HECTD4 cDNA ORF clone, Bos taurus(cattle)

The following HECTD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa224637 XM_024977763.1
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224638 XM_015475527.2
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224639 XM_010814058.3
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224640 XM_010814059.3
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224641 XM_010814061.3
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224642 XM_015475528.2
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa224643 XM_024977764.1
Latest version!
Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52727 XM_010823423.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52727 XM_010814061.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52729 XM_010814057.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52729 XM_015467161.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52730 XM_010814058.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52730 XM_015467163.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52731 XM_010814059.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52731 XM_015467164.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52732 XM_010814060.2
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa52732 XM_015467165.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93228 XM_015475527.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93228 XM_015467162.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93229 XM_015467166.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa93229 XM_015475528.1
Latest version!
Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa224637
    Clone ID Related Accession (Same CDS sequence) XM_024977763.1
    Accession Version XM_024977763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13284bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12781
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    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG
    CCCATCACCA AGCCGCCCAC CAAGGACAAG GCAGTGCTCA ACAGCGTCAG CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833531.1
    CDS284..13567
    Translation

    Target ORF information:

    RefSeq Version XM_024977763.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG
    CCCATCACCA AGCCGCCCAC CAAGGACAAG GCAGTGCTCA ACAGCGTCAG CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224638
    Clone ID Related Accession (Same CDS sequence) XM_015475527.2
    Accession Version XM_015475527.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13281bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
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    1801
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    1981
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    12241
    12301
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    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG
    CCCATCACCA AGCCGCCCAC CAAGGACAAG GCAGTGCTCA ACAGCGTCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331013.2
    CDS284..13564
    Translation

    Target ORF information:

    RefSeq Version XM_015475527.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475527.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGGTGAG CACCAAGCGG
    CCCATCACCA AGCCGCCCAC CAAGGACAAG GCAGTGCTCA ACAGCGTCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224639
    Clone ID Related Accession (Same CDS sequence) XM_010814058.3
    Accession Version XM_010814058.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13278bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
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    1741
    1801
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    1981
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    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATCTAACAGT ATATTTTCTG TTTTGAAGTT GGTGATATAC
    GCGGGGCCTA ACACAAACAG TAGGAAGGTT GCTGAGTATG GAGGCAATAC ACTGGGATAT
    GGCAGCCGTA GTGTCTTAGG AACTGGTTGG CCGAAAGACT TGGTGAAGGT GGAAGGAGAT
    ACAGTCACCT TCTCCTTTGA AATGAGAAGT GGCCGTGAAC ACAACACTCC CGATAAGGCT
    ATGTGGGGCT TTGCTTGCAC AGTTCGCGCT CAGGAGTCTT CGGAGGATGT GTCAGGAGGC
    TTACCCTTTC TGGTAGACCT GGCTTTAGGT CTATCTGTGT TAGCTTGTTC CATGTTAAGA
    ATCCTATACA ACGGACCAGA AATTACCAAA GAAGAAGAAA CCTGTCAGGA GCTGTTGCGG
    TCCAAACTTT TACAAAGGTG CCAGTGGCAG GTAGAGGCCA ACGGTGTGAT CTCCCCTGCC
    CTTACTCCAA GCCCATCTCC ACTGCCTCTG ACCATCGAGG AAGACAGAGA GTTCACTTAC
    CCGTCTGATG TCCTCGTGCC TCCTGTTGGA AACTACTTTG ACCTGCCCCG GATCAGGCTG
    CCTCCAGGAA TCATGATAAA GCTCAGGGAA ATTTCTGGGC GTGCTAGACC ACAGTTTAGA
    CCAAGTATAA AAGAAGTAAT TCAGCCAGAT GTGATGGAGG AAATGGTGGT ATCATGTGTT
    ATTAAGCACT TGAACTTAGT TGATGCACTG CAGTCCCTAA TAAATTTCCA GTATCAAGAA
    GAACATGCTG AGGAATATGA TTTACTGTGT AAAATCATGG GAGAGACATT TAAGAAACTC
    AATGCCATGG AGAGACAGCT GCAGAGTGTT GCAGAATTGG AACAGAAATG GCAGAGTGAA
    GTTGATGATG CCATGCAGGG GAAACTGGAG AACAACATGC CTTTCTTCTA TGATTACCAT
    TTCAATGAGA ACAAAATGAA AGAACTAGAA CTTTTGTGTT CAATGAAGGA AGTCTCCTTT
    GATGGAAATG ATCTTGAAAA CATGGTGCTA TCTCTGAGGG AGAAGTTCCT ACAAGAAGTG
    AATTCTCTTA TTCAGAAACC CTCGCACCCA CTGGCTAAAA CGAAGACGTT AGTGAAGAGT
    CTAATGAACC GAGCTGAGCT GTTGCTGCAC GTCACCATTG CAGCCCAGTC TGGCCTCACG
    AGAAGCATCT CAGGGACCCC CGCGGAAACA CCGGCTTGTA AATCAGCCTC TGAAACAAAG
    GTGATCTCTC ATGCTGTGCG TCAGCCTGTT TTCCTTCGCA GCATGTCGGC TCCCTCTGAC
    CTGGAAATGA TCGGTAATGA AGATTTGGAA TTTACTAGAG CAAATCAGAG GCGGCGCCAC
    GTGACCAGCC ACCGCAGCAG CTCCTTCACA CTCCTGCAGT CATTGGCCAT AGAGGACAGC
    AGGGACAAGC CCACCTACAG CGTCCTGCTG GGGCAGCTGT TTGCTTTCAT CGGCACCAAC
    CCTGACCAAG CTGTGTCCAG CAGCAGCTTT CTTCTGGCCG CACAGACGAG ATGGCGGCGG
    GGAAACACCC GCAAGCAGGC GTTGGTGCAC ATGCGGGAGC TGCTGACGGC TGCCGTGCGA
    GTCGGGGGAG TGACACACCT TGTGGGTCCA GTGACAATGG TCCTGCAGGG AGGACCCAGA
    ATTGAAGAAC TGACCTGTGG TGGGATGGTC GAGCAGGTCC AGGAAGCCTT CGGTGAGACC
    ATGACCTCTG TGGTGTCTTT GTGCGCACGT TACCCTATCG CATGTGCAAA TAGCATCGGA
    CTCTTGTGCA CTATACCTTA TACCAGGAGT GAAGAGAAGT GCCTGGTGCG GAGTGGCCTT
    GTCCAGCTCA TGGATCGACT ATGCAGCTTG AGCAGTCAGA CTGAGTCCAG CTCCAGTGAG
    AAACAAACCA AGAAGCAGAA GGTGGCCACC ATGGCCTGGG CAGCCTTCCA GGTGCTTGCC
    AACCGCTGCG TTGAATGGGA AAAAGAAGAA GGCGGCTCCA CAGAGGCTGT GCACTCAGGT
    CTGGCCCGTC AGGTGTCCAG CCTTCTCACG AATCACCTTG CCCGAGCGAC CGAGTGCTGT
    GGCAACCAGG CTGCCGGGAA CGATGCCCTC CAGGACGTCC TGAGTCTCCT CAATGATCTC
    TCGAGGAGTC ATATAGGTAA AGCCATCCTG AGCCAGCCAG CTTGTGTGTC CAAACTCCTC
    TCCCTGCTAC TTGACCAACG CCCATCCCCA AAGCTGGTTC TTATCATTCT TCAGCTGTGC
    CGGGCGGCAC TCCCGCTGAT GAGCGTAGAA GACTGTGGGA ACGTGGAGCT CCCACCCTGG
    AGCTACTCCG TCCCCTCCCT AAACAGCGAG CAGGAGGATC CCAGCGACCC AGCGTCCAAG
    ATCGCCTCGC TGCTCTTGGC AAAGCTGGCA GACTACGTGG TCCCAGGATG TCAGACAGTC
    CTTTCTCCAA CCGCTTCCGA ACCTGACACC ACATTGACAA AAACCAGCCC CAAGAATTCC
    TTAAAAGGCG ATAAAGATCC TGGAGAAGAG AGTGAAGCAG TGGATGGCAA GCTCTCTATC
    TTCATCCACA AGCGGGAAGA CCAGTCGTCC CATGAAGTTC TGCAGCCATT ACTAAGTAGC
    TCAGAAGGAC GGCCCTTCCG ACTTGGTACT GGTGCCAACA TGGAGAAAGT TGTGAAAATG
    GACCGAGACA TGACGAAGGG TGGCTGCTGT GAGGTGATTA CGGAAGAGGC GGCAGCTGCT
    CTGCGAAAAG CGACCAAGTG GGCACAGTCA GGCCTCATTG TCAGTGTTGG GCCACCCGTG
    GAGTCCCTCA ACCCAGAGGC CGTGAGCGGG CTGTCCACAG GGGACAAGAA GAAGACTGCC
    CAAACCTCCA TTTGCCGAGA GAGAAACTCT GAACTCGCCA GGACCGACCC CGTCCGACCT
    TTCATCAGTG GGCACGTGGC AAACAGCATG GCTGCTGAGG TGATCGCCTT GCTTCATAGC
    TTGCTGATGG CACCTGAGTC AAATGCTGCT CAGATATGGA CCACGACAGC AGAGAAGGTT
    CTGTCTCGTG CACTGATGTA CATTCCACAG TTGGGGAAAT ATGCAGAAAG CATTCTGGAA
    AATGGCAGCA GCAGTGGCAG AAAGCTTGCC AAACTTCAGA GAATTGCCCG CCAGGCTGTG
    GCTGCACTCT GTGCACTTGG AGGCTTCAAA GAGACAATCA AGATCGGGTC TGAGGTCCAG
    GTTTTAGGTA GAGGAATATC AGGAAGCATC GGAGTGGTGG CTTCTATCAA CGAACAGGAA
    GGTATAGCTA CGGTCAGATT CCCACCCATA GACTGTAGAA AGACTTCGCA AGCATCAGAC
    ACACTGACTA TTCCATTGTC TAGACTTTGT GTTCCAAGAT CAGAGGCATT GCCTCTTCAT
    AAACTGTCCA TTACTGAGAA GGTAGTACAG GCAGTCCAGT CCATGTTGCT TCCTCAGGAG
    GGAAGTCTCT CTATTCATAC CTCACTTCCT GCAGCAGGAG ATGGGTCAGC TCCTGTGATG
    GCAGTCGTTC GGCTCCTGGC TGAAATAAGA ACAAGAGCAT GCCTGGTCAT GGCCCAGCTT
    TTAGAAGACA GCTTATTTTG TGAAGAATTC ATACAGCAGT GTCCAGCAGC TGTTGAAGTT
    CTTAACCTAG TAGCCCAGGA ATGCAGTGCT GGAGAGCGGC TGGCAGTTGT GGAAGTGCAG
    TGTGAACGGC TGAGGATGCT CTACCGGGAC TGTGCTCGGC CCCCGCCGCC TCCGCTGCAG
    GCTGACCGGA GACAGCCCAA AGAGATTACG TGGAGCCCCT CACGAGTGTT TCCACCTGTC
    CGAGCCTGCA TGTTCTCCTC GCACCTCACT TCTGTCACCT TCCTAGCAGA CCCCAGTGCA
    GGGGGCGGTC TGCCCAGGGG CACATTTATC TACGCCACCT CACCACTGCC CGTTCAGGCC
    CCGTCTTTCT ACTGGGAAAT TGAAATTGTT TCCTATGGAG ATACAGATGA TGATACTGGA
    CCAATAGTTT CCTTTGGCTT TGCTACAGAA GCAGAGAAAA GAGATGGGGC ATGGACTAAT
    CCAGTGGGCA CCTGTCTTTT CCATAACAAT GGCCGAGCTG TGCATTACAA TGGCTCCAGT
    TTATTACAGT GGAAGAGCGT TCGATTAGAC GTAACTCTCT CTCCTGGTGA CGTGGCAGGG
    ATTGGCTGGG AGAGAACCGA GGGGACGCCT CCTCCACCCG GGCAGCCTGC CAAGGGCCGT
    GTGTACTTCA CATACTGTGG GCAGCGCCTC TCACCGTATC TCGAAGATGT CTCGGGTGGG
    ATGTGGCCGG TGGTTCATAT TCAGAAAAAG AACACCAAGA CTCGCGCTAA TTTTGGCTCC
    CGCCCATTTG CCTACGCTGA AGGGCAAGCC CACCGCAATG CTGCCGACCT GTGCACTGAC
    CTGGCCGAGG AAATTAGCGC TAACTTCGAG GCCCTGCCCT TTGCCATGGC CTCTGACAGT
    GATAACGATG CTGGCACCAG TATTGCGTCA GACCCGGGCA CTCATGGGCC ACCATGCCGA
    ATTGCTGCCG TAGCCACTGC TCAGCAACAA TATGATAGTG ATACTTCCTG TCATTATAAA
    GTGGAGCTCA GCTATGAGAA TTTCATCACC TCTGGGCCAG ACCCTCACCC ACCCCCCATT
    GCAGATGACG AAAGTGATGA TGATGATGAC GATGACATCC CACAGGAGGA TCACTATGCC
    CTGCTGGTGA AAGCCTGGGA GACCAAGGTT TTTCCTACCA TCCGAAGACG GTTCCGCAAC
    GAAGCAGAGA GGAAATCAGG ACTGGACCAG ATAAAGGGTG CTTTACAACT AGGCATGGTG
    GATATTGCCC GGCAGACAGT TGAATTCCTC TACGAGGAGA ATGGTGGCAT CCCAAGAGAC
    CTTTATCTTC CCACCATTGA AGACATTAAA GATGAAGCGA ACAAGTTCAC AATTGATAAA
    GTTCGAAAAG GTCTCACGGT AGTAACACGC TCTCCAGACA GCAATAACGT AGCCAGCAGC
    ACTGTTGGAA CTGCCCTGCC AAAATTTGCT ATCCGAGGGA TGCTGAAAAC CTTTGGGCTT
    CATGGAGTTG TCTTAGATGT TGATTCAGTT AATGAACTAG TTCAGGTAGA AACGTACCTC
    CGAAGTGAAG GTGTGCTGGT CCGATACTGG TACCCTATTG ATATGTTGGA AAGGCCCCCC
    GCAGGCTACA GAAGGACTGC CACCAATGGG CTCGTCACAC TGGACAATAC CAACCTTCAA
    ATTCACAGGG AGCTGCTGCG CTGCGAGGCC GCGCTGGCCA GGCTGTACTG CCGCATGGCC
    CTGCTCAACA TCTTCGCCCC CAAGCTGCCT CACCTGTTTA CCCGCCTCTT CCACATCCCC
    GCCATCCGGG ACATTACCCT GGAGCACCTG CAGCTGCTGT CCAATCAGCT CCTCGCTCCT
    CCCCTCCCAG ATGGCACCAT CAGCTCCAGC TCCATCCTCC TGGCGCAGTC CCTGCAGCAC
    TGTGTCCATT CCCAGAACTG TTCGGCCACA GACCTCTTCT ACCAGGGCAG CTCCCAGACG
    GTGCGAGAGT GGCTCAACGT GGCCATCACG CGGACCCTGC ACCAGGGCGA GGAAAGCCTT
    CTGGAGCTGA CCAAACAGAT CTGCTCTTTC CTGCAGACAG CACCAGAACA GTTCCCCTCC
    GAAGAGTTTC CAATTTCCGA GTCCAAAGTC AACATGGACG TCAATTTCCC CGGGGCAGCT
    TTCGTCGTCG TATCTTGCAA AGAAAGTCAG TCTGGATTCC GCAAAGATTC CTCTCTGTAC
    AAGGCACCAT GGGCGCGTGT GCTGGTGTAC GGGCTAGGCC ACAAAGTGAA GCGAAATGGC
    CAGCTGAACC TCATTGAGGC CGTCTGTTAC CCGCGGGATG CGTCTCCGGC CAACACAGGG
    CTCACACCAC CTCCTACCAC CAACCAGTAC CCTTCTGTGA TCCTCTCCAC AGACAAAGTC
    CACATCAAAC TGGGGGTATC TCCCCCACCC GGAGCCGTGC TGGTGTTACA CTCCCTGCCT
    CTCGAGTTCC CCCTGGCGAT GGCCTTCACC GAGCAGCTAC TGTCCTGGAA ATCAGAGGAT
    GGCGAAGGTA GAGCTGAAGA TGAGCCTGAC ACCATCCCGA CGTCCGTCCT CCTGCAAGTG
    GTGGAGCTGC TTGGCAACTT CCTCTGGACC ACAGACATGG CGGCCTGCGT GAAGGAGCTG
    GTTTTCCACC TCCTGGCAGA GCTCCTGCGC ACGGTGCACA GCCTAGAGCA GAGGAAGCAC
    CCAGCCGGCC TGTCCTCATC CATCGCCCTC CAGCTGAACC CCTGCCTGGC CATGCTGATG
    GCCCTGCAGT CGGAGCTTCA CAAGCTGTAC GACGAGGAGA CGCAGAGCTG GGTCTCGGGC
    AGTGCATGCG GAGGCTCAGG AGCCACGGCG GCTGCTGACC AGGGCCGGTT CTCGACGTAT
    TTCCACGCAC TCATGGAGGG CTGCCTGGCG GTGGCCGAGG TGACCCTGCC CACCAACATG
    AGTGTCACCG CCAGCGGGGT GACGTCAGCA ACTGCCCCAA ACCTCAGCGA CTCGTCCTCC
    TCCTCATCGT CCTCCCCAGG ACAGACTCCA CAGAGCCCCA GCCTCCTGTC CAAGAGGAAG
    AAAGTCAAGA TGAAGCGGGA AAAGGCCTCC TCCTCTGGGA AGCGCCAGTC CTCACGCTCT
    GTGGACTCGG ACCCCGCCGT GCTGAGCATC GGGGGCAGCA AGCCCGAGGA CATGCTGTGG
    TTCCATCGTG CTCTGACGTT GCTCATCATC CTCCGCCACC TCACCAGGAA GGACCCACAG
    GGGCTAGGCG TGACAAGCGA CGCCATCGCC GACGCCTGCC AGGCCCTGGT GGGCCCCACC
    GCCCACAGCC GCTTGCTGGT GATCTCTGGG ATCCCCACCC ACCTGGACGA GGGCATCGTC
    AGGGGTGCCA TCCGCAAGGC CTGCAATGCC CATGGCGGGG TGTTCAAGGA TGAGATCTAC
    ATCCCCCTGC AGGACGAAGA CCCCAAGAAG CCAAAAGATA AGGCCGAGGG GGGTGATGGG
    AAAGCCGAGC CGGAAAAGAC CCTCGGCTTC CCCAGCACGG ACAGCTTGGA GGTCAGCACA
    TCCAGCAGCC TGACCCCCGC TATGAGCGTC AGCGCATCCG CGTCCACCAG CCAGGCTTCC
    ATCTGCAGCT CCCAGGGCAT CTCGCAGACA GTCAGTGACC TCTCTGCGGA TCCGTTGCCT
    GCAGGCCTGG AGCTGCCCAT CCCTGCAGGC TTGTTGGAGC CCCATGTGGT GTCCAGCCAG
    GAGAGCCTGG ACATTTCCCT GTGTAGCACC GGCAGCCTGG GCAGCCTGGG GAGCCTGGGC
    GAGCCACTGG ACAACGCAGA AACGGCCTCA GTGTCAGACA TGGGCTCCAT GTACACAGTC
    ACGTCCCTGG ACAACCAGCC CCTTGCAGCG CGCCCCATCA AAGGCTTCGC GGTCGTGGAG
    ATAAGGTCCC GAGCCAAAAT TGAGAAAATT CGAGCAAGTT TATTTAACAA CAATGACTTG
    ATAGGTTTGT CAAGCTTGGA TGGTGAGGAT GAATTGATGG AAATGTCAAC GGAAGAGATC
    CTCACGGTGT CTGTAGTGAA TCAGAGTCTA TTCGATACGC AGGGGAGCCC AGGGTTAGAA
    GATTATTTCA ATGATAAGTC AATTAAAGGT GAGAAGCTGG TGCCTGGGGC CAGAGAGGTG
    CTGACGGAGA TATTTAAGAG CTGTGCCCAC TCGGAGCAGA TGCTGAGCCT GACGCCAGCA
    AAGCCCATCA AAGTGTCGGA CATTTATCTT AGCAAAGAGC AGATCAACTC CCAGACACCG
    GGCAACCTCC TCCACCTTTT CTTCACCAAT GTCCGGCCCC CCAAAAAGGT GCTAGAGGAC
    CAGCTCACCC AGATCCTGAG GAAGTACGGT GTGCCGAAGC CCAAGTTTGA CAAGAGCAAG
    TACAGCAAGG CGGGGAAGGA GCAGCACCCC GGGAAGGTGG TGAGCACCAA GCGGCCCATC
    ACCAAGCCGC CCACCAAGGA CAAGGCAGTG CTCAACAGCG TCAGCAGGAC TGCCTTGAGT
    GAGAAGAAGC CCACCGTGAA GCCCAAGTCA CCTGAAAAGA GCAAGCCAGA TGAAAAGGAC
    CCTGAAAAGT CACCCACCAA GAAACAAGAC GTCCCAGAGG AGAAGTACCT GACCTTAGAA
    GGATTTCACA AGTTTGTGAT CGACCGAGCC AAGCAAGACA TCCGCAGCGT CTGGAGAGCG
    ATTTTATCCT GTGGCTACGA TCTTCATTTT GAGAGGTGTG CCTGCATCGA CGTCCGGCAT
    GCGCACAAGG CCTCCAGGAA GTGGACCCTG GAGATGGATG TGGCGCTCGT GCAGTACATC
    AACCGGCTGT GCCGCCACCT GGCCATCACG CCCGCGCGTC TGCATCCCCA CGAGGTGTAT
    CTGGACCCTG CAGACGCTGC TGACCCCAGA GTGGCCTGTC TCTTGAACGT GCCCATCGAG
    AGCCTGCGCC TGCGCTTCGC TCTGCTGCAG TCCCTCAACA CCACGCTCGA GACCTTCTTC
    CTGCCGCTGG TTGAGCTGCG CCAGACTCCC ATGTACACGC ACAGCATCGC CGCCCTGCTC
    AAGGAGGCCA AAGGGCTGAT CTTTTATGAC ACAAAGGTGA CCGTCATGAA TCGGGTGCTA
    AATGCCACGG TGCAGAGGAC GGCTGACCAT GCAGCACCAG AAATCACTCT GGACCCGCTG
    GAGATCGTGG GAGGGGAGAT CAGAGCCTCT GAGAACTCCT ACTTCTGCCA GGCAGCCAGG
    CAGCTGGCCT CAGTGCCCTC CTCCCAGCTT TGCGTCAAAC TGGCGAGTGG CGGCGACCCT
    ACCTATGCCT TCAACATCCG CTTCACGGGA GAGGAGGTCC ATGGCACCAG TGGCTCCTTC
    CGCCACTTCC TGTGGCAGGT GTGCAGAGAG CTGCAGAGCT CCTCGCTCTC CCTCCTCCTG
    CTGTGCCCCA GCTCCGCCGT CAACAAGAAC AAGGGCAAGT ACATCCTGAC CCCCAGCCCC
    ATCACCTATG GGGAGGAGCA GCTGCTGCAC TTCCTGGGCC AGTTGCTAGG GATCGCCATC
    CGAGCAGATG TCCCCCTCCC CCTGGACCTC CTGCCCTCCT TCTGGAAGAC ACTGGTGGGC
    GAGCCCCTGG ACCCTGACCA AGACCTGCAG GAAGCAGACG TACTCACCTA CAATTACGTC
    AAGAAATTTG AGAGTATCAA CGACGAGACG GAGTTGGAGG CCCTGTGTGC TGAGATCGCC
    TCCCAGCACC TGGCCACTGA GAGCCCCGAG GGGCCCAACA AGCCCTGCTG CCGGTTCACC
    TACCTGACCA TGACCGGCGA GGAGGTGGAG CTGTGCAGCC GTGGCCGGCA CATCCCCGTG
    GCGTGGGAGA ACAAGGACAT CTATGCGGCC GCCATCCGGA GCCTGCGGCT GCGTGAGCTA
    CAGAACGTGG AGTGTGTGAC GGCCGTGCGC GCGGGCCTTG GGGCCATCAT CCCCCTGCAG
    CTGCTCACCA TGCTCAGCCC ACTGGAGATG GAGCTGCGCA CCTGCGGCCT CCCGTACATC
    AACCTCGAGT TCCTGAAGGC CCACACCATG TACCAGGTGG GGCTGATGGA GACCGACCAG
    CACATCGAGT TCTTCTGGGG GGCACTGGAG ATGTTTGCCC AGGAGGAGCT GTGCAAGTTC
    ATCAAGTTTG CCTGCAACCA GGAGCGCATC CCGTTCACCT GCCCCTGCAA AGACGGGGGT
    CCTGACACGG CCCATGTGCC CCCATACCCC ATGAAGATTG CCCCCCCAGA TGGCACAGCA
    GGTTCCCCAG ATTCTCGCTA CATCCGAGTG GAGACCTGTA TGTTCATGAT CAAGCTTCCC
    CAGTACTCCT CGCTGGAAAT CATGCTGGAG AAACTACGTT GTGCCATTCA CTACCGCGAA
    GACCCTCTGA GTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812360.2
    CDS284..13561
    Translation

    Target ORF information:

    RefSeq Version XM_010814058.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814058.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATCTAACAGT ATATTTTCTG TTTTGAAGTT GGTGATATAC
    GCGGGGCCTA ACACAAACAG TAGGAAGGTT GCTGAGTATG GAGGCAATAC ACTGGGATAT
    GGCAGCCGTA GTGTCTTAGG AACTGGTTGG CCGAAAGACT TGGTGAAGGT GGAAGGAGAT
    ACAGTCACCT TCTCCTTTGA AATGAGAAGT GGCCGTGAAC ACAACACTCC CGATAAGGCT
    ATGTGGGGCT TTGCTTGCAC AGTTCGCGCT CAGGAGTCTT CGGAGGATGT GTCAGGAGGC
    TTACCCTTTC TGGTAGACCT GGCTTTAGGT CTATCTGTGT TAGCTTGTTC CATGTTAAGA
    ATCCTATACA ACGGACCAGA AATTACCAAA GAAGAAGAAA CCTGTCAGGA GCTGTTGCGG
    TCCAAACTTT TACAAAGGTG CCAGTGGCAG GTAGAGGCCA ACGGTGTGAT CTCCCCTGCC
    CTTACTCCAA GCCCATCTCC ACTGCCTCTG ACCATCGAGG AAGACAGAGA GTTCACTTAC
    CCGTCTGATG TCCTCGTGCC TCCTGTTGGA AACTACTTTG ACCTGCCCCG GATCAGGCTG
    CCTCCAGGAA TCATGATAAA GCTCAGGGAA ATTTCTGGGC GTGCTAGACC ACAGTTTAGA
    CCAAGTATAA AAGAAGTAAT TCAGCCAGAT GTGATGGAGG AAATGGTGGT ATCATGTGTT
    ATTAAGCACT TGAACTTAGT TGATGCACTG CAGTCCCTAA TAAATTTCCA GTATCAAGAA
    GAACATGCTG AGGAATATGA TTTACTGTGT AAAATCATGG GAGAGACATT TAAGAAACTC
    AATGCCATGG AGAGACAGCT GCAGAGTGTT GCAGAATTGG AACAGAAATG GCAGAGTGAA
    GTTGATGATG CCATGCAGGG GAAACTGGAG AACAACATGC CTTTCTTCTA TGATTACCAT
    TTCAATGAGA ACAAAATGAA AGAACTAGAA CTTTTGTGTT CAATGAAGGA AGTCTCCTTT
    GATGGAAATG ATCTTGAAAA CATGGTGCTA TCTCTGAGGG AGAAGTTCCT ACAAGAAGTG
    AATTCTCTTA TTCAGAAACC CTCGCACCCA CTGGCTAAAA CGAAGACGTT AGTGAAGAGT
    CTAATGAACC GAGCTGAGCT GTTGCTGCAC GTCACCATTG CAGCCCAGTC TGGCCTCACG
    AGAAGCATCT CAGGGACCCC CGCGGAAACA CCGGCTTGTA AATCAGCCTC TGAAACAAAG
    GTGATCTCTC ATGCTGTGCG TCAGCCTGTT TTCCTTCGCA GCATGTCGGC TCCCTCTGAC
    CTGGAAATGA TCGGTAATGA AGATTTGGAA TTTACTAGAG CAAATCAGAG GCGGCGCCAC
    GTGACCAGCC ACCGCAGCAG CTCCTTCACA CTCCTGCAGT CATTGGCCAT AGAGGACAGC
    AGGGACAAGC CCACCTACAG CGTCCTGCTG GGGCAGCTGT TTGCTTTCAT CGGCACCAAC
    CCTGACCAAG CTGTGTCCAG CAGCAGCTTT CTTCTGGCCG CACAGACGAG ATGGCGGCGG
    GGAAACACCC GCAAGCAGGC GTTGGTGCAC ATGCGGGAGC TGCTGACGGC TGCCGTGCGA
    GTCGGGGGAG TGACACACCT TGTGGGTCCA GTGACAATGG TCCTGCAGGG AGGACCCAGA
    ATTGAAGAAC TGACCTGTGG TGGGATGGTC GAGCAGGTCC AGGAAGCCTT CGGTGAGACC
    ATGACCTCTG TGGTGTCTTT GTGCGCACGT TACCCTATCG CATGTGCAAA TAGCATCGGA
    CTCTTGTGCA CTATACCTTA TACCAGGAGT GAAGAGAAGT GCCTGGTGCG GAGTGGCCTT
    GTCCAGCTCA TGGATCGACT ATGCAGCTTG AGCAGTCAGA CTGAGTCCAG CTCCAGTGAG
    AAACAAACCA AGAAGCAGAA GGTGGCCACC ATGGCCTGGG CAGCCTTCCA GGTGCTTGCC
    AACCGCTGCG TTGAATGGGA AAAAGAAGAA GGCGGCTCCA CAGAGGCTGT GCACTCAGGT
    CTGGCCCGTC AGGTGTCCAG CCTTCTCACG AATCACCTTG CCCGAGCGAC CGAGTGCTGT
    GGCAACCAGG CTGCCGGGAA CGATGCCCTC CAGGACGTCC TGAGTCTCCT CAATGATCTC
    TCGAGGAGTC ATATAGGTAA AGCCATCCTG AGCCAGCCAG CTTGTGTGTC CAAACTCCTC
    TCCCTGCTAC TTGACCAACG CCCATCCCCA AAGCTGGTTC TTATCATTCT TCAGCTGTGC
    CGGGCGGCAC TCCCGCTGAT GAGCGTAGAA GACTGTGGGA ACGTGGAGCT CCCACCCTGG
    AGCTACTCCG TCCCCTCCCT AAACAGCGAG CAGGAGGATC CCAGCGACCC AGCGTCCAAG
    ATCGCCTCGC TGCTCTTGGC AAAGCTGGCA GACTACGTGG TCCCAGGATG TCAGACAGTC
    CTTTCTCCAA CCGCTTCCGA ACCTGACACC ACATTGACAA AAACCAGCCC CAAGAATTCC
    TTAAAAGGCG ATAAAGATCC TGGAGAAGAG AGTGAAGCAG TGGATGGCAA GCTCTCTATC
    TTCATCCACA AGCGGGAAGA CCAGTCGTCC CATGAAGTTC TGCAGCCATT ACTAAGTAGC
    TCAGAAGGAC GGCCCTTCCG ACTTGGTACT GGTGCCAACA TGGAGAAAGT TGTGAAAATG
    GACCGAGACA TGACGAAGGG TGGCTGCTGT GAGGTGATTA CGGAAGAGGC GGCAGCTGCT
    CTGCGAAAAG CGACCAAGTG GGCACAGTCA GGCCTCATTG TCAGTGTTGG GCCACCCGTG
    GAGTCCCTCA ACCCAGAGGC CGTGAGCGGG CTGTCCACAG GGGACAAGAA GAAGACTGCC
    CAAACCTCCA TTTGCCGAGA GAGAAACTCT GAACTCGCCA GGACCGACCC CGTCCGACCT
    TTCATCAGTG GGCACGTGGC AAACAGCATG GCTGCTGAGG TGATCGCCTT GCTTCATAGC
    TTGCTGATGG CACCTGAGTC AAATGCTGCT CAGATATGGA CCACGACAGC AGAGAAGGTT
    CTGTCTCGTG CACTGATGTA CATTCCACAG TTGGGGAAAT ATGCAGAAAG CATTCTGGAA
    AATGGCAGCA GCAGTGGCAG AAAGCTTGCC AAACTTCAGA GAATTGCCCG CCAGGCTGTG
    GCTGCACTCT GTGCACTTGG AGGCTTCAAA GAGACAATCA AGATCGGGTC TGAGGTCCAG
    GTTTTAGGTA GAGGAATATC AGGAAGCATC GGAGTGGTGG CTTCTATCAA CGAACAGGAA
    GGTATAGCTA CGGTCAGATT CCCACCCATA GACTGTAGAA AGACTTCGCA AGCATCAGAC
    ACACTGACTA TTCCATTGTC TAGACTTTGT GTTCCAAGAT CAGAGGCATT GCCTCTTCAT
    AAACTGTCCA TTACTGAGAA GGTAGTACAG GCAGTCCAGT CCATGTTGCT TCCTCAGGAG
    GGAAGTCTCT CTATTCATAC CTCACTTCCT GCAGCAGGAG ATGGGTCAGC TCCTGTGATG
    GCAGTCGTTC GGCTCCTGGC TGAAATAAGA ACAAGAGCAT GCCTGGTCAT GGCCCAGCTT
    TTAGAAGACA GCTTATTTTG TGAAGAATTC ATACAGCAGT GTCCAGCAGC TGTTGAAGTT
    CTTAACCTAG TAGCCCAGGA ATGCAGTGCT GGAGAGCGGC TGGCAGTTGT GGAAGTGCAG
    TGTGAACGGC TGAGGATGCT CTACCGGGAC TGTGCTCGGC CCCCGCCGCC TCCGCTGCAG
    GCTGACCGGA GACAGCCCAA AGAGATTACG TGGAGCCCCT CACGAGTGTT TCCACCTGTC
    CGAGCCTGCA TGTTCTCCTC GCACCTCACT TCTGTCACCT TCCTAGCAGA CCCCAGTGCA
    GGGGGCGGTC TGCCCAGGGG CACATTTATC TACGCCACCT CACCACTGCC CGTTCAGGCC
    CCGTCTTTCT ACTGGGAAAT TGAAATTGTT TCCTATGGAG ATACAGATGA TGATACTGGA
    CCAATAGTTT CCTTTGGCTT TGCTACAGAA GCAGAGAAAA GAGATGGGGC ATGGACTAAT
    CCAGTGGGCA CCTGTCTTTT CCATAACAAT GGCCGAGCTG TGCATTACAA TGGCTCCAGT
    TTATTACAGT GGAAGAGCGT TCGATTAGAC GTAACTCTCT CTCCTGGTGA CGTGGCAGGG
    ATTGGCTGGG AGAGAACCGA GGGGACGCCT CCTCCACCCG GGCAGCCTGC CAAGGGCCGT
    GTGTACTTCA CATACTGTGG GCAGCGCCTC TCACCGTATC TCGAAGATGT CTCGGGTGGG
    ATGTGGCCGG TGGTTCATAT TCAGAAAAAG AACACCAAGA CTCGCGCTAA TTTTGGCTCC
    CGCCCATTTG CCTACGCTGA AGGGCAAGCC CACCGCAATG CTGCCGACCT GTGCACTGAC
    CTGGCCGAGG AAATTAGCGC TAACTTCGAG GCCCTGCCCT TTGCCATGGC CTCTGACAGT
    GATAACGATG CTGGCACCAG TATTGCGTCA GACCCGGGCA CTCATGGGCC ACCATGCCGA
    ATTGCTGCCG TAGCCACTGC TCAGCAACAA TATGATAGTG ATACTTCCTG TCATTATAAA
    GTGGAGCTCA GCTATGAGAA TTTCATCACC TCTGGGCCAG ACCCTCACCC ACCCCCCATT
    GCAGATGACG AAAGTGATGA TGATGATGAC GATGACATCC CACAGGAGGA TCACTATGCC
    CTGCTGGTGA AAGCCTGGGA GACCAAGGTT TTTCCTACCA TCCGAAGACG GTTCCGCAAC
    GAAGCAGAGA GGAAATCAGG ACTGGACCAG ATAAAGGGTG CTTTACAACT AGGCATGGTG
    GATATTGCCC GGCAGACAGT TGAATTCCTC TACGAGGAGA ATGGTGGCAT CCCAAGAGAC
    CTTTATCTTC CCACCATTGA AGACATTAAA GATGAAGCGA ACAAGTTCAC AATTGATAAA
    GTTCGAAAAG GTCTCACGGT AGTAACACGC TCTCCAGACA GCAATAACGT AGCCAGCAGC
    ACTGTTGGAA CTGCCCTGCC AAAATTTGCT ATCCGAGGGA TGCTGAAAAC CTTTGGGCTT
    CATGGAGTTG TCTTAGATGT TGATTCAGTT AATGAACTAG TTCAGGTAGA AACGTACCTC
    CGAAGTGAAG GTGTGCTGGT CCGATACTGG TACCCTATTG ATATGTTGGA AAGGCCCCCC
    GCAGGCTACA GAAGGACTGC CACCAATGGG CTCGTCACAC TGGACAATAC CAACCTTCAA
    ATTCACAGGG AGCTGCTGCG CTGCGAGGCC GCGCTGGCCA GGCTGTACTG CCGCATGGCC
    CTGCTCAACA TCTTCGCCCC CAAGCTGCCT CACCTGTTTA CCCGCCTCTT CCACATCCCC
    GCCATCCGGG ACATTACCCT GGAGCACCTG CAGCTGCTGT CCAATCAGCT CCTCGCTCCT
    CCCCTCCCAG ATGGCACCAT CAGCTCCAGC TCCATCCTCC TGGCGCAGTC CCTGCAGCAC
    TGTGTCCATT CCCAGAACTG TTCGGCCACA GACCTCTTCT ACCAGGGCAG CTCCCAGACG
    GTGCGAGAGT GGCTCAACGT GGCCATCACG CGGACCCTGC ACCAGGGCGA GGAAAGCCTT
    CTGGAGCTGA CCAAACAGAT CTGCTCTTTC CTGCAGACAG CACCAGAACA GTTCCCCTCC
    GAAGAGTTTC CAATTTCCGA GTCCAAAGTC AACATGGACG TCAATTTCCC CGGGGCAGCT
    TTCGTCGTCG TATCTTGCAA AGAAAGTCAG TCTGGATTCC GCAAAGATTC CTCTCTGTAC
    AAGGCACCAT GGGCGCGTGT GCTGGTGTAC GGGCTAGGCC ACAAAGTGAA GCGAAATGGC
    CAGCTGAACC TCATTGAGGC CGTCTGTTAC CCGCGGGATG CGTCTCCGGC CAACACAGGG
    CTCACACCAC CTCCTACCAC CAACCAGTAC CCTTCTGTGA TCCTCTCCAC AGACAAAGTC
    CACATCAAAC TGGGGGTATC TCCCCCACCC GGAGCCGTGC TGGTGTTACA CTCCCTGCCT
    CTCGAGTTCC CCCTGGCGAT GGCCTTCACC GAGCAGCTAC TGTCCTGGAA ATCAGAGGAT
    GGCGAAGGTA GAGCTGAAGA TGAGCCTGAC ACCATCCCGA CGTCCGTCCT CCTGCAAGTG
    GTGGAGCTGC TTGGCAACTT CCTCTGGACC ACAGACATGG CGGCCTGCGT GAAGGAGCTG
    GTTTTCCACC TCCTGGCAGA GCTCCTGCGC ACGGTGCACA GCCTAGAGCA GAGGAAGCAC
    CCAGCCGGCC TGTCCTCATC CATCGCCCTC CAGCTGAACC CCTGCCTGGC CATGCTGATG
    GCCCTGCAGT CGGAGCTTCA CAAGCTGTAC GACGAGGAGA CGCAGAGCTG GGTCTCGGGC
    AGTGCATGCG GAGGCTCAGG AGCCACGGCG GCTGCTGACC AGGGCCGGTT CTCGACGTAT
    TTCCACGCAC TCATGGAGGG CTGCCTGGCG GTGGCCGAGG TGACCCTGCC CACCAACATG
    AGTGTCACCG CCAGCGGGGT GACGTCAGCA ACTGCCCCAA ACCTCAGCGA CTCGTCCTCC
    TCCTCATCGT CCTCCCCAGG ACAGACTCCA CAGAGCCCCA GCCTCCTGTC CAAGAGGAAG
    AAAGTCAAGA TGAAGCGGGA AAAGGCCTCC TCCTCTGGGA AGCGCCAGTC CTCACGCTCT
    GTGGACTCGG ACCCCGCCGT GCTGAGCATC GGGGGCAGCA AGCCCGAGGA CATGCTGTGG
    TTCCATCGTG CTCTGACGTT GCTCATCATC CTCCGCCACC TCACCAGGAA GGACCCACAG
    GGGCTAGGCG TGACAAGCGA CGCCATCGCC GACGCCTGCC AGGCCCTGGT GGGCCCCACC
    GCCCACAGCC GCTTGCTGGT GATCTCTGGG ATCCCCACCC ACCTGGACGA GGGCATCGTC
    AGGGGTGCCA TCCGCAAGGC CTGCAATGCC CATGGCGGGG TGTTCAAGGA TGAGATCTAC
    ATCCCCCTGC AGGACGAAGA CCCCAAGAAG CCAAAAGATA AGGCCGAGGG GGGTGATGGG
    AAAGCCGAGC CGGAAAAGAC CCTCGGCTTC CCCAGCACGG ACAGCTTGGA GGTCAGCACA
    TCCAGCAGCC TGACCCCCGC TATGAGCGTC AGCGCATCCG CGTCCACCAG CCAGGCTTCC
    ATCTGCAGCT CCCAGGGCAT CTCGCAGACA GTCAGTGACC TCTCTGCGGA TCCGTTGCCT
    GCAGGCCTGG AGCTGCCCAT CCCTGCAGGC TTGTTGGAGC CCCATGTGGT GTCCAGCCAG
    GAGAGCCTGG ACATTTCCCT GTGTAGCACC GGCAGCCTGG GCAGCCTGGG GAGCCTGGGC
    GAGCCACTGG ACAACGCAGA AACGGCCTCA GTGTCAGACA TGGGCTCCAT GTACACAGTC
    ACGTCCCTGG ACAACCAGCC CCTTGCAGCG CGCCCCATCA AAGGCTTCGC GGTCGTGGAG
    ATAAGGTCCC GAGCCAAAAT TGAGAAAATT CGAGCAAGTT TATTTAACAA CAATGACTTG
    ATAGGTTTGT CAAGCTTGGA TGGTGAGGAT GAATTGATGG AAATGTCAAC GGAAGAGATC
    CTCACGGTGT CTGTAGTGAA TCAGAGTCTA TTCGATACGC AGGGGAGCCC AGGGTTAGAA
    GATTATTTCA ATGATAAGTC AATTAAAGGT GAGAAGCTGG TGCCTGGGGC CAGAGAGGTG
    CTGACGGAGA TATTTAAGAG CTGTGCCCAC TCGGAGCAGA TGCTGAGCCT GACGCCAGCA
    AAGCCCATCA AAGTGTCGGA CATTTATCTT AGCAAAGAGC AGATCAACTC CCAGACACCG
    GGCAACCTCC TCCACCTTTT CTTCACCAAT GTCCGGCCCC CCAAAAAGGT GCTAGAGGAC
    CAGCTCACCC AGATCCTGAG GAAGTACGGT GTGCCGAAGC CCAAGTTTGA CAAGAGCAAG
    TACAGCAAGG CGGGGAAGGA GCAGCACCCC GGGAAGGTGG TGAGCACCAA GCGGCCCATC
    ACCAAGCCGC CCACCAAGGA CAAGGCAGTG CTCAACAGCG TCAGCAGGAC TGCCTTGAGT
    GAGAAGAAGC CCACCGTGAA GCCCAAGTCA CCTGAAAAGA GCAAGCCAGA TGAAAAGGAC
    CCTGAAAAGT CACCCACCAA GAAACAAGAC GTCCCAGAGG AGAAGTACCT GACCTTAGAA
    GGATTTCACA AGTTTGTGAT CGACCGAGCC AAGCAAGACA TCCGCAGCGT CTGGAGAGCG
    ATTTTATCCT GTGGCTACGA TCTTCATTTT GAGAGGTGTG CCTGCATCGA CGTCCGGCAT
    GCGCACAAGG CCTCCAGGAA GTGGACCCTG GAGATGGATG TGGCGCTCGT GCAGTACATC
    AACCGGCTGT GCCGCCACCT GGCCATCACG CCCGCGCGTC TGCATCCCCA CGAGGTGTAT
    CTGGACCCTG CAGACGCTGC TGACCCCAGA GTGGCCTGTC TCTTGAACGT GCCCATCGAG
    AGCCTGCGCC TGCGCTTCGC TCTGCTGCAG TCCCTCAACA CCACGCTCGA GACCTTCTTC
    CTGCCGCTGG TTGAGCTGCG CCAGACTCCC ATGTACACGC ACAGCATCGC CGCCCTGCTC
    AAGGAGGCCA AAGGGCTGAT CTTTTATGAC ACAAAGGTGA CCGTCATGAA TCGGGTGCTA
    AATGCCACGG TGCAGAGGAC GGCTGACCAT GCAGCACCAG AAATCACTCT GGACCCGCTG
    GAGATCGTGG GAGGGGAGAT CAGAGCCTCT GAGAACTCCT ACTTCTGCCA GGCAGCCAGG
    CAGCTGGCCT CAGTGCCCTC CTCCCAGCTT TGCGTCAAAC TGGCGAGTGG CGGCGACCCT
    ACCTATGCCT TCAACATCCG CTTCACGGGA GAGGAGGTCC ATGGCACCAG TGGCTCCTTC
    CGCCACTTCC TGTGGCAGGT GTGCAGAGAG CTGCAGAGCT CCTCGCTCTC CCTCCTCCTG
    CTGTGCCCCA GCTCCGCCGT CAACAAGAAC AAGGGCAAGT ACATCCTGAC CCCCAGCCCC
    ATCACCTATG GGGAGGAGCA GCTGCTGCAC TTCCTGGGCC AGTTGCTAGG GATCGCCATC
    CGAGCAGATG TCCCCCTCCC CCTGGACCTC CTGCCCTCCT TCTGGAAGAC ACTGGTGGGC
    GAGCCCCTGG ACCCTGACCA AGACCTGCAG GAAGCAGACG TACTCACCTA CAATTACGTC
    AAGAAATTTG AGAGTATCAA CGACGAGACG GAGTTGGAGG CCCTGTGTGC TGAGATCGCC
    TCCCAGCACC TGGCCACTGA GAGCCCCGAG GGGCCCAACA AGCCCTGCTG CCGGTTCACC
    TACCTGACCA TGACCGGCGA GGAGGTGGAG CTGTGCAGCC GTGGCCGGCA CATCCCCGTG
    GCGTGGGAGA ACAAGGACAT CTATGCGGCC GCCATCCGGA GCCTGCGGCT GCGTGAGCTA
    CAGAACGTGG AGTGTGTGAC GGCCGTGCGC GCGGGCCTTG GGGCCATCAT CCCCCTGCAG
    CTGCTCACCA TGCTCAGCCC ACTGGAGATG GAGCTGCGCA CCTGCGGCCT CCCGTACATC
    AACCTCGAGT TCCTGAAGGC CCACACCATG TACCAGGTGG GGCTGATGGA GACCGACCAG
    CACATCGAGT TCTTCTGGGG GGCACTGGAG ATGTTTGCCC AGGAGGAGCT GTGCAAGTTC
    ATCAAGTTTG CCTGCAACCA GGAGCGCATC CCGTTCACCT GCCCCTGCAA AGACGGGGGT
    CCTGACACGG CCCATGTGCC CCCATACCCC ATGAAGATTG CCCCCCCAGA TGGCACAGCA
    GGTTCCCCAG ATTCTCGCTA CATCCGAGTG GAGACCTGTA TGTTCATGAT CAAGCTTCCC
    CAGTACTCCT CGCTGGAAAT CATGCTGGAG AAACTACGTT GTGCCATTCA CTACCGCGAA
    GACCCTCTGA GTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224640
    Clone ID Related Accession (Same CDS sequence) XM_010814059.3
    Accession Version XM_010814059.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13257bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814059.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    541
    601
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    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
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    2641
    2701
    2761
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    3001
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    5281
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    5401
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    5521
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    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
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    6301
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    6421
    6481
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    7141
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    9721
    9781
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    9901
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    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
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    10921
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    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA
    ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAGGCTA TGTGGGGCTT TGCTTGCACA
    GTTCGCGCTC AGGAGTCTTC GGAGGATGTG TCAGGAGGCT TACCCTTTCT GGTAGACCTG
    GCTTTAGGTC TATCTGTGTT AGCTTGTTCC ATGTTAAGAA TCCTATACAA CGGACCAGAA
    ATTACCAAAG AAGAAGAAAC CTGTCAGGAG CTGTTGCGGT CCAAACTTTT ACAAAGGTGC
    CAGTGGCAGG TAGAGGCCAA CGGTGTGATC TCCCCTGCCC TTACTCCAAG CCCATCTCCA
    CTGCCTCTGA CCATCGAGGA AGACAGAGAG TTCACTTACC CGTCTGATGT CCTCGTGCCT
    CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG
    CTCAGGGAAA TTTCTGGGCG TGCTAGACCA CAGTTTAGAC CAAGTATAAA AGAAGTAATT
    CAGCCAGATG TGATGGAGGA AATGGTGGTA TCATGTGTTA TTAAGCACTT GAACTTAGTT
    GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT
    TTACTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG
    CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAGAGTGAAG TTGATGATGC CATGCAGGGG
    AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TCAATGAGAA CAAAATGAAA
    GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC
    ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTTAT TCAGAAACCC
    TCGCACCCAC TGGCTAAAAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG
    TTGCTGCACG TCACCATTGC AGCCCAGTCT GGCCTCACGA GAAGCATCTC AGGGACCCCC
    GCGGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATCTCTCA TGCTGTGCGT
    CAGCCTGTTT TCCTTCGCAG CATGTCGGCT CCCTCTGACC TGGAAATGAT CGGTAATGAA
    GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC
    TCCTTCACAC TCCTGCAGTC ATTGGCCATA GAGGACAGCA GGGACAAGCC CACCTACAGC
    GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC
    AGCAGCTTTC TTCTGGCCGC ACAGACGAGA TGGCGGCGGG GAAACACCCG CAAGCAGGCG
    TTGGTGCACA TGCGGGAGCT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACACCTT
    GTGGGTCCAG TGACAATGGT CCTGCAGGGA GGACCCAGAA TTGAAGAACT GACCTGTGGT
    GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT GGTGTCTTTG
    TGCGCACGTT ACCCTATCGC ATGTGCAAAT AGCATCGGAC TCTTGTGCAC TATACCTTAT
    ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG AGTGGCCTTG TCCAGCTCAT GGATCGACTA
    TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG
    GTGGCCACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA
    AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC
    CTTCTCACGA ATCACCTTGC CCGAGCGACC GAGTGCTGTG GCAACCAGGC TGCCGGGAAC
    GATGCCCTCC AGGACGTCCT GAGTCTCCTC AATGATCTCT CGAGGAGTCA TATAGGTAAA
    GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTACT TGACCAACGC
    CCATCCCCAA AGCTGGTTCT TATCATTCTT CAGCTGTGCC GGGCGGCACT CCCGCTGATG
    AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC CCACCCTGGA GCTACTCCGT CCCCTCCCTA
    AACAGCGAGC AGGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA
    AAGCTGGCAG ACTACGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCCGAA
    CCTGACACCA CATTGACAAA AACCAGCCCC AAGAATTCCT TAAAAGGCGA TAAAGATCCT
    GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TCATCCACAA GCGGGAAGAC
    CAGTCGTCCC ATGAAGTTCT GCAGCCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA
    CTTGGTACTG GTGCCAACAT GGAGAAAGTT GTGAAAATGG ACCGAGACAT GACGAAGGGT
    GGCTGCTGTG AGGTGATTAC GGAAGAGGCG GCAGCTGCTC TGCGAAAAGC GACCAAGTGG
    GCACAGTCAG GCCTCATTGT CAGTGTTGGG CCACCCGTGG AGTCCCTCAA CCCAGAGGCC
    GTGAGCGGGC TGTCCACAGG GGACAAGAAG AAGACTGCCC AAACCTCCAT TTGCCGAGAG
    AGAAACTCTG AACTCGCCAG GACCGACCCC GTCCGACCTT TCATCAGTGG GCACGTGGCA
    AACAGCATGG CTGCTGAGGT GATCGCCTTG CTTCATAGCT TGCTGATGGC ACCTGAGTCA
    AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC ACTGATGTAC
    ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA
    AAGCTTGCCA AACTTCAGAG AATTGCCCGC CAGGCTGTGG CTGCACTCTG TGCACTTGGA
    GGCTTCAAAG AGACAATCAA GATCGGGTCT GAGGTCCAGG TTTTAGGTAG AGGAATATCA
    GGAAGCATCG GAGTGGTGGC TTCTATCAAC GAACAGGAAG GTATAGCTAC GGTCAGATTC
    CCACCCATAG ACTGTAGAAA GACTTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT
    AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG
    GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC
    TCACTTCCTG CAGCAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT
    GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT
    GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAAGTTC TTAACCTAGT AGCCCAGGAA
    TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC
    TACCGGGACT GTGCTCGGCC CCCGCCGCCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA
    GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCCTCG
    CACCTCACTT CTGTCACCTT CCTAGCAGAC CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC
    ACATTTATCT ACGCCACCTC ACCACTGCCC GTTCAGGCCC CGTCTTTCTA CTGGGAAATT
    GAAATTGTTT CCTATGGAGA TACAGATGAT GATACTGGAC CAATAGTTTC CTTTGGCTTT
    GCTACAGAAG CAGAGAAAAG AGATGGGGCA TGGACTAATC CAGTGGGCAC CTGTCTTTTC
    CATAACAATG GCCGAGCTGT GCATTACAAT GGCTCCAGTT TATTACAGTG GAAGAGCGTT
    CGATTAGACG TAACTCTCTC TCCTGGTGAC GTGGCAGGGA TTGGCTGGGA GAGAACCGAG
    GGGACGCCTC CTCCACCCGG GCAGCCTGCC AAGGGCCGTG TGTACTTCAC ATACTGTGGG
    CAGCGCCTCT CACCGTATCT CGAAGATGTC TCGGGTGGGA TGTGGCCGGT GGTTCATATT
    CAGAAAAAGA ACACCAAGAC TCGCGCTAAT TTTGGCTCCC GCCCATTTGC CTACGCTGAA
    GGGCAAGCCC ACCGCAATGC TGCCGACCTG TGCACTGACC TGGCCGAGGA AATTAGCGCT
    AACTTCGAGG CCCTGCCCTT TGCCATGGCC TCTGACAGTG ATAACGATGC TGGCACCAGT
    ATTGCGTCAG ACCCGGGCAC TCATGGGCCA CCATGCCGAA TTGCTGCCGT AGCCACTGCT
    CAGCAACAAT ATGATAGTGA TACTTCCTGT CATTATAAAG TGGAGCTCAG CTATGAGAAT
    TTCATCACCT CTGGGCCAGA CCCTCACCCA CCCCCCATTG CAGATGACGA AAGTGATGAT
    GATGATGACG ATGACATCCC ACAGGAGGAT CACTATGCCC TGCTGGTGAA AGCCTGGGAG
    ACCAAGGTTT TTCCTACCAT CCGAAGACGG TTCCGCAACG AAGCAGAGAG GAAATCAGGA
    CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT
    GAATTCCTCT ACGAGGAGAA TGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA
    GACATTAAAG ATGAAGCGAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA
    GTAACACGCT CTCCAGACAG CAATAACGTA GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA
    AAATTTGCTA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT
    GATTCAGTTA ATGAACTAGT TCAGGTAGAA ACGTACCTCC GAAGTGAAGG TGTGCTGGTC
    CGATACTGGT ACCCTATTGA TATGTTGGAA AGGCCCCCCG CAGGCTACAG AAGGACTGCC
    ACCAATGGGC TCGTCACACT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC
    TGCGAGGCCG CGCTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC
    AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCCG CCATCCGGGA CATTACCCTG
    GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC
    AGCTCCAGCT CCATCCTCCT GGCGCAGTCC CTGCAGCACT GTGTCCATTC CCAGAACTGT
    TCGGCCACAG ACCTCTTCTA CCAGGGCAGC TCCCAGACGG TGCGAGAGTG GCTCAACGTG
    GCCATCACGC GGACCCTGCA CCAGGGCGAG GAAAGCCTTC TGGAGCTGAC CAAACAGATC
    TGCTCTTTCC TGCAGACAGC ACCAGAACAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG
    TCCAAAGTCA ACATGGACGT CAATTTCCCC GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA
    GAAAGTCAGT CTGGATTCCG CAAAGATTCC TCTCTGTACA AGGCACCATG GGCGCGTGTG
    CTGGTGTACG GGCTAGGCCA CAAAGTGAAG CGAAATGGCC AGCTGAACCT CATTGAGGCC
    GTCTGTTACC CGCGGGATGC GTCTCCGGCC AACACAGGGC TCACACCACC TCCTACCACC
    AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTATCT
    CCCCCACCCG GAGCCGTGCT GGTGTTACAC TCCCTGCCTC TCGAGTTCCC CCTGGCGATG
    GCCTTCACCG AGCAGCTACT GTCCTGGAAA TCAGAGGATG GCGAAGGTAG AGCTGAAGAT
    GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAGCTGCT TGGCAACTTC
    CTCTGGACCA CAGACATGGC GGCCTGCGTG AAGGAGCTGG TTTTCCACCT CCTGGCAGAG
    CTCCTGCGCA CGGTGCACAG CCTAGAGCAG AGGAAGCACC CAGCCGGCCT GTCCTCATCC
    ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC GGAGCTTCAC
    AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GTGCATGCGG AGGCTCAGGA
    GCCACGGCGG CTGCTGACCA GGGCCGGTTC TCGACGTATT TCCACGCACT CATGGAGGGC
    TGCCTGGCGG TGGCCGAGGT GACCCTGCCC ACCAACATGA GTGTCACCGC CAGCGGGGTG
    ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC TCGTCCTCCT CCTCATCGTC CTCCCCAGGA
    CAGACTCCAC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA
    AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCACGCTCTG TGGACTCGGA CCCCGCCGTG
    CTGAGCATCG GGGGCAGCAA GCCCGAGGAC ATGCTGTGGT TCCATCGTGC TCTGACGTTG
    CTCATCATCC TCCGCCACCT CACCAGGAAG GACCCACAGG GGCTAGGCGT GACAAGCGAC
    GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGCCCCACCG CCCACAGCCG CTTGCTGGTG
    ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GGGGTGCCAT CCGCAAGGCC
    TGCAATGCCC ATGGCGGGGT GTTCAAGGAT GAGATCTACA TCCCCCTGCA GGACGAAGAC
    CCCAAGAAGC CAAAAGATAA GGCCGAGGGG GGTGATGGGA AAGCCGAGCC GGAAAAGACC
    CTCGGCTTCC CCAGCACGGA CAGCTTGGAG GTCAGCACAT CCAGCAGCCT GACCCCCGCT
    ATGAGCGTCA GCGCATCCGC GTCCACCAGC CAGGCTTCCA TCTGCAGCTC CCAGGGCATC
    TCGCAGACAG TCAGTGACCT CTCTGCGGAT CCGTTGCCTG CAGGCCTGGA GCTGCCCATC
    CCTGCAGGCT TGTTGGAGCC CCATGTGGTG TCCAGCCAGG AGAGCCTGGA CATTTCCCTG
    TGTAGCACCG GCAGCCTGGG CAGCCTGGGG AGCCTGGGCG AGCCACTGGA CAACGCAGAA
    ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC
    CTTGCAGCGC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TAAGGTCCCG AGCCAAAATT
    GAGAAAATTC GAGCAAGTTT ATTTAACAAC AATGACTTGA TAGGTTTGTC AAGCTTGGAT
    GGTGAGGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACGGTGTC TGTAGTGAAT
    CAGAGTCTAT TCGATACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA
    ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC
    TGTGCCCACT CGGAGCAGAT GCTGAGCCTG ACGCCAGCAA AGCCCATCAA AGTGTCGGAC
    ATTTATCTTA GCAAAGAGCA GATCAACTCC CAGACACCGG GCAACCTCCT CCACCTTTTC
    TTCACCAATG TCCGGCCCCC CAAAAAGGTG CTAGAGGACC AGCTCACCCA GATCCTGAGG
    AAGTACGGTG TGCCGAAGCC CAAGTTTGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG
    CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCGCC CACCAAGGAC
    AAGGCAGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGTG AGAAGAAGCC CACCGTGAAG
    CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT GAAAAGGACC CTGAAAAGTC ACCCACCAAG
    AAACAAGACG TCCCAGAGGA GAAGTACCTG ACCTTAGAAG GATTTCACAA GTTTGTGATC
    GACCGAGCCA AGCAAGACAT CCGCAGCGTC TGGAGAGCGA TTTTATCCTG TGGCTACGAT
    CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCATG CGCACAAGGC CTCCAGGAAG
    TGGACCCTGG AGATGGATGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG
    GCCATCACGC CCGCGCGTCT GCATCCCCAC GAGGTGTATC TGGACCCTGC AGACGCTGCT
    GACCCCAGAG TGGCCTGTCT CTTGAACGTG CCCATCGAGA GCCTGCGCCT GCGCTTCGCT
    CTGCTGCAGT CCCTCAACAC CACGCTCGAG ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC
    CAGACTCCCA TGTACACGCA CAGCATCGCC GCCCTGCTCA AGGAGGCCAA AGGGCTGATC
    TTTTATGACA CAAAGGTGAC CGTCATGAAT CGGGTGCTAA ATGCCACGGT GCAGAGGACG
    GCTGACCATG CAGCACCAGA AATCACTCTG GACCCGCTGG AGATCGTGGG AGGGGAGATC
    AGAGCCTCTG AGAACTCCTA CTTCTGCCAG GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC
    TCCCAGCTTT GCGTCAAACT GGCGAGTGGC GGCGACCCTA CCTATGCCTT CAACATCCGC
    TTCACGGGAG AGGAGGTCCA TGGCACCAGT GGCTCCTTCC GCCACTTCCT GTGGCAGGTG
    TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC
    AACAAGAACA AGGGCAAGTA CATCCTGACC CCCAGCCCCA TCACCTATGG GGAGGAGCAG
    CTGCTGCACT TCCTGGGCCA GTTGCTAGGG ATCGCCATCC GAGCAGATGT CCCCCTCCCC
    CTGGACCTCC TGCCCTCCTT CTGGAAGACA CTGGTGGGCG AGCCCCTGGA CCCTGACCAA
    GACCTGCAGG AAGCAGACGT ACTCACCTAC AATTACGTCA AGAAATTTGA GAGTATCAAC
    GACGAGACGG AGTTGGAGGC CCTGTGTGCT GAGATCGCCT CCCAGCACCT GGCCACTGAG
    AGCCCCGAGG GGCCCAACAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GACCGGCGAG
    GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC ATCCCCGTGG CGTGGGAGAA CAAGGACATC
    TATGCGGCCG CCATCCGGAG CCTGCGGCTG CGTGAGCTAC AGAACGTGGA GTGTGTGACG
    GCCGTGCGCG CGGGCCTTGG GGCCATCATC CCCCTGCAGC TGCTCACCAT GCTCAGCCCA
    CTGGAGATGG AGCTGCGCAC CTGCGGCCTC CCGTACATCA ACCTCGAGTT CCTGAAGGCC
    CACACCATGT ACCAGGTGGG GCTGATGGAG ACCGACCAGC ACATCGAGTT CTTCTGGGGG
    GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAG
    GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGTC CTGACACGGC CCATGTGCCC
    CCATACCCCA TGAAGATTGC CCCCCCAGAT GGCACAGCAG GTTCCCCAGA TTCTCGCTAC
    ATCCGAGTGG AGACCTGTAT GTTCATGATC AAGCTTCCCC AGTACTCCTC GCTGGAAATC
    ATGCTGGAGA AACTACGTTG TGCCATTCAC TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812361.2
    CDS284..13540
    Translation

