RASAL3 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_025145733.1	XP_025001501.1	protein Wiz isoform X7
XM_025145736.1	XP_025001504.1	protein Wiz isoform X8
XM_015302685.2	XP_015158171.2	protein Wiz isoform X5
XM_025145737.1	XP_025001505.1	protein Wiz isoform X9
XM_015302685.1	XP_015158171.1	LOW QUALITY PROTEIN: protein Wiz
XM_025145727.1	XP_025001495.1	protein Wiz isoform X1
XM_025145728.1	XP_025001496.1	protein Wiz isoform X2
XM_025145731.1	XP_025001499.1	protein Wiz isoform X6
XM_025145735.1	XP_025001503.1	protein Wiz isoform X7
XM_025145729.1	XP_025001497.1	protein Wiz isoform X3
XM_025145730.1	XP_025001498.1	protein Wiz isoform X4
XM_025145732.1	XP_025001500.1	protein Wiz isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa149226	XM_025145736.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa149227	XM_025145737.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa149224	XM_025145731.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa149225	XM_025145732.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa149225	XM_025145733.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa149225	XM_025145735.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OGa149219	XM_025145727.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa149220	XM_025145728.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa149221	XM_025145729.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa149222	XM_025145730.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa149223	XM_015302685.2 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OGa49766	XM_015302685.1 Latest version!	Gallus gallus RAS protein activator like 3 (RASAL3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

RASAL3 ( XM_025145736.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001504.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OGa149226
Clone ID Related Accession (Same CDS sequence)	XM_025145736.1
Accession Version	XM_025145736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGGT 
GAGCGGCCCC TCCCCCCGGC CCCGCCGCCT TCTTCTCCCC GTCGCTCTCG GCCCCACCGC 
GGGGCCCGGC CCGGGGCTGA GGGAGCGCGG GGCCATTCCC GGCGCCGCGC CGCGCTGTGG 
GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA 
CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC 
ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA 
ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001504.1
CDS	39..2810
Translation	MAASTSSPPKVTKAAAVPRPRDRDRGGDGEPGPEATPEPGERPLPPAPPPSSPRRSRPHRGARPGAEGARGHSRRRAALWVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145736.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145736.1

ORF Insert Sequence:

ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGGT 
GAGCGGCCCC TCCCCCCGGC CCCGCCGCCT TCTTCTCCCC GTCGCTCTCG GCCCCACCGC 
GGGGCCCGGC CCGGGGCTGA GGGAGCGCGG GGCCATTCCC GGCGCCGCGC CGCGCTGTGG 
GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA 
CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC 
ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA 
ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145737.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001505.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OGa149227
Clone ID Related Accession (Same CDS sequence)	XM_025145737.1
Accession Version	XM_025145737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGTT 
CTGGCCCCGG AGGAGGAGGA GGTGGTGGCC ATGGAGGTGG GTTCCCCCCC GCCTCCACCA 
CCGCCACCGC TGCCCAAGAA GAGCTCAGCC CCGGGGCAGT CGGAGCAGCC CGCCCGCATA 
GGGACCAAGC TGTGCGCCGA GCCGCCCGGC GGCAAAGCGG ATCCACACGA CCCCAAAACC 
CAGAACCTGA CGACGTGCGA GGTGTGCGGC GCCTGCTTCG AAACCCGCAA AGGGCTCTCC 
AGCCACGCGC GCTCCCACCT GCGGCAGCTC GGCGTGGCCG AGTCGGAGAG CAGCGGGGCC 
CCCATCGATT TGCTCTACGA ACTGATGAAG CAGAAGGGCA AACCCGATGG GCACCCCATG 
GCTCCCCCCA CCCTCAGCAA AAAATCCGCT TCTCCCAAGG ACGGCATTGC CGGCTCCCCG 
CGGCCCGCGC TGCTCGCGCT CGGCAAAGGC GGTGAGCGCC CGCTGGATGG CCCCATCAAC 
AAAGCCATCA AATCCCCGCC AGGATTCTCC AAGAGCCTCT CGCAGCCCGG ATCCCCCATC 
CTCAAGAAGG TGCCCCCCGC CCTCTCCGGG TCCCCCTCTC CGAAAAACCC CGAGGAGAAG 
AGCTCGAAGC TATCGCTGAG CCCTCTGCAG AGCTCCCCAA AGGCGCAGTG GCCGCAGGCG 
GATGAGGAAG GACCTCTCAA TTTGACCGCG GGGGGAGAGC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTAC GTCAACGGCT CACCCATCGA CACGCTGCGC 
GAGATCCTCA AGCGCCGAAC CCACCCGCGG AGCGGAACCT CCAACCCCAT GGGCCCCGGG 
CACAAAGCCA TGGCCAAGAC CCTCCTGGCA GCCATGGGAC CCCTGGAGCC GCGGGGTCCC 
GGGGAGCTCC ACATCCCGAC TCTGCCCAAG AAGGTCCAGC AGCCTGGCAA TCCCCTGGGG 
CAGTCTCCTA CCTCGTCCCC ACCTCCCACT GCCCGGAAGA TGTTTCCAGG CCTCTCTCCT 
CCCTCCTTGC AGAAGAAGCT CAAACATGAC CAGATGAGAG TGGAAATCAA GCGTGAGATG 
ATGACGGGGG GTCTCCACGG AGAGCCCCAC CCCTCCGACC AGGCTTGGCC ACCGCTGGAG 
GAGATGTCAC CCCTGAACCT CTCCTCCCGG GCCGACCCGG TGCGGGACAT CCGCTGCGAG 
TTCTGCGGAG AGTTCTTTGA GAACCGCAAG GGGCTGTCGA GCCACGCGCG CTCCCACCTC 
CGACAGATGG GGGTGACGGA GTGGTCGGTG AACGGCTCTC CCATCGACAC GCTGCGCGAG 
ATCCTCAAGA AGAAATCGAA GCCCTGCGTC ATCAAGAAGG AGCCCCACAC CTCCATCATT 
GAAACCCCCA AAGGGTTTGG GGAGGAGGGG ATGGACCCCA AGCCCCCCGG AAAAGTGCTG 
CAGGCCATGG CTCTCCCTCC GCTGGGCGGG CGGACGGGGA AACCCGGTGG CTCCAGCATG 
GGCCGCGAGC TCTCGCTGTC GCCGCTCACC ACCAAGCCCC ACGGTGCCTT CTTGACACCA 
CTGGCCACCA AACGGCCACT GCAGGACGAC CGCCTGGGTC CCCACCCCGA GGTGAAGCAC 
AAGGCCTACA TCCAGACCGA GCTGCCCTTC AAAACCAAGT CCGTACACGA CAAGCCCACG 
CACACATCCA CCGAAGCGTG CTGCGAGCTC TGCGGCCTCT ACTTCGAGAA CCGCAAGGCT 
CTGGCCAGCC ACGCGCGGGC GCACCTCCGG CAATTCGGCG TCACCGAATG GTGCGTCAAC 
GGCTCTCCCA TCGAGACGCT CAGCGAATGG ATCCGGCACC GGCCGCAGAA AGCCGGCGCG 
TACCGCAGCT ACATCCAGGG GGGCCGACCC TTCACAAAGA AGTTCAGGAA TTCTTCGCAC 
GCCCGAGACC ACGACGGAGC GCGGAGGATG CCGCTGAGCC TGCAGCACGG CGGCATGGCC 
CTGCTGAGCA AAGGGCTGGC GGCGGAGCTG GCGCACGGGG AGCCCTGCAA GATCCTGGAC 
GGCACCGGGG AGCGGCCCAT GGTCACCTCG CCCCTCTCGC TGGTGAAGAT GGAAGAACAT 
CAGCGCACGA ATTTCCACAA GTTCGAGCGG AGGCAGGCAA GGCCCCTGGA TCCCACCCTG 
CACAGGGAAG AGGAGGGGGC CGACTTCCAG CAGAAGATGG AGGAGACCCG CCAGCCCCCC 
CCCAGGATGA GGCCGGTGCC CTCGCTGGTC CCCCGCCCGC CACAGACCTC CCTGGTGAAG 
TTTGTGGGTA ACATCTACAC CCTCAAGTGC AGGTTCTGTG AGGTGGAGTT CCAGGGCCCT 
CTCTCCATCC AGGAGGAGTG GGTGCGACAC CTCCAGCGAC ACATCCTGGA AATGAATTTC 
TCCAAAGCAG ACCCCCTGCG GGGGGACGCC CCGGCCCCCG AACCCCCCGC CGTGGCCGAG 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001505.1
CDS	39..2687
Translation	MAASTSSPPKVTKAAAVPRPRDRDRGGDGEPGPEATPEPVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145737.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145737.1

ORF Insert Sequence:

ATGGCGGCCT CCACCTCCTC GCCGCCCAAA GTGACAAAAG CGGCGGCGGT CCCGCGGCCT 
CGAGACCGGG ACCGGGGCGG GGACGGCGAG CCCGGGCCCG AGGCGACCCC CGAGCCCGTT 
CTGGCCCCGG AGGAGGAGGA GGTGGTGGCC ATGGAGGTGG GTTCCCCCCC GCCTCCACCA 
CCGCCACCGC TGCCCAAGAA GAGCTCAGCC CCGGGGCAGT CGGAGCAGCC CGCCCGCATA 
GGGACCAAGC TGTGCGCCGA GCCGCCCGGC GGCAAAGCGG ATCCACACGA CCCCAAAACC 
CAGAACCTGA CGACGTGCGA GGTGTGCGGC GCCTGCTTCG AAACCCGCAA AGGGCTCTCC 
AGCCACGCGC GCTCCCACCT GCGGCAGCTC GGCGTGGCCG AGTCGGAGAG CAGCGGGGCC 
CCCATCGATT TGCTCTACGA ACTGATGAAG CAGAAGGGCA AACCCGATGG GCACCCCATG 
GCTCCCCCCA CCCTCAGCAA AAAATCCGCT TCTCCCAAGG ACGGCATTGC CGGCTCCCCG 
CGGCCCGCGC TGCTCGCGCT CGGCAAAGGC GGTGAGCGCC CGCTGGATGG CCCCATCAAC 
AAAGCCATCA AATCCCCGCC AGGATTCTCC AAGAGCCTCT CGCAGCCCGG ATCCCCCATC 
CTCAAGAAGG TGCCCCCCGC CCTCTCCGGG TCCCCCTCTC CGAAAAACCC CGAGGAGAAG 
AGCTCGAAGC TATCGCTGAG CCCTCTGCAG AGCTCCCCAA AGGCGCAGTG GCCGCAGGCG 
GATGAGGAAG GACCTCTCAA TTTGACCGCG GGGGGAGAGC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTAC GTCAACGGCT CACCCATCGA CACGCTGCGC 
GAGATCCTCA AGCGCCGAAC CCACCCGCGG AGCGGAACCT CCAACCCCAT GGGCCCCGGG 
CACAAAGCCA TGGCCAAGAC CCTCCTGGCA GCCATGGGAC CCCTGGAGCC GCGGGGTCCC 
GGGGAGCTCC ACATCCCGAC TCTGCCCAAG AAGGTCCAGC AGCCTGGCAA TCCCCTGGGG 
CAGTCTCCTA CCTCGTCCCC ACCTCCCACT GCCCGGAAGA TGTTTCCAGG CCTCTCTCCT 
CCCTCCTTGC AGAAGAAGCT CAAACATGAC CAGATGAGAG TGGAAATCAA GCGTGAGATG 
ATGACGGGGG GTCTCCACGG AGAGCCCCAC CCCTCCGACC AGGCTTGGCC ACCGCTGGAG 
GAGATGTCAC CCCTGAACCT CTCCTCCCGG GCCGACCCGG TGCGGGACAT CCGCTGCGAG 
TTCTGCGGAG AGTTCTTTGA GAACCGCAAG GGGCTGTCGA GCCACGCGCG CTCCCACCTC 
CGACAGATGG GGGTGACGGA GTGGTCGGTG AACGGCTCTC CCATCGACAC GCTGCGCGAG 
ATCCTCAAGA AGAAATCGAA GCCCTGCGTC ATCAAGAAGG AGCCCCACAC CTCCATCATT 
GAAACCCCCA AAGGGTTTGG GGAGGAGGGG ATGGACCCCA AGCCCCCCGG AAAAGTGCTG 
CAGGCCATGG CTCTCCCTCC GCTGGGCGGG CGGACGGGGA AACCCGGTGG CTCCAGCATG 
GGCCGCGAGC TCTCGCTGTC GCCGCTCACC ACCAAGCCCC ACGGTGCCTT CTTGACACCA 
CTGGCCACCA AACGGCCACT GCAGGACGAC CGCCTGGGTC CCCACCCCGA GGTGAAGCAC 
AAGGCCTACA TCCAGACCGA GCTGCCCTTC AAAACCAAGT CCGTACACGA CAAGCCCACG 
CACACATCCA CCGAAGCGTG CTGCGAGCTC TGCGGCCTCT ACTTCGAGAA CCGCAAGGCT 
CTGGCCAGCC ACGCGCGGGC GCACCTCCGG CAATTCGGCG TCACCGAATG GTGCGTCAAC 
GGCTCTCCCA TCGAGACGCT CAGCGAATGG ATCCGGCACC GGCCGCAGAA AGCCGGCGCG 
TACCGCAGCT ACATCCAGGG GGGCCGACCC TTCACAAAGA AGTTCAGGAA TTCTTCGCAC 
GCCCGAGACC ACGACGGAGC GCGGAGGATG CCGCTGAGCC TGCAGCACGG CGGCATGGCC 
CTGCTGAGCA AAGGGCTGGC GGCGGAGCTG GCGCACGGGG AGCCCTGCAA GATCCTGGAC 
GGCACCGGGG AGCGGCCCAT GGTCACCTCG CCCCTCTCGC TGGTGAAGAT GGAAGAACAT 
CAGCGCACGA ATTTCCACAA GTTCGAGCGG AGGCAGGCAA GGCCCCTGGA TCCCACCCTG 
CACAGGGAAG AGGAGGGGGC CGACTTCCAG CAGAAGATGG AGGAGACCCG CCAGCCCCCC 
CCCAGGATGA GGCCGGTGCC CTCGCTGGTC CCCCGCCCGC CACAGACCTC CCTGGTGAAG 
TTTGTGGGTA ACATCTACAC CCTCAAGTGC AGGTTCTGTG AGGTGGAGTT CCAGGGCCCT 
CTCTCCATCC AGGAGGAGTG GGTGCGACAC CTCCAGCGAC ACATCCTGGA AATGAATTTC 
TCCAAAGCAG ACCCCCTGCG GGGGGACGCC CCGGCCCCCG AACCCCCCGC CGTGGCCGAG 
GCTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145731.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001499.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X6, mRNA.

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CloneID	OGa149224
Clone ID Related Accession (Same CDS sequence)	XM_025145731.1
Accession Version	XM_025145731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001499.1
CDS	16..5733
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145731.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145731.1

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145733.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001501.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X8, mRNA.

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CloneID	OGa149225
Clone ID Related Accession (Same CDS sequence)	XM_025145733.1 , XM_025145735.1 , XM_025145732.1
Accession Version	XM_025145733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001501.1
CDS	254..5551
Translation	MTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145733.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145733.1

ORF Insert Sequence:

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145735.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001503.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa149225
Clone ID Related Accession (Same CDS sequence)	XM_025145733.1 , XM_025145735.1 , XM_025145732.1
Accession Version	XM_025145735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001503.1
CDS	224..5521
Translation	MTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145735.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145735.1

ORF Insert Sequence:

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145732.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001500.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X7, mRNA.

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CloneID	OGa149225
Clone ID Related Accession (Same CDS sequence)	XM_025145733.1 , XM_025145735.1 , XM_025145732.1
Accession Version	XM_025145732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001500.1
CDS	8350..13647
Translation	MTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145732.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145732.1

ORF Insert Sequence:

ATGACCGTGT CCGGCAGCGT GCCCTGGGAC ACCTCCGCCC TGGAGACCCG AGCGGGTGAT 
GGCAGAGAAG AAAGCACTTT AGAGAGACTC TCTAGCTTGA AGGACTCAAA GGACGTGTCG 
GCGTTCCCGG GGGATCCCCA GGTTGTAAAT ACGGTGCGGG TGGATCCTGA GGACCTGGAG 
AGCGACGCTG AGGAGCTGGG CGCCGGCATG GCGGCAGCTG GGACGCCCAA CCCTTGCACG 
GGGCCACCGG TGGGTAGTGG GGAGGAGAAA TACCTTAAAC CTGAACCAAA GCAACTTGAA 
GGCGTCGGCA AAGAGCACTT AGAGAAAAGC AGGAGGGGGA TCCAGAGGGT CGACTGGATT 
