BCO1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCO1
Entrez Gene ID	395346
Full Name	beta-carotene oxygenase 1
Synonyms	BCDO,BCMO1
General protein information	Preferred Names beta-carotene oxygenase 1 Names beta,beta-carotene 15,15'-dioxygenase beta,beta-carotene 15,15'-monooxygenase beta-carotene 15,15'-dioxygenase beta-carotene 15,15'-monooxygenase 1 beta-carotene dioxygenase 1
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
NM_204635.1	NP_989966.1	beta,beta-carotene 15,15'-monooxygenase
XM_015292519.1	XP_015148005.1	beta,beta-carotene 15,15'-monooxygenase isoform X1
XM_015292520.1	XP_015148006.1	beta,beta-carotene 15,15'-monooxygenase isoform X1
NM_001364902.1	NP_001351831.1	beta,beta-carotene 15,15'-dioxygenase

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCMO1	NP_059125.2
Canis lupus familiaris (dog)	BCMO1	XP_546815.1
Gallus gallus (chicken)	BCMO1	NP_989966.1
Danio rerio (zebrafish)	bcmo1l	NP_956902.1
Arabidopsis thaliana (thale cress)	CCD8	NP_195007.2
Oryza sativa (rice)	Os01g0746400	NP_001044229.2
Magnaporthe oryzae (rice blast fungus)	MGG_08016	XP_003714968.1
Bos taurus (cattle)	BCMO1	NP_001019730.1
Mus musculus (house mouse)	Bcmo1	NP_067461.2
Rattus norvegicus (Norway rat)	Bcmo1	NP_446100.2
Caenorhabditis elegans (roundworm)	bcmo-1	NP_496729.2
Caenorhabditis elegans (roundworm)	bcmo-2	NP_494694.2
Neurospora crassa	NCU11424	XP_001727958.1
Pan troglodytes (chimpanzee)	BCMO1	XP_523435.3
Xenopus tropicalis (tropical clawed frog)	bcmo1	XP_002938186.1

Gene Ontology
Bibliography

Related articles in PubMed

Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production.
Praud C, Al Ahmadieh S, Voldoire E, Le Vern Y, Godet E, Courouss? N, Graulet B, Le Bihan Duval E, Berri C, Duclos MJ
Experimental cell research358(2)140-146(2017 09)

Nutrigenetics of carotenoid metabolism in the chicken: a polymorphism at the ?,?-carotene 15,15'-mono-oxygenase 1 (BCMO1) locus affects the response to dietary ?-carotene.
Jlali M, Graulet B, Chauveau-Duriot B, Godet E, Praud C, Nunes CS, Le Bihan-Duval E, Berri C, Duclos MJ
The British journal of nutrition111(12)2079-88(2014 Jun)

A mutation in the promoter of the chicken ?,?-carotene 15,15'-monooxygenase 1 gene alters xanthophyll metabolism through a selective effect on its mRNA abundance in the breast muscle.
Jlali M, Graulet B, Chauveau-Duriot B, Chabault M, Godet E, Leroux S, Praud C, Le Bihan-Duval E, Duclos MJ, Berri C
Journal of animal science90(12)4280-8(2012 Dec)

Detection of a Cis [corrected] eQTL controlling BCMO1 gene expression leads to the identification of a QTG for chicken breast meat color.
Le Bihan-Duval E, Nadaf J, Berri C, Pitel F, Graulet B, Godet E, Leroux SY, Demeure O, Lagarrigue S, Duby C, Cogburn LA, Beaumont CM, Duclos MJ
PloS one6(7)e14825(2011)

Developmental changes in gene expressions of beta-carotene cleavage enzyme and retinoic acid synthesizing enzymes in the chick duodenum.
Yamaguchi N, Yamamoto T, Suruga K, Takase S
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology148(3)690-7(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

observed that in myoblast showing decreased BCO1 expression consecutive to a natural mutation or to a siRNA treatment
Title: Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production.

genetic association study in domestic chickens in France: Data suggest that two SNPs in BCMO1 promoter are associated with higher absorption of dietary beta-carotene in duodenum and reduced absorption of xanthophylls/liposoluble vitamins/cholesterol.
Title: Nutrigenetics of carotenoid metabolism in the chicken: a polymorphism at the ?,?-carotene 15,15'-mono-oxygenase 1 (BCMO1) locus affects the response to dietary ?-carotene.

These results indicated that a genetic variant of BCMO1 specifically changes lutein and zeaxanthin content in the chicken plasma and breast muscle without impairing vitamin A and E metabolism.
Title: A mutation in the promoter of the chicken ?,?-carotene 15,15'-monooxygenase 1 gene alters xanthophyll metabolism through a selective effect on its mRNA abundance in the breast muscle.

