FBXO32 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	FBXO32
Entrez Gene ID	420343
Full Name	F-box protein 32
General protein information	Preferred Names F-box protein 32 Names F-box only protein 32 atrogin-1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015283108.2	XP_015138594.1	F-box only protein 32 isoform X1
XM_015283108.1	XP_015138594.1	F-box only protein 32 isoform X1
NM_001030956.1	NP_001026127.1	F-box only protein 32

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fbxo32	NP_598205.1
	FBXO32	XP_001100697.2
Bos taurus (cattle)	FBXO32	NP_001039620.1
Danio rerio (zebrafish)	fbxo32	NP_957211.1
Xenopus tropicalis (tropical clawed frog)	fbxo32	XP_002941443.1
Canis lupus familiaris (dog)	FBXO32	XP_532324.2
Gallus gallus (chicken)	FBXO32	NP_001026127.1
Homo sapiens (human)	FBXO32	NP_478136.1
Pan troglodytes (chimpanzee)	FBXO32	XP_519940.2
Mus musculus (house mouse)	Fbxo32	NP_080622.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of first exogenous nutrients on the mRNA levels of atrogin-1/MAFbx and GLUT1 in the skeletal muscles of newly hatched chicks.
Ijiri D, Shimamoto S, Kawaguchi M, Furukawa A, Nakashima K, Tada O, Ohtsuka A
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology2058-14(2017 Mar)

Effect of dexamethasone on the expression of atrogin-1/MAFbx in chick skeletal muscle.
Nakashima K, Ishida A, Ijiri D, Ohtsuka A
Animal science journal = Nihon chikusan Gakkaiho87(3)405-10(2016 Mar)

Comparison of atrogin-1/MAFbx mRNA expression in the gizzards of egg- and meat-type chickens.
Nakashima K, Ishida A, Katsumata M
Bioscience, biotechnology, and biochemistry77(11)2319-21(2013)

Atrogin-1/MAFbx, a muscle-specific ubiquitin ligase, is highly expressed in the smooth muscle of the chicken gizzard.
Nakashima K, Ishida A, Katsumata M
Bioscience, biotechnology, and biochemistry77(5)1092-5(2013)

Regulators of protein metabolism are affected by cyclical nutritional treatments with diets varying in protein and energy content.
Boussaid-Om Ezzine S, M?tayer-Coustard S, Collin A, Rideau N, Leterrier C, Bouvarel I, Seiliez I, Tesseraud S
The Journal of nutritional biochemistry23(11)1467-73(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

chicks having free access to feed for the first 24h increased their body weight and had decreased atrogin-1/MAFbx mRNA levels in their sartorius and pectoralis major muscles compared with the fasted chicks
Title: Effects of first exogenous nutrients on the mRNA levels of atrogin-1/MAFbx and GLUT1 in the skeletal muscles of newly hatched chicks.

Its enhanced expression causes muscle atrophy.
Title: Effect of dexamethasone on the expression of atrogin-1/MAFbx in chick skeletal muscle.

Atrogin-1/MAFbx mRNA expression in the smooth muscle of the gizzard was higher in meat-type chickens than in egg-type chickens.
Title: Comparison of atrogin-1/MAFbx mRNA expression in the gizzards of egg- and meat-type chickens.

atrogin-1/MAFbx mRNA is highly expressed in the smooth muscle of the chicken gizzard.
Title: Atrogin-1/MAFbx, a muscle-specific ubiquitin ligase, is highly expressed in the smooth muscle of the chicken gizzard.

The expression of atrogin-1 was regulated with diets varying in protein and energy content.
Title: Regulators of protein metabolism are affected by cyclical nutritional treatments with diets varying in protein and energy content.

The following FBXO32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FBXO32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa26650	NM_001030956.1 Latest version!	Gallus gallus F-box protein 32 (FBXO32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OGa26650	XM_015283108.2 Latest version!	Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
OGa26650	XM_015283108.1	Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FBXO32 ( NM_001030956.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001026127.1 Gallus gallus F-box protein 32 (FBXO32), mRNA.

Price & Availability↑

CloneID	OGa26650
Clone ID Related Accession (Same CDS sequence)	XM_015283108.2 , XM_015283108.1 , NM_001030956.1
Accession Version	NM_001030956.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	F-box only protein 32
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ721108.1. On Aug 8, 2005 this sequence version replaced XM_418450.1. ##Evidence-Data-START## Transcript exon combination :: AJ721108.1 [ECO:0000332] ##Evidence-Data-END##

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026127.1
CDS	77..1144
Translation	MPFLGQDWRSPGQSWVKTADGWTRFLDEKSGGFVGDLSSFCKKEDHNKENLFNSLNYDVAAKKRKKDLLNNKAKIQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSSAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNYMNILEKVVQKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVHRMETILHWQQQLNNIQITRPAFKGTTFTDLPLCLQLNIMQRLSDGRDLVSLGQVAPDLQVLSEDRLLWKKLCQYHFTDRQIRKRLILSDKGQLDWKKMYFKLIRCYPRKEQYGDTLQLCRHCHILSWKGTDHPCTANNPETCSTSLSPQDFINLFRF

Target ORF information:

RefSeq Version	NM_001030956.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus F-box protein 32 (FBXO32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030956.1

ORF Insert Sequence:

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO32 ( XM_015283108.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015138594.1 Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa26650
Clone ID Related Accession (Same CDS sequence)	XM_015283108.2 , XM_015283108.1 , NM_001030956.1
Accession Version	XM_015283108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	F-box only protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015283108.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015138594.1
CDS	77..1144
Translation	MPFLGQDWRSPGQSWVKTADGWTRFLDEKSGGFVGDLSSFCKKEDHNKENLFNSLNYDVAAKKRKKDLLNNKAKIQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSSAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNYMNILEKVVQKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVHRMETILHWQQQLNNIQITRPAFKGTTFTDLPLCLQLNIMQRLSDGRDLVSLGQVAPDLQVLSEDRLLWKKLCQYHFTDRQIRKRLILSDKGQLDWKKMYFKLIRCYPRKEQYGDTLQLCRHCHILSWKGTDHPCTANNPETCSTSLSPQDFINLFRF

Target ORF information:

RefSeq Version	XM_015283108.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283108.2

ORF Insert Sequence:

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FBXO32 ( XM_015283108.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015138594.1 Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa26650
Clone ID Related Accession (Same CDS sequence)	XM_015283108.2 , XM_015283108.1 , NM_001030956.1
Accession Version	XM_015283108.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	F-box only protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455825.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015138594.1
CDS	155..1222
Translation	MPFLGQDWRSPGQSWVKTADGWTRFLDEKSGGFVGDLSSFCKKEDHNKENLFNSLNYDVAAKKRKKDLLNNKAKIQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSSAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNYMNILEKVVQKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVHRMETILHWQQQLNNIQITRPAFKGTTFTDLPLCLQLNIMQRLSDGRDLVSLGQVAPDLQVLSEDRLLWKKLCQYHFTDRQIRKRLILSDKGQLDWKKMYFKLIRCYPRKEQYGDTLQLCRHCHILSWKGTDHPCTANNPETCSTSLSPQDFINLFRF

Target ORF information:

RefSeq Version	XM_015283108.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus F-box protein 32 (FBXO32), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015283108.1

ORF Insert Sequence:

ATGCCGTTCC TCGGGCAGGA CTGGCGCTCC CCTGGGCAGA GCTGGGTGAA GACAGCCGAC 
GGTTGGACGC GCTTTCTCGA TGAGAAGAGC GGCGGTTTCG TCGGCGACCT CAGCAGCTTT 
TGTAAGAAGG AAGATCACAA CAAAGAGAAT CTCTTCAACA GCCTTAACTA TGATGTTGCT 
GCCAAGAAGA GGAAAAAAGA CCTACTGAAT AACAAGGCCA AAATTCAGTA TTTTCATCAA 
GAAAAGTGGA TCTATGTTCA CAAGGGGAGC ACAAAAGAGC GCCATGGTTA CTGCACCTTG 
GGAGAAGCCT TCAACAGACT TGACTTCTCC AGTGCTATCC TGGACTCCCG GCGATTCAAC 
TACGTTGTAA GGCTGTTGGA GCTGATAGCA AAGTCTCAGC TGACGTCACT GAGTGGCATT 
GCCCAGAAAA ACTACATGAA CATTTTGGAA AAAGTGGTGC AGAAAGTCCT TGAAGACCAG 
CAGAACATCA GGCTAATACG GGAATTGCTG CAGACCCTCT ACACGTCCCT CTGCACTTTG 
GTTCAACGGG TCGGCAAGTC TGTCCTTGTA GGAAACATCA ACATGTGGGT GCACAGGATG 
GAGACTATTC TCCACTGGCA GCAGCAACTG AACAACATTC AAATCACCAG GCCTGCCTTT 
AAAGGAACTA CCTTCACAGA CCTGCCATTG TGTTTACAAT TGAACATCAT GCAACGACTG 
AGCGATGGGA GGGACCTGGT TAGCCTTGGT CAAGTGGCTC CCGACCTGCA AGTGCTCAGC 
GAAGACCGGC TGCTGTGGAA GAAGCTCTGC CAGTACCACT TCACAGACAG ACAGATTCGC 
AAACGGCTAA TCCTATCTGA CAAGGGACAG CTGGATTGGA AGAAGATGTA CTTCAAGCTC 
ATAAGGTGTT ACCCACGGAA GGAGCAGTAT GGTGACACGC TGCAGCTGTG CAGGCACTGC 
CACATCCTCT CTTGGAAGGG TACTGATCAC CCTTGCACAG CCAACAACCC AGAGACCTGT 
TCCACCTCTC TTTCACCTCA AGACTTTATC AACTTGTTCA GATTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of first exogenous nutrients on the mRNA levels of atrogin-1/MAFbx and GLUT1 in the skeletal muscles of newly hatched chicks.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology2058-14(2017 Mar)
Ijiri D,Shimamoto S,Kawaguchi M,Furukawa A,Nakashima K,Tada O,Ohtsuka A

Effect of dexamethasone on the expression of atrogin-1/MAFbx in chick skeletal muscle.
Animal science journal = Nihon chikusan Gakkaiho87(3)405-10(2016 Mar)
Nakashima K,Ishida A,Ijiri D,Ohtsuka A

Comparison of atrogin-1/MAFbx mRNA expression in the gizzards of egg- and meat-type chickens.
Bioscience, biotechnology, and biochemistry77(11)2319-21(2013)
Nakashima K,Ishida A,Katsumata M

Atrogin-1/MAFbx, a muscle-specific ubiquitin ligase, is highly expressed in the smooth muscle of the chicken gizzard.
Bioscience, biotechnology, and biochemistry77(5)1092-5(2013)
Nakashima K,Ishida A,Katsumata M

Regulators of protein metabolism are affected by cyclical nutritional treatments with diets varying in protein and energy content.
The Journal of nutritional biochemistry23(11)1467-73(2012 Nov)
Boussaid-Om Ezzine S,M?tayer-Coustard S,Collin A,Rideau N,Leterrier C,Bouvarel I,Seiliez I,Tesseraud S

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FBXO32 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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