TCTCGACCCA ACAGAAGCCC GAGTCCTCAC TACAAAGACG TGAGCAAGCC GACGGATGTA 
GCAACCAACG TACCCTTCCG GGGACAGGCT GTGCGTGAAG TGCCTCCTCC GCTGACTCCG 
GCAGTGTTCA GGAAATCTCT GAACCCTGTC CTCAGCAAGT CCAAAACCCT CGAGACCCCC 
TCGTCCCACA GGAAGGGGCT GGGGGTCGAC TCGGACGCGG GTGAGATGGG CGCGCTCAGC 
GCAGCTGAGT GGACCATCCC AAAGCCGGCC CTGCAGCTCA GCACCGACCT CGCCATCGGC 
AAGCAGTCGT GGACGGTGAA TTCGGAGGAT TCGGTGGAGA GGATCCCGTT GATGTCCCTG 
GAGCCCACTC CCTGCAGCTC CTATGACGTG GAGATGAGGC CCTACGTGTG CAGCGTGCTG 
GTGGAGGAGC AGGGCAAAGA GGAGCTGGGC CCCGAGGAGG ACCCCACGGT GTACACGTGC 
ATCGAGTGCA GCATCTACTT CAAGAAGAAG GAGCACCTGA TGGATCACAT GCTCCAGCAC 
AACCGTGGAC CAGGGAGGGA CCAAGATGGG GACGCTCTCG GAGGGCAGTG CCAGTTCTGC 
TGCAGTGAGT GCGGGTGGGC GTTTGGGGAC CTTGCATCCC TAGAGCAGCA CAAAAGGCTC 
CACCAGGAAT CCAGGGAAAA AATCATTGAG GAGATCCAGA AGCTGAACGA GTTCCCTGAC 
GAGGGTCGGG AAGCCCGGCT GCAGTGCCCC AAGTGCATCT TTGGCACCAA CTCCTCCAAG 
ATCTTCGTGC AGCACGCCAA GATGCATGTC AAGGAGAGGA AGGACCAAGG GGCGAAGAGC 
TTGAATCTCT ACGGGGGCGG AGAGCTGCAG GATAGCCCCG TGCACGGTAT GTACAAACAC 
TTCAAACCCA ATGAGCACAT GCCCCTGCAA GTGCAGGCGC CTCCGCACGG CAAAGGGCTC 
AGCACCTGCA TGCTCTGCAG CTTCCCGGCG CCCAACGAGA ACATCCTCAA AGAGCACATG 
AAATACGCCC ATTCCCACCT CTCTTGGGAC GCGGAGGTGT ACGAAGAAGA TCCCAACCAA 
CCGGGAACCA GCAGGGACGC CTACAGCCCC GCCAGGCCGG GCCGCTTTGC AGAGACTGAT 
TATTTTGGCA AAGCAGACCA GCTCTTCCCT CCACCCCACC GGGAAAGCGC GTCGCATTAC 
GACATCCACA GCTTCACCGT AGATCACCCC AGACTCAACA GAAGCAATGG GGGTAGCAAA 
AAGGACTACT CGACGTCGGG GTTCCATGCC AGGAAAGCGG CCCCGTACGC TGCCCGGCAC 
AAAAACCTGG GGCTCTCCTC CACGAAGGCT TATTCACAGT TTGCTCTGCA GCACCTGAAA 
AAAAAAACAG CAGCTCAGCA CCTCGAAGCC GAAGGCGACG GTCTGAGGGG TCACCCAACG 
GGCCTGGAGG AGGTCGGGCA CAAATGGATG TTTATCAACG AGGTGGGGGG CGTGGAAGGG 
GAGTTCTCCA TGACCACGGA CATGGACTTG ATGGAGCACA GAGTCACCAA ACCCGTCACC 
ATCCCTCAAG CTGCTTTGGA CCTCAAGAGG ACGTACAGAG ACACGTTGAG AGCCATGGAC 
CCTTTGGTCG CCTCCGAAGA GCAGCAGCAG CAGCTGCGGA TGATGGTGCC CATCGTTCTG 
CTGGAGGAGG TCAACCCACA CCCCAAAGCA AAGAAGCGGC CCCGGGCAAA GCCCTGCAAG 
AAGAAAGCAG CATTCCCATC GCGGGAGTTC ATGATGGAAG AGCCTCTTCC CTTGGATGTG 
CTCCTGTTGG ACTCTCCTTT AGAGTTTGAT GACTTTTTGG ACTCCGACTC GCCCATGTTG 
AAGAACGAGG AGAGGAAGTG CCCGTATTGC CCGGATCGGT TCCACAACGG CATTGGGTTG 
GCGAACCACG TGCGGGGCCA CCTCAATCGG GTTGGTGTGA GCTACAACGT CCGGCATTTC 
ATCTCGGCAG AAGAAGTGAA AGCTATTGAG CAAAAATTTT CCTTCCAAAA GAAGAAGAAA 
AAAGGTATTG CAAACTTCGA CCCGAGCACG TTCAGCCTGA TGCGATGCGA GTTCTGCGGG 
GCCGGCTTCG ACACACGCGC CGGGCTCTCC AGCCACGCCA GGGCCCACCT GAGAGACTTT 
GGGATCACCA ACTGGGAGCT CACCATCTCG CCCATCAACA TCCTCAAGGA GCTGTTGGCC 
AACTCGGCGG AGTACCCCAT GCTGCAGGCG GCGGTGGGAG CGGAGCCCTC ATCCCCGGGC 
CGAGAGCCGC ACGGCTTCGT GCCCCGCAAG AGCATGACCC CCATCTCCGA GTGCAGCATT 
CCACGCTCTC CTCTGTCCCC ATTCCCTCCA TCTTGGGGAG ATGAGTCCTT GCAGTCCTAC 
AGAGACGTTC TGGCCCCGGA GGAGGAGGAG GTGGTGGCCA TGGAGGTGGG TTCCCCCCCG 
CCTCCACCAC CGCCACCGCT GCCCAAGAAG AGCTCAGCCC CGGGGCAGTC GGAGCAGCCC 
GCCCGCATAG GGACCAAGCT GTGCGCCGAG CCGCCCGGCG GCAAAGCGGA TCCACACGAC 
CCCAAAACCC AGAACCTGAC GACGTGCGAG GTGTGCGGCG CCTGCTTCGA AACCCGCAAA 
GGGCTCTCCA GCCACGCGCG CTCCCACCTG CGGCAGCTCG GCGTGGCCGA GTCGGAGAGC 
AGCGGGGCCC CCATCGATTT GCTCTACGAA CTGATGAAGC AGAAGGGCAA ACCCGATGGG 
CACCCCATGG CTCCCCCCAC CCTCAGCAAA AAATCCGCTT CTCCCAAGGA CGGCATTGCC 
GGCTCCCCGC GGCCCGCGCT GCTCGCGCTC GGCAAAGGCG GTGAGCGCCC GCTGGATGGC 
CCCATCAACA AAGCCATCAA ATCCCCGCCA GGATTCTCCA AGAGCCTCTC GCAGCCCGGA 
TCCCCCATCC TCAAGAAGGT GCCCCCCGCC CTCTCCGGGT CCCCCTCTCC GAAAAACCCC 
GAGGAGAAGA GCTCGAAGCT ATCGCTGAGC CCTCTGCAGA GCTCCCCAAA GGCGCAGTGG 
CCGCAGGCGG ATGAGGAAGG ACCTCTCAAT TTGACCGCGG GGGGAGAGCC GGTGCGGGAC 
ATCCGCTGCG AGTTCTGCGG AGAGTTCTTT GAGAACCGCA AGGGGCTGTC GAGCCACGCG 
CGCTCCCACC TCCGACAGAT GGGGGTGACG GAGTGGTACG TCAACGGCTC ACCCATCGAC 
ACGCTGCGCG AGATCCTCAA GCGCCGAACC CACCCGCGGA GCGGAACCTC CAACCCCATG 
GGCCCCGGGC ACAAAGCCAT GGCCAAGACC CTCCTGGCAG CCATGGGACC CCTGGAGCCG 
CGGGGTCCCG GGGAGCTCCA CATCCCGACT CTGCCCAAGA AGGTCCAGCA GCCTGGCAAT 
CCCCTGGGGC AGTCTCCTAC CTCGTCCCCA CCTCCCACTG CCCGGAAGAT GTTTCCAGGC 
CTCTCTCCTC CCTCCTTGCA GAAGAAGCTC AAACATGACC AGATGAGAGT GGAAATCAAG 
CGTGAGATGA TGACGGGGGG TCTCCACGGA GAGCCCCACC CCTCCGACCA GGCTTGGCCA 
CCGCTGGAGG AGATGTCACC CCTGAACCTC TCCTCCCGGG CCGACCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTCGGTGA ACGGCTCTCC CATCGACACG 
CTGCGCGAGA TCCTCAAGAA GAAATCGAAG CCCTGCGTCA TCAAGAAGGA GCCCCACACC 
TCCATCATTG AAACCCCCAA AGGGTTTGGG GAGGAGGGGA TGGACCCCAA GCCCCCCGGA 
AAAGTGCTGC AGGCCATGGC TCTCCCTCCG CTGGGCGGGC GGACGGGGAA ACCCGGTGGC 
TCCAGCATGG GCCGCGAGCT CTCGCTGTCG CCGCTCACCA CCAAGCCCCA CGGTGCCTTC 
TTGACACCAC TGGCCACCAA ACGGCCACTG CAGGACGACC GCCTGGGTCC CCACCCCGAG 
GTGAAGCACA AGGCCTACAT CCAGACCGAG CTGCCCTTCA AAACCAAGTC CGTACACGAC 
AAGCCCACGC ACACATCCAC CGAAGCGTGC TGCGAGCTCT GCGGCCTCTA CTTCGAGAAC 
CGCAAGGCTC TGGCCAGCCA CGCGCGGGCG CACCTCCGGC AATTCGGCGT CACCGAATGG 
TGCGTCAACG GCTCTCCCAT CGAGACGCTC AGCGAATGGA TCCGGCACCG GCCGCAGAAA 
GCCGGCGCGT ACCGCAGCTA CATCCAGGGG GGCCGACCCT TCACAAAGAA GTTCAGGAAT 
TCTTCGCACG CCCGAGACCA CGACGGAGCG CGGAGGATGC CGCTGAGCCT GCAGCACGGC 
GGCATGGCCC TGCTGAGCAA AGGGCTGGCG GCGGAGCTGG CGCACGGGGA GCCCTGCAAG 
ATCCTGGACG GCACCGGGGA GCGGCCCATG GTCACCTCGC CCCTCTCGCT GGTGAAGATG 
GAAGAACATC AGCGCACGAA TTTCCACAAG TTCGAGCGGA GGCAGGCAAG GCCCCTGGAT 
CCCACCCTGC ACAGGGAAGA GGAGGGGGCC GACTTCCAGC AGAAGATGGA GGAGACCCGC 
CAGCCCCCCC CCAGGATGAG GCCGGTGCCC TCGCTGGTCC CCCGCCCGCC ACAGACCTCC 
CTGGTGAAGT TTGTGGGTAA CATCTACACC CTCAAGTGCA GGTTCTGTGA GGTGGAGTTC 
CAGGGCCCTC TCTCCATCCA GGAGGAGTGG GTGCGACACC TCCAGCGACA CATCCTGGAA 
ATGAATTTCT CCAAAGCAGA CCCCCTGCGG GGGGACGCCC CGGCCCCCGA ACCCCCCGCC 
GTGGCCGAGG CTCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145727.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001495.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X1, mRNA.

