AGL cDNA ORF clone, Gallus gallus(chicken)

The following AGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa39178 XM_015290748.2
Latest version!
Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39178 XM_015290749.1
Latest version!
Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39178 XM_015290747.2
Latest version!
Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39178 XM_015290745.2
Latest version!
Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39179 XM_015290750.2
Latest version!
Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
View Old Accession Versions >>
OGa39178 XM_015290745.1 Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39179 XM_015290750.1 Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39178 XM_015290748.1 Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa39178 XM_015290747.1 Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290747.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146233.1
    CDS151..4743
    Translation

    Target ORF information:

    RefSeq Version XM_015290747.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146235.1
    CDS149..4741
    Translation

    Target ORF information:

    RefSeq Version XM_015290749.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290745.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146231.1
    CDS244..4836
    Translation

    Target ORF information:

    RefSeq Version XM_015290745.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290745.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290745.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146231.1
    CDS289..4881
    Translation

    Target ORF information:

    RefSeq Version XM_015290745.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290748.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290748.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146234.1
    CDS219..4811
    Translation

    Target ORF information:

    RefSeq Version XM_015290748.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290748.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290748.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146234.1
    CDS223..4815
    Translation

    Target ORF information:

    RefSeq Version XM_015290748.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39178
    Clone ID Related Accession (Same CDS sequence) XM_015290747.1 , XM_015290749.1 , XM_015290745.1 , XM_015290745.2 , XM_015290748.2 , XM_015290748.1 , XM_015290747.2
    Accession Version XM_015290747.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290747.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146233.1
    CDS196..4788
    Translation

    Target ORF information:

    RefSeq Version XM_015290747.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290747.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGGA GATGCTAGCC
    ACAGCCAGAT CAGTCAGACC TAATCTTTAT GTGATAGCTG AACTCTTCAC GGGCAGTGAG
    TATATTGACA ATGTTTTTGT CAACCGTCTG GGAATTACCA GCCTGATTAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39179
    Clone ID Related Accession (Same CDS sequence) XM_015290750.2 , XM_015290750.1
    Accession Version XM_015290750.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006095.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015290750.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGTA CATGCTGGAC
    ACAGCTAGAA AGTTGCGAGC TGATTTGTTT GTAGTGGCTG AACTGTTCAC AGGAAATGAG
    GAGCTGGACA ATATCTTTGT GAATAGTCTG GGCATTACCT CCTTAATAAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146236.1
    CDS219..4811
    Translation

    Target ORF information:

    RefSeq Version XM_015290750.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290750.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGTA CATGCTGGAC
    ACAGCTAGAA AGTTGCGAGC TGATTTGTTT GTAGTGGCTG AACTGTTCAC AGGAAATGAG
    GAGCTGGACA ATATCTTTGT GAATAGTCTG GGCATTACCT CCTTAATAAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39179
    Clone ID Related Accession (Same CDS sequence) XM_015290750.2 , XM_015290750.1
    Accession Version XM_015290750.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product glycogen debranching enzyme isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455911.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGTA CATGCTGGAC
    ACAGCTAGAA AGTTGCGAGC TGATTTGTTT GTAGTGGCTG AACTGTTCAC AGGAAATGAG
    GAGCTGGACA ATATCTTTGT GAATAGTCTG GGCATTACCT CCTTAATAAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015146236.1
    CDS150..4742
    Translation

    Target ORF information:

    RefSeq Version XM_015290750.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015290750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGCCGAGCC ATAAGGAGAC GCGGCTGCTG CACCTCAACG AGATGGAGAA GCTGGACAAG 
    ACCCTTTTCA GGCTGGAGCA AGGGTTTGAA CTGCAGTTTC GACTGGGCCC AACTCTTCAA
    GGCAAGCATG TTACTGTATG TACAAATTAT CCAGCTTCTG GAGAAGTATT TGATCGCCAC
    AAGTTCAGAA CATTATCATG GCACAATCCC ACAGGAAAAG AGGATGACTC TGACAAATAT
    TGCAAGTTAG ATCTCCAAAT TTCTGGGTCG TATCAGTACT ATTTTAATCT TGGAAATGAG
    AAAAGTGGTG GAGGTTACAT AGTTGTGGAT CCTATTTTGC GTGTTGGAGC CGATAACCAT
    GTATTACCTT TGGACTGTGT TACACTCCAG ACATTCCTTG CTAAATGTCT GGGACCATTC
    CACGAATGGG AAAATAGGCT TAAAGTTGCA AAAGAAACAG GTTACAACAT GATTCATTTT
    ACACCGCTGC AGAAGCTTGG ACTGTCTAGA TCATGTTATT CACTGGCTGA TCAGTTAGAA
    GTAAATCCTG AATTTTCAAG CCATAACAAA AAATGCACTT GGAATGATAT AGGAGCTCTT
    GTGGAAAAAA TGAAAAATGA ATGGAATATG CTTTGCATCA CAGATGTAGT CTACAATCAT
    ACTGCTGCTA ACAGCGAGTG GCTCAGGATG CATCCAGAAT GTGGCTACAA CCTTGTAAAT
    TCTCCTCACC TGAAGCCAGC CTGGGTCTTA GATAGAGCTC TGTGGCACCT GAGCTGTATG
    GTGGCTGATG GAAGGTGTAT TGATAAAGGA GTCCCTCCCT TGATTGAAAA TGATCATCAC
    CTGAATTGTA TCCGTAAAAT CATTTGGGAA GATATATATC CAAAAATCAA ACTGTGGGAA
    TTTTTCCAAG TAGATGTTAA TAAAGCTGTG CAGCAATTTA GAACCCTTCT AACTAAAGGC
    AAAATAGGCA CAAAGTCTGA TCCAAATCAA CATCTTCAAA TAGTTCAAGA CCCTGATTAT
    AGAAGATTTG GTTGTACTGT AGATATGAAT ATAGCATTGG CAACATTCAT ACCACACAGC
    AACGGACCAG GTGCAATAGA AGAATGTTGT AACTGGTTTC GCAAGAGAAT TGAGGAACTG
    AATGCTGAAC AATACAGACA TATTCATCAT CATCAAGAGC AGGCTGTCAA CTGTCTTGCA
    GGAACTGTGG TATATGAACG TCTGGCTGGT CATGGTCCTA AACTAGGTCC TATTAGTAGA
    AAATATCCTC TAGTTACCAG GTATTTTACT TATCCATTCA AAGACCTGAC TGTGGAGGAA
    GAACAAAGTA TGATGCATCA GCCAGATAAA GCTTGTTATT TCATGGCCCA TAACGGATGG
    GTTATGGGTG ATGATCCTCT TAGAAACTTT GCAGAACCAG GTTCAAATGT TTACTTGAGA
    AGGGAGCTTA TTTGTTGGGG AGACAGTGTG AAACTGCGCT ATGGTAACAA ACCCGAAGAC
    TGCCCGTACC TCTGGGCACA TATGAAAAAA TACACTGAAA TCACTGCCAA ACATTTCCAT
    GGTGTCCGTC TTGACAACTG TCACTCAACA CCTATTCATG TAGCTGAGTA CATGCTGGAC
    ACAGCTAGAA AGTTGCGAGC TGATTTGTTT GTAGTGGCTG AACTGTTCAC AGGAAATGAG
    GAGCTGGACA ATATCTTTGT GAATAGTCTG GGCATTACCT CCTTAATAAG AGAGGCCATG
    ACAGCTTATA ATAGCCATGA AGAGGGAAGG TTAGTTTATC GTTTTGGAGG TGAACCTGTT
    GGCTCTTTTG TTCAGCCACG TTTGCGGCCC TTGGTGCCAG CAATAGCGCA TGCGCTGTTC
    ATGGATATTA CACATGACAA TGAGTGTCCA ATTCAGCACC GATCTGCATA TGATGCACTT
    CCAAGTGCAA TGATTGTTTC CATGGCATGT TGTGCTACAG GAAGCACCAA AGGTTATGAT
    GAACTAGTAC CACACCAGAT TTCTGTAGTA TCTGAAGAGA GATTTTATTC AACCTGGAAT
    CCACAAGCAC ACCTGAATTC TGGTGAAGTT AATTTCCAGA CAGGAATTCT AGCTGGAAGG
    CTAGCCATGA ACAGGCTGCA TCAGGAGCTG GGAACTAAAG GCTTCAATCA GGTGTATGTA
    GATCAAGTTG ATGAAGATAT AGTGGCAGTG ACAAGACATT GCCCTAACAC ACACCAGTCT
    GTTGTGGCTG TAAGTAGAAC TGCTTTCAGG GATCCAAAGA CTTCCTTCTA CAGTAAAGAA
