Fbxo32 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbxo32
Entrez Gene ID	171043
Full Name	F-box protein 32
Synonyms	Atrogin1,MAFbx
General protein information	Preferred Names F-box protein 32 Names F-box only protein 32 atrogin-1 atrophy gene 1 muscle atrophy F-box protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q33
Summary	may act as an SCF-type E3 ubiquitin ligase; plays a role in skeletal atrophy [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_133521.1	NP_598205.1	F-box only protein 32

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Fbxo32	NP_598205.1
	FBXO32	XP_001100697.2
Bos taurus (cattle)	FBXO32	NP_001039620.1
Danio rerio (zebrafish)	fbxo32	NP_957211.1
Xenopus tropicalis (tropical clawed frog)	fbxo32	XP_002941443.1
Canis lupus familiaris (dog)	FBXO32	XP_532324.2
Gallus gallus (chicken)	FBXO32	NP_001026127.1
Homo sapiens (human)	FBXO32	NP_478136.1
Pan troglodytes (chimpanzee)	FBXO32	XP_519940.2
Mus musculus (house mouse)	Fbxo32	NP_080622.1

Gene Ontology
Bibliography

Related articles in PubMed

Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.
Kang SH, Lee HA, Kim M, Lee E, Sohn UD, Kim I
American journal of physiology. Endocrinology and metabolism312(6)E495-E507(2017 Jun)

Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.
Gerlinger-Romero F, Yonamine CY, Junior DC, Esteves JV, Machado UF
Molecular and cellular biochemistry427(1-2)187-199(2017 Mar)

l-Carnitine supplement reduces skeletal muscle atrophy induced by prolonged hindlimb suspension in rats.
Jang J, Park J, Chang H, Lim K
Applied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme41(12)1240-1247(2016 Dec)

Adenosine monophosphate-activated protein kinase attenuates cardiomyocyte hypertrophy through regulation of FOXO3a/MAFbx signaling pathway.
Chen B, Wu Q, Xiong Z, Ma Y, Yu S, Chen D, Huang S, Dong Y
Acta biochimica et biophysica Sinica48(9)827-32(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that FOXO3a plays a role in muscle atrophy through regulation of expression of MuRF1 and atrogin-1 in skeletal muscle in Cushing's syndrome. (FOXO3a = forkhead box protein O3a; MuRF1 = muscle-specific RING finger protein 1)
Title: Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome.

Independent of the metabolic condition, after muscle contraction, both TRIM63 and FBXO32 proteins correlated significantly with miR-1-3p, miR-29a/b-3p, and miR-133a/b-3p.
Title: Dysregulation between TRIM63/FBXO32 expression and soleus muscle wasting in diabetic rats: potential role of miR-1-3p, -29a/b-3p, and -133a/b-3p.

Data suggest that expression of atrogin-1/Fbxo32 and E3 ubiquitin ligase/parkin protein can be down-regulated by dietary factors; here, L-carnitine dietary supplementation represses expression of these two ligases in skeletal muscle in a model of disuse muscle atrophy due to weightlessness (hindlimb suspension).
Title: l-Carnitine supplement reduces skeletal muscle atrophy induced by prolonged hindlimb suspension in rats.

AMPK plays an important role in the inhibition of cardiomyocyte hypertrophy by activating protein degradation via FOXO3a/MAFbx signaling pathway.
Title: Adenosine monophosphate-activated protein kinase attenuates cardiomyocyte hypertrophy through regulation of FOXO3a/MAFbx signaling pathway.

The muscles also showed significantly contrasting transcriptional regulation of the microRNAs miR-1 and miR-206. MuRF1 and Atrogin-1 showed the highest levels of expression in the denervated gastrocnemius muscle
Title: Investigation of the Expression of Myogenic Transcription Factors, microRNAs and Muscle-Specific E3 Ubiquitin Ligases in the Medial Gastrocnemius and Soleus Muscles following Peripheral Nerve Injury.

The following Fbxo32 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbxo32 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12541	NM_133521.1 Latest version!	Rattus norvegicus F-box protein 32 (Fbxo32), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbxo32 ( NM_133521.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598205.1 Rattus norvegicus F-box protein 32 (Fbxo32), mRNA.

