RASAL3 cDNA ORF clone, Gallus gallus(chicken)

The following RASAL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RASAL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa149226 XM_025145736.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa149227 XM_025145737.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa149224 XM_025145731.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa149225 XM_025145732.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa149225 XM_025145733.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa149225 XM_025145735.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OGa149219 XM_025145727.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa149220 XM_025145728.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa149221 XM_025145729.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa149222 XM_025145730.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa149223 XM_015302685.2
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OGa49766 XM_015302685.1
Latest version!
Gallus gallus RAS protein activator like 3 (RASAL3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa149226
    Clone ID Related Accession (Same CDS sequence) XM_025145736.1
    Accession Version XM_025145736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
    CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGGT
    GAGCGGCCCC TCCCCCCGGC CCCGCCGCCT TCTTCTCCCC GTCGCTCTCG GCCCCACCGC
    GGGGCCCGGC CCGGGGCTGA GGGAGCGCGG GGCCATTCCC GGCGCCGCGC CGCGCTGTGG
    GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA
    CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC
    ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA
    ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001504.1
    CDS39..2810
    Translation

    Target ORF information:

    RefSeq Version XM_025145736.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
    CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGGT
    GAGCGGCCCC TCCCCCCGGC CCCGCCGCCT TCTTCTCCCC GTCGCTCTCG GCCCCACCGC
    GGGGCCCGGC CCGGGGCTGA GGGAGCGCGG GGCCATTCCC GGCGCCGCGC CGCGCTGTGG
    GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA
    CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC
    ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA
    ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149227
    Clone ID Related Accession (Same CDS sequence) XM_025145737.1
    Accession Version XM_025145737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
    CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGTT
    CTGGCCCCGG AGGAGGAGGA GGTGGTGGCC ATGGAGGTGG GTTCCCCCCC GCCTCCACCA
    CCGCCACCGC TGCCCAAGAA GAGCTCAGCC CCGGGGCAGT CGGAGCAGCC CGCCCGCATA
    GGGACCAAGC TGTGCGCCGA GCCGCCCGGC GGCAAAGCGG ATCCACACGA CCCCAAAACC
    CAGAACCTGA CGACGTGCGA GGTGTGCGGC GCCTGCTTCG AAACCCGCAA AGGGCTCTCC
    AGCCACGCGC GCTCCCACCT GCGGCAGCTC GGCGTGGCCG AGTCGGAGAG CAGCGGGGCC
    CCCATCGATT TGCTCTACGA ACTGATGAAG CAGAAGGGCA AACCCGATGG GCACCCCATG
    GCTCCCCCCA CCCTCAGCAA AAAATCCGCT TCTCCCAAGG ACGGCATTGC CGGCTCCCCG
    CGGCCCGCGC TGCTCGCGCT CGGCAAAGGC GGTGAGCGCC CGCTGGATGG CCCCATCAAC
    AAAGCCATCA AATCCCCGCC AGGATTCTCC AAGAGCCTCT CGCAGCCCGG ATCCCCCATC
    CTCAAGAAGG TGCCCCCCGC CCTCTCCGGG TCCCCCTCTC CGAAAAACCC CGAGGAGAAG
    AGCTCGAAGC TATCGCTGAG CCCTCTGCAG AGCTCCCCAA AGGCGCAGTG GCCGCAGGCG
    GATGAGGAAG GACCTCTCAA TTTGACCGCG GGGGGAGAGC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTAC GTCAACGGCT CACCCATCGA CACGCTGCGC
    GAGATCCTCA AGCGCCGAAC CCACCCGCGG AGCGGAACCT CCAACCCCAT GGGCCCCGGG
    CACAAAGCCA TGGCCAAGAC CCTCCTGGCA GCCATGGGAC CCCTGGAGCC GCGGGGTCCC
    GGGGAGCTCC ACATCCCGAC TCTGCCCAAG AAGGTCCAGC AGCCTGGCAA TCCCCTGGGG
    CAGTCTCCTA CCTCGTCCCC ACCTCCCACT GCCCGGAAGA TGTTTCCAGG CCTCTCTCCT
    CCCTCCTTGC AGAAGAAGCT CAAACATGAC CAGATGAGAG TGGAAATCAA GCGTGAGATG
    ATGACGGGGG GTCTCCACGG AGAGCCCCAC CCCTCCGACC AGGCTTGGCC ACCGCTGGAG
    GAGATGTCAC CCCTGAACCT CTCCTCCCGG GCCGACCCGG TGCGGGACAT CCGCTGCGAG
    TTCTGCGGAG AGTTCTTTGA GAACCGCAAG GGGCTGTCGA GCCACGCGCG CTCCCACCTC
    CGACAGATGG GGGTGACGGA GTGGTCGGTG AACGGCTCTC CCATCGACAC GCTGCGCGAG
    ATCCTCAAGA AGAAATCGAA GCCCTGCGTC ATCAAGAAGG AGCCCCACAC CTCCATCATT
    GAAACCCCCA AAGGGTTTGG GGAGGAGGGG ATGGACCCCA AGCCCCCCGG AAAAGTGCTG
    CAGGCCATGG CTCTCCCTCC GCTGGGCGGG CGGACGGGGA AACCCGGTGG CTCCAGCATG
    GGCCGCGAGC TCTCGCTGTC GCCGCTCACC ACCAAGCCCC ACGGTGCCTT CTTGACACCA
    CTGGCCACCA AACGGCCACT GCAGGACGAC CGCCTGGGTC CCCACCCCGA GGTGAAGCAC
    AAGGCCTACA TCCAGACCGA GCTGCCCTTC AAAACCAAGT CCGTACACGA CAAGCCCACG
    CACACATCCA CCGAAGCGTG CTGCGAGCTC TGCGGCCTCT ACTTCGAGAA CCGCAAGGCT
    CTGGCCAGCC ACGCGCGGGC GCACCTCCGG CAATTCGGCG TCACCGAATG GTGCGTCAAC
    GGCTCTCCCA TCGAGACGCT CAGCGAATGG ATCCGGCACC GGCCGCAGAA AGCCGGCGCG
    TACCGCAGCT ACATCCAGGG GGGCCGACCC TTCACAAAGA AGTTCAGGAA TTCTTCGCAC
    GCCCGAGACC ACGACGGAGC GCGGAGGATG CCGCTGAGCC TGCAGCACGG CGGCATGGCC
    CTGCTGAGCA AAGGGCTGGC GGCGGAGCTG GCGCACGGGG AGCCCTGCAA GATCCTGGAC
    GGCACCGGGG AGCGGCCCAT GGTCACCTCG CCCCTCTCGC TGGTGAAGAT GGAAGAACAT
    CAGCGCACGA ATTTCCACAA GTTCGAGCGG AGGCAGGCAA GGCCCCTGGA TCCCACCCTG
    CACAGGGAAG AGGAGGGGGC CGACTTCCAG CAGAAGATGG AGGAGACCCG CCAGCCCCCC
    CCCAGGATGA GGCCGGTGCC CTCGCTGGTC CCCCGCCCGC CACAGACCTC CCTGGTGAAG
    TTTGTGGGTA ACATCTACAC CCTCAAGTGC AGGTTCTGTG AGGTGGAGTT CCAGGGCCCT
    CTCTCCATCC AGGAGGAGTG GGTGCGACAC CTCCAGCGAC ACATCCTGGA AATGAATTTC
    TCCAAAGCAG ACCCCCTGCG GGGGGACGCC CCGGCCCCCG AACCCCCCGC CGTGGCCGAG
    GCTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001505.1
    CDS39..2687
    Translation

