACGACGATG ATGATATTCC CAGGGAGGAC
    CACTATGCCC TGCTGGTTAA AGCCTGGGAA ACTAAAGTTT TCCCTACAAT TAGAAGAAGA
    TTCCGTAATG AGGCTGAGAG GAAATCTGGG CTGGATCAAA TTAAAGGAGC CTTACAGTTA
    GGAATGGTTG ATATAGCACG ACAAACTGTA GAATTCTTGT ATGAGGAAAA CGGTGGCATC
    CCGAGAGACC TCTACCTTCC TACCATTGAG GATATCAAAG ATGAAGCAAA CAAGTTTACA
    ATCGATAAAG TACGAAAAGG TCTCACTGTT GTAACTCGCT CTCCTGACAG TAACAACGTT
    ACCAGCAGTG CTGTTGGAAC AGCTTTACCC AAGTTTGCTA TTCGGGGAAT GCTGAAGACA
    TTTGGTCTTC ATGGTGTTGT CCTGGATGTT GATTCAGTGA ATGAATTGGT ACAAGTAGAG
    ACCTATCTCA GAAGTGAGGG AGTTCTGGTA AGATATTGGT ATCCTATTGA CATGTTAGAA
    AGGCCACCTG CTGGTTATAG GAGGACTGCA ACAAATGGAT TGGTTACCCT GGACAATACC
    AATATCCAAA TCCACAGAGA GCTTTTGAGA AGTGAAGCTG CACTGGCCAA GCTGTACTGC
    CGCATGGCTC TCCTCAATAT CTTTGCCCCC AAATCTCCAC ATATGTTCAC CCGTCTGTTT
    CATATCCCCG CTATCCGTGA TATCACTCTG GAGCACCTGC AGTTGCTATC CAACCAACTT
    CTCGCACCAC CTCTTCCTGA TGGAACAATC AGTTCAAGTT CCATTCTTTT GGCTCAGTCA
    CTGCAACACT GCATCCATTC CCAGAACTGT GCTGCCACAG ACCTCTTCTA CCAGGGCAAT
    TCACAAGCCA TCAGAGAGTG GCTTACTGTT GCCATCACTC GGACACTGCA TCAAGGAGAG
    GAGAGCCTGT TAGACCTCAC TAAGCAGATC TGCTCTTTTT TGCAGACTGC ACCAGAACAG
    TTTCCTGCTG AAGAATTCCC AATTTCAGAA TCCAAAGTCA ACATGGATGT TAATTTTCCT
    GGGGCTGCGT TTGTAATTGT GTCCTGTAAA GAAAGCCAGT CTGGATTCCG CAAAGATTCT
    TCCCTGTATA AAGCTCCTTG GGCTCGGGTG CTTGTGTACG GGCTGGGGCA CAAAGTTAAG
    AGGAATGGCC AGTTAAACCT GATTGAGGCA GTGTGTTACC CTCGAGACGC CTCTCCGACC
    AACACAGGCC TAACACCTCC CCCAACAACC AATCAGTACC CTTCAGTGAT CACTCCTACA
    GACAAAGTGC ACATCAAACT AGGAGTGTCT CCTCCTCCTG GAGCTGTGCT GGTCTTGCAT
    TCTCTTCCCC TTGAGTTCCC CCTGGCAATG GCATTTACAG AACAGCTGTT AACTTGGAAA
    CTGGAAGATG TGGATGGGAA ATCAGAAGAT GAACTGGATA CTATTCCTCC TTCAGTTCTT
    TTGCAAGTAG TGGAATTTTT AGGGAACTTT CTGTGGACAA CTGACATGGC AGCATCTGTA
    AAGGAATTGA TATTTCATCT CTTGGCTGAA TTGCTTCGCA AAATTCACAA CCTGGAGCAG
    AAGAAAAGTC CTGCAGGATT ATCATCTTCT ATTGCCCTTC AGCTGAATCC CTGTCTAGCT
    ATGCTTATGG CCCTGCAGTC AGAACTCCAT AAGCTGTACG ATGAGGAAAC ACAAAACTGG
    GTGTCTGGAA ATGCATGCGG TGGTTCTGGT GTTGGAGCTG CTGACCAAGG AAGATTTTCA
    ACATACTTCC ATGCTCTGAT GGAAGTTTGC CTTGCTGTAG CAGAAGTAAC TCTACCTATC
    AATATGAGCG TAACAGCTAA TGTGATATCT TCAACGAGTG CACCAAATCT TAGTGACTCT
    TCCTCCTCAT CTTCATCTTC TCCAGGACAG ACACCGCAGA GTCCAAGCTT ATTGTCAAAG
    AGGAAAAAAG TCAAAATGAA GAGAGAAAAG ACATCGTCTT CAGGCAAACG ATCTTCATCC
    AGAGCAGCTG AAACGGATGC TGCAATCCTC AGTATAGGAG GAAGCAAGCC TGAGGATATG
    CTATGGTTTC ATAGAGCACT GACTTTGCTT ATCATTCTCA GACACCTAAC GAAAAAGGAT
    CCGCAAGGGT TGGGTGTGAC AAACGATGCT GTAGCAGATG CATGTCAGGC ACTGGTAGGA
    CCCACAGCTC ATAGCCGTTT GCTTGTCATT TCTGGAATAC CAACTCACTT GGAGGAGAGT
    ACTGTCAGAA ATGCAATCAG GAAGGCGTGT AATGCGCATG GTGGAGTCTT CAAGGATGAA
    ATCTACATCC CTCTGCAGGA AGAGGATCCC AAAAAAATCA AGGACAAAGC AGAAGGAGGA
    GAATGCAGAA CTGAGCTGGA AAAGCCAACT GTTTCAAGTA GTGCGGACAG TCTGGATATC
    AGCAGCTCTA GCAGTGTCAC ACCTGCTATG AGCGTTAGTG CGTCAGCATC AACGAGCCAG
    GCTTCCCTGT GCAGCTCACA GGGTATCTCC CGGACTGTTA GTGACATCTC TGTTGATCAG
    TACCAGGCTG GGCTGGAACT GGCCATCCCA CCAGGACTGC TGGAGCCACA CGTTTCCAGC
    CAGGAGAGCT TGGATCTGTC GCACTGCAGT ACTGGGAGCC TTGGCAGCCT TGGCAGCCTC
    GGGGAGCCGC TTGACAATGT AGAGACAGCA TCAGTGTCTG ATGTCGGGTC GATGTACACG
    GTCACATCTC TGGACAACCA GCAACTTCTG TCACGGCCTA TCAAAGGTTT TGCTGTGGTG
    GAGATACGAT CAAGAGCTAA AATAGAGAAA ATCCGAGCCA GCCTATTTAA CAGTAGTGAT
    CTCATTGGTT TGTCAAGTCT GGATGGTGAA GACGAACTGA TGGAAATGTC AACTGAAGAG
    ATTTTAACTG TTTCTACTGT AAATCAGAGT TTATTTGACA CACAAGGGAG CCCGGCGCTA
    GAAGACTACT TCAATGATAA GTCAATAAAA GGTGAGAAAT TGGTTCCTGG TGCTCGGGAG
    GTCCTAACAG AAATATTTAA AAGCTGTGTC CATTCTGAGC AAATGCTGAG CCTGACTCCA
    ACAAAACCAA TCAAGGTTGC TGATATCTAT CTTAGTAAAG AGCAGATAAA TTCTCAGACT
    CCTGGAAACC TTCTTCATGT CTTCTTCACA AATGTCCGTC CTCCAAAGAA GGTGCTGGAG
    GACCAGCTTA CTCAGATTCT AAGGAAATAC GGTGTGCCCA AGCCGAAATT TGACAAGAGC
    AAATACAACA AGGCAGGCAA AGAACAGCAT CCGGTGAAGG TTGTGAGCAC CAAGCGCCCA
    GTTACAAAAC CACCCACAAA AGAAAAGTCT GTACTCAACA GCGTCAGCCG AACTGCTTTG
    AGTGAGAAGA AACCTACTGT AAAGCCAAAG TCTCCTGAGA AGAGCAAACC TGAAGAGAAG
    GACCCTGAAA AGTCTCCCAC AAAGAAACAA GAAGTTCCTG AGGAGAAGTA CCTGACACTG
    GATGGATTTC ACAGATTTGT CATTGACCGA TCTAAGCAGG ATATCCGTAG TGTGTGGAGA
    GCTATTCTGT CCTGTGGATA TGATCTGCAT TTTGAAAGAT GTGCCTGCAT TGATGCAAGA
    CATGCACAGA AAGCATCTCG GAAGTGGACT CTGGAAATGG ACGTGGCTCT CGTTCAGTAC
    ATTAACAGGC TTTGTCGTCA CCTCGCAATT ACACCAGCAC GACTCCATCC ACATGAAGTA
    TATTTGGATC CAGCTGATGC AGCAGATCCT AGAATCTCCT GTCTGTTGAA TGTTCCCGTT
    GAAAGTCTCC GCCTGCGGTT TGCACTGCTT CAGTCTCTCA ATACGACGTT AGAGACGTTT
    TTCTTACCGC TGGTAGAGCT TCGGCAGACT GAGATGTACA GTAACAGCAT TGCTGCGCTG
    CTGCAGGAGG CCAAAGGTTT AATCTTCTAT GACACAAAAA TAACCGTAAT GAACAGAGTG
    CTGAATGCCA CTGTCCAAAG AACTGCTGAT CACGCAGCCC CAGAAATCAC CTTGGATCCC
    TTGGAAATAG TTGGAGGGGA GATCCGTTCT TCAGAAAATT CCTACTTCTG CCAGGCAGCA
    AGGCAGCTGG CCTGTGTGCC ATCCTCACAG CTCTGCGTCA AACTCGCCAG TGGTGGGGAC
    CCTACGTACG CGTTCAACAT CCGGTTCACA GGGGAGGAGG TCCATGGAAC AAGTGGATCT
    TTCCGTCACT TCCTCTGGCA AGTTTGTAAG GAGCTGCAGA GCTCCTCGCT CTCTCTTCTC
    CTGTTGTGTC CAAGCTCTGC GGTCAACAAG AACAAGGGGA AGTACATTTT GACACCTAGC
    CCCATAACCT ATGCAGAGGA GCAGCTGTTC CATTTCTTTG GGCAGCTTCT GGGCATTGCA
    ATTCGAGCAG ATGTTCCCCT GCCACTCGAC CTCCTGCCCT CATTCTGGAA GACTCTGGTA
    GGAGAGCCGC TGGATCCTGA TGTTGATCTC CAGGAAGCTG ATATCCTCAC CTACAACTAC
    ATTAAAAAGT TTGAAAATAT AAATGATGAA ACTGAACTGG AAGCTCTGTG TGCAGAAATT
    GCTTCTCAGC ATTTAGCAAC AGAGAGCCCT GACTGTCCTA ATAAGCCATG CTGCAAATTC
    ACCTACCTGA CCATGACAGG GGAAGAGGTG GAACTCTGTC CCAGGGGCAG GCACGTCCCA
    GTGGGATGGG AGAACAAGGA CGTGTATGCA GCTGCAATCA GGAGCTTGAG AATGCGTGAG
    CTGCAGACTC CAGAGTGCAT GACTGCAGTG CGTGCTGGGC TTGGGTCCAT CATTCCCCTG
    CAGCTTCTGA CCACGCTGAC CCCTCTTGAG ATGGAGCTGA GGACATGTGG GCTTCCATAC
    ATCAACCTGG AGTTTCTCAA GGCGCACACG ATGTACCAGG TGGGACTGAT GGAAACTGAT
    CAGCACATTG AGTTTTTTTG GAGCGCGCTG GAGATGTTCA CTCAGGAGGA GCTCTGCAAG
    TTCATAAAGT TTGCCTGTAA CCAGGAGCGG ATCCCCTTCA CGTGTCCTTG CAAGGACGGC
    GGTCCCGATA CCGCCCACGT CCCGCCTTAC CCCATGAAAA TAGCCCCCCC TGATGGAGCT
    GCAGGTTCTC CGGACTCTCG GTACATCCGT GTGGAAACGT GCATGTTCAT GATCAAACTT
    CCTCAGTACT CATCACTGGA CATCATGCTG GAGAAGCTCC GATACGCCAT CCACTACCGG
    GAGGATCCTC TCAGCGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131027.1
    CDS263..13543
    Translation

    Target ORF information:

    RefSeq Version XM_015275541.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275541.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGACTT AGGACGTTGT CTTGCAGCAG ATGGCCTCTA CCTTTATACA
    ACAAATTCAG TGGGAAGAGG AATAAGCAAA TTAGGATCTG GATTACATGG TACCTTGCGA
    GGTTTTGTCT ACTGCCGAAA TGAGGAGCTG GAGGCTGGGT GGGTTGCTTT TGGCAACAGC
    AAACTTCTTC ATCGGCCTGT CTCTTTTGAT AATAAGCCTC ACTCCCTTTT CCAGGTTGTG
    GATCAGCATA CCCTTCAGGT GTGTCAAATA ATCCCAATGC CAGTAAATCA CTTCCCAGTT
    GGCAGTACTA TGACCACCGT GCATCTTTCT TCAGATGGTA CATATTTCTA CTGGATTTGG
    TCTCCTGCAA GCCTCAATGA GAAAACACCA AAAGGCCACT CTGTCTTTAT GGATGTTTTT
    GAACTTATAG TAGAAAATGG TGTGTGTATA GCAAATCCTC TACAGGAACG AATTATATTA
    ATGCGAAAGG AAGGTGAATC TGCAAAGAGT ATTAATGAGA TGCTTCTGAG CAGGCTGTCC
    AGGTACAGAG CCTCCCCATC AGCAACATTG GCTGCTCTCA CTGGCTCTAC CATCTCTAAT
    ACTCTAAAAG AGGACCAAGC AGCTGCTAAC ACAAGCTGCG GGCTGCCACT GAAAATGTTA
    AGGAAGACTC CGATATACAC ATGTGGAACA TACCTGGTGA TGTTGGTCCC ACCCCCAGGA
    GGGTCAGGCA GTTCTGCAAC ACGCTCACTC TTTGGAGGAA CAAGTGCTTT GTCATCTTTA
    AAAATTCTTG CTTCAAGCTT GGTGTTCAAT ATTTCGGATG GGCAGTTCAC AAGCCGAGCA
    GATCTCATTG ATGCTGCAGG AAGTTCACTT GGACGTGGTG CTCTGGTACC AGGACTTGGT
    GCCTGTTATG ATACTATGAA TAACATGATA TGGACGTGCT CTAATGATTA CATCGATCAG
    TGGTGTAATC CTGGGAACCA GGCATTCCAC TGTGTCTGCC AGAGGCTGGG AGTGAGCCAT
    GTCATTACAG AGCCCAAAGG GGATGCAACA ACCACAAATG AGGTTATTAA CCAGTTACTG
    CACCATGTTG GTGCCATGTG TATCCACCAA CTCAATCTGC TTGCAGCCAG CAATAATCTT
    CCCATAACAA ACTTCTTGGG GAAGCAGCAT CCAATCGAAG CACATCATCT CAGCAGTATC
    TGTGACATTA TGGAGAAAGC TATGGTGAAC GGAGATACTT GTATAATACG CTGCATCCTT
    GTTGTCTTCC AGGTGGTTTT TAAATTTTTC TTCAGCCCAC AAACTGAAAG AAACAGAGAC
    ATTGTGAGGA GGTCGGGCCT ATTGCTTTGG CAGTTGTTGA TGGCACCAAG GGACCAGATT
    TGTGCGGAGA TTCAGAAGGA GGTTTGCCTG GCTATAAGCT CTGGTTTAAA TATCCTGTAT
    CCTGGAGAAG CAGAAATCAA TAATTTATTG AAATTGGTCT TAACTGAAGG AGAGAGAAAT
    AGTGGTCTTT CTCAACTTCG GGACGTCATC CTAACAAATC TAGCAGAACA GCTTCAGAAC
    AACAGATTTG GGAGTGAAGA TGATGATCAT TATAGACTAA ATGATGAGCT GTTGCACTAC
    ATTCTCAAGA TTGTTGTCCG AGAATCTTGT GTCTTAATCA CCAAGTGCCA AACTGTATCT
    AAAGATGATT TTCAAAGGCT TCTTTCAACT GTGCCTGCTG CATCTTCATG CTTGCGATAT
    CTGATGGCCG TACAGAACCA CCTCCTCAGT AACACTATTT TGATTAAACC TGATGAAAAT
    GATGACAGTG ACAGCTCCTT ACAGGGAGAG ACATTGAAGG TACAGGAGCT GAAGACCAGC
    GTTTTGTCTC TTGCTACACA AATTCTGACA GGATGTGATG AAGTCTTAGA AATGCTGCAG
    CAAGTCACTA CAGCACTAAT AAACAGTGAT ATTGCTGACA GAGAACAAAG ATTAAAAGGC
    CTGGAACAAA TTACGAAAGC TACTATGCTT GGTCATCTGC TTCCTGTCCT TCTGACATCC
    CTGATGCATC CAAATTTGCA AACTCTAACC ATGGCTGATG CCTTGATGCC TCAGCTGGTG
    CAGCTGGTTC TCTATACAAG TCAGACTGCA CTGCTGCTTA AAACTCAGTC TCCGGTTTTC
    TCAGAACTGA ACAGCTCTCC TGGTGGTGTG TCGGAACACA AAGGGAAATT GTTACATGAT
    GAGAGAATGC TGGAGGAGAA GGAAGAGCCA GGTTTTCTTA CAGGCTTGAA GATCCCTGCT
    CCTTGGGCTG CTGGGAAGAC TGTAGAAACT GTTCATCCTG TCAGGGATAA CTATAAATTT
    AAGGAAACTG TACACATACC AGGAGCCCGC TGTTTGTATC TTAGATTTGA CAACAGATGT
    TCTTCACAAT ATGATTACGA TAAGTCTAAC AGTGTATTTT ATGTTTTGAA GTTGGTGATC
    TATGCTGGTC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTAGTAAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGG AGTGGTCGTG AGCACAACAC TCCAGACAAA
    GCCATGTGGG GCTTTGCTTG CACTGTTCGA GCACAGGAGT CTTCAGAGGA TGTGTCAGGA
    GGCCTGCCAT TCTTAGTTGA CCTGGCACTT GGCCTTTCTG TGTTAGCTTG TTCTATGCTT
    CGGATTTTAT ACAATGGACC AGAAATTACC AAAGATGAAG AAACCTGTCA AGAGCTTCTA
    AGATCTAAAC TATTACAAAG GTGCCAATGG CAGGTGGAAG CTAATGGTGT AATATCTCCA
    GCCCTTACAC CGAGTCCTTC TCCACTGCCT CTTACTATAG ATGAGGACAG AGAATTTACT
    TATCCTTCTG ATGTGCTGGT GCCTCCCATT GGACACTATT TTGATCTGCC TAGGATTAGG
    CTGCCTCCAG GAATCATGAT AAAACTCAGG GAAATTTCTG GACGTGCTCG GCCTCAATTT
    AGACCTAGCA TAAAGGAAGT GATTCAGCCC GAGGTAATGG AGGAGATGGT AGTTTCATGT
    GTGATTAAAC ATTTGAATTT GGTTGATGCA CTCCAGTCCC TGATAAACTT CCAGTATCAG
    GAAGAACATG CTGAAGAATA TGATTTACTT TGTAAAATTA TGGGAGAGAC TTTTAAGAAG
    CTAAATGCCA TGGAGAGACA ATTACAGAGT GTGGCTGAGC TGGAACAGAA ATGGCAGAGT
    GAGGTTGATG ACGCCATGCA TGGGAAGCTG GAGAATAATG TTCCCTTCTT CTATGATTAC
    CACTTCAATG AGAGCAAGAT GAAAGAACTG GAACTTCTGT GTTCTATGAA GGAAGTTTCT
    TTTGATGGAA GTGATCTTGA GAATGTGGTC CTGGCATTAA GGGAGAAGTT CTTTCAAGAG
    GTGAATTCAC TGATACAGAA GACTTATCAT CCCCTAGCGA AAACAAAAGC ATTAGTTAAA
    AGCTTGATGA ACAGGGCAGA GCTGTTGCTG CATGTCACTA TTGCAGCGCA GTCTGGTATC
    ACAAGAAGTA TATCTGGAAC CCCTGCAGAG ACTCCAGCCT GTAAGTCTGC ATCTGAAACA
    AAAGTGATAT CTCATGCAGT GCGCCAGCCC GTCTTCCTTC GCAGTATGTC AGCACCATCT
    GACTTAGAGA TGATTGGTAA CGAAGATATT GAGTTTGCTC GATCAAGTCA AAGGCGACGC
    CATGTTACCA GCCACAGAAG TAGCTCTTTC ACTCTTCTGC AATCACTGAC CATTGAGGAC
    AGCAGAGATA AACCTACATA CAGTGTCCTA CTGGGGCAGC TCTTTGCTTT CATTGGAACT
    AATCCTGACC AAGCGGTATC CAGCAGCAGT TTTCTGCTCG CTGCTCAGAC AAGGTGGCGG
    CGTGGGAACA CAAGGAAGCA AGCCTTAGTG CATATGAGAG AGCTGCTGAC TGCGGCTGTT
    CGGGTTGGTG GGGTGACACA CCTCGTGGGC CCTGTGACCA TGGTACTTCA GGGAGGACCA
    AGAATTGAAG AGCTCACGTG TGGTGGGATG GTGGAACAAG TCCAGGAAGC TTTTGGGGAG
    ACTATGACAT CCGTGGTCTC GCTGTGTGCT CGCTACCCCA TTGCTTGTGC AAACAGCATT
    GGCCTCTTAT GCACTATACC TTACACAAGG AGTGAAGAGA AGTGTTTGGT ACGTAGTGGC
    CTGGTACAGC TTATGGACAG GCTGTGCAGC TTAAGCAGCC AGGCAGAATC CAGTTCAAAT
    GAAAAACAGA CCAAGAAGCA GAAGGTGGCC ACCATGGCTT GGGCTGCCTT TCAGGTGCTG
    GCCAATCGCT GTGTTGAATG GGAGAAAGAA GAAGGTGGTT CAACAGAAGC TGTTCACTCT
    GGTCTGGCTC GTCAGGTCTC CAGCCTTCTT ACCAATCACC TTGCACGAGC TACAGAGTGT
    TGTGGTAATC AAGCTGCAGG AAATGATGCA CTTCAAGACG TCCTCAGTCT TCTTAATGAC
    CTGTCGAGGA GTCACATAGG TAAAGCAATC CTGAGCCAGC CAGCCTGTGT GTCCAAACTT
    CTGTCACTTC TCTTGGATCA ACGTCCCTCT CCAAAGTTGG TTCTTATAAT CCTTCAGTTG
    TGTCGTGCTG CTCTACCTCT CATGAGTGTT GAAGACTGTG GAAATGTAGA ACTGCCTCCC
    TGGAGTTACT CTGTGCCCTC CCTGAACAGT GAACAAGATG ATCCCAGCGA CCCAGCCTCC
    AAGATTGCAT CTCTGCTCTT AGCAAAGCTG GCAGATTATG TAGTACCAGG ATGCCAGACA
    GTTCTTTCTC CAACTGCTTC TGAACCAGAT ACTGCTTTAG CAAAAAGCAG CCCTAAGAAT
    TCTATAAAAG GCGATAAAGA CCCTGGAGAA GAAAGTGAGG CAGTGGATGG CAAACTTTCT
    ATTTTCATCC ATAAACGGGA AGACCAGTCA TCCCACGAAG TGCTTCAGCC ATTGCTGAGT
    AGCTCCGAAG GTCGCCCATT CCGACTAGGA ACTGGAGCCA ACATGGAGAA GGTGGTGAAA
    ATGGATCGTG ACATGACTAA AAGTGGCTGC TGTGAAGTTA TCACAGAAGA AGCTGCTGCA
    GCTCTTCGCA AGGCAACTAA GTGGGCTCAG TCTGGTCTGA TAGTTAGTGT TGGGCCACCC
    ATAGAAACTG TCAATCCAGA AGCAACCAGT GGGATGTCCT CTGGGGACAA AAAGAAGACT
    GCACAGACAT CTATTTGCAG AGAGAGAAAT TCTGAGCTTG CCAGGACTGA TCCTGTAAGA
    CCATTCATCA GTGGTCATGT TGCAAATAGC ATGGCAGCAG AAGTGATCGC TTTGCTACAT
    AGCTTGTTGA TGGCACCAGA ATCAAATGCG GCTCAAATAT GGACAACAAC TGCTGAAAAA
    GTTCTGTCTC GGGCTCTGAT GTACATTCCA CAACTCGGGA AATACGCAGA GAGTATTTTG
    GAGAATGGAA GCAGCAGTGG AAGGAAGCTT GCTAAACTTC AAAGAATTGC TCGACAGGCA