    Target ORF information:

    RefSeq Version XM_010814059.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814059.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATTG GTGATATACG CGGGGCCTAA CACAAACAGT
    AGGAAGGTTG CTGAGTATGG AGGCAATACA CTGGGATATG GCAGCCGTAG TGTCTTAGGA
    ACTGGTTGGC CGAAAGACTT GGTGAAGGTG GAAGGAGATA CAGTCACCTT CTCCTTTGAA
    ATGAGAAGTG GCCGTGAACA CAACACTCCC GATAAGGCTA TGTGGGGCTT TGCTTGCACA
    GTTCGCGCTC AGGAGTCTTC GGAGGATGTG TCAGGAGGCT TACCCTTTCT GGTAGACCTG
    GCTTTAGGTC TATCTGTGTT AGCTTGTTCC ATGTTAAGAA TCCTATACAA CGGACCAGAA
    ATTACCAAAG AAGAAGAAAC CTGTCAGGAG CTGTTGCGGT CCAAACTTTT ACAAAGGTGC
    CAGTGGCAGG TAGAGGCCAA CGGTGTGATC TCCCCTGCCC TTACTCCAAG CCCATCTCCA
    CTGCCTCTGA CCATCGAGGA AGACAGAGAG TTCACTTACC CGTCTGATGT CCTCGTGCCT
    CCTGTTGGAA ACTACTTTGA CCTGCCCCGG ATCAGGCTGC CTCCAGGAAT CATGATAAAG
    CTCAGGGAAA TTTCTGGGCG TGCTAGACCA CAGTTTAGAC CAAGTATAAA AGAAGTAATT
    CAGCCAGATG TGATGGAGGA AATGGTGGTA TCATGTGTTA TTAAGCACTT GAACTTAGTT
    GATGCACTGC AGTCCCTAAT AAATTTCCAG TATCAAGAAG AACATGCTGA GGAATATGAT
    TTACTGTGTA AAATCATGGG AGAGACATTT AAGAAACTCA ATGCCATGGA GAGACAGCTG
    CAGAGTGTTG CAGAATTGGA ACAGAAATGG CAGAGTGAAG TTGATGATGC CATGCAGGGG
    AAACTGGAGA ACAACATGCC TTTCTTCTAT GATTACCATT TCAATGAGAA CAAAATGAAA
    GAACTAGAAC TTTTGTGTTC AATGAAGGAA GTCTCCTTTG ATGGAAATGA TCTTGAAAAC
    ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA CAAGAAGTGA ATTCTCTTAT TCAGAAACCC
    TCGCACCCAC TGGCTAAAAC GAAGACGTTA GTGAAGAGTC TAATGAACCG AGCTGAGCTG
    TTGCTGCACG TCACCATTGC AGCCCAGTCT GGCCTCACGA GAAGCATCTC AGGGACCCCC
    GCGGAAACAC CGGCTTGTAA ATCAGCCTCT GAAACAAAGG TGATCTCTCA TGCTGTGCGT
    CAGCCTGTTT TCCTTCGCAG CATGTCGGCT CCCTCTGACC TGGAAATGAT CGGTAATGAA
    GATTTGGAAT TTACTAGAGC AAATCAGAGG CGGCGCCACG TGACCAGCCA CCGCAGCAGC
    TCCTTCACAC TCCTGCAGTC ATTGGCCATA GAGGACAGCA GGGACAAGCC CACCTACAGC
    GTCCTGCTGG GGCAGCTGTT TGCTTTCATC GGCACCAACC CTGACCAAGC TGTGTCCAGC
    AGCAGCTTTC TTCTGGCCGC ACAGACGAGA TGGCGGCGGG GAAACACCCG CAAGCAGGCG
    TTGGTGCACA TGCGGGAGCT GCTGACGGCT GCCGTGCGAG TCGGGGGAGT GACACACCTT
    GTGGGTCCAG TGACAATGGT CCTGCAGGGA GGACCCAGAA TTGAAGAACT GACCTGTGGT
    GGGATGGTCG AGCAGGTCCA GGAAGCCTTC GGTGAGACCA TGACCTCTGT GGTGTCTTTG
    TGCGCACGTT ACCCTATCGC ATGTGCAAAT AGCATCGGAC TCTTGTGCAC TATACCTTAT
    ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG AGTGGCCTTG TCCAGCTCAT GGATCGACTA
    TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC TCCAGTGAGA AACAAACCAA GAAGCAGAAG
    GTGGCCACCA TGGCCTGGGC AGCCTTCCAG GTGCTTGCCA ACCGCTGCGT TGAATGGGAA
    AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG CACTCAGGTC TGGCCCGTCA GGTGTCCAGC
    CTTCTCACGA ATCACCTTGC CCGAGCGACC GAGTGCTGTG GCAACCAGGC TGCCGGGAAC
    GATGCCCTCC AGGACGTCCT GAGTCTCCTC AATGATCTCT CGAGGAGTCA TATAGGTAAA
    GCCATCCTGA GCCAGCCAGC TTGTGTGTCC AAACTCCTCT CCCTGCTACT TGACCAACGC
    CCATCCCCAA AGCTGGTTCT TATCATTCTT CAGCTGTGCC GGGCGGCACT CCCGCTGATG
    AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC CCACCCTGGA GCTACTCCGT CCCCTCCCTA
    AACAGCGAGC AGGAGGATCC CAGCGACCCA GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA
    AAGCTGGCAG ACTACGTGGT CCCAGGATGT CAGACAGTCC TTTCTCCAAC CGCTTCCGAA
    CCTGACACCA CATTGACAAA AACCAGCCCC AAGAATTCCT TAAAAGGCGA TAAAGATCCT
    GGAGAAGAGA GTGAAGCAGT GGATGGCAAG CTCTCTATCT TCATCCACAA GCGGGAAGAC
    CAGTCGTCCC ATGAAGTTCT GCAGCCATTA CTAAGTAGCT CAGAAGGACG GCCCTTCCGA
    CTTGGTACTG GTGCCAACAT GGAGAAAGTT GTGAAAATGG ACCGAGACAT GACGAAGGGT
    GGCTGCTGTG AGGTGATTAC GGAAGAGGCG GCAGCTGCTC TGCGAAAAGC GACCAAGTGG
    GCACAGTCAG GCCTCATTGT CAGTGTTGGG CCACCCGTGG AGTCCCTCAA CCCAGAGGCC
    GTGAGCGGGC TGTCCACAGG GGACAAGAAG AAGACTGCCC AAACCTCCAT TTGCCGAGAG
    AGAAACTCTG AACTCGCCAG GACCGACCCC GTCCGACCTT TCATCAGTGG GCACGTGGCA
    AACAGCATGG CTGCTGAGGT GATCGCCTTG CTTCATAGCT TGCTGATGGC ACCTGAGTCA
    AATGCTGCTC AGATATGGAC CACGACAGCA GAGAAGGTTC TGTCTCGTGC ACTGATGTAC
    ATTCCACAGT TGGGGAAATA TGCAGAAAGC ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA
    AAGCTTGCCA AACTTCAGAG AATTGCCCGC CAGGCTGTGG CTGCACTCTG TGCACTTGGA
    GGCTTCAAAG AGACAATCAA GATCGGGTCT GAGGTCCAGG TTTTAGGTAG AGGAATATCA
    GGAAGCATCG GAGTGGTGGC TTCTATCAAC GAACAGGAAG GTATAGCTAC GGTCAGATTC
    CCACCCATAG ACTGTAGAAA GACTTCGCAA GCATCAGACA CACTGACTAT TCCATTGTCT
    AGACTTTGTG TTCCAAGATC AGAGGCATTG CCTCTTCATA AACTGTCCAT TACTGAGAAG
    GTAGTACAGG CAGTCCAGTC CATGTTGCTT CCTCAGGAGG GAAGTCTCTC TATTCATACC
    TCACTTCCTG CAGCAGGAGA TGGGTCAGCT CCTGTGATGG CAGTCGTTCG GCTCCTGGCT
    GAAATAAGAA CAAGAGCATG CCTGGTCATG GCCCAGCTTT TAGAAGACAG CTTATTTTGT
    GAAGAATTCA TACAGCAGTG TCCAGCAGCT GTTGAAGTTC TTAACCTAGT AGCCCAGGAA
    TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG GAAGTGCAGT GTGAACGGCT GAGGATGCTC
    TACCGGGACT GTGCTCGGCC CCCGCCGCCT CCGCTGCAGG CTGACCGGAG ACAGCCCAAA
    GAGATTACGT GGAGCCCCTC ACGAGTGTTT CCACCTGTCC GAGCCTGCAT GTTCTCCTCG
    CACCTCACTT CTGTCACCTT CCTAGCAGAC CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC
    ACATTTATCT ACGCCACCTC ACCACTGCCC GTTCAGGCCC CGTCTTTCTA CTGGGAAATT
    GAAATTGTTT CCTATGGAGA TACAGATGAT GATACTGGAC CAATAGTTTC CTTTGGCTTT
    GCTACAGAAG CAGAGAAAAG AGATGGGGCA TGGACTAATC CAGTGGGCAC CTGTCTTTTC
    CATAACAATG GCCGAGCTGT GCATTACAAT GGCTCCAGTT TATTACAGTG GAAGAGCGTT
    CGATTAGACG TAACTCTCTC TCCTGGTGAC GTGGCAGGGA TTGGCTGGGA GAGAACCGAG
    GGGACGCCTC CTCCACCCGG GCAGCCTGCC AAGGGCCGTG TGTACTTCAC ATACTGTGGG
    CAGCGCCTCT CACCGTATCT CGAAGATGTC TCGGGTGGGA TGTGGCCGGT GGTTCATATT
    CAGAAAAAGA ACACCAAGAC TCGCGCTAAT TTTGGCTCCC GCCCATTTGC CTACGCTGAA
    GGGCAAGCCC ACCGCAATGC TGCCGACCTG TGCACTGACC TGGCCGAGGA AATTAGCGCT
    AACTTCGAGG CCCTGCCCTT TGCCATGGCC TCTGACAGTG ATAACGATGC TGGCACCAGT
    ATTGCGTCAG ACCCGGGCAC TCATGGGCCA CCATGCCGAA TTGCTGCCGT AGCCACTGCT
    CAGCAACAAT ATGATAGTGA TACTTCCTGT CATTATAAAG TGGAGCTCAG CTATGAGAAT
    TTCATCACCT CTGGGCCAGA CCCTCACCCA CCCCCCATTG CAGATGACGA AAGTGATGAT
    GATGATGACG ATGACATCCC ACAGGAGGAT CACTATGCCC TGCTGGTGAA AGCCTGGGAG
    ACCAAGGTTT TTCCTACCAT CCGAAGACGG TTCCGCAACG AAGCAGAGAG GAAATCAGGA
    CTGGACCAGA TAAAGGGTGC TTTACAACTA GGCATGGTGG ATATTGCCCG GCAGACAGTT
    GAATTCCTCT ACGAGGAGAA TGGTGGCATC CCAAGAGACC TTTATCTTCC CACCATTGAA
    GACATTAAAG ATGAAGCGAA CAAGTTCACA ATTGATAAAG TTCGAAAAGG TCTCACGGTA
    GTAACACGCT CTCCAGACAG CAATAACGTA GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA
    AAATTTGCTA TCCGAGGGAT GCTGAAAACC TTTGGGCTTC ATGGAGTTGT CTTAGATGTT
    GATTCAGTTA ATGAACTAGT TCAGGTAGAA ACGTACCTCC GAAGTGAAGG TGTGCTGGTC
    CGATACTGGT ACCCTATTGA TATGTTGGAA AGGCCCCCCG CAGGCTACAG AAGGACTGCC
    ACCAATGGGC TCGTCACACT GGACAATACC AACCTTCAAA TTCACAGGGA GCTGCTGCGC
    TGCGAGGCCG CGCTGGCCAG GCTGTACTGC CGCATGGCCC TGCTCAACAT CTTCGCCCCC
    AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC CACATCCCCG CCATCCGGGA CATTACCCTG
    GAGCACCTGC AGCTGCTGTC CAATCAGCTC CTCGCTCCTC CCCTCCCAGA TGGCACCATC
    AGCTCCAGCT CCATCCTCCT GGCGCAGTCC CTGCAGCACT GTGTCCATTC CCAGAACTGT
    TCGGCCACAG ACCTCTTCTA CCAGGGCAGC TCCCAGACGG TGCGAGAGTG GCTCAACGTG
    GCCATCACGC GGACCCTGCA CCAGGGCGAG GAAAGCCTTC TGGAGCTGAC CAAACAGATC
    TGCTCTTTCC TGCAGACAGC ACCAGAACAG TTCCCCTCCG AAGAGTTTCC AATTTCCGAG
    TCCAAAGTCA ACATGGACGT CAATTTCCCC GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA
    GAAAGTCAGT CTGGATTCCG CAAAGATTCC TCTCTGTACA AGGCACCATG GGCGCGTGTG
    CTGGTGTACG GGCTAGGCCA CAAAGTGAAG CGAAATGGCC AGCTGAACCT CATTGAGGCC
    GTCTGTTACC CGCGGGATGC GTCTCCGGCC AACACAGGGC TCACACCACC TCCTACCACC
    AACCAGTACC CTTCTGTGAT CCTCTCCACA GACAAAGTCC ACATCAAACT GGGGGTATCT
    CCCCCACCCG GAGCCGTGCT GGTGTTACAC TCCCTGCCTC TCGAGTTCCC CCTGGCGATG
    GCCTTCACCG AGCAGCTACT GTCCTGGAAA TCAGAGGATG GCGAAGGTAG AGCTGAAGAT
    GAGCCTGACA CCATCCCGAC GTCCGTCCTC CTGCAAGTGG TGGAGCTGCT TGGCAACTTC
    CTCTGGACCA CAGACATGGC GGCCTGCGTG AAGGAGCTGG TTTTCCACCT CCTGGCAGAG
    CTCCTGCGCA CGGTGCACAG CCTAGAGCAG AGGAAGCACC CAGCCGGCCT GTCCTCATCC
    ATCGCCCTCC AGCTGAACCC CTGCCTGGCC ATGCTGATGG CCCTGCAGTC GGAGCTTCAC
    AAGCTGTACG ACGAGGAGAC GCAGAGCTGG GTCTCGGGCA GTGCATGCGG AGGCTCAGGA
    GCCACGGCGG CTGCTGACCA GGGCCGGTTC TCGACGTATT TCCACGCACT CATGGAGGGC
    TGCCTGGCGG TGGCCGAGGT GACCCTGCCC ACCAACATGA GTGTCACCGC CAGCGGGGTG
    ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC TCGTCCTCCT CCTCATCGTC CTCCCCAGGA
    CAGACTCCAC AGAGCCCCAG CCTCCTGTCC AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA
    AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC TCACGCTCTG TGGACTCGGA CCCCGCCGTG
    CTGAGCATCG GGGGCAGCAA GCCCGAGGAC ATGCTGTGGT TCCATCGTGC TCTGACGTTG
    CTCATCATCC TCCGCCACCT CACCAGGAAG GACCCACAGG GGCTAGGCGT GACAAGCGAC
    GCCATCGCCG ACGCCTGCCA GGCCCTGGTG GGCCCCACCG CCCACAGCCG CTTGCTGGTG
    ATCTCTGGGA TCCCCACCCA CCTGGACGAG GGCATCGTCA GGGGTGCCAT CCGCAAGGCC
    TGCAATGCCC ATGGCGGGGT GTTCAAGGAT GAGATCTACA TCCCCCTGCA GGACGAAGAC
    CCCAAGAAGC CAAAAGATAA GGCCGAGGGG GGTGATGGGA AAGCCGAGCC GGAAAAGACC
    CTCGGCTTCC CCAGCACGGA CAGCTTGGAG GTCAGCACAT CCAGCAGCCT GACCCCCGCT
    ATGAGCGTCA GCGCATCCGC GTCCACCAGC CAGGCTTCCA TCTGCAGCTC CCAGGGCATC
    TCGCAGACAG TCAGTGACCT CTCTGCGGAT CCGTTGCCTG CAGGCCTGGA GCTGCCCATC
    CCTGCAGGCT TGTTGGAGCC CCATGTGGTG TCCAGCCAGG AGAGCCTGGA CATTTCCCTG
    TGTAGCACCG GCAGCCTGGG CAGCCTGGGG AGCCTGGGCG AGCCACTGGA CAACGCAGAA
    ACGGCCTCAG TGTCAGACAT GGGCTCCATG TACACAGTCA CGTCCCTGGA CAACCAGCCC
    CTTGCAGCGC GCCCCATCAA AGGCTTCGCG GTCGTGGAGA TAAGGTCCCG AGCCAAAATT
    GAGAAAATTC GAGCAAGTTT ATTTAACAAC AATGACTTGA TAGGTTTGTC AAGCTTGGAT
    GGTGAGGATG AATTGATGGA AATGTCAACG GAAGAGATCC TCACGGTGTC TGTAGTGAAT
    CAGAGTCTAT TCGATACGCA GGGGAGCCCA GGGTTAGAAG ATTATTTCAA TGATAAGTCA
    ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC AGAGAGGTGC TGACGGAGAT ATTTAAGAGC
    TGTGCCCACT CGGAGCAGAT GCTGAGCCTG ACGCCAGCAA AGCCCATCAA AGTGTCGGAC
    ATTTATCTTA GCAAAGAGCA GATCAACTCC CAGACACCGG GCAACCTCCT CCACCTTTTC
    TTCACCAATG TCCGGCCCCC CAAAAAGGTG CTAGAGGACC AGCTCACCCA GATCCTGAGG
    AAGTACGGTG TGCCGAAGCC CAAGTTTGAC AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG
    CAGCACCCCG GGAAGGTGGT GAGCACCAAG CGGCCCATCA CCAAGCCGCC CACCAAGGAC
    AAGGCAGTGC TCAACAGCGT CAGCAGGACT GCCTTGAGTG AGAAGAAGCC CACCGTGAAG
    CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT GAAAAGGACC CTGAAAAGTC ACCCACCAAG
    AAACAAGACG TCCCAGAGGA GAAGTACCTG ACCTTAGAAG GATTTCACAA GTTTGTGATC
    GACCGAGCCA AGCAAGACAT CCGCAGCGTC TGGAGAGCGA TTTTATCCTG TGGCTACGAT
    CTTCATTTTG AGAGGTGTGC CTGCATCGAC GTCCGGCATG CGCACAAGGC CTCCAGGAAG
    TGGACCCTGG AGATGGATGT GGCGCTCGTG CAGTACATCA ACCGGCTGTG CCGCCACCTG
    GCCATCACGC CCGCGCGTCT GCATCCCCAC GAGGTGTATC TGGACCCTGC AGACGCTGCT
    GACCCCAGAG TGGCCTGTCT CTTGAACGTG CCCATCGAGA GCCTGCGCCT GCGCTTCGCT
    CTGCTGCAGT CCCTCAACAC CACGCTCGAG ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC
    CAGACTCCCA TGTACACGCA CAGCATCGCC GCCCTGCTCA AGGAGGCCAA AGGGCTGATC
    TTTTATGACA CAAAGGTGAC CGTCATGAAT CGGGTGCTAA ATGCCACGGT GCAGAGGACG
    GCTGACCATG CAGCACCAGA AATCACTCTG GACCCGCTGG AGATCGTGGG AGGGGAGATC
    AGAGCCTCTG AGAACTCCTA CTTCTGCCAG GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC
    TCCCAGCTTT GCGTCAAACT GGCGAGTGGC GGCGACCCTA CCTATGCCTT CAACATCCGC
    TTCACGGGAG AGGAGGTCCA TGGCACCAGT GGCTCCTTCC GCCACTTCCT GTGGCAGGTG
    TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC
    AACAAGAACA AGGGCAAGTA CATCCTGACC CCCAGCCCCA TCACCTATGG GGAGGAGCAG
    CTGCTGCACT TCCTGGGCCA GTTGCTAGGG ATCGCCATCC GAGCAGATGT CCCCCTCCCC
    CTGGACCTCC TGCCCTCCTT CTGGAAGACA CTGGTGGGCG AGCCCCTGGA CCCTGACCAA
    GACCTGCAGG AAGCAGACGT ACTCACCTAC AATTACGTCA AGAAATTTGA GAGTATCAAC
    GACGAGACGG AGTTGGAGGC CCTGTGTGCT GAGATCGCCT CCCAGCACCT GGCCACTGAG
    AGCCCCGAGG GGCCCAACAA GCCCTGCTGC CGGTTCACCT ACCTGACCAT GACCGGCGAG
    GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC ATCCCCGTGG CGTGGGAGAA CAAGGACATC
    TATGCGGCCG CCATCCGGAG CCTGCGGCTG CGTGAGCTAC AGAACGTGGA GTGTGTGACG
    GCCGTGCGCG CGGGCCTTGG GGCCATCATC CCCCTGCAGC TGCTCACCAT GCTCAGCCCA
    CTGGAGATGG AGCTGCGCAC CTGCGGCCTC CCGTACATCA ACCTCGAGTT CCTGAAGGCC
    CACACCATGT ACCAGGTGGG GCTGATGGAG ACCGACCAGC ACATCGAGTT CTTCTGGGGG
    GCACTGGAGA TGTTTGCCCA GGAGGAGCTG TGCAAGTTCA TCAAGTTTGC CTGCAACCAG
    GAGCGCATCC CGTTCACCTG CCCCTGCAAA GACGGGGGTC CTGACACGGC CCATGTGCCC
    CCATACCCCA TGAAGATTGC CCCCCCAGAT GGCACAGCAG GTTCCCCAGA TTCTCGCTAC
    ATCCGAGTGG AGACCTGTAT GTTCATGATC AAGCTTCCCC AGTACTCCTC GCTGGAAATC
    ATGCTGGAGA AACTACGTTG TGCCATTCAC TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224641
    Clone ID Related Accession (Same CDS sequence) XM_010814061.3
    Accession Version XM_010814061.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010814061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAACGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812363.1
    CDS284..13534
    Translation

    Target ORF information:

    RefSeq Version XM_010814061.3
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814061.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAACGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224642
    Clone ID Related Accession (Same CDS sequence) XM_015475528.2
    Accession Version XM_015475528.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015475528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
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    7141
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    7261
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    7381
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    7501
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    7681
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    7921
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    8401
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    8641
    8701
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAACGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331014.1
    CDS229..11877
    Translation

    Target ORF information:

    RefSeq Version XM_015475528.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475528.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAACGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224643
    Clone ID Related Accession (Same CDS sequence) XM_024977764.1
    Accession Version XM_024977764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    10501
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    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGCAGGA CTGCCTTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024833532.1
    CDS284..11626
    Translation

    Target ORF information:

    RefSeq Version XM_024977764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGTTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGTACAGGA GTTAAAGGTC
    AGTATTTTGG CTCTTGCTAC CCAAATCCTG ACTGGATGTG ATGAAGTGTT GGAAATGCTA
    CAGCAGGTCA CAACTGCCCT CATAAATAGT GACATACCCG ACCGGGAGCA GAGGTTAAAA
    GGCTTGGAAC AAGTTACTAA GGCTACTATG CTTGGTCACC TTCTCCCAGT GTTACTCACT
    TCCCTGATGC ATCCAAATTT ACAAACTTTG ACCATGGCCG ATGCCCTGAT GCCTCAGCTA
    GTGCAGCTGG TACTCTATAC CAGTCAGACG GCTTTGCTGC TTAAAACCCA GTGTCCTGTT
    TTTGCTGAGG TGGGCTGTTC CCCATGTGGT GCATCAGACC AGAAGGGCAG GCTTTTGCCG
    GATGAGAGAA TGCTGGAAGA GAAGGAGGAG CCAGGGTTTC TCACTGGTTT AAAGATTCCT
    GCTCCATGGG CTGCTGGGAA GACTGTGGAA ACAGTCCACC CTGTCAGAGA CAACTATAAA
    TTTAAAGAAA CTGTCCACAT CCCCGGAGCT CGCTGCCTGT ACCTTAGATT TGATAGCAGA
    TGCTCTTCGC AATATGACTA CGACAAATCT AACAGTATAT TTTCTGTTTT GAAGTTGGTG
    ATATACGCGG GGCCTAACAC AAACAGTAGG AAGGTTGCTG AGTATGGAGG CAATACACTG
    GGATATGGCA GCCGTAGTGT CTTAGGAACT GGTTGGCCGA AAGACTTGGT GAAGGTGGAA
    GGAGATACAG TCACCTTCTC CTTTGAAATG AGAAGTGGCC GTGAACACAA CACTCCCGAT
    AAGGCTATGT GGGGCTTTGC TTGCACAGTT CGCGCTCAGG AGTCTTCGGA GGATGTGTCA
    GGAGGCTTAC CCTTTCTGGT AGACCTGGCT TTAGGTCTAT CTGTGTTAGC TTGTTCCATG
    TTAAGAATCC TATACAACGG ACCAGAAATT ACCAAAGAAG AAGAAACCTG TCAGGAGCTG
    TTGCGGTCCA AACTTTTACA AAGGTGCCAG TGGCAGGTAG AGGCCAACGG TGTGATCTCC
    CCTGCCCTTA CTCCAAGCCC ATCTCCACTG CCTCTGACCA TCGAGGAAGA CAGAGAGTTC
    ACTTACCCGT CTGATGTCCT CGTGCCTCCT GTTGGAAACT ACTTTGACCT GCCCCGGATC
    AGGCTGCCTC CAGGAATCAT GATAAAGCTC AGGGAAATTT CTGGGCGTGC TAGACCACAG
    TTTAGACCAA GTATAAAAGA AGTAATTCAG CCAGATGTGA TGGAGGAAAT GGTGGTATCA
    TGTGTTATTA AGCACTTGAA CTTAGTTGAT GCACTGCAGT CCCTAATAAA TTTCCAGTAT
    CAAGAAGAAC ATGCTGAGGA ATATGATTTA CTGTGTAAAA TCATGGGAGA GACATTTAAG
    AAACTCAATG CCATGGAGAG ACAGCTGCAG AGTGTTGCAG AATTGGAACA GAAATGGCAG
    AGTGAAGTTG ATGATGCCAT GCAGGGGAAA CTGGAGAACA ACATGCCTTT CTTCTATGAT
    TACCATTTCA ATGAGAACAA AATGAAAGAA CTAGAACTTT TGTGTTCAAT GAAGGAAGTC
    TCCTTTGATG GAAATGATCT TGAAAACATG GTGCTATCTC TGAGGGAGAA GTTCCTACAA
    GAAGTGAATT CTCTTATTCA GAAACCCTCG CACCCACTGG CTAAAACGAA GACGTTAGTG
    AAGAGTCTAA TGAACCGAGC TGAGCTGTTG CTGCACGTCA CCATTGCAGC CCAGTCTGGC
    CTCACGAGAA GCATCTCAGG GACCCCCGCG GAAACACCGG CTTGTAAATC AGCCTCTGAA
    ACAAAGGTGA TCTCTCATGC TGTGCGTCAG CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC
    TCTGACCTGG AAATGATCGG TAATGAAGAT TTGGAATTTA CTAGAGCAAA TCAGAGGCGG
    CGCCACGTGA CCAGCCACCG CAGCAGCTCC TTCACACTCC TGCAGTCATT GGCCATAGAG
    GACAGCAGGG ACAAGCCCAC CTACAGCGTC CTGCTGGGGC AGCTGTTTGC TTTCATCGGC
    ACCAACCCTG ACCAAGCTGT GTCCAGCAGC AGCTTTCTTC TGGCCGCACA GACGAGATGG
    CGGCGGGGAA ACACCCGCAA GCAGGCGTTG GTGCACATGC GGGAGCTGCT GACGGCTGCC
    GTGCGAGTCG GGGGAGTGAC ACACCTTGTG GGTCCAGTGA CAATGGTCCT GCAGGGAGGA
    CCCAGAATTG AAGAACTGAC CTGTGGTGGG ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT
    GAGACCATGA CCTCTGTGGT GTCTTTGTGC GCACGTTACC CTATCGCATG TGCAAATAGC
    ATCGGACTCT TGTGCACTAT ACCTTATACC AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT
    GGCCTTGTCC AGCTCATGGA TCGACTATGC AGCTTGAGCA GTCAGACTGA GTCCAGCTCC
    AGTGAGAAAC AAACCAAGAA GCAGAAGGTG GCCACCATGG CCTGGGCAGC CTTCCAGGTG
    CTTGCCAACC GCTGCGTTGA ATGGGAAAAA GAAGAAGGCG GCTCCACAGA GGCTGTGCAC
    TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT CTCACGAATC ACCTTGCCCG AGCGACCGAG
    TGCTGTGGCA ACCAGGCTGC CGGGAACGAT GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT
    GATCTCTCGA GGAGTCATAT AGGTAAAGCC ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA
    CTCCTCTCCC TGCTACTTGA CCAACGCCCA TCCCCAAAGC TGGTTCTTAT CATTCTTCAG
    CTGTGCCGGG CGGCACTCCC GCTGATGAGC GTAGAAGACT GTGGGAACGT GGAGCTCCCA
    CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC AGCGAGCAGG AGGATCCCAG CGACCCAGCG
    TCCAAGATCG CCTCGCTGCT CTTGGCAAAG CTGGCAGACT ACGTGGTCCC AGGATGTCAG
    ACAGTCCTTT CTCCAACCGC TTCCGAACCT GACACCACAT TGACAAAAAC CAGCCCCAAG
    AATTCCTTAA AAGGCGATAA AGATCCTGGA GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC
    TCTATCTTCA TCCACAAGCG GGAAGACCAG TCGTCCCATG AAGTTCTGCA GCCATTACTA
    AGTAGCTCAG AAGGACGGCC CTTCCGACTT GGTACTGGTG CCAACATGGA GAAAGTTGTG
    AAAATGGACC GAGACATGAC GAAGGGTGGC TGCTGTGAGG TGATTACGGA AGAGGCGGCA
    GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA CAGTCAGGCC TCATTGTCAG TGTTGGGCCA
    CCCGTGGAGT CCCTCAACCC AGAGGCCGTG AGCGGGCTGT CCACAGGGGA CAAGAAGAAG
    ACTGCCCAAA CCTCCATTTG CCGAGAGAGA AACTCTGAAC TCGCCAGGAC CGACCCCGTC
    CGACCTTTCA TCAGTGGGCA CGTGGCAAAC AGCATGGCTG CTGAGGTGAT CGCCTTGCTT
    CATAGCTTGC TGATGGCACC TGAGTCAAAT GCTGCTCAGA TATGGACCAC GACAGCAGAG
    AAGGTTCTGT CTCGTGCACT GATGTACATT CCACAGTTGG GGAAATATGC AGAAAGCATT
    CTGGAAAATG GCAGCAGCAG TGGCAGAAAG CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG
    GCTGTGGCTG CACTCTGTGC ACTTGGAGGC TTCAAAGAGA CAATCAAGAT CGGGTCTGAG
    GTCCAGGTTT TAGGTAGAGG AATATCAGGA AGCATCGGAG TGGTGGCTTC TATCAACGAA
    CAGGAAGGTA TAGCTACGGT CAGATTCCCA CCCATAGACT GTAGAAAGAC TTCGCAAGCA
    TCAGACACAC TGACTATTCC ATTGTCTAGA CTTTGTGTTC CAAGATCAGA GGCATTGCCT
    CTTCATAAAC TGTCCATTAC TGAGAAGGTA GTACAGGCAG TCCAGTCCAT GTTGCTTCCT
    CAGGAGGGAA GTCTCTCTAT TCATACCTCA CTTCCTGCAG CAGGAGATGG GTCAGCTCCT
    GTGATGGCAG TCGTTCGGCT CCTGGCTGAA ATAAGAACAA GAGCATGCCT GGTCATGGCC
    CAGCTTTTAG AAGACAGCTT ATTTTGTGAA GAATTCATAC AGCAGTGTCC AGCAGCTGTT
    GAAGTTCTTA ACCTAGTAGC CCAGGAATGC AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA
    GTGCAGTGTG AACGGCTGAG GATGCTCTAC CGGGACTGTG CTCGGCCCCC GCCGCCTCCG
    CTGCAGGCTG ACCGGAGACA GCCCAAAGAG ATTACGTGGA GCCCCTCACG AGTGTTTCCA
    CCTGTCCGAG CCTGCATGTT CTCCTCGCAC CTCACTTCTG TCACCTTCCT AGCAGACCCC
    AGTGCAGGGG GCGGTCTGCC CAGGGGCACA TTTATCTACG CCACCTCACC ACTGCCCGTT
    CAGGCCCCGT CTTTCTACTG GGAAATTGAA ATTGTTTCCT ATGGAGATAC AGATGATGAT
    ACTGGACCAA TAGTTTCCTT TGGCTTTGCT ACAGAAGCAG AGAAAAGAGA TGGGGCATGG
    ACTAATCCAG TGGGCACCTG TCTTTTCCAT AACAATGGCC GAGCTGTGCA TTACAATGGC
    TCCAGTTTAT TACAGTGGAA GAGCGTTCGA TTAGACGTAA CTCTCTCTCC TGGTGACGTG
    GCAGGGATTG GCTGGGAGAG AACCGAGGGG ACGCCTCCTC CACCCGGGCA GCCTGCCAAG
    GGCCGTGTGT ACTTCACATA CTGTGGGCAG CGCCTCTCAC CGTATCTCGA AGATGTCTCG
    GGTGGGATGT GGCCGGTGGT TCATATTCAG AAAAAGAACA CCAAGACTCG CGCTAATTTT
    GGCTCCCGCC CATTTGCCTA CGCTGAAGGG CAAGCCCACC GCAATGCTGC CGACCTGTGC
    ACTGACCTGG CCGAGGAAAT TAGCGCTAAC TTCGAGGCCC TGCCCTTTGC CATGGCCTCT
    GACAGTGATA ACGATGCTGG CACCAGTATT GCGTCAGACC CGGGCACTCA TGGGCCACCA
    TGCCGAATTG CTGCCGTAGC CACTGCTCAG CAACAATATG ATAGTGATAC TTCCTGTCAT
    TATAAAGTGG AGCTCAGCTA TGAGAATTTC ATCACCTCTG GGCCAGACCC TCACCCACCC
    CCCATTGCAG ATGACGAAAG TGATGATGAT GATGACGATG ACATCCCACA GGAGGATCAC
    TATGCCCTGC TGGTGAAAGC CTGGGAGACC AAGGTTTTTC CTACCATCCG AAGACGGTTC
    CGCAACGAAG CAGAGAGGAA ATCAGGACTG GACCAGATAA AGGGTGCTTT ACAACTAGGC
    ATGGTGGATA TTGCCCGGCA GACAGTTGAA TTCCTCTACG AGGAGAATGG TGGCATCCCA
    AGAGACCTTT ATCTTCCCAC CATTGAAGAC ATTAAAGATG AAGCGAACAA GTTCACAATT
    GATAAAGTTC GAAAAGGTCT CACGGTAGTA ACACGCTCTC CAGACAGCAA TAACGTAGCC
    AGCAGCACTG TTGGAACTGC CCTGCCAAAA TTTGCTATCC GAGGGATGCT GAAAACCTTT
    GGGCTTCATG GAGTTGTCTT AGATGTTGAT TCAGTTAATG AACTAGTTCA GGTAGAAACG
    TACCTCCGAA GTGAAGGTGT GCTGGTCCGA TACTGGTACC CTATTGATAT GTTGGAAAGG
    CCCCCCGCAG GCTACAGAAG GACTGCCACC AATGGGCTCG TCACACTGGA CAATACCAAC
    CTTCAAATTC ACAGGGAGCT GCTGCGCTGC GAGGCCGCGC TGGCCAGGCT GTACTGCCGC
    ATGGCCCTGC TCAACATCTT CGCCCCCAAG CTGCCTCACC TGTTTACCCG CCTCTTCCAC
    ATCCCCGCCA TCCGGGACAT TACCCTGGAG CACCTGCAGC TGCTGTCCAA TCAGCTCCTC
    GCTCCTCCCC TCCCAGATGG CACCATCAGC TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG
    CAGCACTGTG TCCATTCCCA GAACTGTTCG GCCACAGACC TCTTCTACCA GGGCAGCTCC
    CAGACGGTGC GAGAGTGGCT CAACGTGGCC ATCACGCGGA CCCTGCACCA GGGCGAGGAA
    AGCCTTCTGG AGCTGACCAA ACAGATCTGC TCTTTCCTGC AGACAGCACC AGAACAGTTC
    CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC AAAGTCAACA TGGACGTCAA TTTCCCCGGG
    GCAGCTTTCG TCGTCGTATC TTGCAAAGAA AGTCAGTCTG GATTCCGCAA AGATTCCTCT
    CTGTACAAGG CACCATGGGC GCGTGTGCTG GTGTACGGGC TAGGCCACAA AGTGAAGCGA
    AATGGCCAGC TGAACCTCAT TGAGGCCGTC TGTTACCCGC GGGATGCGTC TCCGGCCAAC
    ACAGGGCTCA CACCACCTCC TACCACCAAC CAGTACCCTT CTGTGATCCT CTCCACAGAC
    AAAGTCCACA TCAAACTGGG GGTATCTCCC CCACCCGGAG CCGTGCTGGT GTTACACTCC
    CTGCCTCTCG AGTTCCCCCT GGCGATGGCC TTCACCGAGC AGCTACTGTC CTGGAAATCA
    GAGGATGGCG AAGGTAGAGC TGAAGATGAG CCTGACACCA TCCCGACGTC CGTCCTCCTG
    CAAGTGGTGG AGCTGCTTGG CAACTTCCTC TGGACCACAG ACATGGCGGC CTGCGTGAAG
    GAGCTGGTTT TCCACCTCCT GGCAGAGCTC CTGCGCACGG TGCACAGCCT AGAGCAGAGG
    AAGCACCCAG CCGGCCTGTC CTCATCCATC GCCCTCCAGC TGAACCCCTG CCTGGCCATG
    CTGATGGCCC TGCAGTCGGA GCTTCACAAG CTGTACGACG AGGAGACGCA GAGCTGGGTC
    TCGGGCAGTG CATGCGGAGG CTCAGGAGCC ACGGCGGCTG CTGACCAGGG CCGGTTCTCG
    ACGTATTTCC ACGCACTCAT GGAGGGCTGC CTGGCGGTGG CCGAGGTGAC CCTGCCCACC
    AACATGAGTG TCACCGCCAG CGGGGTGACG TCAGCAACTG CCCCAAACCT CAGCGACTCG
    TCCTCCTCCT CATCGTCCTC CCCAGGACAG ACTCCACAGA GCCCCAGCCT CCTGTCCAAG
    AGGAAGAAAG TCAAGATGAA GCGGGAAAAG GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA
    CGCTCTGTGG ACTCGGACCC CGCCGTGCTG AGCATCGGGG GCAGCAAGCC CGAGGACATG
    CTGTGGTTCC ATCGTGCTCT GACGTTGCTC ATCATCCTCC GCCACCTCAC CAGGAAGGAC
    CCACAGGGGC TAGGCGTGAC AAGCGACGCC ATCGCCGACG CCTGCCAGGC CCTGGTGGGC
    CCCACCGCCC ACAGCCGCTT GCTGGTGATC TCTGGGATCC CCACCCACCT GGACGAGGGC
    ATCGTCAGGG GTGCCATCCG CAAGGCCTGC AATGCCCATG GCGGGGTGTT CAAGGATGAG
    ATCTACATCC CCCTGCAGGA CGAAGACCCC AAGAAGCCAA AAGATAAGGC CGAGGGGGGT
    GATGGGAAAG CCGAGCCGGA AAAGACCCTC GGCTTCCCCA GCACGGACAG CTTGGAGGTC
    AGCACATCCA GCAGCCTGAC CCCCGCTATG AGCGTCAGCG CATCCGCGTC CACCAGCCAG
    GCTTCCATCT GCAGCTCCCA GGGCATCTCG CAGACAGTCA GTGACCTCTC TGCGGATCCG
    TTGCCTGCAG GCCTGGAGCT GCCCATCCCT GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC
    AGCCAGGAGA GCCTGGACAT TTCCCTGTGT AGCACCGGCA GCCTGGGCAG CCTGGGGAGC
    CTGGGCGAGC CACTGGACAA CGCAGAAACG GCCTCAGTGT CAGACATGGG CTCCATGTAC
    ACAGTCACGT CCCTGGACAA CCAGCCCCTT GCAGCGCGCC CCATCAAAGG CTTCGCGGTC
    GTGGAGATAA GGTCCCGAGC CAAAATTGAG AAAATTCGAG CAAGTTTATT TAACAACAAT
    GACTTGATAG GTTTGTCAAG CTTGGATGGT GAGGATGAAT TGATGGAAAT GTCAACGGAA
    GAGATCCTCA CGGTGTCTGT AGTGAATCAG AGTCTATTCG ATACGCAGGG GAGCCCAGGG
    TTAGAAGATT ATTTCAATGA TAAGTCAATT AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA
    GAGGTGCTGA CGGAGATATT TAAGAGCTGT GCCCACTCGG AGCAGATGCT GAGCCTGACG
    CCAGCAAAGC CCATCAAAGT GTCGGACATT TATCTTAGCA AAGAGCAGAT CAACTCCCAG
    ACACCGGGCA ACCTCCTCCA CCTTTTCTTC ACCAATGTCC GGCCCCCCAA AAAGGTGCTA
    GAGGACCAGC TCACCCAGAT CCTGAGGAAG TACGGTGTGC CGAAGCCCAA GTTTGACAAG
    AGCAAGTACA GCAAGGCGGG GAAGGAGCAG CACCCCGGGA AGGTGCAGGA CTGCCTTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52727
    Clone ID Related Accession (Same CDS sequence) XM_010823423.2 , XM_010814061.2
    Accession Version XM_010823423.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2641
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    9781
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    10261
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAATGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010821725.1
    CDS182..13432
    Translation

    Target ORF information:

    RefSeq Version XM_010823423.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010823423.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAATGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52727
    Clone ID Related Accession (Same CDS sequence) XM_010823423.2 , XM_010814061.2
    Accession Version XM_010814061.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814061.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3601
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    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
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    7381
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    7501
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    7621
    7681
    7741
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    7921
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    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAATGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812363.1
    CDS182..13432
    Translation

    Target ORF information:

    RefSeq Version XM_010814061.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814061.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCGT CGGCGGCCGC GGCGGCTGCG GCGGCTGCGG CCGCGGACTC CGCGCAGTGG 
    CTCTCGGTGA AGGAGGAGAC CATCTTCCTG CACGACGGGC TGATCCGGGT CACCGACCTG
    GCCGAGCTGC CCAGCGAGAT CCTCGGGGCC CCGGAGGCCG CCGACACCGA CTTAGAGATT
    TTAACATTTG AGACCAAGAA CCCAACGGAA TTAGCAGAAC GTCTGCGCTC TGTTTGTGGG
    AATCAGAGCA ACGCCTATGC CCGCCTGCTG GAATACCGCC TGAATGCCTT GCGAGGACTG
    TGGAATGCCC AGCGCCAGCT GGCCTTGGAA GAACAGCATG AAAGGGAGAG TTCAGGTGAT
    GAAGAAACTT TGGCCTTGCT CAAACGCCAG GGCTTGTTGC AGCAACCCGA GCAAGCTCCC
    TTCACATCAA GGATGGGGCT CCTGCTGGTC TTCCCCCTTA TTCAGTCCCA GAGTAGAACA
    GACCCCTCTC TCTGTAACAT CACTGCTGAG GTGCTATTGA ACTGCCTTCG CGACTGCCAG
    CCTTTGAGCC TGACCAAGGA GCCTGCTGAC TGTCTCAATG GAATTGAAAC TTTGCTGTGC
    TCCTGGCTAG AGGAGACTTC TGACACAGGC CAACACATCC CACATAAGCA AAAAGAAAAT
    GCTGCTGCTG CCCTCGTGGC TTTGGCATGT GCCAGGGGAT CATTGAAAAC TTTTGTTCAT
    ACAGTCCATC TATTACAGAA ACAGACTGAT GTAGGGTCCC TGCCTGTAGC GGATGTGCTG
    TATAGGCTGT TGCTTTTGGA AGGGGGGCCT GGATCCCCCT CCTGTTTGCT TGGTGGCAAA
    CATATAGTAT CCTGGGGGTA TGAAGACATG CTGCCTGCAC CAGATAGCAA CACTGGCTCC
    AGTTCTGAAA ATAAAGATGC TGACTTGGGA CGCTGTCTCA CGGCAGATGG TCTTTACCTG
    TATACTACTA ACTCAGTTGG AAGAGGAGTA AGCAAATTGG GGTCTGGATT ACATGGTACT
    CTCAGAGGTT TTGTGTACTG CCGGAACGAG GAACTGGAAC CAGGATGGCT TGCTTTTGGC
    AGTGGCAGTC TTCTCCACCG GCCTGTATCT TTTGACAATA AACCTCACTC CCTTTTCCAG
    GTCATTGACC AGAATACCCT TCAGGTGTGC CAGGTGGTGC CAATGCCAGC CAATCACCTC
    CCTGTTGGCA GCACTATGAG CACTGTGCAC CTTTCTTCAG ACGGCACTTA CTTCTATTGG
    ATCTGGTCTC CTGCCAGCCT GAATGAGAAA ACACCTAAGG GACATTCTGT CTTCATGGAC
    ACTTTTGAAC TTGTGGTGGA AAATGGTGTA TTCGTAGCCA ACCCGCTTCA GGAACGCACA
    ATTCTAATGA GAAAAGAGGG CGAGTCTGCA AAAAGCATTA ATGAGATGCT CCTGAGCAGA
    CTGTCCCGTT ACCGAGCCTC GCCGTCCGCA ACACTGGCTG CCCTCACCGG CTCCACCATC
    TCCAACACCC TGAAAGAGGA CCAAGCAGCA AATACTAGCT GTGGACTTCC TCTAAAAATG
    CTTCGGAAGA CACCCATTTA TACCTGTGGT ACATACTTGG TGATGCTGGT CCCACCTCCA
    GGGGGATCAG GCAGCTCTGC CACCCGCTCT CTTTTTGGTG GAACAAGTGG TTTGTCGTCT
    TTAAAAATCC TGGCCTCTAG CTTGGTGTAT AATATTTCGG ATGGTCAATT CACAAGTCGT
    GCCGACCTCA TAGATGCTGC TGGAAGCTCC TTGGGGCGTG GTGCTCTTGT ACCAGGATTG
    GGTGCCTGTT ATGATACAGT GAACAACATG CTGTGGACAT GCTCAAATGA CTATATCGAT
    CAGTGGTGTA ATCCTGGGAA TCAGGCCTTC CATTACGTCT GCCAGAGACT TGGAGTCAGC
    CATATCATCA CTGAGCCCAA AGAAGAGGCT ATAACCACGA ATGAGGTTAT AAACCAATTA
    TTGCACCACG TTGGTGCGAT GTGCATACAC CAACTCAATC TTCTTGCTAC CAACCCCAAT
    CTTCCAATCA CAAGTGTCTT GGGCAAACAG CATCCCATTG AAGCACACCA TCTGAGCAGT
    ATTTGTGACA TTATGGAGAA GGCCATGGTT AATGGAGACA CCTGTATTAT ACGCTGCATT
    CTTGTTGTCT TTCAGGTGGT GTTTAAATTT TTCTTCAGCC CACAAACTGA AAGGAATCGA
    GACATCATTC GACGGTCAGG ATTGCTTCTT TGGCAGTTGT TGATGGCACC CAAAGATCAA
    ATTTGCCCTG AAATTCAGAA GGAGGTCTGC CTTGCCATCA GCTCTGGTTT AAATATCTTG
    TACCCAGGTG AAACTGAAAT CAATAACTTA CTTAAGCTGG TCTTAACAGA AGGAGAGAGA
    AACAGTGGCC TCTCCCAGCT GCGGGATGTG ATCCTCACTA ATTTAGCTGA ACAACTTCAA
    AACAACCGAT TCGGCAGCGA GGAGGAGGAT CATTACAGAC TAAATGATGA ACTTTTACAC
    TACATTCTGA AGATTGTTGT ACGAGAATCC TGTATCTTAA TCACCAAGTG CCAAACTGTC
    TCTAAAGATG ATTTTCAAAA ACTCCTTTCA ACTGTGCCTG CCGCATCCTC CTGCCTGCGC
    TATCTGATGG CAGTTCAGAA CCACCTTCTC AGTAACACTA TTTTGATTAA GCCTGATGAG
    AATGATGACA GTGACAACTC CTTGCAGGGA GAGACATTGA AGGAGTTAAA GGTCAGTATT
    TTGGCTCTTG CTACCCAAAT CCTGACTGGA TGTGATGAAG TGTTGGAAAT GCTACAGCAG
    GTCACAACTG CCCTCATAAA TAGTGACATA CCCGACCGGG AGCAGAGGTT AAAAGGCTTG
    GAACAAGTTA CTAAGGCTAC TATGCTTGGT CACCTTCTCC CAGTGTTACT CACTTCCCTG
    ATGCATCCAA ATTTACAAAC TTTGACCATG GCCGATGCCC TGATGCCTCA GCTAGTGCAG
    CTGGTACTCT ATACCAGTCA GACGGCTTTG CTGCTTAAAA CCCAGTGTCC TGTTTTTGCT
    GAGGTGGGCT GTTCCCCATG TGGTGCATCA GACCAGAAGG GCAGGCTTTT GCCGGATGAG
    AGAATGCTGG AAGAGAAGGA GGAGCCAGGG TTTCTCACTG GTTTAAAGAT TCCTGCTCCA
    TGGGCTGCTG GGAAGACTGT GGAAACAGTC CACCCTGTCA GAGACAACTA TAAATTTAAA
    GAAACTGTCC ACATCCCCGG AGCTCGCTGC CTGTACCTTA GATTTGATAG CAGATGCTCT
    TCGCAATATG ACTACGACAA ATTGGTGATA TACGCGGGGC CTAACACAAA CAGTAGGAAG
    GTTGCTGAGT ATGGAGGCAA TACACTGGGA TATGGCAGCC GTAGTGTCTT AGGAACTGGT
    TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA GATACAGTCA CCTTCTCCTT TGAAATGAGA
    AGTGGCCGTG AACACAACAC TCCCGATAAG GCTATGTGGG GCTTTGCTTG CACAGTTCGC
    GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA GGCTTACCCT TTCTGGTAGA CCTGGCTTTA
    GGTCTATCTG TGTTAGCTTG TTCCATGTTA AGAATCCTAT ACAACGGACC AGAAATTACC
    AAAGAAGAAG AAACCTGTCA GGAGCTGTTG CGGTCCAAAC TTTTACAAAG GTGCCAGTGG
    CAGGTAGAGG CCAACGGTGT GATCTCCCCT GCCCTTACTC CAAGCCCATC TCCACTGCCT
    CTGACCATCG AGGAAGACAG AGAGTTCACT TACCCGTCTG ATGTCCTCGT GCCTCCTGTT
    GGAAACTACT TTGACCTGCC CCGGATCAGG CTGCCTCCAG GAATCATGAT AAAGCTCAGG
    GAAATTTCTG GGCGTGCTAG ACCACAGTTT AGACCAAGTA TAAAAGAAGT AATTCAGCCA
    GATGTGATGG AGGAAATGGT GGTATCATGT GTTATTAAGC ACTTGAACTT AGTTGATGCA
    CTGCAGTCCC TAATAAATTT CCAGTATCAA GAAGAACATG CTGAGGAATA TGATTTACTG
    TGTAAAATCA TGGGAGAGAC ATTTAAGAAA CTCAATGCCA TGGAGAGACA GCTGCAGAGT
    GTTGCAGAAT TGGAACAGAA ATGGCAGAGT GAAGTTGATG ATGCCATGCA GGGGAAACTG
    GAGAACAACA TGCCTTTCTT CTATGATTAC CATTTCAATG AGAACAAAAT GAAAGAACTA
    GAACTTTTGT GTTCAATGAA GGAAGTCTCC TTTGATGGAA ATGATCTTGA AAACATGGTG
    CTATCTCTGA GGGAGAAGTT CCTACAAGAA GTGAATTCTC TTATTCAGAA ACCCTCGCAC
    CCACTGGCTA AAACGAAGAC GTTAGTGAAG AGTCTAATGA ACCGAGCTGA GCTGTTGCTG
    CACGTCACCA TTGCAGCCCA GTCTGGCCTC ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA
    ACACCGGCTT GTAAATCAGC CTCTGAAACA AAGGTGATCT CTCATGCTGT GCGTCAGCCT
    GTTTTCCTTC GCAGCATGTC GGCTCCCTCT GACCTGGAAA TGATCGGTAA TGAAGATTTG
    GAATTTACTA GAGCAAATCA GAGGCGGCGC CACGTGACCA GCCACCGCAG CAGCTCCTTC
    ACACTCCTGC AGTCATTGGC CATAGAGGAC AGCAGGGACA AGCCCACCTA CAGCGTCCTG
    CTGGGGCAGC TGTTTGCTTT CATCGGCACC AACCCTGACC AAGCTGTGTC CAGCAGCAGC
    TTTCTTCTGG CCGCACAGAC GAGATGGCGG CGGGGAAACA CCCGCAAGCA GGCGTTGGTG
    CACATGCGGG AGCTGCTGAC GGCTGCCGTG CGAGTCGGGG GAGTGACACA CCTTGTGGGT
    CCAGTGACAA TGGTCCTGCA GGGAGGACCC AGAATTGAAG AACTGACCTG TGGTGGGATG
    GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG ACCATGACCT CTGTGGTGTC TTTGTGCGCA
    CGTTACCCTA TCGCATGTGC AAATAGCATC GGACTCTTGT GCACTATACC TTATACCAGG
    AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC CTTGTCCAGC TCATGGATCG ACTATGCAGC
    TTGAGCAGTC AGACTGAGTC CAGCTCCAGT GAGAAACAAA CCAAGAAGCA GAAGGTGGCC
    ACCATGGCCT GGGCAGCCTT CCAGGTGCTT GCCAACCGCT GCGTTGAATG GGAAAAAGAA
    GAAGGCGGCT CCACAGAGGC TGTGCACTCA GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC
    ACGAATCACC TTGCCCGAGC GACCGAGTGC TGTGGCAACC AGGCTGCCGG GAACGATGCC
    CTCCAGGACG TCCTGAGTCT CCTCAATGAT CTCTCGAGGA GTCATATAGG TAAAGCCATC
    CTGAGCCAGC CAGCTTGTGT GTCCAAACTC CTCTCCCTGC TACTTGACCA ACGCCCATCC
    CCAAAGCTGG TTCTTATCAT TCTTCAGCTG TGCCGGGCGG CACTCCCGCT GATGAGCGTA
    GAAGACTGTG GGAACGTGGA GCTCCCACCC TGGAGCTACT CCGTCCCCTC CCTAAACAGC
    GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC AAGATCGCCT CGCTGCTCTT GGCAAAGCTG
    GCAGACTACG TGGTCCCAGG ATGTCAGACA GTCCTTTCTC CAACCGCTTC CGAACCTGAC
    ACCACATTGA CAAAAACCAG CCCCAAGAAT TCCTTAAAAG GCGATAAAGA TCCTGGAGAA
    GAGAGTGAAG CAGTGGATGG CAAGCTCTCT ATCTTCATCC ACAAGCGGGA AGACCAGTCG
    TCCCATGAAG TTCTGCAGCC ATTACTAAGT AGCTCAGAAG GACGGCCCTT CCGACTTGGT
    ACTGGTGCCA ACATGGAGAA AGTTGTGAAA ATGGACCGAG ACATGACGAA GGGTGGCTGC
    TGTGAGGTGA TTACGGAAGA GGCGGCAGCT GCTCTGCGAA AAGCGACCAA GTGGGCACAG
    TCAGGCCTCA TTGTCAGTGT TGGGCCACCC GTGGAGTCCC TCAACCCAGA GGCCGTGAGC
    GGGCTGTCCA CAGGGGACAA GAAGAAGACT GCCCAAACCT CCATTTGCCG AGAGAGAAAC
    TCTGAACTCG CCAGGACCGA CCCCGTCCGA CCTTTCATCA GTGGGCACGT GGCAAACAGC
    ATGGCTGCTG AGGTGATCGC CTTGCTTCAT AGCTTGCTGA TGGCACCTGA GTCAAATGCT
    GCTCAGATAT GGACCACGAC AGCAGAGAAG GTTCTGTCTC GTGCACTGAT GTACATTCCA
    CAGTTGGGGA AATATGCAGA AAGCATTCTG GAAAATGGCA GCAGCAGTGG CAGAAAGCTT
    GCCAAACTTC AGAGAATTGC CCGCCAGGCT GTGGCTGCAC TCTGTGCACT TGGAGGCTTC
    AAAGAGACAA TCAAGATCGG GTCTGAGGTC CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC
    ATCGGAGTGG TGGCTTCTAT CAACGAACAG GAAGGTATAG CTACGGTCAG ATTCCCACCC
    ATAGACTGTA GAAAGACTTC GCAAGCATCA GACACACTGA CTATTCCATT GTCTAGACTT
    TGTGTTCCAA GATCAGAGGC ATTGCCTCTT CATAAACTGT CCATTACTGA GAAGGTAGTA
    CAGGCAGTCC AGTCCATGTT GCTTCCTCAG GAGGGAAGTC TCTCTATTCA TACCTCACTT
    CCTGCAGCAG GAGATGGGTC AGCTCCTGTG ATGGCAGTCG TTCGGCTCCT GGCTGAAATA
    AGAACAAGAG CATGCCTGGT CATGGCCCAG CTTTTAGAAG ACAGCTTATT TTGTGAAGAA
    TTCATACAGC AGTGTCCAGC AGCTGTTGAA GTTCTTAACC TAGTAGCCCA GGAATGCAGT
    GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG CAGTGTGAAC GGCTGAGGAT GCTCTACCGG
    GACTGTGCTC GGCCCCCGCC GCCTCCGCTG CAGGCTGACC GGAGACAGCC CAAAGAGATT
    ACGTGGAGCC CCTCACGAGT GTTTCCACCT GTCCGAGCCT GCATGTTCTC CTCGCACCTC
    ACTTCTGTCA CCTTCCTAGC AGACCCCAGT GCAGGGGGCG GTCTGCCCAG GGGCACATTT
    ATCTACGCCA CCTCACCACT GCCCGTTCAG GCCCCGTCTT TCTACTGGGA AATTGAAATT
    GTTTCCTATG GAGATACAGA TGATGATACT GGACCAATAG TTTCCTTTGG CTTTGCTACA
    GAAGCAGAGA AAAGAGATGG GGCATGGACT AATCCAGTGG GCACCTGTCT TTTCCATAAC
    AATGGCCGAG CTGTGCATTA CAATGGCTCC AGTTTATTAC AGTGGAAGAG CGTTCGATTA
    GACGTAACTC TCTCTCCTGG TGACGTGGCA GGGATTGGCT GGGAGAGAAC CGAGGGGACG
    CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC CGTGTGTACT TCACATACTG TGGGCAGCGC
    CTCTCACCGT ATCTCGAAGA TGTCTCGGGT GGGATGTGGC CGGTGGTTCA TATTCAGAAA
    AAGAACACCA AGACTCGCGC TAATTTTGGC TCCCGCCCAT TTGCCTACGC TGAAGGGCAA
    GCCCACCGCA ATGCTGCCGA CCTGTGCACT GACCTGGCCG AGGAAATTAG CGCTAACTTC
    GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC AGTGATAACG ATGCTGGCAC CAGTATTGCG
    TCAGACCCGG GCACTCATGG GCCACCATGC CGAATTGCTG CCGTAGCCAC TGCTCAGCAA
    CAATATGATA GTGATACTTC CTGTCATTAT AAAGTGGAGC TCAGCTATGA GAATTTCATC
    ACCTCTGGGC CAGACCCTCA CCCACCCCCC ATTGCAGATG ACGAAAGTGA TGATGATGAT
    GACGATGACA TCCCACAGGA GGATCACTAT GCCCTGCTGG TGAAAGCCTG GGAGACCAAG
    GTTTTTCCTA CCATCCGAAG ACGGTTCCGC AACGAAGCAG AGAGGAAATC AGGACTGGAC
    CAGATAAAGG GTGCTTTACA ACTAGGCATG GTGGATATTG CCCGGCAGAC AGTTGAATTC
    CTCTACGAGG AGAATGGTGG CATCCCAAGA GACCTTTATC TTCCCACCAT TGAAGACATT
    AAAGATGAAG CGAACAAGTT CACAATTGAT AAAGTTCGAA AAGGTCTCAC GGTAGTAACA
    CGCTCTCCAG ACAGCAATAA CGTAGCCAGC AGCACTGTTG GAACTGCCCT GCCAAAATTT
    GCTATCCGAG GGATGCTGAA AACCTTTGGG CTTCATGGAG TTGTCTTAGA TGTTGATTCA
    GTTAATGAAC TAGTTCAGGT AGAAACGTAC CTCCGAAGTG AAGGTGTGCT GGTCCGATAC
    TGGTACCCTA TTGATATGTT GGAAAGGCCC CCCGCAGGCT ACAGAAGGAC TGCCACCAAT
    GGGCTCGTCA CACTGGACAA TACCAACCTT CAAATTCACA GGGAGCTGCT GCGCTGCGAG
    GCCGCGCTGG CCAGGCTGTA CTGCCGCATG GCCCTGCTCA ACATCTTCGC CCCCAAGCTG
    CCTCACCTGT TTACCCGCCT CTTCCACATC CCCGCCATCC GGGACATTAC CCTGGAGCAC
    CTGCAGCTGC TGTCCAATCA GCTCCTCGCT CCTCCCCTCC CAGATGGCAC CATCAGCTCC
    AGCTCCATCC TCCTGGCGCA GTCCCTGCAG CACTGTGTCC ATTCCCAGAA CTGTTCGGCC
    ACAGACCTCT TCTACCAGGG CAGCTCCCAG ACGGTGCGAG AGTGGCTCAA CGTGGCCATC
    ACGCGGACCC TGCACCAGGG CGAGGAAAGC CTTCTGGAGC TGACCAAACA GATCTGCTCT
    TTCCTGCAGA CAGCACCAGA ACAGTTCCCC TCCGAAGAGT TTCCAATTTC CGAGTCCAAA
    GTCAACATGG ACGTCAATTT CCCCGGGGCA GCTTTCGTCG TCGTATCTTG CAAAGAAAGT
    CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG TACAAGGCAC CATGGGCGCG TGTGCTGGTG
    TACGGGCTAG GCCACAAAGT GAAGCGAAAT GGCCAGCTGA ACCTCATTGA GGCCGTCTGT
    TACCCGCGGG ATGCGTCTCC GGCCAACACA GGGCTCACAC CACCTCCTAC CACCAACCAG
    TACCCTTCTG TGATCCTCTC CACAGACAAA GTCCACATCA AACTGGGGGT ATCTCCCCCA
    CCCGGAGCCG TGCTGGTGTT ACACTCCCTG CCTCTCGAGT TCCCCCTGGC GATGGCCTTC
    ACCGAGCAGC TACTGTCCTG GAAATCAGAG GATGGCGAAG GTAGAGCTGA AGATGAGCCT
    GACACCATCC CGACGTCCGT CCTCCTGCAA GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG
    ACCACAGACA TGGCGGCCTG CGTGAAGGAG CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG
    CGCACGGTGC ACAGCCTAGA GCAGAGGAAG CACCCAGCCG GCCTGTCCTC ATCCATCGCC
    CTCCAGCTGA ACCCCTGCCT GGCCATGCTG ATGGCCCTGC AGTCGGAGCT TCACAAGCTG
    TACGACGAGG AGACGCAGAG CTGGGTCTCG GGCAGTGCAT GCGGAGGCTC AGGAGCCACG
    GCGGCTGCTG ACCAGGGCCG GTTCTCGACG TATTTCCACG CACTCATGGA GGGCTGCCTG
    GCGGTGGCCG AGGTGACCCT GCCCACCAAC ATGAGTGTCA CCGCCAGCGG GGTGACGTCA
    GCAACTGCCC CAAACCTCAG CGACTCGTCC TCCTCCTCAT CGTCCTCCCC AGGACAGACT
    CCACAGAGCC CCAGCCTCCT GTCCAAGAGG AAGAAAGTCA AGATGAAGCG GGAAAAGGCC
    TCCTCCTCTG GGAAGCGCCA GTCCTCACGC TCTGTGGACT CGGACCCCGC CGTGCTGAGC
    ATCGGGGGCA GCAAGCCCGA GGACATGCTG TGGTTCCATC GTGCTCTGAC GTTGCTCATC
    ATCCTCCGCC ACCTCACCAG GAAGGACCCA CAGGGGCTAG GCGTGACAAG CGACGCCATC
    GCCGACGCCT GCCAGGCCCT GGTGGGCCCC ACCGCCCACA GCCGCTTGCT GGTGATCTCT
    GGGATCCCCA CCCACCTGGA CGAGGGCATC GTCAGGGGTG CCATCCGCAA GGCCTGCAAT
    GCCCATGGCG GGGTGTTCAA GGATGAGATC TACATCCCCC TGCAGGACGA AGACCCCAAG
    AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT GGGAAAGCCG AGCCGGAAAA GACCCTCGGC
    TTCCCCAGCA CGGACAGCTT GGAGGTCAGC ACATCCAGCA GCCTGACCCC CGCTATGAGC
    GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT TCCATCTGCA GCTCCCAGGG CATCTCGCAG
    ACAGTCAGTG ACCTCTCTGC GGATCCGTTG CCTGCAGGCC TGGAGCTGCC CATCCCTGCA
    GGCTTGTTGG AGCCCCATGT GGTGTCCAGC CAGGAGAGCC TGGACATTTC CCTGTGTAGC
    ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG GGCGAGCCAC TGGACAATGC AGAAACGGCC
    TCAGTGTCAG ACATGGGCTC CATGTACACA GTCACGTCCC TGGACAACCA GCCCCTTGCA
    GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG GAGATAAGGT CCCGAGCCAA AATTGAGAAA
    ATTCGAGCAA GTTTATTTAA CAACAATGAC TTGATAGGTT TGTCAAGCTT GGATGGTGAG
    GATGAATTGA TGGAAATGTC AACGGAAGAG ATCCTCACGG TGTCTGTAGT GAATCAGAGT
    CTATTCGATA CGCAGGGGAG CCCAGGGTTA GAAGATTATT TCAATGATAA GTCAATTAAA
    GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG GTGCTGACGG AGATATTTAA GAGCTGTGCC
    CACTCGGAGC AGATGCTGAG CCTGACGCCA GCAAAGCCCA TCAAAGTGTC GGACATTTAT
    CTTAGCAAAG AGCAGATCAA CTCCCAGACA CCGGGCAACC TCCTCCACCT TTTCTTCACC
    AATGTCCGGC CCCCCAAAAA GGTGCTAGAG GACCAGCTCA CCCAGATCCT GAGGAAGTAC
    GGTGTGCCGA AGCCCAAGTT TGACAAGAGC AAGTACAGCA AGGCGGGGAA GGAGCAGCAC
    CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC ATCACCAAGC CGCCCACCAA GGACAAGGCA
    GTGCTCAACA GCGTCAGCAG GACTGCCTTG AGTGAGAAGA AGCCCACCGT GAAGCCCAAG
    TCACCTGAAA AGAGCAAGCC AGATGAAAAG GACCCTGAAA AGTCACCCAC CAAGAAACAA
    GACGTCCCAG AGGAGAAGTA CCTGACCTTA GAAGGATTTC ACAAGTTTGT GATCGACCGA
    GCCAAGCAAG ACATCCGCAG CGTCTGGAGA GCGATTTTAT CCTGTGGCTA CGATCTTCAT
    TTTGAGAGGT GTGCCTGCAT CGACGTCCGG CATGCGCACA AGGCCTCCAG GAAGTGGACC
    CTGGAGATGG ATGTGGCGCT CGTGCAGTAC ATCAACCGGC TGTGCCGCCA CCTGGCCATC
    ACGCCCGCGC GTCTGCATCC CCACGAGGTG TATCTGGACC CTGCAGACGC TGCTGACCCC
    AGAGTGGCCT GTCTCTTGAA CGTGCCCATC GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG
    CAGTCCCTCA ACACCACGCT CGAGACCTTC TTCCTGCCGC TGGTTGAGCT GCGCCAGACT
    CCCATGTACA CGCACAGCAT CGCCGCCCTG CTCAAGGAGG CCAAAGGGCT GATCTTTTAT
    GACACAAAGG TGACCGTCAT GAATCGGGTG CTAAATGCCA CGGTGCAGAG GACGGCTGAC
    CATGCAGCAC CAGAAATCAC TCTGGACCCG CTGGAGATCG TGGGAGGGGA GATCAGAGCC
    TCTGAGAACT CCTACTTCTG CCAGGCAGCC AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG
    CTTTGCGTCA AACTGGCGAG TGGCGGCGAC CCTACCTATG CCTTCAACAT CCGCTTCACG
    GGAGAGGAGG TCCATGGCAC CAGTGGCTCC TTCCGCCACT TCCTGTGGCA GGTGTGCAGA
    GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC CTGCTGTGCC CCAGCTCCGC CGTCAACAAG
    AACAAGGGCA AGTACATCCT GACCCCCAGC CCCATCACCT ATGGGGAGGA GCAGCTGCTG
    CACTTCCTGG GCCAGTTGCT AGGGATCGCC ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC
    CTCCTGCCCT CCTTCTGGAA GACACTGGTG GGCGAGCCCC TGGACCCTGA CCAAGACCTG
    CAGGAAGCAG ACGTACTCAC CTACAATTAC GTCAAGAAAT TTGAGAGTAT CAACGACGAG
    ACGGAGTTGG AGGCCCTGTG TGCTGAGATC GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC
    GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC ACCTACCTGA CCATGACCGG CGAGGAGGTG
    GAGCTGTGCA GCCGTGGCCG GCACATCCCC GTGGCGTGGG AGAACAAGGA CATCTATGCG
    GCCGCCATCC GGAGCCTGCG GCTGCGTGAG CTACAGAACG TGGAGTGTGT GACGGCCGTG
    CGCGCGGGCC TTGGGGCCAT CATCCCCCTG CAGCTGCTCA CCATGCTCAG CCCACTGGAG
    ATGGAGCTGC GCACCTGCGG CCTCCCGTAC ATCAACCTCG AGTTCCTGAA GGCCCACACC
    ATGTACCAGG TGGGGCTGAT GGAGACCGAC CAGCACATCG AGTTCTTCTG GGGGGCACTG
    GAGATGTTTG CCCAGGAGGA GCTGTGCAAG TTCATCAAGT TTGCCTGCAA CCAGGAGCGC
    ATCCCGTTCA CCTGCCCCTG CAAAGACGGG GGTCCTGACA CGGCCCATGT GCCCCCATAC
    CCCATGAAGA TTGCCCCCCC AGATGGCACA GCAGGTTCCC CAGATTCTCG CTACATCCGA
    GTGGAGACCT GTATGTTCAT GATCAAGCTT CCCCAGTACT CCTCGCTGGA AATCATGCTG
    GAGAAACTAC GTTGTGCCAT TCACTACCGC GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52729
    Clone ID Related Accession (Same CDS sequence) XM_010814057.2 , XM_015467161.1
    Accession Version XM_010814057.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGCAGGACT
    GCCTTGAGTG AGAAGAAGCC CACCGTGAAG CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT
    GAAAAGGACC CTGAAAAGTC ACCCACCAAG AAACAAGACG TCCCAGAGGA GAAGTACCTG
    ACCTTAGAAG GATTTCACAA GTTTGTGATC GACCGAGCCA AGCAAGACAT CCGCAGCGTC
    TGGAGAGCGA TTTTATCCTG TGGCTACGAT CTTCATTTTG AGAGGTGTGC CTGCATCGAC
    GTCCGGCATG CGCACAAGGC CTCCAGGAAG TGGACCCTGG AGATGGATGT GGCGCTCGTG
    CAGTACATCA ACCGGCTGTG CCGCCACCTG GCCATCACGC CCGCGCGTCT GCATCCCCAC
    GAGGTGTATC TGGACCCTGC AGACGCTGCT GACCCCAGAG TGGCCTGTCT CTTGAACGTG
    CCCATCGAGA GCCTGCGCCT GCGCTTCGCT CTGCTGCAGT CCCTCAACAC CACGCTCGAG
    ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC CAGACTCCCA TGTACACGCA CAGCATCGCC
    GCCCTGCTCA AGGAGGCCAA AGGGCTGATC TTTTATGACA CAAAGGTGAC CGTCATGAAT
    CGGGTGCTAA ATGCCACGGT GCAGAGGACG GCTGACCATG CAGCACCAGA AATCACTCTG
    GACCCGCTGG AGATCGTGGG AGGGGAGATC AGAGCCTCTG AGAACTCCTA CTTCTGCCAG
    GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC TCCCAGCTTT GCGTCAAACT GGCGAGTGGC
    GGCGACCCTA CCTATGCCTT CAACATCCGC TTCACGGGAG AGGAGGTCCA TGGCACCAGT
    GGCTCCTTCC GCCACTTCCT GTGGCAGGTG TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC
    CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC AACAAGAACA AGGGCAAGTA CATCCTGACC
    CCCAGCCCCA TCACCTATGG GGAGGAGCAG CTGCTGCACT TCCTGGGCCA GTTGCTAGGG
    ATCGCCATCC GAGCAGATGT CCCCCTCCCC CTGGACCTCC TGCCCTCCTT CTGGAAGACA
    CTGGTGGGCG AGCCCCTGGA CCCTGACCAA GACCTGCAGG AAGCAGACGT ACTCACCTAC
    AATTACGTCA AGAAATTTGA GAGTATCAAC GACGAGACGG AGTTGGAGGC CCTGTGTGCT
    GAGATCGCCT CCCAGCACCT GGCCACTGAG AGCCCCGAGG GGCCCAACAA GCCCTGCTGC
    CGGTTCACCT ACCTGACCAT GACCGGCGAG GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC
    ATCCCCGTGG CGTGGGAGAA CAAGGACATC TATGCGGCCG CCATCCGGAG CCTGCGGCTG
    CGTGAGCTAC AGAACGTGGA GTGTGTGACG GCCGTGCGCG CGGGCCTTGG GGCCATCATC
    CCCCTGCAGC TGCTCACCAT GCTCAGCCCA CTGGAGATGG AGCTGCGCAC CTGCGGCCTC
    CCGTACATCA ACCTCGAGTT CCTGAAGGCC CACACCATGT ACCAGGTGGG GCTGATGGAG
    ACCGACCAGC ACATCGAGTT CTTCTGGGGG GCACTGGAGA TGTTTGCCCA GGAGGAGCTG
    TGCAAGTTCA TCAAGTTTGC CTGCAACCAG GAGCGCATCC CGTTCACCTG CCCCTGCAAA
    GACGGGGGTC CTGACACGGC CCATGTGCCC CCATACCCCA TGAAGATTGC CCCCCCAGAT
    GGCACAGCAG GTTCCCCAGA TTCTCGCTAC ATCCGAGTGG AGACCTGTAT GTTCATGATC
    AAGCTTCCCC AGTACTCCTC GCTGGAAATC ATGCTGGAGA AACTACGTTG TGCCATTCAC
    TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812359.1
    CDS1026..12752
    Translation

    Target ORF information:

    RefSeq Version XM_010814057.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGCAGGACT
    GCCTTGAGTG AGAAGAAGCC CACCGTGAAG CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT
    GAAAAGGACC CTGAAAAGTC ACCCACCAAG AAACAAGACG TCCCAGAGGA GAAGTACCTG
    ACCTTAGAAG GATTTCACAA GTTTGTGATC GACCGAGCCA AGCAAGACAT CCGCAGCGTC
    TGGAGAGCGA TTTTATCCTG TGGCTACGAT CTTCATTTTG AGAGGTGTGC CTGCATCGAC
    GTCCGGCATG CGCACAAGGC CTCCAGGAAG TGGACCCTGG AGATGGATGT GGCGCTCGTG
    CAGTACATCA ACCGGCTGTG CCGCCACCTG GCCATCACGC CCGCGCGTCT GCATCCCCAC
    GAGGTGTATC TGGACCCTGC AGACGCTGCT GACCCCAGAG TGGCCTGTCT CTTGAACGTG
    CCCATCGAGA GCCTGCGCCT GCGCTTCGCT CTGCTGCAGT CCCTCAACAC CACGCTCGAG
    ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC CAGACTCCCA TGTACACGCA CAGCATCGCC
    GCCCTGCTCA AGGAGGCCAA AGGGCTGATC TTTTATGACA CAAAGGTGAC CGTCATGAAT
    CGGGTGCTAA ATGCCACGGT GCAGAGGACG GCTGACCATG CAGCACCAGA AATCACTCTG
    GACCCGCTGG AGATCGTGGG AGGGGAGATC AGAGCCTCTG AGAACTCCTA CTTCTGCCAG
    GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC TCCCAGCTTT GCGTCAAACT GGCGAGTGGC
    GGCGACCCTA CCTATGCCTT CAACATCCGC TTCACGGGAG AGGAGGTCCA TGGCACCAGT
    GGCTCCTTCC GCCACTTCCT GTGGCAGGTG TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC
    CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC AACAAGAACA AGGGCAAGTA CATCCTGACC
    CCCAGCCCCA TCACCTATGG GGAGGAGCAG CTGCTGCACT TCCTGGGCCA GTTGCTAGGG
    ATCGCCATCC GAGCAGATGT CCCCCTCCCC CTGGACCTCC TGCCCTCCTT CTGGAAGACA
    CTGGTGGGCG AGCCCCTGGA CCCTGACCAA GACCTGCAGG AAGCAGACGT ACTCACCTAC
    AATTACGTCA AGAAATTTGA GAGTATCAAC GACGAGACGG AGTTGGAGGC CCTGTGTGCT
    GAGATCGCCT CCCAGCACCT GGCCACTGAG AGCCCCGAGG GGCCCAACAA GCCCTGCTGC
    CGGTTCACCT ACCTGACCAT GACCGGCGAG GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC
    ATCCCCGTGG CGTGGGAGAA CAAGGACATC TATGCGGCCG CCATCCGGAG CCTGCGGCTG
    CGTGAGCTAC AGAACGTGGA GTGTGTGACG GCCGTGCGCG CGGGCCTTGG GGCCATCATC
    CCCCTGCAGC TGCTCACCAT GCTCAGCCCA CTGGAGATGG AGCTGCGCAC CTGCGGCCTC
    CCGTACATCA ACCTCGAGTT CCTGAAGGCC CACACCATGT ACCAGGTGGG GCTGATGGAG
    ACCGACCAGC ACATCGAGTT CTTCTGGGGG GCACTGGAGA TGTTTGCCCA GGAGGAGCTG
    TGCAAGTTCA TCAAGTTTGC CTGCAACCAG GAGCGCATCC CGTTCACCTG CCCCTGCAAA
    GACGGGGGTC CTGACACGGC CCATGTGCCC CCATACCCCA TGAAGATTGC CCCCCCAGAT
    GGCACAGCAG GTTCCCCAGA TTCTCGCTAC ATCCGAGTGG AGACCTGTAT GTTCATGATC
    AAGCTTCCCC AGTACTCCTC GCTGGAAATC ATGCTGGAGA AACTACGTTG TGCCATTCAC
    TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52729
    Clone ID Related Accession (Same CDS sequence) XM_010814057.2 , XM_015467161.1
    Accession Version XM_015467161.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11727bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1741
    1801
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    1981
    2041
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    2641
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    10501
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    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGCAGGACT
    GCCTTGAGTG AGAAGAAGCC CACCGTGAAG CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT
    GAAAAGGACC CTGAAAAGTC ACCCACCAAG AAACAAGACG TCCCAGAGGA GAAGTACCTG
    ACCTTAGAAG GATTTCACAA GTTTGTGATC GACCGAGCCA AGCAAGACAT CCGCAGCGTC
    TGGAGAGCGA TTTTATCCTG TGGCTACGAT CTTCATTTTG AGAGGTGTGC CTGCATCGAC
    GTCCGGCATG CGCACAAGGC CTCCAGGAAG TGGACCCTGG AGATGGATGT GGCGCTCGTG
    CAGTACATCA ACCGGCTGTG CCGCCACCTG GCCATCACGC CCGCGCGTCT GCATCCCCAC
    GAGGTGTATC TGGACCCTGC AGACGCTGCT GACCCCAGAG TGGCCTGTCT CTTGAACGTG
    CCCATCGAGA GCCTGCGCCT GCGCTTCGCT CTGCTGCAGT CCCTCAACAC CACGCTCGAG
    ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC CAGACTCCCA TGTACACGCA CAGCATCGCC
    GCCCTGCTCA AGGAGGCCAA AGGGCTGATC TTTTATGACA CAAAGGTGAC CGTCATGAAT
    CGGGTGCTAA ATGCCACGGT GCAGAGGACG GCTGACCATG CAGCACCAGA AATCACTCTG
    GACCCGCTGG AGATCGTGGG AGGGGAGATC AGAGCCTCTG AGAACTCCTA CTTCTGCCAG
    GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC TCCCAGCTTT GCGTCAAACT GGCGAGTGGC
    GGCGACCCTA CCTATGCCTT CAACATCCGC TTCACGGGAG AGGAGGTCCA TGGCACCAGT
    GGCTCCTTCC GCCACTTCCT GTGGCAGGTG TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC
    CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC AACAAGAACA AGGGCAAGTA CATCCTGACC
    CCCAGCCCCA TCACCTATGG GGAGGAGCAG CTGCTGCACT TCCTGGGCCA GTTGCTAGGG
    ATCGCCATCC GAGCAGATGT CCCCCTCCCC CTGGACCTCC TGCCCTCCTT CTGGAAGACA
    CTGGTGGGCG AGCCCCTGGA CCCTGACCAA GACCTGCAGG AAGCAGACGT ACTCACCTAC
    AATTACGTCA AGAAATTTGA GAGTATCAAC GACGAGACGG AGTTGGAGGC CCTGTGTGCT
    GAGATCGCCT CCCAGCACCT GGCCACTGAG AGCCCCGAGG GGCCCAACAA GCCCTGCTGC
    CGGTTCACCT ACCTGACCAT GACCGGCGAG GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC
    ATCCCCGTGG CGTGGGAGAA CAAGGACATC TATGCGGCCG CCATCCGGAG CCTGCGGCTG
    CGTGAGCTAC AGAACGTGGA GTGTGTGACG GCCGTGCGCG CGGGCCTTGG GGCCATCATC
    CCCCTGCAGC TGCTCACCAT GCTCAGCCCA CTGGAGATGG AGCTGCGCAC CTGCGGCCTC
    CCGTACATCA ACCTCGAGTT CCTGAAGGCC CACACCATGT ACCAGGTGGG GCTGATGGAG
    ACCGACCAGC ACATCGAGTT CTTCTGGGGG GCACTGGAGA TGTTTGCCCA GGAGGAGCTG
    TGCAAGTTCA TCAAGTTTGC CTGCAACCAG GAGCGCATCC CGTTCACCTG CCCCTGCAAA
    GACGGGGGTC CTGACACGGC CCATGTGCCC CCATACCCCA TGAAGATTGC CCCCCCAGAT
    GGCACAGCAG GTTCCCCAGA TTCTCGCTAC ATCCGAGTGG AGACCTGTAT GTTCATGATC
    AAGCTTCCCC AGTACTCCTC GCTGGAAATC ATGCTGGAGA AACTACGTTG TGCCATTCAC
    TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322647.1
    CDS1026..12752
    Translation

    Target ORF information:

    RefSeq Version XM_015467161.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467161.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGCAGGACT
    GCCTTGAGTG AGAAGAAGCC CACCGTGAAG CCCAAGTCAC CTGAAAAGAG CAAGCCAGAT
    GAAAAGGACC CTGAAAAGTC ACCCACCAAG AAACAAGACG TCCCAGAGGA GAAGTACCTG
    ACCTTAGAAG GATTTCACAA GTTTGTGATC GACCGAGCCA AGCAAGACAT CCGCAGCGTC
    TGGAGAGCGA TTTTATCCTG TGGCTACGAT CTTCATTTTG AGAGGTGTGC CTGCATCGAC
    GTCCGGCATG CGCACAAGGC CTCCAGGAAG TGGACCCTGG AGATGGATGT GGCGCTCGTG
    CAGTACATCA ACCGGCTGTG CCGCCACCTG GCCATCACGC CCGCGCGTCT GCATCCCCAC
    GAGGTGTATC TGGACCCTGC AGACGCTGCT GACCCCAGAG TGGCCTGTCT CTTGAACGTG
    CCCATCGAGA GCCTGCGCCT GCGCTTCGCT CTGCTGCAGT CCCTCAACAC CACGCTCGAG
    ACCTTCTTCC TGCCGCTGGT TGAGCTGCGC CAGACTCCCA TGTACACGCA CAGCATCGCC
    GCCCTGCTCA AGGAGGCCAA AGGGCTGATC TTTTATGACA CAAAGGTGAC CGTCATGAAT
    CGGGTGCTAA ATGCCACGGT GCAGAGGACG GCTGACCATG CAGCACCAGA AATCACTCTG
    GACCCGCTGG AGATCGTGGG AGGGGAGATC AGAGCCTCTG AGAACTCCTA CTTCTGCCAG
    GCAGCCAGGC AGCTGGCCTC AGTGCCCTCC TCCCAGCTTT GCGTCAAACT GGCGAGTGGC
    GGCGACCCTA CCTATGCCTT CAACATCCGC TTCACGGGAG AGGAGGTCCA TGGCACCAGT
    GGCTCCTTCC GCCACTTCCT GTGGCAGGTG TGCAGAGAGC TGCAGAGCTC CTCGCTCTCC
    CTCCTCCTGC TGTGCCCCAG CTCCGCCGTC AACAAGAACA AGGGCAAGTA CATCCTGACC
    CCCAGCCCCA TCACCTATGG GGAGGAGCAG CTGCTGCACT TCCTGGGCCA GTTGCTAGGG
    ATCGCCATCC GAGCAGATGT CCCCCTCCCC CTGGACCTCC TGCCCTCCTT CTGGAAGACA
    CTGGTGGGCG AGCCCCTGGA CCCTGACCAA GACCTGCAGG AAGCAGACGT ACTCACCTAC
    AATTACGTCA AGAAATTTGA GAGTATCAAC GACGAGACGG AGTTGGAGGC CCTGTGTGCT
    GAGATCGCCT CCCAGCACCT GGCCACTGAG AGCCCCGAGG GGCCCAACAA GCCCTGCTGC
    CGGTTCACCT ACCTGACCAT GACCGGCGAG GAGGTGGAGC TGTGCAGCCG TGGCCGGCAC
    ATCCCCGTGG CGTGGGAGAA CAAGGACATC TATGCGGCCG CCATCCGGAG CCTGCGGCTG
    CGTGAGCTAC AGAACGTGGA GTGTGTGACG GCCGTGCGCG CGGGCCTTGG GGCCATCATC
    CCCCTGCAGC TGCTCACCAT GCTCAGCCCA CTGGAGATGG AGCTGCGCAC CTGCGGCCTC
    CCGTACATCA ACCTCGAGTT CCTGAAGGCC CACACCATGT ACCAGGTGGG GCTGATGGAG
    ACCGACCAGC ACATCGAGTT CTTCTGGGGG GCACTGGAGA TGTTTGCCCA GGAGGAGCTG
    TGCAAGTTCA TCAAGTTTGC CTGCAACCAG GAGCGCATCC CGTTCACCTG CCCCTGCAAA
    GACGGGGGTC CTGACACGGC CCATGTGCCC CCATACCCCA TGAAGATTGC CCCCCCAGAT
    GGCACAGCAG GTTCCCCAGA TTCTCGCTAC ATCCGAGTGG AGACCTGTAT GTTCATGATC
    AAGCTTCCCC AGTACTCCTC GCTGGAAATC ATGCTGGAGA AACTACGTTG TGCCATTCAC
    TACCGCGAAG ACCCTCTGAG TGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52730
    Clone ID Related Accession (Same CDS sequence) XM_010814058.2 , XM_015467163.1
    Accession Version XM_010814058.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814058.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1921
    1981
    2041
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    2641
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    9781
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    10501
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    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATCTAAC AGTATATTTT CTGTTTTGAA GTTGGTGATA
    TACGCGGGGC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGA AGTGGCCGTG AACACAACAC TCCCGATAAG
    GCTATGTGGG GCTTTGCTTG CACAGTTCGC GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA
    GGCTTACCCT TTCTGGTAGA CCTGGCTTTA GGTCTATCTG TGTTAGCTTG TTCCATGTTA
    AGAATCCTAT ACAACGGACC AGAAATTACC AAAGAAGAAG AAACCTGTCA GGAGCTGTTG
    CGGTCCAAAC TTTTACAAAG GTGCCAGTGG CAGGTAGAGG CCAACGGTGT GATCTCCCCT
    GCCCTTACTC CAAGCCCATC TCCACTGCCT CTGACCATCG AGGAAGACAG AGAGTTCACT
    TACCCGTCTG ATGTCCTCGT GCCTCCTGTT GGAAACTACT TTGACCTGCC CCGGATCAGG
    CTGCCTCCAG GAATCATGAT AAAGCTCAGG GAAATTTCTG GGCGTGCTAG ACCACAGTTT
    AGACCAAGTA TAAAAGAAGT AATTCAGCCA GATGTGATGG AGGAAATGGT GGTATCATGT
    GTTATTAAGC ACTTGAACTT AGTTGATGCA CTGCAGTCCC TAATAAATTT CCAGTATCAA
    GAAGAACATG CTGAGGAATA TGATTTACTG TGTAAAATCA TGGGAGAGAC ATTTAAGAAA
    CTCAATGCCA TGGAGAGACA GCTGCAGAGT GTTGCAGAAT TGGAACAGAA ATGGCAGAGT
    GAAGTTGATG ATGCCATGCA GGGGAAACTG GAGAACAACA TGCCTTTCTT CTATGATTAC
    CATTTCAATG AGAACAAAAT GAAAGAACTA GAACTTTTGT GTTCAATGAA GGAAGTCTCC
    TTTGATGGAA ATGATCTTGA AAACATGGTG CTATCTCTGA GGGAGAAGTT CCTACAAGAA
    GTGAATTCTC TTATTCAGAA ACCCTCGCAC CCACTGGCTA AAACGAAGAC GTTAGTGAAG
    AGTCTAATGA ACCGAGCTGA GCTGTTGCTG CACGTCACCA TTGCAGCCCA GTCTGGCCTC
    ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA ACACCGGCTT GTAAATCAGC CTCTGAAACA
    AAGGTGATCT CTCATGCTGT GCGTCAGCCT GTTTTCCTTC GCAGCATGTC GGCTCCCTCT
    GACCTGGAAA TGATCGGTAA TGAAGATTTG GAATTTACTA GAGCAAATCA GAGGCGGCGC
    CACGTGACCA GCCACCGCAG CAGCTCCTTC ACACTCCTGC AGTCATTGGC CATAGAGGAC
    AGCAGGGACA AGCCCACCTA CAGCGTCCTG CTGGGGCAGC TGTTTGCTTT CATCGGCACC
    AACCCTGACC AAGCTGTGTC CAGCAGCAGC TTTCTTCTGG CCGCACAGAC GAGATGGCGG
    CGGGGAAACA CCCGCAAGCA GGCGTTGGTG CACATGCGGG AGCTGCTGAC GGCTGCCGTG
    CGAGTCGGGG GAGTGACACA CCTTGTGGGT CCAGTGACAA TGGTCCTGCA GGGAGGACCC
    AGAATTGAAG AACTGACCTG TGGTGGGATG GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG
    ACCATGACCT CTGTGGTGTC TTTGTGCGCA CGTTACCCTA TCGCATGTGC AAATAGCATC
    GGACTCTTGT GCACTATACC TTATACCAGG AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC
    CTTGTCCAGC TCATGGATCG ACTATGCAGC TTGAGCAGTC AGACTGAGTC CAGCTCCAGT
    GAGAAACAAA CCAAGAAGCA GAAGGTGGCC ACCATGGCCT GGGCAGCCTT CCAGGTGCTT
    GCCAACCGCT GCGTTGAATG GGAAAAAGAA GAAGGCGGCT CCACAGAGGC TGTGCACTCA
    GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC ACGAATCACC TTGCCCGAGC GACCGAGTGC
    TGTGGCAACC AGGCTGCCGG GAACGATGCC CTCCAGGACG TCCTGAGTCT CCTCAATGAT
    CTCTCGAGGA GTCATATAGG TAAAGCCATC CTGAGCCAGC CAGCTTGTGT GTCCAAACTC
    CTCTCCCTGC TACTTGACCA ACGCCCATCC CCAAAGCTGG TTCTTATCAT TCTTCAGCTG
    TGCCGGGCGG CACTCCCGCT GATGAGCGTA GAAGACTGTG GGAACGTGGA GCTCCCACCC
    TGGAGCTACT CCGTCCCCTC CCTAAACAGC GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC
    AAGATCGCCT CGCTGCTCTT GGCAAAGCTG GCAGACTACG TGGTCCCAGG ATGTCAGACA
    GTCCTTTCTC CAACCGCTTC CGAACCTGAC ACCACATTGA CAAAAACCAG CCCCAAGAAT
    TCCTTAAAAG GCGATAAAGA TCCTGGAGAA GAGAGTGAAG CAGTGGATGG CAAGCTCTCT
    ATCTTCATCC ACAAGCGGGA AGACCAGTCG TCCCATGAAG TTCTGCAGCC ATTACTAAGT
    AGCTCAGAAG GACGGCCCTT CCGACTTGGT ACTGGTGCCA ACATGGAGAA AGTTGTGAAA
    ATGGACCGAG ACATGACGAA GGGTGGCTGC TGTGAGGTGA TTACGGAAGA GGCGGCAGCT
    GCTCTGCGAA AAGCGACCAA GTGGGCACAG TCAGGCCTCA TTGTCAGTGT TGGGCCACCC
    GTGGAGTCCC TCAACCCAGA GGCCGTGAGC GGGCTGTCCA CAGGGGACAA GAAGAAGACT
    GCCCAAACCT CCATTTGCCG AGAGAGAAAC TCTGAACTCG CCAGGACCGA CCCCGTCCGA
    CCTTTCATCA GTGGGCACGT GGCAAACAGC ATGGCTGCTG AGGTGATCGC CTTGCTTCAT
    AGCTTGCTGA TGGCACCTGA GTCAAATGCT GCTCAGATAT GGACCACGAC AGCAGAGAAG
    GTTCTGTCTC GTGCACTGAT GTACATTCCA CAGTTGGGGA AATATGCAGA AAGCATTCTG
    GAAAATGGCA GCAGCAGTGG CAGAAAGCTT GCCAAACTTC AGAGAATTGC CCGCCAGGCT
    GTGGCTGCAC TCTGTGCACT TGGAGGCTTC AAAGAGACAA TCAAGATCGG GTCTGAGGTC
    CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC ATCGGAGTGG TGGCTTCTAT CAACGAACAG
    GAAGGTATAG CTACGGTCAG ATTCCCACCC ATAGACTGTA GAAAGACTTC GCAAGCATCA
    GACACACTGA CTATTCCATT GTCTAGACTT TGTGTTCCAA GATCAGAGGC ATTGCCTCTT
    CATAAACTGT CCATTACTGA GAAGGTAGTA CAGGCAGTCC AGTCCATGTT GCTTCCTCAG
    GAGGGAAGTC TCTCTATTCA TACCTCACTT CCTGCAGCAG GAGATGGGTC AGCTCCTGTG
    ATGGCAGTCG TTCGGCTCCT GGCTGAAATA AGAACAAGAG CATGCCTGGT CATGGCCCAG
    CTTTTAGAAG ACAGCTTATT TTGTGAAGAA TTCATACAGC AGTGTCCAGC AGCTGTTGAA
    GTTCTTAACC TAGTAGCCCA GGAATGCAGT GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG
    CAGTGTGAAC GGCTGAGGAT GCTCTACCGG GACTGTGCTC GGCCCCCGCC GCCTCCGCTG
    CAGGCTGACC GGAGACAGCC CAAAGAGATT ACGTGGAGCC CCTCACGAGT GTTTCCACCT
    GTCCGAGCCT GCATGTTCTC CTCGCACCTC ACTTCTGTCA CCTTCCTAGC AGACCCCAGT
    GCAGGGGGCG GTCTGCCCAG GGGCACATTT ATCTACGCCA CCTCACCACT GCCCGTTCAG
    GCCCCGTCTT TCTACTGGGA AATTGAAATT GTTTCCTATG GAGATACAGA TGATGATACT
    GGACCAATAG TTTCCTTTGG CTTTGCTACA GAAGCAGAGA AAAGAGATGG GGCATGGACT
    AATCCAGTGG GCACCTGTCT TTTCCATAAC AATGGCCGAG CTGTGCATTA CAATGGCTCC
    AGTTTATTAC AGTGGAAGAG CGTTCGATTA GACGTAACTC TCTCTCCTGG TGACGTGGCA
    GGGATTGGCT GGGAGAGAAC CGAGGGGACG CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC
    CGTGTGTACT TCACATACTG TGGGCAGCGC CTCTCACCGT ATCTCGAAGA TGTCTCGGGT
    GGGATGTGGC CGGTGGTTCA TATTCAGAAA AAGAACACCA AGACTCGCGC TAATTTTGGC
    TCCCGCCCAT TTGCCTACGC TGAAGGGCAA GCCCACCGCA ATGCTGCCGA CCTGTGCACT
    GACCTGGCCG AGGAAATTAG CGCTAACTTC GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC
    AGTGATAACG ATGCTGGCAC CAGTATTGCG TCAGACCCGG GCACTCATGG GCCACCATGC
    CGAATTGCTG CCGTAGCCAC TGCTCAGCAA CAATATGATA GTGATACTTC CTGTCATTAT
    AAAGTGGAGC TCAGCTATGA GAATTTCATC ACCTCTGGGC CAGACCCTCA CCCACCCCCC
    ATTGCAGATG ACGAAAGTGA TGATGATGAT GACGATGACA TCCCACAGGA GGATCACTAT
    GCCCTGCTGG TGAAAGCCTG GGAGACCAAG GTTTTTCCTA CCATCCGAAG ACGGTTCCGC
    AACGAAGCAG AGAGGAAATC AGGACTGGAC CAGATAAAGG GTGCTTTACA ACTAGGCATG
    GTGGATATTG CCCGGCAGAC AGTTGAATTC CTCTACGAGG AGAATGGTGG CATCCCAAGA
    GACCTTTATC TTCCCACCAT TGAAGACATT AAAGATGAAG CGAACAAGTT CACAATTGAT
    AAAGTTCGAA AAGGTCTCAC GGTAGTAACA CGCTCTCCAG ACAGCAATAA CGTAGCCAGC
    AGCACTGTTG GAACTGCCCT GCCAAAATTT GCTATCCGAG GGATGCTGAA AACCTTTGGG
    CTTCATGGAG TTGTCTTAGA TGTTGATTCA GTTAATGAAC TAGTTCAGGT AGAAACGTAC
    CTCCGAAGTG AAGGTGTGCT GGTCCGATAC TGGTACCCTA TTGATATGTT GGAAAGGCCC
    CCCGCAGGCT ACAGAAGGAC TGCCACCAAT GGGCTCGTCA CACTGGACAA TACCAACCTT
    CAAATTCACA GGGAGCTGCT GCGCTGCGAG GCCGCGCTGG CCAGGCTGTA CTGCCGCATG
    GCCCTGCTCA ACATCTTCGC CCCCAAGCTG CCTCACCTGT TTACCCGCCT CTTCCACATC
    CCCGCCATCC GGGACATTAC CCTGGAGCAC CTGCAGCTGC TGTCCAATCA GCTCCTCGCT
    CCTCCCCTCC CAGATGGCAC CATCAGCTCC AGCTCCATCC TCCTGGCGCA GTCCCTGCAG
    CACTGTGTCC ATTCCCAGAA CTGTTCGGCC ACAGACCTCT TCTACCAGGG CAGCTCCCAG
    ACGGTGCGAG AGTGGCTCAA CGTGGCCATC ACGCGGACCC TGCACCAGGG CGAGGAAAGC
    CTTCTGGAGC TGACCAAACA GATCTGCTCT TTCCTGCAGA CAGCACCAGA ACAGTTCCCC
    TCCGAAGAGT TTCCAATTTC CGAGTCCAAA GTCAACATGG ACGTCAATTT CCCCGGGGCA
    GCTTTCGTCG TCGTATCTTG CAAAGAAAGT CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG
    TACAAGGCAC CATGGGCGCG TGTGCTGGTG TACGGGCTAG GCCACAAAGT GAAGCGAAAT
    GGCCAGCTGA ACCTCATTGA GGCCGTCTGT TACCCGCGGG ATGCGTCTCC GGCCAACACA
    GGGCTCACAC CACCTCCTAC CACCAACCAG TACCCTTCTG TGATCCTCTC CACAGACAAA
    GTCCACATCA AACTGGGGGT ATCTCCCCCA CCCGGAGCCG TGCTGGTGTT ACACTCCCTG
    CCTCTCGAGT TCCCCCTGGC GATGGCCTTC ACCGAGCAGC TACTGTCCTG GAAATCAGAG
    GATGGCGAAG GTAGAGCTGA AGATGAGCCT GACACCATCC CGACGTCCGT CCTCCTGCAA
    GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG ACCACAGACA TGGCGGCCTG CGTGAAGGAG
    CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG CGCACGGTGC ACAGCCTAGA GCAGAGGAAG
    CACCCAGCCG GCCTGTCCTC ATCCATCGCC CTCCAGCTGA ACCCCTGCCT GGCCATGCTG
    ATGGCCCTGC AGTCGGAGCT TCACAAGCTG TACGACGAGG AGACGCAGAG CTGGGTCTCG
    GGCAGTGCAT GCGGAGGCTC AGGAGCCACG GCGGCTGCTG ACCAGGGCCG GTTCTCGACG
    TATTTCCACG CACTCATGGA GGGCTGCCTG GCGGTGGCCG AGGTGACCCT GCCCACCAAC
    ATGAGTGTCA CCGCCAGCGG GGTGACGTCA GCAACTGCCC CAAACCTCAG CGACTCGTCC
    TCCTCCTCAT CGTCCTCCCC AGGACAGACT CCACAGAGCC CCAGCCTCCT GTCCAAGAGG
    AAGAAAGTCA AGATGAAGCG GGAAAAGGCC TCCTCCTCTG GGAAGCGCCA GTCCTCACGC
    TCTGTGGACT CGGACCCCGC CGTGCTGAGC ATCGGGGGCA GCAAGCCCGA GGACATGCTG
    TGGTTCCATC GTGCTCTGAC GTTGCTCATC ATCCTCCGCC ACCTCACCAG GAAGGACCCA
    CAGGGGCTAG GCGTGACAAG CGACGCCATC GCCGACGCCT GCCAGGCCCT GGTGGGCCCC
    ACCGCCCACA GCCGCTTGCT GGTGATCTCT GGGATCCCCA CCCACCTGGA CGAGGGCATC
    GTCAGGGGTG CCATCCGCAA GGCCTGCAAT GCCCATGGCG GGGTGTTCAA GGATGAGATC
    TACATCCCCC TGCAGGACGA AGACCCCAAG AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT
    GGGAAAGCCG AGCCGGAAAA GACCCTCGGC TTCCCCAGCA CGGACAGCTT GGAGGTCAGC
    ACATCCAGCA GCCTGACCCC CGCTATGAGC GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT
    TCCATCTGCA GCTCCCAGGG CATCTCGCAG ACAGTCAGTG ACCTCTCTGC GGATCCGTTG
    CCTGCAGGCC TGGAGCTGCC CATCCCTGCA GGCTTGTTGG AGCCCCATGT GGTGTCCAGC
    CAGGAGAGCC TGGACATTTC CCTGTGTAGC ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG
    GGCGAGCCAC TGGACAATGC AGAAACGGCC TCAGTGTCAG ACATGGGCTC CATGTACACA
    GTCACGTCCC TGGACAACCA GCCCCTTGCA GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG
    GAGATAAGGT CCCGAGCCAA AATTGAGAAA ATTCGAGCAA GTTTATTTAA CAACAATGAC
    TTGATAGGTT TGTCAAGCTT GGATGGTGAG GATGAATTGA TGGAAATGTC AACGGAAGAG
    ATCCTCACGG TGTCTGTAGT GAATCAGAGT CTATTCGATA CGCAGGGGAG CCCAGGGTTA
    GAAGATTATT TCAATGATAA GTCAATTAAA GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG
    GTGCTGACGG AGATATTTAA GAGCTGTGCC CACTCGGAGC AGATGCTGAG CCTGACGCCA
    GCAAAGCCCA TCAAAGTGTC GGACATTTAT CTTAGCAAAG AGCAGATCAA CTCCCAGACA
    CCGGGCAACC TCCTCCACCT TTTCTTCACC AATGTCCGGC CCCCCAAAAA GGTGCTAGAG
    GACCAGCTCA CCCAGATCCT GAGGAAGTAC GGTGTGCCGA AGCCCAAGTT TGACAAGAGC
    AAGTACAGCA AGGCGGGGAA GGAGCAGCAC CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC
    ATCACCAAGC CGCCCACCAA GGACAAGGCA GTGCTCAACA GCGTCAGCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812360.1
    CDS1026..12746
    Translation

    Target ORF information:

    RefSeq Version XM_010814058.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814058.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATCTAAC AGTATATTTT CTGTTTTGAA GTTGGTGATA
    TACGCGGGGC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGA AGTGGCCGTG AACACAACAC TCCCGATAAG
    GCTATGTGGG GCTTTGCTTG CACAGTTCGC GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA
    GGCTTACCCT TTCTGGTAGA CCTGGCTTTA GGTCTATCTG TGTTAGCTTG TTCCATGTTA
    AGAATCCTAT ACAACGGACC AGAAATTACC AAAGAAGAAG AAACCTGTCA GGAGCTGTTG
    CGGTCCAAAC TTTTACAAAG GTGCCAGTGG CAGGTAGAGG CCAACGGTGT GATCTCCCCT
    GCCCTTACTC CAAGCCCATC TCCACTGCCT CTGACCATCG AGGAAGACAG AGAGTTCACT
    TACCCGTCTG ATGTCCTCGT GCCTCCTGTT GGAAACTACT TTGACCTGCC CCGGATCAGG
    CTGCCTCCAG GAATCATGAT AAAGCTCAGG GAAATTTCTG GGCGTGCTAG ACCACAGTTT
    AGACCAAGTA TAAAAGAAGT AATTCAGCCA GATGTGATGG AGGAAATGGT GGTATCATGT
    GTTATTAAGC ACTTGAACTT AGTTGATGCA CTGCAGTCCC TAATAAATTT CCAGTATCAA
    GAAGAACATG CTGAGGAATA TGATTTACTG TGTAAAATCA TGGGAGAGAC ATTTAAGAAA
    CTCAATGCCA TGGAGAGACA GCTGCAGAGT GTTGCAGAAT TGGAACAGAA ATGGCAGAGT
    GAAGTTGATG ATGCCATGCA GGGGAAACTG GAGAACAACA TGCCTTTCTT CTATGATTAC
    CATTTCAATG AGAACAAAAT GAAAGAACTA GAACTTTTGT GTTCAATGAA GGAAGTCTCC
    TTTGATGGAA ATGATCTTGA AAACATGGTG CTATCTCTGA GGGAGAAGTT CCTACAAGAA
    GTGAATTCTC TTATTCAGAA ACCCTCGCAC CCACTGGCTA AAACGAAGAC GTTAGTGAAG
    AGTCTAATGA ACCGAGCTGA GCTGTTGCTG CACGTCACCA TTGCAGCCCA GTCTGGCCTC
    ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA ACACCGGCTT GTAAATCAGC CTCTGAAACA
    AAGGTGATCT CTCATGCTGT GCGTCAGCCT GTTTTCCTTC GCAGCATGTC GGCTCCCTCT
    GACCTGGAAA TGATCGGTAA TGAAGATTTG GAATTTACTA GAGCAAATCA GAGGCGGCGC
    CACGTGACCA GCCACCGCAG CAGCTCCTTC ACACTCCTGC AGTCATTGGC CATAGAGGAC
    AGCAGGGACA AGCCCACCTA CAGCGTCCTG CTGGGGCAGC TGTTTGCTTT CATCGGCACC
    AACCCTGACC AAGCTGTGTC CAGCAGCAGC TTTCTTCTGG CCGCACAGAC GAGATGGCGG
    CGGGGAAACA CCCGCAAGCA GGCGTTGGTG CACATGCGGG AGCTGCTGAC GGCTGCCGTG
    CGAGTCGGGG GAGTGACACA CCTTGTGGGT CCAGTGACAA TGGTCCTGCA GGGAGGACCC
    AGAATTGAAG AACTGACCTG TGGTGGGATG GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG
    ACCATGACCT CTGTGGTGTC TTTGTGCGCA CGTTACCCTA TCGCATGTGC AAATAGCATC
    GGACTCTTGT GCACTATACC TTATACCAGG AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC
    CTTGTCCAGC TCATGGATCG ACTATGCAGC TTGAGCAGTC AGACTGAGTC CAGCTCCAGT
    GAGAAACAAA CCAAGAAGCA GAAGGTGGCC ACCATGGCCT GGGCAGCCTT CCAGGTGCTT
    GCCAACCGCT GCGTTGAATG GGAAAAAGAA GAAGGCGGCT CCACAGAGGC TGTGCACTCA
    GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC ACGAATCACC TTGCCCGAGC GACCGAGTGC
    TGTGGCAACC AGGCTGCCGG GAACGATGCC CTCCAGGACG TCCTGAGTCT CCTCAATGAT
    CTCTCGAGGA GTCATATAGG TAAAGCCATC CTGAGCCAGC CAGCTTGTGT GTCCAAACTC
    CTCTCCCTGC TACTTGACCA ACGCCCATCC CCAAAGCTGG TTCTTATCAT TCTTCAGCTG
    TGCCGGGCGG CACTCCCGCT GATGAGCGTA GAAGACTGTG GGAACGTGGA GCTCCCACCC
    TGGAGCTACT CCGTCCCCTC CCTAAACAGC GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC
    AAGATCGCCT CGCTGCTCTT GGCAAAGCTG GCAGACTACG TGGTCCCAGG ATGTCAGACA
    GTCCTTTCTC CAACCGCTTC CGAACCTGAC ACCACATTGA CAAAAACCAG CCCCAAGAAT
    TCCTTAAAAG GCGATAAAGA TCCTGGAGAA GAGAGTGAAG CAGTGGATGG CAAGCTCTCT
    ATCTTCATCC ACAAGCGGGA AGACCAGTCG TCCCATGAAG TTCTGCAGCC ATTACTAAGT
    AGCTCAGAAG GACGGCCCTT CCGACTTGGT ACTGGTGCCA ACATGGAGAA AGTTGTGAAA
    ATGGACCGAG ACATGACGAA GGGTGGCTGC TGTGAGGTGA TTACGGAAGA GGCGGCAGCT
    GCTCTGCGAA AAGCGACCAA GTGGGCACAG TCAGGCCTCA TTGTCAGTGT TGGGCCACCC
    GTGGAGTCCC TCAACCCAGA GGCCGTGAGC GGGCTGTCCA CAGGGGACAA GAAGAAGACT
    GCCCAAACCT CCATTTGCCG AGAGAGAAAC TCTGAACTCG CCAGGACCGA CCCCGTCCGA
    CCTTTCATCA GTGGGCACGT GGCAAACAGC ATGGCTGCTG AGGTGATCGC CTTGCTTCAT
    AGCTTGCTGA TGGCACCTGA GTCAAATGCT GCTCAGATAT GGACCACGAC AGCAGAGAAG
    GTTCTGTCTC GTGCACTGAT GTACATTCCA CAGTTGGGGA AATATGCAGA AAGCATTCTG
    GAAAATGGCA GCAGCAGTGG CAGAAAGCTT GCCAAACTTC AGAGAATTGC CCGCCAGGCT
    GTGGCTGCAC TCTGTGCACT TGGAGGCTTC AAAGAGACAA TCAAGATCGG GTCTGAGGTC
    CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC ATCGGAGTGG TGGCTTCTAT CAACGAACAG
    GAAGGTATAG CTACGGTCAG ATTCCCACCC ATAGACTGTA GAAAGACTTC GCAAGCATCA
    GACACACTGA CTATTCCATT GTCTAGACTT TGTGTTCCAA GATCAGAGGC ATTGCCTCTT
    CATAAACTGT CCATTACTGA GAAGGTAGTA CAGGCAGTCC AGTCCATGTT GCTTCCTCAG
    GAGGGAAGTC TCTCTATTCA TACCTCACTT CCTGCAGCAG GAGATGGGTC AGCTCCTGTG
    ATGGCAGTCG TTCGGCTCCT GGCTGAAATA AGAACAAGAG CATGCCTGGT CATGGCCCAG
    CTTTTAGAAG ACAGCTTATT TTGTGAAGAA TTCATACAGC AGTGTCCAGC AGCTGTTGAA
    GTTCTTAACC TAGTAGCCCA GGAATGCAGT GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG
    CAGTGTGAAC GGCTGAGGAT GCTCTACCGG GACTGTGCTC GGCCCCCGCC GCCTCCGCTG
    CAGGCTGACC GGAGACAGCC CAAAGAGATT ACGTGGAGCC CCTCACGAGT GTTTCCACCT
    GTCCGAGCCT GCATGTTCTC CTCGCACCTC ACTTCTGTCA CCTTCCTAGC AGACCCCAGT
    GCAGGGGGCG GTCTGCCCAG GGGCACATTT ATCTACGCCA CCTCACCACT GCCCGTTCAG
    GCCCCGTCTT TCTACTGGGA AATTGAAATT GTTTCCTATG GAGATACAGA TGATGATACT
    GGACCAATAG TTTCCTTTGG CTTTGCTACA GAAGCAGAGA AAAGAGATGG GGCATGGACT
    AATCCAGTGG GCACCTGTCT TTTCCATAAC AATGGCCGAG CTGTGCATTA CAATGGCTCC
    AGTTTATTAC AGTGGAAGAG CGTTCGATTA GACGTAACTC TCTCTCCTGG TGACGTGGCA
    GGGATTGGCT GGGAGAGAAC CGAGGGGACG CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC
    CGTGTGTACT TCACATACTG TGGGCAGCGC CTCTCACCGT ATCTCGAAGA TGTCTCGGGT
    GGGATGTGGC CGGTGGTTCA TATTCAGAAA AAGAACACCA AGACTCGCGC TAATTTTGGC
    TCCCGCCCAT TTGCCTACGC TGAAGGGCAA GCCCACCGCA ATGCTGCCGA CCTGTGCACT
    GACCTGGCCG AGGAAATTAG CGCTAACTTC GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC
    AGTGATAACG ATGCTGGCAC CAGTATTGCG TCAGACCCGG GCACTCATGG GCCACCATGC
    CGAATTGCTG CCGTAGCCAC TGCTCAGCAA CAATATGATA GTGATACTTC CTGTCATTAT
    AAAGTGGAGC TCAGCTATGA GAATTTCATC ACCTCTGGGC CAGACCCTCA CCCACCCCCC
    ATTGCAGATG ACGAAAGTGA TGATGATGAT GACGATGACA TCCCACAGGA GGATCACTAT
    GCCCTGCTGG TGAAAGCCTG GGAGACCAAG GTTTTTCCTA CCATCCGAAG ACGGTTCCGC
    AACGAAGCAG AGAGGAAATC AGGACTGGAC CAGATAAAGG GTGCTTTACA ACTAGGCATG
    GTGGATATTG CCCGGCAGAC AGTTGAATTC CTCTACGAGG AGAATGGTGG CATCCCAAGA
    GACCTTTATC TTCCCACCAT TGAAGACATT AAAGATGAAG CGAACAAGTT CACAATTGAT
    AAAGTTCGAA AAGGTCTCAC GGTAGTAACA CGCTCTCCAG ACAGCAATAA CGTAGCCAGC
    AGCACTGTTG GAACTGCCCT GCCAAAATTT GCTATCCGAG GGATGCTGAA AACCTTTGGG
    CTTCATGGAG TTGTCTTAGA TGTTGATTCA GTTAATGAAC TAGTTCAGGT AGAAACGTAC
    CTCCGAAGTG AAGGTGTGCT GGTCCGATAC TGGTACCCTA TTGATATGTT GGAAAGGCCC
    CCCGCAGGCT ACAGAAGGAC TGCCACCAAT GGGCTCGTCA CACTGGACAA TACCAACCTT
    CAAATTCACA GGGAGCTGCT GCGCTGCGAG GCCGCGCTGG CCAGGCTGTA CTGCCGCATG
    GCCCTGCTCA ACATCTTCGC CCCCAAGCTG CCTCACCTGT TTACCCGCCT CTTCCACATC
    CCCGCCATCC GGGACATTAC CCTGGAGCAC CTGCAGCTGC TGTCCAATCA GCTCCTCGCT
    CCTCCCCTCC CAGATGGCAC CATCAGCTCC AGCTCCATCC TCCTGGCGCA GTCCCTGCAG
    CACTGTGTCC ATTCCCAGAA CTGTTCGGCC ACAGACCTCT TCTACCAGGG CAGCTCCCAG
    ACGGTGCGAG AGTGGCTCAA CGTGGCCATC ACGCGGACCC TGCACCAGGG CGAGGAAAGC
    CTTCTGGAGC TGACCAAACA GATCTGCTCT TTCCTGCAGA CAGCACCAGA ACAGTTCCCC
    TCCGAAGAGT TTCCAATTTC CGAGTCCAAA GTCAACATGG ACGTCAATTT CCCCGGGGCA
    GCTTTCGTCG TCGTATCTTG CAAAGAAAGT CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG
    TACAAGGCAC CATGGGCGCG TGTGCTGGTG TACGGGCTAG GCCACAAAGT GAAGCGAAAT
    GGCCAGCTGA ACCTCATTGA GGCCGTCTGT TACCCGCGGG ATGCGTCTCC GGCCAACACA
    GGGCTCACAC CACCTCCTAC CACCAACCAG TACCCTTCTG TGATCCTCTC CACAGACAAA
    GTCCACATCA AACTGGGGGT ATCTCCCCCA CCCGGAGCCG TGCTGGTGTT ACACTCCCTG
    CCTCTCGAGT TCCCCCTGGC GATGGCCTTC ACCGAGCAGC TACTGTCCTG GAAATCAGAG
    GATGGCGAAG GTAGAGCTGA AGATGAGCCT GACACCATCC CGACGTCCGT CCTCCTGCAA
    GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG ACCACAGACA TGGCGGCCTG CGTGAAGGAG
    CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG CGCACGGTGC ACAGCCTAGA GCAGAGGAAG
    CACCCAGCCG GCCTGTCCTC ATCCATCGCC CTCCAGCTGA ACCCCTGCCT GGCCATGCTG
    ATGGCCCTGC AGTCGGAGCT TCACAAGCTG TACGACGAGG AGACGCAGAG CTGGGTCTCG
    GGCAGTGCAT GCGGAGGCTC AGGAGCCACG GCGGCTGCTG ACCAGGGCCG GTTCTCGACG
    TATTTCCACG CACTCATGGA GGGCTGCCTG GCGGTGGCCG AGGTGACCCT GCCCACCAAC
    ATGAGTGTCA CCGCCAGCGG GGTGACGTCA GCAACTGCCC CAAACCTCAG CGACTCGTCC
    TCCTCCTCAT CGTCCTCCCC AGGACAGACT CCACAGAGCC CCAGCCTCCT GTCCAAGAGG
    AAGAAAGTCA AGATGAAGCG GGAAAAGGCC TCCTCCTCTG GGAAGCGCCA GTCCTCACGC
    TCTGTGGACT CGGACCCCGC CGTGCTGAGC ATCGGGGGCA GCAAGCCCGA GGACATGCTG
    TGGTTCCATC GTGCTCTGAC GTTGCTCATC ATCCTCCGCC ACCTCACCAG GAAGGACCCA
    CAGGGGCTAG GCGTGACAAG CGACGCCATC GCCGACGCCT GCCAGGCCCT GGTGGGCCCC
    ACCGCCCACA GCCGCTTGCT GGTGATCTCT GGGATCCCCA CCCACCTGGA CGAGGGCATC
    GTCAGGGGTG CCATCCGCAA GGCCTGCAAT GCCCATGGCG GGGTGTTCAA GGATGAGATC
    TACATCCCCC TGCAGGACGA AGACCCCAAG AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT
    GGGAAAGCCG AGCCGGAAAA GACCCTCGGC TTCCCCAGCA CGGACAGCTT GGAGGTCAGC
    ACATCCAGCA GCCTGACCCC CGCTATGAGC GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT
    TCCATCTGCA GCTCCCAGGG CATCTCGCAG ACAGTCAGTG ACCTCTCTGC GGATCCGTTG
    CCTGCAGGCC TGGAGCTGCC CATCCCTGCA GGCTTGTTGG AGCCCCATGT GGTGTCCAGC
    CAGGAGAGCC TGGACATTTC CCTGTGTAGC ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG
    GGCGAGCCAC TGGACAATGC AGAAACGGCC TCAGTGTCAG ACATGGGCTC CATGTACACA
    GTCACGTCCC TGGACAACCA GCCCCTTGCA GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG
    GAGATAAGGT CCCGAGCCAA AATTGAGAAA ATTCGAGCAA GTTTATTTAA CAACAATGAC
    TTGATAGGTT TGTCAAGCTT GGATGGTGAG GATGAATTGA TGGAAATGTC AACGGAAGAG
    ATCCTCACGG TGTCTGTAGT GAATCAGAGT CTATTCGATA CGCAGGGGAG CCCAGGGTTA
    GAAGATTATT TCAATGATAA GTCAATTAAA GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG
    GTGCTGACGG AGATATTTAA GAGCTGTGCC CACTCGGAGC AGATGCTGAG CCTGACGCCA
    GCAAAGCCCA TCAAAGTGTC GGACATTTAT CTTAGCAAAG AGCAGATCAA CTCCCAGACA
    CCGGGCAACC TCCTCCACCT TTTCTTCACC AATGTCCGGC CCCCCAAAAA GGTGCTAGAG
    GACCAGCTCA CCCAGATCCT GAGGAAGTAC GGTGTGCCGA AGCCCAAGTT TGACAAGAGC
    AAGTACAGCA AGGCGGGGAA GGAGCAGCAC CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC
    ATCACCAAGC CGCCCACCAA GGACAAGGCA GTGCTCAACA GCGTCAGCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52730
    Clone ID Related Accession (Same CDS sequence) XM_010814058.2 , XM_015467163.1
    Accession Version XM_015467163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
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    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1981
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    10501
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    11101
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    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATCTAAC AGTATATTTT CTGTTTTGAA GTTGGTGATA
    TACGCGGGGC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGA AGTGGCCGTG AACACAACAC TCCCGATAAG
    GCTATGTGGG GCTTTGCTTG CACAGTTCGC GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA
    GGCTTACCCT TTCTGGTAGA CCTGGCTTTA GGTCTATCTG TGTTAGCTTG TTCCATGTTA
    AGAATCCTAT ACAACGGACC AGAAATTACC AAAGAAGAAG AAACCTGTCA GGAGCTGTTG
    CGGTCCAAAC TTTTACAAAG GTGCCAGTGG CAGGTAGAGG CCAACGGTGT GATCTCCCCT
    GCCCTTACTC CAAGCCCATC TCCACTGCCT CTGACCATCG AGGAAGACAG AGAGTTCACT
    TACCCGTCTG ATGTCCTCGT GCCTCCTGTT GGAAACTACT TTGACCTGCC CCGGATCAGG
    CTGCCTCCAG GAATCATGAT AAAGCTCAGG GAAATTTCTG GGCGTGCTAG ACCACAGTTT
    AGACCAAGTA TAAAAGAAGT AATTCAGCCA GATGTGATGG AGGAAATGGT GGTATCATGT
    GTTATTAAGC ACTTGAACTT AGTTGATGCA CTGCAGTCCC TAATAAATTT CCAGTATCAA
    GAAGAACATG CTGAGGAATA TGATTTACTG TGTAAAATCA TGGGAGAGAC ATTTAAGAAA
    CTCAATGCCA TGGAGAGACA GCTGCAGAGT GTTGCAGAAT TGGAACAGAA ATGGCAGAGT
    GAAGTTGATG ATGCCATGCA GGGGAAACTG GAGAACAACA TGCCTTTCTT CTATGATTAC
    CATTTCAATG AGAACAAAAT GAAAGAACTA GAACTTTTGT GTTCAATGAA GGAAGTCTCC
    TTTGATGGAA ATGATCTTGA AAACATGGTG CTATCTCTGA GGGAGAAGTT CCTACAAGAA
    GTGAATTCTC TTATTCAGAA ACCCTCGCAC CCACTGGCTA AAACGAAGAC GTTAGTGAAG
    AGTCTAATGA ACCGAGCTGA GCTGTTGCTG CACGTCACCA TTGCAGCCCA GTCTGGCCTC
    ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA ACACCGGCTT GTAAATCAGC CTCTGAAACA
    AAGGTGATCT CTCATGCTGT GCGTCAGCCT GTTTTCCTTC GCAGCATGTC GGCTCCCTCT
    GACCTGGAAA TGATCGGTAA TGAAGATTTG GAATTTACTA GAGCAAATCA GAGGCGGCGC
    CACGTGACCA GCCACCGCAG CAGCTCCTTC ACACTCCTGC AGTCATTGGC CATAGAGGAC
    AGCAGGGACA AGCCCACCTA CAGCGTCCTG CTGGGGCAGC TGTTTGCTTT CATCGGCACC
    AACCCTGACC AAGCTGTGTC CAGCAGCAGC TTTCTTCTGG CCGCACAGAC GAGATGGCGG
    CGGGGAAACA CCCGCAAGCA GGCGTTGGTG CACATGCGGG AGCTGCTGAC GGCTGCCGTG
    CGAGTCGGGG GAGTGACACA CCTTGTGGGT CCAGTGACAA TGGTCCTGCA GGGAGGACCC
    AGAATTGAAG AACTGACCTG TGGTGGGATG GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG
    ACCATGACCT CTGTGGTGTC TTTGTGCGCA CGTTACCCTA TCGCATGTGC AAATAGCATC
    GGACTCTTGT GCACTATACC TTATACCAGG AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC
    CTTGTCCAGC TCATGGATCG ACTATGCAGC TTGAGCAGTC AGACTGAGTC CAGCTCCAGT
    GAGAAACAAA CCAAGAAGCA GAAGGTGGCC ACCATGGCCT GGGCAGCCTT CCAGGTGCTT
    GCCAACCGCT GCGTTGAATG GGAAAAAGAA GAAGGCGGCT CCACAGAGGC TGTGCACTCA
    GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC ACGAATCACC TTGCCCGAGC GACCGAGTGC
    TGTGGCAACC AGGCTGCCGG GAACGATGCC CTCCAGGACG TCCTGAGTCT CCTCAATGAT
    CTCTCGAGGA GTCATATAGG TAAAGCCATC CTGAGCCAGC CAGCTTGTGT GTCCAAACTC
    CTCTCCCTGC TACTTGACCA ACGCCCATCC CCAAAGCTGG TTCTTATCAT TCTTCAGCTG
    TGCCGGGCGG CACTCCCGCT GATGAGCGTA GAAGACTGTG GGAACGTGGA GCTCCCACCC
    TGGAGCTACT CCGTCCCCTC CCTAAACAGC GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC
    AAGATCGCCT CGCTGCTCTT GGCAAAGCTG GCAGACTACG TGGTCCCAGG ATGTCAGACA
    GTCCTTTCTC CAACCGCTTC CGAACCTGAC ACCACATTGA CAAAAACCAG CCCCAAGAAT
    TCCTTAAAAG GCGATAAAGA TCCTGGAGAA GAGAGTGAAG CAGTGGATGG CAAGCTCTCT
    ATCTTCATCC ACAAGCGGGA AGACCAGTCG TCCCATGAAG TTCTGCAGCC ATTACTAAGT
    AGCTCAGAAG GACGGCCCTT CCGACTTGGT ACTGGTGCCA ACATGGAGAA AGTTGTGAAA
    ATGGACCGAG ACATGACGAA GGGTGGCTGC TGTGAGGTGA TTACGGAAGA GGCGGCAGCT
    GCTCTGCGAA AAGCGACCAA GTGGGCACAG TCAGGCCTCA TTGTCAGTGT TGGGCCACCC
    GTGGAGTCCC TCAACCCAGA GGCCGTGAGC GGGCTGTCCA CAGGGGACAA GAAGAAGACT
    GCCCAAACCT CCATTTGCCG AGAGAGAAAC TCTGAACTCG CCAGGACCGA CCCCGTCCGA
    CCTTTCATCA GTGGGCACGT GGCAAACAGC ATGGCTGCTG AGGTGATCGC CTTGCTTCAT
    AGCTTGCTGA TGGCACCTGA GTCAAATGCT GCTCAGATAT GGACCACGAC AGCAGAGAAG
    GTTCTGTCTC GTGCACTGAT GTACATTCCA CAGTTGGGGA AATATGCAGA AAGCATTCTG
    GAAAATGGCA GCAGCAGTGG CAGAAAGCTT GCCAAACTTC AGAGAATTGC CCGCCAGGCT
    GTGGCTGCAC TCTGTGCACT TGGAGGCTTC AAAGAGACAA TCAAGATCGG GTCTGAGGTC
    CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC ATCGGAGTGG TGGCTTCTAT CAACGAACAG
    GAAGGTATAG CTACGGTCAG ATTCCCACCC ATAGACTGTA GAAAGACTTC GCAAGCATCA
    GACACACTGA CTATTCCATT GTCTAGACTT TGTGTTCCAA GATCAGAGGC ATTGCCTCTT
    CATAAACTGT CCATTACTGA GAAGGTAGTA CAGGCAGTCC AGTCCATGTT GCTTCCTCAG
    GAGGGAAGTC TCTCTATTCA TACCTCACTT CCTGCAGCAG GAGATGGGTC AGCTCCTGTG
    ATGGCAGTCG TTCGGCTCCT GGCTGAAATA AGAACAAGAG CATGCCTGGT CATGGCCCAG
    CTTTTAGAAG ACAGCTTATT TTGTGAAGAA TTCATACAGC AGTGTCCAGC AGCTGTTGAA
    GTTCTTAACC TAGTAGCCCA GGAATGCAGT GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG
    CAGTGTGAAC GGCTGAGGAT GCTCTACCGG GACTGTGCTC GGCCCCCGCC GCCTCCGCTG
    CAGGCTGACC GGAGACAGCC CAAAGAGATT ACGTGGAGCC CCTCACGAGT GTTTCCACCT
    GTCCGAGCCT GCATGTTCTC CTCGCACCTC ACTTCTGTCA CCTTCCTAGC AGACCCCAGT
    GCAGGGGGCG GTCTGCCCAG GGGCACATTT ATCTACGCCA CCTCACCACT GCCCGTTCAG
    GCCCCGTCTT TCTACTGGGA AATTGAAATT GTTTCCTATG GAGATACAGA TGATGATACT
    GGACCAATAG TTTCCTTTGG CTTTGCTACA GAAGCAGAGA AAAGAGATGG GGCATGGACT
    AATCCAGTGG GCACCTGTCT TTTCCATAAC AATGGCCGAG CTGTGCATTA CAATGGCTCC
    AGTTTATTAC AGTGGAAGAG CGTTCGATTA GACGTAACTC TCTCTCCTGG TGACGTGGCA
    GGGATTGGCT GGGAGAGAAC CGAGGGGACG CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC
    CGTGTGTACT TCACATACTG TGGGCAGCGC CTCTCACCGT ATCTCGAAGA TGTCTCGGGT
    GGGATGTGGC CGGTGGTTCA TATTCAGAAA AAGAACACCA AGACTCGCGC TAATTTTGGC
    TCCCGCCCAT TTGCCTACGC TGAAGGGCAA GCCCACCGCA ATGCTGCCGA CCTGTGCACT
    GACCTGGCCG AGGAAATTAG CGCTAACTTC GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC
    AGTGATAACG ATGCTGGCAC CAGTATTGCG TCAGACCCGG GCACTCATGG GCCACCATGC
    CGAATTGCTG CCGTAGCCAC TGCTCAGCAA CAATATGATA GTGATACTTC CTGTCATTAT
    AAAGTGGAGC TCAGCTATGA GAATTTCATC ACCTCTGGGC CAGACCCTCA CCCACCCCCC
    ATTGCAGATG ACGAAAGTGA TGATGATGAT GACGATGACA TCCCACAGGA GGATCACTAT
    GCCCTGCTGG TGAAAGCCTG GGAGACCAAG GTTTTTCCTA CCATCCGAAG ACGGTTCCGC
    AACGAAGCAG AGAGGAAATC AGGACTGGAC CAGATAAAGG GTGCTTTACA ACTAGGCATG
    GTGGATATTG CCCGGCAGAC AGTTGAATTC CTCTACGAGG AGAATGGTGG CATCCCAAGA
    GACCTTTATC TTCCCACCAT TGAAGACATT AAAGATGAAG CGAACAAGTT CACAATTGAT
    AAAGTTCGAA AAGGTCTCAC GGTAGTAACA CGCTCTCCAG ACAGCAATAA CGTAGCCAGC
    AGCACTGTTG GAACTGCCCT GCCAAAATTT GCTATCCGAG GGATGCTGAA AACCTTTGGG
    CTTCATGGAG TTGTCTTAGA TGTTGATTCA GTTAATGAAC TAGTTCAGGT AGAAACGTAC
    CTCCGAAGTG AAGGTGTGCT GGTCCGATAC TGGTACCCTA TTGATATGTT GGAAAGGCCC
    CCCGCAGGCT ACAGAAGGAC TGCCACCAAT GGGCTCGTCA CACTGGACAA TACCAACCTT
    CAAATTCACA GGGAGCTGCT GCGCTGCGAG GCCGCGCTGG CCAGGCTGTA CTGCCGCATG
    GCCCTGCTCA ACATCTTCGC CCCCAAGCTG CCTCACCTGT TTACCCGCCT CTTCCACATC
    CCCGCCATCC GGGACATTAC CCTGGAGCAC CTGCAGCTGC TGTCCAATCA GCTCCTCGCT
    CCTCCCCTCC CAGATGGCAC CATCAGCTCC AGCTCCATCC TCCTGGCGCA GTCCCTGCAG
    CACTGTGTCC ATTCCCAGAA CTGTTCGGCC ACAGACCTCT TCTACCAGGG CAGCTCCCAG
    ACGGTGCGAG AGTGGCTCAA CGTGGCCATC ACGCGGACCC TGCACCAGGG CGAGGAAAGC
    CTTCTGGAGC TGACCAAACA GATCTGCTCT TTCCTGCAGA CAGCACCAGA ACAGTTCCCC
    TCCGAAGAGT TTCCAATTTC CGAGTCCAAA GTCAACATGG ACGTCAATTT CCCCGGGGCA
    GCTTTCGTCG TCGTATCTTG CAAAGAAAGT CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG
    TACAAGGCAC CATGGGCGCG TGTGCTGGTG TACGGGCTAG GCCACAAAGT GAAGCGAAAT
    GGCCAGCTGA ACCTCATTGA GGCCGTCTGT TACCCGCGGG ATGCGTCTCC GGCCAACACA
    GGGCTCACAC CACCTCCTAC CACCAACCAG TACCCTTCTG TGATCCTCTC CACAGACAAA
    GTCCACATCA AACTGGGGGT ATCTCCCCCA CCCGGAGCCG TGCTGGTGTT ACACTCCCTG
    CCTCTCGAGT TCCCCCTGGC GATGGCCTTC ACCGAGCAGC TACTGTCCTG GAAATCAGAG
    GATGGCGAAG GTAGAGCTGA AGATGAGCCT GACACCATCC CGACGTCCGT CCTCCTGCAA
    GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG ACCACAGACA TGGCGGCCTG CGTGAAGGAG
    CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG CGCACGGTGC ACAGCCTAGA GCAGAGGAAG
    CACCCAGCCG GCCTGTCCTC ATCCATCGCC CTCCAGCTGA ACCCCTGCCT GGCCATGCTG
    ATGGCCCTGC AGTCGGAGCT TCACAAGCTG TACGACGAGG AGACGCAGAG CTGGGTCTCG
    GGCAGTGCAT GCGGAGGCTC AGGAGCCACG GCGGCTGCTG ACCAGGGCCG GTTCTCGACG
    TATTTCCACG CACTCATGGA GGGCTGCCTG GCGGTGGCCG AGGTGACCCT GCCCACCAAC
    ATGAGTGTCA CCGCCAGCGG GGTGACGTCA GCAACTGCCC CAAACCTCAG CGACTCGTCC
    TCCTCCTCAT CGTCCTCCCC AGGACAGACT CCACAGAGCC CCAGCCTCCT GTCCAAGAGG
    AAGAAAGTCA AGATGAAGCG GGAAAAGGCC TCCTCCTCTG GGAAGCGCCA GTCCTCACGC
    TCTGTGGACT CGGACCCCGC CGTGCTGAGC ATCGGGGGCA GCAAGCCCGA GGACATGCTG
    TGGTTCCATC GTGCTCTGAC GTTGCTCATC ATCCTCCGCC ACCTCACCAG GAAGGACCCA
    CAGGGGCTAG GCGTGACAAG CGACGCCATC GCCGACGCCT GCCAGGCCCT GGTGGGCCCC
    ACCGCCCACA GCCGCTTGCT GGTGATCTCT GGGATCCCCA CCCACCTGGA CGAGGGCATC
    GTCAGGGGTG CCATCCGCAA GGCCTGCAAT GCCCATGGCG GGGTGTTCAA GGATGAGATC
    TACATCCCCC TGCAGGACGA AGACCCCAAG AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT
    GGGAAAGCCG AGCCGGAAAA GACCCTCGGC TTCCCCAGCA CGGACAGCTT GGAGGTCAGC
    ACATCCAGCA GCCTGACCCC CGCTATGAGC GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT
    TCCATCTGCA GCTCCCAGGG CATCTCGCAG ACAGTCAGTG ACCTCTCTGC GGATCCGTTG
    CCTGCAGGCC TGGAGCTGCC CATCCCTGCA GGCTTGTTGG AGCCCCATGT GGTGTCCAGC
    CAGGAGAGCC TGGACATTTC CCTGTGTAGC ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG
    GGCGAGCCAC TGGACAATGC AGAAACGGCC TCAGTGTCAG ACATGGGCTC CATGTACACA
    GTCACGTCCC TGGACAACCA GCCCCTTGCA GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG
    GAGATAAGGT CCCGAGCCAA AATTGAGAAA ATTCGAGCAA GTTTATTTAA CAACAATGAC
    TTGATAGGTT TGTCAAGCTT GGATGGTGAG GATGAATTGA TGGAAATGTC AACGGAAGAG
    ATCCTCACGG TGTCTGTAGT GAATCAGAGT CTATTCGATA CGCAGGGGAG CCCAGGGTTA
    GAAGATTATT TCAATGATAA GTCAATTAAA GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG
    GTGCTGACGG AGATATTTAA GAGCTGTGCC CACTCGGAGC AGATGCTGAG CCTGACGCCA
    GCAAAGCCCA TCAAAGTGTC GGACATTTAT CTTAGCAAAG AGCAGATCAA CTCCCAGACA
    CCGGGCAACC TCCTCCACCT TTTCTTCACC AATGTCCGGC CCCCCAAAAA GGTGCTAGAG
    GACCAGCTCA CCCAGATCCT GAGGAAGTAC GGTGTGCCGA AGCCCAAGTT TGACAAGAGC
    AAGTACAGCA AGGCGGGGAA GGAGCAGCAC CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC
    ATCACCAAGC CGCCCACCAA GGACAAGGCA GTGCTCAACA GCGTCAGCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322649.1
    CDS1026..12746
    Translation

    Target ORF information:

    RefSeq Version XM_015467163.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATCTAAC AGTATATTTT CTGTTTTGAA GTTGGTGATA
    TACGCGGGGC CTAACACAAA CAGTAGGAAG GTTGCTGAGT ATGGAGGCAA TACACTGGGA
    TATGGCAGCC GTAGTGTCTT AGGAACTGGT TGGCCGAAAG ACTTGGTGAA GGTGGAAGGA
    GATACAGTCA CCTTCTCCTT TGAAATGAGA AGTGGCCGTG AACACAACAC TCCCGATAAG
    GCTATGTGGG GCTTTGCTTG CACAGTTCGC GCTCAGGAGT CTTCGGAGGA TGTGTCAGGA
    GGCTTACCCT TTCTGGTAGA CCTGGCTTTA GGTCTATCTG TGTTAGCTTG TTCCATGTTA
    AGAATCCTAT ACAACGGACC AGAAATTACC AAAGAAGAAG AAACCTGTCA GGAGCTGTTG
    CGGTCCAAAC TTTTACAAAG GTGCCAGTGG CAGGTAGAGG CCAACGGTGT GATCTCCCCT
    GCCCTTACTC CAAGCCCATC TCCACTGCCT CTGACCATCG AGGAAGACAG AGAGTTCACT
    TACCCGTCTG ATGTCCTCGT GCCTCCTGTT GGAAACTACT TTGACCTGCC CCGGATCAGG
    CTGCCTCCAG GAATCATGAT AAAGCTCAGG GAAATTTCTG GGCGTGCTAG ACCACAGTTT
    AGACCAAGTA TAAAAGAAGT AATTCAGCCA GATGTGATGG AGGAAATGGT GGTATCATGT
    GTTATTAAGC ACTTGAACTT AGTTGATGCA CTGCAGTCCC TAATAAATTT CCAGTATCAA
    GAAGAACATG CTGAGGAATA TGATTTACTG TGTAAAATCA TGGGAGAGAC ATTTAAGAAA
    CTCAATGCCA TGGAGAGACA GCTGCAGAGT GTTGCAGAAT TGGAACAGAA ATGGCAGAGT
    GAAGTTGATG ATGCCATGCA GGGGAAACTG GAGAACAACA TGCCTTTCTT CTATGATTAC
    CATTTCAATG AGAACAAAAT GAAAGAACTA GAACTTTTGT GTTCAATGAA GGAAGTCTCC
    TTTGATGGAA ATGATCTTGA AAACATGGTG CTATCTCTGA GGGAGAAGTT CCTACAAGAA
    GTGAATTCTC TTATTCAGAA ACCCTCGCAC CCACTGGCTA AAACGAAGAC GTTAGTGAAG
    AGTCTAATGA ACCGAGCTGA GCTGTTGCTG CACGTCACCA TTGCAGCCCA GTCTGGCCTC
    ACGAGAAGCA TCTCAGGGAC CCCCGCGGAA ACACCGGCTT GTAAATCAGC CTCTGAAACA
    AAGGTGATCT CTCATGCTGT GCGTCAGCCT GTTTTCCTTC GCAGCATGTC GGCTCCCTCT
    GACCTGGAAA TGATCGGTAA TGAAGATTTG GAATTTACTA GAGCAAATCA GAGGCGGCGC
    CACGTGACCA GCCACCGCAG CAGCTCCTTC ACACTCCTGC AGTCATTGGC CATAGAGGAC
    AGCAGGGACA AGCCCACCTA CAGCGTCCTG CTGGGGCAGC TGTTTGCTTT CATCGGCACC
    AACCCTGACC AAGCTGTGTC CAGCAGCAGC TTTCTTCTGG CCGCACAGAC GAGATGGCGG
    CGGGGAAACA CCCGCAAGCA GGCGTTGGTG CACATGCGGG AGCTGCTGAC GGCTGCCGTG
    CGAGTCGGGG GAGTGACACA CCTTGTGGGT CCAGTGACAA TGGTCCTGCA GGGAGGACCC
    AGAATTGAAG AACTGACCTG TGGTGGGATG GTCGAGCAGG TCCAGGAAGC CTTCGGTGAG
    ACCATGACCT CTGTGGTGTC TTTGTGCGCA CGTTACCCTA TCGCATGTGC AAATAGCATC
    GGACTCTTGT GCACTATACC TTATACCAGG AGTGAAGAGA AGTGCCTGGT GCGGAGTGGC
    CTTGTCCAGC TCATGGATCG ACTATGCAGC TTGAGCAGTC AGACTGAGTC CAGCTCCAGT
    GAGAAACAAA CCAAGAAGCA GAAGGTGGCC ACCATGGCCT GGGCAGCCTT CCAGGTGCTT
    GCCAACCGCT GCGTTGAATG GGAAAAAGAA GAAGGCGGCT CCACAGAGGC TGTGCACTCA
    GGTCTGGCCC GTCAGGTGTC CAGCCTTCTC ACGAATCACC TTGCCCGAGC GACCGAGTGC
    TGTGGCAACC AGGCTGCCGG GAACGATGCC CTCCAGGACG TCCTGAGTCT CCTCAATGAT
    CTCTCGAGGA GTCATATAGG TAAAGCCATC CTGAGCCAGC CAGCTTGTGT GTCCAAACTC
    CTCTCCCTGC TACTTGACCA ACGCCCATCC CCAAAGCTGG TTCTTATCAT TCTTCAGCTG
    TGCCGGGCGG CACTCCCGCT GATGAGCGTA GAAGACTGTG GGAACGTGGA GCTCCCACCC
    TGGAGCTACT CCGTCCCCTC CCTAAACAGC GAGCAGGAGG ATCCCAGCGA CCCAGCGTCC
    AAGATCGCCT CGCTGCTCTT GGCAAAGCTG GCAGACTACG TGGTCCCAGG ATGTCAGACA
    GTCCTTTCTC CAACCGCTTC CGAACCTGAC ACCACATTGA CAAAAACCAG CCCCAAGAAT
    TCCTTAAAAG GCGATAAAGA TCCTGGAGAA GAGAGTGAAG CAGTGGATGG CAAGCTCTCT
    ATCTTCATCC ACAAGCGGGA AGACCAGTCG TCCCATGAAG TTCTGCAGCC ATTACTAAGT
    AGCTCAGAAG GACGGCCCTT CCGACTTGGT ACTGGTGCCA ACATGGAGAA AGTTGTGAAA
    ATGGACCGAG ACATGACGAA GGGTGGCTGC TGTGAGGTGA TTACGGAAGA GGCGGCAGCT
    GCTCTGCGAA AAGCGACCAA GTGGGCACAG TCAGGCCTCA TTGTCAGTGT TGGGCCACCC
    GTGGAGTCCC TCAACCCAGA GGCCGTGAGC GGGCTGTCCA CAGGGGACAA GAAGAAGACT
    GCCCAAACCT CCATTTGCCG AGAGAGAAAC TCTGAACTCG CCAGGACCGA CCCCGTCCGA
    CCTTTCATCA GTGGGCACGT GGCAAACAGC ATGGCTGCTG AGGTGATCGC CTTGCTTCAT
    AGCTTGCTGA TGGCACCTGA GTCAAATGCT GCTCAGATAT GGACCACGAC AGCAGAGAAG
    GTTCTGTCTC GTGCACTGAT GTACATTCCA CAGTTGGGGA AATATGCAGA AAGCATTCTG
    GAAAATGGCA GCAGCAGTGG CAGAAAGCTT GCCAAACTTC AGAGAATTGC CCGCCAGGCT
    GTGGCTGCAC TCTGTGCACT TGGAGGCTTC AAAGAGACAA TCAAGATCGG GTCTGAGGTC
    CAGGTTTTAG GTAGAGGAAT ATCAGGAAGC ATCGGAGTGG TGGCTTCTAT CAACGAACAG
    GAAGGTATAG CTACGGTCAG ATTCCCACCC ATAGACTGTA GAAAGACTTC GCAAGCATCA
    GACACACTGA CTATTCCATT GTCTAGACTT TGTGTTCCAA GATCAGAGGC ATTGCCTCTT
    CATAAACTGT CCATTACTGA GAAGGTAGTA CAGGCAGTCC AGTCCATGTT GCTTCCTCAG
    GAGGGAAGTC TCTCTATTCA TACCTCACTT CCTGCAGCAG GAGATGGGTC AGCTCCTGTG
    ATGGCAGTCG TTCGGCTCCT GGCTGAAATA AGAACAAGAG CATGCCTGGT CATGGCCCAG
    CTTTTAGAAG ACAGCTTATT TTGTGAAGAA TTCATACAGC AGTGTCCAGC AGCTGTTGAA
    GTTCTTAACC TAGTAGCCCA GGAATGCAGT GCTGGAGAGC GGCTGGCAGT TGTGGAAGTG
    CAGTGTGAAC GGCTGAGGAT GCTCTACCGG GACTGTGCTC GGCCCCCGCC GCCTCCGCTG
    CAGGCTGACC GGAGACAGCC CAAAGAGATT ACGTGGAGCC CCTCACGAGT GTTTCCACCT
    GTCCGAGCCT GCATGTTCTC CTCGCACCTC ACTTCTGTCA CCTTCCTAGC AGACCCCAGT
    GCAGGGGGCG GTCTGCCCAG GGGCACATTT ATCTACGCCA CCTCACCACT GCCCGTTCAG
    GCCCCGTCTT TCTACTGGGA AATTGAAATT GTTTCCTATG GAGATACAGA TGATGATACT
    GGACCAATAG TTTCCTTTGG CTTTGCTACA GAAGCAGAGA AAAGAGATGG GGCATGGACT
    AATCCAGTGG GCACCTGTCT TTTCCATAAC AATGGCCGAG CTGTGCATTA CAATGGCTCC
    AGTTTATTAC AGTGGAAGAG CGTTCGATTA GACGTAACTC TCTCTCCTGG TGACGTGGCA
    GGGATTGGCT GGGAGAGAAC CGAGGGGACG CCTCCTCCAC CCGGGCAGCC TGCCAAGGGC
    CGTGTGTACT TCACATACTG TGGGCAGCGC CTCTCACCGT ATCTCGAAGA TGTCTCGGGT
    GGGATGTGGC CGGTGGTTCA TATTCAGAAA AAGAACACCA AGACTCGCGC TAATTTTGGC
    TCCCGCCCAT TTGCCTACGC TGAAGGGCAA GCCCACCGCA ATGCTGCCGA CCTGTGCACT
    GACCTGGCCG AGGAAATTAG CGCTAACTTC GAGGCCCTGC CCTTTGCCAT GGCCTCTGAC
    AGTGATAACG ATGCTGGCAC CAGTATTGCG TCAGACCCGG GCACTCATGG GCCACCATGC
    CGAATTGCTG CCGTAGCCAC TGCTCAGCAA CAATATGATA GTGATACTTC CTGTCATTAT
    AAAGTGGAGC TCAGCTATGA GAATTTCATC ACCTCTGGGC CAGACCCTCA CCCACCCCCC
    ATTGCAGATG ACGAAAGTGA TGATGATGAT GACGATGACA TCCCACAGGA GGATCACTAT
    GCCCTGCTGG TGAAAGCCTG GGAGACCAAG GTTTTTCCTA CCATCCGAAG ACGGTTCCGC
    AACGAAGCAG AGAGGAAATC AGGACTGGAC CAGATAAAGG GTGCTTTACA ACTAGGCATG
    GTGGATATTG CCCGGCAGAC AGTTGAATTC CTCTACGAGG AGAATGGTGG CATCCCAAGA
    GACCTTTATC TTCCCACCAT TGAAGACATT AAAGATGAAG CGAACAAGTT CACAATTGAT
    AAAGTTCGAA AAGGTCTCAC GGTAGTAACA CGCTCTCCAG ACAGCAATAA CGTAGCCAGC
    AGCACTGTTG GAACTGCCCT GCCAAAATTT GCTATCCGAG GGATGCTGAA AACCTTTGGG
    CTTCATGGAG TTGTCTTAGA TGTTGATTCA GTTAATGAAC TAGTTCAGGT AGAAACGTAC
    CTCCGAAGTG AAGGTGTGCT GGTCCGATAC TGGTACCCTA TTGATATGTT GGAAAGGCCC
    CCCGCAGGCT ACAGAAGGAC TGCCACCAAT GGGCTCGTCA CACTGGACAA TACCAACCTT
    CAAATTCACA GGGAGCTGCT GCGCTGCGAG GCCGCGCTGG CCAGGCTGTA CTGCCGCATG
    GCCCTGCTCA ACATCTTCGC CCCCAAGCTG CCTCACCTGT TTACCCGCCT CTTCCACATC
    CCCGCCATCC GGGACATTAC CCTGGAGCAC CTGCAGCTGC TGTCCAATCA GCTCCTCGCT
    CCTCCCCTCC CAGATGGCAC CATCAGCTCC AGCTCCATCC TCCTGGCGCA GTCCCTGCAG
    CACTGTGTCC ATTCCCAGAA CTGTTCGGCC ACAGACCTCT TCTACCAGGG CAGCTCCCAG
    ACGGTGCGAG AGTGGCTCAA CGTGGCCATC ACGCGGACCC TGCACCAGGG CGAGGAAAGC
    CTTCTGGAGC TGACCAAACA GATCTGCTCT TTCCTGCAGA CAGCACCAGA ACAGTTCCCC
    TCCGAAGAGT TTCCAATTTC CGAGTCCAAA GTCAACATGG ACGTCAATTT CCCCGGGGCA
    GCTTTCGTCG TCGTATCTTG CAAAGAAAGT CAGTCTGGAT TCCGCAAAGA TTCCTCTCTG
    TACAAGGCAC CATGGGCGCG TGTGCTGGTG TACGGGCTAG GCCACAAAGT GAAGCGAAAT
    GGCCAGCTGA ACCTCATTGA GGCCGTCTGT TACCCGCGGG ATGCGTCTCC GGCCAACACA
    GGGCTCACAC CACCTCCTAC CACCAACCAG TACCCTTCTG TGATCCTCTC CACAGACAAA
    GTCCACATCA AACTGGGGGT ATCTCCCCCA CCCGGAGCCG TGCTGGTGTT ACACTCCCTG
    CCTCTCGAGT TCCCCCTGGC GATGGCCTTC ACCGAGCAGC TACTGTCCTG GAAATCAGAG
    GATGGCGAAG GTAGAGCTGA AGATGAGCCT GACACCATCC CGACGTCCGT CCTCCTGCAA
    GTGGTGGAGC TGCTTGGCAA CTTCCTCTGG ACCACAGACA TGGCGGCCTG CGTGAAGGAG
    CTGGTTTTCC ACCTCCTGGC AGAGCTCCTG CGCACGGTGC ACAGCCTAGA GCAGAGGAAG
    CACCCAGCCG GCCTGTCCTC ATCCATCGCC CTCCAGCTGA ACCCCTGCCT GGCCATGCTG
    ATGGCCCTGC AGTCGGAGCT TCACAAGCTG TACGACGAGG AGACGCAGAG CTGGGTCTCG
    GGCAGTGCAT GCGGAGGCTC AGGAGCCACG GCGGCTGCTG ACCAGGGCCG GTTCTCGACG
    TATTTCCACG CACTCATGGA GGGCTGCCTG GCGGTGGCCG AGGTGACCCT GCCCACCAAC
    ATGAGTGTCA CCGCCAGCGG GGTGACGTCA GCAACTGCCC CAAACCTCAG CGACTCGTCC
    TCCTCCTCAT CGTCCTCCCC AGGACAGACT CCACAGAGCC CCAGCCTCCT GTCCAAGAGG
    AAGAAAGTCA AGATGAAGCG GGAAAAGGCC TCCTCCTCTG GGAAGCGCCA GTCCTCACGC
    TCTGTGGACT CGGACCCCGC CGTGCTGAGC ATCGGGGGCA GCAAGCCCGA GGACATGCTG
    TGGTTCCATC GTGCTCTGAC GTTGCTCATC ATCCTCCGCC ACCTCACCAG GAAGGACCCA
    CAGGGGCTAG GCGTGACAAG CGACGCCATC GCCGACGCCT GCCAGGCCCT GGTGGGCCCC
    ACCGCCCACA GCCGCTTGCT GGTGATCTCT GGGATCCCCA CCCACCTGGA CGAGGGCATC
    GTCAGGGGTG CCATCCGCAA GGCCTGCAAT GCCCATGGCG GGGTGTTCAA GGATGAGATC
    TACATCCCCC TGCAGGACGA AGACCCCAAG AAGCCAAAAG ATAAGGCCGA GGGGGGTGAT
    GGGAAAGCCG AGCCGGAAAA GACCCTCGGC TTCCCCAGCA CGGACAGCTT GGAGGTCAGC
    ACATCCAGCA GCCTGACCCC CGCTATGAGC GTCAGCGCAT CCGCGTCCAC CAGCCAGGCT
    TCCATCTGCA GCTCCCAGGG CATCTCGCAG ACAGTCAGTG ACCTCTCTGC GGATCCGTTG
    CCTGCAGGCC TGGAGCTGCC CATCCCTGCA GGCTTGTTGG AGCCCCATGT GGTGTCCAGC
    CAGGAGAGCC TGGACATTTC CCTGTGTAGC ACCGGCAGCC TGGGCAGCCT GGGGAGCCTG
    GGCGAGCCAC TGGACAATGC AGAAACGGCC TCAGTGTCAG ACATGGGCTC CATGTACACA
    GTCACGTCCC TGGACAACCA GCCCCTTGCA GCGCGCCCCA TCAAAGGCTT CGCGGTCGTG
    GAGATAAGGT CCCGAGCCAA AATTGAGAAA ATTCGAGCAA GTTTATTTAA CAACAATGAC
    TTGATAGGTT TGTCAAGCTT GGATGGTGAG GATGAATTGA TGGAAATGTC AACGGAAGAG
    ATCCTCACGG TGTCTGTAGT GAATCAGAGT CTATTCGATA CGCAGGGGAG CCCAGGGTTA
    GAAGATTATT TCAATGATAA GTCAATTAAA GGTGAGAAGC TGGTGCCTGG GGCCAGAGAG
    GTGCTGACGG AGATATTTAA GAGCTGTGCC CACTCGGAGC AGATGCTGAG CCTGACGCCA
    GCAAAGCCCA TCAAAGTGTC GGACATTTAT CTTAGCAAAG AGCAGATCAA CTCCCAGACA
    CCGGGCAACC TCCTCCACCT TTTCTTCACC AATGTCCGGC CCCCCAAAAA GGTGCTAGAG
    GACCAGCTCA CCCAGATCCT GAGGAAGTAC GGTGTGCCGA AGCCCAAGTT TGACAAGAGC
    AAGTACAGCA AGGCGGGGAA GGAGCAGCAC CCCGGGAAGG TGGTGAGCAC CAAGCGGCCC
    ATCACCAAGC CGCCCACCAA GGACAAGGCA GTGCTCAACA GCGTCAGCAG GACTGCCTTG
    AGTGAGAAGA AGCCCACCGT GAAGCCCAAG TCACCTGAAA AGAGCAAGCC AGATGAAAAG
    GACCCTGAAA AGTCACCCAC CAAGAAACAA GACGTCCCAG AGGAGAAGTA CCTGACCTTA
    GAAGGATTTC ACAAGTTTGT GATCGACCGA GCCAAGCAAG ACATCCGCAG CGTCTGGAGA
    GCGATTTTAT CCTGTGGCTA CGATCTTCAT TTTGAGAGGT GTGCCTGCAT CGACGTCCGG
    CATGCGCACA AGGCCTCCAG GAAGTGGACC CTGGAGATGG ATGTGGCGCT CGTGCAGTAC
    ATCAACCGGC TGTGCCGCCA CCTGGCCATC ACGCCCGCGC GTCTGCATCC CCACGAGGTG
    TATCTGGACC CTGCAGACGC TGCTGACCCC AGAGTGGCCT GTCTCTTGAA CGTGCCCATC
    GAGAGCCTGC GCCTGCGCTT CGCTCTGCTG CAGTCCCTCA ACACCACGCT CGAGACCTTC
    TTCCTGCCGC TGGTTGAGCT GCGCCAGACT CCCATGTACA CGCACAGCAT CGCCGCCCTG
    CTCAAGGAGG CCAAAGGGCT GATCTTTTAT GACACAAAGG TGACCGTCAT GAATCGGGTG
    CTAAATGCCA CGGTGCAGAG GACGGCTGAC CATGCAGCAC CAGAAATCAC TCTGGACCCG
    CTGGAGATCG TGGGAGGGGA GATCAGAGCC TCTGAGAACT CCTACTTCTG CCAGGCAGCC
    AGGCAGCTGG CCTCAGTGCC CTCCTCCCAG CTTTGCGTCA AACTGGCGAG TGGCGGCGAC
    CCTACCTATG CCTTCAACAT CCGCTTCACG GGAGAGGAGG TCCATGGCAC CAGTGGCTCC
    TTCCGCCACT TCCTGTGGCA GGTGTGCAGA GAGCTGCAGA GCTCCTCGCT CTCCCTCCTC
    CTGCTGTGCC CCAGCTCCGC CGTCAACAAG AACAAGGGCA AGTACATCCT GACCCCCAGC
    CCCATCACCT ATGGGGAGGA GCAGCTGCTG CACTTCCTGG GCCAGTTGCT AGGGATCGCC
    ATCCGAGCAG ATGTCCCCCT CCCCCTGGAC CTCCTGCCCT CCTTCTGGAA GACACTGGTG
    GGCGAGCCCC TGGACCCTGA CCAAGACCTG CAGGAAGCAG ACGTACTCAC CTACAATTAC
    GTCAAGAAAT TTGAGAGTAT CAACGACGAG ACGGAGTTGG AGGCCCTGTG TGCTGAGATC
    GCCTCCCAGC ACCTGGCCAC TGAGAGCCCC GAGGGGCCCA ACAAGCCCTG CTGCCGGTTC
    ACCTACCTGA CCATGACCGG CGAGGAGGTG GAGCTGTGCA GCCGTGGCCG GCACATCCCC
    GTGGCGTGGG AGAACAAGGA CATCTATGCG GCCGCCATCC GGAGCCTGCG GCTGCGTGAG
    CTACAGAACG TGGAGTGTGT GACGGCCGTG CGCGCGGGCC TTGGGGCCAT CATCCCCCTG
    CAGCTGCTCA CCATGCTCAG CCCACTGGAG ATGGAGCTGC GCACCTGCGG CCTCCCGTAC
    ATCAACCTCG AGTTCCTGAA GGCCCACACC ATGTACCAGG TGGGGCTGAT GGAGACCGAC
    CAGCACATCG AGTTCTTCTG GGGGGCACTG GAGATGTTTG CCCAGGAGGA GCTGTGCAAG
    TTCATCAAGT TTGCCTGCAA CCAGGAGCGC ATCCCGTTCA CCTGCCCCTG CAAAGACGGG
    GGTCCTGACA CGGCCCATGT GCCCCCATAC CCCATGAAGA TTGCCCCCCC AGATGGCACA
    GCAGGTTCCC CAGATTCTCG CTACATCCGA GTGGAGACCT GTATGTTCAT GATCAAGCTT
    CCCCAGTACT CCTCGCTGGA AATCATGCTG GAGAAACTAC GTTGTGCCAT TCACTACCGC
    GAAGACCCTC TGAGTGGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52731
    Clone ID Related Accession (Same CDS sequence) XM_010814059.2 , XM_015467164.1
    Accession Version XM_010814059.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814059.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TTGGTGATAT ACGCGGGGCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTGGTGAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGAA GTGGCCGTGA ACACAACACT CCCGATAAGG CTATGTGGGG CTTTGCTTGC
    ACAGTTCGCG CTCAGGAGTC TTCGGAGGAT GTGTCAGGAG GCTTACCCTT TCTGGTAGAC
    CTGGCTTTAG GTCTATCTGT GTTAGCTTGT TCCATGTTAA GAATCCTATA CAACGGACCA
    GAAATTACCA AAGAAGAAGA AACCTGTCAG GAGCTGTTGC GGTCCAAACT TTTACAAAGG
    TGCCAGTGGC AGGTAGAGGC CAACGGTGTG ATCTCCCCTG CCCTTACTCC AAGCCCATCT
    CCACTGCCTC TGACCATCGA GGAAGACAGA GAGTTCACTT ACCCGTCTGA TGTCCTCGTG
    CCTCCTGTTG GAAACTACTT TGACCTGCCC CGGATCAGGC TGCCTCCAGG AATCATGATA
    AAGCTCAGGG AAATTTCTGG GCGTGCTAGA CCACAGTTTA GACCAAGTAT AAAAGAAGTA
    ATTCAGCCAG ATGTGATGGA GGAAATGGTG GTATCATGTG TTATTAAGCA CTTGAACTTA
    GTTGATGCAC TGCAGTCCCT AATAAATTTC CAGTATCAAG AAGAACATGC TGAGGAATAT
    GATTTACTGT GTAAAATCAT GGGAGAGACA TTTAAGAAAC TCAATGCCAT GGAGAGACAG
    CTGCAGAGTG TTGCAGAATT GGAACAGAAA TGGCAGAGTG AAGTTGATGA TGCCATGCAG
    GGGAAACTGG AGAACAACAT GCCTTTCTTC TATGATTACC ATTTCAATGA GAACAAAATG
    AAAGAACTAG AACTTTTGTG TTCAATGAAG GAAGTCTCCT TTGATGGAAA TGATCTTGAA
    AACATGGTGC TATCTCTGAG GGAGAAGTTC CTACAAGAAG TGAATTCTCT TATTCAGAAA
    CCCTCGCACC CACTGGCTAA AACGAAGACG TTAGTGAAGA GTCTAATGAA CCGAGCTGAG
    CTGTTGCTGC ACGTCACCAT TGCAGCCCAG TCTGGCCTCA CGAGAAGCAT CTCAGGGACC
    CCCGCGGAAA CACCGGCTTG TAAATCAGCC TCTGAAACAA AGGTGATCTC TCATGCTGTG
    CGTCAGCCTG TTTTCCTTCG CAGCATGTCG GCTCCCTCTG ACCTGGAAAT GATCGGTAAT
    GAAGATTTGG AATTTACTAG AGCAAATCAG AGGCGGCGCC ACGTGACCAG CCACCGCAGC
    AGCTCCTTCA CACTCCTGCA GTCATTGGCC ATAGAGGACA GCAGGGACAA GCCCACCTAC
    AGCGTCCTGC TGGGGCAGCT GTTTGCTTTC ATCGGCACCA ACCCTGACCA AGCTGTGTCC
    AGCAGCAGCT TTCTTCTGGC CGCACAGACG AGATGGCGGC GGGGAAACAC CCGCAAGCAG
    GCGTTGGTGC ACATGCGGGA GCTGCTGACG GCTGCCGTGC GAGTCGGGGG AGTGACACAC
    CTTGTGGGTC CAGTGACAAT GGTCCTGCAG GGAGGACCCA GAATTGAAGA ACTGACCTGT
    GGTGGGATGG TCGAGCAGGT CCAGGAAGCC TTCGGTGAGA CCATGACCTC TGTGGTGTCT
    TTGTGCGCAC GTTACCCTAT CGCATGTGCA AATAGCATCG GACTCTTGTG CACTATACCT
    TATACCAGGA GTGAAGAGAA GTGCCTGGTG CGGAGTGGCC TTGTCCAGCT CATGGATCGA
    CTATGCAGCT TGAGCAGTCA GACTGAGTCC AGCTCCAGTG AGAAACAAAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCCTG GGCAGCCTTC CAGGTGCTTG CCAACCGCTG CGTTGAATGG
    GAAAAAGAAG AAGGCGGCTC CACAGAGGCT GTGCACTCAG GTCTGGCCCG TCAGGTGTCC
    AGCCTTCTCA CGAATCACCT TGCCCGAGCG ACCGAGTGCT GTGGCAACCA GGCTGCCGGG
    AACGATGCCC TCCAGGACGT CCTGAGTCTC CTCAATGATC TCTCGAGGAG TCATATAGGT
    AAAGCCATCC TGAGCCAGCC AGCTTGTGTG TCCAAACTCC TCTCCCTGCT ACTTGACCAA
    CGCCCATCCC CAAAGCTGGT TCTTATCATT CTTCAGCTGT GCCGGGCGGC ACTCCCGCTG
    ATGAGCGTAG AAGACTGTGG GAACGTGGAG CTCCCACCCT GGAGCTACTC CGTCCCCTCC
    CTAAACAGCG AGCAGGAGGA TCCCAGCGAC CCAGCGTCCA AGATCGCCTC GCTGCTCTTG
    GCAAAGCTGG CAGACTACGT GGTCCCAGGA TGTCAGACAG TCCTTTCTCC AACCGCTTCC
    GAACCTGACA CCACATTGAC AAAAACCAGC CCCAAGAATT CCTTAAAAGG CGATAAAGAT
    CCTGGAGAAG AGAGTGAAGC AGTGGATGGC AAGCTCTCTA TCTTCATCCA CAAGCGGGAA
    GACCAGTCGT CCCATGAAGT TCTGCAGCCA TTACTAAGTA GCTCAGAAGG ACGGCCCTTC
    CGACTTGGTA CTGGTGCCAA CATGGAGAAA GTTGTGAAAA TGGACCGAGA CATGACGAAG
    GGTGGCTGCT GTGAGGTGAT TACGGAAGAG GCGGCAGCTG CTCTGCGAAA AGCGACCAAG
    TGGGCACAGT CAGGCCTCAT TGTCAGTGTT GGGCCACCCG TGGAGTCCCT CAACCCAGAG
    GCCGTGAGCG GGCTGTCCAC AGGGGACAAG AAGAAGACTG CCCAAACCTC CATTTGCCGA
    GAGAGAAACT CTGAACTCGC CAGGACCGAC CCCGTCCGAC CTTTCATCAG TGGGCACGTG
    GCAAACAGCA TGGCTGCTGA GGTGATCGCC TTGCTTCATA GCTTGCTGAT GGCACCTGAG
    TCAAATGCTG CTCAGATATG GACCACGACA GCAGAGAAGG TTCTGTCTCG TGCACTGATG
    TACATTCCAC AGTTGGGGAA ATATGCAGAA AGCATTCTGG AAAATGGCAG CAGCAGTGGC
    AGAAAGCTTG CCAAACTTCA GAGAATTGCC CGCCAGGCTG TGGCTGCACT CTGTGCACTT
    GGAGGCTTCA AAGAGACAAT CAAGATCGGG TCTGAGGTCC AGGTTTTAGG TAGAGGAATA
    TCAGGAAGCA TCGGAGTGGT GGCTTCTATC AACGAACAGG AAGGTATAGC TACGGTCAGA
    TTCCCACCCA TAGACTGTAG AAAGACTTCG CAAGCATCAG ACACACTGAC TATTCCATTG
    TCTAGACTTT GTGTTCCAAG ATCAGAGGCA TTGCCTCTTC ATAAACTGTC CATTACTGAG
    AAGGTAGTAC AGGCAGTCCA GTCCATGTTG CTTCCTCAGG AGGGAAGTCT CTCTATTCAT
    ACCTCACTTC CTGCAGCAGG AGATGGGTCA GCTCCTGTGA TGGCAGTCGT TCGGCTCCTG
    GCTGAAATAA GAACAAGAGC ATGCCTGGTC ATGGCCCAGC TTTTAGAAGA CAGCTTATTT
    TGTGAAGAAT TCATACAGCA GTGTCCAGCA GCTGTTGAAG TTCTTAACCT AGTAGCCCAG
    GAATGCAGTG CTGGAGAGCG GCTGGCAGTT GTGGAAGTGC AGTGTGAACG GCTGAGGATG
    CTCTACCGGG ACTGTGCTCG GCCCCCGCCG CCTCCGCTGC AGGCTGACCG GAGACAGCCC
    AAAGAGATTA CGTGGAGCCC CTCACGAGTG TTTCCACCTG TCCGAGCCTG CATGTTCTCC
    TCGCACCTCA CTTCTGTCAC CTTCCTAGCA GACCCCAGTG CAGGGGGCGG TCTGCCCAGG
    GGCACATTTA TCTACGCCAC CTCACCACTG CCCGTTCAGG CCCCGTCTTT CTACTGGGAA
    ATTGAAATTG TTTCCTATGG AGATACAGAT GATGATACTG GACCAATAGT TTCCTTTGGC
    TTTGCTACAG AAGCAGAGAA AAGAGATGGG GCATGGACTA ATCCAGTGGG CACCTGTCTT
    TTCCATAACA ATGGCCGAGC TGTGCATTAC AATGGCTCCA GTTTATTACA GTGGAAGAGC
    GTTCGATTAG ACGTAACTCT CTCTCCTGGT GACGTGGCAG GGATTGGCTG GGAGAGAACC
    GAGGGGACGC CTCCTCCACC CGGGCAGCCT GCCAAGGGCC GTGTGTACTT CACATACTGT
    GGGCAGCGCC TCTCACCGTA TCTCGAAGAT GTCTCGGGTG GGATGTGGCC GGTGGTTCAT
    ATTCAGAAAA AGAACACCAA GACTCGCGCT AATTTTGGCT CCCGCCCATT TGCCTACGCT
    GAAGGGCAAG CCCACCGCAA TGCTGCCGAC CTGTGCACTG ACCTGGCCGA GGAAATTAGC
    GCTAACTTCG AGGCCCTGCC CTTTGCCATG GCCTCTGACA GTGATAACGA TGCTGGCACC
    AGTATTGCGT CAGACCCGGG CACTCATGGG CCACCATGCC GAATTGCTGC CGTAGCCACT
    GCTCAGCAAC AATATGATAG TGATACTTCC TGTCATTATA AAGTGGAGCT CAGCTATGAG
    AATTTCATCA CCTCTGGGCC AGACCCTCAC CCACCCCCCA TTGCAGATGA CGAAAGTGAT
    GATGATGATG ACGATGACAT CCCACAGGAG GATCACTATG CCCTGCTGGT GAAAGCCTGG
    GAGACCAAGG TTTTTCCTAC CATCCGAAGA CGGTTCCGCA ACGAAGCAGA GAGGAAATCA
    GGACTGGACC AGATAAAGGG TGCTTTACAA CTAGGCATGG TGGATATTGC CCGGCAGACA
    GTTGAATTCC TCTACGAGGA GAATGGTGGC ATCCCAAGAG ACCTTTATCT TCCCACCATT
    GAAGACATTA AAGATGAAGC GAACAAGTTC ACAATTGATA AAGTTCGAAA AGGTCTCACG
    GTAGTAACAC GCTCTCCAGA CAGCAATAAC GTAGCCAGCA GCACTGTTGG AACTGCCCTG
    CCAAAATTTG CTATCCGAGG GATGCTGAAA ACCTTTGGGC TTCATGGAGT TGTCTTAGAT
    GTTGATTCAG TTAATGAACT AGTTCAGGTA GAAACGTACC TCCGAAGTGA AGGTGTGCTG
    GTCCGATACT GGTACCCTAT TGATATGTTG GAAAGGCCCC CCGCAGGCTA CAGAAGGACT
    GCCACCAATG GGCTCGTCAC ACTGGACAAT ACCAACCTTC AAATTCACAG GGAGCTGCTG
    CGCTGCGAGG CCGCGCTGGC CAGGCTGTAC TGCCGCATGG CCCTGCTCAA CATCTTCGCC
    CCCAAGCTGC CTCACCTGTT TACCCGCCTC TTCCACATCC CCGCCATCCG GGACATTACC
    CTGGAGCACC TGCAGCTGCT GTCCAATCAG CTCCTCGCTC CTCCCCTCCC AGATGGCACC
    ATCAGCTCCA GCTCCATCCT CCTGGCGCAG TCCCTGCAGC ACTGTGTCCA TTCCCAGAAC
    TGTTCGGCCA CAGACCTCTT CTACCAGGGC AGCTCCCAGA CGGTGCGAGA GTGGCTCAAC
    GTGGCCATCA CGCGGACCCT GCACCAGGGC GAGGAAAGCC TTCTGGAGCT GACCAAACAG
    ATCTGCTCTT TCCTGCAGAC AGCACCAGAA CAGTTCCCCT CCGAAGAGTT TCCAATTTCC
    GAGTCCAAAG TCAACATGGA CGTCAATTTC CCCGGGGCAG CTTTCGTCGT CGTATCTTGC
    AAAGAAAGTC AGTCTGGATT CCGCAAAGAT TCCTCTCTGT ACAAGGCACC ATGGGCGCGT
    GTGCTGGTGT ACGGGCTAGG CCACAAAGTG AAGCGAAATG GCCAGCTGAA CCTCATTGAG
    GCCGTCTGTT ACCCGCGGGA TGCGTCTCCG GCCAACACAG GGCTCACACC ACCTCCTACC
    ACCAACCAGT ACCCTTCTGT GATCCTCTCC ACAGACAAAG TCCACATCAA ACTGGGGGTA
    TCTCCCCCAC CCGGAGCCGT GCTGGTGTTA CACTCCCTGC CTCTCGAGTT CCCCCTGGCG
    ATGGCCTTCA CCGAGCAGCT ACTGTCCTGG AAATCAGAGG ATGGCGAAGG TAGAGCTGAA
    GATGAGCCTG ACACCATCCC GACGTCCGTC CTCCTGCAAG TGGTGGAGCT GCTTGGCAAC
    TTCCTCTGGA CCACAGACAT GGCGGCCTGC GTGAAGGAGC TGGTTTTCCA CCTCCTGGCA
    GAGCTCCTGC GCACGGTGCA CAGCCTAGAG CAGAGGAAGC ACCCAGCCGG CCTGTCCTCA
    TCCATCGCCC TCCAGCTGAA CCCCTGCCTG GCCATGCTGA TGGCCCTGCA GTCGGAGCTT
    CACAAGCTGT ACGACGAGGA GACGCAGAGC TGGGTCTCGG GCAGTGCATG CGGAGGCTCA
    GGAGCCACGG CGGCTGCTGA CCAGGGCCGG TTCTCGACGT ATTTCCACGC ACTCATGGAG
    GGCTGCCTGG CGGTGGCCGA GGTGACCCTG CCCACCAACA TGAGTGTCAC CGCCAGCGGG
    GTGACGTCAG CAACTGCCCC AAACCTCAGC GACTCGTCCT CCTCCTCATC GTCCTCCCCA
    GGACAGACTC CACAGAGCCC CAGCCTCCTG TCCAAGAGGA AGAAAGTCAA GATGAAGCGG
    GAAAAGGCCT CCTCCTCTGG GAAGCGCCAG TCCTCACGCT CTGTGGACTC GGACCCCGCC
    GTGCTGAGCA TCGGGGGCAG CAAGCCCGAG GACATGCTGT GGTTCCATCG TGCTCTGACG
    TTGCTCATCA TCCTCCGCCA CCTCACCAGG AAGGACCCAC AGGGGCTAGG CGTGACAAGC
    GACGCCATCG CCGACGCCTG CCAGGCCCTG GTGGGCCCCA CCGCCCACAG CCGCTTGCTG
    GTGATCTCTG GGATCCCCAC CCACCTGGAC GAGGGCATCG TCAGGGGTGC CATCCGCAAG
    GCCTGCAATG CCCATGGCGG GGTGTTCAAG GATGAGATCT ACATCCCCCT GCAGGACGAA
    GACCCCAAGA AGCCAAAAGA TAAGGCCGAG GGGGGTGATG GGAAAGCCGA GCCGGAAAAG
    ACCCTCGGCT TCCCCAGCAC GGACAGCTTG GAGGTCAGCA CATCCAGCAG CCTGACCCCC
    GCTATGAGCG TCAGCGCATC CGCGTCCACC AGCCAGGCTT CCATCTGCAG CTCCCAGGGC
    ATCTCGCAGA CAGTCAGTGA CCTCTCTGCG GATCCGTTGC CTGCAGGCCT GGAGCTGCCC
    ATCCCTGCAG GCTTGTTGGA GCCCCATGTG GTGTCCAGCC AGGAGAGCCT GGACATTTCC
    CTGTGTAGCA CCGGCAGCCT GGGCAGCCTG GGGAGCCTGG GCGAGCCACT GGACAATGCA
    GAAACGGCCT CAGTGTCAGA CATGGGCTCC ATGTACACAG TCACGTCCCT GGACAACCAG
    CCCCTTGCAG CGCGCCCCAT CAAAGGCTTC GCGGTCGTGG AGATAAGGTC CCGAGCCAAA
    ATTGAGAAAA TTCGAGCAAG TTTATTTAAC AACAATGACT TGATAGGTTT GTCAAGCTTG
    GATGGTGAGG ATGAATTGAT GGAAATGTCA ACGGAAGAGA TCCTCACGGT GTCTGTAGTG
    AATCAGAGTC TATTCGATAC GCAGGGGAGC CCAGGGTTAG AAGATTATTT CAATGATAAG
    TCAATTAAAG GTGAGAAGCT GGTGCCTGGG GCCAGAGAGG TGCTGACGGA GATATTTAAG
    AGCTGTGCCC ACTCGGAGCA GATGCTGAGC CTGACGCCAG CAAAGCCCAT CAAAGTGTCG
    GACATTTATC TTAGCAAAGA GCAGATCAAC TCCCAGACAC CGGGCAACCT CCTCCACCTT
    TTCTTCACCA ATGTCCGGCC CCCCAAAAAG GTGCTAGAGG ACCAGCTCAC CCAGATCCTG
    AGGAAGTACG GTGTGCCGAA GCCCAAGTTT GACAAGAGCA AGTACAGCAA GGCGGGGAAG
    GAGCAGCACC CCGGGAAGGT GGTGAGCACC AAGCGGCCCA TCACCAAGCC GCCCACCAAG
    GACAAGGCAG TGCTCAACAG CGTCAGCAGG ACTGCCTTGA GTGAGAAGAA GCCCACCGTG
    AAGCCCAAGT CACCTGAAAA GAGCAAGCCA GATGAAAAGG ACCCTGAAAA GTCACCCACC
    AAGAAACAAG ACGTCCCAGA GGAGAAGTAC CTGACCTTAG AAGGATTTCA CAAGTTTGTG
    ATCGACCGAG CCAAGCAAGA CATCCGCAGC GTCTGGAGAG CGATTTTATC CTGTGGCTAC
    GATCTTCATT TTGAGAGGTG TGCCTGCATC GACGTCCGGC ATGCGCACAA GGCCTCCAGG
    AAGTGGACCC TGGAGATGGA TGTGGCGCTC GTGCAGTACA TCAACCGGCT GTGCCGCCAC
    CTGGCCATCA CGCCCGCGCG TCTGCATCCC CACGAGGTGT ATCTGGACCC TGCAGACGCT
    GCTGACCCCA GAGTGGCCTG TCTCTTGAAC GTGCCCATCG AGAGCCTGCG CCTGCGCTTC
    GCTCTGCTGC AGTCCCTCAA CACCACGCTC GAGACCTTCT TCCTGCCGCT GGTTGAGCTG
    CGCCAGACTC CCATGTACAC GCACAGCATC GCCGCCCTGC TCAAGGAGGC CAAAGGGCTG
    ATCTTTTATG ACACAAAGGT GACCGTCATG AATCGGGTGC TAAATGCCAC GGTGCAGAGG
    ACGGCTGACC ATGCAGCACC AGAAATCACT CTGGACCCGC TGGAGATCGT GGGAGGGGAG
    ATCAGAGCCT CTGAGAACTC CTACTTCTGC CAGGCAGCCA GGCAGCTGGC CTCAGTGCCC
    TCCTCCCAGC TTTGCGTCAA ACTGGCGAGT GGCGGCGACC CTACCTATGC CTTCAACATC
    CGCTTCACGG GAGAGGAGGT CCATGGCACC AGTGGCTCCT TCCGCCACTT CCTGTGGCAG
    GTGTGCAGAG AGCTGCAGAG CTCCTCGCTC TCCCTCCTCC TGCTGTGCCC CAGCTCCGCC
    GTCAACAAGA ACAAGGGCAA GTACATCCTG ACCCCCAGCC CCATCACCTA TGGGGAGGAG
    CAGCTGCTGC ACTTCCTGGG CCAGTTGCTA GGGATCGCCA TCCGAGCAGA TGTCCCCCTC
    CCCCTGGACC TCCTGCCCTC CTTCTGGAAG ACACTGGTGG GCGAGCCCCT GGACCCTGAC
    CAAGACCTGC AGGAAGCAGA CGTACTCACC TACAATTACG TCAAGAAATT TGAGAGTATC
    AACGACGAGA CGGAGTTGGA GGCCCTGTGT GCTGAGATCG CCTCCCAGCA CCTGGCCACT
    GAGAGCCCCG AGGGGCCCAA CAAGCCCTGC TGCCGGTTCA CCTACCTGAC CATGACCGGC
    GAGGAGGTGG AGCTGTGCAG CCGTGGCCGG CACATCCCCG TGGCGTGGGA GAACAAGGAC
    ATCTATGCGG CCGCCATCCG GAGCCTGCGG CTGCGTGAGC TACAGAACGT GGAGTGTGTG
    ACGGCCGTGC GCGCGGGCCT TGGGGCCATC ATCCCCCTGC AGCTGCTCAC CATGCTCAGC
    CCACTGGAGA TGGAGCTGCG CACCTGCGGC CTCCCGTACA TCAACCTCGA GTTCCTGAAG
    GCCCACACCA TGTACCAGGT GGGGCTGATG GAGACCGACC AGCACATCGA GTTCTTCTGG
    GGGGCACTGG AGATGTTTGC CCAGGAGGAG CTGTGCAAGT TCATCAAGTT TGCCTGCAAC
    CAGGAGCGCA TCCCGTTCAC CTGCCCCTGC AAAGACGGGG GTCCTGACAC GGCCCATGTG
    CCCCCATACC CCATGAAGAT TGCCCCCCCA GATGGCACAG CAGGTTCCCC AGATTCTCGC
    TACATCCGAG TGGAGACCTG TATGTTCATG ATCAAGCTTC CCCAGTACTC CTCGCTGGAA
    ATCATGCTGG AGAAACTACG TTGTGCCATT CACTACCGCG AAGACCCTCT GAGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812361.1
    CDS1025..12724
    Translation