Data indicate taht two fully-linked single nucleotide polymorphisms (SNP) located within the proximal promoter of BCMO1 gene were identified.
Title: Detection of a Cis [corrected] eQTL controlling BCMO1 gene expression leads to the identification of a QTG for chicken breast meat color.

Postnatal induction of BCMO1 gene expression in the chick duodenum may be caused by the elevation of serum hydrocortisone levels and may contribute to the RALDH1-mediated retinoic acid synthetic pathway.
Title: Developmental changes in gene expressions of beta-carotene cleavage enzyme and retinoic acid synthesizing enzymes in the chick duodenum.

The following BCO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa00897	NM_204635.1 Latest version!	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa00897	NM_001364902.1 Latest version!	Gallus gallus beta-carotene oxygenase 1 (BCO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OGa41016	XM_015292519.1 Latest version!	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00
OGa41016	XM_015292520.1 Latest version!	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCO1 ( NM_204635.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_989966.1 Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), mRNA.

Price & Availability↑

CloneID	OGa00897
Clone ID Related Accession (Same CDS sequence)	NM_204635.1 , NM_001364902.1
Accession Version	NM_204635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-27
Organism	Gallus gallus(chicken)
Product	beta,beta-carotene 15,15'-monooxygenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN02032321.1 and AADN02032323.1. On Jan 22, 2011 this sequence version replaced XM_414163.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TATGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_989966.1
CDS	97..1677
Translation	METIFNRNKEEHPEPIKAEVQGQLPTWLQGVLLRNGPGMHTIGDTKYNHWFDGLALLHSFTFKNGEVYYRSKYLRSDTYNCNIEANRIVVSEFGTMAYPDPCKNIFAKAFSYLSHTIPEFTDNCLINIMKTGDDYYATSETNFIRKIDPQTLETLDKVDYSKYVAVNLATSHPHYDSAGNILNMGTSIVDKGRTKYVLFKIPSSVPEKEKKKSCFKHLEVVCSIPSRSLLQPSYYHSFGITENYIVFIEQPFKLDIVKLATAYIRGVNWASCLSFHKEDKTWFHFVDRKTKKEVSTKFYTDALVLYHHINAYEEDGHVVFDIVAYRDNSLYDMFYLKKLDKDFEVNNKLTSIPTCKRFVVPLQYDKDAEVGSNLVKLPTSATAVKEKDGSIYCQPEILCEGIELPRVNYDYNGKKYKYVYATEVQWSPVPTKIAKLNVQTKEVLHWGEDHCWPSEPIFVPSPDAREEDEGVVLTCVVVSEPNKAPFLLILDAKTFKELGRATVNVEMHLDLHGMFIPQNDLGAETE

Target ORF information:

RefSeq Version	NM_204635.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_204635.1

ORF Insert Sequence:

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TATGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCO1 ( NM_001364902.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_001351831.1 Gallus gallus beta-carotene oxygenase 1 (BCO1), mRNA.

Price & Availability↑

CloneID	OGa00897
Clone ID Related Accession (Same CDS sequence)	NM_204635.1 , NM_001364902.1
Accession Version	NM_001364902.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Gallus gallus(chicken)
Product	beta,beta-carotene 15,15'-dioxygenase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AADN05000558.1. On Jul 13, 2018 this sequence version replaced XM_015292519.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: ERR2365565.153133.1, ERR2368565.224670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2201374, SAMEA2201375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TATGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351831.1
CDS	137..1717
Translation	METIFNRNKEEHPEPIKAEVQGQLPTWLQGVLLRNGPGMHTIGDTKYNHWFDGLALLHSFTFKNGEVYYRSKYLRSDTYNCNIEANRIVVSEFGTMAYPDPCKNIFAKAFSYLSHTIPEFTDNCLINIMKTGDDYYATSETNFIRKIDPQTLETLDKVDYSKYVAVNLATSHPHYDSAGNILNMGTSIVDKGRTKYVLFKIPSSVPEKEKKKSCFKHLEVVCSIPSRSLLQPSYYHSFGITENYIVFIEQPFKLDIVKLATAYIRGVNWASCLSFHKEDKTWFHFVDRKTKKEVSTKFYTDALVLYHHINAYEEDGHVVFDIVAYRDNSLYDMFYLKKLDKDFEVNNKLTSIPTCKRFVVPLQYDKDAEVGSNLVKLPTSATAVKEKDGSIYCQPEILCEGIELPRVNYDYNGKKYKYVYATEVQWSPVPTKIAKLNVQTKEVLHWGEDHCWPSEPIFVPSPDAREEDEGVVLTCVVVSEPNKAPFLLILDAKTFKELGRATVNVEMHLDLHGMFIPQNDLGAETE