    GTAGCTGCTC TTTGTGCTCT AGGTGGCTTT AAGGAGACAA TTAAAATCGG TTCTGAAGTT
    CAGGTTTTAG GTAAAGGAAT AGCAGGAAGC ATTGGAGTTG TGGCATCTAT TAATGAACAA
    GAAGGTATAG CAACGGTCAA ATTTCCGCCT ACCACTATAG ACAGTAGAAA GACCTCACAA
    GCATCAGACA CTTTAACTAT TCCGTTATCT AGGCTCTGTG TTCCAAGATC TGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTATC TATTCACACC TCACTTCCTG CAACAGGAGA TGGCTCAACT
    CCAGTAATGG CAGTGGTCCG GCTCCTTGCT GAAATAAGAA CCAGAGCATG CCTGGTGATG
    GCTCAGCTGT TAGAAGACAG TTCATTCTGT GAAGAATTCA TTCAACAGTG CCCAGCTGCT
    GTAGAGGTTC TTAATTTGGT AGCCCAGGAG TGCAGCCCTG GAGAGAGGCT CACTGTAGTA
    GAAATGCAGT GTGAAAGGTT AAGGATGTTG TATCGTGACT GTGCTCGACC CCCACCTCCT
    CCACTGCAGG CAGACAGAAG GCAGCCCAAA GAAATCACTT GGAGCCCATC AAGAGTGTTT
    CCCCCAGTAC GAGCATGCAT GTTTTCATCA CATCTTGCTG CTGTGACCTT CCTGGCTGAT
    CCAAGTGCAG GTGGGGGACT TCCTCGGGGA ACGTTCATTT ATGCCACTTC TCCAGTTCCC
    GTGCAGGCAC CATCATTCTA CTGGGAAATT GAAATAGTTT CCTATGGAGA TACAGATGAT
    GACACTGGTC CAATAGTTTC ATTTGGATTC GCTACAGAGG CAGAAAAACG GGATGGGGCT
    TGGACAAACC CAGTGGGCAC CTGTCTTTTT CATAACAATG GGCGAGCAGT GCATTACAAT
    GGCTCCAGCT TGTTACAGTG GAAGAGTGTT CGATTGGATG TGACTCTTTC TCCTGGTGAT
    GTGGCTGGAA TTGGATGGGA AAGAGCAGAA GGAACTCCAC CCCCACCAGG GCAGCCTGCT
    AAAGGCAGAG TTTATTTTAC TTATTGCGGG CAGCGACTTG GGCCATACCT AGAGGATGTC
    TCTGGTGGAA TGTGGCCAAT TGTTCACATA CAGAAAAAGA ACACAAAAGT CCGTGCTAAT
    TTTGGATCTC GCCAGTTTGC CTATGCCGAA GGCCAAGCTC ACAGGAATGC TGCTGATCTG
    TGTATTGACC TAGCTGAAGA GATAAGTGCC AACTTTGAGG CATTGCCATT TGCTATGGCT
    TCTGATAGCG ATAACGACGC TGGCACCAGC ATAGCTTCTG ATCCTGGGAC TCACGGACCT
    CCCTGTAGAA TTGCTGCTGT TGCTACGGCT CAACAACAAT ATGACAGTGA CACATCTTGT
    CACTATAAAA TGGAACTAAG CTATGAGAAC TTCATCACAT CAGGGCCTGA TCCTCATCCC
    CCTCCTATTG CAGATGATGA AAGTGATGAT GACGACGATG ATGATATTCC CAGGGAGGAC
    CACTATGCCC TGCTGGTTAA AGCCTGGGAA ACTAAAGTTT TCCCTACAAT TAGAAGAAGA
    TTCCGTAATG AGGCTGAGAG GAAATCTGGG CTGGATCAAA TTAAAGGAGC CTTACAGTTA
    GGAATGGTTG ATATAGCACG ACAAACTGTA GAATTCTTGT ATGAGGAAAA CGGTGGCATC
    CCGAGAGACC TCTACCTTCC TACCATTGAG GATATCAAAG ATGAAGCAAA CAAGTTTACA
    ATCGATAAAG TACGAAAAGG TCTCACTGTT GTAACTCGCT CTCCTGACAG TAACAACGTT
    ACCAGCAGTG CTGTTGGAAC AGCTTTACCC AAGTTTGCTA TTCGGGGAAT GCTGAAGACA
    TTTGGTCTTC ATGGTGTTGT CCTGGATGTT GATTCAGTGA ATGAATTGGT ACAAGTAGAG
    ACCTATCTCA GAAGTGAGGG AGTTCTGGTA AGATATTGGT ATCCTATTGA CATGTTAGAA
    AGGCCACCTG CTGGTTATAG GAGGACTGCA ACAAATGGAT TGGTTACCCT GGACAATACC
    AATATCCAAA TCCACAGAGA GCTTTTGAGA AGTGAAGCTG CACTGGCCAA GCTGTACTGC
    CGCATGGCTC TCCTCAATAT CTTTGCCCCC AAATCTCCAC ATATGTTCAC CCGTCTGTTT
    CATATCCCCG CTATCCGTGA TATCACTCTG GAGCACCTGC AGTTGCTATC CAACCAACTT
    CTCGCACCAC CTCTTCCTGA TGGAACAATC AGTTCAAGTT CCATTCTTTT GGCTCAGTCA
    CTGCAACACT GCATCCATTC CCAGAACTGT GCTGCCACAG ACCTCTTCTA CCAGGGCAAT
    TCACAAGCCA TCAGAGAGTG GCTTACTGTT GCCATCACTC GGACACTGCA TCAAGGAGAG
    GAGAGCCTGT TAGACCTCAC TAAGCAGATC TGCTCTTTTT TGCAGACTGC ACCAGAACAG
    TTTCCTGCTG AAGAATTCCC AATTTCAGAA TCCAAAGTCA ACATGGATGT TAATTTTCCT
    GGGGCTGCGT TTGTAATTGT GTCCTGTAAA GAAAGCCAGT CTGGATTCCG CAAAGATTCT
    TCCCTGTATA AAGCTCCTTG GGCTCGGGTG CTTGTGTACG GGCTGGGGCA CAAAGTTAAG
    AGGAATGGCC AGTTAAACCT GATTGAGGCA GTGTGTTACC CTCGAGACGC CTCTCCGACC
    AACACAGGCC TAACACCTCC CCCAACAACC AATCAGTACC CTTCAGTGAT CACTCCTACA
    GACAAAGTGC ACATCAAACT AGGAGTGTCT CCTCCTCCTG GAGCTGTGCT GGTCTTGCAT
    TCTCTTCCCC TTGAGTTCCC CCTGGCAATG GCATTTACAG AACAGCTGTT AACTTGGAAA
    CTGGAAGATG TGGATGGGAA ATCAGAAGAT GAACTGGATA CTATTCCTCC TTCAGTTCTT
    TTGCAAGTAG TGGAATTTTT AGGGAACTTT CTGTGGACAA CTGACATGGC AGCATCTGTA
    AAGGAATTGA TATTTCATCT CTTGGCTGAA TTGCTTCGCA AAATTCACAA CCTGGAGCAG
    AAGAAAAGTC CTGCAGGATT ATCATCTTCT ATTGCCCTTC AGCTGAATCC CTGTCTAGCT
    ATGCTTATGG CCCTGCAGTC AGAACTCCAT AAGCTGTACG ATGAGGAAAC ACAAAACTGG
    GTGTCTGGAA ATGCATGCGG TGGTTCTGGT GTTGGAGCTG CTGACCAAGG AAGATTTTCA
    ACATACTTCC ATGCTCTGAT GGAAGTTTGC CTTGCTGTAG CAGAAGTAAC TCTACCTATC
    AATATGAGCG TAACAGCTAA TGTGATATCT TCAACGAGTG CACCAAATCT TAGTGACTCT
    TCCTCCTCAT CTTCATCTTC TCCAGGACAG ACACCGCAGA GTCCAAGCTT ATTGTCAAAG
    AGGAAAAAAG TCAAAATGAA GAGAGAAAAG ACATCGTCTT CAGGCAAACG ATCTTCATCC
    AGAGCAGCTG AAACGGATGC TGCAATCCTC AGTATAGGAG GAAGCAAGCC TGAGGATATG
    CTATGGTTTC ATAGAGCACT GACTTTGCTT ATCATTCTCA GACACCTAAC GAAAAAGGAT
    CCGCAAGGGT TGGGTGTGAC AAACGATGCT GTAGCAGATG CATGTCAGGC ACTGGTAGGA
    CCCACAGCTC ATAGCCGTTT GCTTGTCATT TCTGGAATAC CAACTCACTT GGAGGAGAGT
    ACTGTCAGAA ATGCAATCAG GAAGGCGTGT AATGCGCATG GTGGAGTCTT CAAGGATGAA
    ATCTACATCC CTCTGCAGGA AGAGGATCCC AAAAAAATCA AGGACAAAGC AGAAGGAGGA
    GAATGCAGAA CTGAGCTGGA AAAGCCAACT GTTTCAAGTA GTGCGGACAG TCTGGATATC
    AGCAGCTCTA GCAGTGTCAC ACCTGCTATG AGCGTTAGTG CGTCAGCATC AACGAGCCAG
    GCTTCCCTGT GCAGCTCACA GGGTATCTCC CGGACTGTTA GTGACATCTC TGTTGATCAG
    TACCAGGCTG GGCTGGAACT GGCCATCCCA CCAGGACTGC TGGAGCCACA CGTTTCCAGC
    CAGGAGAGCT TGGATCTGTC GCACTGCAGT ACTGGGAGCC TTGGCAGCCT TGGCAGCCTC
    GGGGAGCCGC TTGACAATGT AGAGACAGCA TCAGTGTCTG ATGTCGGGTC GATGTACACG
    GTCACATCTC TGGACAACCA GCAACTTCTG TCACGGCCTA TCAAAGGTTT TGCTGTGGTG
    GAGATACGAT CAAGAGCTAA AATAGAGAAA ATCCGAGCCA GCCTATTTAA CAGTAGTGAT
    CTCATTGGTT TGTCAAGTCT GGATGGTGAA GACGAACTGA TGGAAATGTC AACTGAAGAG
    ATTTTAACTG TTTCTACTGT AAATCAGAGT TTATTTGACA CACAAGGGAG CCCGGCGCTA
    GAAGACTACT TCAATGATAA GTCAATAAAA GGTGAGAAAT TGGTTCCTGG TGCTCGGGAG
    GTCCTAACAG AAATATTTAA AAGCTGTGTC CATTCTGAGC AAATGCTGAG CCTGACTCCA
    ACAAAACCAA TCAAGGTTGC TGATATCTAT CTTAGTAAAG AGCAGATAAA TTCTCAGACT
    CCTGGAAACC TTCTTCATGT CTTCTTCACA AATGTCCGTC CTCCAAAGAA GGTGCTGGAG
    GACCAGCTTA CTCAGATTCT AAGGAAATAC GGTGTGCCCA AGCCGAAATT TGACAAGAGC
    AAATACAACA AGGCAGGCAA AGAACAGCAT CCGGTGAAGG TTGTGAGCAC CAAGCGCCCA
    GTTACAAAAC CACCCACAAA AGAAAAGTCT GTACTCAACA GCGTCAGCCG AACTGCTTTG
    AGTGAGAAGA AACCTACTGT AAAGCCAAAG TCTCCTGAGA AGAGCAAACC TGAAGAGAAG
    GACCCTGAAA AGTCTCCCAC AAAGAAACAA GAAGTTCCTG AGGAGAAGTA CCTGACACTG
    GATGGATTTC ACAGATTTGT CATTGACCGA TCTAAGCAGG ATATCCGTAG TGTGTGGAGA
    GCTATTCTGT CCTGTGGATA TGATCTGCAT TTTGAAAGAT GTGCCTGCAT TGATGCAAGA
    CATGCACAGA AAGCATCTCG GAAGTGGACT CTGGAAATGG ACGTGGCTCT CGTTCAGTAC
    ATTAACAGGC TTTGTCGTCA CCTCGCAATT ACACCAGCAC GACTCCATCC ACATGAAGTA
    TATTTGGATC CAGCTGATGC AGCAGATCCT AGAATCTCCT GTCTGTTGAA TGTTCCCGTT
    GAAAGTCTCC GCCTGCGGTT TGCACTGCTT CAGTCTCTCA ATACGACGTT AGAGACGTTT
    TTCTTACCGC TGGTAGAGCT TCGGCAGACT GAGATGTACA GTAACAGCAT TGCTGCGCTG
    CTGCAGGAGG CCAAAGGTTT AATCTTCTAT GACACAAAAA TAACCGTAAT GAACAGAGTG
    CTGAATGCCA CTGTCCAAAG AACTGCTGAT CACGCAGCCC CAGAAATCAC CTTGGATCCC
    TTGGAAATAG TTGGAGGGGA GATCCGTTCT TCAGAAAATT CCTACTTCTG CCAGGCAGCA
    AGGCAGCTGG CCTGTGTGCC ATCCTCACAG CTCTGCGTCA AACTCGCCAG TGGTGGGGAC
    CCTACGTACG CGTTCAACAT CCGGTTCACA GGGGAGGAGG TCCATGGAAC AAGTGGATCT
    TTCCGTCACT TCCTCTGGCA AGTTTGTAAG GAGCTGCAGA GCTCCTCGCT CTCTCTTCTC
    CTGTTGTGTC CAAGCTCTGC GGTCAACAAG AACAAGGGGA AGTACATTTT GACACCTAGC
    CCCATAACCT ATGCAGAGGA GCAGCTGTTC CATTTCTTTG GGCAGCTTCT GGGCATTGCA
    ATTCGAGCAG ATGTTCCCCT GCCACTCGAC CTCCTGCCCT CATTCTGGAA GACTCTGGTA
    GGAGAGCCGC TGGATCCTGA TGTTGATCTC CAGGAAGCTG ATATCCTCAC CTACAACTAC
    ATTAAAAAGT TTGAAAATAT AAATGATGAA ACTGAACTGG AAGCTCTGTG TGCAGAAATT
    GCTTCTCAGC ATTTAGCAAC AGAGAGCCCT GACTGTCCTA ATAAGCCATG CTGCAAATTC
    ACCTACCTGA CCATGACAGG GGAAGAGGTG GAACTCTGTC CCAGGGGCAG GCACGTCCCA
    GTGGGATGGG AGAACAAGGA CGTGTATGCA GCTGCAATCA GGAGCTTGAG AATGCGTGAG
    CTGCAGACTC CAGAGTGCAT GACTGCAGTG CGTGCTGGGC TTGGGTCCAT CATTCCCCTG
    CAGCTTCTGA CCACGCTGAC CCCTCTTGAG ATGGAGCTGA GGACATGTGG GCTTCCATAC
    ATCAACCTGG AGTTTCTCAA GGCGCACACG ATGTACCAGG TGGGACTGAT GGAAACTGAT
    CAGCACATTG AGTTTTTTTG GAGCGCGCTG GAGATGTTCA CTCAGGAGGA GCTCTGCAAG
    TTCATAAAGT TTGCCTGTAA CCAGGAGCGG ATCCCCTTCA CGTGTCCTTG CAAGGACGGC
    GGTCCCGATA CCGCCCACGT CCCGCCTTAC CCCATGAAAA TAGCCCCCCC TGATGGAGCT
    GCAGGTTCTC CGGACTCTCG GTACATCCGT GTGGAAACGT GCATGTTCAT GATCAAACTT
    CCTCAGTACT CATCACTGGA CATCATGCTG GAGAAGCTCC GATACGCCAT CCACTACCGG
    GAGGATCCTC TCAGCGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42926
    Clone ID Related Accession (Same CDS sequence) XM_015275542.1
    Accession Version XM_015275542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
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    1321
    1381
    1441
    1501
    1561
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    1801
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    1981
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    11521
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    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TTGGTGATCT ATGCTGGTCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTAGTAAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGGA GTGGTCGTGA GCACAACACT CCAGACAAAG CCATGTGGGG CTTTGCTTGC
    ACTGTTCGAG CACAGGAGTC TTCAGAGGAT GTGTCAGGAG GCCTGCCATT CTTAGTTGAC
    CTGGCACTTG GCCTTTCTGT GTTAGCTTGT TCTATGCTTC GGATTTTATA CAATGGACCA
    GAAATTACCA AAGATGAAGA AACCTGTCAA GAGCTTCTAA GATCTAAACT ATTACAAAGG
    TGCCAATGGC AGGTGGAAGC TAATGGTGTA ATATCTCCAG CCCTTACACC GAGTCCTTCT
    CCACTGCCTC TTACTATAGA TGAGGACAGA GAATTTACTT ATCCTTCTGA TGTGCTGGTG
    CCTCCCATTG GACACTATTT TGATCTGCCT AGGATTAGGC TGCCTCCAGG AATCATGATA
    AAACTCAGGG AAATTTCTGG ACGTGCTCGG CCTCAATTTA GACCTAGCAT AAAGGAAGTG
    ATTCAGCCCG AGGTAATGGA GGAGATGGTA GTTTCATGTG TGATTAAACA TTTGAATTTG
    GTTGATGCAC TCCAGTCCCT GATAAACTTC CAGTATCAGG AAGAACATGC TGAAGAATAT
    GATTTACTTT GTAAAATTAT GGGAGAGACT TTTAAGAAGC TAAATGCCAT GGAGAGACAA
    TTACAGAGTG TGGCTGAGCT GGAACAGAAA TGGCAGAGTG AGGTTGATGA CGCCATGCAT
    GGGAAGCTGG AGAATAATGT TCCCTTCTTC TATGATTACC ACTTCAATGA GAGCAAGATG
    AAAGAACTGG AACTTCTGTG TTCTATGAAG GAAGTTTCTT TTGATGGAAG TGATCTTGAG
    AATGTGGTCC TGGCATTAAG GGAGAAGTTC TTTCAAGAGG TGAATTCACT GATACAGAAG
    ACTTATCATC CCCTAGCGAA AACAAAAGCA TTAGTTAAAA GCTTGATGAA CAGGGCAGAG
    CTGTTGCTGC ATGTCACTAT TGCAGCGCAG TCTGGTATCA CAAGAAGTAT ATCTGGAACC
    CCTGCAGAGA CTCCAGCCTG TAAGTCTGCA TCTGAAACAA AAGTGATATC TCATGCAGTG
    CGCCAGCCCG TCTTCCTTCG CAGTATGTCA GCACCATCTG ACTTAGAGAT GATTGGTAAC
    GAAGATATTG AGTTTGCTCG ATCAAGTCAA AGGCGACGCC ATGTTACCAG CCACAGAAGT
    AGCTCTTTCA CTCTTCTGCA ATCACTGACC ATTGAGGACA GCAGAGATAA ACCTACATAC
    AGTGTCCTAC TGGGGCAGCT CTTTGCTTTC ATTGGAACTA ATCCTGACCA AGCGGTATCC
    AGCAGCAGTT TTCTGCTCGC TGCTCAGACA AGGTGGCGGC GTGGGAACAC AAGGAAGCAA
    GCCTTAGTGC ATATGAGAGA GCTGCTGACT GCGGCTGTTC GGGTTGGTGG GGTGACACAC
    CTCGTGGGCC CTGTGACCAT GGTACTTCAG GGAGGACCAA GAATTGAAGA GCTCACGTGT
    GGTGGGATGG TGGAACAAGT CCAGGAAGCT TTTGGGGAGA CTATGACATC CGTGGTCTCG
    CTGTGTGCTC GCTACCCCAT TGCTTGTGCA AACAGCATTG GCCTCTTATG CACTATACCT
    TACACAAGGA GTGAAGAGAA GTGTTTGGTA CGTAGTGGCC TGGTACAGCT TATGGACAGG
    CTGTGCAGCT TAAGCAGCCA GGCAGAATCC AGTTCAAATG AAAAACAGAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCTTG GGCTGCCTTT CAGGTGCTGG CCAATCGCTG TGTTGAATGG
    GAGAAAGAAG AAGGTGGTTC AACAGAAGCT GTTCACTCTG GTCTGGCTCG TCAGGTCTCC
    AGCCTTCTTA CCAATCACCT TGCACGAGCT ACAGAGTGTT GTGGTAATCA AGCTGCAGGA
    AATGATGCAC TTCAAGACGT CCTCAGTCTT CTTAATGACC TGTCGAGGAG TCACATAGGT
    AAAGCAATCC TGAGCCAGCC AGCCTGTGTG TCCAAACTTC TGTCACTTCT CTTGGATCAA
    CGTCCCTCTC CAAAGTTGGT TCTTATAATC CTTCAGTTGT GTCGTGCTGC TCTACCTCTC
    ATGAGTGTTG AAGACTGTGG AAATGTAGAA CTGCCTCCCT GGAGTTACTC TGTGCCCTCC
    CTGAACAGTG AACAAGATGA TCCCAGCGAC CCAGCCTCCA AGATTGCATC TCTGCTCTTA
    GCAAAGCTGG CAGATTATGT AGTACCAGGA TGCCAGACAG TTCTTTCTCC AACTGCTTCT
    GAACCAGATA CTGCTTTAGC AAAAAGCAGC CCTAAGAATT CTATAAAAGG CGATAAAGAC
    CCTGGAGAAG AAAGTGAGGC AGTGGATGGC AAACTTTCTA TTTTCATCCA TAAACGGGAA
    GACCAGTCAT CCCACGAAGT GCTTCAGCCA TTGCTGAGTA GCTCCGAAGG TCGCCCATTC
    CGACTAGGAA CTGGAGCCAA CATGGAGAAG GTGGTGAAAA TGGATCGTGA CATGACTAAA
    AGTGGCTGCT GTGAAGTTAT CACAGAAGAA GCTGCTGCAG CTCTTCGCAA GGCAACTAAG
    TGGGCTCAGT CTGGTCTGAT AGTTAGTGTT GGGCCACCCA TAGAAACTGT CAATCCAGAA
    GCAACCAGTG GGATGTCCTC TGGGGACAAA AAGAAGACTG CACAGACATC TATTTGCAGA
    GAGAGAAATT CTGAGCTTGC CAGGACTGAT CCTGTAAGAC CATTCATCAG TGGTCATGTT
    GCAAATAGCA TGGCAGCAGA AGTGATCGCT TTGCTACATA GCTTGTTGAT GGCACCAGAA
    TCAAATGCGG CTCAAATATG GACAACAACT GCTGAAAAAG TTCTGTCTCG GGCTCTGATG
    TACATTCCAC AACTCGGGAA ATACGCAGAG AGTATTTTGG AGAATGGAAG CAGCAGTGGA
    AGGAAGCTTG CTAAACTTCA AAGAATTGCT CGACAGGCAG TAGCTGCTCT TTGTGCTCTA
    GGTGGCTTTA AGGAGACAAT TAAAATCGGT TCTGAAGTTC AGGTTTTAGG TAAAGGAATA
    GCAGGAAGCA TTGGAGTTGT GGCATCTATT AATGAACAAG AAGGTATAGC AACGGTCAAA
    TTTCCGCCTA CCACTATAGA CAGTAGAAAG ACCTCACAAG CATCAGACAC TTTAACTATT
    CCGTTATCTA GGCTCTGTGT TCCAAGATCT GAGGCATTGC CTCTTCATAA ACTGTCCATT
    ACTGAGAAGG TAGTACAGGC AGTCCAGTCC ATGTTGCTTC CTCAGGAGGG AAGTCTATCT
    ATTCACACCT CACTTCCTGC AACAGGAGAT GGCTCAACTC CAGTAATGGC AGTGGTCCGG
    CTCCTTGCTG AAATAAGAAC CAGAGCATGC CTGGTGATGG CTCAGCTGTT AGAAGACAGT
    TCATTCTGTG AAGAATTCAT TCAACAGTGC CCAGCTGCTG TAGAGGTTCT TAATTTGGTA
    GCCCAGGAGT GCAGCCCTGG AGAGAGGCTC ACTGTAGTAG AAATGCAGTG TGAAAGGTTA
    AGGATGTTGT ATCGTGACTG TGCTCGACCC CCACCTCCTC CACTGCAGGC AGACAGAAGG
    CAGCCCAAAG AAATCACTTG GAGCCCATCA AGAGTGTTTC CCCCAGTACG AGCATGCATG