    GTACCTGAAA TGTGTATCCC AGGAAAAATT GAAGAAGTAG TATTTGAGGC TAGAACTATT
    GAGAGAAGTG TTAGTCCTTA CACAAAAGAT GAACATTTTA TAAATGGATT GCCTAATTTC
    ACAGTGGAAC TCAGAGAGCA TATCCAGATT AAAGACAGTA AAATTATAAA GCAAGCTGGA
    ACTGCCATAA AAGGGCCAAA TGAATTTGTT CAAGAAATAG AATTTGAAAA TTTAACACCA
    GGAAGTGTAA TAGTATTCAG AGTTAGTCTT GATCCAAAGG CACAAGAGGC TGTTGGTGTA
    CTTCGCAATC ATCTGATTCA GTTCAGTCCT CATTTCAAAT CTGGGAGTCT TCCTGATGAT
    CATTCAGCAC CTATATTAAA AACATTATTT TCCTCAATTG CATCTAAACT AACTTTGGCT
    GACTTAAATC AAGTGCTGTA TAGGTGTGAG TCAGAAGAAC AAGAAGATGG TGGAGGTTGT
    TACAATATAC CAAACTGGTC ATCCCTGAAG TATGCAGGCC TCCAAGGGTT AATGTCAGTG
    ATGGCAGACA TTAGACCAAA GAATGATTTG GGACATCCGT TCTGTGATAA TTTAAGATCT
    GGAGACTGGA TGATTGATTA TGTCAGTAAT CGTCTGATTT CACGTGCAGG AGCCTGTGCA
    GAAGTTGGCA AATGGTTGAA GGCCATGTTT GTGTATTTAA AGAAAATTCC ACGTTACCTT
    ATTCCTTGTT ACTTTGATGC CATTTTAGTG GGTGCATACA CAACTCTTCT GGATGTGGGA
    TGGCATCAGA TGTCTAGCTT TGTTCAGAAT GGATCAACAT TTGTTAAACA CCTTTCCTTG
    GGCTCAATCC AGATGTGTGG GATAGGAAAA TATGCGTGTC TGCCAGATCT GTCTCCTTCC
    TTACACGATG TTCCCTATAG GCTGAATGAG ATTACGCATG AGAAAGAACA GTGCTGTGTT
    ACTTTAGCAG CTGGTTTACC TCACTTCTCT TCTGGAATAT TCCGTTCCTG GGGAAGGGAT
    ACCTTTATTG CACTTAGAGG CCTAATGTTA CTTACGGGAC GTTATATAGA AGCAAGAAAC
    ATAATTCTAG CGTTTGGTGG GACTCTACGA CATGGTCTCA TTCCCAACCT GCTTGGCCAG
    GGAACGCATG CCAGATATAA TTGTCGTGAT GCTGTGTGGT GGTGGCTTCA GTGTATCCAG
    GACTACTGTA AGATTGTTCC AAATGGATTA GACATTCTTA GGTGTCCTGT TTCTAGGATG
    TACCCAAGAG ATGATTCTGC TCCTCAGCCT GCAGGCAGTG TGGATCAGCC ACTCTATGAA
    GTAATACAGG AGGCAATGCA ACGACACATG GAAGGCATAA ATTTCAGAGA AAGAAATGCT
    GGTCCACAGA TAGATCAAAA CATGAGAGAC GAAGGTTTTA ATATAATAGC AGGCATTGAC
    CGTGAAACTG GCTTTGTCTT TGGAGGGAAC CGCTTCAATT GCGGCACTTG GATGGATAAA
    ATGGGAGAGA GTGACAGAGC TCGCAACAAG GGAATTCCTG CTACGCCAAG AGATGGCTCT
    GCTGTGGAAA TTGTTGGCTT GTGCAAGTCG GCTGTTCGCT GGTTGCTGGA TTTATCTAGG
    AAAAATGAAT ACCCATTCCA TGGTGTCACC ATAAAAAGAT ACGGAAGGGA GGAAACTATC
    ACATATGATG AATGGAATAG AAAAATTCAA GGACACTTTG AAAGACTCTT CTTTGTCTCT
    GAGAACCCTG CAGATCAAAA TGAAAAACAT CCAAATCTAG TTCACAAACG TGGGATTTAT
    AAAGACAGCT ATGGAGCTTC AAGTCCATGG TGTGATTACC AACTCAGGCC AAATTTCACA
    ATAGCAATGG TTGTGGCCCC TGAGTTGTTC ACACCTGAGA GAGCTTGGAA AGCTCTGAGG
    ATAGCAGAGG AAAAACTACT TGGTCCATTG GGCATGAAAA CTTTAGATCC AGATGATATG
    GTGTACTGTG GCATATATGA TAACGCTCTT GACAATGACA ACTATAATGT TGCCAGAGGT
    TTTAATTACC ACCAAGGACC TGAATGGCTG TGGCCGATCG GATATTTTCT TCGTGCCAAA
    TTGTACTTCT CAAAGTTGAT TGGTCCAGAG ATATATGCAA AAACTGTAGT TATGATAAAG
    AATGTTCTTT CTCGCCACTA TGTTCACCTT GAAAGATCAT CCTGGAAAGG GCTTCCAGAA
    CTGACCAATG AAAATGGGCA GTATTGTCCT TTTAGCTGTG AAACTCAGGC CTGGTCGATT
    GGTGTTATCC TTGAAGTTCT TTATGACTTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.