    Target ORF information:

    RefSeq Version XM_010814059.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814059.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TTGGTGATAT ACGCGGGGCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTGGTGAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGAA GTGGCCGTGA ACACAACACT CCCGATAAGG CTATGTGGGG CTTTGCTTGC
    ACAGTTCGCG CTCAGGAGTC TTCGGAGGAT GTGTCAGGAG GCTTACCCTT TCTGGTAGAC
    CTGGCTTTAG GTCTATCTGT GTTAGCTTGT TCCATGTTAA GAATCCTATA CAACGGACCA
    GAAATTACCA AAGAAGAAGA AACCTGTCAG GAGCTGTTGC GGTCCAAACT TTTACAAAGG
    TGCCAGTGGC AGGTAGAGGC CAACGGTGTG ATCTCCCCTG CCCTTACTCC AAGCCCATCT
    CCACTGCCTC TGACCATCGA GGAAGACAGA GAGTTCACTT ACCCGTCTGA TGTCCTCGTG
    CCTCCTGTTG GAAACTACTT TGACCTGCCC CGGATCAGGC TGCCTCCAGG AATCATGATA
    AAGCTCAGGG AAATTTCTGG GCGTGCTAGA CCACAGTTTA GACCAAGTAT AAAAGAAGTA
    ATTCAGCCAG ATGTGATGGA GGAAATGGTG GTATCATGTG TTATTAAGCA CTTGAACTTA
    GTTGATGCAC TGCAGTCCCT AATAAATTTC CAGTATCAAG AAGAACATGC TGAGGAATAT
    GATTTACTGT GTAAAATCAT GGGAGAGACA TTTAAGAAAC TCAATGCCAT GGAGAGACAG
    CTGCAGAGTG TTGCAGAATT GGAACAGAAA TGGCAGAGTG AAGTTGATGA TGCCATGCAG
    GGGAAACTGG AGAACAACAT GCCTTTCTTC TATGATTACC ATTTCAATGA GAACAAAATG
    AAAGAACTAG AACTTTTGTG TTCAATGAAG GAAGTCTCCT TTGATGGAAA TGATCTTGAA
    AACATGGTGC TATCTCTGAG GGAGAAGTTC CTACAAGAAG TGAATTCTCT TATTCAGAAA
    CCCTCGCACC CACTGGCTAA AACGAAGACG TTAGTGAAGA GTCTAATGAA CCGAGCTGAG
    CTGTTGCTGC ACGTCACCAT TGCAGCCCAG TCTGGCCTCA CGAGAAGCAT CTCAGGGACC
    CCCGCGGAAA CACCGGCTTG TAAATCAGCC TCTGAAACAA AGGTGATCTC TCATGCTGTG
    CGTCAGCCTG TTTTCCTTCG CAGCATGTCG GCTCCCTCTG ACCTGGAAAT GATCGGTAAT
    GAAGATTTGG AATTTACTAG AGCAAATCAG AGGCGGCGCC ACGTGACCAG CCACCGCAGC
    AGCTCCTTCA CACTCCTGCA GTCATTGGCC ATAGAGGACA GCAGGGACAA GCCCACCTAC
    AGCGTCCTGC TGGGGCAGCT GTTTGCTTTC ATCGGCACCA ACCCTGACCA AGCTGTGTCC
    AGCAGCAGCT TTCTTCTGGC CGCACAGACG AGATGGCGGC GGGGAAACAC CCGCAAGCAG
    GCGTTGGTGC ACATGCGGGA GCTGCTGACG GCTGCCGTGC GAGTCGGGGG AGTGACACAC
    CTTGTGGGTC CAGTGACAAT GGTCCTGCAG GGAGGACCCA GAATTGAAGA ACTGACCTGT
    GGTGGGATGG TCGAGCAGGT CCAGGAAGCC TTCGGTGAGA CCATGACCTC TGTGGTGTCT
    TTGTGCGCAC GTTACCCTAT CGCATGTGCA AATAGCATCG GACTCTTGTG CACTATACCT
    TATACCAGGA GTGAAGAGAA GTGCCTGGTG CGGAGTGGCC TTGTCCAGCT CATGGATCGA
    CTATGCAGCT TGAGCAGTCA GACTGAGTCC AGCTCCAGTG AGAAACAAAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCCTG GGCAGCCTTC CAGGTGCTTG CCAACCGCTG CGTTGAATGG
    GAAAAAGAAG AAGGCGGCTC CACAGAGGCT GTGCACTCAG GTCTGGCCCG TCAGGTGTCC
    AGCCTTCTCA CGAATCACCT TGCCCGAGCG ACCGAGTGCT GTGGCAACCA GGCTGCCGGG
    AACGATGCCC TCCAGGACGT CCTGAGTCTC CTCAATGATC TCTCGAGGAG TCATATAGGT
    AAAGCCATCC TGAGCCAGCC AGCTTGTGTG TCCAAACTCC TCTCCCTGCT ACTTGACCAA
    CGCCCATCCC CAAAGCTGGT TCTTATCATT CTTCAGCTGT GCCGGGCGGC ACTCCCGCTG
    ATGAGCGTAG AAGACTGTGG GAACGTGGAG CTCCCACCCT GGAGCTACTC CGTCCCCTCC
    CTAAACAGCG AGCAGGAGGA TCCCAGCGAC CCAGCGTCCA AGATCGCCTC GCTGCTCTTG
    GCAAAGCTGG CAGACTACGT GGTCCCAGGA TGTCAGACAG TCCTTTCTCC AACCGCTTCC
    GAACCTGACA CCACATTGAC AAAAACCAGC CCCAAGAATT CCTTAAAAGG CGATAAAGAT
    CCTGGAGAAG AGAGTGAAGC AGTGGATGGC AAGCTCTCTA TCTTCATCCA CAAGCGGGAA
    GACCAGTCGT CCCATGAAGT TCTGCAGCCA TTACTAAGTA GCTCAGAAGG ACGGCCCTTC
    CGACTTGGTA CTGGTGCCAA CATGGAGAAA GTTGTGAAAA TGGACCGAGA CATGACGAAG
    GGTGGCTGCT GTGAGGTGAT TACGGAAGAG GCGGCAGCTG CTCTGCGAAA AGCGACCAAG
    TGGGCACAGT CAGGCCTCAT TGTCAGTGTT GGGCCACCCG TGGAGTCCCT CAACCCAGAG
    GCCGTGAGCG GGCTGTCCAC AGGGGACAAG AAGAAGACTG CCCAAACCTC CATTTGCCGA
    GAGAGAAACT CTGAACTCGC CAGGACCGAC CCCGTCCGAC CTTTCATCAG TGGGCACGTG
    GCAAACAGCA TGGCTGCTGA GGTGATCGCC TTGCTTCATA GCTTGCTGAT GGCACCTGAG
    TCAAATGCTG CTCAGATATG GACCACGACA GCAGAGAAGG TTCTGTCTCG TGCACTGATG
    TACATTCCAC AGTTGGGGAA ATATGCAGAA AGCATTCTGG AAAATGGCAG CAGCAGTGGC
    AGAAAGCTTG CCAAACTTCA GAGAATTGCC CGCCAGGCTG TGGCTGCACT CTGTGCACTT
    GGAGGCTTCA AAGAGACAAT CAAGATCGGG TCTGAGGTCC AGGTTTTAGG TAGAGGAATA
    TCAGGAAGCA TCGGAGTGGT GGCTTCTATC AACGAACAGG AAGGTATAGC TACGGTCAGA
    TTCCCACCCA TAGACTGTAG AAAGACTTCG CAAGCATCAG ACACACTGAC TATTCCATTG
    TCTAGACTTT GTGTTCCAAG ATCAGAGGCA TTGCCTCTTC ATAAACTGTC CATTACTGAG
    AAGGTAGTAC AGGCAGTCCA GTCCATGTTG CTTCCTCAGG AGGGAAGTCT CTCTATTCAT
    ACCTCACTTC CTGCAGCAGG AGATGGGTCA GCTCCTGTGA TGGCAGTCGT TCGGCTCCTG
    GCTGAAATAA GAACAAGAGC ATGCCTGGTC ATGGCCCAGC TTTTAGAAGA CAGCTTATTT
    TGTGAAGAAT TCATACAGCA GTGTCCAGCA GCTGTTGAAG TTCTTAACCT AGTAGCCCAG
    GAATGCAGTG CTGGAGAGCG GCTGGCAGTT GTGGAAGTGC AGTGTGAACG GCTGAGGATG
    CTCTACCGGG ACTGTGCTCG GCCCCCGCCG CCTCCGCTGC AGGCTGACCG GAGACAGCCC
    AAAGAGATTA CGTGGAGCCC CTCACGAGTG TTTCCACCTG TCCGAGCCTG CATGTTCTCC
    TCGCACCTCA CTTCTGTCAC CTTCCTAGCA GACCCCAGTG CAGGGGGCGG TCTGCCCAGG
    GGCACATTTA TCTACGCCAC CTCACCACTG CCCGTTCAGG CCCCGTCTTT CTACTGGGAA
    ATTGAAATTG TTTCCTATGG AGATACAGAT GATGATACTG GACCAATAGT TTCCTTTGGC
    TTTGCTACAG AAGCAGAGAA AAGAGATGGG GCATGGACTA ATCCAGTGGG CACCTGTCTT
    TTCCATAACA ATGGCCGAGC TGTGCATTAC AATGGCTCCA GTTTATTACA GTGGAAGAGC
    GTTCGATTAG ACGTAACTCT CTCTCCTGGT GACGTGGCAG GGATTGGCTG GGAGAGAACC
    GAGGGGACGC CTCCTCCACC CGGGCAGCCT GCCAAGGGCC GTGTGTACTT CACATACTGT
    GGGCAGCGCC TCTCACCGTA TCTCGAAGAT GTCTCGGGTG GGATGTGGCC GGTGGTTCAT
    ATTCAGAAAA AGAACACCAA GACTCGCGCT AATTTTGGCT CCCGCCCATT TGCCTACGCT
    GAAGGGCAAG CCCACCGCAA TGCTGCCGAC CTGTGCACTG ACCTGGCCGA GGAAATTAGC
    GCTAACTTCG AGGCCCTGCC CTTTGCCATG GCCTCTGACA GTGATAACGA TGCTGGCACC
    AGTATTGCGT CAGACCCGGG CACTCATGGG CCACCATGCC GAATTGCTGC CGTAGCCACT
    GCTCAGCAAC AATATGATAG TGATACTTCC TGTCATTATA AAGTGGAGCT CAGCTATGAG
    AATTTCATCA CCTCTGGGCC AGACCCTCAC CCACCCCCCA TTGCAGATGA CGAAAGTGAT
    GATGATGATG ACGATGACAT CCCACAGGAG GATCACTATG CCCTGCTGGT GAAAGCCTGG
    GAGACCAAGG TTTTTCCTAC CATCCGAAGA CGGTTCCGCA ACGAAGCAGA GAGGAAATCA
    GGACTGGACC AGATAAAGGG TGCTTTACAA CTAGGCATGG TGGATATTGC CCGGCAGACA
    GTTGAATTCC TCTACGAGGA GAATGGTGGC ATCCCAAGAG ACCTTTATCT TCCCACCATT
    GAAGACATTA AAGATGAAGC GAACAAGTTC ACAATTGATA AAGTTCGAAA AGGTCTCACG
    GTAGTAACAC GCTCTCCAGA CAGCAATAAC GTAGCCAGCA GCACTGTTGG AACTGCCCTG
    CCAAAATTTG CTATCCGAGG GATGCTGAAA ACCTTTGGGC TTCATGGAGT TGTCTTAGAT
    GTTGATTCAG TTAATGAACT AGTTCAGGTA GAAACGTACC TCCGAAGTGA AGGTGTGCTG
    GTCCGATACT GGTACCCTAT TGATATGTTG GAAAGGCCCC CCGCAGGCTA CAGAAGGACT
    GCCACCAATG GGCTCGTCAC ACTGGACAAT ACCAACCTTC AAATTCACAG GGAGCTGCTG
    CGCTGCGAGG CCGCGCTGGC CAGGCTGTAC TGCCGCATGG CCCTGCTCAA CATCTTCGCC
    CCCAAGCTGC CTCACCTGTT TACCCGCCTC TTCCACATCC CCGCCATCCG GGACATTACC
    CTGGAGCACC TGCAGCTGCT GTCCAATCAG CTCCTCGCTC CTCCCCTCCC AGATGGCACC
    ATCAGCTCCA GCTCCATCCT CCTGGCGCAG TCCCTGCAGC ACTGTGTCCA TTCCCAGAAC
    TGTTCGGCCA CAGACCTCTT CTACCAGGGC AGCTCCCAGA CGGTGCGAGA GTGGCTCAAC
    GTGGCCATCA CGCGGACCCT GCACCAGGGC GAGGAAAGCC TTCTGGAGCT GACCAAACAG
    ATCTGCTCTT TCCTGCAGAC AGCACCAGAA CAGTTCCCCT CCGAAGAGTT TCCAATTTCC
    GAGTCCAAAG TCAACATGGA CGTCAATTTC CCCGGGGCAG CTTTCGTCGT CGTATCTTGC
    AAAGAAAGTC AGTCTGGATT CCGCAAAGAT TCCTCTCTGT ACAAGGCACC ATGGGCGCGT
    GTGCTGGTGT ACGGGCTAGG CCACAAAGTG AAGCGAAATG GCCAGCTGAA CCTCATTGAG
    GCCGTCTGTT ACCCGCGGGA TGCGTCTCCG GCCAACACAG GGCTCACACC ACCTCCTACC
    ACCAACCAGT ACCCTTCTGT GATCCTCTCC ACAGACAAAG TCCACATCAA ACTGGGGGTA
    TCTCCCCCAC CCGGAGCCGT GCTGGTGTTA CACTCCCTGC CTCTCGAGTT CCCCCTGGCG
    ATGGCCTTCA CCGAGCAGCT ACTGTCCTGG AAATCAGAGG ATGGCGAAGG TAGAGCTGAA
    GATGAGCCTG ACACCATCCC GACGTCCGTC CTCCTGCAAG TGGTGGAGCT GCTTGGCAAC
    TTCCTCTGGA CCACAGACAT GGCGGCCTGC GTGAAGGAGC TGGTTTTCCA CCTCCTGGCA
    GAGCTCCTGC GCACGGTGCA CAGCCTAGAG CAGAGGAAGC ACCCAGCCGG CCTGTCCTCA
    TCCATCGCCC TCCAGCTGAA CCCCTGCCTG GCCATGCTGA TGGCCCTGCA GTCGGAGCTT
    CACAAGCTGT ACGACGAGGA GACGCAGAGC TGGGTCTCGG GCAGTGCATG CGGAGGCTCA
    GGAGCCACGG CGGCTGCTGA CCAGGGCCGG TTCTCGACGT ATTTCCACGC ACTCATGGAG
    GGCTGCCTGG CGGTGGCCGA GGTGACCCTG CCCACCAACA TGAGTGTCAC CGCCAGCGGG
    GTGACGTCAG CAACTGCCCC AAACCTCAGC GACTCGTCCT CCTCCTCATC GTCCTCCCCA
    GGACAGACTC CACAGAGCCC CAGCCTCCTG TCCAAGAGGA AGAAAGTCAA GATGAAGCGG
    GAAAAGGCCT CCTCCTCTGG GAAGCGCCAG TCCTCACGCT CTGTGGACTC GGACCCCGCC
    GTGCTGAGCA TCGGGGGCAG CAAGCCCGAG GACATGCTGT GGTTCCATCG TGCTCTGACG
    TTGCTCATCA TCCTCCGCCA CCTCACCAGG AAGGACCCAC AGGGGCTAGG CGTGACAAGC
    GACGCCATCG CCGACGCCTG CCAGGCCCTG GTGGGCCCCA CCGCCCACAG CCGCTTGCTG
    GTGATCTCTG GGATCCCCAC CCACCTGGAC GAGGGCATCG TCAGGGGTGC CATCCGCAAG
    GCCTGCAATG CCCATGGCGG GGTGTTCAAG GATGAGATCT ACATCCCCCT GCAGGACGAA
    GACCCCAAGA AGCCAAAAGA TAAGGCCGAG GGGGGTGATG GGAAAGCCGA GCCGGAAAAG
    ACCCTCGGCT TCCCCAGCAC GGACAGCTTG GAGGTCAGCA CATCCAGCAG CCTGACCCCC
    GCTATGAGCG TCAGCGCATC CGCGTCCACC AGCCAGGCTT CCATCTGCAG CTCCCAGGGC
    ATCTCGCAGA CAGTCAGTGA CCTCTCTGCG GATCCGTTGC CTGCAGGCCT GGAGCTGCCC
    ATCCCTGCAG GCTTGTTGGA GCCCCATGTG GTGTCCAGCC AGGAGAGCCT GGACATTTCC
    CTGTGTAGCA CCGGCAGCCT GGGCAGCCTG GGGAGCCTGG GCGAGCCACT GGACAATGCA
    GAAACGGCCT CAGTGTCAGA CATGGGCTCC ATGTACACAG TCACGTCCCT GGACAACCAG
    CCCCTTGCAG CGCGCCCCAT CAAAGGCTTC GCGGTCGTGG AGATAAGGTC CCGAGCCAAA
    ATTGAGAAAA TTCGAGCAAG TTTATTTAAC AACAATGACT TGATAGGTTT GTCAAGCTTG
    GATGGTGAGG ATGAATTGAT GGAAATGTCA ACGGAAGAGA TCCTCACGGT GTCTGTAGTG
    AATCAGAGTC TATTCGATAC GCAGGGGAGC CCAGGGTTAG AAGATTATTT CAATGATAAG
    TCAATTAAAG GTGAGAAGCT GGTGCCTGGG GCCAGAGAGG TGCTGACGGA GATATTTAAG
    AGCTGTGCCC ACTCGGAGCA GATGCTGAGC CTGACGCCAG CAAAGCCCAT CAAAGTGTCG
    GACATTTATC TTAGCAAAGA GCAGATCAAC TCCCAGACAC CGGGCAACCT CCTCCACCTT
    TTCTTCACCA ATGTCCGGCC CCCCAAAAAG GTGCTAGAGG ACCAGCTCAC CCAGATCCTG
    AGGAAGTACG GTGTGCCGAA GCCCAAGTTT GACAAGAGCA AGTACAGCAA GGCGGGGAAG
    GAGCAGCACC CCGGGAAGGT GGTGAGCACC AAGCGGCCCA TCACCAAGCC GCCCACCAAG
    GACAAGGCAG TGCTCAACAG CGTCAGCAGG ACTGCCTTGA GTGAGAAGAA GCCCACCGTG
    AAGCCCAAGT CACCTGAAAA GAGCAAGCCA GATGAAAAGG ACCCTGAAAA GTCACCCACC
    AAGAAACAAG ACGTCCCAGA GGAGAAGTAC CTGACCTTAG AAGGATTTCA CAAGTTTGTG
    ATCGACCGAG CCAAGCAAGA CATCCGCAGC GTCTGGAGAG CGATTTTATC CTGTGGCTAC
    GATCTTCATT TTGAGAGGTG TGCCTGCATC GACGTCCGGC ATGCGCACAA GGCCTCCAGG
    AAGTGGACCC TGGAGATGGA TGTGGCGCTC GTGCAGTACA TCAACCGGCT GTGCCGCCAC
    CTGGCCATCA CGCCCGCGCG TCTGCATCCC CACGAGGTGT ATCTGGACCC TGCAGACGCT
    GCTGACCCCA GAGTGGCCTG TCTCTTGAAC GTGCCCATCG AGAGCCTGCG CCTGCGCTTC
    GCTCTGCTGC AGTCCCTCAA CACCACGCTC GAGACCTTCT TCCTGCCGCT GGTTGAGCTG
    CGCCAGACTC CCATGTACAC GCACAGCATC GCCGCCCTGC TCAAGGAGGC CAAAGGGCTG
    ATCTTTTATG ACACAAAGGT GACCGTCATG AATCGGGTGC TAAATGCCAC GGTGCAGAGG
    ACGGCTGACC ATGCAGCACC AGAAATCACT CTGGACCCGC TGGAGATCGT GGGAGGGGAG
    ATCAGAGCCT CTGAGAACTC CTACTTCTGC CAGGCAGCCA GGCAGCTGGC CTCAGTGCCC
    TCCTCCCAGC TTTGCGTCAA ACTGGCGAGT GGCGGCGACC CTACCTATGC CTTCAACATC
    CGCTTCACGG GAGAGGAGGT CCATGGCACC AGTGGCTCCT TCCGCCACTT CCTGTGGCAG
    GTGTGCAGAG AGCTGCAGAG CTCCTCGCTC TCCCTCCTCC TGCTGTGCCC CAGCTCCGCC
    GTCAACAAGA ACAAGGGCAA GTACATCCTG ACCCCCAGCC CCATCACCTA TGGGGAGGAG
    CAGCTGCTGC ACTTCCTGGG CCAGTTGCTA GGGATCGCCA TCCGAGCAGA TGTCCCCCTC
    CCCCTGGACC TCCTGCCCTC CTTCTGGAAG ACACTGGTGG GCGAGCCCCT GGACCCTGAC
    CAAGACCTGC AGGAAGCAGA CGTACTCACC TACAATTACG TCAAGAAATT TGAGAGTATC
    AACGACGAGA CGGAGTTGGA GGCCCTGTGT GCTGAGATCG CCTCCCAGCA CCTGGCCACT
    GAGAGCCCCG AGGGGCCCAA CAAGCCCTGC TGCCGGTTCA CCTACCTGAC CATGACCGGC
    GAGGAGGTGG AGCTGTGCAG CCGTGGCCGG CACATCCCCG TGGCGTGGGA GAACAAGGAC
    ATCTATGCGG CCGCCATCCG GAGCCTGCGG CTGCGTGAGC TACAGAACGT GGAGTGTGTG
    ACGGCCGTGC GCGCGGGCCT TGGGGCCATC ATCCCCCTGC AGCTGCTCAC CATGCTCAGC
    CCACTGGAGA TGGAGCTGCG CACCTGCGGC CTCCCGTACA TCAACCTCGA GTTCCTGAAG
    GCCCACACCA TGTACCAGGT GGGGCTGATG GAGACCGACC AGCACATCGA GTTCTTCTGG
    GGGGCACTGG AGATGTTTGC CCAGGAGGAG CTGTGCAAGT TCATCAAGTT TGCCTGCAAC
    CAGGAGCGCA TCCCGTTCAC CTGCCCCTGC AAAGACGGGG GTCCTGACAC GGCCCATGTG
    CCCCCATACC CCATGAAGAT TGCCCCCCCA GATGGCACAG CAGGTTCCCC AGATTCTCGC
    TACATCCGAG TGGAGACCTG TATGTTCATG ATCAAGCTTC CCCAGTACTC CTCGCTGGAA
    ATCATGCTGG AGAAACTACG TTGTGCCATT CACTACCGCG AAGACCCTCT GAGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52731
    Clone ID Related Accession (Same CDS sequence) XM_010814059.2 , XM_015467164.1
    Accession Version XM_015467164.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11700bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TTGGTGATAT ACGCGGGGCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTGGTGAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGAA GTGGCCGTGA ACACAACACT CCCGATAAGG CTATGTGGGG CTTTGCTTGC
    ACAGTTCGCG CTCAGGAGTC TTCGGAGGAT GTGTCAGGAG GCTTACCCTT TCTGGTAGAC
    CTGGCTTTAG GTCTATCTGT GTTAGCTTGT TCCATGTTAA GAATCCTATA CAACGGACCA
    GAAATTACCA AAGAAGAAGA AACCTGTCAG GAGCTGTTGC GGTCCAAACT TTTACAAAGG
    TGCCAGTGGC AGGTAGAGGC CAACGGTGTG ATCTCCCCTG CCCTTACTCC AAGCCCATCT
    CCACTGCCTC TGACCATCGA GGAAGACAGA GAGTTCACTT ACCCGTCTGA TGTCCTCGTG
    CCTCCTGTTG GAAACTACTT TGACCTGCCC CGGATCAGGC TGCCTCCAGG AATCATGATA
    AAGCTCAGGG AAATTTCTGG GCGTGCTAGA CCACAGTTTA GACCAAGTAT AAAAGAAGTA
    ATTCAGCCAG ATGTGATGGA GGAAATGGTG GTATCATGTG TTATTAAGCA CTTGAACTTA
    GTTGATGCAC TGCAGTCCCT AATAAATTTC CAGTATCAAG AAGAACATGC TGAGGAATAT
    GATTTACTGT GTAAAATCAT GGGAGAGACA TTTAAGAAAC TCAATGCCAT GGAGAGACAG
    CTGCAGAGTG TTGCAGAATT GGAACAGAAA TGGCAGAGTG AAGTTGATGA TGCCATGCAG
    GGGAAACTGG AGAACAACAT GCCTTTCTTC TATGATTACC ATTTCAATGA GAACAAAATG
    AAAGAACTAG AACTTTTGTG TTCAATGAAG GAAGTCTCCT TTGATGGAAA TGATCTTGAA
    AACATGGTGC TATCTCTGAG GGAGAAGTTC CTACAAGAAG TGAATTCTCT TATTCAGAAA
    CCCTCGCACC CACTGGCTAA AACGAAGACG TTAGTGAAGA GTCTAATGAA CCGAGCTGAG
    CTGTTGCTGC ACGTCACCAT TGCAGCCCAG TCTGGCCTCA CGAGAAGCAT CTCAGGGACC
    CCCGCGGAAA CACCGGCTTG TAAATCAGCC TCTGAAACAA AGGTGATCTC TCATGCTGTG
    CGTCAGCCTG TTTTCCTTCG CAGCATGTCG GCTCCCTCTG ACCTGGAAAT GATCGGTAAT
    GAAGATTTGG AATTTACTAG AGCAAATCAG AGGCGGCGCC ACGTGACCAG CCACCGCAGC
    AGCTCCTTCA CACTCCTGCA GTCATTGGCC ATAGAGGACA GCAGGGACAA GCCCACCTAC
    AGCGTCCTGC TGGGGCAGCT GTTTGCTTTC ATCGGCACCA ACCCTGACCA AGCTGTGTCC
    AGCAGCAGCT TTCTTCTGGC CGCACAGACG AGATGGCGGC GGGGAAACAC CCGCAAGCAG
    GCGTTGGTGC ACATGCGGGA GCTGCTGACG GCTGCCGTGC GAGTCGGGGG AGTGACACAC
    CTTGTGGGTC CAGTGACAAT GGTCCTGCAG GGAGGACCCA GAATTGAAGA ACTGACCTGT
    GGTGGGATGG TCGAGCAGGT CCAGGAAGCC TTCGGTGAGA CCATGACCTC TGTGGTGTCT
    TTGTGCGCAC GTTACCCTAT CGCATGTGCA AATAGCATCG GACTCTTGTG CACTATACCT
    TATACCAGGA GTGAAGAGAA GTGCCTGGTG CGGAGTGGCC TTGTCCAGCT CATGGATCGA
    CTATGCAGCT TGAGCAGTCA GACTGAGTCC AGCTCCAGTG AGAAACAAAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCCTG GGCAGCCTTC CAGGTGCTTG CCAACCGCTG CGTTGAATGG
    GAAAAAGAAG AAGGCGGCTC CACAGAGGCT GTGCACTCAG GTCTGGCCCG TCAGGTGTCC
    AGCCTTCTCA CGAATCACCT TGCCCGAGCG ACCGAGTGCT GTGGCAACCA GGCTGCCGGG
    AACGATGCCC TCCAGGACGT CCTGAGTCTC CTCAATGATC TCTCGAGGAG TCATATAGGT
    AAAGCCATCC TGAGCCAGCC AGCTTGTGTG TCCAAACTCC TCTCCCTGCT ACTTGACCAA
    CGCCCATCCC CAAAGCTGGT TCTTATCATT CTTCAGCTGT GCCGGGCGGC ACTCCCGCTG
    ATGAGCGTAG AAGACTGTGG GAACGTGGAG CTCCCACCCT GGAGCTACTC CGTCCCCTCC
    CTAAACAGCG AGCAGGAGGA TCCCAGCGAC CCAGCGTCCA AGATCGCCTC GCTGCTCTTG
    GCAAAGCTGG CAGACTACGT GGTCCCAGGA TGTCAGACAG TCCTTTCTCC AACCGCTTCC
    GAACCTGACA CCACATTGAC AAAAACCAGC CCCAAGAATT CCTTAAAAGG CGATAAAGAT
    CCTGGAGAAG AGAGTGAAGC AGTGGATGGC AAGCTCTCTA TCTTCATCCA CAAGCGGGAA
    GACCAGTCGT CCCATGAAGT TCTGCAGCCA TTACTAAGTA GCTCAGAAGG ACGGCCCTTC
    CGACTTGGTA CTGGTGCCAA CATGGAGAAA GTTGTGAAAA TGGACCGAGA CATGACGAAG
    GGTGGCTGCT GTGAGGTGAT TACGGAAGAG GCGGCAGCTG CTCTGCGAAA AGCGACCAAG
    TGGGCACAGT CAGGCCTCAT TGTCAGTGTT GGGCCACCCG TGGAGTCCCT CAACCCAGAG
    GCCGTGAGCG GGCTGTCCAC AGGGGACAAG AAGAAGACTG CCCAAACCTC CATTTGCCGA
    GAGAGAAACT CTGAACTCGC CAGGACCGAC CCCGTCCGAC CTTTCATCAG TGGGCACGTG
    GCAAACAGCA TGGCTGCTGA GGTGATCGCC TTGCTTCATA GCTTGCTGAT GGCACCTGAG
    TCAAATGCTG CTCAGATATG GACCACGACA GCAGAGAAGG TTCTGTCTCG TGCACTGATG
    TACATTCCAC AGTTGGGGAA ATATGCAGAA AGCATTCTGG AAAATGGCAG CAGCAGTGGC
    AGAAAGCTTG CCAAACTTCA GAGAATTGCC CGCCAGGCTG TGGCTGCACT CTGTGCACTT
    GGAGGCTTCA AAGAGACAAT CAAGATCGGG TCTGAGGTCC AGGTTTTAGG TAGAGGAATA
    TCAGGAAGCA TCGGAGTGGT GGCTTCTATC AACGAACAGG AAGGTATAGC TACGGTCAGA
    TTCCCACCCA TAGACTGTAG AAAGACTTCG CAAGCATCAG ACACACTGAC TATTCCATTG
    TCTAGACTTT GTGTTCCAAG ATCAGAGGCA TTGCCTCTTC ATAAACTGTC CATTACTGAG
    AAGGTAGTAC AGGCAGTCCA GTCCATGTTG CTTCCTCAGG AGGGAAGTCT CTCTATTCAT
    ACCTCACTTC CTGCAGCAGG AGATGGGTCA GCTCCTGTGA TGGCAGTCGT TCGGCTCCTG
    GCTGAAATAA GAACAAGAGC ATGCCTGGTC ATGGCCCAGC TTTTAGAAGA CAGCTTATTT
    TGTGAAGAAT TCATACAGCA GTGTCCAGCA GCTGTTGAAG TTCTTAACCT AGTAGCCCAG
    GAATGCAGTG CTGGAGAGCG GCTGGCAGTT GTGGAAGTGC AGTGTGAACG GCTGAGGATG
    CTCTACCGGG ACTGTGCTCG GCCCCCGCCG CCTCCGCTGC AGGCTGACCG GAGACAGCCC
    AAAGAGATTA CGTGGAGCCC CTCACGAGTG TTTCCACCTG TCCGAGCCTG CATGTTCTCC
    TCGCACCTCA CTTCTGTCAC CTTCCTAGCA GACCCCAGTG CAGGGGGCGG TCTGCCCAGG
    GGCACATTTA TCTACGCCAC CTCACCACTG CCCGTTCAGG CCCCGTCTTT CTACTGGGAA
    ATTGAAATTG TTTCCTATGG AGATACAGAT GATGATACTG GACCAATAGT TTCCTTTGGC
    TTTGCTACAG AAGCAGAGAA AAGAGATGGG GCATGGACTA ATCCAGTGGG CACCTGTCTT
    TTCCATAACA ATGGCCGAGC TGTGCATTAC AATGGCTCCA GTTTATTACA GTGGAAGAGC
    GTTCGATTAG ACGTAACTCT CTCTCCTGGT GACGTGGCAG GGATTGGCTG GGAGAGAACC
    GAGGGGACGC CTCCTCCACC CGGGCAGCCT GCCAAGGGCC GTGTGTACTT CACATACTGT
    GGGCAGCGCC TCTCACCGTA TCTCGAAGAT GTCTCGGGTG GGATGTGGCC GGTGGTTCAT
    ATTCAGAAAA AGAACACCAA GACTCGCGCT AATTTTGGCT CCCGCCCATT TGCCTACGCT
    GAAGGGCAAG CCCACCGCAA TGCTGCCGAC CTGTGCACTG ACCTGGCCGA GGAAATTAGC
    GCTAACTTCG AGGCCCTGCC CTTTGCCATG GCCTCTGACA GTGATAACGA TGCTGGCACC
    AGTATTGCGT CAGACCCGGG CACTCATGGG CCACCATGCC GAATTGCTGC CGTAGCCACT
    GCTCAGCAAC AATATGATAG TGATACTTCC TGTCATTATA AAGTGGAGCT CAGCTATGAG
    AATTTCATCA CCTCTGGGCC AGACCCTCAC CCACCCCCCA TTGCAGATGA CGAAAGTGAT
    GATGATGATG ACGATGACAT CCCACAGGAG GATCACTATG CCCTGCTGGT GAAAGCCTGG
    GAGACCAAGG TTTTTCCTAC CATCCGAAGA CGGTTCCGCA ACGAAGCAGA GAGGAAATCA
    GGACTGGACC AGATAAAGGG TGCTTTACAA CTAGGCATGG TGGATATTGC CCGGCAGACA
    GTTGAATTCC TCTACGAGGA GAATGGTGGC ATCCCAAGAG ACCTTTATCT TCCCACCATT
    GAAGACATTA AAGATGAAGC GAACAAGTTC ACAATTGATA AAGTTCGAAA AGGTCTCACG
    GTAGTAACAC GCTCTCCAGA CAGCAATAAC GTAGCCAGCA GCACTGTTGG AACTGCCCTG
    CCAAAATTTG CTATCCGAGG GATGCTGAAA ACCTTTGGGC TTCATGGAGT TGTCTTAGAT
    GTTGATTCAG TTAATGAACT AGTTCAGGTA GAAACGTACC TCCGAAGTGA AGGTGTGCTG
    GTCCGATACT GGTACCCTAT TGATATGTTG GAAAGGCCCC CCGCAGGCTA CAGAAGGACT
    GCCACCAATG GGCTCGTCAC ACTGGACAAT ACCAACCTTC AAATTCACAG GGAGCTGCTG
    CGCTGCGAGG CCGCGCTGGC CAGGCTGTAC TGCCGCATGG CCCTGCTCAA CATCTTCGCC
    CCCAAGCTGC CTCACCTGTT TACCCGCCTC TTCCACATCC CCGCCATCCG GGACATTACC
    CTGGAGCACC TGCAGCTGCT GTCCAATCAG CTCCTCGCTC CTCCCCTCCC AGATGGCACC
    ATCAGCTCCA GCTCCATCCT CCTGGCGCAG TCCCTGCAGC ACTGTGTCCA TTCCCAGAAC
    TGTTCGGCCA CAGACCTCTT CTACCAGGGC AGCTCCCAGA CGGTGCGAGA GTGGCTCAAC
    GTGGCCATCA CGCGGACCCT GCACCAGGGC GAGGAAAGCC TTCTGGAGCT GACCAAACAG
    ATCTGCTCTT TCCTGCAGAC AGCACCAGAA CAGTTCCCCT CCGAAGAGTT TCCAATTTCC
    GAGTCCAAAG TCAACATGGA CGTCAATTTC CCCGGGGCAG CTTTCGTCGT CGTATCTTGC
    AAAGAAAGTC AGTCTGGATT CCGCAAAGAT TCCTCTCTGT ACAAGGCACC ATGGGCGCGT
    GTGCTGGTGT ACGGGCTAGG CCACAAAGTG AAGCGAAATG GCCAGCTGAA CCTCATTGAG
    GCCGTCTGTT ACCCGCGGGA TGCGTCTCCG GCCAACACAG GGCTCACACC ACCTCCTACC
    ACCAACCAGT ACCCTTCTGT GATCCTCTCC ACAGACAAAG TCCACATCAA ACTGGGGGTA
    TCTCCCCCAC CCGGAGCCGT GCTGGTGTTA CACTCCCTGC CTCTCGAGTT CCCCCTGGCG
    ATGGCCTTCA CCGAGCAGCT ACTGTCCTGG AAATCAGAGG ATGGCGAAGG TAGAGCTGAA
    GATGAGCCTG ACACCATCCC GACGTCCGTC CTCCTGCAAG TGGTGGAGCT GCTTGGCAAC
    TTCCTCTGGA CCACAGACAT GGCGGCCTGC GTGAAGGAGC TGGTTTTCCA CCTCCTGGCA
    GAGCTCCTGC GCACGGTGCA CAGCCTAGAG CAGAGGAAGC ACCCAGCCGG CCTGTCCTCA
    TCCATCGCCC TCCAGCTGAA CCCCTGCCTG GCCATGCTGA TGGCCCTGCA GTCGGAGCTT
    CACAAGCTGT ACGACGAGGA GACGCAGAGC TGGGTCTCGG GCAGTGCATG CGGAGGCTCA
    GGAGCCACGG CGGCTGCTGA CCAGGGCCGG TTCTCGACGT ATTTCCACGC ACTCATGGAG
    GGCTGCCTGG CGGTGGCCGA GGTGACCCTG CCCACCAACA TGAGTGTCAC CGCCAGCGGG
    GTGACGTCAG CAACTGCCCC AAACCTCAGC GACTCGTCCT CCTCCTCATC GTCCTCCCCA
    GGACAGACTC CACAGAGCCC CAGCCTCCTG TCCAAGAGGA AGAAAGTCAA GATGAAGCGG
    GAAAAGGCCT CCTCCTCTGG GAAGCGCCAG TCCTCACGCT CTGTGGACTC GGACCCCGCC
    GTGCTGAGCA TCGGGGGCAG CAAGCCCGAG GACATGCTGT GGTTCCATCG TGCTCTGACG
    TTGCTCATCA TCCTCCGCCA CCTCACCAGG AAGGACCCAC AGGGGCTAGG CGTGACAAGC
    GACGCCATCG CCGACGCCTG CCAGGCCCTG GTGGGCCCCA CCGCCCACAG CCGCTTGCTG
    GTGATCTCTG GGATCCCCAC CCACCTGGAC GAGGGCATCG TCAGGGGTGC CATCCGCAAG
    GCCTGCAATG CCCATGGCGG GGTGTTCAAG GATGAGATCT ACATCCCCCT GCAGGACGAA
    GACCCCAAGA AGCCAAAAGA TAAGGCCGAG GGGGGTGATG GGAAAGCCGA GCCGGAAAAG
    ACCCTCGGCT TCCCCAGCAC GGACAGCTTG GAGGTCAGCA CATCCAGCAG CCTGACCCCC
    GCTATGAGCG TCAGCGCATC CGCGTCCACC AGCCAGGCTT CCATCTGCAG CTCCCAGGGC
    ATCTCGCAGA CAGTCAGTGA CCTCTCTGCG GATCCGTTGC CTGCAGGCCT GGAGCTGCCC
    ATCCCTGCAG GCTTGTTGGA GCCCCATGTG GTGTCCAGCC AGGAGAGCCT GGACATTTCC
    CTGTGTAGCA CCGGCAGCCT GGGCAGCCTG GGGAGCCTGG GCGAGCCACT GGACAATGCA
    GAAACGGCCT CAGTGTCAGA CATGGGCTCC ATGTACACAG TCACGTCCCT GGACAACCAG
    CCCCTTGCAG CGCGCCCCAT CAAAGGCTTC GCGGTCGTGG AGATAAGGTC CCGAGCCAAA
    ATTGAGAAAA TTCGAGCAAG TTTATTTAAC AACAATGACT TGATAGGTTT GTCAAGCTTG
    GATGGTGAGG ATGAATTGAT GGAAATGTCA ACGGAAGAGA TCCTCACGGT GTCTGTAGTG
    AATCAGAGTC TATTCGATAC GCAGGGGAGC CCAGGGTTAG AAGATTATTT CAATGATAAG
    TCAATTAAAG GTGAGAAGCT GGTGCCTGGG GCCAGAGAGG TGCTGACGGA GATATTTAAG
    AGCTGTGCCC ACTCGGAGCA GATGCTGAGC CTGACGCCAG CAAAGCCCAT CAAAGTGTCG
    GACATTTATC TTAGCAAAGA GCAGATCAAC TCCCAGACAC CGGGCAACCT CCTCCACCTT
    TTCTTCACCA ATGTCCGGCC CCCCAAAAAG GTGCTAGAGG ACCAGCTCAC CCAGATCCTG
    AGGAAGTACG GTGTGCCGAA GCCCAAGTTT GACAAGAGCA AGTACAGCAA GGCGGGGAAG
    GAGCAGCACC CCGGGAAGGT GGTGAGCACC AAGCGGCCCA TCACCAAGCC GCCCACCAAG
    GACAAGGCAG TGCTCAACAG CGTCAGCAGG ACTGCCTTGA GTGAGAAGAA GCCCACCGTG
    AAGCCCAAGT CACCTGAAAA GAGCAAGCCA GATGAAAAGG ACCCTGAAAA GTCACCCACC
    AAGAAACAAG ACGTCCCAGA GGAGAAGTAC CTGACCTTAG AAGGATTTCA CAAGTTTGTG
    ATCGACCGAG CCAAGCAAGA CATCCGCAGC GTCTGGAGAG CGATTTTATC CTGTGGCTAC
    GATCTTCATT TTGAGAGGTG TGCCTGCATC GACGTCCGGC ATGCGCACAA GGCCTCCAGG
    AAGTGGACCC TGGAGATGGA TGTGGCGCTC GTGCAGTACA TCAACCGGCT GTGCCGCCAC
    CTGGCCATCA CGCCCGCGCG TCTGCATCCC CACGAGGTGT ATCTGGACCC TGCAGACGCT
    GCTGACCCCA GAGTGGCCTG TCTCTTGAAC GTGCCCATCG AGAGCCTGCG CCTGCGCTTC
    GCTCTGCTGC AGTCCCTCAA CACCACGCTC GAGACCTTCT TCCTGCCGCT GGTTGAGCTG
    CGCCAGACTC CCATGTACAC GCACAGCATC GCCGCCCTGC TCAAGGAGGC CAAAGGGCTG
    ATCTTTTATG ACACAAAGGT GACCGTCATG AATCGGGTGC TAAATGCCAC GGTGCAGAGG
    ACGGCTGACC ATGCAGCACC AGAAATCACT CTGGACCCGC TGGAGATCGT GGGAGGGGAG
    ATCAGAGCCT CTGAGAACTC CTACTTCTGC CAGGCAGCCA GGCAGCTGGC CTCAGTGCCC
    TCCTCCCAGC TTTGCGTCAA ACTGGCGAGT GGCGGCGACC CTACCTATGC CTTCAACATC
    CGCTTCACGG GAGAGGAGGT CCATGGCACC AGTGGCTCCT TCCGCCACTT CCTGTGGCAG
    GTGTGCAGAG AGCTGCAGAG CTCCTCGCTC TCCCTCCTCC TGCTGTGCCC CAGCTCCGCC
    GTCAACAAGA ACAAGGGCAA GTACATCCTG ACCCCCAGCC CCATCACCTA TGGGGAGGAG
    CAGCTGCTGC ACTTCCTGGG CCAGTTGCTA GGGATCGCCA TCCGAGCAGA TGTCCCCCTC
    CCCCTGGACC TCCTGCCCTC CTTCTGGAAG ACACTGGTGG GCGAGCCCCT GGACCCTGAC
    CAAGACCTGC AGGAAGCAGA CGTACTCACC TACAATTACG TCAAGAAATT TGAGAGTATC
    AACGACGAGA CGGAGTTGGA GGCCCTGTGT GCTGAGATCG CCTCCCAGCA CCTGGCCACT
    GAGAGCCCCG AGGGGCCCAA CAAGCCCTGC TGCCGGTTCA CCTACCTGAC CATGACCGGC
    GAGGAGGTGG AGCTGTGCAG CCGTGGCCGG CACATCCCCG TGGCGTGGGA GAACAAGGAC
    ATCTATGCGG CCGCCATCCG GAGCCTGCGG CTGCGTGAGC TACAGAACGT GGAGTGTGTG
    ACGGCCGTGC GCGCGGGCCT TGGGGCCATC ATCCCCCTGC AGCTGCTCAC CATGCTCAGC
    CCACTGGAGA TGGAGCTGCG CACCTGCGGC CTCCCGTACA TCAACCTCGA GTTCCTGAAG
    GCCCACACCA TGTACCAGGT GGGGCTGATG GAGACCGACC AGCACATCGA GTTCTTCTGG
    GGGGCACTGG AGATGTTTGC CCAGGAGGAG CTGTGCAAGT TCATCAAGTT TGCCTGCAAC
    CAGGAGCGCA TCCCGTTCAC CTGCCCCTGC AAAGACGGGG GTCCTGACAC GGCCCATGTG
    CCCCCATACC CCATGAAGAT TGCCCCCCCA GATGGCACAG CAGGTTCCCC AGATTCTCGC
    TACATCCGAG TGGAGACCTG TATGTTCATG ATCAAGCTTC CCCAGTACTC CTCGCTGGAA
    ATCATGCTGG AGAAACTACG TTGTGCCATT CACTACCGCG AAGACCCTCT GAGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322650.1
    CDS1025..12724
    Translation