    TTTTCATCAC ATCTTGCTGC TGTGACCTTC CTGGCTGATC CAAGTGCAGG TGGGGGACTT
    CCTCGGGGAA CGTTCATTTA TGCCACTTCT CCAGTTCCCG TGCAGGCACC ATCATTCTAC
    TGGGAAATTG AAATAGTTTC CTATGGAGAT ACAGATGATG ACACTGGTCC AATAGTTTCA
    TTTGGATTCG CTACAGAGGC AGAAAAACGG GATGGGGCTT GGACAAACCC AGTGGGCACC
    TGTCTTTTTC ATAACAATGG GCGAGCAGTG CATTACAATG GCTCCAGCTT GTTACAGTGG
    AAGAGTGTTC GATTGGATGT GACTCTTTCT CCTGGTGATG TGGCTGGAAT TGGATGGGAA
    AGAGCAGAAG GAACTCCACC CCCACCAGGG CAGCCTGCTA AAGGCAGAGT TTATTTTACT
    TATTGCGGGC AGCGACTTGG GCCATACCTA GAGGATGTCT CTGGTGGAAT GTGGCCAATT
    GTTCACATAC AGAAAAAGAA CACAAAAGTC CGTGCTAATT TTGGATCTCG CCAGTTTGCC
    TATGCCGAAG GCCAAGCTCA CAGGAATGCT GCTGATCTGT GTATTGACCT AGCTGAAGAG
    ATAAGTGCCA ACTTTGAGGC ATTGCCATTT GCTATGGCTT CTGATAGCGA TAACGACGCT
    GGCACCAGCA TAGCTTCTGA TCCTGGGACT CACGGACCTC CCTGTAGAAT TGCTGCTGTT
    GCTACGGCTC AACAACAATA TGACAGTGAC ACATCTTGTC ACTATAAAAT GGAACTAAGC
    TATGAGAACT TCATCACATC AGGGCCTGAT CCTCATCCCC CTCCTATTGC AGATGATGAA
    AGTGATGATG ACGACGATGA TGATATTCCC AGGGAGGACC ACTATGCCCT GCTGGTTAAA
    GCCTGGGAAA CTAAAGTTTT CCCTACAATT AGAAGAAGAT TCCGTAATGA GGCTGAGAGG
    AAATCTGGGC TGGATCAAAT TAAAGGAGCC TTACAGTTAG GAATGGTTGA TATAGCACGA
    CAAACTGTAG AATTCTTGTA TGAGGAAAAC GGTGGCATCC CGAGAGACCT CTACCTTCCT
    ACCATTGAGG ATATCAAAGA TGAAGCAAAC AAGTTTACAA TCGATAAAGT ACGAAAAGGT
    CTCACTGTTG TAACTCGCTC TCCTGACAGT AACAACGTTA CCAGCAGTGC TGTTGGAACA
    GCTTTACCCA AGTTTGCTAT TCGGGGAATG CTGAAGACAT TTGGTCTTCA TGGTGTTGTC
    CTGGATGTTG ATTCAGTGAA TGAATTGGTA CAAGTAGAGA CCTATCTCAG AAGTGAGGGA
    GTTCTGGTAA GATATTGGTA TCCTATTGAC ATGTTAGAAA GGCCACCTGC TGGTTATAGG
    AGGACTGCAA CAAATGGATT GGTTACCCTG GACAATACCA ATATCCAAAT CCACAGAGAG
    CTTTTGAGAA GTGAAGCTGC ACTGGCCAAG CTGTACTGCC GCATGGCTCT CCTCAATATC
    TTTGCCCCCA AATCTCCACA TATGTTCACC CGTCTGTTTC ATATCCCCGC TATCCGTGAT
    ATCACTCTGG AGCACCTGCA GTTGCTATCC AACCAACTTC TCGCACCACC TCTTCCTGAT
    GGAACAATCA GTTCAAGTTC CATTCTTTTG GCTCAGTCAC TGCAACACTG CATCCATTCC
    CAGAACTGTG CTGCCACAGA CCTCTTCTAC CAGGGCAATT CACAAGCCAT CAGAGAGTGG
    CTTACTGTTG CCATCACTCG GACACTGCAT CAAGGAGAGG AGAGCCTGTT AGACCTCACT
    AAGCAGATCT GCTCTTTTTT GCAGACTGCA CCAGAACAGT TTCCTGCTGA AGAATTCCCA
    ATTTCAGAAT CCAAAGTCAA CATGGATGTT AATTTTCCTG GGGCTGCGTT TGTAATTGTG
    TCCTGTAAAG AAAGCCAGTC TGGATTCCGC AAAGATTCTT CCCTGTATAA AGCTCCTTGG
    GCTCGGGTGC TTGTGTACGG GCTGGGGCAC AAAGTTAAGA GGAATGGCCA GTTAAACCTG
    ATTGAGGCAG TGTGTTACCC TCGAGACGCC TCTCCGACCA ACACAGGCCT AACACCTCCC
    CCAACAACCA ATCAGTACCC TTCAGTGATC ACTCCTACAG ACAAAGTGCA CATCAAACTA
    GGAGTGTCTC CTCCTCCTGG AGCTGTGCTG GTCTTGCATT CTCTTCCCCT TGAGTTCCCC
    CTGGCAATGG CATTTACAGA ACAGCTGTTA ACTTGGAAAC TGGAAGATGT GGATGGGAAA
    TCAGAAGATG AACTGGATAC TATTCCTCCT TCAGTTCTTT TGCAAGTAGT GGAATTTTTA
    GGGAACTTTC TGTGGACAAC TGACATGGCA GCATCTGTAA AGGAATTGAT ATTTCATCTC
    TTGGCTGAAT TGCTTCGCAA AATTCACAAC CTGGAGCAGA AGAAAAGTCC TGCAGGATTA
    TCATCTTCTA TTGCCCTTCA GCTGAATCCC TGTCTAGCTA TGCTTATGGC CCTGCAGTCA
    GAACTCCATA AGCTGTACGA TGAGGAAACA CAAAACTGGG TGTCTGGAAA TGCATGCGGT
    GGTTCTGGTG TTGGAGCTGC TGACCAAGGA AGATTTTCAA CATACTTCCA TGCTCTGATG
    GAAGTTTGCC TTGCTGTAGC AGAAGTAACT CTACCTATCA ATATGAGCGT AACAGCTAAT
    GTGATATCTT CAACGAGTGC ACCAAATCTT AGTGACTCTT CCTCCTCATC TTCATCTTCT
    CCAGGACAGA CACCGCAGAG TCCAAGCTTA TTGTCAAAGA GGAAAAAAGT CAAAATGAAG
    AGAGAAAAGA CATCGTCTTC AGGCAAACGA TCTTCATCCA GAGCAGCTGA AACGGATGCT
    GCAATCCTCA GTATAGGAGG AAGCAAGCCT GAGGATATGC TATGGTTTCA TAGAGCACTG
    ACTTTGCTTA TCATTCTCAG ACACCTAACG AAAAAGGATC CGCAAGGGTT GGGTGTGACA
    AACGATGCTG TAGCAGATGC ATGTCAGGCA CTGGTAGGAC CCACAGCTCA TAGCCGTTTG
    CTTGTCATTT CTGGAATACC AACTCACTTG GAGGAGAGTA CTGTCAGAAA TGCAATCAGG
    AAGGCGTGTA ATGCGCATGG TGGAGTCTTC AAGGATGAAA TCTACATCCC TCTGCAGGAA
    GAGGATCCCA AAAAAATCAA GGACAAAGCA GAAGGAGGAG AATGCAGAAC TGAGCTGGAA
    AAGCCAACTG TTTCAAGTAG TGCGGACAGT CTGGATATCA GCAGCTCTAG CAGTGTCACA
    CCTGCTATGA GCGTTAGTGC GTCAGCATCA ACGAGCCAGG CTTCCCTGTG CAGCTCACAG
    GGTATCTCCC GGACTGTTAG TGACATCTCT GTTGATCAGT ACCAGGCTGG GCTGGAACTG
    GCCATCCCAC CAGGACTGCT GGAGCCACAC GTTTCCAGCC AGGAGAGCTT GGATCTGTCG
    CACTGCAGTA CTGGGAGCCT TGGCAGCCTT GGCAGCCTCG GGGAGCCGCT TGACAATGTA
    GAGACAGCAT CAGTGTCTGA TGTCGGGTCG ATGTACACGG TCACATCTCT GGACAACCAG
    CAACTTCTGT CACGGCCTAT CAAAGGTTTT GCTGTGGTGG AGATACGATC AAGAGCTAAA
    ATAGAGAAAA TCCGAGCCAG CCTATTTAAC AGTAGTGATC TCATTGGTTT GTCAAGTCTG
    GATGGTGAAG ACGAACTGAT GGAAATGTCA ACTGAAGAGA TTTTAACTGT TTCTACTGTA
    AATCAGAGTT TATTTGACAC ACAAGGGAGC CCGGCGCTAG AAGACTACTT CAATGATAAG
    TCAATAAAAG GTGAGAAATT GGTTCCTGGT GCTCGGGAGG TCCTAACAGA AATATTTAAA
    AGCTGTGTCC ATTCTGAGCA AATGCTGAGC CTGACTCCAA CAAAACCAAT CAAGGTTGCT
    GATATCTATC TTAGTAAAGA GCAGATAAAT TCTCAGACTC CTGGAAACCT TCTTCATGTC
    TTCTTCACAA ATGTCCGTCC TCCAAAGAAG GTGCTGGAGG ACCAGCTTAC TCAGATTCTA
    AGGAAATACG GTGTGCCCAA GCCGAAATTT GACAAGAGCA AATACAACAA GGCAGGCAAA
    GAACAGCATC CGGTGAAGGT TGTGAGCACC AAGCGCCCAG TTACAAAACC ACCCACAAAA
    GAAAAGTCTG TACTCAACAG CGTCAGCCGA ACTGCTTTGA GTGAGAAGAA ACCTACTGTA
    AAGCCAAAGT CTCCTGAGAA GAGCAAACCT GAAGAGAAGG ACCCTGAAAA GTCTCCCACA
    AAGAAACAAG AAGTTCCTGA GGAGAAGTAC CTGACACTGG ATGGATTTCA CAGATTTGTC
    ATTGACCGAT CTAAGCAGGA TATCCGTAGT GTGTGGAGAG CTATTCTGTC CTGTGGATAT
    GATCTGCATT TTGAAAGATG TGCCTGCATT GATGCAAGAC ATGCACAGAA AGCATCTCGG
    AAGTGGACTC TGGAAATGGA CGTGGCTCTC GTTCAGTACA TTAACAGGCT TTGTCGTCAC
    CTCGCAATTA CACCAGCACG ACTCCATCCA CATGAAGTAT ATTTGGATCC AGCTGATGCA
    GCAGATCCTA GAATCTCCTG TCTGTTGAAT GTTCCCGTTG AAAGTCTCCG CCTGCGGTTT
    GCACTGCTTC AGTCTCTCAA TACGACGTTA GAGACGTTTT TCTTACCGCT GGTAGAGCTT
    CGGCAGACTG AGATGTACAG TAACAGCATT GCTGCGCTGC TGCAGGAGGC CAAAGGTTTA
    ATCTTCTATG ACACAAAAAT AACCGTAATG AACAGAGTGC TGAATGCCAC TGTCCAAAGA
    ACTGCTGATC ACGCAGCCCC AGAAATCACC TTGGATCCCT TGGAAATAGT TGGAGGGGAG
    ATCCGTTCTT CAGAAAATTC CTACTTCTGC CAGGCAGCAA GGCAGCTGGC CTGTGTGCCA
    TCCTCACAGC TCTGCGTCAA ACTCGCCAGT GGTGGGGACC CTACGTACGC GTTCAACATC
    CGGTTCACAG GGGAGGAGGT CCATGGAACA AGTGGATCTT TCCGTCACTT CCTCTGGCAA
    GTTTGTAAGG AGCTGCAGAG CTCCTCGCTC TCTCTTCTCC TGTTGTGTCC AAGCTCTGCG
    GTCAACAAGA ACAAGGGGAA GTACATTTTG ACACCTAGCC CCATAACCTA TGCAGAGGAG
    CAGCTGTTCC ATTTCTTTGG GCAGCTTCTG GGCATTGCAA TTCGAGCAGA TGTTCCCCTG
    CCACTCGACC TCCTGCCCTC ATTCTGGAAG ACTCTGGTAG GAGAGCCGCT GGATCCTGAT
    GTTGATCTCC AGGAAGCTGA TATCCTCACC TACAACTACA TTAAAAAGTT TGAAAATATA
    AATGATGAAA CTGAACTGGA AGCTCTGTGT GCAGAAATTG CTTCTCAGCA TTTAGCAACA
    GAGAGCCCTG ACTGTCCTAA TAAGCCATGC TGCAAATTCA CCTACCTGAC CATGACAGGG
    GAAGAGGTGG AACTCTGTCC CAGGGGCAGG CACGTCCCAG TGGGATGGGA GAACAAGGAC
    GTGTATGCAG CTGCAATCAG GAGCTTGAGA ATGCGTGAGC TGCAGACTCC AGAGTGCATG
    ACTGCAGTGC GTGCTGGGCT TGGGTCCATC ATTCCCCTGC AGCTTCTGAC CACGCTGACC
    CCTCTTGAGA TGGAGCTGAG GACATGTGGG CTTCCATACA TCAACCTGGA GTTTCTCAAG
    GCGCACACGA TGTACCAGGT GGGACTGATG GAAACTGATC AGCACATTGA GTTTTTTTGG
    AGCGCGCTGG AGATGTTCAC TCAGGAGGAG CTCTGCAAGT TCATAAAGTT TGCCTGTAAC
    CAGGAGCGGA TCCCCTTCAC GTGTCCTTGC AAGGACGGCG GTCCCGATAC CGCCCACGTC
    CCGCCTTACC CCATGAAAAT AGCCCCCCCT GATGGAGCTG CAGGTTCTCC GGACTCTCGG
    TACATCCGTG TGGAAACGTG CATGTTCATG ATCAAACTTC CTCAGTACTC ATCACTGGAC
    ATCATGCTGG AGAAGCTCCG ATACGCCATC CACTACCGGG AGGATCCTCT CAGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131028.1
    CDS263..13522
    Translation

    Target ORF information:

    RefSeq Version XM_015275542.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TTGGTGATCT ATGCTGGTCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTAGTAAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGGA GTGGTCGTGA GCACAACACT CCAGACAAAG CCATGTGGGG CTTTGCTTGC
    ACTGTTCGAG CACAGGAGTC TTCAGAGGAT GTGTCAGGAG GCCTGCCATT CTTAGTTGAC
    CTGGCACTTG GCCTTTCTGT GTTAGCTTGT TCTATGCTTC GGATTTTATA CAATGGACCA
    GAAATTACCA AAGATGAAGA AACCTGTCAA GAGCTTCTAA GATCTAAACT ATTACAAAGG
    TGCCAATGGC AGGTGGAAGC TAATGGTGTA ATATCTCCAG CCCTTACACC GAGTCCTTCT
    CCACTGCCTC TTACTATAGA TGAGGACAGA GAATTTACTT ATCCTTCTGA TGTGCTGGTG
    CCTCCCATTG GACACTATTT TGATCTGCCT AGGATTAGGC TGCCTCCAGG AATCATGATA
    AAACTCAGGG AAATTTCTGG ACGTGCTCGG CCTCAATTTA GACCTAGCAT AAAGGAAGTG
    ATTCAGCCCG AGGTAATGGA GGAGATGGTA GTTTCATGTG TGATTAAACA TTTGAATTTG
    GTTGATGCAC TCCAGTCCCT GATAAACTTC CAGTATCAGG AAGAACATGC TGAAGAATAT
    GATTTACTTT GTAAAATTAT GGGAGAGACT TTTAAGAAGC TAAATGCCAT GGAGAGACAA
    TTACAGAGTG TGGCTGAGCT GGAACAGAAA TGGCAGAGTG AGGTTGATGA CGCCATGCAT
    GGGAAGCTGG AGAATAATGT TCCCTTCTTC TATGATTACC ACTTCAATGA GAGCAAGATG
    AAAGAACTGG AACTTCTGTG TTCTATGAAG GAAGTTTCTT TTGATGGAAG TGATCTTGAG
    AATGTGGTCC TGGCATTAAG GGAGAAGTTC TTTCAAGAGG TGAATTCACT GATACAGAAG
    ACTTATCATC CCCTAGCGAA AACAAAAGCA TTAGTTAAAA GCTTGATGAA CAGGGCAGAG
    CTGTTGCTGC ATGTCACTAT TGCAGCGCAG TCTGGTATCA CAAGAAGTAT ATCTGGAACC
    CCTGCAGAGA CTCCAGCCTG TAAGTCTGCA TCTGAAACAA AAGTGATATC TCATGCAGTG
    CGCCAGCCCG TCTTCCTTCG CAGTATGTCA GCACCATCTG ACTTAGAGAT GATTGGTAAC
    GAAGATATTG AGTTTGCTCG ATCAAGTCAA AGGCGACGCC ATGTTACCAG CCACAGAAGT
    AGCTCTTTCA CTCTTCTGCA ATCACTGACC ATTGAGGACA GCAGAGATAA ACCTACATAC
    AGTGTCCTAC TGGGGCAGCT CTTTGCTTTC ATTGGAACTA ATCCTGACCA AGCGGTATCC
    AGCAGCAGTT TTCTGCTCGC TGCTCAGACA AGGTGGCGGC GTGGGAACAC AAGGAAGCAA
    GCCTTAGTGC ATATGAGAGA GCTGCTGACT GCGGCTGTTC GGGTTGGTGG GGTGACACAC
    CTCGTGGGCC CTGTGACCAT GGTACTTCAG GGAGGACCAA GAATTGAAGA GCTCACGTGT
    GGTGGGATGG TGGAACAAGT CCAGGAAGCT TTTGGGGAGA CTATGACATC CGTGGTCTCG
    CTGTGTGCTC GCTACCCCAT TGCTTGTGCA AACAGCATTG GCCTCTTATG CACTATACCT
    TACACAAGGA GTGAAGAGAA GTGTTTGGTA CGTAGTGGCC TGGTACAGCT TATGGACAGG
    CTGTGCAGCT TAAGCAGCCA GGCAGAATCC AGTTCAAATG AAAAACAGAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCTTG GGCTGCCTTT CAGGTGCTGG CCAATCGCTG TGTTGAATGG
    GAGAAAGAAG AAGGTGGTTC AACAGAAGCT GTTCACTCTG GTCTGGCTCG TCAGGTCTCC
    AGCCTTCTTA CCAATCACCT TGCACGAGCT ACAGAGTGTT GTGGTAATCA AGCTGCAGGA
    AATGATGCAC TTCAAGACGT CCTCAGTCTT CTTAATGACC TGTCGAGGAG TCACATAGGT
    AAAGCAATCC TGAGCCAGCC AGCCTGTGTG TCCAAACTTC TGTCACTTCT CTTGGATCAA
    CGTCCCTCTC CAAAGTTGGT TCTTATAATC CTTCAGTTGT GTCGTGCTGC TCTACCTCTC
    ATGAGTGTTG AAGACTGTGG AAATGTAGAA CTGCCTCCCT GGAGTTACTC TGTGCCCTCC
    CTGAACAGTG AACAAGATGA TCCCAGCGAC CCAGCCTCCA AGATTGCATC TCTGCTCTTA
    GCAAAGCTGG CAGATTATGT AGTACCAGGA TGCCAGACAG TTCTTTCTCC AACTGCTTCT
    GAACCAGATA CTGCTTTAGC AAAAAGCAGC CCTAAGAATT CTATAAAAGG CGATAAAGAC
    CCTGGAGAAG AAAGTGAGGC AGTGGATGGC AAACTTTCTA TTTTCATCCA TAAACGGGAA
    GACCAGTCAT CCCACGAAGT GCTTCAGCCA TTGCTGAGTA GCTCCGAAGG TCGCCCATTC
    CGACTAGGAA CTGGAGCCAA CATGGAGAAG GTGGTGAAAA TGGATCGTGA CATGACTAAA
    AGTGGCTGCT GTGAAGTTAT CACAGAAGAA GCTGCTGCAG CTCTTCGCAA GGCAACTAAG
    TGGGCTCAGT CTGGTCTGAT AGTTAGTGTT GGGCCACCCA TAGAAACTGT CAATCCAGAA
    GCAACCAGTG GGATGTCCTC TGGGGACAAA AAGAAGACTG CACAGACATC TATTTGCAGA
    GAGAGAAATT CTGAGCTTGC CAGGACTGAT CCTGTAAGAC CATTCATCAG TGGTCATGTT
    GCAAATAGCA TGGCAGCAGA AGTGATCGCT TTGCTACATA GCTTGTTGAT GGCACCAGAA
    TCAAATGCGG CTCAAATATG GACAACAACT GCTGAAAAAG TTCTGTCTCG GGCTCTGATG
    TACATTCCAC AACTCGGGAA ATACGCAGAG AGTATTTTGG AGAATGGAAG CAGCAGTGGA
    AGGAAGCTTG CTAAACTTCA AAGAATTGCT CGACAGGCAG TAGCTGCTCT TTGTGCTCTA
    GGTGGCTTTA AGGAGACAAT TAAAATCGGT TCTGAAGTTC AGGTTTTAGG TAAAGGAATA
    GCAGGAAGCA TTGGAGTTGT GGCATCTATT AATGAACAAG AAGGTATAGC AACGGTCAAA
    TTTCCGCCTA CCACTATAGA CAGTAGAAAG ACCTCACAAG CATCAGACAC TTTAACTATT
    CCGTTATCTA GGCTCTGTGT TCCAAGATCT GAGGCATTGC CTCTTCATAA ACTGTCCATT
    ACTGAGAAGG TAGTACAGGC AGTCCAGTCC ATGTTGCTTC CTCAGGAGGG AAGTCTATCT
    ATTCACACCT CACTTCCTGC AACAGGAGAT GGCTCAACTC CAGTAATGGC AGTGGTCCGG
    CTCCTTGCTG AAATAAGAAC CAGAGCATGC CTGGTGATGG CTCAGCTGTT AGAAGACAGT
    TCATTCTGTG AAGAATTCAT TCAACAGTGC CCAGCTGCTG TAGAGGTTCT TAATTTGGTA
    GCCCAGGAGT GCAGCCCTGG AGAGAGGCTC ACTGTAGTAG AAATGCAGTG TGAAAGGTTA
    AGGATGTTGT ATCGTGACTG TGCTCGACCC CCACCTCCTC CACTGCAGGC AGACAGAAGG
    CAGCCCAAAG AAATCACTTG GAGCCCATCA AGAGTGTTTC CCCCAGTACG AGCATGCATG
    TTTTCATCAC ATCTTGCTGC TGTGACCTTC CTGGCTGATC CAAGTGCAGG TGGGGGACTT
    CCTCGGGGAA CGTTCATTTA TGCCACTTCT CCAGTTCCCG TGCAGGCACC ATCATTCTAC
    TGGGAAATTG AAATAGTTTC CTATGGAGAT ACAGATGATG ACACTGGTCC AATAGTTTCA
    TTTGGATTCG CTACAGAGGC AGAAAAACGG GATGGGGCTT GGACAAACCC AGTGGGCACC
    TGTCTTTTTC ATAACAATGG GCGAGCAGTG CATTACAATG GCTCCAGCTT GTTACAGTGG
    AAGAGTGTTC GATTGGATGT GACTCTTTCT CCTGGTGATG TGGCTGGAAT TGGATGGGAA
    AGAGCAGAAG GAACTCCACC CCCACCAGGG CAGCCTGCTA AAGGCAGAGT TTATTTTACT
    TATTGCGGGC AGCGACTTGG GCCATACCTA GAGGATGTCT CTGGTGGAAT GTGGCCAATT
    GTTCACATAC AGAAAAAGAA CACAAAAGTC CGTGCTAATT TTGGATCTCG CCAGTTTGCC
    TATGCCGAAG GCCAAGCTCA CAGGAATGCT GCTGATCTGT GTATTGACCT AGCTGAAGAG
    ATAAGTGCCA ACTTTGAGGC ATTGCCATTT GCTATGGCTT CTGATAGCGA TAACGACGCT
    GGCACCAGCA TAGCTTCTGA TCCTGGGACT CACGGACCTC CCTGTAGAAT TGCTGCTGTT
    GCTACGGCTC AACAACAATA TGACAGTGAC ACATCTTGTC ACTATAAAAT GGAACTAAGC
    TATGAGAACT TCATCACATC AGGGCCTGAT CCTCATCCCC CTCCTATTGC AGATGATGAA
    AGTGATGATG ACGACGATGA TGATATTCCC AGGGAGGACC ACTATGCCCT GCTGGTTAAA
    GCCTGGGAAA CTAAAGTTTT CCCTACAATT AGAAGAAGAT TCCGTAATGA GGCTGAGAGG