    Target ORF information:

    RefSeq Version XM_025145737.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
    CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGTT
    CTGGCCCCGG AGGAGGAGGA GGTGGTGGCC ATGGAGGTGG GTTCCCCCCC GCCTCCACCA
    CCGCCACCGC TGCCCAAGAA GAGCTCAGCC CCGGGGCAGT CGGAGCAGCC CGCCCGCATA
    GGGACCAAGC TGTGCGCCGA GCCGCCCGGC GGCAAAGCGG ATCCACACGA CCCCAAAACC
    CAGAACCTGA CGACGTGCGA GGTGTGCGGC GCCTGCTTCG AAACCCGCAA AGGGCTCTCC
    AGCCACGCGC GCTCCCACCT GCGGCAGCTC GGCGTGGCCG AGTCGGAGAG CAGCGGGGCC
    CCCATCGATT TGCTCTACGA ACTGATGAAG CAGAAGGGCA AACCCGATGG GCACCCCATG
    GCTCCCCCCA CCCTCAGCAA AAAATCCGCT TCTCCCAAGG ACGGCATTGC CGGCTCCCCG
    CGGCCCGCGC TGCTCGCGCT CGGCAAAGGC GGTGAGCGCC CGCTGGATGG CCCCATCAAC
    AAAGCCATCA AATCCCCGCC AGGATTCTCC AAGAGCCTCT CGCAGCCCGG ATCCCCCATC
    CTCAAGAAGG TGCCCCCCGC CCTCTCCGGG TCCCCCTCTC CGAAAAACCC CGAGGAGAAG
    AGCTCGAAGC TATCGCTGAG CCCTCTGCAG AGCTCCCCAA AGGCGCAGTG GCCGCAGGCG
    GATGAGGAAG GACCTCTCAA TTTGACCGCG GGGGGAGAGC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTAC GTCAACGGCT CACCCATCGA CACGCTGCGC
    GAGATCCTCA AGCGCCGAAC CCACCCGCGG AGCGGAACCT CCAACCCCAT GGGCCCCGGG
    CACAAAGCCA TGGCCAAGAC CCTCCTGGCA GCCATGGGAC CCCTGGAGCC GCGGGGTCCC
    GGGGAGCTCC ACATCCCGAC TCTGCCCAAG AAGGTCCAGC AGCCTGGCAA TCCCCTGGGG
    CAGTCTCCTA CCTCGTCCCC ACCTCCCACT GCCCGGAAGA TGTTTCCAGG CCTCTCTCCT
    CCCTCCTTGC AGAAGAAGCT CAAACATGAC CAGATGAGAG TGGAAATCAA GCGTGAGATG
    ATGACGGGGG GTCTCCACGG AGAGCCCCAC CCCTCCGACC AGGCTTGGCC ACCGCTGGAG
    GAGATGTCAC CCCTGAACCT CTCCTCCCGG GCCGACCCGG TGCGGGACAT CCGCTGCGAG
    TTCTGCGGAG AGTTCTTTGA GAACCGCAAG GGGCTGTCGA GCCACGCGCG CTCCCACCTC
    CGACAGATGG GGGTGACGGA GTGGTCGGTG AACGGCTCTC CCATCGACAC GCTGCGCGAG
    ATCCTCAAGA AGAAATCGAA GCCCTGCGTC ATCAAGAAGG AGCCCCACAC CTCCATCATT
    GAAACCCCCA AAGGGTTTGG GGAGGAGGGG ATGGACCCCA AGCCCCCCGG AAAAGTGCTG
    CAGGCCATGG CTCTCCCTCC GCTGGGCGGG CGGACGGGGA AACCCGGTGG CTCCAGCATG
    GGCCGCGAGC TCTCGCTGTC GCCGCTCACC ACCAAGCCCC ACGGTGCCTT CTTGACACCA
    CTGGCCACCA AACGGCCACT GCAGGACGAC CGCCTGGGTC CCCACCCCGA GGTGAAGCAC
    AAGGCCTACA TCCAGACCGA GCTGCCCTTC AAAACCAAGT CCGTACACGA CAAGCCCACG
    CACACATCCA CCGAAGCGTG CTGCGAGCTC TGCGGCCTCT ACTTCGAGAA CCGCAAGGCT
    CTGGCCAGCC ACGCGCGGGC GCACCTCCGG CAATTCGGCG TCACCGAATG GTGCGTCAAC
    GGCTCTCCCA TCGAGACGCT CAGCGAATGG ATCCGGCACC GGCCGCAGAA AGCCGGCGCG
    TACCGCAGCT ACATCCAGGG GGGCCGACCC TTCACAAAGA AGTTCAGGAA TTCTTCGCAC
    GCCCGAGACC ACGACGGAGC GCGGAGGATG CCGCTGAGCC TGCAGCACGG CGGCATGGCC
    CTGCTGAGCA AAGGGCTGGC GGCGGAGCTG GCGCACGGGG AGCCCTGCAA GATCCTGGAC
    GGCACCGGGG AGCGGCCCAT GGTCACCTCG CCCCTCTCGC TGGTGAAGAT GGAAGAACAT
    CAGCGCACGA ATTTCCACAA GTTCGAGCGG AGGCAGGCAA GGCCCCTGGA TCCCACCCTG
    CACAGGGAAG AGGAGGGGGC CGACTTCCAG CAGAAGATGG AGGAGACCCG CCAGCCCCCC
    CCCAGGATGA GGCCGGTGCC CTCGCTGGTC CCCCGCCCGC CACAGACCTC CCTGGTGAAG
    TTTGTGGGTA ACATCTACAC CCTCAAGTGC AGGTTCTGTG AGGTGGAGTT CCAGGGCCCT
    CTCTCCATCC AGGAGGAGTG GGTGCGACAC CTCCAGCGAC ACATCCTGGA AATGAATTTC
    TCCAAAGCAG ACCCCCTGCG GGGGGACGCC CCGGCCCCCG AACCCCCCGC CGTGGCCGAG
    GCTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149224
    Clone ID Related Accession (Same CDS sequence) XM_025145731.1
    Accession Version XM_025145731.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5718bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001499.1
    CDS16..5733
    Translation