    Target ORF information:

    RefSeq Version XM_015467164.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TTGGTGATAT ACGCGGGGCC TAACACAAAC
    AGTAGGAAGG TTGCTGAGTA TGGAGGCAAT ACACTGGGAT ATGGCAGCCG TAGTGTCTTA
    GGAACTGGTT GGCCGAAAGA CTTGGTGAAG GTGGAAGGAG ATACAGTCAC CTTCTCCTTT
    GAAATGAGAA GTGGCCGTGA ACACAACACT CCCGATAAGG CTATGTGGGG CTTTGCTTGC
    ACAGTTCGCG CTCAGGAGTC TTCGGAGGAT GTGTCAGGAG GCTTACCCTT TCTGGTAGAC
    CTGGCTTTAG GTCTATCTGT GTTAGCTTGT TCCATGTTAA GAATCCTATA CAACGGACCA
    GAAATTACCA AAGAAGAAGA AACCTGTCAG GAGCTGTTGC GGTCCAAACT TTTACAAAGG
    TGCCAGTGGC AGGTAGAGGC CAACGGTGTG ATCTCCCCTG CCCTTACTCC AAGCCCATCT
    CCACTGCCTC TGACCATCGA GGAAGACAGA GAGTTCACTT ACCCGTCTGA TGTCCTCGTG
    CCTCCTGTTG GAAACTACTT TGACCTGCCC CGGATCAGGC TGCCTCCAGG AATCATGATA
    AAGCTCAGGG AAATTTCTGG GCGTGCTAGA CCACAGTTTA GACCAAGTAT AAAAGAAGTA
    ATTCAGCCAG ATGTGATGGA GGAAATGGTG GTATCATGTG TTATTAAGCA CTTGAACTTA
    GTTGATGCAC TGCAGTCCCT AATAAATTTC CAGTATCAAG AAGAACATGC TGAGGAATAT
    GATTTACTGT GTAAAATCAT GGGAGAGACA TTTAAGAAAC TCAATGCCAT GGAGAGACAG
    CTGCAGAGTG TTGCAGAATT GGAACAGAAA TGGCAGAGTG AAGTTGATGA TGCCATGCAG
    GGGAAACTGG AGAACAACAT GCCTTTCTTC TATGATTACC ATTTCAATGA GAACAAAATG
    AAAGAACTAG AACTTTTGTG TTCAATGAAG GAAGTCTCCT TTGATGGAAA TGATCTTGAA
    AACATGGTGC TATCTCTGAG GGAGAAGTTC CTACAAGAAG TGAATTCTCT TATTCAGAAA
    CCCTCGCACC CACTGGCTAA AACGAAGACG TTAGTGAAGA GTCTAATGAA CCGAGCTGAG
    CTGTTGCTGC ACGTCACCAT TGCAGCCCAG TCTGGCCTCA CGAGAAGCAT CTCAGGGACC
    CCCGCGGAAA CACCGGCTTG TAAATCAGCC TCTGAAACAA AGGTGATCTC TCATGCTGTG
    CGTCAGCCTG TTTTCCTTCG CAGCATGTCG GCTCCCTCTG ACCTGGAAAT GATCGGTAAT
    GAAGATTTGG AATTTACTAG AGCAAATCAG AGGCGGCGCC ACGTGACCAG CCACCGCAGC
    AGCTCCTTCA CACTCCTGCA GTCATTGGCC ATAGAGGACA GCAGGGACAA GCCCACCTAC
    AGCGTCCTGC TGGGGCAGCT GTTTGCTTTC ATCGGCACCA ACCCTGACCA AGCTGTGTCC
    AGCAGCAGCT TTCTTCTGGC CGCACAGACG AGATGGCGGC GGGGAAACAC CCGCAAGCAG
    GCGTTGGTGC ACATGCGGGA GCTGCTGACG GCTGCCGTGC GAGTCGGGGG AGTGACACAC
    CTTGTGGGTC CAGTGACAAT GGTCCTGCAG GGAGGACCCA GAATTGAAGA ACTGACCTGT
    GGTGGGATGG TCGAGCAGGT CCAGGAAGCC TTCGGTGAGA CCATGACCTC TGTGGTGTCT
    TTGTGCGCAC GTTACCCTAT CGCATGTGCA AATAGCATCG GACTCTTGTG CACTATACCT
    TATACCAGGA GTGAAGAGAA GTGCCTGGTG CGGAGTGGCC TTGTCCAGCT CATGGATCGA
    CTATGCAGCT TGAGCAGTCA GACTGAGTCC AGCTCCAGTG AGAAACAAAC CAAGAAGCAG
    AAGGTGGCCA CCATGGCCTG GGCAGCCTTC CAGGTGCTTG CCAACCGCTG CGTTGAATGG
    GAAAAAGAAG AAGGCGGCTC CACAGAGGCT GTGCACTCAG GTCTGGCCCG TCAGGTGTCC
    AGCCTTCTCA CGAATCACCT TGCCCGAGCG ACCGAGTGCT GTGGCAACCA GGCTGCCGGG
    AACGATGCCC TCCAGGACGT CCTGAGTCTC CTCAATGATC TCTCGAGGAG TCATATAGGT
    AAAGCCATCC TGAGCCAGCC AGCTTGTGTG TCCAAACTCC TCTCCCTGCT ACTTGACCAA
    CGCCCATCCC CAAAGCTGGT TCTTATCATT CTTCAGCTGT GCCGGGCGGC ACTCCCGCTG
    ATGAGCGTAG AAGACTGTGG GAACGTGGAG CTCCCACCCT GGAGCTACTC CGTCCCCTCC
    CTAAACAGCG AGCAGGAGGA TCCCAGCGAC CCAGCGTCCA AGATCGCCTC GCTGCTCTTG
    GCAAAGCTGG CAGACTACGT GGTCCCAGGA TGTCAGACAG TCCTTTCTCC AACCGCTTCC
    GAACCTGACA CCACATTGAC AAAAACCAGC CCCAAGAATT CCTTAAAAGG CGATAAAGAT
    CCTGGAGAAG AGAGTGAAGC AGTGGATGGC AAGCTCTCTA TCTTCATCCA CAAGCGGGAA
    GACCAGTCGT CCCATGAAGT TCTGCAGCCA TTACTAAGTA GCTCAGAAGG ACGGCCCTTC
    CGACTTGGTA CTGGTGCCAA CATGGAGAAA GTTGTGAAAA TGGACCGAGA CATGACGAAG
    GGTGGCTGCT GTGAGGTGAT TACGGAAGAG GCGGCAGCTG CTCTGCGAAA AGCGACCAAG
    TGGGCACAGT CAGGCCTCAT TGTCAGTGTT GGGCCACCCG TGGAGTCCCT CAACCCAGAG
    GCCGTGAGCG GGCTGTCCAC AGGGGACAAG AAGAAGACTG CCCAAACCTC CATTTGCCGA
    GAGAGAAACT CTGAACTCGC CAGGACCGAC CCCGTCCGAC CTTTCATCAG TGGGCACGTG
    GCAAACAGCA TGGCTGCTGA GGTGATCGCC TTGCTTCATA GCTTGCTGAT GGCACCTGAG
    TCAAATGCTG CTCAGATATG GACCACGACA GCAGAGAAGG TTCTGTCTCG TGCACTGATG
    TACATTCCAC AGTTGGGGAA ATATGCAGAA AGCATTCTGG AAAATGGCAG CAGCAGTGGC
    AGAAAGCTTG CCAAACTTCA GAGAATTGCC CGCCAGGCTG TGGCTGCACT CTGTGCACTT
    GGAGGCTTCA AAGAGACAAT CAAGATCGGG TCTGAGGTCC AGGTTTTAGG TAGAGGAATA
    TCAGGAAGCA TCGGAGTGGT GGCTTCTATC AACGAACAGG AAGGTATAGC TACGGTCAGA
    TTCCCACCCA TAGACTGTAG AAAGACTTCG CAAGCATCAG ACACACTGAC TATTCCATTG
    TCTAGACTTT GTGTTCCAAG ATCAGAGGCA TTGCCTCTTC ATAAACTGTC CATTACTGAG
    AAGGTAGTAC AGGCAGTCCA GTCCATGTTG CTTCCTCAGG AGGGAAGTCT CTCTATTCAT
    ACCTCACTTC CTGCAGCAGG AGATGGGTCA GCTCCTGTGA TGGCAGTCGT TCGGCTCCTG
    GCTGAAATAA GAACAAGAGC ATGCCTGGTC ATGGCCCAGC TTTTAGAAGA CAGCTTATTT
    TGTGAAGAAT TCATACAGCA GTGTCCAGCA GCTGTTGAAG TTCTTAACCT AGTAGCCCAG
    GAATGCAGTG CTGGAGAGCG GCTGGCAGTT GTGGAAGTGC AGTGTGAACG GCTGAGGATG
    CTCTACCGGG ACTGTGCTCG GCCCCCGCCG CCTCCGCTGC AGGCTGACCG GAGACAGCCC
    AAAGAGATTA CGTGGAGCCC CTCACGAGTG TTTCCACCTG TCCGAGCCTG CATGTTCTCC
    TCGCACCTCA CTTCTGTCAC CTTCCTAGCA GACCCCAGTG CAGGGGGCGG TCTGCCCAGG
    GGCACATTTA TCTACGCCAC CTCACCACTG CCCGTTCAGG CCCCGTCTTT CTACTGGGAA
    ATTGAAATTG TTTCCTATGG AGATACAGAT GATGATACTG GACCAATAGT TTCCTTTGGC
    TTTGCTACAG AAGCAGAGAA AAGAGATGGG GCATGGACTA ATCCAGTGGG CACCTGTCTT
    TTCCATAACA ATGGCCGAGC TGTGCATTAC AATGGCTCCA GTTTATTACA GTGGAAGAGC
    GTTCGATTAG ACGTAACTCT CTCTCCTGGT GACGTGGCAG GGATTGGCTG GGAGAGAACC
    GAGGGGACGC CTCCTCCACC CGGGCAGCCT GCCAAGGGCC GTGTGTACTT CACATACTGT
    GGGCAGCGCC TCTCACCGTA TCTCGAAGAT GTCTCGGGTG GGATGTGGCC GGTGGTTCAT
    ATTCAGAAAA AGAACACCAA GACTCGCGCT AATTTTGGCT CCCGCCCATT TGCCTACGCT
    GAAGGGCAAG CCCACCGCAA TGCTGCCGAC CTGTGCACTG ACCTGGCCGA GGAAATTAGC
    GCTAACTTCG AGGCCCTGCC CTTTGCCATG GCCTCTGACA GTGATAACGA TGCTGGCACC
    AGTATTGCGT CAGACCCGGG CACTCATGGG CCACCATGCC GAATTGCTGC CGTAGCCACT
    GCTCAGCAAC AATATGATAG TGATACTTCC TGTCATTATA AAGTGGAGCT CAGCTATGAG
    AATTTCATCA CCTCTGGGCC AGACCCTCAC CCACCCCCCA TTGCAGATGA CGAAAGTGAT
    GATGATGATG ACGATGACAT CCCACAGGAG GATCACTATG CCCTGCTGGT GAAAGCCTGG
    GAGACCAAGG TTTTTCCTAC CATCCGAAGA CGGTTCCGCA ACGAAGCAGA GAGGAAATCA
    GGACTGGACC AGATAAAGGG TGCTTTACAA CTAGGCATGG TGGATATTGC CCGGCAGACA
    GTTGAATTCC TCTACGAGGA GAATGGTGGC ATCCCAAGAG ACCTTTATCT TCCCACCATT
    GAAGACATTA AAGATGAAGC GAACAAGTTC ACAATTGATA AAGTTCGAAA AGGTCTCACG
    GTAGTAACAC GCTCTCCAGA CAGCAATAAC GTAGCCAGCA GCACTGTTGG AACTGCCCTG
    CCAAAATTTG CTATCCGAGG GATGCTGAAA ACCTTTGGGC TTCATGGAGT TGTCTTAGAT
    GTTGATTCAG TTAATGAACT AGTTCAGGTA GAAACGTACC TCCGAAGTGA AGGTGTGCTG
    GTCCGATACT GGTACCCTAT TGATATGTTG GAAAGGCCCC CCGCAGGCTA CAGAAGGACT
    GCCACCAATG GGCTCGTCAC ACTGGACAAT ACCAACCTTC AAATTCACAG GGAGCTGCTG
    CGCTGCGAGG CCGCGCTGGC CAGGCTGTAC TGCCGCATGG CCCTGCTCAA CATCTTCGCC
    CCCAAGCTGC CTCACCTGTT TACCCGCCTC TTCCACATCC CCGCCATCCG GGACATTACC
    CTGGAGCACC TGCAGCTGCT GTCCAATCAG CTCCTCGCTC CTCCCCTCCC AGATGGCACC
    ATCAGCTCCA GCTCCATCCT CCTGGCGCAG TCCCTGCAGC ACTGTGTCCA TTCCCAGAAC
    TGTTCGGCCA CAGACCTCTT CTACCAGGGC AGCTCCCAGA CGGTGCGAGA GTGGCTCAAC
    GTGGCCATCA CGCGGACCCT GCACCAGGGC GAGGAAAGCC TTCTGGAGCT GACCAAACAG
    ATCTGCTCTT TCCTGCAGAC AGCACCAGAA CAGTTCCCCT CCGAAGAGTT TCCAATTTCC
    GAGTCCAAAG TCAACATGGA CGTCAATTTC CCCGGGGCAG CTTTCGTCGT CGTATCTTGC
    AAAGAAAGTC AGTCTGGATT CCGCAAAGAT TCCTCTCTGT ACAAGGCACC ATGGGCGCGT
    GTGCTGGTGT ACGGGCTAGG CCACAAAGTG AAGCGAAATG GCCAGCTGAA CCTCATTGAG
    GCCGTCTGTT ACCCGCGGGA TGCGTCTCCG GCCAACACAG GGCTCACACC ACCTCCTACC
    ACCAACCAGT ACCCTTCTGT GATCCTCTCC ACAGACAAAG TCCACATCAA ACTGGGGGTA
    TCTCCCCCAC CCGGAGCCGT GCTGGTGTTA CACTCCCTGC CTCTCGAGTT CCCCCTGGCG
    ATGGCCTTCA CCGAGCAGCT ACTGTCCTGG AAATCAGAGG ATGGCGAAGG TAGAGCTGAA
    GATGAGCCTG ACACCATCCC GACGTCCGTC CTCCTGCAAG TGGTGGAGCT GCTTGGCAAC
    TTCCTCTGGA CCACAGACAT GGCGGCCTGC GTGAAGGAGC TGGTTTTCCA CCTCCTGGCA
    GAGCTCCTGC GCACGGTGCA CAGCCTAGAG CAGAGGAAGC ACCCAGCCGG CCTGTCCTCA
    TCCATCGCCC TCCAGCTGAA CCCCTGCCTG GCCATGCTGA TGGCCCTGCA GTCGGAGCTT
    CACAAGCTGT ACGACGAGGA GACGCAGAGC TGGGTCTCGG GCAGTGCATG CGGAGGCTCA
    GGAGCCACGG CGGCTGCTGA CCAGGGCCGG TTCTCGACGT ATTTCCACGC ACTCATGGAG
    GGCTGCCTGG CGGTGGCCGA GGTGACCCTG CCCACCAACA TGAGTGTCAC CGCCAGCGGG
    GTGACGTCAG CAACTGCCCC AAACCTCAGC GACTCGTCCT CCTCCTCATC GTCCTCCCCA
    GGACAGACTC CACAGAGCCC CAGCCTCCTG TCCAAGAGGA AGAAAGTCAA GATGAAGCGG
    GAAAAGGCCT CCTCCTCTGG GAAGCGCCAG TCCTCACGCT CTGTGGACTC GGACCCCGCC
    GTGCTGAGCA TCGGGGGCAG CAAGCCCGAG GACATGCTGT GGTTCCATCG TGCTCTGACG
    TTGCTCATCA TCCTCCGCCA CCTCACCAGG AAGGACCCAC AGGGGCTAGG CGTGACAAGC
    GACGCCATCG CCGACGCCTG CCAGGCCCTG GTGGGCCCCA CCGCCCACAG CCGCTTGCTG
    GTGATCTCTG GGATCCCCAC CCACCTGGAC GAGGGCATCG TCAGGGGTGC CATCCGCAAG
    GCCTGCAATG CCCATGGCGG GGTGTTCAAG GATGAGATCT ACATCCCCCT GCAGGACGAA
    GACCCCAAGA AGCCAAAAGA TAAGGCCGAG GGGGGTGATG GGAAAGCCGA GCCGGAAAAG
    ACCCTCGGCT TCCCCAGCAC GGACAGCTTG GAGGTCAGCA CATCCAGCAG CCTGACCCCC
    GCTATGAGCG TCAGCGCATC CGCGTCCACC AGCCAGGCTT CCATCTGCAG CTCCCAGGGC
    ATCTCGCAGA CAGTCAGTGA CCTCTCTGCG GATCCGTTGC CTGCAGGCCT GGAGCTGCCC
    ATCCCTGCAG GCTTGTTGGA GCCCCATGTG GTGTCCAGCC AGGAGAGCCT GGACATTTCC
    CTGTGTAGCA CCGGCAGCCT GGGCAGCCTG GGGAGCCTGG GCGAGCCACT GGACAATGCA
    GAAACGGCCT CAGTGTCAGA CATGGGCTCC ATGTACACAG TCACGTCCCT GGACAACCAG
    CCCCTTGCAG CGCGCCCCAT CAAAGGCTTC GCGGTCGTGG AGATAAGGTC CCGAGCCAAA
    ATTGAGAAAA TTCGAGCAAG TTTATTTAAC AACAATGACT TGATAGGTTT GTCAAGCTTG
    GATGGTGAGG ATGAATTGAT GGAAATGTCA ACGGAAGAGA TCCTCACGGT GTCTGTAGTG
    AATCAGAGTC TATTCGATAC GCAGGGGAGC CCAGGGTTAG AAGATTATTT CAATGATAAG
    TCAATTAAAG GTGAGAAGCT GGTGCCTGGG GCCAGAGAGG TGCTGACGGA GATATTTAAG
    AGCTGTGCCC ACTCGGAGCA GATGCTGAGC CTGACGCCAG CAAAGCCCAT CAAAGTGTCG
    GACATTTATC TTAGCAAAGA GCAGATCAAC TCCCAGACAC CGGGCAACCT CCTCCACCTT
    TTCTTCACCA ATGTCCGGCC CCCCAAAAAG GTGCTAGAGG ACCAGCTCAC CCAGATCCTG
    AGGAAGTACG GTGTGCCGAA GCCCAAGTTT GACAAGAGCA AGTACAGCAA GGCGGGGAAG
    GAGCAGCACC CCGGGAAGGT GGTGAGCACC AAGCGGCCCA TCACCAAGCC GCCCACCAAG
    GACAAGGCAG TGCTCAACAG CGTCAGCAGG ACTGCCTTGA GTGAGAAGAA GCCCACCGTG
    AAGCCCAAGT CACCTGAAAA GAGCAAGCCA GATGAAAAGG ACCCTGAAAA GTCACCCACC
    AAGAAACAAG ACGTCCCAGA GGAGAAGTAC CTGACCTTAG AAGGATTTCA CAAGTTTGTG
    ATCGACCGAG CCAAGCAAGA CATCCGCAGC GTCTGGAGAG CGATTTTATC CTGTGGCTAC
    GATCTTCATT TTGAGAGGTG TGCCTGCATC GACGTCCGGC ATGCGCACAA GGCCTCCAGG
    AAGTGGACCC TGGAGATGGA TGTGGCGCTC GTGCAGTACA TCAACCGGCT GTGCCGCCAC
    CTGGCCATCA CGCCCGCGCG TCTGCATCCC CACGAGGTGT ATCTGGACCC TGCAGACGCT
    GCTGACCCCA GAGTGGCCTG TCTCTTGAAC GTGCCCATCG AGAGCCTGCG CCTGCGCTTC
    GCTCTGCTGC AGTCCCTCAA CACCACGCTC GAGACCTTCT TCCTGCCGCT GGTTGAGCTG
    CGCCAGACTC CCATGTACAC GCACAGCATC GCCGCCCTGC TCAAGGAGGC CAAAGGGCTG
    ATCTTTTATG ACACAAAGGT GACCGTCATG AATCGGGTGC TAAATGCCAC GGTGCAGAGG
    ACGGCTGACC ATGCAGCACC AGAAATCACT CTGGACCCGC TGGAGATCGT GGGAGGGGAG
    ATCAGAGCCT CTGAGAACTC CTACTTCTGC CAGGCAGCCA GGCAGCTGGC CTCAGTGCCC
    TCCTCCCAGC TTTGCGTCAA ACTGGCGAGT GGCGGCGACC CTACCTATGC CTTCAACATC
    CGCTTCACGG GAGAGGAGGT CCATGGCACC AGTGGCTCCT TCCGCCACTT CCTGTGGCAG
    GTGTGCAGAG AGCTGCAGAG CTCCTCGCTC TCCCTCCTCC TGCTGTGCCC CAGCTCCGCC
    GTCAACAAGA ACAAGGGCAA GTACATCCTG ACCCCCAGCC CCATCACCTA TGGGGAGGAG
    CAGCTGCTGC ACTTCCTGGG CCAGTTGCTA GGGATCGCCA TCCGAGCAGA TGTCCCCCTC
    CCCCTGGACC TCCTGCCCTC CTTCTGGAAG ACACTGGTGG GCGAGCCCCT GGACCCTGAC
    CAAGACCTGC AGGAAGCAGA CGTACTCACC TACAATTACG TCAAGAAATT TGAGAGTATC
    AACGACGAGA CGGAGTTGGA GGCCCTGTGT GCTGAGATCG CCTCCCAGCA CCTGGCCACT
    GAGAGCCCCG AGGGGCCCAA CAAGCCCTGC TGCCGGTTCA CCTACCTGAC CATGACCGGC
    GAGGAGGTGG AGCTGTGCAG CCGTGGCCGG CACATCCCCG TGGCGTGGGA GAACAAGGAC
    ATCTATGCGG CCGCCATCCG GAGCCTGCGG CTGCGTGAGC TACAGAACGT GGAGTGTGTG
    ACGGCCGTGC GCGCGGGCCT TGGGGCCATC ATCCCCCTGC AGCTGCTCAC CATGCTCAGC
    CCACTGGAGA TGGAGCTGCG CACCTGCGGC CTCCCGTACA TCAACCTCGA GTTCCTGAAG
    GCCCACACCA TGTACCAGGT GGGGCTGATG GAGACCGACC AGCACATCGA GTTCTTCTGG
    GGGGCACTGG AGATGTTTGC CCAGGAGGAG CTGTGCAAGT TCATCAAGTT TGCCTGCAAC
    CAGGAGCGCA TCCCGTTCAC CTGCCCCTGC AAAGACGGGG GTCCTGACAC GGCCCATGTG
    CCCCCATACC CCATGAAGAT TGCCCCCCCA GATGGCACAG CAGGTTCCCC AGATTCTCGC
    TACATCCGAG TGGAGACCTG TATGTTCATG ATCAAGCTTC CCCAGTACTC CTCGCTGGAA
    ATCATGCTGG AGAAACTACG TTGTGCCATT CACTACCGCG AAGACCCTCT GAGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52732
    Clone ID Related Accession (Same CDS sequence) XM_010814060.2 , XM_015467165.1
    Accession Version XM_010814060.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010814060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATTGGTG ATATACGCGG GGCCTAACAC AAACAGTAGG
    AAGGTTGCTG AGTATGGAGG CAATACACTG GGATATGGCA GCCGTAGTGT CTTAGGAACT
    GGTTGGCCGA AAGACTTGGT GAAGGTGGAA GGAGATACAG TCACCTTCTC CTTTGAAATG
    AGAAGTGGCC GTGAACACAA CACTCCCGAT AAGGCTATGT GGGGCTTTGC TTGCACAGTT
    CGCGCTCAGG AGTCTTCGGA GGATGTGTCA GGAGGCTTAC CCTTTCTGGT AGACCTGGCT
    TTAGGTCTAT CTGTGTTAGC TTGTTCCATG TTAAGAATCC TATACAACGG ACCAGAAATT
    ACCAAAGAAG AAGAAACCTG TCAGGAGCTG TTGCGGTCCA AACTTTTACA AAGGTGCCAG
    TGGCAGGTAG AGGCCAACGG TGTGATCTCC CCTGCCCTTA CTCCAAGCCC ATCTCCACTG
    CCTCTGACCA TCGAGGAAGA CAGAGAGTTC ACTTACCCGT CTGATGTCCT CGTGCCTCCT
    GTTGGAAACT ACTTTGACCT GCCCCGGATC AGGCTGCCTC CAGGAATCAT GATAAAGCTC
    AGGGAAATTT CTGGGCGTGC TAGACCACAG TTTAGACCAA GTATAAAAGA AGTAATTCAG
    CCAGATGTGA TGGAGGAAAT GGTGGTATCA TGTGTTATTA AGCACTTGAA CTTAGTTGAT
    GCACTGCAGT CCCTAATAAA TTTCCAGTAT CAAGAAGAAC ATGCTGAGGA ATATGATTTA
    CTGTGTAAAA TCATGGGAGA GACATTTAAG AAACTCAATG CCATGGAGAG ACAGCTGCAG
    AGTGTTGCAG AATTGGAACA GAAATGGCAG AGTGAAGTTG ATGATGCCAT GCAGGGGAAA
    CTGGAGAACA ACATGCCTTT CTTCTATGAT TACCATTTCA ATGAGAACAA AATGAAAGAA
    CTAGAACTTT TGTGTTCAAT GAAGGAAGTC TCCTTTGATG GAAATGATCT TGAAAACATG
    GTGCTATCTC TGAGGGAGAA GTTCCTACAA GAAGTGAATT CTCTTATTCA GAAACCCTCG
    CACCCACTGG CTAAAACGAA GACGTTAGTG AAGAGTCTAA TGAACCGAGC TGAGCTGTTG
    CTGCACGTCA CCATTGCAGC CCAGTCTGGC CTCACGAGAA GCATCTCAGG GACCCCCGCG
    GAAACACCGG CTTGTAAATC AGCCTCTGAA ACAAAGGTGA TCTCTCATGC TGTGCGTCAG
    CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC TCTGACCTGG AAATGATCGG TAATGAAGAT
    TTGGAATTTA CTAGAGCAAA TCAGAGGCGG CGCCACGTGA CCAGCCACCG CAGCAGCTCC
    TTCACACTCC TGCAGTCATT GGCCATAGAG GACAGCAGGG ACAAGCCCAC CTACAGCGTC
    CTGCTGGGGC AGCTGTTTGC TTTCATCGGC ACCAACCCTG ACCAAGCTGT GTCCAGCAGC
    AGCTTTCTTC TGGCCGCACA GACGAGATGG CGGCGGGGAA ACACCCGCAA GCAGGCGTTG
    GTGCACATGC GGGAGCTGCT GACGGCTGCC GTGCGAGTCG GGGGAGTGAC ACACCTTGTG
    GGTCCAGTGA CAATGGTCCT GCAGGGAGGA CCCAGAATTG AAGAACTGAC CTGTGGTGGG
    ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT GAGACCATGA CCTCTGTGGT GTCTTTGTGC
    GCACGTTACC CTATCGCATG TGCAAATAGC ATCGGACTCT TGTGCACTAT ACCTTATACC
    AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT GGCCTTGTCC AGCTCATGGA TCGACTATGC
    AGCTTGAGCA GTCAGACTGA GTCCAGCTCC AGTGAGAAAC AAACCAAGAA GCAGAAGGTG
    GCCACCATGG CCTGGGCAGC CTTCCAGGTG CTTGCCAACC GCTGCGTTGA ATGGGAAAAA
    GAAGAAGGCG GCTCCACAGA GGCTGTGCAC TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT
    CTCACGAATC ACCTTGCCCG AGCGACCGAG TGCTGTGGCA ACCAGGCTGC CGGGAACGAT
    GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT GATCTCTCGA GGAGTCATAT AGGTAAAGCC
    ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA CTCCTCTCCC TGCTACTTGA CCAACGCCCA
    TCCCCAAAGC TGGTTCTTAT CATTCTTCAG CTGTGCCGGG CGGCACTCCC GCTGATGAGC
    GTAGAAGACT GTGGGAACGT GGAGCTCCCA CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC
    AGCGAGCAGG AGGATCCCAG CGACCCAGCG TCCAAGATCG CCTCGCTGCT CTTGGCAAAG
    CTGGCAGACT ACGTGGTCCC AGGATGTCAG ACAGTCCTTT CTCCAACCGC TTCCGAACCT
    GACACCACAT TGACAAAAAC CAGCCCCAAG AATTCCTTAA AAGGCGATAA AGATCCTGGA
    GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC TCTATCTTCA TCCACAAGCG GGAAGACCAG
    TCGTCCCATG AAGTTCTGCA GCCATTACTA AGTAGCTCAG AAGGACGGCC CTTCCGACTT
    GGTACTGGTG CCAACATGGA GAAAGTTGTG AAAATGGACC GAGACATGAC GAAGGGTGGC
    TGCTGTGAGG TGATTACGGA AGAGGCGGCA GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA
    CAGTCAGGCC TCATTGTCAG TGTTGGGCCA CCCGTGGAGT CCCTCAACCC AGAGGCCGTG
    AGCGGGCTGT CCACAGGGGA CAAGAAGAAG ACTGCCCAAA CCTCCATTTG CCGAGAGAGA
    AACTCTGAAC TCGCCAGGAC CGACCCCGTC CGACCTTTCA TCAGTGGGCA CGTGGCAAAC
    AGCATGGCTG CTGAGGTGAT CGCCTTGCTT CATAGCTTGC TGATGGCACC TGAGTCAAAT
    GCTGCTCAGA TATGGACCAC GACAGCAGAG AAGGTTCTGT CTCGTGCACT GATGTACATT
    CCACAGTTGG GGAAATATGC AGAAAGCATT CTGGAAAATG GCAGCAGCAG TGGCAGAAAG
    CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG GCTGTGGCTG CACTCTGTGC ACTTGGAGGC
    TTCAAAGAGA CAATCAAGAT CGGGTCTGAG GTCCAGGTTT TAGGTAGAGG AATATCAGGA
    AGCATCGGAG TGGTGGCTTC TATCAACGAA CAGGAAGGTA TAGCTACGGT CAGATTCCCA
    CCCATAGACT GTAGAAAGAC TTCGCAAGCA TCAGACACAC TGACTATTCC ATTGTCTAGA
    CTTTGTGTTC CAAGATCAGA GGCATTGCCT CTTCATAAAC TGTCCATTAC TGAGAAGGTA
    GTACAGGCAG TCCAGTCCAT GTTGCTTCCT CAGGAGGGAA GTCTCTCTAT TCATACCTCA
    CTTCCTGCAG CAGGAGATGG GTCAGCTCCT GTGATGGCAG TCGTTCGGCT CCTGGCTGAA
    ATAAGAACAA GAGCATGCCT GGTCATGGCC CAGCTTTTAG AAGACAGCTT ATTTTGTGAA
    GAATTCATAC AGCAGTGTCC AGCAGCTGTT GAAGTTCTTA ACCTAGTAGC CCAGGAATGC
    AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA GTGCAGTGTG AACGGCTGAG GATGCTCTAC
    CGGGACTGTG CTCGGCCCCC GCCGCCTCCG CTGCAGGCTG ACCGGAGACA GCCCAAAGAG
    ATTACGTGGA GCCCCTCACG AGTGTTTCCA CCTGTCCGAG CCTGCATGTT CTCCTCGCAC
    CTCACTTCTG TCACCTTCCT AGCAGACCCC AGTGCAGGGG GCGGTCTGCC CAGGGGCACA
    TTTATCTACG CCACCTCACC ACTGCCCGTT CAGGCCCCGT CTTTCTACTG GGAAATTGAA
    ATTGTTTCCT ATGGAGATAC AGATGATGAT ACTGGACCAA TAGTTTCCTT TGGCTTTGCT
    ACAGAAGCAG AGAAAAGAGA TGGGGCATGG ACTAATCCAG TGGGCACCTG TCTTTTCCAT
    AACAATGGCC GAGCTGTGCA TTACAATGGC TCCAGTTTAT TACAGTGGAA GAGCGTTCGA
    TTAGACGTAA CTCTCTCTCC TGGTGACGTG GCAGGGATTG GCTGGGAGAG AACCGAGGGG
    ACGCCTCCTC CACCCGGGCA GCCTGCCAAG GGCCGTGTGT ACTTCACATA CTGTGGGCAG
    CGCCTCTCAC CGTATCTCGA AGATGTCTCG GGTGGGATGT GGCCGGTGGT TCATATTCAG
    AAAAAGAACA CCAAGACTCG CGCTAATTTT GGCTCCCGCC CATTTGCCTA CGCTGAAGGG
    CAAGCCCACC GCAATGCTGC CGACCTGTGC ACTGACCTGG CCGAGGAAAT TAGCGCTAAC
    TTCGAGGCCC TGCCCTTTGC CATGGCCTCT GACAGTGATA ACGATGCTGG CACCAGTATT
    GCGTCAGACC CGGGCACTCA TGGGCCACCA TGCCGAATTG CTGCCGTAGC CACTGCTCAG
    CAACAATATG ATAGTGATAC TTCCTGTCAT TATAAAGTGG AGCTCAGCTA TGAGAATTTC
    ATCACCTCTG GGCCAGACCC TCACCCACCC CCCATTGCAG ATGACGAAAG TGATGATGAT
    GATGACGATG ACATCCCACA GGAGGATCAC TATGCCCTGC TGGTGAAAGC CTGGGAGACC
    AAGGTTTTTC CTACCATCCG AAGACGGTTC CGCAACGAAG CAGAGAGGAA ATCAGGACTG
    GACCAGATAA AGGGTGCTTT ACAACTAGGC ATGGTGGATA TTGCCCGGCA GACAGTTGAA
    TTCCTCTACG AGGAGAATGG TGGCATCCCA AGAGACCTTT ATCTTCCCAC CATTGAAGAC
    ATTAAAGATG AAGCGAACAA GTTCACAATT GATAAAGTTC GAAAAGGTCT CACGGTAGTA
    ACACGCTCTC CAGACAGCAA TAACGTAGCC AGCAGCACTG TTGGAACTGC CCTGCCAAAA
    TTTGCTATCC GAGGGATGCT GAAAACCTTT GGGCTTCATG GAGTTGTCTT AGATGTTGAT
    TCAGTTAATG AACTAGTTCA GGTAGAAACG TACCTCCGAA GTGAAGGTGT GCTGGTCCGA
    TACTGGTACC CTATTGATAT GTTGGAAAGG CCCCCCGCAG GCTACAGAAG GACTGCCACC
    AATGGGCTCG TCACACTGGA CAATACCAAC CTTCAAATTC ACAGGGAGCT GCTGCGCTGC
    GAGGCCGCGC TGGCCAGGCT GTACTGCCGC ATGGCCCTGC TCAACATCTT CGCCCCCAAG
    CTGCCTCACC TGTTTACCCG CCTCTTCCAC ATCCCCGCCA TCCGGGACAT TACCCTGGAG
    CACCTGCAGC TGCTGTCCAA TCAGCTCCTC GCTCCTCCCC TCCCAGATGG CACCATCAGC
    TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG CAGCACTGTG TCCATTCCCA GAACTGTTCG
    GCCACAGACC TCTTCTACCA GGGCAGCTCC CAGACGGTGC GAGAGTGGCT CAACGTGGCC
    ATCACGCGGA CCCTGCACCA GGGCGAGGAA AGCCTTCTGG AGCTGACCAA ACAGATCTGC
    TCTTTCCTGC AGACAGCACC AGAACAGTTC CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC
    AAAGTCAACA TGGACGTCAA TTTCCCCGGG GCAGCTTTCG TCGTCGTATC TTGCAAAGAA
    AGTCAGTCTG GATTCCGCAA AGATTCCTCT CTGTACAAGG CACCATGGGC GCGTGTGCTG
    GTGTACGGGC TAGGCCACAA AGTGAAGCGA AATGGCCAGC TGAACCTCAT TGAGGCCGTC
    TGTTACCCGC GGGATGCGTC TCCGGCCAAC ACAGGGCTCA CACCACCTCC TACCACCAAC
    CAGTACCCTT CTGTGATCCT CTCCACAGAC AAAGTCCACA TCAAACTGGG GGTATCTCCC
    CCACCCGGAG CCGTGCTGGT GTTACACTCC CTGCCTCTCG AGTTCCCCCT GGCGATGGCC
    TTCACCGAGC AGCTACTGTC CTGGAAATCA GAGGATGGCG AAGGTAGAGC TGAAGATGAG
    CCTGACACCA TCCCGACGTC CGTCCTCCTG CAAGTGGTGG AGCTGCTTGG CAACTTCCTC
    TGGACCACAG ACATGGCGGC CTGCGTGAAG GAGCTGGTTT TCCACCTCCT GGCAGAGCTC
    CTGCGCACGG TGCACAGCCT AGAGCAGAGG AAGCACCCAG CCGGCCTGTC CTCATCCATC
    GCCCTCCAGC TGAACCCCTG CCTGGCCATG CTGATGGCCC TGCAGTCGGA GCTTCACAAG
    CTGTACGACG AGGAGACGCA GAGCTGGGTC TCGGGCAGTG CATGCGGAGG CTCAGGAGCC
    ACGGCGGCTG CTGACCAGGG CCGGTTCTCG ACGTATTTCC ACGCACTCAT GGAGGGCTGC
    CTGGCGGTGG CCGAGGTGAC CCTGCCCACC AACATGAGTG TCACCGCCAG CGGGGTGACG
    TCAGCAACTG CCCCAAACCT CAGCGACTCG TCCTCCTCCT CATCGTCCTC CCCAGGACAG
    ACTCCACAGA GCCCCAGCCT CCTGTCCAAG AGGAAGAAAG TCAAGATGAA GCGGGAAAAG
    GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA CGCTCTGTGG ACTCGGACCC CGCCGTGCTG
    AGCATCGGGG GCAGCAAGCC CGAGGACATG CTGTGGTTCC ATCGTGCTCT GACGTTGCTC
    ATCATCCTCC GCCACCTCAC CAGGAAGGAC CCACAGGGGC TAGGCGTGAC AAGCGACGCC
    ATCGCCGACG CCTGCCAGGC CCTGGTGGGC CCCACCGCCC ACAGCCGCTT GCTGGTGATC
    TCTGGGATCC CCACCCACCT GGACGAGGGC ATCGTCAGGG GTGCCATCCG CAAGGCCTGC
    AATGCCCATG GCGGGGTGTT CAAGGATGAG ATCTACATCC CCCTGCAGGA CGAAGACCCC
    AAGAAGCCAA AAGATAAGGC CGAGGGGGGT GATGGGAAAG CCGAGCCGGA AAAGACCCTC
    GGCTTCCCCA GCACGGACAG CTTGGAGGTC AGCACATCCA GCAGCCTGAC CCCCGCTATG
    AGCGTCAGCG CATCCGCGTC CACCAGCCAG GCTTCCATCT GCAGCTCCCA GGGCATCTCG
    CAGACAGTCA GTGACCTCTC TGCGGATCCG TTGCCTGCAG GCCTGGAGCT GCCCATCCCT
    GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC AGCCAGGAGA GCCTGGACAT TTCCCTGTGT
    AGCACCGGCA GCCTGGGCAG CCTGGGGAGC CTGGGCGAGC CACTGGACAA TGCAGAAACG
    GCCTCAGTGT CAGACATGGG CTCCATGTAC ACAGTCACGT CCCTGGACAA CCAGCCCCTT
    GCAGCGCGCC CCATCAAAGG CTTCGCGGTC GTGGAGATAA GGTCCCGAGC CAAAATTGAG
    AAAATTCGAG CAAGTTTATT TAACAACAAT GACTTGATAG GTTTGTCAAG CTTGGATGGT
    GAGGATGAAT TGATGGAAAT GTCAACGGAA GAGATCCTCA CGGTGTCTGT AGTGAATCAG
    AGTCTATTCG ATACGCAGGG GAGCCCAGGG TTAGAAGATT ATTTCAATGA TAAGTCAATT
    AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA GAGGTGCTGA CGGAGATATT TAAGAGCTGT
    GCCCACTCGG AGCAGATGCT GAGCCTGACG CCAGCAAAGC CCATCAAAGT GTCGGACATT
    TATCTTAGCA AAGAGCAGAT CAACTCCCAG ACACCGGGCA ACCTCCTCCA CCTTTTCTTC
    ACCAATGTCC GGCCCCCCAA AAAGGTGCTA GAGGACCAGC TCACCCAGAT CCTGAGGAAG
    TACGGTGTGC CGAAGCCCAA GTTTGACAAG AGCAAGTACA GCAAGGCGGG GAAGGAGCAG
    CACCCCGGGA AGGTGGTGAG CACCAAGCGG CCCATCACCA AGCCGCCCAC CAAGGACAAG
    GCAGTGCTCA ACAGCGTCAG CAGGACTGCC TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC
    AAGTCACCTG AAAAGAGCAA GCCAGATGAA AAGGACCCTG AAAAGTCACC CACCAAGAAA
    CAAGACGTCC CAGAGGAGAA GTACCTGACC TTAGAAGGAT TTCACAAGTT TGTGATCGAC
    CGAGCCAAGC AAGACATCCG CAGCGTCTGG AGAGCGATTT TATCCTGTGG CTACGATCTT
    CATTTTGAGA GGTGTGCCTG CATCGACGTC CGGCATGCGC ACAAGGCCTC CAGGAAGTGG
    ACCCTGGAGA TGGATGTGGC GCTCGTGCAG TACATCAACC GGCTGTGCCG CCACCTGGCC
    ATCACGCCCG CGCGTCTGCA TCCCCACGAG GTGTATCTGG ACCCTGCAGA CGCTGCTGAC
    CCCAGAGTGG CCTGTCTCTT GAACGTGCCC ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG
    CTGCAGTCCC TCAACACCAC GCTCGAGACC TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG
    ACTCCCATGT ACACGCACAG CATCGCCGCC CTGCTCAAGG AGGCCAAAGG GCTGATCTTT
    TATGACACAA AGGTGACCGT CATGAATCGG GTGCTAAATG CCACGGTGCA GAGGACGGCT
    GACCATGCAG CACCAGAAAT CACTCTGGAC CCGCTGGAGA TCGTGGGAGG GGAGATCAGA
    GCCTCTGAGA ACTCCTACTT CTGCCAGGCA GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC
    CAGCTTTGCG TCAAACTGGC GAGTGGCGGC GACCCTACCT ATGCCTTCAA CATCCGCTTC
    ACGGGAGAGG AGGTCCATGG CACCAGTGGC TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC
    AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC
    AAGAACAAGG GCAAGTACAT CCTGACCCCC AGCCCCATCA CCTATGGGGA GGAGCAGCTG
    CTGCACTTCC TGGGCCAGTT GCTAGGGATC GCCATCCGAG CAGATGTCCC CCTCCCCCTG
    GACCTCCTGC CCTCCTTCTG GAAGACACTG GTGGGCGAGC CCCTGGACCC TGACCAAGAC
    CTGCAGGAAG CAGACGTACT CACCTACAAT TACGTCAAGA AATTTGAGAG TATCAACGAC
    GAGACGGAGT TGGAGGCCCT GTGTGCTGAG ATCGCCTCCC AGCACCTGGC CACTGAGAGC
    CCCGAGGGGC CCAACAAGCC CTGCTGCCGG TTCACCTACC TGACCATGAC CGGCGAGGAG
    GTGGAGCTGT GCAGCCGTGG CCGGCACATC CCCGTGGCGT GGGAGAACAA GGACATCTAT
    GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT GAGCTACAGA ACGTGGAGTG TGTGACGGCC
    GTGCGCGCGG GCCTTGGGGC CATCATCCCC CTGCAGCTGC TCACCATGCT CAGCCCACTG
    GAGATGGAGC TGCGCACCTG CGGCCTCCCG TACATCAACC TCGAGTTCCT GAAGGCCCAC
    ACCATGTACC AGGTGGGGCT GATGGAGACC GACCAGCACA TCGAGTTCTT CTGGGGGGCA
    CTGGAGATGT TTGCCCAGGA GGAGCTGTGC AAGTTCATCA AGTTTGCCTG CAACCAGGAG
    CGCATCCCGT TCACCTGCCC CTGCAAAGAC GGGGGTCCTG ACACGGCCCA TGTGCCCCCA
    TACCCCATGA AGATTGCCCC CCCAGATGGC ACAGCAGGTT CCCCAGATTC TCGCTACATC
    CGAGTGGAGA CCTGTATGTT CATGATCAAG CTTCCCCAGT ACTCCTCGCT GGAAATCATG
    CTGGAGAAAC TACGTTGTGC CATTCACTAC CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812362.1
    CDS1026..12719
    Translation

    Target ORF information:

    RefSeq Version XM_010814060.2
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010814060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATTGGTG ATATACGCGG GGCCTAACAC AAACAGTAGG
    AAGGTTGCTG AGTATGGAGG CAATACACTG GGATATGGCA GCCGTAGTGT CTTAGGAACT
    GGTTGGCCGA AAGACTTGGT GAAGGTGGAA GGAGATACAG TCACCTTCTC CTTTGAAATG
    AGAAGTGGCC GTGAACACAA CACTCCCGAT AAGGCTATGT GGGGCTTTGC TTGCACAGTT
    CGCGCTCAGG AGTCTTCGGA GGATGTGTCA GGAGGCTTAC CCTTTCTGGT AGACCTGGCT
    TTAGGTCTAT CTGTGTTAGC TTGTTCCATG TTAAGAATCC TATACAACGG ACCAGAAATT
    ACCAAAGAAG AAGAAACCTG TCAGGAGCTG TTGCGGTCCA AACTTTTACA AAGGTGCCAG
    TGGCAGGTAG AGGCCAACGG TGTGATCTCC CCTGCCCTTA CTCCAAGCCC ATCTCCACTG
    CCTCTGACCA TCGAGGAAGA CAGAGAGTTC ACTTACCCGT CTGATGTCCT CGTGCCTCCT
    GTTGGAAACT ACTTTGACCT GCCCCGGATC AGGCTGCCTC CAGGAATCAT GATAAAGCTC
    AGGGAAATTT CTGGGCGTGC TAGACCACAG TTTAGACCAA GTATAAAAGA AGTAATTCAG
    CCAGATGTGA TGGAGGAAAT GGTGGTATCA TGTGTTATTA AGCACTTGAA CTTAGTTGAT
    GCACTGCAGT CCCTAATAAA TTTCCAGTAT CAAGAAGAAC ATGCTGAGGA ATATGATTTA
    CTGTGTAAAA TCATGGGAGA GACATTTAAG AAACTCAATG CCATGGAGAG ACAGCTGCAG
    AGTGTTGCAG AATTGGAACA GAAATGGCAG AGTGAAGTTG ATGATGCCAT GCAGGGGAAA
    CTGGAGAACA ACATGCCTTT CTTCTATGAT TACCATTTCA ATGAGAACAA AATGAAAGAA
    CTAGAACTTT TGTGTTCAAT GAAGGAAGTC TCCTTTGATG GAAATGATCT TGAAAACATG
    GTGCTATCTC TGAGGGAGAA GTTCCTACAA GAAGTGAATT CTCTTATTCA GAAACCCTCG
    CACCCACTGG CTAAAACGAA GACGTTAGTG AAGAGTCTAA TGAACCGAGC TGAGCTGTTG
    CTGCACGTCA CCATTGCAGC CCAGTCTGGC CTCACGAGAA GCATCTCAGG GACCCCCGCG
    GAAACACCGG CTTGTAAATC AGCCTCTGAA ACAAAGGTGA TCTCTCATGC TGTGCGTCAG
    CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC TCTGACCTGG AAATGATCGG TAATGAAGAT
    TTGGAATTTA CTAGAGCAAA TCAGAGGCGG CGCCACGTGA CCAGCCACCG CAGCAGCTCC
    TTCACACTCC TGCAGTCATT GGCCATAGAG GACAGCAGGG ACAAGCCCAC CTACAGCGTC
    CTGCTGGGGC AGCTGTTTGC TTTCATCGGC ACCAACCCTG ACCAAGCTGT GTCCAGCAGC
    AGCTTTCTTC TGGCCGCACA GACGAGATGG CGGCGGGGAA ACACCCGCAA GCAGGCGTTG
    GTGCACATGC GGGAGCTGCT GACGGCTGCC GTGCGAGTCG GGGGAGTGAC ACACCTTGTG
    GGTCCAGTGA CAATGGTCCT GCAGGGAGGA CCCAGAATTG AAGAACTGAC CTGTGGTGGG
    ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT GAGACCATGA CCTCTGTGGT GTCTTTGTGC
    GCACGTTACC CTATCGCATG TGCAAATAGC ATCGGACTCT TGTGCACTAT ACCTTATACC
    AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT GGCCTTGTCC AGCTCATGGA TCGACTATGC
    AGCTTGAGCA GTCAGACTGA GTCCAGCTCC AGTGAGAAAC AAACCAAGAA GCAGAAGGTG
    GCCACCATGG CCTGGGCAGC CTTCCAGGTG CTTGCCAACC GCTGCGTTGA ATGGGAAAAA
    GAAGAAGGCG GCTCCACAGA GGCTGTGCAC TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT
    CTCACGAATC ACCTTGCCCG AGCGACCGAG TGCTGTGGCA ACCAGGCTGC CGGGAACGAT
    GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT GATCTCTCGA GGAGTCATAT AGGTAAAGCC
    ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA CTCCTCTCCC TGCTACTTGA CCAACGCCCA
    TCCCCAAAGC TGGTTCTTAT CATTCTTCAG CTGTGCCGGG CGGCACTCCC GCTGATGAGC
    GTAGAAGACT GTGGGAACGT GGAGCTCCCA CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC
    AGCGAGCAGG AGGATCCCAG CGACCCAGCG TCCAAGATCG CCTCGCTGCT CTTGGCAAAG
    CTGGCAGACT ACGTGGTCCC AGGATGTCAG ACAGTCCTTT CTCCAACCGC TTCCGAACCT
    GACACCACAT TGACAAAAAC CAGCCCCAAG AATTCCTTAA AAGGCGATAA AGATCCTGGA
    GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC TCTATCTTCA TCCACAAGCG GGAAGACCAG
    TCGTCCCATG AAGTTCTGCA GCCATTACTA AGTAGCTCAG AAGGACGGCC CTTCCGACTT
    GGTACTGGTG CCAACATGGA GAAAGTTGTG AAAATGGACC GAGACATGAC GAAGGGTGGC
    TGCTGTGAGG TGATTACGGA AGAGGCGGCA GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA
    CAGTCAGGCC TCATTGTCAG TGTTGGGCCA CCCGTGGAGT CCCTCAACCC AGAGGCCGTG
    AGCGGGCTGT CCACAGGGGA CAAGAAGAAG ACTGCCCAAA CCTCCATTTG CCGAGAGAGA
    AACTCTGAAC TCGCCAGGAC CGACCCCGTC CGACCTTTCA TCAGTGGGCA CGTGGCAAAC
    AGCATGGCTG CTGAGGTGAT CGCCTTGCTT CATAGCTTGC TGATGGCACC TGAGTCAAAT
    GCTGCTCAGA TATGGACCAC GACAGCAGAG AAGGTTCTGT CTCGTGCACT GATGTACATT
    CCACAGTTGG GGAAATATGC AGAAAGCATT CTGGAAAATG GCAGCAGCAG TGGCAGAAAG
    CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG GCTGTGGCTG CACTCTGTGC ACTTGGAGGC
    TTCAAAGAGA CAATCAAGAT CGGGTCTGAG GTCCAGGTTT TAGGTAGAGG AATATCAGGA
    AGCATCGGAG TGGTGGCTTC TATCAACGAA CAGGAAGGTA TAGCTACGGT CAGATTCCCA
    CCCATAGACT GTAGAAAGAC TTCGCAAGCA TCAGACACAC TGACTATTCC ATTGTCTAGA
    CTTTGTGTTC CAAGATCAGA GGCATTGCCT CTTCATAAAC TGTCCATTAC TGAGAAGGTA
    GTACAGGCAG TCCAGTCCAT GTTGCTTCCT CAGGAGGGAA GTCTCTCTAT TCATACCTCA
    CTTCCTGCAG CAGGAGATGG GTCAGCTCCT GTGATGGCAG TCGTTCGGCT CCTGGCTGAA
    ATAAGAACAA GAGCATGCCT GGTCATGGCC CAGCTTTTAG AAGACAGCTT ATTTTGTGAA
    GAATTCATAC AGCAGTGTCC AGCAGCTGTT GAAGTTCTTA ACCTAGTAGC CCAGGAATGC
    AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA GTGCAGTGTG AACGGCTGAG GATGCTCTAC
    CGGGACTGTG CTCGGCCCCC GCCGCCTCCG CTGCAGGCTG ACCGGAGACA GCCCAAAGAG
    ATTACGTGGA GCCCCTCACG AGTGTTTCCA CCTGTCCGAG CCTGCATGTT CTCCTCGCAC
    CTCACTTCTG TCACCTTCCT AGCAGACCCC AGTGCAGGGG GCGGTCTGCC CAGGGGCACA
    TTTATCTACG CCACCTCACC ACTGCCCGTT CAGGCCCCGT CTTTCTACTG GGAAATTGAA
    ATTGTTTCCT ATGGAGATAC AGATGATGAT ACTGGACCAA TAGTTTCCTT TGGCTTTGCT
    ACAGAAGCAG AGAAAAGAGA TGGGGCATGG ACTAATCCAG TGGGCACCTG TCTTTTCCAT
    AACAATGGCC GAGCTGTGCA TTACAATGGC TCCAGTTTAT TACAGTGGAA GAGCGTTCGA
    TTAGACGTAA CTCTCTCTCC TGGTGACGTG GCAGGGATTG GCTGGGAGAG AACCGAGGGG
    ACGCCTCCTC CACCCGGGCA GCCTGCCAAG GGCCGTGTGT ACTTCACATA CTGTGGGCAG
    CGCCTCTCAC CGTATCTCGA AGATGTCTCG GGTGGGATGT GGCCGGTGGT TCATATTCAG
    AAAAAGAACA CCAAGACTCG CGCTAATTTT GGCTCCCGCC CATTTGCCTA CGCTGAAGGG
    CAAGCCCACC GCAATGCTGC CGACCTGTGC ACTGACCTGG CCGAGGAAAT TAGCGCTAAC
    TTCGAGGCCC TGCCCTTTGC CATGGCCTCT GACAGTGATA ACGATGCTGG CACCAGTATT
    GCGTCAGACC CGGGCACTCA TGGGCCACCA TGCCGAATTG CTGCCGTAGC CACTGCTCAG
    CAACAATATG ATAGTGATAC TTCCTGTCAT TATAAAGTGG AGCTCAGCTA TGAGAATTTC
    ATCACCTCTG GGCCAGACCC TCACCCACCC CCCATTGCAG ATGACGAAAG TGATGATGAT
    GATGACGATG ACATCCCACA GGAGGATCAC TATGCCCTGC TGGTGAAAGC CTGGGAGACC
    AAGGTTTTTC CTACCATCCG AAGACGGTTC CGCAACGAAG CAGAGAGGAA ATCAGGACTG
    GACCAGATAA AGGGTGCTTT ACAACTAGGC ATGGTGGATA TTGCCCGGCA GACAGTTGAA
    TTCCTCTACG AGGAGAATGG TGGCATCCCA AGAGACCTTT ATCTTCCCAC CATTGAAGAC
    ATTAAAGATG AAGCGAACAA GTTCACAATT GATAAAGTTC GAAAAGGTCT CACGGTAGTA
    ACACGCTCTC CAGACAGCAA TAACGTAGCC AGCAGCACTG TTGGAACTGC CCTGCCAAAA
    TTTGCTATCC GAGGGATGCT GAAAACCTTT GGGCTTCATG GAGTTGTCTT AGATGTTGAT
    TCAGTTAATG AACTAGTTCA GGTAGAAACG TACCTCCGAA GTGAAGGTGT GCTGGTCCGA
    TACTGGTACC CTATTGATAT GTTGGAAAGG CCCCCCGCAG GCTACAGAAG GACTGCCACC
    AATGGGCTCG TCACACTGGA CAATACCAAC CTTCAAATTC ACAGGGAGCT GCTGCGCTGC
    GAGGCCGCGC TGGCCAGGCT GTACTGCCGC ATGGCCCTGC TCAACATCTT CGCCCCCAAG
    CTGCCTCACC TGTTTACCCG CCTCTTCCAC ATCCCCGCCA TCCGGGACAT TACCCTGGAG
    CACCTGCAGC TGCTGTCCAA TCAGCTCCTC GCTCCTCCCC TCCCAGATGG CACCATCAGC
    TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG CAGCACTGTG TCCATTCCCA GAACTGTTCG
    GCCACAGACC TCTTCTACCA GGGCAGCTCC CAGACGGTGC GAGAGTGGCT CAACGTGGCC
    ATCACGCGGA CCCTGCACCA GGGCGAGGAA AGCCTTCTGG AGCTGACCAA ACAGATCTGC
    TCTTTCCTGC AGACAGCACC AGAACAGTTC CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC
    AAAGTCAACA TGGACGTCAA TTTCCCCGGG GCAGCTTTCG TCGTCGTATC TTGCAAAGAA
    AGTCAGTCTG GATTCCGCAA AGATTCCTCT CTGTACAAGG CACCATGGGC GCGTGTGCTG
    GTGTACGGGC TAGGCCACAA AGTGAAGCGA AATGGCCAGC TGAACCTCAT TGAGGCCGTC
    TGTTACCCGC GGGATGCGTC TCCGGCCAAC ACAGGGCTCA CACCACCTCC TACCACCAAC
    CAGTACCCTT CTGTGATCCT CTCCACAGAC AAAGTCCACA TCAAACTGGG GGTATCTCCC
    CCACCCGGAG CCGTGCTGGT GTTACACTCC CTGCCTCTCG AGTTCCCCCT GGCGATGGCC
    TTCACCGAGC AGCTACTGTC CTGGAAATCA GAGGATGGCG AAGGTAGAGC TGAAGATGAG
    CCTGACACCA TCCCGACGTC CGTCCTCCTG CAAGTGGTGG AGCTGCTTGG CAACTTCCTC
    TGGACCACAG ACATGGCGGC CTGCGTGAAG GAGCTGGTTT TCCACCTCCT GGCAGAGCTC
    CTGCGCACGG TGCACAGCCT AGAGCAGAGG AAGCACCCAG CCGGCCTGTC CTCATCCATC
    GCCCTCCAGC TGAACCCCTG CCTGGCCATG CTGATGGCCC TGCAGTCGGA GCTTCACAAG
    CTGTACGACG AGGAGACGCA GAGCTGGGTC TCGGGCAGTG CATGCGGAGG CTCAGGAGCC
    ACGGCGGCTG CTGACCAGGG CCGGTTCTCG ACGTATTTCC ACGCACTCAT GGAGGGCTGC
    CTGGCGGTGG CCGAGGTGAC CCTGCCCACC AACATGAGTG TCACCGCCAG CGGGGTGACG
    TCAGCAACTG CCCCAAACCT CAGCGACTCG TCCTCCTCCT CATCGTCCTC CCCAGGACAG
    ACTCCACAGA GCCCCAGCCT CCTGTCCAAG AGGAAGAAAG TCAAGATGAA GCGGGAAAAG
    GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA CGCTCTGTGG ACTCGGACCC CGCCGTGCTG
    AGCATCGGGG GCAGCAAGCC CGAGGACATG CTGTGGTTCC ATCGTGCTCT GACGTTGCTC
    ATCATCCTCC GCCACCTCAC CAGGAAGGAC CCACAGGGGC TAGGCGTGAC AAGCGACGCC
    ATCGCCGACG CCTGCCAGGC CCTGGTGGGC CCCACCGCCC ACAGCCGCTT GCTGGTGATC
    TCTGGGATCC CCACCCACCT GGACGAGGGC ATCGTCAGGG GTGCCATCCG CAAGGCCTGC
    AATGCCCATG GCGGGGTGTT CAAGGATGAG ATCTACATCC CCCTGCAGGA CGAAGACCCC
    AAGAAGCCAA AAGATAAGGC CGAGGGGGGT GATGGGAAAG CCGAGCCGGA AAAGACCCTC
    GGCTTCCCCA GCACGGACAG CTTGGAGGTC AGCACATCCA GCAGCCTGAC CCCCGCTATG
    AGCGTCAGCG CATCCGCGTC CACCAGCCAG GCTTCCATCT GCAGCTCCCA GGGCATCTCG
    CAGACAGTCA GTGACCTCTC TGCGGATCCG TTGCCTGCAG GCCTGGAGCT GCCCATCCCT
    GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC AGCCAGGAGA GCCTGGACAT TTCCCTGTGT
    AGCACCGGCA GCCTGGGCAG CCTGGGGAGC CTGGGCGAGC CACTGGACAA TGCAGAAACG
    GCCTCAGTGT CAGACATGGG CTCCATGTAC ACAGTCACGT CCCTGGACAA CCAGCCCCTT
    GCAGCGCGCC CCATCAAAGG CTTCGCGGTC GTGGAGATAA GGTCCCGAGC CAAAATTGAG
    AAAATTCGAG CAAGTTTATT TAACAACAAT GACTTGATAG GTTTGTCAAG CTTGGATGGT
    GAGGATGAAT TGATGGAAAT GTCAACGGAA GAGATCCTCA CGGTGTCTGT AGTGAATCAG
    AGTCTATTCG ATACGCAGGG GAGCCCAGGG TTAGAAGATT ATTTCAATGA TAAGTCAATT
    AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA GAGGTGCTGA CGGAGATATT TAAGAGCTGT
    GCCCACTCGG AGCAGATGCT GAGCCTGACG CCAGCAAAGC CCATCAAAGT GTCGGACATT
    TATCTTAGCA AAGAGCAGAT CAACTCCCAG ACACCGGGCA ACCTCCTCCA CCTTTTCTTC
    ACCAATGTCC GGCCCCCCAA AAAGGTGCTA GAGGACCAGC TCACCCAGAT CCTGAGGAAG
    TACGGTGTGC CGAAGCCCAA GTTTGACAAG AGCAAGTACA GCAAGGCGGG GAAGGAGCAG
    CACCCCGGGA AGGTGGTGAG CACCAAGCGG CCCATCACCA AGCCGCCCAC CAAGGACAAG
    GCAGTGCTCA ACAGCGTCAG CAGGACTGCC TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC
    AAGTCACCTG AAAAGAGCAA GCCAGATGAA AAGGACCCTG AAAAGTCACC CACCAAGAAA
    CAAGACGTCC CAGAGGAGAA GTACCTGACC TTAGAAGGAT TTCACAAGTT TGTGATCGAC
    CGAGCCAAGC AAGACATCCG CAGCGTCTGG AGAGCGATTT TATCCTGTGG CTACGATCTT
    CATTTTGAGA GGTGTGCCTG CATCGACGTC CGGCATGCGC ACAAGGCCTC CAGGAAGTGG
    ACCCTGGAGA TGGATGTGGC GCTCGTGCAG TACATCAACC GGCTGTGCCG CCACCTGGCC
    ATCACGCCCG CGCGTCTGCA TCCCCACGAG GTGTATCTGG ACCCTGCAGA CGCTGCTGAC
    CCCAGAGTGG CCTGTCTCTT GAACGTGCCC ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG
    CTGCAGTCCC TCAACACCAC GCTCGAGACC TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG
    ACTCCCATGT ACACGCACAG CATCGCCGCC CTGCTCAAGG AGGCCAAAGG GCTGATCTTT
    TATGACACAA AGGTGACCGT CATGAATCGG GTGCTAAATG CCACGGTGCA GAGGACGGCT
    GACCATGCAG CACCAGAAAT CACTCTGGAC CCGCTGGAGA TCGTGGGAGG GGAGATCAGA
    GCCTCTGAGA ACTCCTACTT CTGCCAGGCA GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC
    CAGCTTTGCG TCAAACTGGC GAGTGGCGGC GACCCTACCT ATGCCTTCAA CATCCGCTTC
    ACGGGAGAGG AGGTCCATGG CACCAGTGGC TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC
    AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC
    AAGAACAAGG GCAAGTACAT CCTGACCCCC AGCCCCATCA CCTATGGGGA GGAGCAGCTG
    CTGCACTTCC TGGGCCAGTT GCTAGGGATC GCCATCCGAG CAGATGTCCC CCTCCCCCTG
    GACCTCCTGC CCTCCTTCTG GAAGACACTG GTGGGCGAGC CCCTGGACCC TGACCAAGAC
    CTGCAGGAAG CAGACGTACT CACCTACAAT TACGTCAAGA AATTTGAGAG TATCAACGAC
    GAGACGGAGT TGGAGGCCCT GTGTGCTGAG ATCGCCTCCC AGCACCTGGC CACTGAGAGC
    CCCGAGGGGC CCAACAAGCC CTGCTGCCGG TTCACCTACC TGACCATGAC CGGCGAGGAG
    GTGGAGCTGT GCAGCCGTGG CCGGCACATC CCCGTGGCGT GGGAGAACAA GGACATCTAT
    GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT GAGCTACAGA ACGTGGAGTG TGTGACGGCC
    GTGCGCGCGG GCCTTGGGGC CATCATCCCC CTGCAGCTGC TCACCATGCT CAGCCCACTG
    GAGATGGAGC TGCGCACCTG CGGCCTCCCG TACATCAACC TCGAGTTCCT GAAGGCCCAC
    ACCATGTACC AGGTGGGGCT GATGGAGACC GACCAGCACA TCGAGTTCTT CTGGGGGGCA
    CTGGAGATGT TTGCCCAGGA GGAGCTGTGC AAGTTCATCA AGTTTGCCTG CAACCAGGAG
    CGCATCCCGT TCACCTGCCC CTGCAAAGAC GGGGGTCCTG ACACGGCCCA TGTGCCCCCA
    TACCCCATGA AGATTGCCCC CCCAGATGGC ACAGCAGGTT CCCCAGATTC TCGCTACATC
    CGAGTGGAGA CCTGTATGTT CATGATCAAG CTTCCCCAGT ACTCCTCGCT GGAAATCATG
    CTGGAGAAAC TACGTTGTGC CATTCACTAC CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa52732
    Clone ID Related Accession (Same CDS sequence) XM_010814060.2 , XM_015467165.1
    Accession Version XM_015467165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11694bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    601
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    721
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    841
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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
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    2641
    2701
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    4141
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    4261
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    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
    5041
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    5281
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    5521
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    5701
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    5881
    5941
    6001
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    6421
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    9301
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATTGGTG ATATACGCGG GGCCTAACAC AAACAGTAGG
    AAGGTTGCTG AGTATGGAGG CAATACACTG GGATATGGCA GCCGTAGTGT CTTAGGAACT
    GGTTGGCCGA AAGACTTGGT GAAGGTGGAA GGAGATACAG TCACCTTCTC CTTTGAAATG
    AGAAGTGGCC GTGAACACAA CACTCCCGAT AAGGCTATGT GGGGCTTTGC TTGCACAGTT
    CGCGCTCAGG AGTCTTCGGA GGATGTGTCA GGAGGCTTAC CCTTTCTGGT AGACCTGGCT
    TTAGGTCTAT CTGTGTTAGC TTGTTCCATG TTAAGAATCC TATACAACGG ACCAGAAATT
    ACCAAAGAAG AAGAAACCTG TCAGGAGCTG TTGCGGTCCA AACTTTTACA AAGGTGCCAG
    TGGCAGGTAG AGGCCAACGG TGTGATCTCC CCTGCCCTTA CTCCAAGCCC ATCTCCACTG
    CCTCTGACCA TCGAGGAAGA CAGAGAGTTC ACTTACCCGT CTGATGTCCT CGTGCCTCCT
    GTTGGAAACT ACTTTGACCT GCCCCGGATC AGGCTGCCTC CAGGAATCAT GATAAAGCTC
    AGGGAAATTT CTGGGCGTGC TAGACCACAG TTTAGACCAA GTATAAAAGA AGTAATTCAG
    CCAGATGTGA TGGAGGAAAT GGTGGTATCA TGTGTTATTA AGCACTTGAA CTTAGTTGAT
    GCACTGCAGT CCCTAATAAA TTTCCAGTAT CAAGAAGAAC ATGCTGAGGA ATATGATTTA
    CTGTGTAAAA TCATGGGAGA GACATTTAAG AAACTCAATG CCATGGAGAG ACAGCTGCAG
    AGTGTTGCAG AATTGGAACA GAAATGGCAG AGTGAAGTTG ATGATGCCAT GCAGGGGAAA
    CTGGAGAACA ACATGCCTTT CTTCTATGAT TACCATTTCA ATGAGAACAA AATGAAAGAA
    CTAGAACTTT TGTGTTCAAT GAAGGAAGTC TCCTTTGATG GAAATGATCT TGAAAACATG
    GTGCTATCTC TGAGGGAGAA GTTCCTACAA GAAGTGAATT CTCTTATTCA GAAACCCTCG
    CACCCACTGG CTAAAACGAA GACGTTAGTG AAGAGTCTAA TGAACCGAGC TGAGCTGTTG
    CTGCACGTCA CCATTGCAGC CCAGTCTGGC CTCACGAGAA GCATCTCAGG GACCCCCGCG
    GAAACACCGG CTTGTAAATC AGCCTCTGAA ACAAAGGTGA TCTCTCATGC TGTGCGTCAG
    CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC TCTGACCTGG AAATGATCGG TAATGAAGAT
    TTGGAATTTA CTAGAGCAAA TCAGAGGCGG CGCCACGTGA CCAGCCACCG CAGCAGCTCC
    TTCACACTCC TGCAGTCATT GGCCATAGAG GACAGCAGGG ACAAGCCCAC CTACAGCGTC
    CTGCTGGGGC AGCTGTTTGC TTTCATCGGC ACCAACCCTG ACCAAGCTGT GTCCAGCAGC
    AGCTTTCTTC TGGCCGCACA GACGAGATGG CGGCGGGGAA ACACCCGCAA GCAGGCGTTG
    GTGCACATGC GGGAGCTGCT GACGGCTGCC GTGCGAGTCG GGGGAGTGAC ACACCTTGTG
    GGTCCAGTGA CAATGGTCCT GCAGGGAGGA CCCAGAATTG AAGAACTGAC CTGTGGTGGG
    ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT GAGACCATGA CCTCTGTGGT GTCTTTGTGC
    GCACGTTACC CTATCGCATG TGCAAATAGC ATCGGACTCT TGTGCACTAT ACCTTATACC
    AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT GGCCTTGTCC AGCTCATGGA TCGACTATGC
    AGCTTGAGCA GTCAGACTGA GTCCAGCTCC AGTGAGAAAC AAACCAAGAA GCAGAAGGTG
    GCCACCATGG CCTGGGCAGC CTTCCAGGTG CTTGCCAACC GCTGCGTTGA ATGGGAAAAA
    GAAGAAGGCG GCTCCACAGA GGCTGTGCAC TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT
    CTCACGAATC ACCTTGCCCG AGCGACCGAG TGCTGTGGCA ACCAGGCTGC CGGGAACGAT
    GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT GATCTCTCGA GGAGTCATAT AGGTAAAGCC
    ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA CTCCTCTCCC TGCTACTTGA CCAACGCCCA
    TCCCCAAAGC TGGTTCTTAT CATTCTTCAG CTGTGCCGGG CGGCACTCCC GCTGATGAGC
    GTAGAAGACT GTGGGAACGT GGAGCTCCCA CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC
    AGCGAGCAGG AGGATCCCAG CGACCCAGCG TCCAAGATCG CCTCGCTGCT CTTGGCAAAG
    CTGGCAGACT ACGTGGTCCC AGGATGTCAG ACAGTCCTTT CTCCAACCGC TTCCGAACCT
    GACACCACAT TGACAAAAAC CAGCCCCAAG AATTCCTTAA AAGGCGATAA AGATCCTGGA
    GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC TCTATCTTCA TCCACAAGCG GGAAGACCAG
    TCGTCCCATG AAGTTCTGCA GCCATTACTA AGTAGCTCAG AAGGACGGCC CTTCCGACTT
    GGTACTGGTG CCAACATGGA GAAAGTTGTG AAAATGGACC GAGACATGAC GAAGGGTGGC
    TGCTGTGAGG TGATTACGGA AGAGGCGGCA GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA
    CAGTCAGGCC TCATTGTCAG TGTTGGGCCA CCCGTGGAGT CCCTCAACCC AGAGGCCGTG
    AGCGGGCTGT CCACAGGGGA CAAGAAGAAG ACTGCCCAAA CCTCCATTTG CCGAGAGAGA
    AACTCTGAAC TCGCCAGGAC CGACCCCGTC CGACCTTTCA TCAGTGGGCA CGTGGCAAAC
    AGCATGGCTG CTGAGGTGAT CGCCTTGCTT CATAGCTTGC TGATGGCACC TGAGTCAAAT
    GCTGCTCAGA TATGGACCAC GACAGCAGAG AAGGTTCTGT CTCGTGCACT GATGTACATT
    CCACAGTTGG GGAAATATGC AGAAAGCATT CTGGAAAATG GCAGCAGCAG TGGCAGAAAG
    CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG GCTGTGGCTG CACTCTGTGC ACTTGGAGGC
    TTCAAAGAGA CAATCAAGAT CGGGTCTGAG GTCCAGGTTT TAGGTAGAGG AATATCAGGA
    AGCATCGGAG TGGTGGCTTC TATCAACGAA CAGGAAGGTA TAGCTACGGT CAGATTCCCA
    CCCATAGACT GTAGAAAGAC TTCGCAAGCA TCAGACACAC TGACTATTCC ATTGTCTAGA
    CTTTGTGTTC CAAGATCAGA GGCATTGCCT CTTCATAAAC TGTCCATTAC TGAGAAGGTA
    GTACAGGCAG TCCAGTCCAT GTTGCTTCCT CAGGAGGGAA GTCTCTCTAT TCATACCTCA
    CTTCCTGCAG CAGGAGATGG GTCAGCTCCT GTGATGGCAG TCGTTCGGCT CCTGGCTGAA
    ATAAGAACAA GAGCATGCCT GGTCATGGCC CAGCTTTTAG AAGACAGCTT ATTTTGTGAA
    GAATTCATAC AGCAGTGTCC AGCAGCTGTT GAAGTTCTTA ACCTAGTAGC CCAGGAATGC
    AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA GTGCAGTGTG AACGGCTGAG GATGCTCTAC
    CGGGACTGTG CTCGGCCCCC GCCGCCTCCG CTGCAGGCTG ACCGGAGACA GCCCAAAGAG
    ATTACGTGGA GCCCCTCACG AGTGTTTCCA CCTGTCCGAG CCTGCATGTT CTCCTCGCAC
    CTCACTTCTG TCACCTTCCT AGCAGACCCC AGTGCAGGGG GCGGTCTGCC CAGGGGCACA
    TTTATCTACG CCACCTCACC ACTGCCCGTT CAGGCCCCGT CTTTCTACTG GGAAATTGAA
    ATTGTTTCCT ATGGAGATAC AGATGATGAT ACTGGACCAA TAGTTTCCTT TGGCTTTGCT
    ACAGAAGCAG AGAAAAGAGA TGGGGCATGG ACTAATCCAG TGGGCACCTG TCTTTTCCAT
    AACAATGGCC GAGCTGTGCA TTACAATGGC TCCAGTTTAT TACAGTGGAA GAGCGTTCGA
    TTAGACGTAA CTCTCTCTCC TGGTGACGTG GCAGGGATTG GCTGGGAGAG AACCGAGGGG
    ACGCCTCCTC CACCCGGGCA GCCTGCCAAG GGCCGTGTGT ACTTCACATA CTGTGGGCAG
    CGCCTCTCAC CGTATCTCGA AGATGTCTCG GGTGGGATGT GGCCGGTGGT TCATATTCAG
    AAAAAGAACA CCAAGACTCG CGCTAATTTT GGCTCCCGCC CATTTGCCTA CGCTGAAGGG
    CAAGCCCACC GCAATGCTGC CGACCTGTGC ACTGACCTGG CCGAGGAAAT TAGCGCTAAC
    TTCGAGGCCC TGCCCTTTGC CATGGCCTCT GACAGTGATA ACGATGCTGG CACCAGTATT
    GCGTCAGACC CGGGCACTCA TGGGCCACCA TGCCGAATTG CTGCCGTAGC CACTGCTCAG
    CAACAATATG ATAGTGATAC TTCCTGTCAT TATAAAGTGG AGCTCAGCTA TGAGAATTTC
    ATCACCTCTG GGCCAGACCC TCACCCACCC CCCATTGCAG ATGACGAAAG TGATGATGAT
    GATGACGATG ACATCCCACA GGAGGATCAC TATGCCCTGC TGGTGAAAGC CTGGGAGACC
    AAGGTTTTTC CTACCATCCG AAGACGGTTC CGCAACGAAG CAGAGAGGAA ATCAGGACTG
    GACCAGATAA AGGGTGCTTT ACAACTAGGC ATGGTGGATA TTGCCCGGCA GACAGTTGAA
    TTCCTCTACG AGGAGAATGG TGGCATCCCA AGAGACCTTT ATCTTCCCAC CATTGAAGAC
    ATTAAAGATG AAGCGAACAA GTTCACAATT GATAAAGTTC GAAAAGGTCT CACGGTAGTA
    ACACGCTCTC CAGACAGCAA TAACGTAGCC AGCAGCACTG TTGGAACTGC CCTGCCAAAA
    TTTGCTATCC GAGGGATGCT GAAAACCTTT GGGCTTCATG GAGTTGTCTT AGATGTTGAT
    TCAGTTAATG AACTAGTTCA GGTAGAAACG TACCTCCGAA GTGAAGGTGT GCTGGTCCGA
    TACTGGTACC CTATTGATAT GTTGGAAAGG CCCCCCGCAG GCTACAGAAG GACTGCCACC
    AATGGGCTCG TCACACTGGA CAATACCAAC CTTCAAATTC ACAGGGAGCT GCTGCGCTGC
    GAGGCCGCGC TGGCCAGGCT GTACTGCCGC ATGGCCCTGC TCAACATCTT CGCCCCCAAG
    CTGCCTCACC TGTTTACCCG CCTCTTCCAC ATCCCCGCCA TCCGGGACAT TACCCTGGAG
    CACCTGCAGC TGCTGTCCAA TCAGCTCCTC GCTCCTCCCC TCCCAGATGG CACCATCAGC
    TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG CAGCACTGTG TCCATTCCCA GAACTGTTCG
    GCCACAGACC TCTTCTACCA GGGCAGCTCC CAGACGGTGC GAGAGTGGCT CAACGTGGCC
    ATCACGCGGA CCCTGCACCA GGGCGAGGAA AGCCTTCTGG AGCTGACCAA ACAGATCTGC
    TCTTTCCTGC AGACAGCACC AGAACAGTTC CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC
    AAAGTCAACA TGGACGTCAA TTTCCCCGGG GCAGCTTTCG TCGTCGTATC TTGCAAAGAA
    AGTCAGTCTG GATTCCGCAA AGATTCCTCT CTGTACAAGG CACCATGGGC GCGTGTGCTG
    GTGTACGGGC TAGGCCACAA AGTGAAGCGA AATGGCCAGC TGAACCTCAT TGAGGCCGTC
    TGTTACCCGC GGGATGCGTC TCCGGCCAAC ACAGGGCTCA CACCACCTCC TACCACCAAC
    CAGTACCCTT CTGTGATCCT CTCCACAGAC AAAGTCCACA TCAAACTGGG GGTATCTCCC
    CCACCCGGAG CCGTGCTGGT GTTACACTCC CTGCCTCTCG AGTTCCCCCT GGCGATGGCC
    TTCACCGAGC AGCTACTGTC CTGGAAATCA GAGGATGGCG AAGGTAGAGC TGAAGATGAG
    CCTGACACCA TCCCGACGTC CGTCCTCCTG CAAGTGGTGG AGCTGCTTGG CAACTTCCTC
    TGGACCACAG ACATGGCGGC CTGCGTGAAG GAGCTGGTTT TCCACCTCCT GGCAGAGCTC
    CTGCGCACGG TGCACAGCCT AGAGCAGAGG AAGCACCCAG CCGGCCTGTC CTCATCCATC
    GCCCTCCAGC TGAACCCCTG CCTGGCCATG CTGATGGCCC TGCAGTCGGA GCTTCACAAG
    CTGTACGACG AGGAGACGCA GAGCTGGGTC TCGGGCAGTG CATGCGGAGG CTCAGGAGCC
    ACGGCGGCTG CTGACCAGGG CCGGTTCTCG ACGTATTTCC ACGCACTCAT GGAGGGCTGC
    CTGGCGGTGG CCGAGGTGAC CCTGCCCACC AACATGAGTG TCACCGCCAG CGGGGTGACG
    TCAGCAACTG CCCCAAACCT CAGCGACTCG TCCTCCTCCT CATCGTCCTC CCCAGGACAG
    ACTCCACAGA GCCCCAGCCT CCTGTCCAAG AGGAAGAAAG TCAAGATGAA GCGGGAAAAG
    GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA CGCTCTGTGG ACTCGGACCC CGCCGTGCTG
    AGCATCGGGG GCAGCAAGCC CGAGGACATG CTGTGGTTCC ATCGTGCTCT GACGTTGCTC
    ATCATCCTCC GCCACCTCAC CAGGAAGGAC CCACAGGGGC TAGGCGTGAC AAGCGACGCC
    ATCGCCGACG CCTGCCAGGC CCTGGTGGGC CCCACCGCCC ACAGCCGCTT GCTGGTGATC
    TCTGGGATCC CCACCCACCT GGACGAGGGC ATCGTCAGGG GTGCCATCCG CAAGGCCTGC
    AATGCCCATG GCGGGGTGTT CAAGGATGAG ATCTACATCC CCCTGCAGGA CGAAGACCCC
    AAGAAGCCAA AAGATAAGGC CGAGGGGGGT GATGGGAAAG CCGAGCCGGA AAAGACCCTC
    GGCTTCCCCA GCACGGACAG CTTGGAGGTC AGCACATCCA GCAGCCTGAC CCCCGCTATG
    AGCGTCAGCG CATCCGCGTC CACCAGCCAG GCTTCCATCT GCAGCTCCCA GGGCATCTCG
    CAGACAGTCA GTGACCTCTC TGCGGATCCG TTGCCTGCAG GCCTGGAGCT GCCCATCCCT
    GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC AGCCAGGAGA GCCTGGACAT TTCCCTGTGT
    AGCACCGGCA GCCTGGGCAG CCTGGGGAGC CTGGGCGAGC CACTGGACAA TGCAGAAACG
    GCCTCAGTGT CAGACATGGG CTCCATGTAC ACAGTCACGT CCCTGGACAA CCAGCCCCTT
    GCAGCGCGCC CCATCAAAGG CTTCGCGGTC GTGGAGATAA GGTCCCGAGC CAAAATTGAG
    AAAATTCGAG CAAGTTTATT TAACAACAAT GACTTGATAG GTTTGTCAAG CTTGGATGGT
    GAGGATGAAT TGATGGAAAT GTCAACGGAA GAGATCCTCA CGGTGTCTGT AGTGAATCAG
    AGTCTATTCG ATACGCAGGG GAGCCCAGGG TTAGAAGATT ATTTCAATGA TAAGTCAATT
    AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA GAGGTGCTGA CGGAGATATT TAAGAGCTGT
    GCCCACTCGG AGCAGATGCT GAGCCTGACG CCAGCAAAGC CCATCAAAGT GTCGGACATT
    TATCTTAGCA AAGAGCAGAT CAACTCCCAG ACACCGGGCA ACCTCCTCCA CCTTTTCTTC
    ACCAATGTCC GGCCCCCCAA AAAGGTGCTA GAGGACCAGC TCACCCAGAT CCTGAGGAAG
    TACGGTGTGC CGAAGCCCAA GTTTGACAAG AGCAAGTACA GCAAGGCGGG GAAGGAGCAG
    CACCCCGGGA AGGTGGTGAG CACCAAGCGG CCCATCACCA AGCCGCCCAC CAAGGACAAG
    GCAGTGCTCA ACAGCGTCAG CAGGACTGCC TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC
    AAGTCACCTG AAAAGAGCAA GCCAGATGAA AAGGACCCTG AAAAGTCACC CACCAAGAAA
    CAAGACGTCC CAGAGGAGAA GTACCTGACC TTAGAAGGAT TTCACAAGTT TGTGATCGAC
    CGAGCCAAGC AAGACATCCG CAGCGTCTGG AGAGCGATTT TATCCTGTGG CTACGATCTT
    CATTTTGAGA GGTGTGCCTG CATCGACGTC CGGCATGCGC ACAAGGCCTC CAGGAAGTGG
    ACCCTGGAGA TGGATGTGGC GCTCGTGCAG TACATCAACC GGCTGTGCCG CCACCTGGCC
    ATCACGCCCG CGCGTCTGCA TCCCCACGAG GTGTATCTGG ACCCTGCAGA CGCTGCTGAC
    CCCAGAGTGG CCTGTCTCTT GAACGTGCCC ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG
    CTGCAGTCCC TCAACACCAC GCTCGAGACC TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG
    ACTCCCATGT ACACGCACAG CATCGCCGCC CTGCTCAAGG AGGCCAAAGG GCTGATCTTT
    TATGACACAA AGGTGACCGT CATGAATCGG GTGCTAAATG CCACGGTGCA GAGGACGGCT
    GACCATGCAG CACCAGAAAT CACTCTGGAC CCGCTGGAGA TCGTGGGAGG GGAGATCAGA
    GCCTCTGAGA ACTCCTACTT CTGCCAGGCA GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC
    CAGCTTTGCG TCAAACTGGC GAGTGGCGGC GACCCTACCT ATGCCTTCAA CATCCGCTTC
    ACGGGAGAGG AGGTCCATGG CACCAGTGGC TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC
    AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC
    AAGAACAAGG GCAAGTACAT CCTGACCCCC AGCCCCATCA CCTATGGGGA GGAGCAGCTG
    CTGCACTTCC TGGGCCAGTT GCTAGGGATC GCCATCCGAG CAGATGTCCC CCTCCCCCTG
    GACCTCCTGC CCTCCTTCTG GAAGACACTG GTGGGCGAGC CCCTGGACCC TGACCAAGAC
    CTGCAGGAAG CAGACGTACT CACCTACAAT TACGTCAAGA AATTTGAGAG TATCAACGAC
    GAGACGGAGT TGGAGGCCCT GTGTGCTGAG ATCGCCTCCC AGCACCTGGC CACTGAGAGC
    CCCGAGGGGC CCAACAAGCC CTGCTGCCGG TTCACCTACC TGACCATGAC CGGCGAGGAG
    GTGGAGCTGT GCAGCCGTGG CCGGCACATC CCCGTGGCGT GGGAGAACAA GGACATCTAT
    GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT GAGCTACAGA ACGTGGAGTG TGTGACGGCC
    GTGCGCGCGG GCCTTGGGGC CATCATCCCC CTGCAGCTGC TCACCATGCT CAGCCCACTG
    GAGATGGAGC TGCGCACCTG CGGCCTCCCG TACATCAACC TCGAGTTCCT GAAGGCCCAC
    ACCATGTACC AGGTGGGGCT GATGGAGACC GACCAGCACA TCGAGTTCTT CTGGGGGGCA
    CTGGAGATGT TTGCCCAGGA GGAGCTGTGC AAGTTCATCA AGTTTGCCTG CAACCAGGAG
    CGCATCCCGT TCACCTGCCC CTGCAAAGAC GGGGGTCCTG ACACGGCCCA TGTGCCCCCA
    TACCCCATGA AGATTGCCCC CCCAGATGGC ACAGCAGGTT CCCCAGATTC TCGCTACATC
    CGAGTGGAGA CCTGTATGTT CATGATCAAG CTTCCCCAGT ACTCCTCGCT GGAAATCATG
    CTGGAGAAAC TACGTTGTGC CATTCACTAC CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322651.1
    CDS1026..12719
    Translation

    Target ORF information:

    RefSeq Version XM_015467165.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGAGTT AAAGGTCAGT
    ATTTTGGCTC TTGCTACCCA AATCCTGACT GGATGTGATG AAGTGTTGGA AATGCTACAG
    CAGGTCACAA CTGCCCTCAT AAATAGTGAC ATACCCGACC GGGAGCAGAG GTTAAAAGGC
    TTGGAACAAG TTACTAAGGC TACTATGCTT GGTCACCTTC TCCCAGTGTT ACTCACTTCC
    CTGATGCATC CAAATTTACA AACTTTGACC ATGGCCGATG CCCTGATGCC TCAGCTAGTG
    CAGCTGGTAC TCTATACCAG TCAGACGGCT TTGCTGCTTA AAACCCAGTG TCCTGTTTTT
    GCTGAGGTGG GCTGTTCCCC ATGTGGTGCA TCAGACCAGA AGGGCAGGCT TTTGCCGGAT
    GAGAGAATGC TGGAAGAGAA GGAGGAGCCA GGGTTTCTCA CTGGTTTAAA GATTCCTGCT
    CCATGGGCTG CTGGGAAGAC TGTGGAAACA GTCCACCCTG TCAGAGACAA CTATAAATTT
    AAAGAAACTG TCCACATCCC CGGAGCTCGC TGCCTGTACC TTAGATTTGA TAGCAGATGC
    TCTTCGCAAT ATGACTACGA CAAATTGGTG ATATACGCGG GGCCTAACAC AAACAGTAGG
    AAGGTTGCTG AGTATGGAGG CAATACACTG GGATATGGCA GCCGTAGTGT CTTAGGAACT
    GGTTGGCCGA AAGACTTGGT GAAGGTGGAA GGAGATACAG TCACCTTCTC CTTTGAAATG
    AGAAGTGGCC GTGAACACAA CACTCCCGAT AAGGCTATGT GGGGCTTTGC TTGCACAGTT
    CGCGCTCAGG AGTCTTCGGA GGATGTGTCA GGAGGCTTAC CCTTTCTGGT AGACCTGGCT
    TTAGGTCTAT CTGTGTTAGC TTGTTCCATG TTAAGAATCC TATACAACGG ACCAGAAATT
    ACCAAAGAAG AAGAAACCTG TCAGGAGCTG TTGCGGTCCA AACTTTTACA AAGGTGCCAG
    TGGCAGGTAG AGGCCAACGG TGTGATCTCC CCTGCCCTTA CTCCAAGCCC ATCTCCACTG
    CCTCTGACCA TCGAGGAAGA CAGAGAGTTC ACTTACCCGT CTGATGTCCT CGTGCCTCCT
    GTTGGAAACT ACTTTGACCT GCCCCGGATC AGGCTGCCTC CAGGAATCAT GATAAAGCTC
    AGGGAAATTT CTGGGCGTGC TAGACCACAG TTTAGACCAA GTATAAAAGA AGTAATTCAG
    CCAGATGTGA TGGAGGAAAT GGTGGTATCA TGTGTTATTA AGCACTTGAA CTTAGTTGAT
    GCACTGCAGT CCCTAATAAA TTTCCAGTAT CAAGAAGAAC ATGCTGAGGA ATATGATTTA
    CTGTGTAAAA TCATGGGAGA GACATTTAAG AAACTCAATG CCATGGAGAG ACAGCTGCAG
    AGTGTTGCAG AATTGGAACA GAAATGGCAG AGTGAAGTTG ATGATGCCAT GCAGGGGAAA
    CTGGAGAACA ACATGCCTTT CTTCTATGAT TACCATTTCA ATGAGAACAA AATGAAAGAA
    CTAGAACTTT TGTGTTCAAT GAAGGAAGTC TCCTTTGATG GAAATGATCT TGAAAACATG
    GTGCTATCTC TGAGGGAGAA GTTCCTACAA GAAGTGAATT CTCTTATTCA GAAACCCTCG
    CACCCACTGG CTAAAACGAA GACGTTAGTG AAGAGTCTAA TGAACCGAGC TGAGCTGTTG
    CTGCACGTCA CCATTGCAGC CCAGTCTGGC CTCACGAGAA GCATCTCAGG GACCCCCGCG
    GAAACACCGG CTTGTAAATC AGCCTCTGAA ACAAAGGTGA TCTCTCATGC TGTGCGTCAG
    CCTGTTTTCC TTCGCAGCAT GTCGGCTCCC TCTGACCTGG AAATGATCGG TAATGAAGAT
    TTGGAATTTA CTAGAGCAAA TCAGAGGCGG CGCCACGTGA CCAGCCACCG CAGCAGCTCC
    TTCACACTCC TGCAGTCATT GGCCATAGAG GACAGCAGGG ACAAGCCCAC CTACAGCGTC
    CTGCTGGGGC AGCTGTTTGC TTTCATCGGC ACCAACCCTG ACCAAGCTGT GTCCAGCAGC
    AGCTTTCTTC TGGCCGCACA GACGAGATGG CGGCGGGGAA ACACCCGCAA GCAGGCGTTG
    GTGCACATGC GGGAGCTGCT GACGGCTGCC GTGCGAGTCG GGGGAGTGAC ACACCTTGTG
    GGTCCAGTGA CAATGGTCCT GCAGGGAGGA CCCAGAATTG AAGAACTGAC CTGTGGTGGG
    ATGGTCGAGC AGGTCCAGGA AGCCTTCGGT GAGACCATGA CCTCTGTGGT GTCTTTGTGC
    GCACGTTACC CTATCGCATG TGCAAATAGC ATCGGACTCT TGTGCACTAT ACCTTATACC
    AGGAGTGAAG AGAAGTGCCT GGTGCGGAGT GGCCTTGTCC AGCTCATGGA TCGACTATGC
    AGCTTGAGCA GTCAGACTGA GTCCAGCTCC AGTGAGAAAC AAACCAAGAA GCAGAAGGTG
    GCCACCATGG CCTGGGCAGC CTTCCAGGTG CTTGCCAACC GCTGCGTTGA ATGGGAAAAA
    GAAGAAGGCG GCTCCACAGA GGCTGTGCAC TCAGGTCTGG CCCGTCAGGT GTCCAGCCTT
    CTCACGAATC ACCTTGCCCG AGCGACCGAG TGCTGTGGCA ACCAGGCTGC CGGGAACGAT
    GCCCTCCAGG ACGTCCTGAG TCTCCTCAAT GATCTCTCGA GGAGTCATAT AGGTAAAGCC
    ATCCTGAGCC AGCCAGCTTG TGTGTCCAAA CTCCTCTCCC TGCTACTTGA CCAACGCCCA
    TCCCCAAAGC TGGTTCTTAT CATTCTTCAG CTGTGCCGGG CGGCACTCCC GCTGATGAGC
    GTAGAAGACT GTGGGAACGT GGAGCTCCCA CCCTGGAGCT ACTCCGTCCC CTCCCTAAAC
    AGCGAGCAGG AGGATCCCAG CGACCCAGCG TCCAAGATCG CCTCGCTGCT CTTGGCAAAG
    CTGGCAGACT ACGTGGTCCC AGGATGTCAG ACAGTCCTTT CTCCAACCGC TTCCGAACCT
    GACACCACAT TGACAAAAAC CAGCCCCAAG AATTCCTTAA AAGGCGATAA AGATCCTGGA
    GAAGAGAGTG AAGCAGTGGA TGGCAAGCTC TCTATCTTCA TCCACAAGCG GGAAGACCAG
    TCGTCCCATG AAGTTCTGCA GCCATTACTA AGTAGCTCAG AAGGACGGCC CTTCCGACTT
    GGTACTGGTG CCAACATGGA GAAAGTTGTG AAAATGGACC GAGACATGAC GAAGGGTGGC
    TGCTGTGAGG TGATTACGGA AGAGGCGGCA GCTGCTCTGC GAAAAGCGAC CAAGTGGGCA
    CAGTCAGGCC TCATTGTCAG TGTTGGGCCA CCCGTGGAGT CCCTCAACCC AGAGGCCGTG
    AGCGGGCTGT CCACAGGGGA CAAGAAGAAG ACTGCCCAAA CCTCCATTTG CCGAGAGAGA
    AACTCTGAAC TCGCCAGGAC CGACCCCGTC CGACCTTTCA TCAGTGGGCA CGTGGCAAAC
    AGCATGGCTG CTGAGGTGAT CGCCTTGCTT CATAGCTTGC TGATGGCACC TGAGTCAAAT
    GCTGCTCAGA TATGGACCAC GACAGCAGAG AAGGTTCTGT CTCGTGCACT GATGTACATT
    CCACAGTTGG GGAAATATGC AGAAAGCATT CTGGAAAATG GCAGCAGCAG TGGCAGAAAG
    CTTGCCAAAC TTCAGAGAAT TGCCCGCCAG GCTGTGGCTG CACTCTGTGC ACTTGGAGGC
    TTCAAAGAGA CAATCAAGAT CGGGTCTGAG GTCCAGGTTT TAGGTAGAGG AATATCAGGA
    AGCATCGGAG TGGTGGCTTC TATCAACGAA CAGGAAGGTA TAGCTACGGT CAGATTCCCA
    CCCATAGACT GTAGAAAGAC TTCGCAAGCA TCAGACACAC TGACTATTCC ATTGTCTAGA
    CTTTGTGTTC CAAGATCAGA GGCATTGCCT CTTCATAAAC TGTCCATTAC TGAGAAGGTA
    GTACAGGCAG TCCAGTCCAT GTTGCTTCCT CAGGAGGGAA GTCTCTCTAT TCATACCTCA
    CTTCCTGCAG CAGGAGATGG GTCAGCTCCT GTGATGGCAG TCGTTCGGCT CCTGGCTGAA
    ATAAGAACAA GAGCATGCCT GGTCATGGCC CAGCTTTTAG AAGACAGCTT ATTTTGTGAA
    GAATTCATAC AGCAGTGTCC AGCAGCTGTT GAAGTTCTTA ACCTAGTAGC CCAGGAATGC
    AGTGCTGGAG AGCGGCTGGC AGTTGTGGAA GTGCAGTGTG AACGGCTGAG GATGCTCTAC
    CGGGACTGTG CTCGGCCCCC GCCGCCTCCG CTGCAGGCTG ACCGGAGACA GCCCAAAGAG
    ATTACGTGGA GCCCCTCACG AGTGTTTCCA CCTGTCCGAG CCTGCATGTT CTCCTCGCAC
    CTCACTTCTG TCACCTTCCT AGCAGACCCC AGTGCAGGGG GCGGTCTGCC CAGGGGCACA
    TTTATCTACG CCACCTCACC ACTGCCCGTT CAGGCCCCGT CTTTCTACTG GGAAATTGAA
    ATTGTTTCCT ATGGAGATAC AGATGATGAT ACTGGACCAA TAGTTTCCTT TGGCTTTGCT
    ACAGAAGCAG AGAAAAGAGA TGGGGCATGG ACTAATCCAG TGGGCACCTG TCTTTTCCAT
    AACAATGGCC GAGCTGTGCA TTACAATGGC TCCAGTTTAT TACAGTGGAA GAGCGTTCGA
    TTAGACGTAA CTCTCTCTCC TGGTGACGTG GCAGGGATTG GCTGGGAGAG AACCGAGGGG
    ACGCCTCCTC CACCCGGGCA GCCTGCCAAG GGCCGTGTGT ACTTCACATA CTGTGGGCAG
    CGCCTCTCAC CGTATCTCGA AGATGTCTCG GGTGGGATGT GGCCGGTGGT TCATATTCAG
    AAAAAGAACA CCAAGACTCG CGCTAATTTT GGCTCCCGCC CATTTGCCTA CGCTGAAGGG
    CAAGCCCACC GCAATGCTGC CGACCTGTGC ACTGACCTGG CCGAGGAAAT TAGCGCTAAC
    TTCGAGGCCC TGCCCTTTGC CATGGCCTCT GACAGTGATA ACGATGCTGG CACCAGTATT
    GCGTCAGACC CGGGCACTCA TGGGCCACCA TGCCGAATTG CTGCCGTAGC CACTGCTCAG
    CAACAATATG ATAGTGATAC TTCCTGTCAT TATAAAGTGG AGCTCAGCTA TGAGAATTTC
    ATCACCTCTG GGCCAGACCC TCACCCACCC CCCATTGCAG ATGACGAAAG TGATGATGAT
    GATGACGATG ACATCCCACA GGAGGATCAC TATGCCCTGC TGGTGAAAGC CTGGGAGACC
    AAGGTTTTTC CTACCATCCG AAGACGGTTC CGCAACGAAG CAGAGAGGAA ATCAGGACTG
    GACCAGATAA AGGGTGCTTT ACAACTAGGC ATGGTGGATA TTGCCCGGCA GACAGTTGAA
    TTCCTCTACG AGGAGAATGG TGGCATCCCA AGAGACCTTT ATCTTCCCAC CATTGAAGAC
    ATTAAAGATG AAGCGAACAA GTTCACAATT GATAAAGTTC GAAAAGGTCT CACGGTAGTA
    ACACGCTCTC CAGACAGCAA TAACGTAGCC AGCAGCACTG TTGGAACTGC CCTGCCAAAA
    TTTGCTATCC GAGGGATGCT GAAAACCTTT GGGCTTCATG GAGTTGTCTT AGATGTTGAT
    TCAGTTAATG AACTAGTTCA GGTAGAAACG TACCTCCGAA GTGAAGGTGT GCTGGTCCGA
    TACTGGTACC CTATTGATAT GTTGGAAAGG CCCCCCGCAG GCTACAGAAG GACTGCCACC
    AATGGGCTCG TCACACTGGA CAATACCAAC CTTCAAATTC ACAGGGAGCT GCTGCGCTGC
    GAGGCCGCGC TGGCCAGGCT GTACTGCCGC ATGGCCCTGC TCAACATCTT CGCCCCCAAG
    CTGCCTCACC TGTTTACCCG CCTCTTCCAC ATCCCCGCCA TCCGGGACAT TACCCTGGAG
    CACCTGCAGC TGCTGTCCAA TCAGCTCCTC GCTCCTCCCC TCCCAGATGG CACCATCAGC
    TCCAGCTCCA TCCTCCTGGC GCAGTCCCTG CAGCACTGTG TCCATTCCCA GAACTGTTCG
    GCCACAGACC TCTTCTACCA GGGCAGCTCC CAGACGGTGC GAGAGTGGCT CAACGTGGCC
    ATCACGCGGA CCCTGCACCA GGGCGAGGAA AGCCTTCTGG AGCTGACCAA ACAGATCTGC
    TCTTTCCTGC AGACAGCACC AGAACAGTTC CCCTCCGAAG AGTTTCCAAT TTCCGAGTCC
    AAAGTCAACA TGGACGTCAA TTTCCCCGGG GCAGCTTTCG TCGTCGTATC TTGCAAAGAA
    AGTCAGTCTG GATTCCGCAA AGATTCCTCT CTGTACAAGG CACCATGGGC GCGTGTGCTG
    GTGTACGGGC TAGGCCACAA AGTGAAGCGA AATGGCCAGC TGAACCTCAT TGAGGCCGTC
    TGTTACCCGC GGGATGCGTC TCCGGCCAAC ACAGGGCTCA CACCACCTCC TACCACCAAC
    CAGTACCCTT CTGTGATCCT CTCCACAGAC AAAGTCCACA TCAAACTGGG GGTATCTCCC
    CCACCCGGAG CCGTGCTGGT GTTACACTCC CTGCCTCTCG AGTTCCCCCT GGCGATGGCC
    TTCACCGAGC AGCTACTGTC CTGGAAATCA GAGGATGGCG AAGGTAGAGC TGAAGATGAG
    CCTGACACCA TCCCGACGTC CGTCCTCCTG CAAGTGGTGG AGCTGCTTGG CAACTTCCTC
    TGGACCACAG ACATGGCGGC CTGCGTGAAG GAGCTGGTTT TCCACCTCCT GGCAGAGCTC
    CTGCGCACGG TGCACAGCCT AGAGCAGAGG AAGCACCCAG CCGGCCTGTC CTCATCCATC
    GCCCTCCAGC TGAACCCCTG CCTGGCCATG CTGATGGCCC TGCAGTCGGA GCTTCACAAG
    CTGTACGACG AGGAGACGCA GAGCTGGGTC TCGGGCAGTG CATGCGGAGG CTCAGGAGCC
    ACGGCGGCTG CTGACCAGGG CCGGTTCTCG ACGTATTTCC ACGCACTCAT GGAGGGCTGC
    CTGGCGGTGG CCGAGGTGAC CCTGCCCACC AACATGAGTG TCACCGCCAG CGGGGTGACG
    TCAGCAACTG CCCCAAACCT CAGCGACTCG TCCTCCTCCT CATCGTCCTC CCCAGGACAG
    ACTCCACAGA GCCCCAGCCT CCTGTCCAAG AGGAAGAAAG TCAAGATGAA GCGGGAAAAG
    GCCTCCTCCT CTGGGAAGCG CCAGTCCTCA CGCTCTGTGG ACTCGGACCC CGCCGTGCTG
    AGCATCGGGG GCAGCAAGCC CGAGGACATG CTGTGGTTCC ATCGTGCTCT GACGTTGCTC
    ATCATCCTCC GCCACCTCAC CAGGAAGGAC CCACAGGGGC TAGGCGTGAC AAGCGACGCC
    ATCGCCGACG CCTGCCAGGC CCTGGTGGGC CCCACCGCCC ACAGCCGCTT GCTGGTGATC
    TCTGGGATCC CCACCCACCT GGACGAGGGC ATCGTCAGGG GTGCCATCCG CAAGGCCTGC
    AATGCCCATG GCGGGGTGTT CAAGGATGAG ATCTACATCC CCCTGCAGGA CGAAGACCCC
    AAGAAGCCAA AAGATAAGGC CGAGGGGGGT GATGGGAAAG CCGAGCCGGA AAAGACCCTC
    GGCTTCCCCA GCACGGACAG CTTGGAGGTC AGCACATCCA GCAGCCTGAC CCCCGCTATG
    AGCGTCAGCG CATCCGCGTC CACCAGCCAG GCTTCCATCT GCAGCTCCCA GGGCATCTCG
    CAGACAGTCA GTGACCTCTC TGCGGATCCG TTGCCTGCAG GCCTGGAGCT GCCCATCCCT
    GCAGGCTTGT TGGAGCCCCA TGTGGTGTCC AGCCAGGAGA GCCTGGACAT TTCCCTGTGT
    AGCACCGGCA GCCTGGGCAG CCTGGGGAGC CTGGGCGAGC CACTGGACAA TGCAGAAACG
    GCCTCAGTGT CAGACATGGG CTCCATGTAC ACAGTCACGT CCCTGGACAA CCAGCCCCTT
    GCAGCGCGCC CCATCAAAGG CTTCGCGGTC GTGGAGATAA GGTCCCGAGC CAAAATTGAG
    AAAATTCGAG CAAGTTTATT TAACAACAAT GACTTGATAG GTTTGTCAAG CTTGGATGGT
    GAGGATGAAT TGATGGAAAT GTCAACGGAA GAGATCCTCA CGGTGTCTGT AGTGAATCAG
    AGTCTATTCG ATACGCAGGG GAGCCCAGGG TTAGAAGATT ATTTCAATGA TAAGTCAATT
    AAAGGTGAGA AGCTGGTGCC TGGGGCCAGA GAGGTGCTGA CGGAGATATT TAAGAGCTGT
    GCCCACTCGG AGCAGATGCT GAGCCTGACG CCAGCAAAGC CCATCAAAGT GTCGGACATT
    TATCTTAGCA AAGAGCAGAT CAACTCCCAG ACACCGGGCA ACCTCCTCCA CCTTTTCTTC
    ACCAATGTCC GGCCCCCCAA AAAGGTGCTA GAGGACCAGC TCACCCAGAT CCTGAGGAAG
    TACGGTGTGC CGAAGCCCAA GTTTGACAAG AGCAAGTACA GCAAGGCGGG GAAGGAGCAG
    CACCCCGGGA AGGTGGTGAG CACCAAGCGG CCCATCACCA AGCCGCCCAC CAAGGACAAG
    GCAGTGCTCA ACAGCGTCAG CAGGACTGCC TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC
    AAGTCACCTG AAAAGAGCAA GCCAGATGAA AAGGACCCTG AAAAGTCACC CACCAAGAAA
    CAAGACGTCC CAGAGGAGAA GTACCTGACC TTAGAAGGAT TTCACAAGTT TGTGATCGAC
    CGAGCCAAGC AAGACATCCG CAGCGTCTGG AGAGCGATTT TATCCTGTGG CTACGATCTT
    CATTTTGAGA GGTGTGCCTG CATCGACGTC CGGCATGCGC ACAAGGCCTC CAGGAAGTGG
    ACCCTGGAGA TGGATGTGGC GCTCGTGCAG TACATCAACC GGCTGTGCCG CCACCTGGCC
    ATCACGCCCG CGCGTCTGCA TCCCCACGAG GTGTATCTGG ACCCTGCAGA CGCTGCTGAC
    CCCAGAGTGG CCTGTCTCTT GAACGTGCCC ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG
    CTGCAGTCCC TCAACACCAC GCTCGAGACC TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG
    ACTCCCATGT ACACGCACAG CATCGCCGCC CTGCTCAAGG AGGCCAAAGG GCTGATCTTT
    TATGACACAA AGGTGACCGT CATGAATCGG GTGCTAAATG CCACGGTGCA GAGGACGGCT
    GACCATGCAG CACCAGAAAT CACTCTGGAC CCGCTGGAGA TCGTGGGAGG GGAGATCAGA
    GCCTCTGAGA ACTCCTACTT CTGCCAGGCA GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC
    CAGCTTTGCG TCAAACTGGC GAGTGGCGGC GACCCTACCT ATGCCTTCAA CATCCGCTTC
    ACGGGAGAGG AGGTCCATGG CACCAGTGGC TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC
    AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC
    AAGAACAAGG GCAAGTACAT CCTGACCCCC AGCCCCATCA CCTATGGGGA GGAGCAGCTG
    CTGCACTTCC TGGGCCAGTT GCTAGGGATC GCCATCCGAG CAGATGTCCC CCTCCCCCTG
    GACCTCCTGC CCTCCTTCTG GAAGACACTG GTGGGCGAGC CCCTGGACCC TGACCAAGAC
    CTGCAGGAAG CAGACGTACT CACCTACAAT TACGTCAAGA AATTTGAGAG TATCAACGAC
    GAGACGGAGT TGGAGGCCCT GTGTGCTGAG ATCGCCTCCC AGCACCTGGC CACTGAGAGC
    CCCGAGGGGC CCAACAAGCC CTGCTGCCGG TTCACCTACC TGACCATGAC CGGCGAGGAG
    GTGGAGCTGT GCAGCCGTGG CCGGCACATC CCCGTGGCGT GGGAGAACAA GGACATCTAT
    GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT GAGCTACAGA ACGTGGAGTG TGTGACGGCC
    GTGCGCGCGG GCCTTGGGGC CATCATCCCC CTGCAGCTGC TCACCATGCT CAGCCCACTG
    GAGATGGAGC TGCGCACCTG CGGCCTCCCG TACATCAACC TCGAGTTCCT GAAGGCCCAC
    ACCATGTACC AGGTGGGGCT GATGGAGACC GACCAGCACA TCGAGTTCTT CTGGGGGGCA
    CTGGAGATGT TTGCCCAGGA GGAGCTGTGC AAGTTCATCA AGTTTGCCTG CAACCAGGAG
    CGCATCCCGT TCACCTGCCC CTGCAAAGAC GGGGGTCCTG ACACGGCCCA TGTGCCCCCA
    TACCCCATGA AGATTGCCCC CCCAGATGGC ACAGCAGGTT CCCCAGATTC TCGCTACATC
    CGAGTGGAGA CCTGTATGTT CATGATCAAG CTTCCCCAGT ACTCCTCGCT GGAAATCATG
    CTGGAGAAAC TACGTTGTGC CATTCACTAC CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93228
    Clone ID Related Accession (Same CDS sequence) XM_015467162.1 , XM_015475527.1
    Accession Version XM_015467162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
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    1321
    1381
    1441
    1501
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    1621
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    1981
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    11101
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    11221
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    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322648.1
    CDS1027..12750
    Translation