    AAATCTGGGC TGGATCAAAT TAAAGGAGCC TTACAGTTAG GAATGGTTGA TATAGCACGA
    CAAACTGTAG AATTCTTGTA TGAGGAAAAC GGTGGCATCC CGAGAGACCT CTACCTTCCT
    ACCATTGAGG ATATCAAAGA TGAAGCAAAC AAGTTTACAA TCGATAAAGT ACGAAAAGGT
    CTCACTGTTG TAACTCGCTC TCCTGACAGT AACAACGTTA CCAGCAGTGC TGTTGGAACA
    GCTTTACCCA AGTTTGCTAT TCGGGGAATG CTGAAGACAT TTGGTCTTCA TGGTGTTGTC
    CTGGATGTTG ATTCAGTGAA TGAATTGGTA CAAGTAGAGA CCTATCTCAG AAGTGAGGGA
    GTTCTGGTAA GATATTGGTA TCCTATTGAC ATGTTAGAAA GGCCACCTGC TGGTTATAGG
    AGGACTGCAA CAAATGGATT GGTTACCCTG GACAATACCA ATATCCAAAT CCACAGAGAG
    CTTTTGAGAA GTGAAGCTGC ACTGGCCAAG CTGTACTGCC GCATGGCTCT CCTCAATATC
    TTTGCCCCCA AATCTCCACA TATGTTCACC CGTCTGTTTC ATATCCCCGC TATCCGTGAT
    ATCACTCTGG AGCACCTGCA GTTGCTATCC AACCAACTTC TCGCACCACC TCTTCCTGAT
    GGAACAATCA GTTCAAGTTC CATTCTTTTG GCTCAGTCAC TGCAACACTG CATCCATTCC
    CAGAACTGTG CTGCCACAGA CCTCTTCTAC CAGGGCAATT CACAAGCCAT CAGAGAGTGG
    CTTACTGTTG CCATCACTCG GACACTGCAT CAAGGAGAGG AGAGCCTGTT AGACCTCACT
    AAGCAGATCT GCTCTTTTTT GCAGACTGCA CCAGAACAGT TTCCTGCTGA AGAATTCCCA
    ATTTCAGAAT CCAAAGTCAA CATGGATGTT AATTTTCCTG GGGCTGCGTT TGTAATTGTG
    TCCTGTAAAG AAAGCCAGTC TGGATTCCGC AAAGATTCTT CCCTGTATAA AGCTCCTTGG
    GCTCGGGTGC TTGTGTACGG GCTGGGGCAC AAAGTTAAGA GGAATGGCCA GTTAAACCTG
    ATTGAGGCAG TGTGTTACCC TCGAGACGCC TCTCCGACCA ACACAGGCCT AACACCTCCC
    CCAACAACCA ATCAGTACCC TTCAGTGATC ACTCCTACAG ACAAAGTGCA CATCAAACTA
    GGAGTGTCTC CTCCTCCTGG AGCTGTGCTG GTCTTGCATT CTCTTCCCCT TGAGTTCCCC
    CTGGCAATGG CATTTACAGA ACAGCTGTTA ACTTGGAAAC TGGAAGATGT GGATGGGAAA
    TCAGAAGATG AACTGGATAC TATTCCTCCT TCAGTTCTTT TGCAAGTAGT GGAATTTTTA
    GGGAACTTTC TGTGGACAAC TGACATGGCA GCATCTGTAA AGGAATTGAT ATTTCATCTC
    TTGGCTGAAT TGCTTCGCAA AATTCACAAC CTGGAGCAGA AGAAAAGTCC TGCAGGATTA
    TCATCTTCTA TTGCCCTTCA GCTGAATCCC TGTCTAGCTA TGCTTATGGC CCTGCAGTCA
    GAACTCCATA AGCTGTACGA TGAGGAAACA CAAAACTGGG TGTCTGGAAA TGCATGCGGT
    GGTTCTGGTG TTGGAGCTGC TGACCAAGGA AGATTTTCAA CATACTTCCA TGCTCTGATG
    GAAGTTTGCC TTGCTGTAGC AGAAGTAACT CTACCTATCA ATATGAGCGT AACAGCTAAT
    GTGATATCTT CAACGAGTGC ACCAAATCTT AGTGACTCTT CCTCCTCATC TTCATCTTCT
    CCAGGACAGA CACCGCAGAG TCCAAGCTTA TTGTCAAAGA GGAAAAAAGT CAAAATGAAG
    AGAGAAAAGA CATCGTCTTC AGGCAAACGA TCTTCATCCA GAGCAGCTGA AACGGATGCT
    GCAATCCTCA GTATAGGAGG AAGCAAGCCT GAGGATATGC TATGGTTTCA TAGAGCACTG
    ACTTTGCTTA TCATTCTCAG ACACCTAACG AAAAAGGATC CGCAAGGGTT GGGTGTGACA
    AACGATGCTG TAGCAGATGC ATGTCAGGCA CTGGTAGGAC CCACAGCTCA TAGCCGTTTG
    CTTGTCATTT CTGGAATACC AACTCACTTG GAGGAGAGTA CTGTCAGAAA TGCAATCAGG
    AAGGCGTGTA ATGCGCATGG TGGAGTCTTC AAGGATGAAA TCTACATCCC TCTGCAGGAA
    GAGGATCCCA AAAAAATCAA GGACAAAGCA GAAGGAGGAG AATGCAGAAC TGAGCTGGAA
    AAGCCAACTG TTTCAAGTAG TGCGGACAGT CTGGATATCA GCAGCTCTAG CAGTGTCACA
    CCTGCTATGA GCGTTAGTGC GTCAGCATCA ACGAGCCAGG CTTCCCTGTG CAGCTCACAG
    GGTATCTCCC GGACTGTTAG TGACATCTCT GTTGATCAGT ACCAGGCTGG GCTGGAACTG
    GCCATCCCAC CAGGACTGCT GGAGCCACAC GTTTCCAGCC AGGAGAGCTT GGATCTGTCG
    CACTGCAGTA CTGGGAGCCT TGGCAGCCTT GGCAGCCTCG GGGAGCCGCT TGACAATGTA
    GAGACAGCAT CAGTGTCTGA TGTCGGGTCG ATGTACACGG TCACATCTCT GGACAACCAG
    CAACTTCTGT CACGGCCTAT CAAAGGTTTT GCTGTGGTGG AGATACGATC AAGAGCTAAA
    ATAGAGAAAA TCCGAGCCAG CCTATTTAAC AGTAGTGATC TCATTGGTTT GTCAAGTCTG
    GATGGTGAAG ACGAACTGAT GGAAATGTCA ACTGAAGAGA TTTTAACTGT TTCTACTGTA
    AATCAGAGTT TATTTGACAC ACAAGGGAGC CCGGCGCTAG AAGACTACTT CAATGATAAG
    TCAATAAAAG GTGAGAAATT GGTTCCTGGT GCTCGGGAGG TCCTAACAGA AATATTTAAA
    AGCTGTGTCC ATTCTGAGCA AATGCTGAGC CTGACTCCAA CAAAACCAAT CAAGGTTGCT
    GATATCTATC TTAGTAAAGA GCAGATAAAT TCTCAGACTC CTGGAAACCT TCTTCATGTC
    TTCTTCACAA ATGTCCGTCC TCCAAAGAAG GTGCTGGAGG ACCAGCTTAC TCAGATTCTA
    AGGAAATACG GTGTGCCCAA GCCGAAATTT GACAAGAGCA AATACAACAA GGCAGGCAAA
    GAACAGCATC CGGTGAAGGT TGTGAGCACC AAGCGCCCAG TTACAAAACC ACCCACAAAA
    GAAAAGTCTG TACTCAACAG CGTCAGCCGA ACTGCTTTGA GTGAGAAGAA ACCTACTGTA
    AAGCCAAAGT CTCCTGAGAA GAGCAAACCT GAAGAGAAGG ACCCTGAAAA GTCTCCCACA
    AAGAAACAAG AAGTTCCTGA GGAGAAGTAC CTGACACTGG ATGGATTTCA CAGATTTGTC
    ATTGACCGAT CTAAGCAGGA TATCCGTAGT GTGTGGAGAG CTATTCTGTC CTGTGGATAT
    GATCTGCATT TTGAAAGATG TGCCTGCATT GATGCAAGAC ATGCACAGAA AGCATCTCGG
    AAGTGGACTC TGGAAATGGA CGTGGCTCTC GTTCAGTACA TTAACAGGCT TTGTCGTCAC
    CTCGCAATTA CACCAGCACG ACTCCATCCA CATGAAGTAT ATTTGGATCC AGCTGATGCA
    GCAGATCCTA GAATCTCCTG TCTGTTGAAT GTTCCCGTTG AAAGTCTCCG CCTGCGGTTT
    GCACTGCTTC AGTCTCTCAA TACGACGTTA GAGACGTTTT TCTTACCGCT GGTAGAGCTT
    CGGCAGACTG AGATGTACAG TAACAGCATT GCTGCGCTGC TGCAGGAGGC CAAAGGTTTA
    ATCTTCTATG ACACAAAAAT AACCGTAATG AACAGAGTGC TGAATGCCAC TGTCCAAAGA
    ACTGCTGATC ACGCAGCCCC AGAAATCACC TTGGATCCCT TGGAAATAGT TGGAGGGGAG
    ATCCGTTCTT CAGAAAATTC CTACTTCTGC CAGGCAGCAA GGCAGCTGGC CTGTGTGCCA
    TCCTCACAGC TCTGCGTCAA ACTCGCCAGT GGTGGGGACC CTACGTACGC GTTCAACATC
    CGGTTCACAG GGGAGGAGGT CCATGGAACA AGTGGATCTT TCCGTCACTT CCTCTGGCAA
    GTTTGTAAGG AGCTGCAGAG CTCCTCGCTC TCTCTTCTCC TGTTGTGTCC AAGCTCTGCG
    GTCAACAAGA ACAAGGGGAA GTACATTTTG ACACCTAGCC CCATAACCTA TGCAGAGGAG
    CAGCTGTTCC ATTTCTTTGG GCAGCTTCTG GGCATTGCAA TTCGAGCAGA TGTTCCCCTG
    CCACTCGACC TCCTGCCCTC ATTCTGGAAG ACTCTGGTAG GAGAGCCGCT GGATCCTGAT
    GTTGATCTCC AGGAAGCTGA TATCCTCACC TACAACTACA TTAAAAAGTT TGAAAATATA
    AATGATGAAA CTGAACTGGA AGCTCTGTGT GCAGAAATTG CTTCTCAGCA TTTAGCAACA
    GAGAGCCCTG ACTGTCCTAA TAAGCCATGC TGCAAATTCA CCTACCTGAC CATGACAGGG
    GAAGAGGTGG AACTCTGTCC CAGGGGCAGG CACGTCCCAG TGGGATGGGA GAACAAGGAC
    GTGTATGCAG CTGCAATCAG GAGCTTGAGA ATGCGTGAGC TGCAGACTCC AGAGTGCATG
    ACTGCAGTGC GTGCTGGGCT TGGGTCCATC ATTCCCCTGC AGCTTCTGAC CACGCTGACC
    CCTCTTGAGA TGGAGCTGAG GACATGTGGG CTTCCATACA TCAACCTGGA GTTTCTCAAG
    GCGCACACGA TGTACCAGGT GGGACTGATG GAAACTGATC AGCACATTGA GTTTTTTTGG
    AGCGCGCTGG AGATGTTCAC TCAGGAGGAG CTCTGCAAGT TCATAAAGTT TGCCTGTAAC
    CAGGAGCGGA TCCCCTTCAC GTGTCCTTGC AAGGACGGCG GTCCCGATAC CGCCCACGTC
    CCGCCTTACC CCATGAAAAT AGCCCCCCCT GATGGAGCTG CAGGTTCTCC GGACTCTCGG
    TACATCCGTG TGGAAACGTG CATGTTCATG ATCAAACTTC CTCAGTACTC ATCACTGGAC
    ATCATGCTGG AGAAGCTCCG ATACGCCATC CACTACCGGG AGGATCCTCT CAGCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42927
    Clone ID Related Accession (Same CDS sequence) XM_015275543.1
    Accession Version XM_015275543.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12822bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    ATGGGGCTTC TGCTGGTTTT TCCCCTCATT CAGTCTCAAA GTAGAACAGA CCCTTCTCTC 
    TGTAACATAA CCGCTGAGGT GCTATTGAAT TGCCTTCGAG ACTGCCAGCC TTTGAGCTTG
    ACTAAGGAGC CAGCTGACTG TCTCAATGGG ATAGAGTCTT TGCTGTGCTC GTGGTTGGAA
    GAAACTTCTG CTTCAGGTCA TCAGATTCCA TATAAGCAAA AGGAAAATGC TGCTGCTGCC
    CTAGTTGCCT TGGCTTGTGC AAGAGGGTCG CTGAAAACCT TTGTTCATAC AGTGCATCTA
    TTACAGAAAC AGACAGATCT GGGAACCTTG CCTGTGGCTG ATGTGTTATA CAGGTTGTTG
    CTTTTGGAGG GAGGTCCTGG CTCACCTTCT TGTTTGCTTG GAGGAAAGCA TATAGTGTCC
    TGGGGCTTTG AAGACATGTT ACCTGCACCT GATGCCAACA GTGGCTCCAG TGGTGAAAAT
    AAAGATGCAG ACTTAGGACG TTGTCTTGCA GCAGATGGCC TCTACCTTTA TACAACAAAT
    TCAGTGGGAA GAGGAATAAG CAAATTAGGA TCTGGATTAC ATGGTACCTT GCGAGGTTTT
    GTCTACTGCC GAAATGAGGA GCTGGAGGCT GGGTGGGTTG CTTTTGGCAA CAGCAAACTT
    CTTCATCGGC CTGTCTCTTT TGATAATAAG CCTCACTCCC TTTTCCAGGT TGTGGATCAG
    CATACCCTTC AGGTGTGTCA AATAATCCCA ATGCCAGTAA ATCACTTCCC AGTTGGCAGT
    ACTATGACCA CCGTGCATCT TTCTTCAGAT GGTACATATT TCTACTGGAT TTGGTCTCCT
    GCAAGCCTCA ATGAGAAAAC ACCAAAAGGC CACTCTGTCT TTATGGATGT TTTTGAACTT
    ATAGTAGAAA ATGGTGTGTG TATAGCAAAT CCTCTACAGG AACGAATTAT ATTAATGCGA
    AAGGAAGGTG AATCTGCAAA GAGTATTAAT GAGATGCTTC TGAGCAGGCT GTCCAGGTAC
    AGAGCCTCCC CATCAGCAAC ATTGGCTGCT CTCACTGGCT CTACCATCTC TAATACTCTA
    AAAGAGGACC AAGCAGCTGC TAACACAAGC TGCGGGCTGC CACTGAAAAT GTTAAGGAAG
    ACTCCGATAT ACACATGTGG AACATACCTG GTGATGTTGG TCCCACCCCC AGGAGGGTCA
    GGCAGTTCTG CAACACGCTC ACTCTTTGGA GGAACAAGTG CTTTGTCATC TTTAAAAATT
    CTTGCTTCAA GCTTGGTGTT CAATATTTCG GATGGGCAGT TCACAAGCCG AGCAGATCTC
    ATTGATGCTG CAGGAAGTTC ACTTGGACGT GGTGCTCTGG TACCAGGACT TGGTGCCTGT
    TATGATACTA TGAATAACAT GATATGGACG TGCTCTAATG ATTACATCGA TCAGTGGTGT
    AATCCTGGGA ACCAGGCATT CCACTGTGTC TGCCAGAGGC TGGGAGTGAG CCATGTCATT
    ACAGAGCCCA AAGGGGATGC AACAACCACA AATGAGGTTA TTAACCAGTT ACTGCACCAT
    GTTGGTGCCA TGTGTATCCA CCAACTCAAT CTGCTTGCAG CCAGCAATAA TCTTCCCATA
    ACAAACTTCT TGGGGAAGCA GCATCCAATC GAAGCACATC ATCTCAGCAG TATCTGTGAC
    ATTATGGAGA AAGCTATGGT GAACGGAGAT ACTTGTATAA TACGCTGCAT CCTTGTTGTC
    TTCCAGGTGG TTTTTAAATT TTTCTTCAGC CCACAAACTG AAAGAAACAG AGACATTGTG
    AGGAGGTCGG GCCTATTGCT TTGGCAGTTG TTGATGGCAC CAAGGGACCA GATTTGTGCG
    GAGATTCAGA AGGAGGTTTG CCTGGCTATA AGCTCTGGTT TAAATATCCT GTATCCTGGA
    GAAGCAGAAA TCAATAATTT ATTGAAATTG GTCTTAACTG AAGGAGAGAG AAATAGTGGT
    CTTTCTCAAC TTCGGGACGT CATCCTAACA AATCTAGCAG AACAGCTTCA GAACAACAGA
    TTTGGGAGTG AAGATGATGA TCATTATAGA CTAAATGATG AGCTGTTGCA CTACATTCTC
    AAGATTGTTG TCCGAGAATC TTGTGTCTTA ATCACCAAGT GCCAAACTGT ATCTAAAGAT
    GATTTTCAAA GGCTTCTTTC AACTGTGCCT GCTGCATCTT CATGCTTGCG ATATCTGATG
    GCCGTACAGA ACCACCTCCT CAGTAACACT ATTTTGATTA AACCTGATGA AAATGATGAC
    AGTGACAGCT CCTTACAGGG AGAGACATTG AAGGAGCTGA AGACCAGCGT TTTGTCTCTT
    GCTACACAAA TTCTGACAGG ATGTGATGAA GTCTTAGAAA TGCTGCAGCA AGTCACTACA
    GCACTAATAA ACAGTGATAT TGCTGACAGA GAACAAAGAT TAAAAGGCCT GGAACAAATT
    ACGAAAGCTA CTATGCTTGG TCATCTGCTT CCTGTCCTTC TGACATCCCT GATGCATCCA
    AATTTGCAAA CTCTAACCAT GGCTGATGCC TTGATGCCTC AGCTGGTGCA GCTGGTTCTC
    TATACAAGTC AGACTGCACT GCTGCTTAAA ACTCAGTCTC CGGTTTTCTC AGAACTGAAC
    AGCTCTCCTG GTGGTGTGTC GGAACACAAA GGGAAATTGT TACATGATGA GAGAATGCTG
    GAGGAGAAGG AAGAGCCAGG TTTTCTTACA GGCTTGAAGA TCCCTGCTCC TTGGGCTGCT
    GGGAAGACTG TAGAAACTGT TCATCCTGTC AGGGATAACT ATAAATTTAA GGAAACTGTA
    CACATACCAG GAGCCCGCTG TTTGTATCTT AGATTTGACA ACAGATGTTC TTCACAATAT
    GATTACGATA AGTTGGTGAT CTATGCTGGT CCTAACACAA ACAGTAGGAA GGTTGCTGAG
    TATGGAGGCA ATACACTGGG ATATGGCAGC CGTAGTGTCT TAGGAACTGG TTGGCCGAAA
    GACTTAGTAA AGGTGGAAGG AGATACAGTC ACCTTCTCCT TTGAAATGAG GAGTGGTCGT
    GAGCACAACA CTCCAGACAA AGCCATGTGG GGCTTTGCTT GCACTGTTCG AGCACAGGAG
    TCTTCAGAGG ATGTGTCAGG AGGCCTGCCA TTCTTAGTTG ACCTGGCACT TGGCCTTTCT
    GTGTTAGCTT GTTCTATGCT TCGGATTTTA TACAATGGAC CAGAAATTAC CAAAGATGAA
    GAAACCTGTC AAGAGCTTCT AAGATCTAAA CTATTACAAA GGTGCCAATG GCAGGTGGAA
    GCTAATGGTG TAATATCTCC AGCCCTTACA CCGAGTCCTT CTCCACTGCC TCTTACTATA
    GATGAGGACA GAGAATTTAC TTATCCTTCT GATGTGCTGG TGCCTCCCAT TGGACACTAT
    TTTGATCTGC CTAGGATTAG GCTGCCTCCA GGAATCATGA TAAAACTCAG GGAAATTTCT
    GGACGTGCTC GGCCTCAATT TAGACCTAGC ATAAAGGAAG TGATTCAGCC CGAGGTAATG
    GAGGAGATGG TAGTTTCATG TGTGATTAAA CATTTGAATT TGGTTGATGC ACTCCAGTCC
    CTGATAAACT TCCAGTATCA GGAAGAACAT GCTGAAGAAT ATGATTTACT TTGTAAAATT
    ATGGGAGAGA CTTTTAAGAA GCTAAATGCC ATGGAGAGAC AATTACAGAG TGTGGCTGAG
    CTGGAACAGA AATGGCAGAG TGAGGTTGAT GACGCCATGC ATGGGAAGCT GGAGAATAAT
    GTTCCCTTCT TCTATGATTA CCACTTCAAT GAGAGCAAGA TGAAAGAACT GGAACTTCTG
    TGTTCTATGA AGGAAGTTTC TTTTGATGGA AGTGATCTTG AGAATGTGGT CCTGGCATTA
    AGGGAGAAGT TCTTTCAAGA GGTGAATTCA CTGATACAGA AGACTTATCA TCCCCTAGCG
    AAAACAAAAG CATTAGTTAA AAGCTTGATG AACAGGGCAG AGCTGTTGCT GCATGTCACT
    ATTGCAGCGC AGTCTGGTAT CACAAGAAGT ATATCTGGAA CCCCTGCAGA GACTCCAGCC
    TGTAAGTCTG CATCTGAAAC AAAAGTGATA TCTCATGCAG TGCGCCAGCC CGTCTTCCTT
    CGCAGTATGT CAGCACCATC TGACTTAGAG ATGATTGGTA ACGAAGATAT TGAGTTTGCT
    CGATCAAGTC AAAGGCGACG CCATGTTACC AGCCACAGAA GTAGCTCTTT CACTCTTCTG
    CAATCACTGA CCATTGAGGA CAGCAGAGAT AAACCTACAT ACAGTGTCCT ACTGGGGCAG
    CTCTTTGCTT TCATTGGAAC TAATCCTGAC CAAGCGGTAT CCAGCAGCAG TTTTCTGCTC
    GCTGCTCAGA CAAGGTGGCG GCGTGGGAAC ACAAGGAAGC AAGCCTTAGT GCATATGAGA
    GAGCTGCTGA CTGCGGCTGT TCGGGTTGGT GGGGTGACAC ACCTCGTGGG CCCTGTGACC
    ATGGTACTTC AGGGAGGACC AAGAATTGAA GAGCTCACGT GTGGTGGGAT GGTGGAACAA
    GTCCAGGAAG CTTTTGGGGA GACTATGACA TCCGTGGTCT CGCTGTGTGC TCGCTACCCC
    ATTGCTTGTG CAAACAGCAT TGGCCTCTTA TGCACTATAC CTTACACAAG GAGTGAAGAG
    AAGTGTTTGG TACGTAGTGG CCTGGTACAG CTTATGGACA GGCTGTGCAG CTTAAGCAGC
    CAGGCAGAAT CCAGTTCAAA TGAAAAACAG ACCAAGAAGC AGAAGGTGGC CACCATGGCT
    TGGGCTGCCT TTCAGGTGCT GGCCAATCGC TGTGTTGAAT GGGAGAAAGA AGAAGGTGGT
    TCAACAGAAG CTGTTCACTC TGGTCTGGCT CGTCAGGTCT CCAGCCTTCT TACCAATCAC
    CTTGCACGAG CTACAGAGTG TTGTGGTAAT CAAGCTGCAG GAAATGATGC ACTTCAAGAC
    GTCCTCAGTC TTCTTAATGA CCTGTCGAGG AGTCACATAG GTAAAGCAAT CCTGAGCCAG
    CCAGCCTGTG TGTCCAAACT TCTGTCACTT CTCTTGGATC AACGTCCCTC TCCAAAGTTG
    GTTCTTATAA TCCTTCAGTT GTGTCGTGCT GCTCTACCTC TCATGAGTGT TGAAGACTGT
    GGAAATGTAG AACTGCCTCC CTGGAGTTAC TCTGTGCCCT CCCTGAACAG TGAACAAGAT
    GATCCCAGCG ACCCAGCCTC CAAGATTGCA TCTCTGCTCT TAGCAAAGCT GGCAGATTAT
    GTAGTACCAG GATGCCAGAC AGTTCTTTCT CCAACTGCTT CTGAACCAGA TACTGCTTTA
    GCAAAAAGCA GCCCTAAGAA TTCTATAAAA GGCGATAAAG ACCCTGGAGA AGAAAGTGAG
    GCAGTGGATG GCAAACTTTC TATTTTCATC CATAAACGGG AAGACCAGTC ATCCCACGAA
    GTGCTTCAGC CATTGCTGAG TAGCTCCGAA GGTCGCCCAT TCCGACTAGG AACTGGAGCC
    AACATGGAGA AGGTGGTGAA AATGGATCGT GACATGACTA AAAGTGGCTG CTGTGAAGTT
    ATCACAGAAG AAGCTGCTGC AGCTCTTCGC AAGGCAACTA AGTGGGCTCA GTCTGGTCTG
    ATAGTTAGTG TTGGGCCACC CATAGAAACT GTCAATCCAG AAGCAACCAG TGGGATGTCC