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CloneID	OGa149219
Clone ID Related Accession (Same CDS sequence)	XM_025145727.1
Accession Version	XM_025145727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC 
CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC 
TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT 
GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG 
CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG 
CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC 
ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA 
GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC 
AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG 
ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG 
AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG 
CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC 
AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC 
GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC 
GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT 
TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC 
ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC 
CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA 
GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC 
ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG 
CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG 
GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG 
GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG 
AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG 
GCCGAGGCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001495.1
CDS	16..6270
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145727.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145727.1

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC 
CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC 
TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT 
GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG 
CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG 
CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC 
ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA 
GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC 
AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG 
ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG 
AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG 
CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC 
AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC 
GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC 
GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT 
TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC 
ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC 
CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA 
GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC 
ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG 
CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG 
GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG 
GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG 
AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG 
GCCGAGGCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145728.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001496.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X2, mRNA.

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CloneID	OGa149220
Clone ID Related Accession (Same CDS sequence)	XM_025145728.1
Accession Version	XM_025145728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC 
TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC 
ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG 
GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG 
CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC 
TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC 
GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA 
CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC 
ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA 
ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001496.1
CDS	16..6267
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKVANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145728.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145728.1

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC 
TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC 
ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG 
GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG 
CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC 
TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC 
GTTCTGGCCC CGGAGGAGGA GGAGGTGGTG GCCATGGAGG TGGGTTCCCC CCCGCCTCCA 
CCACCGCCAC CGCTGCCCAA GAAGAGCTCA GCCCCGGGGC AGTCGGAGCA GCCCGCCCGC 
ATAGGGACCA AGCTGTGCGC CGAGCCGCCC GGCGGCAAAG CGGATCCACA CGACCCCAAA 
ACCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145729.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001497.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X3, mRNA.

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CloneID	OGa149221
Clone ID Related Accession (Same CDS sequence)	XM_025145729.1
Accession Version	XM_025145729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGA AGAAGCTCAA ACATGACCAG 
ATGAGAGTGG AAATCAAGCG TGAGATGATG ACGGGGGGTC TCCACGGAGA GCCCCACCCC 
TCCGACCAGG CTTGGCCACC GCTGGAGGAG ATGTCACCCC TGAACCTCTC CTCCCGGGCC 
GACCCGGTGC GGGACATCCG CTGCGAGTTC TGCGGAGAGT TCTTTGAGAA CCGCAAGGGG 
CTGTCGAGCC ACGCGCGCTC CCACCTCCGA CAGATGGGGG TGACGGAGTG GTCGGTGAAC 
GGCTCTCCCA TCGACACGCT GCGCGAGATC CTCAAGAAGA AATCGAAGCC CTGCGTCATC 
AAGAAGGAGC CCCACACCTC CATCATTGAA ACCCCCAAAG GGTTTGGGGA GGAGGGGATG 
GACCCCAAGC CCCCCGGAAA AGTGCTGCAG GCCATGGCTC TCCCTCCGCT GGGCGGGCGG 
ACGGGGAAAC CCGGTGGCTC CAGCATGGGC CGCGAGCTCT CGCTGTCGCC GCTCACCACC 
AAGCCCCACG GTGCCTTCTT GACACCACTG GCCACCAAAC GGCCACTGCA GGACGACCGC 
CTGGGTCCCC ACCCCGAGGT GAAGCACAAG GCCTACATCC AGACCGAGCT GCCCTTCAAA 
ACCAAGTCCG TACACGACAA GCCCACGCAC ACATCCACCG AAGCGTGCTG CGAGCTCTGC 
GGCCTCTACT TCGAGAACCG CAAGGCTCTG GCCAGCCACG CGCGGGCGCA CCTCCGGCAA 
TTCGGCGTCA CCGAATGGTG CGTCAACGGC TCTCCCATCG AGACGCTCAG CGAATGGATC 
CGGCACCGGC CGCAGAAAGC CGGCGCGTAC CGCAGCTACA TCCAGGGGGG CCGACCCTTC 
ACAAAGAAGT TCAGGAATTC TTCGCACGCC CGAGACCACG ACGGAGCGCG GAGGATGCCG 
CTGAGCCTGC AGCACGGCGG CATGGCCCTG CTGAGCAAAG GGCTGGCGGC GGAGCTGGCG 
CACGGGGAGC CCTGCAAGAT CCTGGACGGC ACCGGGGAGC GGCCCATGGT CACCTCGCCC 
CTCTCGCTGG TGAAGATGGA AGAACATCAG CGCACGAATT TCCACAAGTT CGAGCGGAGG 
CAGGCAAGGC CCCTGGATCC CACCCTGCAC AGGGAAGAGG AGGGGGCCGA CTTCCAGCAG 
AAGATGGAGG AGACCCGCCA GCCCCCCCCC AGGATGAGGC CGGTGCCCTC GCTGGTCCCC 
CGCCCGCCAC AGACCTCCCT GGTGAAGTTT GTGGGTAACA TCTACACCCT CAAGTGCAGG 
TTCTGTGAGG TGGAGTTCCA GGGCCCTCTC TCCATCCAGG AGGAGTGGGT GCGACACCTC 
CAGCGACACA TCCTGGAAAT GAATTTCTCC AAAGCAGACC CCCTGCGGGG GGACGCCCCG 
GCCCCCGAAC CCCCCGCCGT GGCCGAGGCT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001497.1
CDS	16..6171
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDVLAPEEEEVVAMEVGSPPPPPPPPLPKKSSAPGQSEQPARIGTKLCAEPPGGKADPHDPKTQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145729.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145729.1