    Target ORF information:

    RefSeq Version XM_025145731.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145731.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149225
    Clone ID Related Accession (Same CDS sequence) XM_025145733.1 , XM_025145735.1 , XM_025145732.1
    Accession Version XM_025145733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001501.1
    CDS254..5551
    Translation

    Target ORF information:

    RefSeq Version XM_025145733.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149225
    Clone ID Related Accession (Same CDS sequence) XM_025145733.1 , XM_025145735.1 , XM_025145732.1
    Accession Version XM_025145735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001503.1
    CDS224..5521
    Translation

    Target ORF information:

    RefSeq Version XM_025145735.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149225
    Clone ID Related Accession (Same CDS sequence) XM_025145733.1 , XM_025145735.1 , XM_025145732.1
    Accession Version XM_025145732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001500.1
    CDS8350..13647
    Translation

    Target ORF information:

    RefSeq Version XM_025145732.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
    GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG
    GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG
    AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG
    GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA
    GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT
    TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA
    GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG
    GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC
    TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC
    GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC
    AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG
    GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG
    GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC
    ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC
    AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC
    TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC
    CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC
    GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG
    ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC
    TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC
    TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC
    AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG
    AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA
    CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT
    TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC
    GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA
    AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC
    AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA
    AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG
    GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG
    GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC
    ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC
    CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG
    CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG
    AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG
    CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG
    AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG
    GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC
    ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA
    AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG
    GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT
    GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC
    AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC
    CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT
    CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC
    AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG
    CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC
    GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC
    CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA
    GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC
    AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG
    CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC
    GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC
    CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA
    TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC
    GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG
    CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC
    ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG
    CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC
    ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG
    GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG
    CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT
    CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC
    CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG
    CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA
    CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG
    CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC
    TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA
    AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC
    TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC
    TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG
    GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC
    AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC
    CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG
    TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA
    GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT
    TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC
    GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG
    ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG
    GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT
    CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC
    CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC
    CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC
    CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA
    ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC
    GTGGCCGAGG CTCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149219
    Clone ID Related Accession (Same CDS sequence) XM_025145727.1
    Accession Version XM_025145727.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6255bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC
    CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC
    TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT
    GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG
    CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG
    CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC
    ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA
    GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC
    AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG
    ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG
    AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG
    CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC
    AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC
    GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC
    GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT
    TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC
    ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC
    CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA
    GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC
    ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG
    CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG
    GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG
    GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG
    AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG
    GCCGAGGCTC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001495.1
    CDS16..6270
    Translation

    Target ORF information:

    RefSeq Version XM_025145727.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145727.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC
    CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC
    TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT
    GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG
    CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG
    CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC
    ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA
    GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC
    AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG
    ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG
    AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG
    CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC
    AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC
    GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC
    GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT
    TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC
    ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC
    CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA
    GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC
    ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG
    CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG
    GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG
    GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG
    AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG
    GCCGAGGCTC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149220
    Clone ID Related Accession (Same CDS sequence) XM_025145728.1
    Accession Version XM_025145728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6252bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC
    TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC
    ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG
    GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG
    CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC
    TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC
    GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA
    CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC
    ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA
    ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001496.1
    CDS16..6267
    Translation

    Target ORF information:

    RefSeq Version XM_025145728.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC
    TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC
    ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG
    GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG
    CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC
    TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC
    GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA
    CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC
    ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA
    ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149221
    Clone ID Related Accession (Same CDS sequence) XM_025145729.1
    Accession Version XM_025145729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGA AGAAGCTCAA ACATGACCAG
    ATGAGAGTGG AAATCAAGCG TGAGATGATG ACGGGGGGTC TCCACGGAGA GCCCCACCCC
    TCCGACCAGG CTTGGCCACC GCTGGAGGAG ATGTCACCCC TGAACCTCTC CTCCCGGGCC
    GACCCGGTGC GGGACATCCG CTGCGAGTTC TGCGGAGAGT TCTTTGAGAA CCGCAAGGGG
    CTGTCGAGCC ACGCGCGCTC CCACCTCCGA CAGATGGGGG TGACGGAGTG GTCGGTGAAC
    GGCTCTCCCA TCGACACGCT GCGCGAGATC CTCAAGAAGA AATCGAAGCC CTGCGTCATC
    AAGAAGGAGC CCCACACCTC CATCATTGAA ACCCCCAAAG GGTTTGGGGA GGAGGGGATG
    GACCCCAAGC CCCCCGGAAA AGTGCTGCAG GCCATGGCTC TCCCTCCGCT GGGCGGGCGG
    ACGGGGAAAC CCGGTGGCTC CAGCATGGGC CGCGAGCTCT CGCTGTCGCC GCTCACCACC
    AAGCCCCACG GTGCCTTCTT GACACCACTG GCCACCAAAC GGCCACTGCA GGACGACCGC
    CTGGGTCCCC ACCCCGAGGT GAAGCACAAG GCCTACATCC AGACCGAGCT GCCCTTCAAA
    ACCAAGTCCG TACACGACAA GCCCACGCAC ACATCCACCG AAGCGTGCTG CGAGCTCTGC
    GGCCTCTACT TCGAGAACCG CAAGGCTCTG GCCAGCCACG CGCGGGCGCA CCTCCGGCAA
    TTCGGCGTCA CCGAATGGTG CGTCAACGGC TCTCCCATCG AGACGCTCAG CGAATGGATC
    CGGCACCGGC CGCAGAAAGC CGGCGCGTAC CGCAGCTACA TCCAGGGGGG CCGACCCTTC
    ACAAAGAAGT TCAGGAATTC TTCGCACGCC CGAGACCACG ACGGAGCGCG GAGGATGCCG
    CTGAGCCTGC AGCACGGCGG CATGGCCCTG CTGAGCAAAG GGCTGGCGGC GGAGCTGGCG
    CACGGGGAGC CCTGCAAGAT CCTGGACGGC ACCGGGGAGC GGCCCATGGT CACCTCGCCC
    CTCTCGCTGG TGAAGATGGA AGAACATCAG CGCACGAATT TCCACAAGTT CGAGCGGAGG
    CAGGCAAGGC CCCTGGATCC CACCCTGCAC AGGGAAGAGG AGGGGGCCGA CTTCCAGCAG
    AAGATGGAGG AGACCCGCCA GCCCCCCCCC AGGATGAGGC CGGTGCCCTC GCTGGTCCCC
    CGCCCGCCAC AGACCTCCCT GGTGAAGTTT GTGGGTAACA TCTACACCCT CAAGTGCAGG
    TTCTGTGAGG TGGAGTTCCA GGGCCCTCTC TCCATCCAGG AGGAGTGGGT GCGACACCTC
    CAGCGACACA TCCTGGAAAT GAATTTCTCC AAAGCAGACC CCCTGCGGGG GGACGCCCCG
    GCCCCCGAAC CCCCCGCCGT GGCCGAGGCT CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001497.1
    CDS16..6171
    Translation