    TCTGGGGACA AAAAGAAGAC TGCACAGACA TCTATTTGCA GAGAGAGAAA TTCTGAGCTT
    GCCAGGACTG ATCCTGTAAG ACCATTCATC AGTGGTCATG TTGCAAATAG CATGGCAGCA
    GAAGTGATCG CTTTGCTACA TAGCTTGTTG ATGGCACCAG AATCAAATGC GGCTCAAATA
    TGGACAACAA CTGCTGAAAA AGTTCTGTCT CGGGCTCTGA TGTACATTCC ACAACTCGGG
    AAATACGCAG AGAGTATTTT GGAGAATGGA AGCAGCAGTG GAAGGAAGCT TGCTAAACTT
    CAAAGAATTG CTCGACAGGC AGTAGCTGCT CTTTGTGCTC TAGGTGGCTT TAAGGAGACA
    ATTAAAATCG GTTCTGAAGT TCAGGTTTTA GGTAAAGGAA TAGCAGGAAG CATTGGAGTT
    GTGGCATCTA TTAATGAACA AGAAGGTATA GCAACGGTCA AATTTCCGCC TACCACTATA
    GACAGTAGAA AGACCTCACA AGCATCAGAC ACTTTAACTA TTCCGTTATC TAGGCTCTGT
    GTTCCAAGAT CTGAGGCATT GCCTCTTCAT AAACTGTCCA TTACTGAGAA GGTAGTACAG
    GCAGTCCAGT CCATGTTGCT TCCTCAGGAG GGAAGTCTAT CTATTCACAC CTCACTTCCT
    GCAACAGGAG ATGGCTCAAC TCCAGTAATG GCAGTGGTCC GGCTCCTTGC TGAAATAAGA
    ACCAGAGCAT GCCTGGTGAT GGCTCAGCTG TTAGAAGACA GTTCATTCTG TGAAGAATTC
    ATTCAACAGT GCCCAGCTGC TGTAGAGGTT CTTAATTTGG TAGCCCAGGA GTGCAGCCCT
    GGAGAGAGGC TCACTGTAGT AGAAATGCAG TGTGAAAGGT TAAGGATGTT GTATCGTGAC
    TGTGCTCGAC CCCCACCTCC TCCACTGCAG GCAGACAGAA GGCAGCCCAA AGAAATCACT
    TGGAGCCCAT CAAGAGTGTT TCCCCCAGTA CGAGCATGCA TGTTTTCATC ACATCTTGCT
    GCTGTGACCT TCCTGGCTGA TCCAAGTGCA GGTGGGGGAC TTCCTCGGGG AACGTTCATT
    TATGCCACTT CTCCAGTTCC CGTGCAGGCA CCATCATTCT ACTGGGAAAT TGAAATAGTT
    TCCTATGGAG ATACAGATGA TGACACTGGT CCAATAGTTT CATTTGGATT CGCTACAGAG
    GCAGAAAAAC GGGATGGGGC TTGGACAAAC CCAGTGGGCA CCTGTCTTTT TCATAACAAT
    GGGCGAGCAG TGCATTACAA TGGCTCCAGC TTGTTACAGT GGAAGAGTGT TCGATTGGAT
    GTGACTCTTT CTCCTGGTGA TGTGGCTGGA ATTGGATGGG AAAGAGCAGA AGGAACTCCA
    CCCCCACCAG GGCAGCCTGC TAAAGGCAGA GTTTATTTTA CTTATTGCGG GCAGCGACTT
    GGGCCATACC TAGAGGATGT CTCTGGTGGA ATGTGGCCAA TTGTTCACAT ACAGAAAAAG
    AACACAAAAG TCCGTGCTAA TTTTGGATCT CGCCAGTTTG CCTATGCCGA AGGCCAAGCT
    CACAGGAATG CTGCTGATCT GTGTATTGAC CTAGCTGAAG AGATAAGTGC CAACTTTGAG
    GCATTGCCAT TTGCTATGGC TTCTGATAGC GATAACGACG CTGGCACCAG CATAGCTTCT
    GATCCTGGGA CTCACGGACC TCCCTGTAGA ATTGCTGCTG TTGCTACGGC TCAACAACAA
    TATGACAGTG ACACATCTTG TCACTATAAA ATGGAACTAA GCTATGAGAA CTTCATCACA
    TCAGGGCCTG ATCCTCATCC CCCTCCTATT GCAGATGATG AAAGTGATGA TGACGACGAT
    GATGATATTC CCAGGGAGGA CCACTATGCC CTGCTGGTTA AAGCCTGGGA AACTAAAGTT
    TTCCCTACAA TTAGAAGAAG ATTCCGTAAT GAGGCTGAGA GGAAATCTGG GCTGGATCAA
    ATTAAAGGAG CCTTACAGTT AGGAATGGTT GATATAGCAC GACAAACTGT AGAATTCTTG
    TATGAGGAAA ACGGTGGCAT CCCGAGAGAC CTCTACCTTC CTACCATTGA GGATATCAAA
    GATGAAGCAA ACAAGTTTAC AATCGATAAA GTACGAAAAG GTCTCACTGT TGTAACTCGC
    TCTCCTGACA GTAACAACGT TACCAGCAGT GCTGTTGGAA CAGCTTTACC CAAGTTTGCT
    ATTCGGGGAA TGCTGAAGAC ATTTGGTCTT CATGGTGTTG TCCTGGATGT TGATTCAGTG
    AATGAATTGG TACAAGTAGA GACCTATCTC AGAAGTGAGG GAGTTCTGGT AAGATATTGG
    TATCCTATTG ACATGTTAGA AAGGCCACCT GCTGGTTATA GGAGGACTGC AACAAATGGA
    TTGGTTACCC TGGACAATAC CAATATCCAA ATCCACAGAG AGCTTTTGAG AAGTGAAGCT
    GCACTGGCCA AGCTGTACTG CCGCATGGCT CTCCTCAATA TCTTTGCCCC CAAATCTCCA
    CATATGTTCA CCCGTCTGTT TCATATCCCC GCTATCCGTG ATATCACTCT GGAGCACCTG
    CAGTTGCTAT CCAACCAACT TCTCGCACCA CCTCTTCCTG ATGGAACAAT CAGTTCAAGT
    TCCATTCTTT TGGCTCAGTC ACTGCAACAC TGCATCCATT CCCAGAACTG TGCTGCCACA
    GACCTCTTCT ACCAGGGCAA TTCACAAGCC ATCAGAGAGT GGCTTACTGT TGCCATCACT
    CGGACACTGC ATCAAGGAGA GGAGAGCCTG TTAGACCTCA CTAAGCAGAT CTGCTCTTTT
    TTGCAGACTG CACCAGAACA GTTTCCTGCT GAAGAATTCC CAATTTCAGA ATCCAAAGTC
    AACATGGATG TTAATTTTCC TGGGGCTGCG TTTGTAATTG TGTCCTGTAA AGAAAGCCAG
    TCTGGATTCC GCAAAGATTC TTCCCTGTAT AAAGCTCCTT GGGCTCGGGT GCTTGTGTAC
    GGGCTGGGGC ACAAAGTTAA GAGGAATGGC CAGTTAAACC TGATTGAGGC AGTGTGTTAC
    CCTCGAGACG CCTCTCCGAC CAACACAGGC CTAACACCTC CCCCAACAAC CAATCAGTAC
    CCTTCAGTGA TCACTCCTAC AGACAAAGTG CACATCAAAC TAGGAGTGTC TCCTCCTCCT
    GGAGCTGTGC TGGTCTTGCA TTCTCTTCCC CTTGAGTTCC CCCTGGCAAT GGCATTTACA
    GAACAGCTGT TAACTTGGAA ACTGGAAGAT GTGGATGGGA AATCAGAAGA TGAACTGGAT
    ACTATTCCTC CTTCAGTTCT TTTGCAAGTA GTGGAATTTT TAGGGAACTT TCTGTGGACA
    ACTGACATGG CAGCATCTGT AAAGGAATTG ATATTTCATC TCTTGGCTGA ATTGCTTCGC
    AAAATTCACA ACCTGGAGCA GAAGAAAAGT CCTGCAGGAT TATCATCTTC TATTGCCCTT
    CAGCTGAATC CCTGTCTAGC TATGCTTATG GCCCTGCAGT CAGAACTCCA TAAGCTGTAC
    GATGAGGAAA CACAAAACTG GGTGTCTGGA AATGCATGCG GTGGTTCTGG TGTTGGAGCT
    GCTGACCAAG GAAGATTTTC AACATACTTC CATGCTCTGA TGGAAGTTTG CCTTGCTGTA
    GCAGAAGTAA CTCTACCTAT CAATATGAGC GTAACAGCTA ATGTGATATC TTCAACGAGT
    GCACCAAATC TTAGTGACTC TTCCTCCTCA TCTTCATCTT CTCCAGGACA GACACCGCAG
    AGTCCAAGCT TATTGTCAAA GAGGAAAAAA GTCAAAATGA AGAGAGAAAA GACATCGTCT
    TCAGGCAAAC GATCTTCATC CAGAGCAGCT GAAACGGATG CTGCAATCCT CAGTATAGGA
    GGAAGCAAGC CTGAGGATAT GCTATGGTTT CATAGAGCAC TGACTTTGCT TATCATTCTC
    AGACACCTAA CGAAAAAGGA TCCGCAAGGG TTGGGTGTGA CAAACGATGC TGTAGCAGAT
    GCATGTCAGG CACTGGTAGG ACCCACAGCT CATAGCCGTT TGCTTGTCAT TTCTGGAATA
    CCAACTCACT TGGAGGAGAG TACTGTCAGA AATGCAATCA GGAAGGCGTG TAATGCGCAT
    GGTGGAGTCT TCAAGGATGA AATCTACATC CCTCTGCAGG AAGAGGATCC CAAAAAAATC
    AAGGACAAAG CAGAAGGAGG AGAATGCAGA ACTGAGCTGG AAAAGCCAAC TGTTTCAAGT
    AGTGCGGACA GTCTGGATAT CAGCAGCTCT AGCAGTGTCA CACCTGCTAT GAGCGTTAGT
    GCGTCAGCAT CAACGAGCCA GGCTTCCCTG TGCAGCTCAC AGGGTATCTC CCGGACTGTT
    AGTGACATCT CTGTTGATCA GTACCAGGCT GGGCTGGAAC TGGCCATCCC ACCAGGACTG
    CTGGAGCCAC ACGTTTCCAG CCAGGAGAGC TTGGATCTGT CGCACTGCAG TACTGGGAGC
    CTTGGCAGCC TTGGCAGCCT CGGGGAGCCG CTTGACAATG TAGAGACAGC ATCAGTGTCT
    GATGTCGGGT CGATGTACAC GGTCACATCT CTGGACAACC AGCAACTTCT GTCACGGCCT
    ATCAAAGGTT TTGCTGTGGT GGAGATACGA TCAAGAGCTA AAATAGAGAA AATCCGAGCC
    AGCCTATTTA ACAGTAGTGA TCTCATTGGT TTGTCAAGTC TGGATGGTGA AGACGAACTG
    ATGGAAATGT CAACTGAAGA GATTTTAACT GTTTCTACTG TAAATCAGAG TTTATTTGAC
    ACACAAGGGA GCCCGGCGCT AGAAGACTAC TTCAATGATA AGTCAATAAA AGGTGAGAAA
    TTGGTTCCTG GTGCTCGGGA GGTCCTAACA GAAATATTTA AAAGCTGTGT CCATTCTGAG
    CAAATGCTGA GCCTGACTCC AACAAAACCA ATCAAGGTTG CTGATATCTA TCTTAGTAAA
    GAGCAGATAA ATTCTCAGAC TCCTGGAAAC CTTCTTCATG TCTTCTTCAC AAATGTCCGT
    CCTCCAAAGA AGGTGCTGGA GGACCAGCTT ACTCAGATTC TAAGGAAATA CGGTGTGCCC
    AAGCCGAAAT TTGACAAGAG CAAATACAAC AAGGCAGGCA AAGAACAGCA TCCGGTGAAG
    GTTGTGAGCA CCAAGCGCCC AGTTACAAAA CCACCCACAA AAGAAAAGTC TGTACTCAAC
    AGCGTCAGCC GAACTGCTTT GAGTGAGAAG AAACCTACTG TAAAGCCAAA GTCTCCTGAG
    AAGAGCAAAC CTGAAGAGAA GGACCCTGAA AAGTCTCCCA CAAAGAAACA AGAAGTTCCT
    GAGGAGAAGT ACCTGACACT GGATGGATTT CACAGATTTG TCATTGACCG ATCTAAGCAG
    GATATCCGTA GTGTGTGGAG AGCTATTCTG TCCTGTGGAT ATGATCTGCA TTTTGAAAGA
    TGTGCCTGCA TTGATGCAAG ACATGCACAG AAAGCATCTC GGAAGTGGAC TCTGGAAATG
    GACGTGGCTC TCGTTCAGTA CATTAACAGG CTTTGTCGTC ACCTCGCAAT TACACCAGCA
    CGACTCCATC CACATGAAGT ATATTTGGAT CCAGCTGATG CAGCAGATCC TAGAATCTCC
    TGTCTGTTGA ATGTTCCCGT TGAAAGTCTC CGCCTGCGGT TTGCACTGCT TCAGTCTCTC
    AATACGACGT TAGAGACGTT TTTCTTACCG CTGGTAGAGC TTCGGCAGAC TGAGATGTAC
    AGTAACAGCA TTGCTGCGCT GCTGCAGGAG GCCAAAGGTT TAATCTTCTA TGACACAAAA
    ATAACCGTAA TGAACAGAGT GCTGAATGCC ACTGTCCAAA GAACTGCTGA TCACGCAGCC
    CCAGAAATCA CCTTGGATCC CTTGGAAATA GTTGGAGGGG AGATCCGTTC TTCAGAAAAT
    TCCTACTTCT GCCAGGCAGC AAGGCAGCTG GCCTGTGTGC CATCCTCACA GCTCTGCGTC
    AAACTCGCCA GTGGTGGGGA CCCTACGTAC GCGTTCAACA TCCGGTTCAC AGGGGAGGAG
    GTCCATGGAA CAAGTGGATC TTTCCGTCAC TTCCTCTGGC AAGTTTGTAA GGAGCTGCAG
    AGCTCCTCGC TCTCTCTTCT CCTGTTGTGT CCAAGCTCTG CGGTCAACAA GAACAAGGGG
    AAGTACATTT TGACACCTAG CCCCATAACC TATGCAGAGG AGCAGCTGTT CCATTTCTTT
    GGGCAGCTTC TGGGCATTGC AATTCGAGCA GATGTTCCCC TGCCACTCGA CCTCCTGCCC
    TCATTCTGGA AGACTCTGGT AGGAGAGCCG CTGGATCCTG ATGTTGATCT CCAGGAAGCT
    GATATCCTCA CCTACAACTA CATTAAAAAG TTTGAAAATA TAAATGATGA AACTGAACTG
    GAAGCTCTGT GTGCAGAAAT TGCTTCTCAG CATTTAGCAA CAGAGAGCCC TGACTGTCCT
    AATAAGCCAT GCTGCAAATT CACCTACCTG ACCATGACAG GGGAAGAGGT GGAACTCTGT
    CCCAGGGGCA GGCACGTCCC AGTGGGATGG GAGAACAAGG ACGTGTATGC AGCTGCAATC
    AGGAGCTTGA GAATGCGTGA GCTGCAGACT CCAGAGTGCA TGACTGCAGT GCGTGCTGGG
    CTTGGGTCCA TCATTCCCCT GCAGCTTCTG ACCACGCTGA CCCCTCTTGA GATGGAGCTG
    AGGACATGTG GGCTTCCATA CATCAACCTG GAGTTTCTCA AGGCGCACAC GATGTACCAG
    GTGGGACTGA TGGAAACTGA TCAGCACATT GAGTTTTTTT GGAGCGCGCT GGAGATGTTC
    ACTCAGGAGG AGCTCTGCAA GTTCATAAAG TTTGCCTGTA ACCAGGAGCG GATCCCCTTC
    ACGTGTCCTT GCAAGGACGG CGGTCCCGAT ACCGCCCACG TCCCGCCTTA CCCCATGAAA
    ATAGCCCCCC CTGATGGAGC TGCAGGTTCT CCGGACTCTC GGTACATCCG TGTGGAAACG
    TGCATGTTCA TGATCAAACT TCCTCAGTAC TCATCACTGG ACATCATGCT GGAGAAGCTC
    CGATACGCCA TCCACTACCG GGAGGATCCT CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131029.1
    CDS418..13239
    Translation

    Target ORF information:

    RefSeq Version XM_015275543.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275543.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    ATGGGGCTTC TGCTGGTTTT TCCCCTCATT CAGTCTCAAA GTAGAACAGA CCCTTCTCTC 
    TGTAACATAA CCGCTGAGGT GCTATTGAAT TGCCTTCGAG ACTGCCAGCC TTTGAGCTTG
    ACTAAGGAGC CAGCTGACTG TCTCAATGGG ATAGAGTCTT TGCTGTGCTC GTGGTTGGAA
    GAAACTTCTG CTTCAGGTCA TCAGATTCCA TATAAGCAAA AGGAAAATGC TGCTGCTGCC
    CTAGTTGCCT TGGCTTGTGC AAGAGGGTCG CTGAAAACCT TTGTTCATAC AGTGCATCTA
    TTACAGAAAC AGACAGATCT GGGAACCTTG CCTGTGGCTG ATGTGTTATA CAGGTTGTTG
    CTTTTGGAGG GAGGTCCTGG CTCACCTTCT TGTTTGCTTG GAGGAAAGCA TATAGTGTCC
    TGGGGCTTTG AAGACATGTT ACCTGCACCT GATGCCAACA GTGGCTCCAG TGGTGAAAAT
    AAAGATGCAG ACTTAGGACG TTGTCTTGCA GCAGATGGCC TCTACCTTTA TACAACAAAT
    TCAGTGGGAA GAGGAATAAG CAAATTAGGA TCTGGATTAC ATGGTACCTT GCGAGGTTTT
    GTCTACTGCC GAAATGAGGA GCTGGAGGCT GGGTGGGTTG CTTTTGGCAA CAGCAAACTT
    CTTCATCGGC CTGTCTCTTT TGATAATAAG CCTCACTCCC TTTTCCAGGT TGTGGATCAG
    CATACCCTTC AGGTGTGTCA AATAATCCCA ATGCCAGTAA ATCACTTCCC AGTTGGCAGT
    ACTATGACCA CCGTGCATCT TTCTTCAGAT GGTACATATT TCTACTGGAT TTGGTCTCCT
    GCAAGCCTCA ATGAGAAAAC ACCAAAAGGC CACTCTGTCT TTATGGATGT TTTTGAACTT
    ATAGTAGAAA ATGGTGTGTG TATAGCAAAT CCTCTACAGG AACGAATTAT ATTAATGCGA
    AAGGAAGGTG AATCTGCAAA GAGTATTAAT GAGATGCTTC TGAGCAGGCT GTCCAGGTAC
    AGAGCCTCCC CATCAGCAAC ATTGGCTGCT CTCACTGGCT CTACCATCTC TAATACTCTA
    AAAGAGGACC AAGCAGCTGC TAACACAAGC TGCGGGCTGC CACTGAAAAT GTTAAGGAAG
    ACTCCGATAT ACACATGTGG AACATACCTG GTGATGTTGG TCCCACCCCC AGGAGGGTCA
    GGCAGTTCTG CAACACGCTC ACTCTTTGGA GGAACAAGTG CTTTGTCATC TTTAAAAATT
    CTTGCTTCAA GCTTGGTGTT CAATATTTCG GATGGGCAGT TCACAAGCCG AGCAGATCTC
    ATTGATGCTG CAGGAAGTTC ACTTGGACGT GGTGCTCTGG TACCAGGACT TGGTGCCTGT
    TATGATACTA TGAATAACAT GATATGGACG TGCTCTAATG ATTACATCGA TCAGTGGTGT
    AATCCTGGGA ACCAGGCATT CCACTGTGTC TGCCAGAGGC TGGGAGTGAG CCATGTCATT
    ACAGAGCCCA AAGGGGATGC AACAACCACA AATGAGGTTA TTAACCAGTT ACTGCACCAT
    GTTGGTGCCA TGTGTATCCA CCAACTCAAT CTGCTTGCAG CCAGCAATAA TCTTCCCATA
    ACAAACTTCT TGGGGAAGCA GCATCCAATC GAAGCACATC ATCTCAGCAG TATCTGTGAC
    ATTATGGAGA AAGCTATGGT GAACGGAGAT ACTTGTATAA TACGCTGCAT CCTTGTTGTC
    TTCCAGGTGG TTTTTAAATT TTTCTTCAGC CCACAAACTG AAAGAAACAG AGACATTGTG
    AGGAGGTCGG GCCTATTGCT TTGGCAGTTG TTGATGGCAC CAAGGGACCA GATTTGTGCG
    GAGATTCAGA AGGAGGTTTG CCTGGCTATA AGCTCTGGTT TAAATATCCT GTATCCTGGA
    GAAGCAGAAA TCAATAATTT ATTGAAATTG GTCTTAACTG AAGGAGAGAG AAATAGTGGT
    CTTTCTCAAC TTCGGGACGT CATCCTAACA AATCTAGCAG AACAGCTTCA GAACAACAGA
    TTTGGGAGTG AAGATGATGA TCATTATAGA CTAAATGATG AGCTGTTGCA CTACATTCTC
    AAGATTGTTG TCCGAGAATC TTGTGTCTTA ATCACCAAGT GCCAAACTGT ATCTAAAGAT
    GATTTTCAAA GGCTTCTTTC AACTGTGCCT GCTGCATCTT CATGCTTGCG ATATCTGATG
    GCCGTACAGA ACCACCTCCT CAGTAACACT ATTTTGATTA AACCTGATGA AAATGATGAC
    AGTGACAGCT CCTTACAGGG AGAGACATTG AAGGAGCTGA AGACCAGCGT TTTGTCTCTT
    GCTACACAAA TTCTGACAGG ATGTGATGAA GTCTTAGAAA TGCTGCAGCA AGTCACTACA
    GCACTAATAA ACAGTGATAT TGCTGACAGA GAACAAAGAT TAAAAGGCCT GGAACAAATT
    ACGAAAGCTA CTATGCTTGG TCATCTGCTT CCTGTCCTTC TGACATCCCT GATGCATCCA
    AATTTGCAAA CTCTAACCAT GGCTGATGCC TTGATGCCTC AGCTGGTGCA GCTGGTTCTC
    TATACAAGTC AGACTGCACT GCTGCTTAAA ACTCAGTCTC CGGTTTTCTC AGAACTGAAC
    AGCTCTCCTG GTGGTGTGTC GGAACACAAA GGGAAATTGT TACATGATGA GAGAATGCTG
    GAGGAGAAGG AAGAGCCAGG TTTTCTTACA GGCTTGAAGA TCCCTGCTCC TTGGGCTGCT
    GGGAAGACTG TAGAAACTGT TCATCCTGTC AGGGATAACT ATAAATTTAA GGAAACTGTA
    CACATACCAG GAGCCCGCTG TTTGTATCTT AGATTTGACA ACAGATGTTC TTCACAATAT
    GATTACGATA AGTTGGTGAT CTATGCTGGT CCTAACACAA ACAGTAGGAA GGTTGCTGAG
    TATGGAGGCA ATACACTGGG ATATGGCAGC CGTAGTGTCT TAGGAACTGG TTGGCCGAAA
    GACTTAGTAA AGGTGGAAGG AGATACAGTC ACCTTCTCCT TTGAAATGAG GAGTGGTCGT
    GAGCACAACA CTCCAGACAA AGCCATGTGG GGCTTTGCTT GCACTGTTCG AGCACAGGAG
    TCTTCAGAGG ATGTGTCAGG AGGCCTGCCA TTCTTAGTTG ACCTGGCACT TGGCCTTTCT
    GTGTTAGCTT GTTCTATGCT TCGGATTTTA TACAATGGAC CAGAAATTAC CAAAGATGAA
    GAAACCTGTC AAGAGCTTCT AAGATCTAAA CTATTACAAA GGTGCCAATG GCAGGTGGAA
    GCTAATGGTG TAATATCTCC AGCCCTTACA CCGAGTCCTT CTCCACTGCC TCTTACTATA
    GATGAGGACA GAGAATTTAC TTATCCTTCT GATGTGCTGG TGCCTCCCAT TGGACACTAT
    TTTGATCTGC CTAGGATTAG GCTGCCTCCA GGAATCATGA TAAAACTCAG GGAAATTTCT
    GGACGTGCTC GGCCTCAATT TAGACCTAGC ATAAAGGAAG TGATTCAGCC CGAGGTAATG
    GAGGAGATGG TAGTTTCATG TGTGATTAAA CATTTGAATT TGGTTGATGC ACTCCAGTCC
    CTGATAAACT TCCAGTATCA GGAAGAACAT GCTGAAGAAT ATGATTTACT TTGTAAAATT