    Target ORF information:

    RefSeq Version XM_015467162.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93228
    Clone ID Related Accession (Same CDS sequence) XM_015467162.1 , XM_015475527.1
    Accession Version XM_015475527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11724bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    11401
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    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331013.1
    CDS1027..12750
    Translation

    Target ORF information:

    RefSeq Version XM_015475527.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    ATGCTTCGGA AGACACCCAT TTATACCTGT GGTACATACT TGGTGATGCT GGTCCCACCT 
    CCAGGGGGAT CAGGCAGCTC TGCCACCCGC TCTCTTTTTG GTGGAACAAG TGGTTTGTCG
    TCTTTAAAAA TCCTGGCCTC TAGCTTGGTG TATAATATTT CGGATGGTCA ATTCACAAGT
    CGTGCCGACC TCATAGATGC TGCTGGAAGC TCCTTGGGGC GTGGTGCTCT TGTACCAGGA
    TTGGGTGCCT GTTATGATAC AGTGAACAAC ATGCTGTGGA CATGCTCAAA TGACTATATC
    GATCAGTGGT GTAATCCTGG GAATCAGGCC TTCCATTACG TCTGCCAGAG ACTTGGAGTC
    AGCCATATCA TCACTGAGCC CAAAGAAGAG GCTATAACCA CGAATGAGGT TATAAACCAA
    TTATTGCACC ACGTTGGTGC GATGTGCATA CACCAACTCA ATCTTCTTGC TACCAACCCC
    AATCTTCCAA TCACAAGTGT CTTGGGCAAA CAGCATCCCA TTGAAGCACA CCATCTGAGC
    AGTATTTGTG ACATTATGGA GAAGGCCATG GTTAATGGAG ACACCTGTAT TATACGCTGC
    ATTCTTGTTG TCTTTCAGGT GGTGTTTAAA TTTTTCTTCA GCCCACAAAC TGAAAGGAAT
    CGAGACATCA TTCGACGGTC AGGATTGCTT CTTTGGCAGT TGTTGATGGC ACCCAAAGAT
    CAAATTTGCC CTGAAATTCA GAAGGAGGTC TGCCTTGCCA TCAGCTCTGG TTTAAATATC
    TTGTACCCAG GTGAAACTGA AATCAATAAC TTACTTAAGC TGGTCTTAAC AGAAGGAGAG
    AGAAACAGTG GCCTCTCCCA GCTGCGGGAT GTGATCCTCA CTAATTTAGC TGAACAACTT
    CAAAACAACC GATTCGGCAG CGAGGAGGAG GATCATTACA GACTAAATGA TGAACTTTTA
    CACTACATTC TGAAGATTGT TGTACGAGAA TCCTGTATCT TAATCACCAA GTGCCAAACT
    GTCTCTAAAG ATGATTTTCA AAAACTCCTT TCAACTGTGC CTGCCGCATC CTCCTGCCTG
    CGCTATCTGA TGGCAGTTCA GAACCACCTT CTCAGTAACA CTATTTTGAT TAAGCCTGAT
    GAGAATGATG ACAGTGACAA CTCCTTGCAG GGAGAGACAT TGAAGGTACA GGAGTTAAAG
    GTCAGTATTT TGGCTCTTGC TACCCAAATC CTGACTGGAT GTGATGAAGT GTTGGAAATG
    CTACAGCAGG TCACAACTGC CCTCATAAAT AGTGACATAC CCGACCGGGA GCAGAGGTTA
    AAAGGCTTGG AACAAGTTAC TAAGGCTACT ATGCTTGGTC ACCTTCTCCC AGTGTTACTC
    ACTTCCCTGA TGCATCCAAA TTTACAAACT TTGACCATGG CCGATGCCCT GATGCCTCAG
    CTAGTGCAGC TGGTACTCTA TACCAGTCAG ACGGCTTTGC TGCTTAAAAC CCAGTGTCCT
    GTTTTTGCTG AGGTGGGCTG TTCCCCATGT GGTGCATCAG ACCAGAAGGG CAGGCTTTTG
    CCGGATGAGA GAATGCTGGA AGAGAAGGAG GAGCCAGGGT TTCTCACTGG TTTAAAGATT
    CCTGCTCCAT GGGCTGCTGG GAAGACTGTG GAAACAGTCC ACCCTGTCAG AGACAACTAT
    AAATTTAAAG AAACTGTCCA CATCCCCGGA GCTCGCTGCC TGTACCTTAG ATTTGATAGC
    AGATGCTCTT CGCAATATGA CTACGACAAA TCTAACAGTA TATTTTCTGT TTTGAAGTTG
    GTGATATACG CGGGGCCTAA CACAAACAGT AGGAAGGTTG CTGAGTATGG AGGCAATACA
    CTGGGATATG GCAGCCGTAG TGTCTTAGGA ACTGGTTGGC CGAAAGACTT GGTGAAGGTG
    GAAGGAGATA CAGTCACCTT CTCCTTTGAA ATGAGAAGTG GCCGTGAACA CAACACTCCC
    GATAAGGCTA TGTGGGGCTT TGCTTGCACA GTTCGCGCTC AGGAGTCTTC GGAGGATGTG
    TCAGGAGGCT TACCCTTTCT GGTAGACCTG GCTTTAGGTC TATCTGTGTT AGCTTGTTCC
    ATGTTAAGAA TCCTATACAA CGGACCAGAA ATTACCAAAG AAGAAGAAAC CTGTCAGGAG
    CTGTTGCGGT CCAAACTTTT ACAAAGGTGC CAGTGGCAGG TAGAGGCCAA CGGTGTGATC
    TCCCCTGCCC TTACTCCAAG CCCATCTCCA CTGCCTCTGA CCATCGAGGA AGACAGAGAG
    TTCACTTACC CGTCTGATGT CCTCGTGCCT CCTGTTGGAA ACTACTTTGA CCTGCCCCGG
    ATCAGGCTGC CTCCAGGAAT CATGATAAAG CTCAGGGAAA TTTCTGGGCG TGCTAGACCA
    CAGTTTAGAC CAAGTATAAA AGAAGTAATT CAGCCAGATG TGATGGAGGA AATGGTGGTA
    TCATGTGTTA TTAAGCACTT GAACTTAGTT GATGCACTGC AGTCCCTAAT AAATTTCCAG
    TATCAAGAAG AACATGCTGA GGAATATGAT TTACTGTGTA AAATCATGGG AGAGACATTT
    AAGAAACTCA ATGCCATGGA GAGACAGCTG CAGAGTGTTG CAGAATTGGA ACAGAAATGG
    CAGAGTGAAG TTGATGATGC CATGCAGGGG AAACTGGAGA ACAACATGCC TTTCTTCTAT
    GATTACCATT TCAATGAGAA CAAAATGAAA GAACTAGAAC TTTTGTGTTC AATGAAGGAA
    GTCTCCTTTG ATGGAAATGA TCTTGAAAAC ATGGTGCTAT CTCTGAGGGA GAAGTTCCTA
    CAAGAAGTGA ATTCTCTTAT TCAGAAACCC TCGCACCCAC TGGCTAAAAC GAAGACGTTA
    GTGAAGAGTC TAATGAACCG AGCTGAGCTG TTGCTGCACG TCACCATTGC AGCCCAGTCT
    GGCCTCACGA GAAGCATCTC AGGGACCCCC GCGGAAACAC CGGCTTGTAA ATCAGCCTCT
    GAAACAAAGG TGATCTCTCA TGCTGTGCGT CAGCCTGTTT TCCTTCGCAG CATGTCGGCT
    CCCTCTGACC TGGAAATGAT CGGTAATGAA GATTTGGAAT TTACTAGAGC AAATCAGAGG
    CGGCGCCACG TGACCAGCCA CCGCAGCAGC TCCTTCACAC TCCTGCAGTC ATTGGCCATA
    GAGGACAGCA GGGACAAGCC CACCTACAGC GTCCTGCTGG GGCAGCTGTT TGCTTTCATC
    GGCACCAACC CTGACCAAGC TGTGTCCAGC AGCAGCTTTC TTCTGGCCGC ACAGACGAGA
    TGGCGGCGGG GAAACACCCG CAAGCAGGCG TTGGTGCACA TGCGGGAGCT GCTGACGGCT
    GCCGTGCGAG TCGGGGGAGT GACACACCTT GTGGGTCCAG TGACAATGGT CCTGCAGGGA
    GGACCCAGAA TTGAAGAACT GACCTGTGGT GGGATGGTCG AGCAGGTCCA GGAAGCCTTC
    GGTGAGACCA TGACCTCTGT GGTGTCTTTG TGCGCACGTT ACCCTATCGC ATGTGCAAAT
    AGCATCGGAC TCTTGTGCAC TATACCTTAT ACCAGGAGTG AAGAGAAGTG CCTGGTGCGG
    AGTGGCCTTG TCCAGCTCAT GGATCGACTA TGCAGCTTGA GCAGTCAGAC TGAGTCCAGC
    TCCAGTGAGA AACAAACCAA GAAGCAGAAG GTGGCCACCA TGGCCTGGGC AGCCTTCCAG
    GTGCTTGCCA ACCGCTGCGT TGAATGGGAA AAAGAAGAAG GCGGCTCCAC AGAGGCTGTG
    CACTCAGGTC TGGCCCGTCA GGTGTCCAGC CTTCTCACGA ATCACCTTGC CCGAGCGACC
    GAGTGCTGTG GCAACCAGGC TGCCGGGAAC GATGCCCTCC AGGACGTCCT GAGTCTCCTC
    AATGATCTCT CGAGGAGTCA TATAGGTAAA GCCATCCTGA GCCAGCCAGC TTGTGTGTCC
    AAACTCCTCT CCCTGCTACT TGACCAACGC CCATCCCCAA AGCTGGTTCT TATCATTCTT
    CAGCTGTGCC GGGCGGCACT CCCGCTGATG AGCGTAGAAG ACTGTGGGAA CGTGGAGCTC
    CCACCCTGGA GCTACTCCGT CCCCTCCCTA AACAGCGAGC AGGAGGATCC CAGCGACCCA
    GCGTCCAAGA TCGCCTCGCT GCTCTTGGCA AAGCTGGCAG ACTACGTGGT CCCAGGATGT
    CAGACAGTCC TTTCTCCAAC CGCTTCCGAA CCTGACACCA CATTGACAAA AACCAGCCCC
    AAGAATTCCT TAAAAGGCGA TAAAGATCCT GGAGAAGAGA GTGAAGCAGT GGATGGCAAG
    CTCTCTATCT TCATCCACAA GCGGGAAGAC CAGTCGTCCC ATGAAGTTCT GCAGCCATTA
    CTAAGTAGCT CAGAAGGACG GCCCTTCCGA CTTGGTACTG GTGCCAACAT GGAGAAAGTT
    GTGAAAATGG ACCGAGACAT GACGAAGGGT GGCTGCTGTG AGGTGATTAC GGAAGAGGCG
    GCAGCTGCTC TGCGAAAAGC GACCAAGTGG GCACAGTCAG GCCTCATTGT CAGTGTTGGG
    CCACCCGTGG AGTCCCTCAA CCCAGAGGCC GTGAGCGGGC TGTCCACAGG GGACAAGAAG
    AAGACTGCCC AAACCTCCAT TTGCCGAGAG AGAAACTCTG AACTCGCCAG GACCGACCCC
    GTCCGACCTT TCATCAGTGG GCACGTGGCA AACAGCATGG CTGCTGAGGT GATCGCCTTG
    CTTCATAGCT TGCTGATGGC ACCTGAGTCA AATGCTGCTC AGATATGGAC CACGACAGCA
    GAGAAGGTTC TGTCTCGTGC ACTGATGTAC ATTCCACAGT TGGGGAAATA TGCAGAAAGC
    ATTCTGGAAA ATGGCAGCAG CAGTGGCAGA AAGCTTGCCA AACTTCAGAG AATTGCCCGC
    CAGGCTGTGG CTGCACTCTG TGCACTTGGA GGCTTCAAAG AGACAATCAA GATCGGGTCT
    GAGGTCCAGG TTTTAGGTAG AGGAATATCA GGAAGCATCG GAGTGGTGGC TTCTATCAAC
    GAACAGGAAG GTATAGCTAC GGTCAGATTC CCACCCATAG ACTGTAGAAA GACTTCGCAA
    GCATCAGACA CACTGACTAT TCCATTGTCT AGACTTTGTG TTCCAAGATC AGAGGCATTG
    CCTCTTCATA AACTGTCCAT TACTGAGAAG GTAGTACAGG CAGTCCAGTC CATGTTGCTT
    CCTCAGGAGG GAAGTCTCTC TATTCATACC TCACTTCCTG CAGCAGGAGA TGGGTCAGCT
    CCTGTGATGG CAGTCGTTCG GCTCCTGGCT GAAATAAGAA CAAGAGCATG CCTGGTCATG
    GCCCAGCTTT TAGAAGACAG CTTATTTTGT GAAGAATTCA TACAGCAGTG TCCAGCAGCT
    GTTGAAGTTC TTAACCTAGT AGCCCAGGAA TGCAGTGCTG GAGAGCGGCT GGCAGTTGTG
    GAAGTGCAGT GTGAACGGCT GAGGATGCTC TACCGGGACT GTGCTCGGCC CCCGCCGCCT
    CCGCTGCAGG CTGACCGGAG ACAGCCCAAA GAGATTACGT GGAGCCCCTC ACGAGTGTTT
    CCACCTGTCC GAGCCTGCAT GTTCTCCTCG CACCTCACTT CTGTCACCTT CCTAGCAGAC
    CCCAGTGCAG GGGGCGGTCT GCCCAGGGGC ACATTTATCT ACGCCACCTC ACCACTGCCC
    GTTCAGGCCC CGTCTTTCTA CTGGGAAATT GAAATTGTTT CCTATGGAGA TACAGATGAT
    GATACTGGAC CAATAGTTTC CTTTGGCTTT GCTACAGAAG CAGAGAAAAG AGATGGGGCA
    TGGACTAATC CAGTGGGCAC CTGTCTTTTC CATAACAATG GCCGAGCTGT GCATTACAAT
    GGCTCCAGTT TATTACAGTG GAAGAGCGTT CGATTAGACG TAACTCTCTC TCCTGGTGAC
    GTGGCAGGGA TTGGCTGGGA GAGAACCGAG GGGACGCCTC CTCCACCCGG GCAGCCTGCC
    AAGGGCCGTG TGTACTTCAC ATACTGTGGG CAGCGCCTCT CACCGTATCT CGAAGATGTC
    TCGGGTGGGA TGTGGCCGGT GGTTCATATT CAGAAAAAGA ACACCAAGAC TCGCGCTAAT
    TTTGGCTCCC GCCCATTTGC CTACGCTGAA GGGCAAGCCC ACCGCAATGC TGCCGACCTG
    TGCACTGACC TGGCCGAGGA AATTAGCGCT AACTTCGAGG CCCTGCCCTT TGCCATGGCC
    TCTGACAGTG ATAACGATGC TGGCACCAGT ATTGCGTCAG ACCCGGGCAC TCATGGGCCA
    CCATGCCGAA TTGCTGCCGT AGCCACTGCT CAGCAACAAT ATGATAGTGA TACTTCCTGT
    CATTATAAAG TGGAGCTCAG CTATGAGAAT TTCATCACCT CTGGGCCAGA CCCTCACCCA
    CCCCCCATTG CAGATGACGA AAGTGATGAT GATGATGACG ATGACATCCC ACAGGAGGAT
    CACTATGCCC TGCTGGTGAA AGCCTGGGAG ACCAAGGTTT TTCCTACCAT CCGAAGACGG
    TTCCGCAACG AAGCAGAGAG GAAATCAGGA CTGGACCAGA TAAAGGGTGC TTTACAACTA
    GGCATGGTGG ATATTGCCCG GCAGACAGTT GAATTCCTCT ACGAGGAGAA TGGTGGCATC
    CCAAGAGACC TTTATCTTCC CACCATTGAA GACATTAAAG ATGAAGCGAA CAAGTTCACA
    ATTGATAAAG TTCGAAAAGG TCTCACGGTA GTAACACGCT CTCCAGACAG CAATAACGTA
    GCCAGCAGCA CTGTTGGAAC TGCCCTGCCA AAATTTGCTA TCCGAGGGAT GCTGAAAACC
    TTTGGGCTTC ATGGAGTTGT CTTAGATGTT GATTCAGTTA ATGAACTAGT TCAGGTAGAA
    ACGTACCTCC GAAGTGAAGG TGTGCTGGTC CGATACTGGT ACCCTATTGA TATGTTGGAA
    AGGCCCCCCG CAGGCTACAG AAGGACTGCC ACCAATGGGC TCGTCACACT GGACAATACC
    AACCTTCAAA TTCACAGGGA GCTGCTGCGC TGCGAGGCCG CGCTGGCCAG GCTGTACTGC
    CGCATGGCCC TGCTCAACAT CTTCGCCCCC AAGCTGCCTC ACCTGTTTAC CCGCCTCTTC
    CACATCCCCG CCATCCGGGA CATTACCCTG GAGCACCTGC AGCTGCTGTC CAATCAGCTC
    CTCGCTCCTC CCCTCCCAGA TGGCACCATC AGCTCCAGCT CCATCCTCCT GGCGCAGTCC
    CTGCAGCACT GTGTCCATTC CCAGAACTGT TCGGCCACAG ACCTCTTCTA CCAGGGCAGC
    TCCCAGACGG TGCGAGAGTG GCTCAACGTG GCCATCACGC GGACCCTGCA CCAGGGCGAG
    GAAAGCCTTC TGGAGCTGAC CAAACAGATC TGCTCTTTCC TGCAGACAGC ACCAGAACAG
    TTCCCCTCCG AAGAGTTTCC AATTTCCGAG TCCAAAGTCA ACATGGACGT CAATTTCCCC
    GGGGCAGCTT TCGTCGTCGT ATCTTGCAAA GAAAGTCAGT CTGGATTCCG CAAAGATTCC
    TCTCTGTACA AGGCACCATG GGCGCGTGTG CTGGTGTACG GGCTAGGCCA CAAAGTGAAG
    CGAAATGGCC AGCTGAACCT CATTGAGGCC GTCTGTTACC CGCGGGATGC GTCTCCGGCC
    AACACAGGGC TCACACCACC TCCTACCACC AACCAGTACC CTTCTGTGAT CCTCTCCACA
    GACAAAGTCC ACATCAAACT GGGGGTATCT CCCCCACCCG GAGCCGTGCT GGTGTTACAC
    TCCCTGCCTC TCGAGTTCCC CCTGGCGATG GCCTTCACCG AGCAGCTACT GTCCTGGAAA
    TCAGAGGATG GCGAAGGTAG AGCTGAAGAT GAGCCTGACA CCATCCCGAC GTCCGTCCTC
    CTGCAAGTGG TGGAGCTGCT TGGCAACTTC CTCTGGACCA CAGACATGGC GGCCTGCGTG
    AAGGAGCTGG TTTTCCACCT CCTGGCAGAG CTCCTGCGCA CGGTGCACAG CCTAGAGCAG
    AGGAAGCACC CAGCCGGCCT GTCCTCATCC ATCGCCCTCC AGCTGAACCC CTGCCTGGCC
    ATGCTGATGG CCCTGCAGTC GGAGCTTCAC AAGCTGTACG ACGAGGAGAC GCAGAGCTGG
    GTCTCGGGCA GTGCATGCGG AGGCTCAGGA GCCACGGCGG CTGCTGACCA GGGCCGGTTC
    TCGACGTATT TCCACGCACT CATGGAGGGC TGCCTGGCGG TGGCCGAGGT GACCCTGCCC
    ACCAACATGA GTGTCACCGC CAGCGGGGTG ACGTCAGCAA CTGCCCCAAA CCTCAGCGAC
    TCGTCCTCCT CCTCATCGTC CTCCCCAGGA CAGACTCCAC AGAGCCCCAG CCTCCTGTCC
    AAGAGGAAGA AAGTCAAGAT GAAGCGGGAA AAGGCCTCCT CCTCTGGGAA GCGCCAGTCC
    TCACGCTCTG TGGACTCGGA CCCCGCCGTG CTGAGCATCG GGGGCAGCAA GCCCGAGGAC
    ATGCTGTGGT TCCATCGTGC TCTGACGTTG CTCATCATCC TCCGCCACCT CACCAGGAAG
    GACCCACAGG GGCTAGGCGT GACAAGCGAC GCCATCGCCG ACGCCTGCCA GGCCCTGGTG
    GGCCCCACCG CCCACAGCCG CTTGCTGGTG ATCTCTGGGA TCCCCACCCA CCTGGACGAG
    GGCATCGTCA GGGGTGCCAT CCGCAAGGCC TGCAATGCCC ATGGCGGGGT GTTCAAGGAT
    GAGATCTACA TCCCCCTGCA GGACGAAGAC CCCAAGAAGC CAAAAGATAA GGCCGAGGGG
    GGTGATGGGA AAGCCGAGCC GGAAAAGACC CTCGGCTTCC CCAGCACGGA CAGCTTGGAG
    GTCAGCACAT CCAGCAGCCT GACCCCCGCT ATGAGCGTCA GCGCATCCGC GTCCACCAGC
    CAGGCTTCCA TCTGCAGCTC CCAGGGCATC TCGCAGACAG TCAGTGACCT CTCTGCGGAT
    CCGTTGCCTG CAGGCCTGGA GCTGCCCATC CCTGCAGGCT TGTTGGAGCC CCATGTGGTG
    TCCAGCCAGG AGAGCCTGGA CATTTCCCTG TGTAGCACCG GCAGCCTGGG CAGCCTGGGG
    AGCCTGGGCG AGCCACTGGA CAATGCAGAA ACGGCCTCAG TGTCAGACAT GGGCTCCATG
    TACACAGTCA CGTCCCTGGA CAACCAGCCC CTTGCAGCGC GCCCCATCAA AGGCTTCGCG
    GTCGTGGAGA TAAGGTCCCG AGCCAAAATT GAGAAAATTC GAGCAAGTTT ATTTAACAAC
    AATGACTTGA TAGGTTTGTC AAGCTTGGAT GGTGAGGATG AATTGATGGA AATGTCAACG
    GAAGAGATCC TCACGGTGTC TGTAGTGAAT CAGAGTCTAT TCGATACGCA GGGGAGCCCA
    GGGTTAGAAG ATTATTTCAA TGATAAGTCA ATTAAAGGTG AGAAGCTGGT GCCTGGGGCC
    AGAGAGGTGC TGACGGAGAT ATTTAAGAGC TGTGCCCACT CGGAGCAGAT GCTGAGCCTG
    ACGCCAGCAA AGCCCATCAA AGTGTCGGAC ATTTATCTTA GCAAAGAGCA GATCAACTCC
    CAGACACCGG GCAACCTCCT CCACCTTTTC TTCACCAATG TCCGGCCCCC CAAAAAGGTG
    CTAGAGGACC AGCTCACCCA GATCCTGAGG AAGTACGGTG TGCCGAAGCC CAAGTTTGAC
    AAGAGCAAGT ACAGCAAGGC GGGGAAGGAG CAGCACCCCG GGAAGGTGGT GAGCACCAAG
    CGGCCCATCA CCAAGCCGCC CACCAAGGAC AAGGCAGTGC TCAACAGCGT CAGGACTGCC
    TTGAGTGAGA AGAAGCCCAC CGTGAAGCCC AAGTCACCTG AAAAGAGCAA GCCAGATGAA
    AAGGACCCTG AAAAGTCACC CACCAAGAAA CAAGACGTCC CAGAGGAGAA GTACCTGACC
    TTAGAAGGAT TTCACAAGTT TGTGATCGAC CGAGCCAAGC AAGACATCCG CAGCGTCTGG
    AGAGCGATTT TATCCTGTGG CTACGATCTT CATTTTGAGA GGTGTGCCTG CATCGACGTC
    CGGCATGCGC ACAAGGCCTC CAGGAAGTGG ACCCTGGAGA TGGATGTGGC GCTCGTGCAG
    TACATCAACC GGCTGTGCCG CCACCTGGCC ATCACGCCCG CGCGTCTGCA TCCCCACGAG
    GTGTATCTGG ACCCTGCAGA CGCTGCTGAC CCCAGAGTGG CCTGTCTCTT GAACGTGCCC
    ATCGAGAGCC TGCGCCTGCG CTTCGCTCTG CTGCAGTCCC TCAACACCAC GCTCGAGACC
    TTCTTCCTGC CGCTGGTTGA GCTGCGCCAG ACTCCCATGT ACACGCACAG CATCGCCGCC
    CTGCTCAAGG AGGCCAAAGG GCTGATCTTT TATGACACAA AGGTGACCGT CATGAATCGG
    GTGCTAAATG CCACGGTGCA GAGGACGGCT GACCATGCAG CACCAGAAAT CACTCTGGAC
    CCGCTGGAGA TCGTGGGAGG GGAGATCAGA GCCTCTGAGA ACTCCTACTT CTGCCAGGCA
    GCCAGGCAGC TGGCCTCAGT GCCCTCCTCC CAGCTTTGCG TCAAACTGGC GAGTGGCGGC
    GACCCTACCT ATGCCTTCAA CATCCGCTTC ACGGGAGAGG AGGTCCATGG CACCAGTGGC
    TCCTTCCGCC ACTTCCTGTG GCAGGTGTGC AGAGAGCTGC AGAGCTCCTC GCTCTCCCTC
    CTCCTGCTGT GCCCCAGCTC CGCCGTCAAC AAGAACAAGG GCAAGTACAT CCTGACCCCC
    AGCCCCATCA CCTATGGGGA GGAGCAGCTG CTGCACTTCC TGGGCCAGTT GCTAGGGATC
    GCCATCCGAG CAGATGTCCC CCTCCCCCTG GACCTCCTGC CCTCCTTCTG GAAGACACTG
    GTGGGCGAGC CCCTGGACCC TGACCAAGAC CTGCAGGAAG CAGACGTACT CACCTACAAT
    TACGTCAAGA AATTTGAGAG TATCAACGAC GAGACGGAGT TGGAGGCCCT GTGTGCTGAG
    ATCGCCTCCC AGCACCTGGC CACTGAGAGC CCCGAGGGGC CCAACAAGCC CTGCTGCCGG
    TTCACCTACC TGACCATGAC CGGCGAGGAG GTGGAGCTGT GCAGCCGTGG CCGGCACATC
    CCCGTGGCGT GGGAGAACAA GGACATCTAT GCGGCCGCCA TCCGGAGCCT GCGGCTGCGT
    GAGCTACAGA ACGTGGAGTG TGTGACGGCC GTGCGCGCGG GCCTTGGGGC CATCATCCCC
    CTGCAGCTGC TCACCATGCT CAGCCCACTG GAGATGGAGC TGCGCACCTG CGGCCTCCCG
    TACATCAACC TCGAGTTCCT GAAGGCCCAC ACCATGTACC AGGTGGGGCT GATGGAGACC
    GACCAGCACA TCGAGTTCTT CTGGGGGGCA CTGGAGATGT TTGCCCAGGA GGAGCTGTGC
    AAGTTCATCA AGTTTGCCTG CAACCAGGAG CGCATCCCGT TCACCTGCCC CTGCAAAGAC
    GGGGGTCCTG ACACGGCCCA TGTGCCCCCA TACCCCATGA AGATTGCCCC CCCAGATGGC
    ACAGCAGGTT CCCCAGATTC TCGCTACATC CGAGTGGAGA CCTGTATGTT CATGATCAAG
    CTTCCCCAGT ACTCCTCGCT GGAAATCATG CTGGAGAAAC TACGTTGTGC CATTCACTAC
    CGCGAAGACC CTCTGAGTGG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93229
    Clone ID Related Accession (Same CDS sequence) XM_015475528.1 , XM_015467166.1
    Accession Version XM_015475528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1801
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    1981
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    11101
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    11221
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    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAATGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015331014.1
    CDS171..11819
    Translation

    Target ORF information:

    RefSeq Version XM_015475528.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015475528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAATGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93229
    Clone ID Related Accession (Same CDS sequence) XM_015475528.1 , XM_015467166.1
    Accession Version XM_015467166.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 11649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646371.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAATGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322652.1
    CDS171..11819
    Translation

    Target ORF information:

    RefSeq Version XM_015467166.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT domain containing E3 ubiquitin protein ligase 4 (HECTD4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467166.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    ATGCCATGGT CTCCCAAGAA CAAGCAGGCC CTCAAGTTCA TCAAGAAAAA AGTCCTGGCC 
    TCTAGCTTGG TGTATAATAT TTCGGATGGT CAATTCACAA GTCGTGCCGA CCTCATAGAT
    GCTGCTGGAA GCTCCTTGGG GCGTGGTGCT CTTGTACCAG GATTGGGTGC CTGTTATGAT
    ACAGTGAACA ACATGCTGTG GACATGCTCA AATGACTATA TCGATCAGTG GTGTAATCCT
    GGGAATCAGG CCTTCCATTA CGTCTGCCAG AGACTTGGAG TCAGCCATAT CATCACTGAG
    CCCAAAGAAG AGGCTATAAC CACGAATGAG GTTATAAACC AATTATTGCA CCACGTTGGT
    GCGATGTGCA TACACCAACT CAATCTTCTT GCTACCAACC CCAATCTTCC AATCACAAGT
    GTCTTGGGCA AACAGCATCC CATTGAAGCA CACCATCTGA GCAGTATTTG TGACATTATG
    GAGAAGGCCA TGGTTAATGG AGACACCTGT ATTATACGCT GCATTCTTGT TGTCTTTCAG
    GTGGTGTTTA AATTTTTCTT CAGCCCACAA ACTGAAAGGA ATCGAGACAT CATTCGACGG
    TCAGGATTGC TTCTTTGGCA GTTGTTGATG GCACCCAAAG ATCAAATTTG CCCTGAAATT
    CAGAAGGAGG TCTGCCTTGC CATCAGCTCT GGTTTAAATA TCTTGTACCC AGGTGAAACT
    GAAATCAATA ACTTACTTAA GCTGGTCTTA ACAGAAGGAG AGAGAAACAG TGGCCTCTCC
    CAGCTGCGGG ATGTGATCCT CACTAATTTA GCTGAACAAC TTCAAAACAA CCGATTCGGC
    AGCGAGGAGG AGGATCATTA CAGACTAAAT GATGAACTTT TACACTACAT TCTGAAGATT
    GTTGTACGAG AATCCTGTAT CTTAATCACC AAGTGCCAAA CTGTCTCTAA AGATGATTTT
    CAAAAACTCC TTTCAACTGT GCCTGCCGCA TCCTCCTGCC TGCGCTATCT GATGGCAGTT
    CAGAACCACC TTCTCAGTAA CACTATTTTG ATTAAGCCTG ATGAGAATGA TGACAGTGAC
    AACTCCTTGC AGGGAGAGAC ATTGAAGGTA CAGGAGTTAA AGGTCAGTAT TTTGGCTCTT
    GCTACCCAAA TCCTGACTGG ATGTGATGAA GTGTTGGAAA TGCTACAGCA GGTCACAACT
    GCCCTCATAA ATAGTGACAT ACCCGACCGG GAGCAGAGGT TAAAAGGCTT GGAACAAGTT
    ACTAAGGCTA CTATGCTTGG TCACCTTCTC CCAGTGTTAC TCACTTCCCT GATGCATCCA
    AATTTACAAA CTTTGACCAT GGCCGATGCC CTGATGCCTC AGCTAGTGCA GCTGGTACTC
    TATACCAGTC AGACGGCTTT GCTGCTTAAA ACCCAGTGTC CTGTTTTTGC TGAGGTGGGC
    TGTTCCCCAT GTGGTGCATC AGACCAGAAG GGCAGGCTTT TGCCGGATGA GAGAATGCTG
    GAAGAGAAGG AGGAGCCAGG GTTTCTCACT GGTTTAAAGA TTCCTGCTCC ATGGGCTGCT
    GGGAAGACTG TGGAAACAGT CCACCCTGTC AGAGACAACT ATAAATTTAA AGAAACTGTC
    CACATCCCCG GAGCTCGCTG CCTGTACCTT AGATTTGATA GCAGATGCTC TTCGCAATAT
    GACTACGACA AATCTAACAG TATATTTTCT GTTTTGAAGT TGGTGATATA CGCGGGGCCT
    AACACAAACA GTAGGAAGGT TGCTGAGTAT GGAGGCAATA CACTGGGATA TGGCAGCCGT
    AGTGTCTTAG GAACTGGTTG GCCGAAAGAC TTGGTGAAGG TGGAAGGAGA TACAGTCACC
    TTCTCCTTTG AAATGAGAAG TGGCCGTGAA CACAACACTC CCGATAAGGC TATGTGGGGC
    TTTGCTTGCA CAGTTCGCGC TCAGGAGTCT TCGGAGGATG TGTCAGGAGG CTTACCCTTT
    CTGGTAGACC TGGCTTTAGG TCTATCTGTG TTAGCTTGTT CCATGTTAAG AATCCTATAC
    AACGGACCAG AAATTACCAA AGAAGAAGAA ACCTGTCAGG AGCTGTTGCG GTCCAAACTT
    TTACAAAGGT GCCAGTGGCA GGTAGAGGCC AACGGTGTGA TCTCCCCTGC CCTTACTCCA
    AGCCCATCTC CACTGCCTCT GACCATCGAG GAAGACAGAG AGTTCACTTA CCCGTCTGAT
    GTCCTCGTGC CTCCTGTTGG AAACTACTTT GACCTGCCCC GGATCAGGCT GCCTCCAGGA
    ATCATGATAA AGCTCAGGGA AATTTCTGGG CGTGCTAGAC CACAGTTTAG ACCAAGTATA
    AAAGAAGTAA TTCAGCCAGA TGTGATGGAG GAAATGGTGG TATCATGTGT TATTAAGCAC
    TTGAACTTAG TTGATGCACT GCAGTCCCTA ATAAATTTCC AGTATCAAGA AGAACATGCT
    GAGGAATATG ATTTACTGTG TAAAATCATG GGAGAGACAT TTAAGAAACT CAATGCCATG
    GAGAGACAGC TGCAGAGTGT TGCAGAATTG GAACAGAAAT GGCAGAGTGA AGTTGATGAT
    GCCATGCAGG GGAAACTGGA GAACAACATG CCTTTCTTCT ATGATTACCA TTTCAATGAG
    AACAAAATGA AAGAACTAGA ACTTTTGTGT TCAATGAAGG AAGTCTCCTT TGATGGAAAT
    GATCTTGAAA ACATGGTGCT ATCTCTGAGG GAGAAGTTCC TACAAGAAGT GAATTCTCTT
    ATTCAGAAAC CCTCGCACCC ACTGGCTAAA ACGAAGACGT TAGTGAAGAG TCTAATGAAC
    CGAGCTGAGC TGTTGCTGCA CGTCACCATT GCAGCCCAGT CTGGCCTCAC GAGAAGCATC
    TCAGGGACCC CCGCGGAAAC ACCGGCTTGT AAATCAGCCT CTGAAACAAA GGTGATCTCT
    CATGCTGTGC GTCAGCCTGT TTTCCTTCGC AGCATGTCGG CTCCCTCTGA CCTGGAAATG
    ATCGGTAATG AAGATTTGGA ATTTACTAGA GCAAATCAGA GGCGGCGCCA CGTGACCAGC
    CACCGCAGCA GCTCCTTCAC ACTCCTGCAG TCATTGGCCA TAGAGGACAG CAGGGACAAG
    CCCACCTACA GCGTCCTGCT GGGGCAGCTG TTTGCTTTCA TCGGCACCAA CCCTGACCAA
    GCTGTGTCCA GCAGCAGCTT TCTTCTGGCC GCACAGACGA GATGGCGGCG GGGAAACACC
    CGCAAGCAGG CGTTGGTGCA CATGCGGGAG CTGCTGACGG CTGCCGTGCG AGTCGGGGGA
    GTGACACACC TTGTGGGTCC AGTGACAATG GTCCTGCAGG GAGGACCCAG AATTGAAGAA
    CTGACCTGTG GTGGGATGGT CGAGCAGGTC CAGGAAGCCT TCGGTGAGAC CATGACCTCT
    GTGGTGTCTT TGTGCGCACG TTACCCTATC GCATGTGCAA ATAGCATCGG ACTCTTGTGC
    ACTATACCTT ATACCAGGAG TGAAGAGAAG TGCCTGGTGC GGAGTGGCCT TGTCCAGCTC
    ATGGATCGAC TATGCAGCTT GAGCAGTCAG ACTGAGTCCA GCTCCAGTGA GAAACAAACC
    AAGAAGCAGA AGGTGGCCAC CATGGCCTGG GCAGCCTTCC AGGTGCTTGC CAACCGCTGC
    GTTGAATGGG AAAAAGAAGA AGGCGGCTCC ACAGAGGCTG TGCACTCAGG TCTGGCCCGT
    CAGGTGTCCA GCCTTCTCAC GAATCACCTT GCCCGAGCGA CCGAGTGCTG TGGCAACCAG
    GCTGCCGGGA ACGATGCCCT CCAGGACGTC CTGAGTCTCC TCAATGATCT CTCGAGGAGT
    CATATAGGTA AAGCCATCCT GAGCCAGCCA GCTTGTGTGT CCAAACTCCT CTCCCTGCTA
    CTTGACCAAC GCCCATCCCC AAAGCTGGTT CTTATCATTC TTCAGCTGTG CCGGGCGGCA
    CTCCCGCTGA TGAGCGTAGA AGACTGTGGG AACGTGGAGC TCCCACCCTG GAGCTACTCC
    GTCCCCTCCC TAAACAGCGA GCAGGAGGAT CCCAGCGACC CAGCGTCCAA GATCGCCTCG
    CTGCTCTTGG CAAAGCTGGC AGACTACGTG GTCCCAGGAT GTCAGACAGT CCTTTCTCCA
    ACCGCTTCCG AACCTGACAC CACATTGACA AAAACCAGCC CCAAGAATTC CTTAAAAGGC
    GATAAAGATC CTGGAGAAGA GAGTGAAGCA GTGGATGGCA AGCTCTCTAT CTTCATCCAC
    AAGCGGGAAG ACCAGTCGTC CCATGAAGTT CTGCAGCCAT TACTAAGTAG CTCAGAAGGA
    CGGCCCTTCC GACTTGGTAC TGGTGCCAAC ATGGAGAAAG TTGTGAAAAT GGACCGAGAC
    ATGACGAAGG GTGGCTGCTG TGAGGTGATT ACGGAAGAGG CGGCAGCTGC TCTGCGAAAA
    GCGACCAAGT GGGCACAGTC AGGCCTCATT GTCAGTGTTG GGCCACCCGT GGAGTCCCTC
    AACCCAGAGG CCGTGAGCGG GCTGTCCACA GGGGACAAGA AGAAGACTGC CCAAACCTCC
    ATTTGCCGAG AGAGAAACTC TGAACTCGCC AGGACCGACC CCGTCCGACC TTTCATCAGT
    GGGCACGTGG CAAACAGCAT GGCTGCTGAG GTGATCGCCT TGCTTCATAG CTTGCTGATG
    GCACCTGAGT CAAATGCTGC TCAGATATGG ACCACGACAG CAGAGAAGGT TCTGTCTCGT
    GCACTGATGT ACATTCCACA GTTGGGGAAA TATGCAGAAA GCATTCTGGA AAATGGCAGC
    AGCAGTGGCA GAAAGCTTGC CAAACTTCAG AGAATTGCCC GCCAGGCTGT GGCTGCACTC
    TGTGCACTTG GAGGCTTCAA AGAGACAATC AAGATCGGGT CTGAGGTCCA GGTTTTAGGT
    AGAGGAATAT CAGGAAGCAT CGGAGTGGTG GCTTCTATCA ACGAACAGGA AGGTATAGCT
    ACGGTCAGAT TCCCACCCAT AGACTGTAGA AAGACTTCGC AAGCATCAGA CACACTGACT
    ATTCCATTGT CTAGACTTTG TGTTCCAAGA TCAGAGGCAT TGCCTCTTCA TAAACTGTCC
    ATTACTGAGA AGGTAGTACA GGCAGTCCAG TCCATGTTGC TTCCTCAGGA GGGAAGTCTC
    TCTATTCATA CCTCACTTCC TGCAGCAGGA GATGGGTCAG CTCCTGTGAT GGCAGTCGTT
    CGGCTCCTGG CTGAAATAAG AACAAGAGCA TGCCTGGTCA TGGCCCAGCT TTTAGAAGAC
    AGCTTATTTT GTGAAGAATT CATACAGCAG TGTCCAGCAG CTGTTGAAGT TCTTAACCTA
    GTAGCCCAGG AATGCAGTGC TGGAGAGCGG CTGGCAGTTG TGGAAGTGCA GTGTGAACGG
    CTGAGGATGC TCTACCGGGA CTGTGCTCGG CCCCCGCCGC CTCCGCTGCA GGCTGACCGG
    AGACAGCCCA AAGAGATTAC GTGGAGCCCC TCACGAGTGT TTCCACCTGT CCGAGCCTGC
    ATGTTCTCCT CGCACCTCAC TTCTGTCACC TTCCTAGCAG ACCCCAGTGC AGGGGGCGGT
    CTGCCCAGGG GCACATTTAT CTACGCCACC TCACCACTGC CCGTTCAGGC CCCGTCTTTC
    TACTGGGAAA TTGAAATTGT TTCCTATGGA GATACAGATG ATGATACTGG ACCAATAGTT
    TCCTTTGGCT TTGCTACAGA AGCAGAGAAA AGAGATGGGG CATGGACTAA TCCAGTGGGC
    ACCTGTCTTT TCCATAACAA TGGCCGAGCT GTGCATTACA ATGGCTCCAG TTTATTACAG
    TGGAAGAGCG TTCGATTAGA CGTAACTCTC TCTCCTGGTG ACGTGGCAGG GATTGGCTGG
    GAGAGAACCG AGGGGACGCC TCCTCCACCC GGGCAGCCTG CCAAGGGCCG TGTGTACTTC
    ACATACTGTG GGCAGCGCCT CTCACCGTAT CTCGAAGATG TCTCGGGTGG GATGTGGCCG
    GTGGTTCATA TTCAGAAAAA GAACACCAAG ACTCGCGCTA ATTTTGGCTC CCGCCCATTT
    GCCTACGCTG AAGGGCAAGC CCACCGCAAT GCTGCCGACC TGTGCACTGA CCTGGCCGAG
    GAAATTAGCG CTAACTTCGA GGCCCTGCCC TTTGCCATGG CCTCTGACAG TGATAACGAT
    GCTGGCACCA GTATTGCGTC AGACCCGGGC ACTCATGGGC CACCATGCCG AATTGCTGCC
    GTAGCCACTG CTCAGCAACA ATATGATAGT GATACTTCCT GTCATTATAA AGTGGAGCTC
    AGCTATGAGA ATTTCATCAC CTCTGGGCCA GACCCTCACC CACCCCCCAT TGCAGATGAC
    GAAAGTGATG ATGATGATGA CGATGACATC CCACAGGAGG ATCACTATGC CCTGCTGGTG
    AAAGCCTGGG AGACCAAGGT TTTTCCTACC ATCCGAAGAC GGTTCCGCAA CGAAGCAGAG
    AGGAAATCAG GACTGGACCA GATAAAGGGT GCTTTACAAC TAGGCATGGT GGATATTGCC
    CGGCAGACAG TTGAATTCCT CTACGAGGAG AATGGTGGCA TCCCAAGAGA CCTTTATCTT
    CCCACCATTG AAGACATTAA AGATGAAGCG AACAAGTTCA CAATTGATAA AGTTCGAAAA
    GGTCTCACGG TAGTAACACG CTCTCCAGAC AGCAATAACG TAGCCAGCAG CACTGTTGGA
    ACTGCCCTGC CAAAATTTGC TATCCGAGGG ATGCTGAAAA CCTTTGGGCT TCATGGAGTT
    GTCTTAGATG TTGATTCAGT TAATGAACTA GTTCAGGTAG AAACGTACCT CCGAAGTGAA
    GGTGTGCTGG TCCGATACTG GTACCCTATT GATATGTTGG AAAGGCCCCC CGCAGGCTAC
    AGAAGGACTG CCACCAATGG GCTCGTCACA CTGGACAATA CCAACCTTCA AATTCACAGG
    GAGCTGCTGC GCTGCGAGGC CGCGCTGGCC AGGCTGTACT GCCGCATGGC CCTGCTCAAC
    ATCTTCGCCC CCAAGCTGCC TCACCTGTTT ACCCGCCTCT TCCACATCCC CGCCATCCGG
    GACATTACCC TGGAGCACCT GCAGCTGCTG TCCAATCAGC TCCTCGCTCC TCCCCTCCCA
    GATGGCACCA TCAGCTCCAG CTCCATCCTC CTGGCGCAGT CCCTGCAGCA CTGTGTCCAT
    TCCCAGAACT GTTCGGCCAC AGACCTCTTC TACCAGGGCA GCTCCCAGAC GGTGCGAGAG
    TGGCTCAACG TGGCCATCAC GCGGACCCTG CACCAGGGCG AGGAAAGCCT TCTGGAGCTG
    ACCAAACAGA TCTGCTCTTT CCTGCAGACA GCACCAGAAC AGTTCCCCTC CGAAGAGTTT
    CCAATTTCCG AGTCCAAAGT CAACATGGAC GTCAATTTCC CCGGGGCAGC TTTCGTCGTC
    GTATCTTGCA AAGAAAGTCA GTCTGGATTC CGCAAAGATT CCTCTCTGTA CAAGGCACCA
    TGGGCGCGTG TGCTGGTGTA CGGGCTAGGC CACAAAGTGA AGCGAAATGG CCAGCTGAAC
    CTCATTGAGG CCGTCTGTTA CCCGCGGGAT GCGTCTCCGG CCAACACAGG GCTCACACCA
    CCTCCTACCA CCAACCAGTA CCCTTCTGTG ATCCTCTCCA CAGACAAAGT CCACATCAAA
    CTGGGGGTAT CTCCCCCACC CGGAGCCGTG CTGGTGTTAC ACTCCCTGCC TCTCGAGTTC
    CCCCTGGCGA TGGCCTTCAC CGAGCAGCTA CTGTCCTGGA AATCAGAGGA TGGCGAAGGT
    AGAGCTGAAG ATGAGCCTGA CACCATCCCG ACGTCCGTCC TCCTGCAAGT GGTGGAGCTG
    CTTGGCAACT TCCTCTGGAC CACAGACATG GCGGCCTGCG TGAAGGAGCT GGTTTTCCAC
    CTCCTGGCAG AGCTCCTGCG CACGGTGCAC AGCCTAGAGC AGAGGAAGCA CCCAGCCGGC
    CTGTCCTCAT CCATCGCCCT CCAGCTGAAC CCCTGCCTGG CCATGCTGAT GGCCCTGCAG
    TCGGAGCTTC ACAAGCTGTA CGACGAGGAG ACGCAGAGCT GGGTCTCGGG CAGTGCATGC
    GGAGGCTCAG GAGCCACGGC GGCTGCTGAC CAGGGCCGGT TCTCGACGTA TTTCCACGCA
    CTCATGGAGG GCTGCCTGGC GGTGGCCGAG GTGACCCTGC CCACCAACAT GAGTGTCACC
    GCCAGCGGGG TGACGTCAGC AACTGCCCCA AACCTCAGCG ACTCGTCCTC CTCCTCATCG
    TCCTCCCCAG GACAGACTCC ACAGAGCCCC AGCCTCCTGT CCAAGAGGAA GAAAGTCAAG
    ATGAAGCGGG AAAAGGCCTC CTCCTCTGGG AAGCGCCAGT CCTCACGCTC TGTGGACTCG
    GACCCCGCCG TGCTGAGCAT CGGGGGCAGC AAGCCCGAGG ACATGCTGTG GTTCCATCGT
    GCTCTGACGT TGCTCATCAT CCTCCGCCAC CTCACCAGGA AGGACCCACA GGGGCTAGGC
    GTGACAAGCG ACGCCATCGC CGACGCCTGC CAGGCCCTGG TGGGCCCCAC CGCCCACAGC
    CGCTTGCTGG TGATCTCTGG GATCCCCACC CACCTGGACG AGGGCATCGT CAGGGGTGCC
    ATCCGCAAGG CCTGCAATGC CCATGGCGGG GTGTTCAAGG ATGAGATCTA CATCCCCCTG
    CAGGACGAAG ACCCCAAGAA GCCAAAAGAT AAGGCCGAGG GGGGTGATGG GAAAGCCGAG
    CCGGAAAAGA CCCTCGGCTT CCCCAGCACG GACAGCTTGG AGGTCAGCAC ATCCAGCAGC
    CTGACCCCCG CTATGAGCGT CAGCGCATCC GCGTCCACCA GCCAGGCTTC CATCTGCAGC
    TCCCAGGGCA TCTCGCAGAC AGTCAGTGAC CTCTCTGCGG ATCCGTTGCC TGCAGGCCTG
    GAGCTGCCCA TCCCTGCAGG CTTGTTGGAG CCCCATGTGG TGTCCAGCCA GGAGAGCCTG
    GACATTTCCC TGTGTAGCAC CGGCAGCCTG GGCAGCCTGG GGAGCCTGGG CGAGCCACTG
    GACAATGCAG AAACGGCCTC AGTGTCAGAC ATGGGCTCCA TGTACACAGT CACGTCCCTG
    GACAACCAGC CCCTTGCAGC GCGCCCCATC AAAGGCTTCG CGGTCGTGGA GATAAGGTCC
    CGAGCCAAAA TTGAGAAAAT TCGAGCAAGT TTATTTAACA ACAATGACTT GATAGGTTTG
    TCAAGCTTGG ATGGTGAGGA TGAATTGATG GAAATGTCAA CGGAAGAGAT CCTCACGGTG
    TCTGTAGTGA ATCAGAGTCT ATTCGATACG CAGGGGAGCC CAGGGTTAGA AGATTATTTC
    AATGATAAGT CAATTAAAGG TGAGAAGCTG GTGCCTGGGG CCAGAGAGGT GCTGACGGAG
    ATATTTAAGA GCTGTGCCCA CTCGGAGCAG ATGCTGAGCC TGACGCCAGC AAAGCCCATC
    AAAGTGTCGG ACATTTATCT TAGCAAAGAG CAGATCAACT CCCAGACACC GGGCAACCTC
    CTCCACCTTT TCTTCACCAA TGTCCGGCCC CCCAAAAAGG TGCTAGAGGA CCAGCTCACC
    CAGATCCTGA GGAAGTACGG TGTGCCGAAG CCCAAGTTTG ACAAGAGCAA GTACAGCAAG
    GCGGGGAAGG AGCAGCACCC CGGGAAGGTG GTGAGCACCA AGCGGCCCAT CACCAAGCCG
    CCCACCAAGG ACAAGGCAGT GCTCAACAGC GTCAGCAGGA CTGCCTTGAG TGAGAAGAAG
    CCCACCGTGA AGCCCAAGTC ACCTGAAAAG AGCAAGCCAG ATGAAAAGGA CCCTGAAAAG
    TCACCCACCA AGAAACAAGA CGTCCCAGAG GAGAAGTACC TGACCTTAGA AGGATTTCAC
    AAGTTTGTGA TCGACCGAGC CAAGCAAGAC ATCCGCAGCG TCTGGAGAGC GATTTTATCC
    TGTGGCTACG ATCTTCATTT TGAGAGGTGT GCCTGCATCG ACGTCCGGCA TGCGCACAAG
    GCCTCCAGGA AGTGGACCCT GGAGATGGAT GTGGCGCTCG TGCAGTACAT CAACCGGCTG
    TGCCGCCACC TGGCCATCAC GCCCGCGCGT CTGCATCCCC ACGAGGTGTA TCTGGACCCT
    GCAGACGCTG CTGACCCCAG AGTGGCCTGT CTCTTGAACG TGCCCATCGA GAGCCTGCGC
    CTGCGCTTCG CTCTGCTGCA GTCCCTCAAC ACCACGCTCG AGACCTTCTT CCTGCCGCTG
    GTTGAGCTGC GCCAGACTCC CATGTACACG CACAGCATCG CCGCCCTGCT CAAGGAGGCC
    AAAGGGCTGA TCTTTTATGA CACAAAGGTG ACCGTCATGA ATCGGGTGCT AAATGCCACG
    GTGCAGAGGA CGGCTGACCA TGCAGCACCA GAAATCACTC TGGACCCGCT GGAGATCGTG
    GGAGGGGAGA TCAGAGCCTC TGAGAACTCC TACTTCTGCC AGGCAGCCAG GCAGCTGGCC
    TCAGTGCCCT CCTCCCAGCT TTGCGTCAAA CTGGCGAGTG GCGGCGACCC TACCTATGCC
    TTCAACATCC GCTTCACGGG AGAGGAGGTC CATGGCACCA GTGGCTCCTT CCGCCACTTC
    CTGTGGCAGG TGTGCAGAGA GCTGCAGAGC TCCTCGCTCT CCCTCCTCCT GCTGTGCCCC
    AGCTCCGCCG TCAACAAGAA CAAGGGCAAG TACATCCTGA CCCCCAGCCC CATCACCTAT
    GGGGAGGAGC AGCTGCTGCA CTTCCTGGGC CAGTTGCTAG GGATCGCCAT CCGAGCAGAT
    GTCCCCCTCC CCCTGGACCT CCTGCCCTCC TTCTGGAAGA CACTGGTGGG CGAGCCCCTG
    GACCCTGACC AAGACCTGCA GGAAGCAGAC GTACTCACCT ACAATTACGT CAAGAAATTT
    GAGAGTATCA ACGACGAGAC GGAGTTGGAG GCCCTGTGTG CTGAGATCGC CTCCCAGCAC
    CTGGCCACTG AGAGCCCCGA GGGGCCCAAC AAGCCCTGCT GCCGGTTCAC CTACCTGACC
    ATGACCGGCG AGGAGGTGGA GCTGTGCAGC CGTGGCCGGC ACATCCCCGT GGCGTGGGAG
    AACAAGGACA TCTATGCGGC CGCCATCCGG AGCCTGCGGC TGCGTGAGCT ACAGAACGTG
    GAGTGTGTGA CGGCCGTGCG CGCGGGCCTT GGGGCCATCA TCCCCCTGCA GCTGCTCACC
    ATGCTCAGCC CACTGGAGAT GGAGCTGCGC ACCTGCGGCC TCCCGTACAT CAACCTCGAG
    TTCCTGAAGG CCCACACCAT GTACCAGGTG GGGCTGATGG AGACCGACCA GCACATCGAG
    TTCTTCTGGG GGGCACTGGA GATGTTTGCC CAGGAGGAGC TGTGCAAGTT CATCAAGTTT
    GCCTGCAACC AGGAGCGCAT CCCGTTCACC TGCCCCTGCA AAGACGGGGG TCCTGACACG
    GCCCATGTGC CCCCATACCC CATGAAGATT GCCCCCCCAG ATGGCACAGC AGGTTCCCCA
    GATTCTCGCT ACATCCGAGT GGAGACCTGT ATGTTCATGA TCAAGCTTCC CCAGTACTCC
    TCGCTGGAAA TCATGCTGGA GAAACTACGT TGTGCCATTC ACTACCGCGA AGACCCTCTG
    AGTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.