    Target ORF information:

    RefSeq Version XM_025145729.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT
    CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC
    CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC
    AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGA AGAAGCTCAA ACATGACCAG
    ATGAGAGTGG AAATCAAGCG TGAGATGATG ACGGGGGGTC TCCACGGAGA GCCCCACCCC
    TCCGACCAGG CTTGGCCACC GCTGGAGGAG ATGTCACCCC TGAACCTCTC CTCCCGGGCC
    GACCCGGTGC GGGACATCCG CTGCGAGTTC TGCGGAGAGT TCTTTGAGAA CCGCAAGGGG
    CTGTCGAGCC ACGCGCGCTC CCACCTCCGA CAGATGGGGG TGACGGAGTG GTCGGTGAAC
    GGCTCTCCCA TCGACACGCT GCGCGAGATC CTCAAGAAGA AATCGAAGCC CTGCGTCATC
    AAGAAGGAGC CCCACACCTC CATCATTGAA ACCCCCAAAG GGTTTGGGGA GGAGGGGATG
    GACCCCAAGC CCCCCGGAAA AGTGCTGCAG GCCATGGCTC TCCCTCCGCT GGGCGGGCGG
    ACGGGGAAAC CCGGTGGCTC CAGCATGGGC CGCGAGCTCT CGCTGTCGCC GCTCACCACC
    AAGCCCCACG GTGCCTTCTT GACACCACTG GCCACCAAAC GGCCACTGCA GGACGACCGC
    CTGGGTCCCC ACCCCGAGGT GAAGCACAAG GCCTACATCC AGACCGAGCT GCCCTTCAAA
    ACCAAGTCCG TACACGACAA GCCCACGCAC ACATCCACCG AAGCGTGCTG CGAGCTCTGC
    GGCCTCTACT TCGAGAACCG CAAGGCTCTG GCCAGCCACG CGCGGGCGCA CCTCCGGCAA
    TTCGGCGTCA CCGAATGGTG CGTCAACGGC TCTCCCATCG AGACGCTCAG CGAATGGATC
    CGGCACCGGC CGCAGAAAGC CGGCGCGTAC CGCAGCTACA TCCAGGGGGG CCGACCCTTC
    ACAAAGAAGT TCAGGAATTC TTCGCACGCC CGAGACCACG ACGGAGCGCG GAGGATGCCG
    CTGAGCCTGC AGCACGGCGG CATGGCCCTG CTGAGCAAAG GGCTGGCGGC GGAGCTGGCG
    CACGGGGAGC CCTGCAAGAT CCTGGACGGC ACCGGGGAGC GGCCCATGGT CACCTCGCCC
    CTCTCGCTGG TGAAGATGGA AGAACATCAG CGCACGAATT TCCACAAGTT CGAGCGGAGG
    CAGGCAAGGC CCCTGGATCC CACCCTGCAC AGGGAAGAGG AGGGGGCCGA CTTCCAGCAG
    AAGATGGAGG AGACCCGCCA GCCCCCCCCC AGGATGAGGC CGGTGCCCTC GCTGGTCCCC
    CGCCCGCCAC AGACCTCCCT GGTGAAGTTT GTGGGTAACA TCTACACCCT CAAGTGCAGG
    TTCTGTGAGG TGGAGTTCCA GGGCCCTCTC TCCATCCAGG AGGAGTGGGT GCGACACCTC
    CAGCGACACA TCCTGGAAAT GAATTTCTCC AAAGCAGACC CCCTGCGGGG GGACGCCCCG
    GCCCCCGAAC CCCCCGCCGT GGCCGAGGCT CAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149222
    Clone ID Related Accession (Same CDS sequence) XM_025145730.1
    Accession Version XM_025145730.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6075bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGCCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC
    CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC
    TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT
    GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG
    CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG
    CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC
    ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA
    GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC
    AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG
    ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG
    AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG
    CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC
    AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC
    GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC
    GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT
    TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC
    ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC
    CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA
    GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC
    ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG
    CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG
    GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG
    GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG
    AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG
    GCCGAGGCTC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025001498.1
    CDS16..6090
    Translation

    Target ORF information:

    RefSeq Version XM_025145730.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025145730.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC
    GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG
    ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC
    TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA
    GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA
    CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA
    GACGCCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG
    CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC
    GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC
    CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC
    TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC
    ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC
    CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG
    GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG
    CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC
    CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC
    TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG
    CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC
    CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG
    GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC
    CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC
    TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT
    GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG
    CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG
    CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC
    ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA
    GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC
    AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG
    ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG
    AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG
    CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC
    AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC
    GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC
    GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT
    TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC
    ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC
    CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA
    GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC
    ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG
    CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG
    GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG
    GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG
    AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG
    GCCGAGGCTC AGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa149223
    Clone ID Related Accession (Same CDS sequence) XM_015302685.2
    Accession Version XM_015302685.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6072bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product protein Wiz isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015302685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC
    TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC
    ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG
    GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG
    CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC
    TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC
    GCCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015158171.2
    CDS16..6087
    Translation

    Target ORF information:

    RefSeq Version XM_015302685.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015302685.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
    CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG
    GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG
    GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT
    TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG
    GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG
    CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG
    CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC
    CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT
    GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG
    CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA
    GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC
    GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA
    GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC
    GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA
    GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG
    ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC
    AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG
    TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC
    GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG
    CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC
    GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT
    CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA
    ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA
    GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG
    TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA
    GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG
    CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG
    CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG
    GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC
    GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC
    CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC
    AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC
    CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG
    GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC
    TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG
    AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC
    AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC
    ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA
    TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG
    GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT
    TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC
    ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG
    GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA
    AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA
    AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC
    CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG
    TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC
    CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT
    TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG
    GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG
    AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC
    CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG
    AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG
    AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC
    TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA
    GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC
    TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC
    ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG
    GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG
    CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC
    TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC
    GCCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC
    TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG
    GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC
    ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC
    CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC
    AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC
    ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG
    AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG
    GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC
    TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC
    CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG
    CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC
    GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT
    CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG
    GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT
    CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG
    ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG
    GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC
    GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC
    CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC
    GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC
    ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG
    CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC
    ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA
    CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG
    CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC
    ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG
    GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC
    AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC
    GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG
    CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG
    GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG
    GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA
    CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC
    CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC
    CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG
    AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC
    CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT
    TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC
    GAGGCTCAGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa49766
    Clone ID Related Accession (Same CDS sequence) XM_015302685.1
    Accession Version XM_015302685.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product LOW QUALITY PROTEIN: protein Wiz
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_463422.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAAAG CTGTCCCTAC TTTATCAGAG CAACAGCGAG AGCGCCAGGG AGATGGAAGC 