    ATGGGAGAGA CTTTTAAGAA GCTAAATGCC ATGGAGAGAC AATTACAGAG TGTGGCTGAG
    CTGGAACAGA AATGGCAGAG TGAGGTTGAT GACGCCATGC ATGGGAAGCT GGAGAATAAT
    GTTCCCTTCT TCTATGATTA CCACTTCAAT GAGAGCAAGA TGAAAGAACT GGAACTTCTG
    TGTTCTATGA AGGAAGTTTC TTTTGATGGA AGTGATCTTG AGAATGTGGT CCTGGCATTA
    AGGGAGAAGT TCTTTCAAGA GGTGAATTCA CTGATACAGA AGACTTATCA TCCCCTAGCG
    AAAACAAAAG CATTAGTTAA AAGCTTGATG AACAGGGCAG AGCTGTTGCT GCATGTCACT
    ATTGCAGCGC AGTCTGGTAT CACAAGAAGT ATATCTGGAA CCCCTGCAGA GACTCCAGCC
    TGTAAGTCTG CATCTGAAAC AAAAGTGATA TCTCATGCAG TGCGCCAGCC CGTCTTCCTT
    CGCAGTATGT CAGCACCATC TGACTTAGAG ATGATTGGTA ACGAAGATAT TGAGTTTGCT
    CGATCAAGTC AAAGGCGACG CCATGTTACC AGCCACAGAA GTAGCTCTTT CACTCTTCTG
    CAATCACTGA CCATTGAGGA CAGCAGAGAT AAACCTACAT ACAGTGTCCT ACTGGGGCAG
    CTCTTTGCTT TCATTGGAAC TAATCCTGAC CAAGCGGTAT CCAGCAGCAG TTTTCTGCTC
    GCTGCTCAGA CAAGGTGGCG GCGTGGGAAC ACAAGGAAGC AAGCCTTAGT GCATATGAGA
    GAGCTGCTGA CTGCGGCTGT TCGGGTTGGT GGGGTGACAC ACCTCGTGGG CCCTGTGACC
    ATGGTACTTC AGGGAGGACC AAGAATTGAA GAGCTCACGT GTGGTGGGAT GGTGGAACAA
    GTCCAGGAAG CTTTTGGGGA GACTATGACA TCCGTGGTCT CGCTGTGTGC TCGCTACCCC
    ATTGCTTGTG CAAACAGCAT TGGCCTCTTA TGCACTATAC CTTACACAAG GAGTGAAGAG
    AAGTGTTTGG TACGTAGTGG CCTGGTACAG CTTATGGACA GGCTGTGCAG CTTAAGCAGC
    CAGGCAGAAT CCAGTTCAAA TGAAAAACAG ACCAAGAAGC AGAAGGTGGC CACCATGGCT
    TGGGCTGCCT TTCAGGTGCT GGCCAATCGC TGTGTTGAAT GGGAGAAAGA AGAAGGTGGT
    TCAACAGAAG CTGTTCACTC TGGTCTGGCT CGTCAGGTCT CCAGCCTTCT TACCAATCAC
    CTTGCACGAG CTACAGAGTG TTGTGGTAAT CAAGCTGCAG GAAATGATGC ACTTCAAGAC
    GTCCTCAGTC TTCTTAATGA CCTGTCGAGG AGTCACATAG GTAAAGCAAT CCTGAGCCAG
    CCAGCCTGTG TGTCCAAACT TCTGTCACTT CTCTTGGATC AACGTCCCTC TCCAAAGTTG
    GTTCTTATAA TCCTTCAGTT GTGTCGTGCT GCTCTACCTC TCATGAGTGT TGAAGACTGT
    GGAAATGTAG AACTGCCTCC CTGGAGTTAC TCTGTGCCCT CCCTGAACAG TGAACAAGAT
    GATCCCAGCG ACCCAGCCTC CAAGATTGCA TCTCTGCTCT TAGCAAAGCT GGCAGATTAT
    GTAGTACCAG GATGCCAGAC AGTTCTTTCT CCAACTGCTT CTGAACCAGA TACTGCTTTA
    GCAAAAAGCA GCCCTAAGAA TTCTATAAAA GGCGATAAAG ACCCTGGAGA AGAAAGTGAG
    GCAGTGGATG GCAAACTTTC TATTTTCATC CATAAACGGG AAGACCAGTC ATCCCACGAA
    GTGCTTCAGC CATTGCTGAG TAGCTCCGAA GGTCGCCCAT TCCGACTAGG AACTGGAGCC
    AACATGGAGA AGGTGGTGAA AATGGATCGT GACATGACTA AAAGTGGCTG CTGTGAAGTT
    ATCACAGAAG AAGCTGCTGC AGCTCTTCGC AAGGCAACTA AGTGGGCTCA GTCTGGTCTG
    ATAGTTAGTG TTGGGCCACC CATAGAAACT GTCAATCCAG AAGCAACCAG TGGGATGTCC
    TCTGGGGACA AAAAGAAGAC TGCACAGACA TCTATTTGCA GAGAGAGAAA TTCTGAGCTT
    GCCAGGACTG ATCCTGTAAG ACCATTCATC AGTGGTCATG TTGCAAATAG CATGGCAGCA
    GAAGTGATCG CTTTGCTACA TAGCTTGTTG ATGGCACCAG AATCAAATGC GGCTCAAATA
    TGGACAACAA CTGCTGAAAA AGTTCTGTCT CGGGCTCTGA TGTACATTCC ACAACTCGGG
    AAATACGCAG AGAGTATTTT GGAGAATGGA AGCAGCAGTG GAAGGAAGCT TGCTAAACTT
    CAAAGAATTG CTCGACAGGC AGTAGCTGCT CTTTGTGCTC TAGGTGGCTT TAAGGAGACA
    ATTAAAATCG GTTCTGAAGT TCAGGTTTTA GGTAAAGGAA TAGCAGGAAG CATTGGAGTT
    GTGGCATCTA TTAATGAACA AGAAGGTATA GCAACGGTCA AATTTCCGCC TACCACTATA
    GACAGTAGAA AGACCTCACA AGCATCAGAC ACTTTAACTA TTCCGTTATC TAGGCTCTGT
    GTTCCAAGAT CTGAGGCATT GCCTCTTCAT AAACTGTCCA TTACTGAGAA GGTAGTACAG
    GCAGTCCAGT CCATGTTGCT TCCTCAGGAG GGAAGTCTAT CTATTCACAC CTCACTTCCT
    GCAACAGGAG ATGGCTCAAC TCCAGTAATG GCAGTGGTCC GGCTCCTTGC TGAAATAAGA
    ACCAGAGCAT GCCTGGTGAT GGCTCAGCTG TTAGAAGACA GTTCATTCTG TGAAGAATTC
    ATTCAACAGT GCCCAGCTGC TGTAGAGGTT CTTAATTTGG TAGCCCAGGA GTGCAGCCCT
    GGAGAGAGGC TCACTGTAGT AGAAATGCAG TGTGAAAGGT TAAGGATGTT GTATCGTGAC
    TGTGCTCGAC CCCCACCTCC TCCACTGCAG GCAGACAGAA GGCAGCCCAA AGAAATCACT
    TGGAGCCCAT CAAGAGTGTT TCCCCCAGTA CGAGCATGCA TGTTTTCATC ACATCTTGCT
    GCTGTGACCT TCCTGGCTGA TCCAAGTGCA GGTGGGGGAC TTCCTCGGGG AACGTTCATT
    TATGCCACTT CTCCAGTTCC CGTGCAGGCA CCATCATTCT ACTGGGAAAT TGAAATAGTT
    TCCTATGGAG ATACAGATGA TGACACTGGT CCAATAGTTT CATTTGGATT CGCTACAGAG
    GCAGAAAAAC GGGATGGGGC TTGGACAAAC CCAGTGGGCA CCTGTCTTTT TCATAACAAT
    GGGCGAGCAG TGCATTACAA TGGCTCCAGC TTGTTACAGT GGAAGAGTGT TCGATTGGAT
    GTGACTCTTT CTCCTGGTGA TGTGGCTGGA ATTGGATGGG AAAGAGCAGA AGGAACTCCA
    CCCCCACCAG GGCAGCCTGC TAAAGGCAGA GTTTATTTTA CTTATTGCGG GCAGCGACTT
    GGGCCATACC TAGAGGATGT CTCTGGTGGA ATGTGGCCAA TTGTTCACAT ACAGAAAAAG
    AACACAAAAG TCCGTGCTAA TTTTGGATCT CGCCAGTTTG CCTATGCCGA AGGCCAAGCT
    CACAGGAATG CTGCTGATCT GTGTATTGAC CTAGCTGAAG AGATAAGTGC CAACTTTGAG
    GCATTGCCAT TTGCTATGGC TTCTGATAGC GATAACGACG CTGGCACCAG CATAGCTTCT
    GATCCTGGGA CTCACGGACC TCCCTGTAGA ATTGCTGCTG TTGCTACGGC TCAACAACAA
    TATGACAGTG ACACATCTTG TCACTATAAA ATGGAACTAA GCTATGAGAA CTTCATCACA
    TCAGGGCCTG ATCCTCATCC CCCTCCTATT GCAGATGATG AAAGTGATGA TGACGACGAT
    GATGATATTC CCAGGGAGGA CCACTATGCC CTGCTGGTTA AAGCCTGGGA AACTAAAGTT
    TTCCCTACAA TTAGAAGAAG ATTCCGTAAT GAGGCTGAGA GGAAATCTGG GCTGGATCAA
    ATTAAAGGAG CCTTACAGTT AGGAATGGTT GATATAGCAC GACAAACTGT AGAATTCTTG
    TATGAGGAAA ACGGTGGCAT CCCGAGAGAC CTCTACCTTC CTACCATTGA GGATATCAAA
    GATGAAGCAA ACAAGTTTAC AATCGATAAA GTACGAAAAG GTCTCACTGT TGTAACTCGC
    TCTCCTGACA GTAACAACGT TACCAGCAGT GCTGTTGGAA CAGCTTTACC CAAGTTTGCT
    ATTCGGGGAA TGCTGAAGAC ATTTGGTCTT CATGGTGTTG TCCTGGATGT TGATTCAGTG
    AATGAATTGG TACAAGTAGA GACCTATCTC AGAAGTGAGG GAGTTCTGGT AAGATATTGG
    TATCCTATTG ACATGTTAGA AAGGCCACCT GCTGGTTATA GGAGGACTGC AACAAATGGA
    TTGGTTACCC TGGACAATAC CAATATCCAA ATCCACAGAG AGCTTTTGAG AAGTGAAGCT
    GCACTGGCCA AGCTGTACTG CCGCATGGCT CTCCTCAATA TCTTTGCCCC CAAATCTCCA
    CATATGTTCA CCCGTCTGTT TCATATCCCC GCTATCCGTG ATATCACTCT GGAGCACCTG
    CAGTTGCTAT CCAACCAACT TCTCGCACCA CCTCTTCCTG ATGGAACAAT CAGTTCAAGT
    TCCATTCTTT TGGCTCAGTC ACTGCAACAC TGCATCCATT CCCAGAACTG TGCTGCCACA
    GACCTCTTCT ACCAGGGCAA TTCACAAGCC ATCAGAGAGT GGCTTACTGT TGCCATCACT
    CGGACACTGC ATCAAGGAGA GGAGAGCCTG TTAGACCTCA CTAAGCAGAT CTGCTCTTTT
    TTGCAGACTG CACCAGAACA GTTTCCTGCT GAAGAATTCC CAATTTCAGA ATCCAAAGTC
    AACATGGATG TTAATTTTCC TGGGGCTGCG TTTGTAATTG TGTCCTGTAA AGAAAGCCAG
    TCTGGATTCC GCAAAGATTC TTCCCTGTAT AAAGCTCCTT GGGCTCGGGT GCTTGTGTAC
    GGGCTGGGGC ACAAAGTTAA GAGGAATGGC CAGTTAAACC TGATTGAGGC AGTGTGTTAC
    CCTCGAGACG CCTCTCCGAC CAACACAGGC CTAACACCTC CCCCAACAAC CAATCAGTAC
    CCTTCAGTGA TCACTCCTAC AGACAAAGTG CACATCAAAC TAGGAGTGTC TCCTCCTCCT
    GGAGCTGTGC TGGTCTTGCA TTCTCTTCCC CTTGAGTTCC CCCTGGCAAT GGCATTTACA
    GAACAGCTGT TAACTTGGAA ACTGGAAGAT GTGGATGGGA AATCAGAAGA TGAACTGGAT
    ACTATTCCTC CTTCAGTTCT TTTGCAAGTA GTGGAATTTT TAGGGAACTT TCTGTGGACA
    ACTGACATGG CAGCATCTGT AAAGGAATTG ATATTTCATC TCTTGGCTGA ATTGCTTCGC
    AAAATTCACA ACCTGGAGCA GAAGAAAAGT CCTGCAGGAT TATCATCTTC TATTGCCCTT
    CAGCTGAATC CCTGTCTAGC TATGCTTATG GCCCTGCAGT CAGAACTCCA TAAGCTGTAC
    GATGAGGAAA CACAAAACTG GGTGTCTGGA AATGCATGCG GTGGTTCTGG TGTTGGAGCT
    GCTGACCAAG GAAGATTTTC AACATACTTC CATGCTCTGA TGGAAGTTTG CCTTGCTGTA
    GCAGAAGTAA CTCTACCTAT CAATATGAGC GTAACAGCTA ATGTGATATC TTCAACGAGT
    GCACCAAATC TTAGTGACTC TTCCTCCTCA TCTTCATCTT CTCCAGGACA GACACCGCAG
    AGTCCAAGCT TATTGTCAAA GAGGAAAAAA GTCAAAATGA AGAGAGAAAA GACATCGTCT
    TCAGGCAAAC GATCTTCATC CAGAGCAGCT GAAACGGATG CTGCAATCCT CAGTATAGGA
    GGAAGCAAGC CTGAGGATAT GCTATGGTTT CATAGAGCAC TGACTTTGCT TATCATTCTC
    AGACACCTAA CGAAAAAGGA TCCGCAAGGG TTGGGTGTGA CAAACGATGC TGTAGCAGAT
    GCATGTCAGG CACTGGTAGG ACCCACAGCT CATAGCCGTT TGCTTGTCAT TTCTGGAATA
    CCAACTCACT TGGAGGAGAG TACTGTCAGA AATGCAATCA GGAAGGCGTG TAATGCGCAT
    GGTGGAGTCT TCAAGGATGA AATCTACATC CCTCTGCAGG AAGAGGATCC CAAAAAAATC
    AAGGACAAAG CAGAAGGAGG AGAATGCAGA ACTGAGCTGG AAAAGCCAAC TGTTTCAAGT
    AGTGCGGACA GTCTGGATAT CAGCAGCTCT AGCAGTGTCA CACCTGCTAT GAGCGTTAGT
    GCGTCAGCAT CAACGAGCCA GGCTTCCCTG TGCAGCTCAC AGGGTATCTC CCGGACTGTT
    AGTGACATCT CTGTTGATCA GTACCAGGCT GGGCTGGAAC TGGCCATCCC ACCAGGACTG
    CTGGAGCCAC ACGTTTCCAG CCAGGAGAGC TTGGATCTGT CGCACTGCAG TACTGGGAGC
    CTTGGCAGCC TTGGCAGCCT CGGGGAGCCG CTTGACAATG TAGAGACAGC ATCAGTGTCT
    GATGTCGGGT CGATGTACAC GGTCACATCT CTGGACAACC AGCAACTTCT GTCACGGCCT
    ATCAAAGGTT TTGCTGTGGT GGAGATACGA TCAAGAGCTA AAATAGAGAA AATCCGAGCC
    AGCCTATTTA ACAGTAGTGA TCTCATTGGT TTGTCAAGTC TGGATGGTGA AGACGAACTG
    ATGGAAATGT CAACTGAAGA GATTTTAACT GTTTCTACTG TAAATCAGAG TTTATTTGAC
    ACACAAGGGA GCCCGGCGCT AGAAGACTAC TTCAATGATA AGTCAATAAA AGGTGAGAAA
    TTGGTTCCTG GTGCTCGGGA GGTCCTAACA GAAATATTTA AAAGCTGTGT CCATTCTGAG
    CAAATGCTGA GCCTGACTCC AACAAAACCA ATCAAGGTTG CTGATATCTA TCTTAGTAAA
    GAGCAGATAA ATTCTCAGAC TCCTGGAAAC CTTCTTCATG TCTTCTTCAC AAATGTCCGT
    CCTCCAAAGA AGGTGCTGGA GGACCAGCTT ACTCAGATTC TAAGGAAATA CGGTGTGCCC
    AAGCCGAAAT TTGACAAGAG CAAATACAAC AAGGCAGGCA AAGAACAGCA TCCGGTGAAG
    GTTGTGAGCA CCAAGCGCCC AGTTACAAAA CCACCCACAA AAGAAAAGTC TGTACTCAAC
    AGCGTCAGCC GAACTGCTTT GAGTGAGAAG AAACCTACTG TAAAGCCAAA GTCTCCTGAG
    AAGAGCAAAC CTGAAGAGAA GGACCCTGAA AAGTCTCCCA CAAAGAAACA AGAAGTTCCT
    GAGGAGAAGT ACCTGACACT GGATGGATTT CACAGATTTG TCATTGACCG ATCTAAGCAG
    GATATCCGTA GTGTGTGGAG AGCTATTCTG TCCTGTGGAT ATGATCTGCA TTTTGAAAGA
    TGTGCCTGCA TTGATGCAAG ACATGCACAG AAAGCATCTC GGAAGTGGAC TCTGGAAATG
    GACGTGGCTC TCGTTCAGTA CATTAACAGG CTTTGTCGTC ACCTCGCAAT TACACCAGCA
    CGACTCCATC CACATGAAGT ATATTTGGAT CCAGCTGATG CAGCAGATCC TAGAATCTCC
    TGTCTGTTGA ATGTTCCCGT TGAAAGTCTC CGCCTGCGGT TTGCACTGCT TCAGTCTCTC
    AATACGACGT TAGAGACGTT TTTCTTACCG CTGGTAGAGC TTCGGCAGAC TGAGATGTAC
    AGTAACAGCA TTGCTGCGCT GCTGCAGGAG GCCAAAGGTT TAATCTTCTA TGACACAAAA
    ATAACCGTAA TGAACAGAGT GCTGAATGCC ACTGTCCAAA GAACTGCTGA TCACGCAGCC
    CCAGAAATCA CCTTGGATCC CTTGGAAATA GTTGGAGGGG AGATCCGTTC TTCAGAAAAT
    TCCTACTTCT GCCAGGCAGC AAGGCAGCTG GCCTGTGTGC CATCCTCACA GCTCTGCGTC
    AAACTCGCCA GTGGTGGGGA CCCTACGTAC GCGTTCAACA TCCGGTTCAC AGGGGAGGAG
    GTCCATGGAA CAAGTGGATC TTTCCGTCAC TTCCTCTGGC AAGTTTGTAA GGAGCTGCAG
    AGCTCCTCGC TCTCTCTTCT CCTGTTGTGT CCAAGCTCTG CGGTCAACAA GAACAAGGGG
    AAGTACATTT TGACACCTAG CCCCATAACC TATGCAGAGG AGCAGCTGTT CCATTTCTTT
    GGGCAGCTTC TGGGCATTGC AATTCGAGCA GATGTTCCCC TGCCACTCGA CCTCCTGCCC
    TCATTCTGGA AGACTCTGGT AGGAGAGCCG CTGGATCCTG ATGTTGATCT CCAGGAAGCT
    GATATCCTCA CCTACAACTA CATTAAAAAG TTTGAAAATA TAAATGATGA AACTGAACTG
    GAAGCTCTGT GTGCAGAAAT TGCTTCTCAG CATTTAGCAA CAGAGAGCCC TGACTGTCCT
    AATAAGCCAT GCTGCAAATT CACCTACCTG ACCATGACAG GGGAAGAGGT GGAACTCTGT
    CCCAGGGGCA GGCACGTCCC AGTGGGATGG GAGAACAAGG ACGTGTATGC AGCTGCAATC
    AGGAGCTTGA GAATGCGTGA GCTGCAGACT CCAGAGTGCA TGACTGCAGT GCGTGCTGGG
    CTTGGGTCCA TCATTCCCCT GCAGCTTCTG ACCACGCTGA CCCCTCTTGA GATGGAGCTG
    AGGACATGTG GGCTTCCATA CATCAACCTG GAGTTTCTCA AGGCGCACAC GATGTACCAG
    GTGGGACTGA TGGAAACTGA TCAGCACATT GAGTTTTTTT GGAGCGCGCT GGAGATGTTC
    ACTCAGGAGG AGCTCTGCAA GTTCATAAAG TTTGCCTGTA ACCAGGAGCG GATCCCCTTC
    ACGTGTCCTT GCAAGGACGG CGGTCCCGAT ACCGCCCACG TCCCGCCTTA CCCCATGAAA
    ATAGCCCCCC CTGATGGAGC TGCAGGTTCT CCGGACTCTC GGTACATCCG TGTGGAAACG
    TGCATGTTCA TGATCAAACT TCCTCAGTAC TCATCACTGG ACATCATGCT GGAGAAGCTC
    CGATACGCCA TCCACTACCG GGAGGATCCT CTCAGCGGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa42928
    Clone ID Related Accession (Same CDS sequence) XM_015275544.1
    Accession Version XM_015275544.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13245bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001471461.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
    2461
    2521
    2581
    2641
    2701
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    4981
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
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    6301
    6361
    6421
    6481
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    7141
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
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    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TCTAACAGTG TATTTTATGT TTTGAAGTTG
    GTGATCTATG CTGGTCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT AGTAAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGGAGTG GTCGTGAGCA CAACACTCCA
    GACAAAGCCA TGTGGGGCTT TGCTTGCACT GTTCGAGCAC AGGAGTCTTC AGAGGATGTG
    TCAGGAGGCC TGCCATTCTT AGTTGACCTG GCACTTGGCC TTTCTGTGTT AGCTTGTTCT
    ATGCTTCGGA TTTTATACAA TGGACCAGAA ATTACCAAAG ATGAAGAAAC CTGTCAAGAG
    CTTCTAAGAT CTAAACTATT ACAAAGGTGC CAATGGCAGG TGGAAGCTAA TGGTGTAATA
    TCTCCAGCCC TTACACCGAG TCCTTCTCCA CTGCCTCTTA CTATAGATGA GGACAGAGAA
    TTTACTTATC CTTCTGATGT GCTGGTGCCT CCCATTGGAC ACTATTTTGA TCTGCCTAGG
    ATTAGGCTGC CTCCAGGAAT CATGATAAAA CTCAGGGAAA TTTCTGGACG TGCTCGGCCT
    CAATTTAGAC CTAGCATAAA GGAAGTGATT CAGCCCGAGG TAATGGAGGA GATGGTAGTT
    TCATGTGTGA TTAAACATTT GAATTTGGTT GATGCACTCC AGTCCCTGAT AAACTTCCAG
    TATCAGGAAG AACATGCTGA AGAATATGAT TTACTTTGTA AAATTATGGG AGAGACTTTT
    AAGAAGCTAA ATGCCATGGA GAGACAATTA CAGAGTGTGG CTGAGCTGGA ACAGAAATGG
    CAGAGTGAGG TTGATGACGC CATGCATGGG AAGCTGGAGA ATAATGTTCC CTTCTTCTAT
    GATTACCACT TCAATGAGAG CAAGATGAAA GAACTGGAAC TTCTGTGTTC TATGAAGGAA
    GTTTCTTTTG ATGGAAGTGA TCTTGAGAAT GTGGTCCTGG CATTAAGGGA GAAGTTCTTT
    CAAGAGGTGA ATTCACTGAT ACAGAAGACT TATCATCCCC TAGCGAAAAC AAAAGCATTA
    GTTAAAAGCT TGATGAACAG GGCAGAGCTG TTGCTGCATG TCACTATTGC AGCGCAGTCT
    GGTATCACAA GAAGTATATC TGGAACCCCT GCAGAGACTC CAGTGCGCCA GCCCGTCTTC