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGTTCTGG CCCCGGAGGA GGAGGAGGTG GTGGCCATGG AGGTGGGTTC CCCCCCGCCT 
CCACCACCGC CACCGCTGCC CAAGAAGAGC TCAGCCCCGG GGCAGTCGGA GCAGCCCGCC 
CGCATAGGGA CCAAGCTGTG CGCCGAGCCG CCCGGCGGCA AAGCGGATCC ACACGACCCC 
AAAACCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGA AGAAGCTCAA ACATGACCAG 
ATGAGAGTGG AAATCAAGCG TGAGATGATG ACGGGGGGTC TCCACGGAGA GCCCCACCCC 
TCCGACCAGG CTTGGCCACC GCTGGAGGAG ATGTCACCCC TGAACCTCTC CTCCCGGGCC 
GACCCGGTGC GGGACATCCG CTGCGAGTTC TGCGGAGAGT TCTTTGAGAA CCGCAAGGGG 
CTGTCGAGCC ACGCGCGCTC CCACCTCCGA CAGATGGGGG TGACGGAGTG GTCGGTGAAC 
GGCTCTCCCA TCGACACGCT GCGCGAGATC CTCAAGAAGA AATCGAAGCC CTGCGTCATC 
AAGAAGGAGC CCCACACCTC CATCATTGAA ACCCCCAAAG GGTTTGGGGA GGAGGGGATG 
GACCCCAAGC CCCCCGGAAA AGTGCTGCAG GCCATGGCTC TCCCTCCGCT GGGCGGGCGG 
ACGGGGAAAC CCGGTGGCTC CAGCATGGGC CGCGAGCTCT CGCTGTCGCC GCTCACCACC 
AAGCCCCACG GTGCCTTCTT GACACCACTG GCCACCAAAC GGCCACTGCA GGACGACCGC 
CTGGGTCCCC ACCCCGAGGT GAAGCACAAG GCCTACATCC AGACCGAGCT GCCCTTCAAA 
ACCAAGTCCG TACACGACAA GCCCACGCAC ACATCCACCG AAGCGTGCTG CGAGCTCTGC 
GGCCTCTACT TCGAGAACCG CAAGGCTCTG GCCAGCCACG CGCGGGCGCA CCTCCGGCAA 
TTCGGCGTCA CCGAATGGTG CGTCAACGGC TCTCCCATCG AGACGCTCAG CGAATGGATC 
CGGCACCGGC CGCAGAAAGC CGGCGCGTAC CGCAGCTACA TCCAGGGGGG CCGACCCTTC 
ACAAAGAAGT TCAGGAATTC TTCGCACGCC CGAGACCACG ACGGAGCGCG GAGGATGCCG 
CTGAGCCTGC AGCACGGCGG CATGGCCCTG CTGAGCAAAG GGCTGGCGGC GGAGCTGGCG 
CACGGGGAGC CCTGCAAGAT CCTGGACGGC ACCGGGGAGC GGCCCATGGT CACCTCGCCC 
CTCTCGCTGG TGAAGATGGA AGAACATCAG CGCACGAATT TCCACAAGTT CGAGCGGAGG 
CAGGCAAGGC CCCTGGATCC CACCCTGCAC AGGGAAGAGG AGGGGGCCGA CTTCCAGCAG 
AAGATGGAGG AGACCCGCCA GCCCCCCCCC AGGATGAGGC CGGTGCCCTC GCTGGTCCCC 
CGCCCGCCAC AGACCTCCCT GGTGAAGTTT GTGGGTAACA TCTACACCCT CAAGTGCAGG 
TTCTGTGAGG TGGAGTTCCA GGGCCCTCTC TCCATCCAGG AGGAGTGGGT GCGACACCTC 
CAGCGACACA TCCTGGAAAT GAATTTCTCC AAAGCAGACC CCCTGCGGGG GGACGCCCCG 
GCCCCCGAAC CCCCCGCCGT GGCCGAGGCT CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_025145730.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025001498.1 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X4, mRNA.

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CloneID	OGa149222
Clone ID Related Accession (Same CDS sequence)	XM_025145730.1
Accession Version	XM_025145730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6075bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGCCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC 
CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC 
TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT 
GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG 
CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG 
CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC 
ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA 
GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC 
AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG 
ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG 
AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG 
CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC 
AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC 
GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC 
GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT 
TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC 
ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC 
CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA 
GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC 
ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG 
CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG 
GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG 
GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG 
AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG 
GCCGAGGCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025001498.1
CDS	16..6090
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKGIANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDAQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_025145730.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025145730.1

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GGTATTGCAA ACTTCGACCC GAGCACGTTC AGCCTGATGC GATGCGAGTT CTGCGGGGCC 
GGCTTCGACA CACGCGCCGG GCTCTCCAGC CACGCCAGGG CCCACCTGAG AGACTTTGGG 
ATCACCAACT GGGAGCTCAC CATCTCGCCC ATCAACATCC TCAAGGAGCT GTTGGCCAAC 
TCGGCGGAGT ACCCCATGCT GCAGGCGGCG GTGGGAGCGG AGCCCTCATC CCCGGGCCGA 
GAGCCGCACG GCTTCGTGCC CCGCAAGAGC ATGACCCCCA TCTCCGAGTG CAGCATTCCA 
CGCTCTCCTC TGTCCCCATT CCCTCCATCT TGGGGAGATG AGTCCTTGCA GTCCTACAGA 
GACGCCCAGA ACCTGACGAC GTGCGAGGTG TGCGGCGCCT GCTTCGAAAC CCGCAAAGGG 
CTCTCCAGCC ACGCGCGCTC CCACCTGCGG CAGCTCGGCG TGGCCGAGTC GGAGAGCAGC 
GGGGCCCCCA TCGATTTGCT CTACGAACTG ATGAAGCAGA AGGGCAAACC CGATGGGCAC 
CCCATGGCTC CCCCCACCCT CAGCAAAAAA TCCGCTTCTC CCAAGGACGG CATTGCCGGC 
TCCCCGCGGC CCGCGCTGCT CGCGCTCGGC AAAGGCGGTG AGCGCCCGCT GGATGGCCCC 
ATCAACAAAG CCATCAAATC CCCGCCAGGA TTCTCCAAGA GCCTCTCGCA GCCCGGATCC 
CCCATCCTCA AGAAGGTGCC CCCCGCCCTC TCCGGGTCCC CCTCTCCGAA AAACCCCGAG 
GAGAAGAGCT CGAAGCTATC GCTGAGCCCT CTGCAGAGCT CCCCAAAGGC GCAGTGGCCG 
CAGGCGGATG AGGAAGGACC TCTCAATTTG ACCGCGGGGG GAGAGCCGGT GCGGGACATC 
CGCTGCGAGT TCTGCGGAGA GTTCTTTGAG AACCGCAAGG GGCTGTCGAG CCACGCGCGC 
TCCCACCTCC GACAGATGGG GGTGACGGAG TGGTACGTCA ACGGCTCACC CATCGACACG 
CTGCGCGAGA TCCTCAAGCG CCGAACCCAC CCGCGGAGCG GAACCTCCAA CCCCATGGGC 
CCCGGGCACA AAGCCATGGC CAAGACCCTC CTGGCAGCCA TGGGACCCCT GGAGCCGCGG 
GGTCCCGGGG AGCTCCACAT CCCGACTCTG CCCAAGAAGG TCCAGCAGCC TGGCAATCCC 
CTGGGGCAGT CTCCTACCTC GTCCCCACCT CCCACTGCCC GGAAGATGTT TCCAGGCCTC 
TCTCCTCCCT CCTTGCAGAA GAAGCTCAAA CATGACCAGA TGAGAGTGGA AATCAAGCGT 
GAGATGATGA CGGGGGGTCT CCACGGAGAG CCCCACCCCT CCGACCAGGC TTGGCCACCG 
CTGGAGGAGA TGTCACCCCT GAACCTCTCC TCCCGGGCCG ACCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TCGGTGAACG GCTCTCCCAT CGACACGCTG 
CGCGAGATCC TCAAGAAGAA ATCGAAGCCC TGCGTCATCA AGAAGGAGCC CCACACCTCC 
ATCATTGAAA CCCCCAAAGG GTTTGGGGAG GAGGGGATGG ACCCCAAGCC CCCCGGAAAA 
GTGCTGCAGG CCATGGCTCT CCCTCCGCTG GGCGGGCGGA CGGGGAAACC CGGTGGCTCC 
AGCATGGGCC GCGAGCTCTC GCTGTCGCCG CTCACCACCA AGCCCCACGG TGCCTTCTTG 
ACACCACTGG CCACCAAACG GCCACTGCAG GACGACCGCC TGGGTCCCCA CCCCGAGGTG 
AAGCACAAGG CCTACATCCA GACCGAGCTG CCCTTCAAAA CCAAGTCCGT ACACGACAAG 
CCCACGCACA CATCCACCGA AGCGTGCTGC GAGCTCTGCG GCCTCTACTT CGAGAACCGC 
AAGGCTCTGG CCAGCCACGC GCGGGCGCAC CTCCGGCAAT TCGGCGTCAC CGAATGGTGC 
GTCAACGGCT CTCCCATCGA GACGCTCAGC GAATGGATCC GGCACCGGCC GCAGAAAGCC 
GGCGCGTACC GCAGCTACAT CCAGGGGGGC CGACCCTTCA CAAAGAAGTT CAGGAATTCT 
TCGCACGCCC GAGACCACGA CGGAGCGCGG AGGATGCCGC TGAGCCTGCA GCACGGCGGC 
ATGGCCCTGC TGAGCAAAGG GCTGGCGGCG GAGCTGGCGC ACGGGGAGCC CTGCAAGATC 
CTGGACGGCA CCGGGGAGCG GCCCATGGTC ACCTCGCCCC TCTCGCTGGT GAAGATGGAA 
GAACATCAGC GCACGAATTT CCACAAGTTC GAGCGGAGGC AGGCAAGGCC CCTGGATCCC 
ACCCTGCACA GGGAAGAGGA GGGGGCCGAC TTCCAGCAGA AGATGGAGGA GACCCGCCAG 
CCCCCCCCCA GGATGAGGCC GGTGCCCTCG CTGGTCCCCC GCCCGCCACA GACCTCCCTG 
GTGAAGTTTG TGGGTAACAT CTACACCCTC AAGTGCAGGT TCTGTGAGGT GGAGTTCCAG 
GGCCCTCTCT CCATCCAGGA GGAGTGGGTG CGACACCTCC AGCGACACAT CCTGGAAATG 
AATTTCTCCA AAGCAGACCC CCTGCGGGGG GACGCCCCGG CCCCCGAACC CCCCGCCGTG 
GCCGAGGCTC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_015302685.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158171.2 Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X5, mRNA.