    AGAGCCCTGC TAATGCCACT GGAGCCAAAC AAAGAGAATT TTGCTGCCCC TGATTGCCCC
    CAAGGTGAAG AGAGAAGAGC TGCTTTTGTG AAAGATGCAA AAAGGCCTCT GGAGAGCGCG
    GCTCTATCTG GGACATCGGT GGAAGTGGGG ACGGAGCTCG ACGAAGCCGC CTTCTACACC
    GCTGATGACT TGGTGACAAT GAGCAGGGAC ACAAAGGGAC CCTCCGGCAC CGATCTCTCC
    CCCCTACAAG AGGACTCCGT CTTCTTCTCT CCGCTGTCCC GCCAAGAGCC CGACATTGCC
    CTGGAAGCCT GCGACGTGGC CGATGAGCCT GATCCTGCCC TCTCCGGTGC TGTCCCAGCC
    CCAGCTCACA GGACCGAACT CGGCTCCAAG ACCGAAGAGC ATTTAGAGTT CCAGAAGGCT
    TTGTACAGAG CGGACGCCAG GAGCTCCCTG GAGCTGCGCG CCATGACCGT GTCCGGCAGC
    GTGCCCTGGG ACACCTCCGC CCTGGAGACC CGAGCGGGTG ATGGCAGAGA AGAAAGCACT
    TTAGAGAGAC TCTCTAGCTT GAAGGACTCA AAGGACGTGT CGGCGTTCCC GGGGGATCCC
    CAGGTTGTAA ATACGGTGCG GGTGGATCCT GAGGACCTGG AGAGCGACGC TGAGGAGCTG
    GGCGCCGGCA TGGCGGCAGC TGGGACGCCC AACCCTTGCA CGGGGCCACC GGTGGGTAGT
    GGGGAGGAGA AATACCTTAA ACCTGAACCA AAGCAACTTG AAGGCGTCGG CAAAGAGCAC
    TTAGAGAAAA GCAGGAGGGG GATCCAGAGG GTCGACTGGA TTTCTCGACC CAACAGAAGC
    CCGAGTCCTC ACTACAAAGA CGTGAGCAAG CCGACGGATG TAGCAACCAA CGTACCCTTC
    CGGGGACAGG CTGTGCGTGA AGTGCCTCCT CCGCTGACTC CGGCAGTGTT CAGGAAATCT
    CTGAACCCTG TCCTCAGCAA GTCCAAAACC CTCGAGACCC CCTCGTCCCA CAGGAAGGGG
    CTGGGGGTCG ACTCGGACGC GGGTGAGATG GGCGCGCTCA GCGCAGCTGA GTGGACCATC
    CCAAAGCCGG CCCTGCAGCT CAGCACCGAC CTCGCCATCG GCAAGCAGTC GTGGACGGTG
    AATTCGGAGG ATTCGGTGGA GAGGATCCCG TTGATGTCCC TGGAGCCCAC TCCCTGCAGC
    TCCTATGACG TGGAGATGAG GCCCTACGTG TGCAGCGTGC TGGTGGAGGA GCAGGGCAAA
    GAGGAGCTGG GCCCCGAGGA GGACCCCACG GTGTACACGT GCATCGAGTG CAGCATCTAC
    TTCAAGAAGA AGGAGCACCT GATGGATCAC ATGCTCCAGC ACAACCGTGG ACCAGGGAGG
    GACCAAGATG GGGACGCTCT CGGAGGGCAG TGCCAGTTCT GCTGCAGTGA GTGCGGGTGG
    GCGTTTGGGG ACCTTGCATC CCTAGAGCAG CACAAAAGGC TCCACCAGGA ATCCAGGGAA
    AAAATCATTG AGGAGATCCA GAAGCTGAAC GAGTTCCCTG ACGAGGGTCG GGAAGCCCGG
    CTGCAGTGCC CCAAGTGCAT CTTTGGCACC AACTCCTCCA AGATCTTCGT GCAGCACGCC
    AAGATGCATG TCAAGGAGAG GAAGGACCAA GGGGCGAAGA GCTTGAATCT CTACGGGGGC
    GGAGAGCTGC AGGATAGCCC CGTGCACGGT ATGTACAAAC ACTTCAAACC CAATGAGCAC
    ATGCCCCTGC AAGTGCAGGC GCCTCCGCAC GGCAAAGGGC TCAGCACCTG CATGCTCTGC
    AGCTTCCCGG CGCCCAACGA GAACATCCTC AAAGAGCACA TGAAATACGC CCATTCCCAC
    CTCTCTTGGG ACGCGGAGGT GTACGAAGAA GATCCCAACC AACCGGGAAC CAGCAGGGAC
    GCCTACAGCC CCGCCAGGCC GGGCCGCTTT GCAGAGACTG ATTATTTTGG CAAAGCAGAC
    CAGCTCTTCC CTCCACCCCA CCGGGAAAGC GCGTCGCATT ACGACATCCA CAGCTTCACC
    GTAGATCACC CCAGACTCAA CAGAAGCAAT GGGGGTAGCA AAAAGGACTA CTCGACGTCG
    GGGTTCCATG CCAGGAAAGC GGCCCCGTAC GCTGCCCGGC ACAAAAACCT GGGGCTCTCC
    TCCACGAAGG CTTATTCACA GTTTGCTCTG CAGCACCTGA AAAAAAAAAC AGCAGCTCAG
    CACCTCGAAG CCGAAGGCGA CGGTCTGAGG GGTCACCCAA CGGGCCTGGA GGAGGTCGGG
    CACAAATGGA TGTTTATCAA CGAGGTGGGG GGCGTGGAAG GGGAGTTCTC CATGACCACG
    GACATGGACT TGATGGAGCA CAGAGTCACC AAACCCGTCA CCATCCCTCA AGCTGCTTTG
    GACCTCAAGA GGACGTACAG AGACACGTTG AGAGCCATGG ACCCTTTGGT CGCCTCCGAA
    GAGCAGCAGC AGCAGCTGCG GATGATGGTG CCCATCGTTC TGCTGGAGGA GGTCAACCCA
    CACCCCAAAG CAAAGAAGCG GCCCCGGGCA AAGCCCTGCA AGAAGAAAGC AGCATTCCCA
    TCGCGGGAGT TCATGATGGA AGAGCCTCTT CCCTTGGATG TGCTCCTGTT GGACTCTCCT
    TTAGAGTTTG ATGACTTTTT GGACTCCGAC TCGCCCATGT TGAAGAACGA GGAGAGGAAG
    TGCCCGTATT GCCCGGATCG GTTCCACAAC GGCATTGGGT TGGCGAACCA CGTGCGGGGC
    CACCTCAATC GGGTTGGTGT GAGCTACAAC GTCCGGCATT TCATCTCGGC AGAAGAAGTG
    AAAGCTATTG AGCAAAAATT TTCCTTCCAA AAGAAGAAGA AAAAAGTTGC AAACTTCGAC
    CCGAGCACGT TCAGCCTGAT GCGATGCGAG TTCTGCGGGG CCGGCTTCGA CACACGCGCC
    GGGCTCTCCA GCCACGCCAG GGCCCACCTG AGAGACTTTG GGATCACCAA CTGGGAGCTC
    ACCATCTCGC CCATCAACAT CCTCAAGGAG CTGTTGGCCA ACTCGGCGGA GTACCCCATG
    CTGCAGGCGG CGGTGGGAGC GGAGCCCTCA TCCCCGGGCC GAGAGCCGCA CGGCTTCGTG
    CCCCGCAAGA GCATGACCCC CATCTCCGAG TGCAGCATTC CACGCTCTCC TCTGTCCCCA
    TTCCCTCCAT CTTGGGGAGA TGAGTCCTTG CAGTCCTACA GAGACGCCCA GAACCTGACG
    ACGTGCGAGG TGTGCGGCGC CTGCTTCGAA ACCCGCAAAG GGCTCTCCAG CCACGCGCGC
    TCCCACCTGC GGCAGCTCGG CGTGGCCGAG TCGGAGAGCA GCGGGGCCCC CATCGATTTG
    CTCTACGAAC TGATGAAGCA GAAGGGCAAA CCCGATGGGC ACCCCATGGC TCCCCCCACC
    CTCAGCAAAA AATCCGCTTC TCCCAAGGAC GGCATTGCCG GCTCCCCGCG GCCCGCGCTG
    CTCGCGCTCG GCAAAGGCGG TGAGCGCCCG CTGGATGGCC CCATCAACAA AGCCATCAAA
    TCCCCGCCAG GATTCTCCAA GAGCCTCTCG CAGCCCGGAT CCCCCATCCT CAAGAAGGTG
    CCCCCCGCCC TCTCCGGGTC CCCCTCTCCG AAAAACCCCG AGGAGAAGAG CTCGAAGCTA
    TCGCTGAGCC CTCTGCAGAG CTCCCCAAAG GCGCAGTGGC CGCAGGCGGA TGAGGAAGGA
    CCTCTCAATT TGACCGCGGG GGGAGAGCCG GTGCGGGACA TCCGCTGCGA GTTCTGCGGA
    GAGTTCTTTG AGAACCGCAA GGGGCTGTCG AGCCACGCGC GCTCCCACCT CCGACAGATG
    GGGGTGACGG AGTGGTACGT CAACGGCTCA CCCATCGACA CGCTGCGCGA GATCCTCAAG
    CGCCGAACCC ACCCGCGGAG CGGAACCTCC AACCCCATGG GCCCCGGGCA CAAAGCCATG
    GCCAAGACCC TCCTGGCAGC CATGGGACCC CTGGAGCCGC GGGGTCCCGG GGAGCTCCAC
    ATCCCGACTC TGCCCAAGAA GGTCCAGCAG CCTGGCAATC CCCTGGGGCA GTCTCCTACC
    TCGTCCCCAC CTCCCACTGC CCGGAAGATG TTTCCAGGCC TCTCTCCTCC CTCCTTGCAG
    AAGAAGCTCA AACATGACCA GATGAGAGTG GAAATCAAGC GTGAGATGAT GACGGGGGGT
    CTCCACGGAG AGCCCCACCC CTCCGACCAG GCTTGGCCAC CGCTGGAGGA GATGTCACCC
    CTGAACCTCT CCTCCCGGGC CGACCCGGTG CGGGACATCC GCTGCGAGTT CTGCGGAGAG
    TTCTTTGAGA ACCGCAAGGG GCTGTCGAGC CACGCGCGCT CCCACCTCCG ACAGATGGGG
    GTGACGGAGT GGTCGGTGAA CGGCTCTCCC ATCGACACGC TGCGCGAGAT CCTCAAGAAG
    AAATCGAAGC CCTGCGTCAT CAAGAAGGAG CCCCACACCT CCATCATTGA AACCCCCAAA
    GGGTTTGGGG AGGAGGGGAT GGACCCCAAG CCCCCCGGAA AAGTGCTGCA GGCCATGGCT
    CTCCCTCCGC TGGGCGGGCG GACGGGGAAA CCCGGTGGCT CCAGCATGGG CCGCGAGCTC
    TCGCTGTCGC CGCTCACCAC CAAGCCCCAC GGTGCCTTCT TGACACCACT GGCCACCAAA
    CGGCCACTGC AGGACGACCG CCTGGGTCCC CACCCCGAGG TGAAGCACAA GGCCTACATC
    CAGACCGAGC TGCCCTTCAA AACCAAGTCC GTACACGACA AGCCCACGCA CACATCCACC
    GAAGCGTGCT GCGAGCTCTG CGGCCTCTAC TTCGAGAACC GCAAGGCTCT GGCCAGCCAC
    GCGCGGGCGC ACCTCCGGCA ATTCGGCGTC ACCGAATGGT GCGTCAACGG CTCTCCCATC
    GAGACGCTCA GCGAATGGAT CCGGCACCGG CCGCAGAAAG CCGGCGCGTA CCGCAGCTAC
    ATCCAGGGGG GCCGACCCTT CACAAAGAAG TTCAGGAATT CTTCGCACGC CCGAGACCAC
    GACGGAGCGC GGAGGATGCC GCTGAGCCTG CAGCACGGCG GCATGGCCCT GCTGAGCAAA
    GGGCTGGCGG CGGAGCTGGC GCACGGGGAG CCCTGCAAGA TCCTGGACGG CACCGGGGAG
    CGGCCCATGG TCACCTCGCC CCTCTCGCTG GTGAAGATGG AAGAACATCA GCGCACGAAT
    TTCCACAAGT TCGAGCGGAG GCAGGCAAGG CCCCTGGATC CCACCCTGCA CAGGGAAGAG
    GAGGGGGCCG ACTTCCAGCA GAAGATGGAG GAGACCCGCC AGCCCCCCCC ANGGATGAGG
    CCGGTGCCCT CGCTGGTCCC CCGCCCGCCA CAGACCTCCC TGGTGAAGTT TGTGGGTAAC
    ATCTACACCC TCAAGTGCAG GTTCTGTGAG GTGGAGTTCC AGGGCCCTCT CTCCATCCAG
    GAGGAGTGGG TGCGACACCT CCAGCGACAC ATCCTGGAAA TGAATTTCTC CAAAGCAGAC
    CCCCTGCGGG GGGACGCCCC GGCCCCCGAA CCCCCCGCCG TGGCCGAGGC TCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015158171.1
    CDS297..5933
    Translation