    CTTCGCAGTA TGTCAGCACC ATCTGACTTA GAGATGATTG GTAACGAAGA TATTGAGTTT
    GCTCGATCAA GTCAAAGGCG ACGCCATGTT ACCAGCCACA GAAGTAGCTC TTTCACTCTT
    CTGCAATCAC TGACCATTGA GGACAGCAGA GATAAACCTA CATACAGTGT CCTACTGGGG
    CAGCTCTTTG CTTTCATTGG AACTAATCCT GACCAAGCGG TATCCAGCAG CAGTTTTCTG
    CTCGCTGCTC AGACAAGGTG GCGGCGTGGG AACACAAGGA AGCAAGCCTT AGTGCATATG
    AGAGAGCTGC TGACTGCGGC TGTTCGGGTT GGTGGGGTGA CACACCTCGT GGGCCCTGTG
    ACCATGGTAC TTCAGGGAGG ACCAAGAATT GAAGAGCTCA CGTGTGGTGG GATGGTGGAA
    CAAGTCCAGG AAGCTTTTGG GGAGACTATG ACATCCGTGG TCTCGCTGTG TGCTCGCTAC
    CCCATTGCTT GTGCAAACAG CATTGGCCTC TTATGCACTA TACCTTACAC AAGGAGTGAA
    GAGAAGTGTT TGGTACGTAG TGGCCTGGTA CAGCTTATGG ACAGGCTGTG CAGCTTAAGC
    AGCCAGGCAG AATCCAGTTC AAATGAAAAA CAGACCAAGA AGCAGAAGGT GGCCACCATG
    GCTTGGGCTG CCTTTCAGGT GCTGGCCAAT CGCTGTGTTG AATGGGAGAA AGAAGAAGGT
    GGTTCAACAG AAGCTGTTCA CTCTGGTCTG GCTCGTCAGG TCTCCAGCCT TCTTACCAAT
    CACCTTGCAC GAGCTACAGA GTGTTGTGGT AATCAAGCTG CAGGAAATGA TGCACTTCAA
    GACGTCCTCA GTCTTCTTAA TGACCTGTCG AGGAGTCACA TAGGTAAAGC AATCCTGAGC
    CAGCCAGCCT GTGTGTCCAA ACTTCTGTCA CTTCTCTTGG ATCAACGTCC CTCTCCAAAG
    TTGGTTCTTA TAATCCTTCA GTTGTGTCGT GCTGCTCTAC CTCTCATGAG TGTTGAAGAC
    TGTGGAAATG TAGAACTGCC TCCCTGGAGT TACTCTGTGC CCTCCCTGAA CAGTGAACAA
    GATGATCCCA GCGACCCAGC CTCCAAGATT GCATCTCTGC TCTTAGCAAA GCTGGCAGAT
    TATGTAGTAC CAGGATGCCA GACAGTTCTT TCTCCAACTG CTTCTGAACC AGATACTGCT
    TTAGCAAAAA GCAGCCCTAA GAATTCTATA AAAGGCGATA AAGACCCTGG AGAAGAAAGT
    GAGGCAGTGG ATGGCAAACT TTCTATTTTC ATCCATAAAC GGGAAGACCA GTCATCCCAC
    GAAGTGCTTC AGCCATTGCT GAGTAGCTCC GAAGGTCGCC CATTCCGACT AGGAACTGGA
    GCCAACATGG AGAAGGTGGT GAAAATGGAT CGTGACATGA CTAAAAGTGG CTGCTGTGAA
    GTTATCACAG AAGAAGCTGC TGCAGCTCTT CGCAAGGCAA CTAAGTGGGC TCAGTCTGGT
    CTGATAGTTA GTGTTGGGCC ACCCATAGAA ACTGTCAATC CAGAAGCAAC CAGTGGGATG
    TCCTCTGGGG ACAAAAAGAA GACTGCACAG ACATCTATTT GCAGAGAGAG AAATTCTGAG
    CTTGCCAGGA CTGATCCTGT AAGACCATTC ATCAGTGGTC ATGTTGCAAA TAGCATGGCA
    GCAGAAGTGA TCGCTTTGCT ACATAGCTTG TTGATGGCAC CAGAATCAAA TGCGGCTCAA
    ATATGGACAA CAACTGCTGA AAAAGTTCTG TCTCGGGCTC TGATGTACAT TCCACAACTC
    GGGAAATACG CAGAGAGTAT TTTGGAGAAT GGAAGCAGCA GTGGAAGGAA GCTTGCTAAA
    CTTCAAAGAA TTGCTCGACA GGCAGTAGCT GCTCTTTGTG CTCTAGGTGG CTTTAAGGAG
    ACAATTAAAA TCGGTTCTGA AGTTCAGGTT TTAGGTAAAG GAATAGCAGG AAGCATTGGA
    GTTGTGGCAT CTATTAATGA ACAAGAAGGT ATAGCAACGG TCAAATTTCC GCCTACCACT
    ATAGACAGTA GAAAGACCTC ACAAGCATCA GACACTTTAA CTATTCCGTT ATCTAGGCTC
    TGTGTTCCAA GATCTGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TATCTATTCA CACCTCACTT
    CCTGCAACAG GAGATGGCTC AACTCCAGTA ATGGCAGTGG TCCGGCTCCT TGCTGAAATA
    AGAACCAGAG CATGCCTGGT GATGGCTCAG CTGTTAGAAG ACAGTTCATT CTGTGAAGAA
    TTCATTCAAC AGTGCCCAGC TGCTGTAGAG GTTCTTAATT TGGTAGCCCA GGAGTGCAGC
    CCTGGAGAGA GGCTCACTGT AGTAGAAATG CAGTGTGAAA GGTTAAGGAT GTTGTATCGT
    GACTGTGCTC GACCCCCACC TCCTCCACTG CAGGCAGACA GAAGGCAGCC CAAAGAAATC
    ACTTGGAGCC CATCAAGAGT GTTTCCCCCA GTACGAGCAT GCATGTTTTC ATCACATCTT
    GCTGCTGTGA CCTTCCTGGC TGATCCAAGT GCAGGTGGGG GACTTCCTCG GGGAACGTTC
    ATTTATGCCA CTTCTCCAGT TCCCGTGCAG GCACCATCAT TCTACTGGGA AATTGAAATA
    GTTTCCTATG GAGATACAGA TGATGACACT GGTCCAATAG TTTCATTTGG ATTCGCTACA
    GAGGCAGAAA AACGGGATGG GGCTTGGACA AACCCAGTGG GCACCTGTCT TTTTCATAAC
    AATGGGCGAG CAGTGCATTA CAATGGCTCC AGCTTGTTAC AGTGGAAGAG TGTTCGATTG
    GATGTGACTC TTTCTCCTGG TGATGTGGCT GGAATTGGAT GGGAAAGAGC AGAAGGAACT
    CCACCCCCAC CAGGGCAGCC TGCTAAAGGC AGAGTTTATT TTACTTATTG CGGGCAGCGA
    CTTGGGCCAT ACCTAGAGGA TGTCTCTGGT GGAATGTGGC CAATTGTTCA CATACAGAAA
    AAGAACACAA AAGTCCGTGC TAATTTTGGA TCTCGCCAGT TTGCCTATGC CGAAGGCCAA
    GCTCACAGGA ATGCTGCTGA TCTGTGTATT GACCTAGCTG AAGAGATAAG TGCCAACTTT
    GAGGCATTGC CATTTGCTAT GGCTTCTGAT AGCGATAACG ACGCTGGCAC CAGCATAGCT
    TCTGATCCTG GGACTCACGG ACCTCCCTGT AGAATTGCTG CTGTTGCTAC GGCTCAACAA
    CAATATGACA GTGACACATC TTGTCACTAT AAAATGGAAC TAAGCTATGA GAACTTCATC
    ACATCAGGGC CTGATCCTCA TCCCCCTCCT ATTGCAGATG ATGAAAGTGA TGATGACGAC
    GATGATGATA TTCCCAGGGA GGACCACTAT GCCCTGCTGG TTAAAGCCTG GGAAACTAAA
    GTTTTCCCTA CAATTAGAAG AAGATTCCGT AATGAGGCTG AGAGGAAATC TGGGCTGGAT
    CAAATTAAAG GAGCCTTACA GTTAGGAATG GTTGATATAG CACGACAAAC TGTAGAATTC
    TTGTATGAGG AAAACGGTGG CATCCCGAGA GACCTCTACC TTCCTACCAT TGAGGATATC
    AAAGATGAAG CAAACAAGTT TACAATCGAT AAAGTACGAA AAGGTCTCAC TGTTGTAACT
    CGCTCTCCTG ACAGTAACAA CGTTACCAGC AGTGCTGTTG GAACAGCTTT ACCCAAGTTT
    GCTATTCGGG GAATGCTGAA GACATTTGGT CTTCATGGTG TTGTCCTGGA TGTTGATTCA
    GTGAATGAAT TGGTACAAGT AGAGACCTAT CTCAGAAGTG AGGGAGTTCT GGTAAGATAT
    TGGTATCCTA TTGACATGTT AGAAAGGCCA CCTGCTGGTT ATAGGAGGAC TGCAACAAAT
    GGATTGGTTA CCCTGGACAA TACCAATATC CAAATCCACA GAGAGCTTTT GAGAAGTGAA
    GCTGCACTGG CCAAGCTGTA CTGCCGCATG GCTCTCCTCA ATATCTTTGC CCCCAAATCT
    CCACATATGT TCACCCGTCT GTTTCATATC CCCGCTATCC GTGATATCAC TCTGGAGCAC
    CTGCAGTTGC TATCCAACCA ACTTCTCGCA CCACCTCTTC CTGATGGAAC AATCAGTTCA
    AGTTCCATTC TTTTGGCTCA GTCACTGCAA CACTGCATCC ATTCCCAGAA CTGTGCTGCC
    ACAGACCTCT TCTACCAGGG CAATTCACAA GCCATCAGAG AGTGGCTTAC TGTTGCCATC
    ACTCGGACAC TGCATCAAGG AGAGGAGAGC CTGTTAGACC TCACTAAGCA GATCTGCTCT
    TTTTTGCAGA CTGCACCAGA ACAGTTTCCT GCTGAAGAAT TCCCAATTTC AGAATCCAAA
    GTCAACATGG ATGTTAATTT TCCTGGGGCT GCGTTTGTAA TTGTGTCCTG TAAAGAAAGC
    CAGTCTGGAT TCCGCAAAGA TTCTTCCCTG TATAAAGCTC CTTGGGCTCG GGTGCTTGTG
    TACGGGCTGG GGCACAAAGT TAAGAGGAAT GGCCAGTTAA ACCTGATTGA GGCAGTGTGT
    TACCCTCGAG ACGCCTCTCC GACCAACACA GGCCTAACAC CTCCCCCAAC AACCAATCAG
    TACCCTTCAG TGATCACTCC TACAGACAAA GTGCACATCA AACTAGGAGT GTCTCCTCCT
    CCTGGAGCTG TGCTGGTCTT GCATTCTCTT CCCCTTGAGT TCCCCCTGGC AATGGCATTT
    ACAGAACAGC TGTTAACTTG GAAACTGGAA GATGTGGATG GGAAATCAGA AGATGAACTG
    GATACTATTC CTCCTTCAGT TCTTTTGCAA GTAGTGGAAT TTTTAGGGAA CTTTCTGTGG
    ACAACTGACA TGGCAGCATC TGTAAAGGAA TTGATATTTC ATCTCTTGGC TGAATTGCTT
    CGCAAAATTC ACAACCTGGA GCAGAAGAAA AGTCCTGCAG GATTATCATC TTCTATTGCC
    CTTCAGCTGA ATCCCTGTCT AGCTATGCTT ATGGCCCTGC AGTCAGAACT CCATAAGCTG
    TACGATGAGG AAACACAAAA CTGGGTGTCT GGAAATGCAT GCGGTGGTTC TGGTGTTGGA
    GCTGCTGACC AAGGAAGATT TTCAACATAC TTCCATGCTC TGATGGAAGT TTGCCTTGCT
    GTAGCAGAAG TAACTCTACC TATCAATATG AGCGTAACAG CTAATGTGAT ATCTTCAACG
    AGTGCACCAA ATCTTAGTGA CTCTTCCTCC TCATCTTCAT CTTCTCCAGG ACAGACACCG
    CAGAGTCCAA GCTTATTGTC AAAGAGGAAA AAAGTCAAAA TGAAGAGAGA AAAGACATCG
    TCTTCAGGCA AACGATCTTC ATCCAGAGCA GCTGAAACGG ATGCTGCAAT CCTCAGTATA
    GGAGGAAGCA AGCCTGAGGA TATGCTATGG TTTCATAGAG CACTGACTTT GCTTATCATT
    CTCAGACACC TAACGAAAAA GGATCCGCAA GGGTTGGGTG TGACAAACGA TGCTGTAGCA
    GATGCATGTC AGGCACTGGT AGGACCCACA GCTCATAGCC GTTTGCTTGT CATTTCTGGA
    ATACCAACTC ACTTGGAGGA GAGTACTGTC AGAAATGCAA TCAGGAAGGC GTGTAATGCG
    CATGGTGGAG TCTTCAAGGA TGAAATCTAC ATCCCTCTGC AGGAAGAGGA TCCCAAAAAA
    ATCAAGGACA AAGCAGAAGG AGGAGAATGC AGAACTGAGC TGGAAAAGCC AACTGTTTCA
    AGTAGTGCGG ACAGTCTGGA TATCAGCAGC TCTAGCAGTG TCACACCTGC TATGAGCGTT
    AGTGCGTCAG CATCAACGAG CCAGGCTTCC CTGTGCAGCT CACAGGGTAT CTCCCGGACT
    GTTAGTGACA TCTCTGTTGA TCAGTACCAG GCTGGGCTGG AACTGGCCAT CCCACCAGGA
    CTGCTGGAGC CACACGTTTC CAGCCAGGAG AGCTTGGATC TGTCGCACTG CAGTACTGGG
    AGCCTTGGCA GCCTTGGCAG CCTCGGGGAG CCGCTTGACA ATGTAGAGAC AGCATCAGTG
    TCTGATGTCG GGTCGATGTA CACGGTCACA TCTCTGGACA ACCAGCAACT TCTGTCACGG
    CCTATCAAAG GTTTTGCTGT GGTGGAGATA CGATCAAGAG CTAAAATAGA GAAAATCCGA
    GCCAGCCTAT TTAACAGTAG TGATCTCATT GGTTTGTCAA GTCTGGATGG TGAAGACGAA
    CTGATGGAAA TGTCAACTGA AGAGATTTTA ACTGTTTCTA CTGTAAATCA GAGTTTATTT
    GACACACAAG GGAGCCCGGC GCTAGAAGAC TACTTCAATG ATAAGTCAAT AAAAGGTGAG
    AAATTGGTTC CTGGTGCTCG GGAGGTCCTA ACAGAAATAT TTAAAAGCTG TGTCCATTCT
    GAGCAAATGC TGAGCCTGAC TCCAACAAAA CCAATCAAGG TTGCTGATAT CTATCTTAGT
    AAAGAGCAGA TAAATTCTCA GACTCCTGGA AACCTTCTTC ATGTCTTCTT CACAAATGTC
    CGTCCTCCAA AGAAGGTGCT GGAGGACCAG CTTACTCAGA TTCTAAGGAA ATACGGTGTG
    CCCAAGCCGA AATTTGACAA GAGCAAATAC AACAAGGCAG GCAAAGAACA GCATCCGGTG
    AAGGTTGTGA GCACCAAGCG CCCAGTTACA AAACCACCCA CAAAAGAAAA GTCTGTACTC
    AACAGCGTCA GCCGAACTGC TTTGAGTGAG AAGAAACCTA CTGTAAAGCC AAAGTCTCCT
    GAGAAGAGCA AACCTGAAGA GAAGGACCCT GAAAAGTCTC CCACAAAGAA ACAAGAAGTT
    CCTGAGGAGA AGTACCTGAC ACTGGATGGA TTTCACAGAT TTGTCATTGA CCGATCTAAG
    CAGGATATCC GTAGTGTGTG GAGAGCTATT CTGTCCTGTG GATATGATCT GCATTTTGAA
    AGATGTGCCT GCATTGATGC AAGACATGCA CAGAAAGCAT CTCGGAAGTG GACTCTGGAA
    ATGGACGTGG CTCTCGTTCA GTACATTAAC AGGCTTTGTC GTCACCTCGC AATTACACCA
    GCACGACTCC ATCCACATGA AGTATATTTG GATCCAGCTG ATGCAGCAGA TCCTAGAATC
    TCCTGTCTGT TGAATGTTCC CGTTGAAAGT CTCCGCCTGC GGTTTGCACT GCTTCAGTCT
    CTCAATACGA CGTTAGAGAC GTTTTTCTTA CCGCTGGTAG AGCTTCGGCA GACTGAGATG
    TACAGTAACA GCATTGCTGC GCTGCTGCAG GAGGCCAAAG GTTTAATCTT CTATGACACA
    AAAATAACCG TAATGAACAG AGTGCTGAAT GCCACTGTCC AAAGAACTGC TGATCACGCA
    GCCCCAGAAA TCACCTTGGA TCCCTTGGAA ATAGTTGGAG GGGAGATCCG TTCTTCAGAA
    AATTCCTACT TCTGCCAGGC AGCAAGGCAG CTGGCCTGTG TGCCATCCTC ACAGCTCTGC
    GTCAAACTCG CCAGTGGTGG GGACCCTACG TACGCGTTCA ACATCCGGTT CACAGGGGAG
    GAGGTCCATG GAACAAGTGG ATCTTTCCGT CACTTCCTCT GGCAAGTTTG TAAGGAGCTG
    CAGAGCTCCT CGCTCTCTCT TCTCCTGTTG TGTCCAAGCT CTGCGGTCAA CAAGAACAAG
    GGGAAGTACA TTTTGACACC TAGCCCCATA ACCTATGCAG AGGAGCAGCT GTTCCATTTC
    TTTGGGCAGC TTCTGGGCAT TGCAATTCGA GCAGATGTTC CCCTGCCACT CGACCTCCTG
    CCCTCATTCT GGAAGACTCT GGTAGGAGAG CCGCTGGATC CTGATGTTGA TCTCCAGGAA
    GCTGATATCC TCACCTACAA CTACATTAAA AAGTTTGAAA ATATAAATGA TGAAACTGAA
    CTGGAAGCTC TGTGTGCAGA AATTGCTTCT CAGCATTTAG CAACAGAGAG CCCTGACTGT
    CCTAATAAGC CATGCTGCAA ATTCACCTAC CTGACCATGA CAGGGGAAGA GGTGGAACTC
    TGTCCCAGGG GCAGGCACGT CCCAGTGGGA TGGGAGAACA AGGACGTGTA TGCAGCTGCA
    ATCAGGAGCT TGAGAATGCG TGAGCTGCAG ACTCCAGAGT GCATGACTGC AGTGCGTGCT
    GGGCTTGGGT CCATCATTCC CCTGCAGCTT CTGACCACGC TGACCCCTCT TGAGATGGAG
    CTGAGGACAT GTGGGCTTCC ATACATCAAC CTGGAGTTTC TCAAGGCGCA CACGATGTAC
    CAGGTGGGAC TGATGGAAAC TGATCAGCAC ATTGAGTTTT TTTGGAGCGC GCTGGAGATG
    TTCACTCAGG AGGAGCTCTG CAAGTTCATA AAGTTTGCCT GTAACCAGGA GCGGATCCCC
    TTCACGTGTC CTTGCAAGGA CGGCGGTCCC GATACCGCCC ACGTCCCGCC TTACCCCATG
    AAAATAGCCC CCCCTGATGG AGCTGCAGGT TCTCCGGACT CTCGGTACAT CCGTGTGGAA
    ACGTGCATGT TCATGATCAA ACTTCCTCAG TACTCATCAC TGGACATCAT GCTGGAGAAG
    CTCCGATACG CCATCCACTA CCGGGAGGAT CCTCTCAGCG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015131030.1
    CDS263..13507
    Translation

    Target ORF information:

    RefSeq Version XM_015275544.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015275544.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCCC CGGCGGCCGC TTCGGCGGCG GCGACGGCGG CCAGCGACTC GGCGCAGTGG 
    CTGTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGATCTG
    GCCGAGCTGC CCAGCGAGAT CGTCGGAGTG GCGGAGCCCG GGGACACGGA GCTGGAGATA
    CTTACGTTTG AGACCAAGAA CCCGACAGAG CTAGCTGAGC GCTTACGGTC TGTGTGTGGA
    AACCAGAGCA ATGCTTATGC TCGACTGCTG GAGTACCGCC TGAACGCTTT GCGAGGACTC
    TGGAACGCCC AGCGGCAGCT AGCATTGGAG GAGCAGCATG ACCGGGAGAG CTCAGGGGAT
    GAAGAAGCAC TGGCCTTACT CAAACGTCAA GGCTTGTTAC AGCAGCCTGA GCAGGCGCCC
    TTTACTTCAC GAATGGGGCT TCTGCTGGTT TTTCCCCTCA TTCAGTCTCA AAGTAGAACA
    GACCCTTCTC TCTGTAACAT AACCGCTGAG GTGCTATTGA ATTGCCTTCG AGACTGCCAG
    CCTTTGAGCT TGACTAAGGA GCCAGCTGAC TGTCTCAATG GGATAGAGTC TTTGCTGTGC
    TCGTGGTTGG AAGAAACTTC TGCTTCAGGT CATCAGATTC CATATAAGCA AAAGGAAAAT
    GCTGCTGCTG CCCTAGTTGC CTTGGCTTGT GCAAGAGGGT CGCTGAAAAC CTTTGTTCAT
    ACAGTGCATC TATTACAGAA ACAGACAGAT CTGGGAACCT TGCCTGTGGC TGATGTGTTA
    TACAGGTTGT TGCTTTTGGA GGGAGGTCCT GGCTCACCTT CTTGTTTGCT TGGAGGAAAG
    CATATAGTGT CCTGGGGCTT TGAAGACATG TTACCTGCAC CTGATGCCAA CAGTGGCTCC
    AGTGGTGAAA ATAAAGATGC AGACTTAGGA CGTTGTCTTG CAGCAGATGG CCTCTACCTT
    TATACAACAA ATTCAGTGGG AAGAGGAATA AGCAAATTAG GATCTGGATT ACATGGTACC
    TTGCGAGGTT TTGTCTACTG CCGAAATGAG GAGCTGGAGG CTGGGTGGGT TGCTTTTGGC
    AACAGCAAAC TTCTTCATCG GCCTGTCTCT TTTGATAATA AGCCTCACTC CCTTTTCCAG
    GTTGTGGATC AGCATACCCT TCAGGTGTGT CAAATAATCC CAATGCCAGT AAATCACTTC
    CCAGTTGGCA GTACTATGAC CACCGTGCAT CTTTCTTCAG ATGGTACATA TTTCTACTGG
    ATTTGGTCTC CTGCAAGCCT CAATGAGAAA ACACCAAAAG GCCACTCTGT CTTTATGGAT
    GTTTTTGAAC TTATAGTAGA AAATGGTGTG TGTATAGCAA ATCCTCTACA GGAACGAATT
    ATATTAATGC GAAAGGAAGG TGAATCTGCA AAGAGTATTA ATGAGATGCT TCTGAGCAGG
    CTGTCCAGGT ACAGAGCCTC CCCATCAGCA ACATTGGCTG CTCTCACTGG CTCTACCATC
    TCTAATACTC TAAAAGAGGA CCAAGCAGCT GCTAACACAA GCTGCGGGCT GCCACTGAAA
    ATGTTAAGGA AGACTCCGAT ATACACATGT GGAACATACC TGGTGATGTT GGTCCCACCC
    CCAGGAGGGT CAGGCAGTTC TGCAACACGC TCACTCTTTG GAGGAACAAG TGCTTTGTCA
    TCTTTAAAAA TTCTTGCTTC AAGCTTGGTG TTCAATATTT CGGATGGGCA GTTCACAAGC
    CGAGCAGATC TCATTGATGC TGCAGGAAGT TCACTTGGAC GTGGTGCTCT GGTACCAGGA
    CTTGGTGCCT GTTATGATAC TATGAATAAC ATGATATGGA CGTGCTCTAA TGATTACATC
    GATCAGTGGT GTAATCCTGG GAACCAGGCA TTCCACTGTG TCTGCCAGAG GCTGGGAGTG
    AGCCATGTCA TTACAGAGCC CAAAGGGGAT GCAACAACCA CAAATGAGGT TATTAACCAG
    TTACTGCACC ATGTTGGTGC CATGTGTATC CACCAACTCA ATCTGCTTGC AGCCAGCAAT
    AATCTTCCCA TAACAAACTT CTTGGGGAAG CAGCATCCAA TCGAAGCACA TCATCTCAGC
    AGTATCTGTG ACATTATGGA GAAAGCTATG GTGAACGGAG ATACTTGTAT AATACGCTGC