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CloneID	OGa149223
Clone ID Related Accession (Same CDS sequence)	XM_015302685.2
Accession Version	XM_015302685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	protein Wiz isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_028739.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015302685.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC 
TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC 
ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG 
GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG 
CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC 
TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC 
GCCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158171.2
CDS	16..6087
Translation	MAAAGAEAALGLRSPPPRCRRRAGGARRDVVGPRRRGGGGGAARAAQAAAAAAAAEEEEEEEEEEAERGGPDPAAAPGGDLKGPCPLRGESGGGGGAAREARGPGGGGCGAEGGGEGFFRLSLAVNPPESQRGVFFQVTQHNQQGMEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKVANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDAQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPRMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_015302685.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302685.2

ORF Insert Sequence:

ATGGCGGCGG CGGGAGCGGA GGCGGCGCTG GGGCTCCGCT CGCCGCCCCC AAGATGTCGG 
CGTCGGGCGG GCGGCGCGCG GCGTGACGTC GTCGGGCCCC GCCGGCGCGG GGGGGGCGGG 
GGAGCGGCCC GAGCGGCGCA GGCGGCGGCG GCGGCGGCGG CGGCGGAGGA GGAGGAGGAG 
GAGGAGGAGG AAGAGGCGGA GAGGGGGGGC CCGGACCCGG CCGCGGCACC GGGGGGGGAT 
TTAAAGGGAC CATGTCCCCT CCGCGGGGAG AGCGGCGGCG GCGGCGGAGC GGCGAGAGAG 
GCGCGCGGCC CCGGGGGTGG CGGCTGCGGC GCTGAGGGGG GGGGCGAGGG ATTTTTCCGG 
CTGTCCCTGG CCGTCAACCC TCCCGAGTCC CAGCGCGGTG TTTTTTTCCA AGTGACACAG 
CACAATCAGC AAGGGATGGA GAAAGCTGTC CCTACTTTAT CAGAGCAACA GCGAGAGCGC 
CAGGGAGATG GAAGCAGAGC CCTGCTAATG CCACTGGAGC CAAACAAAGA GAATTTTGCT 
GCCCCTGATT GCCCCCAAGG TGAAGAGAGA AGAGCTGCTT TTGTGAAAGA TGCAAAAAGG 
CCTCTGGAGA GCGCGGCTCT ATCTGGGACA TCGGTGGAAG TGGGGACGGA GCTCGACGAA 
GCCGCCTTCT ACACCGCTGA TGACTTGGTG ACAATGAGCA GGGACACAAA GGGACCCTCC 
GGCACCGATC TCTCCCCCCT ACAAGAGGAC TCCGTCTTCT TCTCTCCGCT GTCCCGCCAA 
GAGCCCGACA TTGCCCTGGA AGCCTGCGAC GTGGCCGATG AGCCTGATCC TGCCCTCTCC 
GGTGCTGTCC CAGCCCCAGC TCACAGGACC GAACTCGGCT CCAAGACCGA AGAGCATTTA 
GAGTTCCAGA AGGCTTTGTA CAGAGCGGAC GCCAGGAGCT CCCTGGAGCT GCGCGCCATG 
ACCGTGTCCG GCAGCGTGCC CTGGGACACC TCCGCCCTGG AGACCCGAGC GGGTGATGGC 
AGAGAAGAAA GCACTTTAGA GAGACTCTCT AGCTTGAAGG ACTCAAAGGA CGTGTCGGCG 
TTCCCGGGGG ATCCCCAGGT TGTAAATACG GTGCGGGTGG ATCCTGAGGA CCTGGAGAGC 
GACGCTGAGG AGCTGGGCGC CGGCATGGCG GCAGCTGGGA CGCCCAACCC TTGCACGGGG 
CCACCGGTGG GTAGTGGGGA GGAGAAATAC CTTAAACCTG AACCAAAGCA ACTTGAAGGC 
GTCGGCAAAG AGCACTTAGA GAAAAGCAGG AGGGGGATCC AGAGGGTCGA CTGGATTTCT 
CGACCCAACA GAAGCCCGAG TCCTCACTAC AAAGACGTGA GCAAGCCGAC GGATGTAGCA 
ACCAACGTAC CCTTCCGGGG ACAGGCTGTG CGTGAAGTGC CTCCTCCGCT GACTCCGGCA 
GTGTTCAGGA AATCTCTGAA CCCTGTCCTC AGCAAGTCCA AAACCCTCGA GACCCCCTCG 
TCCCACAGGA AGGGGCTGGG GGTCGACTCG GACGCGGGTG AGATGGGCGC GCTCAGCGCA 
GCTGAGTGGA CCATCCCAAA GCCGGCCCTG CAGCTCAGCA CCGACCTCGC CATCGGCAAG 
CAGTCGTGGA CGGTGAATTC GGAGGATTCG GTGGAGAGGA TCCCGTTGAT GTCCCTGGAG 
CCCACTCCCT GCAGCTCCTA TGACGTGGAG ATGAGGCCCT ACGTGTGCAG CGTGCTGGTG 
GAGGAGCAGG GCAAAGAGGA GCTGGGCCCC GAGGAGGACC CCACGGTGTA CACGTGCATC 
GAGTGCAGCA TCTACTTCAA GAAGAAGGAG CACCTGATGG ATCACATGCT CCAGCACAAC 
CGTGGACCAG GGAGGGACCA AGATGGGGAC GCTCTCGGAG GGCAGTGCCA GTTCTGCTGC 
AGTGAGTGCG GGTGGGCGTT TGGGGACCTT GCATCCCTAG AGCAGCACAA AAGGCTCCAC 
CAGGAATCCA GGGAAAAAAT CATTGAGGAG ATCCAGAAGC TGAACGAGTT CCCTGACGAG 
GGTCGGGAAG CCCGGCTGCA GTGCCCCAAG TGCATCTTTG GCACCAACTC CTCCAAGATC 
TTCGTGCAGC ACGCCAAGAT GCATGTCAAG GAGAGGAAGG ACCAAGGGGC GAAGAGCTTG 
AATCTCTACG GGGGCGGAGA GCTGCAGGAT AGCCCCGTGC ACGGTATGTA CAAACACTTC 
AAACCCAATG AGCACATGCC CCTGCAAGTG CAGGCGCCTC CGCACGGCAA AGGGCTCAGC 
ACCTGCATGC TCTGCAGCTT CCCGGCGCCC AACGAGAACA TCCTCAAAGA GCACATGAAA 
TACGCCCATT CCCACCTCTC TTGGGACGCG GAGGTGTACG AAGAAGATCC CAACCAACCG 
GGAACCAGCA GGGACGCCTA CAGCCCCGCC AGGCCGGGCC GCTTTGCAGA GACTGATTAT 
TTTGGCAAAG CAGACCAGCT CTTCCCTCCA CCCCACCGGG AAAGCGCGTC GCATTACGAC 
ATCCACAGCT TCACCGTAGA TCACCCCAGA CTCAACAGAA GCAATGGGGG TAGCAAAAAG 
GACTACTCGA CGTCGGGGTT CCATGCCAGG AAAGCGGCCC CGTACGCTGC CCGGCACAAA 
AACCTGGGGC TCTCCTCCAC GAAGGCTTAT TCACAGTTTG CTCTGCAGCA CCTGAAAAAA 
AAAACAGCAG CTCAGCACCT CGAAGCCGAA GGCGACGGTC TGAGGGGTCA CCCAACGGGC 
CTGGAGGAGG TCGGGCACAA ATGGATGTTT ATCAACGAGG TGGGGGGCGT GGAAGGGGAG 
TTCTCCATGA CCACGGACAT GGACTTGATG GAGCACAGAG TCACCAAACC CGTCACCATC 
CCTCAAGCTG CTTTGGACCT CAAGAGGACG TACAGAGACA CGTTGAGAGC CATGGACCCT 
TTGGTCGCCT CCGAAGAGCA GCAGCAGCAG CTGCGGATGA TGGTGCCCAT CGTTCTGCTG 
GAGGAGGTCA ACCCACACCC CAAAGCAAAG AAGCGGCCCC GGGCAAAGCC CTGCAAGAAG 
AAAGCAGCAT TCCCATCGCG GGAGTTCATG ATGGAAGAGC CTCTTCCCTT GGATGTGCTC 
CTGTTGGACT CTCCTTTAGA GTTTGATGAC TTTTTGGACT CCGACTCGCC CATGTTGAAG 
AACGAGGAGA GGAAGTGCCC GTATTGCCCG GATCGGTTCC ACAACGGCAT TGGGTTGGCG 
AACCACGTGC GGGGCCACCT CAATCGGGTT GGTGTGAGCT ACAACGTCCG GCATTTCATC 
TCGGCAGAAG AAGTGAAAGC TATTGAGCAA AAATTTTCCT TCCAAAAGAA GAAGAAAAAA 
GTTGCAAACT TCGACCCGAG CACGTTCAGC CTGATGCGAT GCGAGTTCTG CGGGGCCGGC 
TTCGACACAC GCGCCGGGCT CTCCAGCCAC GCCAGGGCCC ACCTGAGAGA CTTTGGGATC 
ACCAACTGGG AGCTCACCAT CTCGCCCATC AACATCCTCA AGGAGCTGTT GGCCAACTCG 
GCGGAGTACC CCATGCTGCA GGCGGCGGTG GGAGCGGAGC CCTCATCCCC GGGCCGAGAG 
CCGCACGGCT TCGTGCCCCG CAAGAGCATG ACCCCCATCT CCGAGTGCAG CATTCCACGC 
TCTCCTCTGT CCCCATTCCC TCCATCTTGG GGAGATGAGT CCTTGCAGTC CTACAGAGAC 
GCCCAGAACC TGACGACGTG CGAGGTGTGC GGCGCCTGCT TCGAAACCCG CAAAGGGCTC 
TCCAGCCACG CGCGCTCCCA CCTGCGGCAG CTCGGCGTGG CCGAGTCGGA GAGCAGCGGG 
GCCCCCATCG ATTTGCTCTA CGAACTGATG AAGCAGAAGG GCAAACCCGA TGGGCACCCC 
ATGGCTCCCC CCACCCTCAG CAAAAAATCC GCTTCTCCCA AGGACGGCAT TGCCGGCTCC 
CCGCGGCCCG CGCTGCTCGC GCTCGGCAAA GGCGGTGAGC GCCCGCTGGA TGGCCCCATC 
AACAAAGCCA TCAAATCCCC GCCAGGATTC TCCAAGAGCC TCTCGCAGCC CGGATCCCCC 
ATCCTCAAGA AGGTGCCCCC CGCCCTCTCC GGGTCCCCCT CTCCGAAAAA CCCCGAGGAG 
AAGAGCTCGA AGCTATCGCT GAGCCCTCTG CAGAGCTCCC CAAAGGCGCA GTGGCCGCAG 
GCGGATGAGG AAGGACCTCT CAATTTGACC GCGGGGGGAG AGCCGGTGCG GGACATCCGC 
TGCGAGTTCT GCGGAGAGTT CTTTGAGAAC CGCAAGGGGC TGTCGAGCCA CGCGCGCTCC 
CACCTCCGAC AGATGGGGGT GACGGAGTGG TACGTCAACG GCTCACCCAT CGACACGCTG 
CGCGAGATCC TCAAGCGCCG AACCCACCCG CGGAGCGGAA CCTCCAACCC CATGGGCCCC 
GGGCACAAAG CCATGGCCAA GACCCTCCTG GCAGCCATGG GACCCCTGGA GCCGCGGGGT 
CCCGGGGAGC TCCACATCCC GACTCTGCCC AAGAAGGTCC AGCAGCCTGG CAATCCCCTG 
GGGCAGTCTC CTACCTCGTC CCCACCTCCC ACTGCCCGGA AGATGTTTCC AGGCCTCTCT 
CCTCCCTCCT TGCAGAAGAA GCTCAAACAT GACCAGATGA GAGTGGAAAT CAAGCGTGAG 
ATGATGACGG GGGGTCTCCA CGGAGAGCCC CACCCCTCCG ACCAGGCTTG GCCACCGCTG 
GAGGAGATGT CACCCCTGAA CCTCTCCTCC CGGGCCGACC CGGTGCGGGA CATCCGCTGC 
GAGTTCTGCG GAGAGTTCTT TGAGAACCGC AAGGGGCTGT CGAGCCACGC GCGCTCCCAC 
CTCCGACAGA TGGGGGTGAC GGAGTGGTCG GTGAACGGCT CTCCCATCGA CACGCTGCGC 
GAGATCCTCA AGAAGAAATC GAAGCCCTGC GTCATCAAGA AGGAGCCCCA CACCTCCATC 
ATTGAAACCC CCAAAGGGTT TGGGGAGGAG GGGATGGACC CCAAGCCCCC CGGAAAAGTG 
CTGCAGGCCA TGGCTCTCCC TCCGCTGGGC GGGCGGACGG GGAAACCCGG TGGCTCCAGC 
ATGGGCCGCG AGCTCTCGCT GTCGCCGCTC ACCACCAAGC CCCACGGTGC CTTCTTGACA 
CCACTGGCCA CCAAACGGCC ACTGCAGGAC GACCGCCTGG GTCCCCACCC CGAGGTGAAG 
CACAAGGCCT ACATCCAGAC CGAGCTGCCC TTCAAAACCA AGTCCGTACA CGACAAGCCC 
ACGCACACAT CCACCGAAGC GTGCTGCGAG CTCTGCGGCC TCTACTTCGA GAACCGCAAG 
GCTCTGGCCA GCCACGCGCG GGCGCACCTC CGGCAATTCG GCGTCACCGA ATGGTGCGTC 
AACGGCTCTC CCATCGAGAC GCTCAGCGAA TGGATCCGGC ACCGGCCGCA GAAAGCCGGC 
GCGTACCGCA GCTACATCCA GGGGGGCCGA CCCTTCACAA AGAAGTTCAG GAATTCTTCG 
CACGCCCGAG ACCACGACGG AGCGCGGAGG ATGCCGCTGA GCCTGCAGCA CGGCGGCATG 
GCCCTGCTGA GCAAAGGGCT GGCGGCGGAG CTGGCGCACG GGGAGCCCTG CAAGATCCTG 
GACGGCACCG GGGAGCGGCC CATGGTCACC TCGCCCCTCT CGCTGGTGAA GATGGAAGAA 
CATCAGCGCA CGAATTTCCA CAAGTTCGAG CGGAGGCAGG CAAGGCCCCT GGATCCCACC 
CTGCACAGGG AAGAGGAGGG GGCCGACTTC CAGCAGAAGA TGGAGGAGAC CCGCCAGCCC 
CCCCCCAGGA TGAGGCCGGT GCCCTCGCTG GTCCCCCGCC CGCCACAGAC CTCCCTGGTG 
AAGTTTGTGG GTAACATCTA CACCCTCAAG TGCAGGTTCT GTGAGGTGGA GTTCCAGGGC 
CCTCTCTCCA TCCAGGAGGA GTGGGTGCGA CACCTCCAGC GACACATCCT GGAAATGAAT 
TTCTCCAAAG CAGACCCCCT GCGGGGGGAC GCCCCGGCCC CCGAACCCCC CGCCGTGGCC 
GAGGCTCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RASAL3 ( XM_015302685.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015158171.1 Gallus gallus RAS protein activator like 3 (RASAL3), mRNA.