    Target ORF information:

    RefSeq Version XM_015302685.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus RAS protein activator like 3 (RASAL3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015302685.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGGAGAAAG CTGTCCCTAC TTTATCAGAG CAACAGCGAG AGCGCCAGGG AGATGGAAGC 
    AGAGCCCTGC TAATGCCACT GGAGCCAAAC AAAGAGAATT TTGCTGCCCC TGATTGCCCC
    CAAGGTGAAG AGAGAAGAGC TGCTTTTGTG AAAGATGCAA AAAGGCCTCT GGAGAGCGCG
    GCTCTATCTG GGACATCGGT GGAAGTGGGG ACGGAGCTCG ACGAAGCCGC CTTCTACACC
    GCTGATGACT TGGTGACAAT GAGCAGGGAC ACAAAGGGAC CCTCCGGCAC CGATCTCTCC
    CCCCTACAAG AGGACTCCGT CTTCTTCTCT CCGCTGTCCC GCCAAGAGCC CGACATTGCC
    CTGGAAGCCT GCGACGTGGC CGATGAGCCT GATCCTGCCC TCTCCGGTGC TGTCCCAGCC
    CCAGCTCACA GGACCGAACT CGGCTCCAAG ACCGAAGAGC ATTTAGAGTT CCAGAAGGCT
    TTGTACAGAG CGGACGCCAG GAGCTCCCTG GAGCTGCGCG CCATGACCGT GTCCGGCAGC
    GTGCCCTGGG ACACCTCCGC CCTGGAGACC CGAGCGGGTG ATGGCAGAGA AGAAAGCACT
    TTAGAGAGAC TCTCTAGCTT GAAGGACTCA AAGGACGTGT CGGCGTTCCC GGGGGATCCC
    CAGGTTGTAA ATACGGTGCG GGTGGATCCT GAGGACCTGG AGAGCGACGC TGAGGAGCTG
    GGCGCCGGCA TGGCGGCAGC TGGGACGCCC AACCCTTGCA CGGGGCCACC GGTGGGTAGT
    GGGGAGGAGA AATACCTTAA ACCTGAACCA AAGCAACTTG AAGGCGTCGG CAAAGAGCAC
    TTAGAGAAAA GCAGGAGGGG GATCCAGAGG GTCGACTGGA TTTCTCGACC CAACAGAAGC
    CCGAGTCCTC ACTACAAAGA CGTGAGCAAG CCGACGGATG TAGCAACCAA CGTACCCTTC
    CGGGGACAGG CTGTGCGTGA AGTGCCTCCT CCGCTGACTC CGGCAGTGTT CAGGAAATCT
    CTGAACCCTG TCCTCAGCAA GTCCAAAACC CTCGAGACCC CCTCGTCCCA CAGGAAGGGG
    CTGGGGGTCG ACTCGGACGC GGGTGAGATG GGCGCGCTCA GCGCAGCTGA GTGGACCATC
    CCAAAGCCGG CCCTGCAGCT CAGCACCGAC CTCGCCATCG GCAAGCAGTC GTGGACGGTG
    AATTCGGAGG ATTCGGTGGA GAGGATCCCG TTGATGTCCC TGGAGCCCAC TCCCTGCAGC
    TCCTATGACG TGGAGATGAG GCCCTACGTG TGCAGCGTGC TGGTGGAGGA GCAGGGCAAA
    GAGGAGCTGG GCCCCGAGGA GGACCCCACG GTGTACACGT GCATCGAGTG CAGCATCTAC
    TTCAAGAAGA AGGAGCACCT GATGGATCAC ATGCTCCAGC ACAACCGTGG ACCAGGGAGG
    GACCAAGATG GGGACGCTCT CGGAGGGCAG TGCCAGTTCT GCTGCAGTGA GTGCGGGTGG
    GCGTTTGGGG ACCTTGCATC CCTAGAGCAG CACAAAAGGC TCCACCAGGA ATCCAGGGAA
    AAAATCATTG AGGAGATCCA GAAGCTGAAC GAGTTCCCTG ACGAGGGTCG GGAAGCCCGG
    CTGCAGTGCC CCAAGTGCAT CTTTGGCACC AACTCCTCCA AGATCTTCGT GCAGCACGCC
    AAGATGCATG TCAAGGAGAG GAAGGACCAA GGGGCGAAGA GCTTGAATCT CTACGGGGGC
    GGAGAGCTGC AGGATAGCCC CGTGCACGGT ATGTACAAAC ACTTCAAACC CAATGAGCAC
    ATGCCCCTGC AAGTGCAGGC GCCTCCGCAC GGCAAAGGGC TCAGCACCTG CATGCTCTGC
    AGCTTCCCGG CGCCCAACGA GAACATCCTC AAAGAGCACA TGAAATACGC CCATTCCCAC
    CTCTCTTGGG ACGCGGAGGT GTACGAAGAA GATCCCAACC AACCGGGAAC CAGCAGGGAC
    GCCTACAGCC CCGCCAGGCC GGGCCGCTTT GCAGAGACTG ATTATTTTGG CAAAGCAGAC
    CAGCTCTTCC CTCCACCCCA CCGGGAAAGC GCGTCGCATT ACGACATCCA CAGCTTCACC
    GTAGATCACC CCAGACTCAA CAGAAGCAAT GGGGGTAGCA AAAAGGACTA CTCGACGTCG
    GGGTTCCATG CCAGGAAAGC GGCCCCGTAC GCTGCCCGGC ACAAAAACCT GGGGCTCTCC
    TCCACGAAGG CTTATTCACA GTTTGCTCTG CAGCACCTGA AAAAAAAAAC AGCAGCTCAG
    CACCTCGAAG CCGAAGGCGA CGGTCTGAGG GGTCACCCAA CGGGCCTGGA GGAGGTCGGG
    CACAAATGGA TGTTTATCAA CGAGGTGGGG GGCGTGGAAG GGGAGTTCTC CATGACCACG
    GACATGGACT TGATGGAGCA CAGAGTCACC AAACCCGTCA CCATCCCTCA AGCTGCTTTG
    GACCTCAAGA GGACGTACAG AGACACGTTG AGAGCCATGG ACCCTTTGGT CGCCTCCGAA
    GAGCAGCAGC AGCAGCTGCG GATGATGGTG CCCATCGTTC TGCTGGAGGA GGTCAACCCA
    CACCCCAAAG CAAAGAAGCG GCCCCGGGCA AAGCCCTGCA AGAAGAAAGC AGCATTCCCA
    TCGCGGGAGT TCATGATGGA AGAGCCTCTT CCCTTGGATG TGCTCCTGTT GGACTCTCCT