    ATCCTTGTTG TCTTCCAGGT GGTTTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGAAAC
    AGAGACATTG TGAGGAGGTC GGGCCTATTG CTTTGGCAGT TGTTGATGGC ACCAAGGGAC
    CAGATTTGTG CGGAGATTCA GAAGGAGGTT TGCCTGGCTA TAAGCTCTGG TTTAAATATC
    CTGTATCCTG GAGAAGCAGA AATCAATAAT TTATTGAAAT TGGTCTTAAC TGAAGGAGAG
    AGAAATAGTG GTCTTTCTCA ACTTCGGGAC GTCATCCTAA CAAATCTAGC AGAACAGCTT
    CAGAACAACA GATTTGGGAG TGAAGATGAT GATCATTATA GACTAAATGA TGAGCTGTTG
    CACTACATTC TCAAGATTGT TGTCCGAGAA TCTTGTGTCT TAATCACCAA GTGCCAAACT
    GTATCTAAAG ATGATTTTCA AAGGCTTCTT TCAACTGTGC CTGCTGCATC TTCATGCTTG
    CGATATCTGA TGGCCGTACA GAACCACCTC CTCAGTAACA CTATTTTGAT TAAACCTGAT
    GAAAATGATG ACAGTGACAG CTCCTTACAG GGAGAGACAT TGAAGGTACA GGAGCTGAAG
    ACCAGCGTTT TGTCTCTTGC TACACAAATT CTGACAGGAT GTGATGAAGT CTTAGAAATG
    CTGCAGCAAG TCACTACAGC ACTAATAAAC AGTGATATTG CTGACAGAGA ACAAAGATTA
    AAAGGCCTGG AACAAATTAC GAAAGCTACT ATGCTTGGTC ATCTGCTTCC TGTCCTTCTG
    ACATCCCTGA TGCATCCAAA TTTGCAAACT CTAACCATGG CTGATGCCTT GATGCCTCAG
    CTGGTGCAGC TGGTTCTCTA TACAAGTCAG ACTGCACTGC TGCTTAAAAC TCAGTCTCCG
    GTTTTCTCAG AACTGAACAG CTCTCCTGGT GGTGTGTCGG AACACAAAGG GAAATTGTTA
    CATGATGAGA GAATGCTGGA GGAGAAGGAA GAGCCAGGTT TTCTTACAGG CTTGAAGATC
    CCTGCTCCTT GGGCTGCTGG GAAGACTGTA GAAACTGTTC ATCCTGTCAG GGATAACTAT
    AAATTTAAGG AAACTGTACA CATACCAGGA GCCCGCTGTT TGTATCTTAG ATTTGACAAC
    AGATGTTCTT CACAATATGA TTACGATAAG TCTAACAGTG TATTTTATGT TTTGAAGTTG
    GTGATCTATG CTGGTCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT AGTAAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGGAGTG GTCGTGAGCA CAACACTCCA
    GACAAAGCCA TGTGGGGCTT TGCTTGCACT GTTCGAGCAC AGGAGTCTTC AGAGGATGTG
    TCAGGAGGCC TGCCATTCTT AGTTGACCTG GCACTTGGCC TTTCTGTGTT AGCTTGTTCT
    ATGCTTCGGA TTTTATACAA TGGACCAGAA ATTACCAAAG ATGAAGAAAC CTGTCAAGAG
    CTTCTAAGAT CTAAACTATT ACAAAGGTGC CAATGGCAGG TGGAAGCTAA TGGTGTAATA
    TCTCCAGCCC TTACACCGAG TCCTTCTCCA CTGCCTCTTA CTATAGATGA GGACAGAGAA
    TTTACTTATC CTTCTGATGT GCTGGTGCCT CCCATTGGAC ACTATTTTGA TCTGCCTAGG
    ATTAGGCTGC CTCCAGGAAT CATGATAAAA CTCAGGGAAA TTTCTGGACG TGCTCGGCCT
    CAATTTAGAC CTAGCATAAA GGAAGTGATT CAGCCCGAGG TAATGGAGGA GATGGTAGTT
    TCATGTGTGA TTAAACATTT GAATTTGGTT GATGCACTCC AGTCCCTGAT AAACTTCCAG
    TATCAGGAAG AACATGCTGA AGAATATGAT TTACTTTGTA AAATTATGGG AGAGACTTTT
    AAGAAGCTAA ATGCCATGGA GAGACAATTA CAGAGTGTGG CTGAGCTGGA ACAGAAATGG
    CAGAGTGAGG TTGATGACGC CATGCATGGG AAGCTGGAGA ATAATGTTCC CTTCTTCTAT
    GATTACCACT TCAATGAGAG CAAGATGAAA GAACTGGAAC TTCTGTGTTC TATGAAGGAA
    GTTTCTTTTG ATGGAAGTGA TCTTGAGAAT GTGGTCCTGG CATTAAGGGA GAAGTTCTTT
    CAAGAGGTGA ATTCACTGAT ACAGAAGACT TATCATCCCC TAGCGAAAAC AAAAGCATTA
    GTTAAAAGCT TGATGAACAG GGCAGAGCTG TTGCTGCATG TCACTATTGC AGCGCAGTCT
    GGTATCACAA GAAGTATATC TGGAACCCCT GCAGAGACTC CAGTGCGCCA GCCCGTCTTC
    CTTCGCAGTA TGTCAGCACC ATCTGACTTA GAGATGATTG GTAACGAAGA TATTGAGTTT
    GCTCGATCAA GTCAAAGGCG ACGCCATGTT ACCAGCCACA GAAGTAGCTC TTTCACTCTT
    CTGCAATCAC TGACCATTGA GGACAGCAGA GATAAACCTA CATACAGTGT CCTACTGGGG
    CAGCTCTTTG CTTTCATTGG AACTAATCCT GACCAAGCGG TATCCAGCAG CAGTTTTCTG
    CTCGCTGCTC AGACAAGGTG GCGGCGTGGG AACACAAGGA AGCAAGCCTT AGTGCATATG
    AGAGAGCTGC TGACTGCGGC TGTTCGGGTT GGTGGGGTGA CACACCTCGT GGGCCCTGTG
    ACCATGGTAC TTCAGGGAGG ACCAAGAATT GAAGAGCTCA CGTGTGGTGG GATGGTGGAA
    CAAGTCCAGG AAGCTTTTGG GGAGACTATG ACATCCGTGG TCTCGCTGTG TGCTCGCTAC
    CCCATTGCTT GTGCAAACAG CATTGGCCTC TTATGCACTA TACCTTACAC AAGGAGTGAA
    GAGAAGTGTT TGGTACGTAG TGGCCTGGTA CAGCTTATGG ACAGGCTGTG CAGCTTAAGC
    AGCCAGGCAG AATCCAGTTC AAATGAAAAA CAGACCAAGA AGCAGAAGGT GGCCACCATG
    GCTTGGGCTG CCTTTCAGGT GCTGGCCAAT CGCTGTGTTG AATGGGAGAA AGAAGAAGGT
    GGTTCAACAG AAGCTGTTCA CTCTGGTCTG GCTCGTCAGG TCTCCAGCCT TCTTACCAAT
    CACCTTGCAC GAGCTACAGA GTGTTGTGGT AATCAAGCTG CAGGAAATGA TGCACTTCAA
    GACGTCCTCA GTCTTCTTAA TGACCTGTCG AGGAGTCACA TAGGTAAAGC AATCCTGAGC
    CAGCCAGCCT GTGTGTCCAA ACTTCTGTCA CTTCTCTTGG ATCAACGTCC CTCTCCAAAG
    TTGGTTCTTA TAATCCTTCA GTTGTGTCGT GCTGCTCTAC CTCTCATGAG TGTTGAAGAC
    TGTGGAAATG TAGAACTGCC TCCCTGGAGT TACTCTGTGC CCTCCCTGAA CAGTGAACAA
    GATGATCCCA GCGACCCAGC CTCCAAGATT GCATCTCTGC TCTTAGCAAA GCTGGCAGAT
    TATGTAGTAC CAGGATGCCA GACAGTTCTT TCTCCAACTG CTTCTGAACC AGATACTGCT
    TTAGCAAAAA GCAGCCCTAA GAATTCTATA AAAGGCGATA AAGACCCTGG AGAAGAAAGT
    GAGGCAGTGG ATGGCAAACT TTCTATTTTC ATCCATAAAC GGGAAGACCA GTCATCCCAC
    GAAGTGCTTC AGCCATTGCT GAGTAGCTCC GAAGGTCGCC CATTCCGACT AGGAACTGGA
    GCCAACATGG AGAAGGTGGT GAAAATGGAT CGTGACATGA CTAAAAGTGG CTGCTGTGAA
    GTTATCACAG AAGAAGCTGC TGCAGCTCTT CGCAAGGCAA CTAAGTGGGC TCAGTCTGGT
    CTGATAGTTA GTGTTGGGCC ACCCATAGAA ACTGTCAATC CAGAAGCAAC CAGTGGGATG
    TCCTCTGGGG ACAAAAAGAA GACTGCACAG ACATCTATTT GCAGAGAGAG AAATTCTGAG
    CTTGCCAGGA CTGATCCTGT AAGACCATTC ATCAGTGGTC ATGTTGCAAA TAGCATGGCA
    GCAGAAGTGA TCGCTTTGCT ACATAGCTTG TTGATGGCAC CAGAATCAAA TGCGGCTCAA
    ATATGGACAA CAACTGCTGA AAAAGTTCTG TCTCGGGCTC TGATGTACAT TCCACAACTC
    GGGAAATACG CAGAGAGTAT TTTGGAGAAT GGAAGCAGCA GTGGAAGGAA GCTTGCTAAA
    CTTCAAAGAA TTGCTCGACA GGCAGTAGCT GCTCTTTGTG CTCTAGGTGG CTTTAAGGAG
    ACAATTAAAA TCGGTTCTGA AGTTCAGGTT TTAGGTAAAG GAATAGCAGG AAGCATTGGA
    GTTGTGGCAT CTATTAATGA ACAAGAAGGT ATAGCAACGG TCAAATTTCC GCCTACCACT
    ATAGACAGTA GAAAGACCTC ACAAGCATCA GACACTTTAA CTATTCCGTT ATCTAGGCTC
    TGTGTTCCAA GATCTGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TATCTATTCA CACCTCACTT
    CCTGCAACAG GAGATGGCTC AACTCCAGTA ATGGCAGTGG TCCGGCTCCT TGCTGAAATA
    AGAACCAGAG CATGCCTGGT GATGGCTCAG CTGTTAGAAG ACAGTTCATT CTGTGAAGAA
    TTCATTCAAC AGTGCCCAGC TGCTGTAGAG GTTCTTAATT TGGTAGCCCA GGAGTGCAGC
    CCTGGAGAGA GGCTCACTGT AGTAGAAATG CAGTGTGAAA GGTTAAGGAT GTTGTATCGT
    GACTGTGCTC GACCCCCACC TCCTCCACTG CAGGCAGACA GAAGGCAGCC CAAAGAAATC
    ACTTGGAGCC CATCAAGAGT GTTTCCCCCA GTACGAGCAT GCATGTTTTC ATCACATCTT
    GCTGCTGTGA CCTTCCTGGC TGATCCAAGT GCAGGTGGGG GACTTCCTCG GGGAACGTTC
    ATTTATGCCA CTTCTCCAGT TCCCGTGCAG GCACCATCAT TCTACTGGGA AATTGAAATA
    GTTTCCTATG GAGATACAGA TGATGACACT GGTCCAATAG TTTCATTTGG ATTCGCTACA
    GAGGCAGAAA AACGGGATGG GGCTTGGACA AACCCAGTGG GCACCTGTCT TTTTCATAAC
    AATGGGCGAG CAGTGCATTA CAATGGCTCC AGCTTGTTAC AGTGGAAGAG TGTTCGATTG
    GATGTGACTC TTTCTCCTGG TGATGTGGCT GGAATTGGAT GGGAAAGAGC AGAAGGAACT
    CCACCCCCAC CAGGGCAGCC TGCTAAAGGC AGAGTTTATT TTACTTATTG CGGGCAGCGA
    CTTGGGCCAT ACCTAGAGGA TGTCTCTGGT GGAATGTGGC CAATTGTTCA CATACAGAAA
    AAGAACACAA AAGTCCGTGC TAATTTTGGA TCTCGCCAGT TTGCCTATGC CGAAGGCCAA
    GCTCACAGGA ATGCTGCTGA TCTGTGTATT GACCTAGCTG AAGAGATAAG TGCCAACTTT
    GAGGCATTGC CATTTGCTAT GGCTTCTGAT AGCGATAACG ACGCTGGCAC CAGCATAGCT
    TCTGATCCTG GGACTCACGG ACCTCCCTGT AGAATTGCTG CTGTTGCTAC GGCTCAACAA
    CAATATGACA GTGACACATC TTGTCACTAT AAAATGGAAC TAAGCTATGA GAACTTCATC
    ACATCAGGGC CTGATCCTCA TCCCCCTCCT ATTGCAGATG ATGAAAGTGA TGATGACGAC
    GATGATGATA TTCCCAGGGA GGACCACTAT GCCCTGCTGG TTAAAGCCTG GGAAACTAAA
    GTTTTCCCTA CAATTAGAAG AAGATTCCGT AATGAGGCTG AGAGGAAATC TGGGCTGGAT
    CAAATTAAAG GAGCCTTACA GTTAGGAATG GTTGATATAG CACGACAAAC TGTAGAATTC
    TTGTATGAGG AAAACGGTGG CATCCCGAGA GACCTCTACC TTCCTACCAT TGAGGATATC
    AAAGATGAAG CAAACAAGTT TACAATCGAT AAAGTACGAA AAGGTCTCAC TGTTGTAACT
    CGCTCTCCTG ACAGTAACAA CGTTACCAGC AGTGCTGTTG GAACAGCTTT ACCCAAGTTT
    GCTATTCGGG GAATGCTGAA GACATTTGGT CTTCATGGTG TTGTCCTGGA TGTTGATTCA
    GTGAATGAAT TGGTACAAGT AGAGACCTAT CTCAGAAGTG AGGGAGTTCT GGTAAGATAT
    TGGTATCCTA TTGACATGTT AGAAAGGCCA CCTGCTGGTT ATAGGAGGAC TGCAACAAAT
    GGATTGGTTA CCCTGGACAA TACCAATATC CAAATCCACA GAGAGCTTTT GAGAAGTGAA
    GCTGCACTGG CCAAGCTGTA CTGCCGCATG GCTCTCCTCA ATATCTTTGC CCCCAAATCT
    CCACATATGT TCACCCGTCT GTTTCATATC CCCGCTATCC GTGATATCAC TCTGGAGCAC
    CTGCAGTTGC TATCCAACCA ACTTCTCGCA CCACCTCTTC CTGATGGAAC AATCAGTTCA
    AGTTCCATTC TTTTGGCTCA GTCACTGCAA CACTGCATCC ATTCCCAGAA CTGTGCTGCC
    ACAGACCTCT TCTACCAGGG CAATTCACAA GCCATCAGAG AGTGGCTTAC TGTTGCCATC
    ACTCGGACAC TGCATCAAGG AGAGGAGAGC CTGTTAGACC TCACTAAGCA GATCTGCTCT
    TTTTTGCAGA CTGCACCAGA ACAGTTTCCT GCTGAAGAAT TCCCAATTTC AGAATCCAAA
    GTCAACATGG ATGTTAATTT TCCTGGGGCT GCGTTTGTAA TTGTGTCCTG TAAAGAAAGC
    CAGTCTGGAT TCCGCAAAGA TTCTTCCCTG TATAAAGCTC CTTGGGCTCG GGTGCTTGTG
    TACGGGCTGG GGCACAAAGT TAAGAGGAAT GGCCAGTTAA ACCTGATTGA GGCAGTGTGT
    TACCCTCGAG ACGCCTCTCC GACCAACACA GGCCTAACAC CTCCCCCAAC AACCAATCAG
    TACCCTTCAG TGATCACTCC TACAGACAAA GTGCACATCA AACTAGGAGT GTCTCCTCCT
    CCTGGAGCTG TGCTGGTCTT GCATTCTCTT CCCCTTGAGT TCCCCCTGGC AATGGCATTT
    ACAGAACAGC TGTTAACTTG GAAACTGGAA GATGTGGATG GGAAATCAGA AGATGAACTG
    GATACTATTC CTCCTTCAGT TCTTTTGCAA GTAGTGGAAT TTTTAGGGAA CTTTCTGTGG
    ACAACTGACA TGGCAGCATC TGTAAAGGAA TTGATATTTC ATCTCTTGGC TGAATTGCTT
    CGCAAAATTC ACAACCTGGA GCAGAAGAAA AGTCCTGCAG GATTATCATC TTCTATTGCC
    CTTCAGCTGA ATCCCTGTCT AGCTATGCTT ATGGCCCTGC AGTCAGAACT CCATAAGCTG
    TACGATGAGG AAACACAAAA CTGGGTGTCT GGAAATGCAT GCGGTGGTTC TGGTGTTGGA
    GCTGCTGACC AAGGAAGATT TTCAACATAC TTCCATGCTC TGATGGAAGT TTGCCTTGCT
    GTAGCAGAAG TAACTCTACC TATCAATATG AGCGTAACAG CTAATGTGAT ATCTTCAACG
    AGTGCACCAA ATCTTAGTGA CTCTTCCTCC TCATCTTCAT CTTCTCCAGG ACAGACACCG
    CAGAGTCCAA GCTTATTGTC AAAGAGGAAA AAAGTCAAAA TGAAGAGAGA AAAGACATCG
    TCTTCAGGCA AACGATCTTC ATCCAGAGCA GCTGAAACGG ATGCTGCAAT CCTCAGTATA
    GGAGGAAGCA AGCCTGAGGA TATGCTATGG TTTCATAGAG CACTGACTTT GCTTATCATT
    CTCAGACACC TAACGAAAAA GGATCCGCAA GGGTTGGGTG TGACAAACGA TGCTGTAGCA
    GATGCATGTC AGGCACTGGT AGGACCCACA GCTCATAGCC GTTTGCTTGT CATTTCTGGA
    ATACCAACTC ACTTGGAGGA GAGTACTGTC AGAAATGCAA TCAGGAAGGC GTGTAATGCG
    CATGGTGGAG TCTTCAAGGA TGAAATCTAC ATCCCTCTGC AGGAAGAGGA TCCCAAAAAA
    ATCAAGGACA AAGCAGAAGG AGGAGAATGC AGAACTGAGC TGGAAAAGCC AACTGTTTCA
    AGTAGTGCGG ACAGTCTGGA TATCAGCAGC TCTAGCAGTG TCACACCTGC TATGAGCGTT
    AGTGCGTCAG CATCAACGAG CCAGGCTTCC CTGTGCAGCT CACAGGGTAT CTCCCGGACT
    GTTAGTGACA TCTCTGTTGA TCAGTACCAG GCTGGGCTGG AACTGGCCAT CCCACCAGGA
    CTGCTGGAGC CACACGTTTC CAGCCAGGAG AGCTTGGATC TGTCGCACTG CAGTACTGGG
    AGCCTTGGCA GCCTTGGCAG CCTCGGGGAG CCGCTTGACA ATGTAGAGAC AGCATCAGTG
    TCTGATGTCG GGTCGATGTA CACGGTCACA TCTCTGGACA ACCAGCAACT TCTGTCACGG
    CCTATCAAAG GTTTTGCTGT GGTGGAGATA CGATCAAGAG CTAAAATAGA GAAAATCCGA
    GCCAGCCTAT TTAACAGTAG TGATCTCATT GGTTTGTCAA GTCTGGATGG TGAAGACGAA
    CTGATGGAAA TGTCAACTGA AGAGATTTTA ACTGTTTCTA CTGTAAATCA GAGTTTATTT
    GACACACAAG GGAGCCCGGC GCTAGAAGAC TACTTCAATG ATAAGTCAAT AAAAGGTGAG
    AAATTGGTTC CTGGTGCTCG GGAGGTCCTA ACAGAAATAT TTAAAAGCTG TGTCCATTCT
    GAGCAAATGC TGAGCCTGAC TCCAACAAAA CCAATCAAGG TTGCTGATAT CTATCTTAGT
    AAAGAGCAGA TAAATTCTCA GACTCCTGGA AACCTTCTTC ATGTCTTCTT CACAAATGTC
    CGTCCTCCAA AGAAGGTGCT GGAGGACCAG CTTACTCAGA TTCTAAGGAA ATACGGTGTG
    CCCAAGCCGA AATTTGACAA GAGCAAATAC AACAAGGCAG GCAAAGAACA GCATCCGGTG
    AAGGTTGTGA GCACCAAGCG CCCAGTTACA AAACCACCCA CAAAAGAAAA GTCTGTACTC
    AACAGCGTCA GCCGAACTGC TTTGAGTGAG AAGAAACCTA CTGTAAAGCC AAAGTCTCCT
    GAGAAGAGCA AACCTGAAGA GAAGGACCCT GAAAAGTCTC CCACAAAGAA ACAAGAAGTT
    CCTGAGGAGA AGTACCTGAC ACTGGATGGA TTTCACAGAT TTGTCATTGA CCGATCTAAG
    CAGGATATCC GTAGTGTGTG GAGAGCTATT CTGTCCTGTG GATATGATCT GCATTTTGAA
    AGATGTGCCT GCATTGATGC AAGACATGCA CAGAAAGCAT CTCGGAAGTG GACTCTGGAA
    ATGGACGTGG CTCTCGTTCA GTACATTAAC AGGCTTTGTC GTCACCTCGC AATTACACCA
    GCACGACTCC ATCCACATGA AGTATATTTG GATCCAGCTG ATGCAGCAGA TCCTAGAATC
    TCCTGTCTGT TGAATGTTCC CGTTGAAAGT CTCCGCCTGC GGTTTGCACT GCTTCAGTCT
    CTCAATACGA CGTTAGAGAC GTTTTTCTTA CCGCTGGTAG AGCTTCGGCA GACTGAGATG
    TACAGTAACA GCATTGCTGC GCTGCTGCAG GAGGCCAAAG GTTTAATCTT CTATGACACA
    AAAATAACCG TAATGAACAG AGTGCTGAAT GCCACTGTCC AAAGAACTGC TGATCACGCA
    GCCCCAGAAA TCACCTTGGA TCCCTTGGAA ATAGTTGGAG GGGAGATCCG TTCTTCAGAA
    AATTCCTACT TCTGCCAGGC AGCAAGGCAG CTGGCCTGTG TGCCATCCTC ACAGCTCTGC
    GTCAAACTCG CCAGTGGTGG GGACCCTACG TACGCGTTCA ACATCCGGTT CACAGGGGAG
    GAGGTCCATG GAACAAGTGG ATCTTTCCGT CACTTCCTCT GGCAAGTTTG TAAGGAGCTG
    CAGAGCTCCT CGCTCTCTCT TCTCCTGTTG TGTCCAAGCT CTGCGGTCAA CAAGAACAAG
    GGGAAGTACA TTTTGACACC TAGCCCCATA ACCTATGCAG AGGAGCAGCT GTTCCATTTC
    TTTGGGCAGC TTCTGGGCAT TGCAATTCGA GCAGATGTTC CCCTGCCACT CGACCTCCTG
    CCCTCATTCT GGAAGACTCT GGTAGGAGAG CCGCTGGATC CTGATGTTGA TCTCCAGGAA
    GCTGATATCC TCACCTACAA CTACATTAAA AAGTTTGAAA ATATAAATGA TGAAACTGAA
    CTGGAAGCTC TGTGTGCAGA AATTGCTTCT CAGCATTTAG CAACAGAGAG CCCTGACTGT
    CCTAATAAGC CATGCTGCAA ATTCACCTAC CTGACCATGA CAGGGGAAGA GGTGGAACTC
    TGTCCCAGGG GCAGGCACGT CCCAGTGGGA TGGGAGAACA AGGACGTGTA TGCAGCTGCA
    ATCAGGAGCT TGAGAATGCG TGAGCTGCAG ACTCCAGAGT GCATGACTGC AGTGCGTGCT
    GGGCTTGGGT CCATCATTCC CCTGCAGCTT CTGACCACGC TGACCCCTCT TGAGATGGAG
    CTGAGGACAT GTGGGCTTCC ATACATCAAC CTGGAGTTTC TCAAGGCGCA CACGATGTAC
    CAGGTGGGAC TGATGGAAAC TGATCAGCAC ATTGAGTTTT TTTGGAGCGC GCTGGAGATG
    TTCACTCAGG AGGAGCTCTG CAAGTTCATA AAGTTTGCCT GTAACCAGGA GCGGATCCCC
    TTCACGTGTC CTTGCAAGGA CGGCGGTCCC GATACCGCCC ACGTCCCGCC TTACCCCATG
    AAAATAGCCC CCCCTGATGG AGCTGCAGGT TCTCCGGACT CTCGGTACAT CCGTGTGGAA
    ACGTGCATGT TCATGATCAA ACTTCCTCAG TACTCATCAC TGGACATCAT GCTGGAGAAG
    CTCCGATACG CCATCCACTA CCGGGAGGAT CCTCTCAGCG GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.