Price & Availability↑

CloneID	ORa12541
Clone ID Related Accession (Same CDS sequence)	NM_133521.1
Accession Version	NM_133521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	F-box only protein 32
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY059628.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY059628.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGTTCC TTGGTCAGGA CTGGCGGTCC CCGGGCCAGA GCTGGGTGAA GACGGCGGAC 
GGCTGGAAGC GCTTCTTGGA TGAGAAAAGC GGCACCTTCG TGAGCGACCT CAGCAGTTAC 
TGCAACAAGG AGAATCTGTT CAACAGCCTG AACTACGATG TTGCAGCCAA GAAGAGAAAG 
AAAGACATAC AGAACAGCAA AACCAAAACT CAGTATTTCC ATCAGGAGAA GTGGATCTAT 
GTTCACAAAG GGAGTACTAA GGAGCGCCAT GGATACTGCA CTTTGGGGGA AGCTTTCAAC 
AGACTGGACT TCTCGACTGC CATCCTGGAT TCCAGAAGAT TCAACTACGT AGTAAGGCTG 
TTGGAGCTGA TAGCAAAGTC ACAGCTCACA TCCCTGAGTG GCATCGCCCA AAAGAACTTC 
ATGAACATTT TGGAAAAAGT AGTACTGAAA GTTCTTGAAG ACCAGCAAAA CATAAGACTC 
ATACGGGAAC TTCTCCAGAC CCTCTACACA TCCTTATGCA CGCTGGTCCA GAGAGTCGGC 
AAGTCCGTGC TGGTGGGCAA CATCAACATG TGGGTGTATC GAATGGAGAC CACTCTACAC 
TGGCAACAGC AGCTGAACAG CATCCAGATC AGCAGGCCGG CCTTCAAAGG TCTCACGATC 
ACCGACCTGC CTGTGTGCTT ACAACTGAAC ATCATGCAGA GGCTGAGCGA TGGGCGGGAC 
CTGGTCAGCC TGGGCCAGGC AGCCCCAGAC CTGCATGTGC TCAGTGAAGA CCGGCTACTG 
TGGAAGAGAC TCTGCCAGTA CCACTTCTCA GAGCGGCAGA TCCGCAAGCG ATTGATCTTG 
TCTGACAAAG GGCAGCTGGA TTGGAAGAAG ATGTACTTTA AGCTTGTGCG ATGTTACCCA 
AGAAGAGAAC AGTATGGGGT CACCCTGCAG CTTTGCAAAC ACTGCCACAT TCTCTCCTGG 
AAGGGCACTG ACCATCCATG CACGGCCAAC AACCCAGAGA GCTGCTCCGT CTCACTTTCA 
CCCCAAGACT TTATTAACTT GTTCAAGTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598205.1
CDS	1..1053
Translation	MPFLGQDWRSPGQSWVKTADGWKRFLDEKSGTFVSDLSSYCNKENLFNSLNYDVAAKKRKKDIQNSKTKTQYFHQEKWIYVHKGSTKERHGYCTLGEAFNRLDFSTAILDSRRFNYVVRLLELIAKSQLTSLSGIAQKNFMNILEKVVLKVLEDQQNIRLIRELLQTLYTSLCTLVQRVGKSVLVGNINMWVYRMETTLHWQQQLNSIQISRPAFKGLTITDLPVCLQLNIMQRLSDGRDLVSLGQAAPDLHVLSEDRLLWKRLCQYHFSERQIRKRLILSDKGQLDWKKMYFKLVRCYPRREQYGVTLQLCKHCHILSWKGTDHPCTANNPESCSVSLSPQDFINLFKF

Target ORF information:

RefSeq Version	NM_133521.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus F-box protein 32 (Fbxo32), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133521.1

ORF Insert Sequence:

ATGCCGTTCC TTGGTCAGGA CTGGCGGTCC CCGGGCCAGA GCTGGGTGAA GACGGCGGAC 
GGCTGGAAGC GCTTCTTGGA TGAGAAAAGC GGCACCTTCG TGAGCGACCT CAGCAGTTAC 
TGCAACAAGG AGAATCTGTT CAACAGCCTG AACTACGATG TTGCAGCCAA GAAGAGAAAG 
AAAGACATAC AGAACAGCAA AACCAAAACT CAGTATTTCC ATCAGGAGAA GTGGATCTAT 
GTTCACAAAG GGAGTACTAA GGAGCGCCAT GGATACTGCA CTTTGGGGGA AGCTTTCAAC 
AGACTGGACT TCTCGACTGC CATCCTGGAT TCCAGAAGAT TCAACTACGT AGTAAGGCTG 
TTGGAGCTGA TAGCAAAGTC ACAGCTCACA TCCCTGAGTG GCATCGCCCA AAAGAACTTC 
ATGAACATTT TGGAAAAAGT AGTACTGAAA GTTCTTGAAG ACCAGCAAAA CATAAGACTC 
ATACGGGAAC TTCTCCAGAC CCTCTACACA TCCTTATGCA CGCTGGTCCA GAGAGTCGGC 
AAGTCCGTGC TGGTGGGCAA CATCAACATG TGGGTGTATC GAATGGAGAC CACTCTACAC 
TGGCAACAGC AGCTGAACAG CATCCAGATC AGCAGGCCGG CCTTCAAAGG TCTCACGATC 
ACCGACCTGC CTGTGTGCTT ACAACTGAAC ATCATGCAGA GGCTGAGCGA TGGGCGGGAC 
CTGGTCAGCC TGGGCCAGGC AGCCCCAGAC CTGCATGTGC TCAGTGAAGA CCGGCTACTG 
TGGAAGAGAC TCTGCCAGTA CCACTTCTCA GAGCGGCAGA TCCGCAAGCG ATTGATCTTG 
TCTGACAAAG GGCAGCTGGA TTGGAAGAAG ATGTACTTTA AGCTTGTGCG ATGTTACCCA 
AGAAGAGAAC AGTATGGGGT CACCCTGCAG CTTTGCAAAC ACTGCCACAT TCTCTCCTGG 
AAGGGCACTG ACCATCCATG CACGGCCAAC AACCCAGAGA GCTGCTCCGT CTCACTTTCA 
CCCCAAGACT TTATTAACTT GTTCAAGTTC TGA