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CloneID	OGa49766
Clone ID Related Accession (Same CDS sequence)	XM_015302685.1
Accession Version	XM_015302685.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	LOW QUALITY PROTEIN: protein Wiz
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_463422.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGAGAAAG CTGTCCCTAC TTTATCAGAG CAACAGCGAG AGCGCCAGGG AGATGGAAGC 
AGAGCCCTGC TAATGCCACT GGAGCCAAAC AAAGAGAATT TTGCTGCCCC TGATTGCCCC 
CAAGGTGAAG AGAGAAGAGC TGCTTTTGTG AAAGATGCAA AAAGGCCTCT GGAGAGCGCG 
GCTCTATCTG GGACATCGGT GGAAGTGGGG ACGGAGCTCG ACGAAGCCGC CTTCTACACC 
GCTGATGACT TGGTGACAAT GAGCAGGGAC ACAAAGGGAC CCTCCGGCAC CGATCTCTCC 
CCCCTACAAG AGGACTCCGT CTTCTTCTCT CCGCTGTCCC GCCAAGAGCC CGACATTGCC 
CTGGAAGCCT GCGACGTGGC CGATGAGCCT GATCCTGCCC TCTCCGGTGC TGTCCCAGCC 
CCAGCTCACA GGACCGAACT CGGCTCCAAG ACCGAAGAGC ATTTAGAGTT CCAGAAGGCT 
TTGTACAGAG CGGACGCCAG GAGCTCCCTG GAGCTGCGCG CCATGACCGT GTCCGGCAGC 
GTGCCCTGGG ACACCTCCGC CCTGGAGACC CGAGCGGGTG ATGGCAGAGA AGAAAGCACT 
TTAGAGAGAC TCTCTAGCTT GAAGGACTCA AAGGACGTGT CGGCGTTCCC GGGGGATCCC 
CAGGTTGTAA ATACGGTGCG GGTGGATCCT GAGGACCTGG AGAGCGACGC TGAGGAGCTG 
GGCGCCGGCA TGGCGGCAGC TGGGACGCCC AACCCTTGCA CGGGGCCACC GGTGGGTAGT 
GGGGAGGAGA AATACCTTAA ACCTGAACCA AAGCAACTTG AAGGCGTCGG CAAAGAGCAC 
TTAGAGAAAA GCAGGAGGGG GATCCAGAGG GTCGACTGGA TTTCTCGACC CAACAGAAGC 
CCGAGTCCTC ACTACAAAGA CGTGAGCAAG CCGACGGATG TAGCAACCAA CGTACCCTTC 
CGGGGACAGG CTGTGCGTGA AGTGCCTCCT CCGCTGACTC CGGCAGTGTT CAGGAAATCT 
CTGAACCCTG TCCTCAGCAA GTCCAAAACC CTCGAGACCC CCTCGTCCCA CAGGAAGGGG 
CTGGGGGTCG ACTCGGACGC GGGTGAGATG GGCGCGCTCA GCGCAGCTGA GTGGACCATC 
CCAAAGCCGG CCCTGCAGCT CAGCACCGAC CTCGCCATCG GCAAGCAGTC GTGGACGGTG 
AATTCGGAGG ATTCGGTGGA GAGGATCCCG TTGATGTCCC TGGAGCCCAC TCCCTGCAGC 
TCCTATGACG TGGAGATGAG GCCCTACGTG TGCAGCGTGC TGGTGGAGGA GCAGGGCAAA 
GAGGAGCTGG GCCCCGAGGA GGACCCCACG GTGTACACGT GCATCGAGTG CAGCATCTAC 
TTCAAGAAGA AGGAGCACCT GATGGATCAC ATGCTCCAGC ACAACCGTGG ACCAGGGAGG 
GACCAAGATG GGGACGCTCT CGGAGGGCAG TGCCAGTTCT GCTGCAGTGA GTGCGGGTGG 
GCGTTTGGGG ACCTTGCATC CCTAGAGCAG CACAAAAGGC TCCACCAGGA ATCCAGGGAA 
AAAATCATTG AGGAGATCCA GAAGCTGAAC GAGTTCCCTG ACGAGGGTCG GGAAGCCCGG 
CTGCAGTGCC CCAAGTGCAT CTTTGGCACC AACTCCTCCA AGATCTTCGT GCAGCACGCC 
AAGATGCATG TCAAGGAGAG GAAGGACCAA GGGGCGAAGA GCTTGAATCT CTACGGGGGC 
GGAGAGCTGC AGGATAGCCC CGTGCACGGT ATGTACAAAC ACTTCAAACC CAATGAGCAC 
ATGCCCCTGC AAGTGCAGGC GCCTCCGCAC GGCAAAGGGC TCAGCACCTG CATGCTCTGC 
AGCTTCCCGG CGCCCAACGA GAACATCCTC AAAGAGCACA TGAAATACGC CCATTCCCAC 
CTCTCTTGGG ACGCGGAGGT GTACGAAGAA GATCCCAACC AACCGGGAAC CAGCAGGGAC 
GCCTACAGCC CCGCCAGGCC GGGCCGCTTT GCAGAGACTG ATTATTTTGG CAAAGCAGAC 
CAGCTCTTCC CTCCACCCCA CCGGGAAAGC GCGTCGCATT ACGACATCCA CAGCTTCACC 
GTAGATCACC CCAGACTCAA CAGAAGCAAT GGGGGTAGCA AAAAGGACTA CTCGACGTCG 
GGGTTCCATG CCAGGAAAGC GGCCCCGTAC GCTGCCCGGC ACAAAAACCT GGGGCTCTCC 
TCCACGAAGG CTTATTCACA GTTTGCTCTG CAGCACCTGA AAAAAAAAAC AGCAGCTCAG 
CACCTCGAAG CCGAAGGCGA CGGTCTGAGG GGTCACCCAA CGGGCCTGGA GGAGGTCGGG 
CACAAATGGA TGTTTATCAA CGAGGTGGGG GGCGTGGAAG GGGAGTTCTC CATGACCACG 
GACATGGACT TGATGGAGCA CAGAGTCACC AAACCCGTCA CCATCCCTCA AGCTGCTTTG 
GACCTCAAGA GGACGTACAG AGACACGTTG AGAGCCATGG ACCCTTTGGT CGCCTCCGAA 
GAGCAGCAGC AGCAGCTGCG GATGATGGTG CCCATCGTTC TGCTGGAGGA GGTCAACCCA 
CACCCCAAAG CAAAGAAGCG GCCCCGGGCA AAGCCCTGCA AGAAGAAAGC AGCATTCCCA 
TCGCGGGAGT TCATGATGGA AGAGCCTCTT CCCTTGGATG TGCTCCTGTT GGACTCTCCT 
TTAGAGTTTG ATGACTTTTT GGACTCCGAC TCGCCCATGT TGAAGAACGA GGAGAGGAAG 
TGCCCGTATT GCCCGGATCG GTTCCACAAC GGCATTGGGT TGGCGAACCA CGTGCGGGGC 
CACCTCAATC GGGTTGGTGT GAGCTACAAC GTCCGGCATT TCATCTCGGC AGAAGAAGTG 
AAAGCTATTG AGCAAAAATT TTCCTTCCAA AAGAAGAAGA AAAAAGTTGC AAACTTCGAC 
CCGAGCACGT TCAGCCTGAT GCGATGCGAG TTCTGCGGGG CCGGCTTCGA CACACGCGCC 
GGGCTCTCCA GCCACGCCAG GGCCCACCTG AGAGACTTTG GGATCACCAA CTGGGAGCTC 
ACCATCTCGC CCATCAACAT CCTCAAGGAG CTGTTGGCCA ACTCGGCGGA GTACCCCATG 
CTGCAGGCGG CGGTGGGAGC GGAGCCCTCA TCCCCGGGCC GAGAGCCGCA CGGCTTCGTG 
CCCCGCAAGA GCATGACCCC CATCTCCGAG TGCAGCATTC CACGCTCTCC TCTGTCCCCA 
TTCCCTCCAT CTTGGGGAGA TGAGTCCTTG CAGTCCTACA GAGACGCCCA GAACCTGACG 
ACGTGCGAGG TGTGCGGCGC CTGCTTCGAA ACCCGCAAAG GGCTCTCCAG CCACGCGCGC 
TCCCACCTGC GGCAGCTCGG CGTGGCCGAG TCGGAGAGCA GCGGGGCCCC CATCGATTTG 
CTCTACGAAC TGATGAAGCA GAAGGGCAAA CCCGATGGGC ACCCCATGGC TCCCCCCACC 
CTCAGCAAAA AATCCGCTTC TCCCAAGGAC GGCATTGCCG GCTCCCCGCG GCCCGCGCTG 
CTCGCGCTCG GCAAAGGCGG TGAGCGCCCG CTGGATGGCC CCATCAACAA AGCCATCAAA 
TCCCCGCCAG GATTCTCCAA GAGCCTCTCG CAGCCCGGAT CCCCCATCCT CAAGAAGGTG 
CCCCCCGCCC TCTCCGGGTC CCCCTCTCCG AAAAACCCCG AGGAGAAGAG CTCGAAGCTA 
TCGCTGAGCC CTCTGCAGAG CTCCCCAAAG GCGCAGTGGC CGCAGGCGGA TGAGGAAGGA 
CCTCTCAATT TGACCGCGGG GGGAGAGCCG GTGCGGGACA TCCGCTGCGA GTTCTGCGGA 
GAGTTCTTTG AGAACCGCAA GGGGCTGTCG AGCCACGCGC GCTCCCACCT CCGACAGATG 
GGGGTGACGG AGTGGTACGT CAACGGCTCA CCCATCGACA CGCTGCGCGA GATCCTCAAG 
CGCCGAACCC ACCCGCGGAG CGGAACCTCC AACCCCATGG GCCCCGGGCA CAAAGCCATG 
GCCAAGACCC TCCTGGCAGC CATGGGACCC CTGGAGCCGC GGGGTCCCGG GGAGCTCCAC 
ATCCCGACTC TGCCCAAGAA GGTCCAGCAG CCTGGCAATC CCCTGGGGCA GTCTCCTACC 
TCGTCCCCAC CTCCCACTGC CCGGAAGATG TTTCCAGGCC TCTCTCCTCC CTCCTTGCAG 
AAGAAGCTCA AACATGACCA GATGAGAGTG GAAATCAAGC GTGAGATGAT GACGGGGGGT 
CTCCACGGAG AGCCCCACCC CTCCGACCAG GCTTGGCCAC CGCTGGAGGA GATGTCACCC 
CTGAACCTCT CCTCCCGGGC CGACCCGGTG CGGGACATCC GCTGCGAGTT CTGCGGAGAG 
TTCTTTGAGA ACCGCAAGGG GCTGTCGAGC CACGCGCGCT CCCACCTCCG ACAGATGGGG 
GTGACGGAGT GGTCGGTGAA CGGCTCTCCC ATCGACACGC TGCGCGAGAT CCTCAAGAAG 
AAATCGAAGC CCTGCGTCAT CAAGAAGGAG CCCCACACCT CCATCATTGA AACCCCCAAA 
GGGTTTGGGG AGGAGGGGAT GGACCCCAAG CCCCCCGGAA AAGTGCTGCA GGCCATGGCT 
CTCCCTCCGC TGGGCGGGCG GACGGGGAAA CCCGGTGGCT CCAGCATGGG CCGCGAGCTC 
TCGCTGTCGC CGCTCACCAC CAAGCCCCAC GGTGCCTTCT TGACACCACT GGCCACCAAA 
CGGCCACTGC AGGACGACCG CCTGGGTCCC CACCCCGAGG TGAAGCACAA GGCCTACATC 
CAGACCGAGC TGCCCTTCAA AACCAAGTCC GTACACGACA AGCCCACGCA CACATCCACC 
GAAGCGTGCT GCGAGCTCTG CGGCCTCTAC TTCGAGAACC GCAAGGCTCT GGCCAGCCAC 
GCGCGGGCGC ACCTCCGGCA ATTCGGCGTC ACCGAATGGT GCGTCAACGG CTCTCCCATC 
GAGACGCTCA GCGAATGGAT CCGGCACCGG CCGCAGAAAG CCGGCGCGTA CCGCAGCTAC 
ATCCAGGGGG GCCGACCCTT CACAAAGAAG TTCAGGAATT CTTCGCACGC CCGAGACCAC 
GACGGAGCGC GGAGGATGCC GCTGAGCCTG CAGCACGGCG GCATGGCCCT GCTGAGCAAA 
GGGCTGGCGG CGGAGCTGGC GCACGGGGAG CCCTGCAAGA TCCTGGACGG CACCGGGGAG 
CGGCCCATGG TCACCTCGCC CCTCTCGCTG GTGAAGATGG AAGAACATCA GCGCACGAAT 
TTCCACAAGT TCGAGCGGAG GCAGGCAAGG CCCCTGGATC CCACCCTGCA CAGGGAAGAG 
GAGGGGGCCG ACTTCCAGCA GAAGATGGAG GAGACCCGCC AGCCCCCCCC ANGGATGAGG 
CCGGTGCCCT CGCTGGTCCC CCGCCCGCCA CAGACCTCCC TGGTGAAGTT TGTGGGTAAC 
ATCTACACCC TCAAGTGCAG GTTCTGTGAG GTGGAGTTCC AGGGCCCTCT CTCCATCCAG 
GAGGAGTGGG TGCGACACCT CCAGCGACAC ATCCTGGAAA TGAATTTCTC CAAAGCAGAC 
CCCCTGCGGG GGGACGCCCC GGCCCCCGAA CCCCCCGCCG TGGCCGAGGC TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015158171.1
CDS	297..5933
Translation	MEKAVPTLSEQQRERQGDGSRALLMPLEPNKENFAAPDCPQGEERRAAFVKDAKRPLESAALSGTSVEVGTELDEAAFYTADDLVTMSRDTKGPSGTDLSPLQEDSVFFSPLSRQEPDIALEACDVADEPDPALSGAVPAPAHRTELGSKTEEHLEFQKALYRADARSSLELRAMTVSGSVPWDTSALETRAGDGREESTLERLSSLKDSKDVSAFPGDPQVVNTVRVDPEDLESDAEELGAGMAAAGTPNPCTGPPVGSGEEKYLKPEPKQLEGVGKEHLEKSRRGIQRVDWISRPNRSPSPHYKDVSKPTDVATNVPFRGQAVREVPPPLTPAVFRKSLNPVLSKSKTLETPSSHRKGLGVDSDAGEMGALSAAEWTIPKPALQLSTDLAIGKQSWTVNSEDSVERIPLMSLEPTPCSSYDVEMRPYVCSVLVEEQGKEELGPEEDPTVYTCIECSIYFKKKEHLMDHMLQHNRGPGRDQDGDALGGQCQFCCSECGWAFGDLASLEQHKRLHQESREKIIEEIQKLNEFPDEGREARLQCPKCIFGTNSSKIFVQHAKMHVKERKDQGAKSLNLYGGGELQDSPVHGMYKHFKPNEHMPLQVQAPPHGKGLSTCMLCSFPAPNENILKEHMKYAHSHLSWDAEVYEEDPNQPGTSRDAYSPARPGRFAETDYFGKADQLFPPPHRESASHYDIHSFTVDHPRLNRSNGGSKKDYSTSGFHARKAAPYAARHKNLGLSSTKAYSQFALQHLKKKTAAQHLEAEGDGLRGHPTGLEEVGHKWMFINEVGGVEGEFSMTTDMDLMEHRVTKPVTIPQAALDLKRTYRDTLRAMDPLVASEEQQQQLRMMVPIVLLEEVNPHPKAKKRPRAKPCKKKAAFPSREFMMEEPLPLDVLLLDSPLEFDDFLDSDSPMLKNEERKCPYCPDRFHNGIGLANHVRGHLNRVGVSYNVRHFISAEEVKAIEQKFSFQKKKKKVANFDPSTFSLMRCEFCGAGFDTRAGLSSHARAHLRDFGITNWELTISPINILKELLANSAEYPMLQAAVGAEPSSPGREPHGFVPRKSMTPISECSIPRSPLSPFPPSWGDESLQSYRDAQNLTTCEVCGACFETRKGLSSHARSHLRQLGVAESESSGAPIDLLYELMKQKGKPDGHPMAPPTLSKKSASPKDGIAGSPRPALLALGKGGERPLDGPINKAIKSPPGFSKSLSQPGSPILKKVPPALSGSPSPKNPEEKSSKLSLSPLQSSPKAQWPQADEEGPLNLTAGGEPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWYVNGSPIDTLREILKRRTHPRSGTSNPMGPGHKAMAKTLLAAMGPLEPRGPGELHIPTLPKKVQQPGNPLGQSPTSSPPPTARKMFPGLSPPSLQKKLKHDQMRVEIKREMMTGGLHGEPHPSDQAWPPLEEMSPLNLSSRADPVRDIRCEFCGEFFENRKGLSSHARSHLRQMGVTEWSVNGSPIDTLREILKKKSKPCVIKKEPHTSIIETPKGFGEEGMDPKPPGKVLQAMALPPLGGRTGKPGGSSMGRELSLSPLTTKPHGAFLTPLATKRPLQDDRLGPHPEVKHKAYIQTELPFKTKSVHDKPTHTSTEACCELCGLYFENRKALASHARAHLRQFGVTEWCVNGSPIETLSEWIRHRPQKAGAYRSYIQGGRPFTKKFRNSSHARDHDGARRMPLSLQHGGMALLSKGLAAELAHGEPCKILDGTGERPMVTSPLSLVKMEEHQRTNFHKFERRQARPLDPTLHREEEGADFQQKMEETRQPPPnullMRPVPSLVPRPPQTSLVKFVGNIYTLKCRFCEVEFQGPLSIQEEWVRHLQRHILEMNFSKADPLRGDAPAPEPPAVAEAQ