    TTAGAGTTTG ATGACTTTTT GGACTCCGAC TCGCCCATGT TGAAGAACGA GGAGAGGAAG
    TGCCCGTATT GCCCGGATCG GTTCCACAAC GGCATTGGGT TGGCGAACCA CGTGCGGGGC
    CACCTCAATC GGGTTGGTGT GAGCTACAAC GTCCGGCATT TCATCTCGGC AGAAGAAGTG
    AAAGCTATTG AGCAAAAATT TTCCTTCCAA AAGAAGAAGA AAAAAGTTGC AAACTTCGAC
    CCGAGCACGT TCAGCCTGAT GCGATGCGAG TTCTGCGGGG CCGGCTTCGA CACACGCGCC
    GGGCTCTCCA GCCACGCCAG GGCCCACCTG AGAGACTTTG GGATCACCAA CTGGGAGCTC
    ACCATCTCGC CCATCAACAT CCTCAAGGAG CTGTTGGCCA ACTCGGCGGA GTACCCCATG
    CTGCAGGCGG CGGTGGGAGC GGAGCCCTCA TCCCCGGGCC GAGAGCCGCA CGGCTTCGTG
    CCCCGCAAGA GCATGACCCC CATCTCCGAG TGCAGCATTC CACGCTCTCC TCTGTCCCCA
    TTCCCTCCAT CTTGGGGAGA TGAGTCCTTG CAGTCCTACA GAGACGCCCA GAACCTGACG
    ACGTGCGAGG TGTGCGGCGC CTGCTTCGAA ACCCGCAAAG GGCTCTCCAG CCACGCGCGC
    TCCCACCTGC GGCAGCTCGG CGTGGCCGAG TCGGAGAGCA GCGGGGCCCC CATCGATTTG
    CTCTACGAAC TGATGAAGCA GAAGGGCAAA CCCGATGGGC ACCCCATGGC TCCCCCCACC
    CTCAGCAAAA AATCCGCTTC TCCCAAGGAC GGCATTGCCG GCTCCCCGCG GCCCGCGCTG
    CTCGCGCTCG GCAAAGGCGG TGAGCGCCCG CTGGATGGCC CCATCAACAA AGCCATCAAA
    TCCCCGCCAG GATTCTCCAA GAGCCTCTCG CAGCCCGGAT CCCCCATCCT CAAGAAGGTG
    CCCCCCGCCC TCTCCGGGTC CCCCTCTCCG AAAAACCCCG AGGAGAAGAG CTCGAAGCTA
    TCGCTGAGCC CTCTGCAGAG CTCCCCAAAG GCGCAGTGGC CGCAGGCGGA TGAGGAAGGA
    CCTCTCAATT TGACCGCGGG GGGAGAGCCG GTGCGGGACA TCCGCTGCGA GTTCTGCGGA
    GAGTTCTTTG AGAACCGCAA GGGGCTGTCG AGCCACGCGC GCTCCCACCT CCGACAGATG
    GGGGTGACGG AGTGGTACGT CAACGGCTCA CCCATCGACA CGCTGCGCGA GATCCTCAAG
    CGCCGAACCC ACCCGCGGAG CGGAACCTCC AACCCCATGG GCCCCGGGCA CAAAGCCATG
    GCCAAGACCC TCCTGGCAGC CATGGGACCC CTGGAGCCGC GGGGTCCCGG GGAGCTCCAC
    ATCCCGACTC TGCCCAAGAA GGTCCAGCAG CCTGGCAATC CCCTGGGGCA GTCTCCTACC
    TCGTCCCCAC CTCCCACTGC CCGGAAGATG TTTCCAGGCC TCTCTCCTCC CTCCTTGCAG
    AAGAAGCTCA AACATGACCA GATGAGAGTG GAAATCAAGC GTGAGATGAT GACGGGGGGT
    CTCCACGGAG AGCCCCACCC CTCCGACCAG GCTTGGCCAC CGCTGGAGGA GATGTCACCC
    CTGAACCTCT CCTCCCGGGC CGACCCGGTG CGGGACATCC GCTGCGAGTT CTGCGGAGAG
    TTCTTTGAGA ACCGCAAGGG GCTGTCGAGC CACGCGCGCT CCCACCTCCG ACAGATGGGG
    GTGACGGAGT GGTCGGTGAA CGGCTCTCCC ATCGACACGC TGCGCGAGAT CCTCAAGAAG
    AAATCGAAGC CCTGCGTCAT CAAGAAGGAG CCCCACACCT CCATCATTGA AACCCCCAAA
    GGGTTTGGGG AGGAGGGGAT GGACCCCAAG CCCCCCGGAA AAGTGCTGCA GGCCATGGCT
    CTCCCTCCGC TGGGCGGGCG GACGGGGAAA CCCGGTGGCT CCAGCATGGG CCGCGAGCTC
    TCGCTGTCGC CGCTCACCAC CAAGCCCCAC GGTGCCTTCT TGACACCACT GGCCACCAAA
    CGGCCACTGC AGGACGACCG CCTGGGTCCC CACCCCGAGG TGAAGCACAA GGCCTACATC
    CAGACCGAGC TGCCCTTCAA AACCAAGTCC GTACACGACA AGCCCACGCA CACATCCACC
    GAAGCGTGCT GCGAGCTCTG CGGCCTCTAC TTCGAGAACC GCAAGGCTCT GGCCAGCCAC
    GCGCGGGCGC ACCTCCGGCA ATTCGGCGTC ACCGAATGGT GCGTCAACGG CTCTCCCATC
    GAGACGCTCA GCGAATGGAT CCGGCACCGG CCGCAGAAAG CCGGCGCGTA CCGCAGCTAC
    ATCCAGGGGG GCCGACCCTT CACAAAGAAG TTCAGGAATT CTTCGCACGC CCGAGACCAC
    GACGGAGCGC GGAGGATGCC GCTGAGCCTG CAGCACGGCG GCATGGCCCT GCTGAGCAAA
    GGGCTGGCGG CGGAGCTGGC GCACGGGGAG CCCTGCAAGA TCCTGGACGG CACCGGGGAG
    CGGCCCATGG TCACCTCGCC CCTCTCGCTG GTGAAGATGG AAGAACATCA GCGCACGAAT
    TTCCACAAGT TCGAGCGGAG GCAGGCAAGG CCCCTGGATC CCACCCTGCA CAGGGAAGAG
    GAGGGGGCCG ACTTCCAGCA GAAGATGGAG GAGACCCGCC AGCCCCCCCC ANGGATGAGG
    CCGGTGCCCT CGCTGGTCCC CCGCCCGCCA CAGACCTCCC TGGTGAAGTT TGTGGGTAAC
    ATCTACACCC TCAAGTGCAG GTTCTGTGAG GTGGAGTTCC AGGGCCCTCT CTCCATCCAG
    GAGGAGTGGG TGCGACACCT CCAGCGACAC ATCCTGGAAA TGAATTTCTC CAAAGCAGAC
    CCCCTGCGGG GGGACGCCCC GGCCCCCGAA CCCCCCGCCG TGGCCGAGGC TCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.