Target ORF information:

RefSeq Version	NM_001364902.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus beta-carotene oxygenase 1 (BCO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364902.1

ORF Insert Sequence:

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TATGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCO1 ( XM_015292519.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148005.1 Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41016
Clone ID Related Accession (Same CDS sequence)	XM_015292519.1 , XM_015292520.1
Accession Version	XM_015292519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	beta,beta-carotene 15,15'-monooxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TACGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148005.1
CDS	210..1790
Translation	METIFNRNKEEHPEPIKAEVQGQLPTWLQGVLLRNGPGMHTIGDTKYNHWFDGLALLHSFTFKNGEVYYRSKYLRSDTYNCNIEANRIVVSEFGTMAYPDPCKNIFAKAFSYLSHTIPEFTDNCLINIMKTGDDYYATSETNFIRKIDPQTLETLDKVDYSKYVAVNLATSHPHYDSAGNILNMGTSIVDKGRTKYVLFKIPSSVPEKEKKKSCFKHLEVVCSIPSRSLLQPSYYHSFGITENYIVFIEQPFKLDIVKLATAYIRGVNWASCLSFHKEDKTWFHFVDRKTKKEVSTKFYTDALVLYHHINAYEEDGHVVFDIVAYRDNSLYDMFYLKKLDKDFEVNNKLTSIPTCKRFVVPLQYDKDAEVGSNLVKLPTSATAVKEKDGSIYCQPEILCEGIELPRVNYDYNGKKYKYVYATEVQWSPVPTKIAKLNVQTKEVLHWGEDHCWPSEPIFVPSPDAREEDEGVVLTCVVVSEPNKAPFLLILDAKTFKELGRATVNVEMHLDLHGMFIPQNDLGAETE

Target ORF information:

RefSeq Version	XM_015292519.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292519.1

ORF Insert Sequence:

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TACGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCO1 ( XM_015292520.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148006.1 Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X2, mRNA.

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CloneID	OGa41016
Clone ID Related Accession (Same CDS sequence)	XM_015292519.1 , XM_015292520.1
Accession Version	XM_015292520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	beta,beta-carotene 15,15'-monooxygenase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455936.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TACGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148006.1
CDS	269..1849
Translation	METIFNRNKEEHPEPIKAEVQGQLPTWLQGVLLRNGPGMHTIGDTKYNHWFDGLALLHSFTFKNGEVYYRSKYLRSDTYNCNIEANRIVVSEFGTMAYPDPCKNIFAKAFSYLSHTIPEFTDNCLINIMKTGDDYYATSETNFIRKIDPQTLETLDKVDYSKYVAVNLATSHPHYDSAGNILNMGTSIVDKGRTKYVLFKIPSSVPEKEKKKSCFKHLEVVCSIPSRSLLQPSYYHSFGITENYIVFIEQPFKLDIVKLATAYIRGVNWASCLSFHKEDKTWFHFVDRKTKKEVSTKFYTDALVLYHHINAYEEDGHVVFDIVAYRDNSLYDMFYLKKLDKDFEVNNKLTSIPTCKRFVVPLQYDKDAEVGSNLVKLPTSATAVKEKDGSIYCQPEILCEGIELPRVNYDYNGKKYKYVYATEVQWSPVPTKIAKLNVQTKEVLHWGEDHCWPSEPIFVPSPDAREEDEGVVLTCVVVSEPNKAPFLLILDAKTFKELGRATVNVEMHLDLHGMFIPQNDLGAETE

Target ORF information:

RefSeq Version	XM_015292520.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus beta-carotene 15,15'-monooxygenase 1 (BCMO1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015292520.1

ORF Insert Sequence:

ATGGAGACAA TATTTAACAG AAACAAAGAA GAGCATCCAG AGCCCATAAA AGCTGAGGTG 
CAAGGTCAGT TGCCCACTTG GTTGCAAGGG GTACTTCTCC GAAATGGCCC AGGGATGCAC 
ACAATAGGGG ACACTAAATA CAACCACTGG TTTGATGGCT TGGCTCTGCT GCACAGCTTC 
ACGTTTAAAA ATGGTGAAGT TTACTACAGA AGTAAGTACC TCCGAAGTGA CACATACAAC 
TGCAATATAG AAGCAAACCG AATCGTGGTG TCTGAGTTTG GAACCATGGC TTATCCGGAT 
CCATGCAAAA ACATATTTGC CAAGGCATTC TCATACTTAT CTCACACCAT TCCTGAGTTC 
ACGGACAACT GCCTGATCAA CATTATGAAA ACTGGGGATG ATTATTATGC TACCAGTGAG 
ACTAACTTCA TCAGAAAAAT TGATCCACAG ACTCTGGAGA CACTAGATAA GGTAGACTAC 
AGCAAATATG TAGCTGTAAA CTTGGCAACT TCTCACCCAC ACTATGACAG TGCTGGAAAT 
ATTCTCAACA TGGGTACTTC AATTGTTGAT AAAGGGAGAA CAAAATATGT TCTCTTTAAG 
ATCCCTTCCT CTGTACCAGA AAAAGAAAAG AAGAAATCTT GTTTTAAACA CCTGGAAGTA 
GTATGCTCCA TCCCTTCTCG CTCCCTGCTC CAACCAAGCT ACTACCACAG CTTTGGAATC 
ACAGAAAATT ATATTGTGTT CATAGAGCAG CCATTTAAAC TGGATATTGT CAAACTGGCA 
ACTGCCTACA TCCGAGGTGT GAACTGGGCT TCCTGCCTTT CCTTTCATAA GGAGGATAAG 
ACGTGGTTTC ACTTTGTAGA CAGAAAGACG AAAAAAGAAG TATCCACCAA GTTTTACACT 
GATGCTTTGG TGCTTTATCA CCACATAAAT GCTTACGAAG AAGATGGCCA CGTTGTTTTT 
GATATCGTTG CCTACAGAGA CAATAGCTTG TACGATATGT TTTACTTAAA AAAACTGGAC 
AAAGACTTTG AAGTGAACAA CAAGCTTACC TCCATCCCAA CCTGCAAGCG CTTTGTTGTG 
CCTCTGCAGT ATGACAAGGA TGCAGAAGTA GGTTCTAATT TAGTCAAACT TCCAACTTCC 
GCAACTGCTG TAAAAGAAAA AGATGGCAGC ATCTATTGTC AACCTGAAAT ATTATGTGAA 
GGGATAGAAC TGCCTCGTGT CAACTATGAC TACAATGGCA AAAAATACAA GTATGTCTAT 
GCAACAGAAG TCCAGTGGAG CCCAGTTCCT ACAAAGATTG CAAAACTGAA TGTCCAAACA 
AAGGAAGTAC TGCACTGGGG AGAAGACCAC TGCTGGCCCT CAGAGCCCAT CTTTGTTCCC 
AGCCCCGATG CAAGAGAAGA GGATGAAGGT GTTGTTTTGA CCTGTGTTGT GGTGTCTGAG 
CCAAATAAAG CACCCTTCCT ACTCATCTTG GATGCTAAAA CATTCAAAGA ATTGGGCCGA 
GCCACAGTTA ACGTAGAAAT GCATCTGGAC CTGCATGGGA TGTTTATACC ACAGAATGAT 
TTGGGGGCTG AGACGGAATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production.
Experimental cell research358(2)140-146(2017 09)
Praud C,Al Ahmadieh S,Voldoire E,Le Vern Y,Godet E,Courouss? N,Graulet B,Le Bihan Duval E,Berri C,Duclos MJ

Nutrigenetics of carotenoid metabolism in the chicken: a polymorphism at the ?,?-carotene 15,15'-mono-oxygenase 1 (BCMO1) locus affects the response to dietary ?-carotene.
The British journal of nutrition111(12)2079-88(2014 Jun)
Jlali M,Graulet B,Chauveau-Duriot B,Godet E,Praud C,Nunes CS,Le Bihan-Duval E,Berri C,Duclos MJ

A mutation in the promoter of the chicken ?,?-carotene 15,15'-monooxygenase 1 gene alters xanthophyll metabolism through a selective effect on its mRNA abundance in the breast muscle.
Journal of animal science90(12)4280-8(2012 Dec)
Jlali M,Graulet B,Chauveau-Duriot B,Chabault M,Godet E,Leroux S,Praud C,Le Bihan-Duval E,Duclos MJ,Berri C

Detection of a Cis [corrected] eQTL controlling BCMO1 gene expression leads to the identification of a QTG for chicken breast meat color.
PloS one6(7)e14825(2011)
Le Bihan-Duval E,Nadaf J,Berri C,Pitel F,Graulet B,Godet E,Leroux SY,Demeure O,Lagarrigue S,Duby C,Cogburn LA,Beaumont CM,Duclos MJ

Developmental changes in gene expressions of beta-carotene cleavage enzyme and retinoic acid synthesizing enzymes in the chick duodenum.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology148(3)690-7(2007 Nov)
Yamaguchi N,Yamamoto T,Suruga K,Takase S

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BCO1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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