Target ORF information:

RefSeq Version	XM_015302685.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus RAS protein activator like 3 (RASAL3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015302685.1

ORF Insert Sequence:

ATGGAGAAAG CTGTCCCTAC TTTATCAGAG CAACAGCGAG AGCGCCAGGG AGATGGAAGC 
AGAGCCCTGC TAATGCCACT GGAGCCAAAC AAAGAGAATT TTGCTGCCCC TGATTGCCCC 
CAAGGTGAAG AGAGAAGAGC TGCTTTTGTG AAAGATGCAA AAAGGCCTCT GGAGAGCGCG 
GCTCTATCTG GGACATCGGT GGAAGTGGGG ACGGAGCTCG ACGAAGCCGC CTTCTACACC 
GCTGATGACT TGGTGACAAT GAGCAGGGAC ACAAAGGGAC CCTCCGGCAC CGATCTCTCC 
CCCCTACAAG AGGACTCCGT CTTCTTCTCT CCGCTGTCCC GCCAAGAGCC CGACATTGCC 
CTGGAAGCCT GCGACGTGGC CGATGAGCCT GATCCTGCCC TCTCCGGTGC TGTCCCAGCC 
CCAGCTCACA GGACCGAACT CGGCTCCAAG ACCGAAGAGC ATTTAGAGTT CCAGAAGGCT 
TTGTACAGAG CGGACGCCAG GAGCTCCCTG GAGCTGCGCG CCATGACCGT GTCCGGCAGC 
GTGCCCTGGG ACACCTCCGC CCTGGAGACC CGAGCGGGTG ATGGCAGAGA AGAAAGCACT 
TTAGAGAGAC TCTCTAGCTT GAAGGACTCA AAGGACGTGT CGGCGTTCCC GGGGGATCCC 
CAGGTTGTAA ATACGGTGCG GGTGGATCCT GAGGACCTGG AGAGCGACGC TGAGGAGCTG 
GGCGCCGGCA TGGCGGCAGC TGGGACGCCC AACCCTTGCA CGGGGCCACC GGTGGGTAGT 
GGGGAGGAGA AATACCTTAA ACCTGAACCA AAGCAACTTG AAGGCGTCGG CAAAGAGCAC 
TTAGAGAAAA GCAGGAGGGG GATCCAGAGG GTCGACTGGA TTTCTCGACC CAACAGAAGC 
CCGAGTCCTC ACTACAAAGA CGTGAGCAAG CCGACGGATG TAGCAACCAA CGTACCCTTC 
CGGGGACAGG CTGTGCGTGA AGTGCCTCCT CCGCTGACTC CGGCAGTGTT CAGGAAATCT 
CTGAACCCTG TCCTCAGCAA GTCCAAAACC CTCGAGACCC CCTCGTCCCA CAGGAAGGGG 
CTGGGGGTCG ACTCGGACGC GGGTGAGATG GGCGCGCTCA GCGCAGCTGA GTGGACCATC 
CCAAAGCCGG CCCTGCAGCT CAGCACCGAC CTCGCCATCG GCAAGCAGTC GTGGACGGTG 
AATTCGGAGG ATTCGGTGGA GAGGATCCCG TTGATGTCCC TGGAGCCCAC TCCCTGCAGC 
TCCTATGACG TGGAGATGAG GCCCTACGTG TGCAGCGTGC TGGTGGAGGA GCAGGGCAAA 
GAGGAGCTGG GCCCCGAGGA GGACCCCACG GTGTACACGT GCATCGAGTG CAGCATCTAC 
TTCAAGAAGA AGGAGCACCT GATGGATCAC ATGCTCCAGC ACAACCGTGG ACCAGGGAGG 
GACCAAGATG GGGACGCTCT CGGAGGGCAG TGCCAGTTCT GCTGCAGTGA GTGCGGGTGG 
GCGTTTGGGG ACCTTGCATC CCTAGAGCAG CACAAAAGGC TCCACCAGGA ATCCAGGGAA 
AAAATCATTG AGGAGATCCA GAAGCTGAAC GAGTTCCCTG ACGAGGGTCG GGAAGCCCGG 
CTGCAGTGCC CCAAGTGCAT CTTTGGCACC AACTCCTCCA AGATCTTCGT GCAGCACGCC 
AAGATGCATG TCAAGGAGAG GAAGGACCAA GGGGCGAAGA GCTTGAATCT CTACGGGGGC 
GGAGAGCTGC AGGATAGCCC CGTGCACGGT ATGTACAAAC ACTTCAAACC CAATGAGCAC 
ATGCCCCTGC AAGTGCAGGC GCCTCCGCAC GGCAAAGGGC TCAGCACCTG CATGCTCTGC 
AGCTTCCCGG CGCCCAACGA GAACATCCTC AAAGAGCACA TGAAATACGC CCATTCCCAC 
CTCTCTTGGG ACGCGGAGGT GTACGAAGAA GATCCCAACC AACCGGGAAC CAGCAGGGAC 
GCCTACAGCC CCGCCAGGCC GGGCCGCTTT GCAGAGACTG ATTATTTTGG CAAAGCAGAC 
CAGCTCTTCC CTCCACCCCA CCGGGAAAGC GCGTCGCATT ACGACATCCA CAGCTTCACC 
GTAGATCACC CCAGACTCAA CAGAAGCAAT GGGGGTAGCA AAAAGGACTA CTCGACGTCG 
GGGTTCCATG CCAGGAAAGC GGCCCCGTAC GCTGCCCGGC ACAAAAACCT GGGGCTCTCC 
TCCACGAAGG CTTATTCACA GTTTGCTCTG CAGCACCTGA AAAAAAAAAC AGCAGCTCAG 
CACCTCGAAG CCGAAGGCGA CGGTCTGAGG GGTCACCCAA CGGGCCTGGA GGAGGTCGGG 
CACAAATGGA TGTTTATCAA CGAGGTGGGG GGCGTGGAAG GGGAGTTCTC CATGACCACG 
GACATGGACT TGATGGAGCA CAGAGTCACC AAACCCGTCA CCATCCCTCA AGCTGCTTTG 
GACCTCAAGA GGACGTACAG AGACACGTTG AGAGCCATGG ACCCTTTGGT CGCCTCCGAA 
GAGCAGCAGC AGCAGCTGCG GATGATGGTG CCCATCGTTC TGCTGGAGGA GGTCAACCCA 
CACCCCAAAG CAAAGAAGCG GCCCCGGGCA AAGCCCTGCA AGAAGAAAGC AGCATTCCCA 
TCGCGGGAGT TCATGATGGA AGAGCCTCTT CCCTTGGATG TGCTCCTGTT GGACTCTCCT 
TTAGAGTTTG ATGACTTTTT GGACTCCGAC TCGCCCATGT TGAAGAACGA GGAGAGGAAG 
TGCCCGTATT GCCCGGATCG GTTCCACAAC GGCATTGGGT TGGCGAACCA CGTGCGGGGC 
CACCTCAATC GGGTTGGTGT GAGCTACAAC GTCCGGCATT TCATCTCGGC AGAAGAAGTG 
AAAGCTATTG AGCAAAAATT TTCCTTCCAA AAGAAGAAGA AAAAAGTTGC AAACTTCGAC 
CCGAGCACGT TCAGCCTGAT GCGATGCGAG TTCTGCGGGG CCGGCTTCGA CACACGCGCC 
GGGCTCTCCA GCCACGCCAG GGCCCACCTG AGAGACTTTG GGATCACCAA CTGGGAGCTC 
ACCATCTCGC CCATCAACAT CCTCAAGGAG CTGTTGGCCA ACTCGGCGGA GTACCCCATG 
CTGCAGGCGG CGGTGGGAGC GGAGCCCTCA TCCCCGGGCC GAGAGCCGCA CGGCTTCGTG 
CCCCGCAAGA GCATGACCCC CATCTCCGAG TGCAGCATTC CACGCTCTCC TCTGTCCCCA 
TTCCCTCCAT CTTGGGGAGA TGAGTCCTTG CAGTCCTACA GAGACGCCCA GAACCTGACG 
ACGTGCGAGG TGTGCGGCGC CTGCTTCGAA ACCCGCAAAG GGCTCTCCAG CCACGCGCGC 
TCCCACCTGC GGCAGCTCGG CGTGGCCGAG TCGGAGAGCA GCGGGGCCCC CATCGATTTG 
CTCTACGAAC TGATGAAGCA GAAGGGCAAA CCCGATGGGC ACCCCATGGC TCCCCCCACC 
CTCAGCAAAA AATCCGCTTC TCCCAAGGAC GGCATTGCCG GCTCCCCGCG GCCCGCGCTG 
CTCGCGCTCG GCAAAGGCGG TGAGCGCCCG CTGGATGGCC CCATCAACAA AGCCATCAAA 
TCCCCGCCAG GATTCTCCAA GAGCCTCTCG CAGCCCGGAT CCCCCATCCT CAAGAAGGTG 
CCCCCCGCCC TCTCCGGGTC CCCCTCTCCG AAAAACCCCG AGGAGAAGAG CTCGAAGCTA 
TCGCTGAGCC CTCTGCAGAG CTCCCCAAAG GCGCAGTGGC CGCAGGCGGA TGAGGAAGGA 
CCTCTCAATT TGACCGCGGG GGGAGAGCCG GTGCGGGACA TCCGCTGCGA GTTCTGCGGA 
GAGTTCTTTG AGAACCGCAA GGGGCTGTCG AGCCACGCGC GCTCCCACCT CCGACAGATG 
GGGGTGACGG AGTGGTACGT CAACGGCTCA CCCATCGACA CGCTGCGCGA GATCCTCAAG 
CGCCGAACCC ACCCGCGGAG CGGAACCTCC AACCCCATGG GCCCCGGGCA CAAAGCCATG 
GCCAAGACCC TCCTGGCAGC CATGGGACCC CTGGAGCCGC GGGGTCCCGG GGAGCTCCAC 
ATCCCGACTC TGCCCAAGAA GGTCCAGCAG CCTGGCAATC CCCTGGGGCA GTCTCCTACC 
TCGTCCCCAC CTCCCACTGC CCGGAAGATG TTTCCAGGCC TCTCTCCTCC CTCCTTGCAG 
AAGAAGCTCA AACATGACCA GATGAGAGTG GAAATCAAGC GTGAGATGAT GACGGGGGGT 
CTCCACGGAG AGCCCCACCC CTCCGACCAG GCTTGGCCAC CGCTGGAGGA GATGTCACCC 
CTGAACCTCT CCTCCCGGGC CGACCCGGTG CGGGACATCC GCTGCGAGTT CTGCGGAGAG 
TTCTTTGAGA ACCGCAAGGG GCTGTCGAGC CACGCGCGCT CCCACCTCCG ACAGATGGGG 
GTGACGGAGT GGTCGGTGAA CGGCTCTCCC ATCGACACGC TGCGCGAGAT CCTCAAGAAG 
AAATCGAAGC CCTGCGTCAT CAAGAAGGAG CCCCACACCT CCATCATTGA AACCCCCAAA 
GGGTTTGGGG AGGAGGGGAT GGACCCCAAG CCCCCCGGAA AAGTGCTGCA GGCCATGGCT 
CTCCCTCCGC TGGGCGGGCG GACGGGGAAA CCCGGTGGCT CCAGCATGGG CCGCGAGCTC 
TCGCTGTCGC CGCTCACCAC CAAGCCCCAC GGTGCCTTCT TGACACCACT GGCCACCAAA 
CGGCCACTGC AGGACGACCG CCTGGGTCCC CACCCCGAGG TGAAGCACAA GGCCTACATC 
CAGACCGAGC TGCCCTTCAA AACCAAGTCC GTACACGACA AGCCCACGCA CACATCCACC 
GAAGCGTGCT GCGAGCTCTG CGGCCTCTAC TTCGAGAACC GCAAGGCTCT GGCCAGCCAC 
GCGCGGGCGC ACCTCCGGCA ATTCGGCGTC ACCGAATGGT GCGTCAACGG CTCTCCCATC 
GAGACGCTCA GCGAATGGAT CCGGCACCGG CCGCAGAAAG CCGGCGCGTA CCGCAGCTAC 
ATCCAGGGGG GCCGACCCTT CACAAAGAAG TTCAGGAATT CTTCGCACGC CCGAGACCAC 
GACGGAGCGC GGAGGATGCC GCTGAGCCTG CAGCACGGCG GCATGGCCCT GCTGAGCAAA 
GGGCTGGCGG CGGAGCTGGC GCACGGGGAG CCCTGCAAGA TCCTGGACGG CACCGGGGAG 
CGGCCCATGG TCACCTCGCC CCTCTCGCTG GTGAAGATGG AAGAACATCA GCGCACGAAT 
TTCCACAAGT TCGAGCGGAG GCAGGCAAGG CCCCTGGATC CCACCCTGCA CAGGGAAGAG 
GAGGGGGCCG ACTTCCAGCA GAAGATGGAG GAGACCCGCC AGCCCCCCCC ANGGATGAGG 
CCGGTGCCCT CGCTGGTCCC CCGCCCGCCA CAGACCTCCC TGGTGAAGTT TGTGGGTAAC 
ATCTACACCC TCAAGTGCAG GTTCTGTGAG GTGGAGTTCC AGGGCCCTCT CTCCATCCAG 
GAGGAGTGGG TGCGACACCT CCAGCGACAC ATCCTGGAAA TGAATTTCTC CAAAGCAGAC 
CCCCTGCGGG GGGACGCCCC GGCCCCCGAA CCCCCCGCCG TGGCCGAGGC TCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	RASAL3
Entrez Gene ID	426166
Full Name	RAS protein activator like 3
General protein information	Preferred Names RAS protein activator like 3 Names LOW QUALITY PROTEIN: protein Wiz RAS protein activator like 3
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Mus musculus (house mouse)	Wiz	NP_997603.3
Rattus norvegicus (Norway rat)	Wiz	NP_001101534.1
Homo sapiens (human)	WIZ	NP_067064.2
Pan troglodytes (chimpanzee)	WIZ	XP_003316224.2
	LOC718251	XP_002808223.1
Canis lupus familiaris (dog)	WIZ	XP_005632822.1
Gallus gallus (chicken)	WIZ	XP_004950086.1
Bos taurus (cattle)	WIZ	XP_005208632.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

RASAL3 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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