CCD8 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	CCD8
Entrez Gene ID	829417
Full Name	carotenoid cleavage dioxygenase 8
Synonyms	ATCCD8,MAX4,MORE AXILLARY BRANCHING 4,T16I18.20,T16I18_20,carotenoid cleavage dioxygenase 8
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001342177.1	NP_001329787.1	carotenoid cleavage dioxygenase 8
NM_119434.4	NP_195007.2	carotenoid cleavage dioxygenase 8

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCMO1	NP_059125.2
Canis lupus familiaris (dog)	BCMO1	XP_546815.1
Gallus gallus (chicken)	BCMO1	NP_989966.1
Danio rerio (zebrafish)	bcmo1l	NP_956902.1
Arabidopsis thaliana (thale cress)	CCD8	NP_195007.2
Oryza sativa (rice)	Os01g0746400	NP_001044229.2
Magnaporthe oryzae (rice blast fungus)	MGG_08016	XP_003714968.1
Bos taurus (cattle)	BCMO1	NP_001019730.1
Mus musculus (house mouse)	Bcmo1	NP_067461.2
Rattus norvegicus (Norway rat)	Bcmo1	NP_446100.2
Caenorhabditis elegans (roundworm)	bcmo-1	NP_496729.2
Caenorhabditis elegans (roundworm)	bcmo-2	NP_494694.2
Neurospora crassa	NCU11424	XP_001727958.1
Pan troglodytes (chimpanzee)	BCMO1	XP_523435.3
Xenopus tropicalis (tropical clawed frog)	bcmo1	XP_002938186.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Mayer K, Sch?ller C, Wambutt R, Murphy G, Volckaert G, Pohl T, D?sterh?ft A, Stiekema W, Entian KD, Terryn N, Harris B, Ansorge W, Brandt P, Grivell L, Rieger M, Weichselgartner M, de Simone V, Obermaier B, Mache R, M?ller M, Kreis M, Delseny M, Puigdomenech P, Watson M, Schmidtheini T, Reichert B, Portatelle D, Perez-Alonso M, Boutry M, Bancroft I, Vos P, Hoheisel J, Zimmermann W, Wedler H, Ridley P, Langham SA, McCullagh B, Bilham L, Robben J, Van der Schueren J, Grymonprez B, Chuang YJ, Vandenbussche F, Braeken M, Weltjens I, Voet M, Bastiaens I, Aert R, Defoor E, Weitzenegger T, Bothe G, Ramsperger U, Hilbert H, Braun M, Holzer E, Brandt A, Peters S, van Staveren M, Dirske W, Mooijman P, Klein Lankhorst R, Rose M, Hauf J, K?tter P, Berneiser S, Hempel S, Feldpausch M, Lamberth S, Van den Daele H, De Keyser A, Buysshaert C, Gielen J, Villarroel R, De Clercq R, Van Montagu M, Rogers J, Cronin A, Quail M, Bray-Allen S, Clark L, Doggett J, Hall S, Kay M, Lennard N, McLay K, Mayes R, Pettett A, Rajandream MA, Lyne M, Benes V, Rechmann S, Borkova D, Bl?cker H, Scharfe M, Grimm M, L?hnert TH, Dose S, de Haan M, Maarse A, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, Fartmann B, Granderath K, Dauner D, Herzl A, Neumann S, Argiriou A, Vitale D, Liguori R, Piravandi E, Massenet O, Quigley F, Clabauld G, M?ndlein A, Felber R, Schnabl S, Hiller R, Schmidt W, Lecharny A, Aubourg S, Chefdor F, Cooke R, Berger C, Montfort A, Casacuberta E, Gibbons T, Weber N, Vandenbol M, Bargues M, Terol J, Torres A, Perez-Perez A, Purnelle B, Bent E, Johnson S, Tacon D, Jesse T, Heijnen L, Schwarz S, Scholler P, Heber S, Francs P, Bielke C, Frishman D, Haase D, Lemcke K, Mewes HW, Stocker S, Zaccaria P, Bevan M, Wilson RK, de la Bastide M, Habermann K, Parnell L, Dedhia N, Gnoj L, Schutz K, Huang E, Spiegel L, Sehkon M, Murray J, Sheet P, Cordes M, Abu-Threideh J, Stoneking T, Kalicki J, Graves T, Harmon G, Edwards J, Latreille P, Courtney L, Cloud J, Abbott A, Scott K, Johnson D, Minx P, Bentley D, Fulton B, Miller N, Greco T, Kemp K, Kramer J, Fulton L, Mardis E, Dante M, Pepin K, Hillier L, Nelson J, Spieth J, Ryan E, Andrews S, Geisel C, Layman D, Du H, Ali J, Berghoff A, Jones K, Drone K, Cotton M, Joshu C, Antonoiu B, Zidanic M, Strong C, Sun H, Lamar B, Yordan C, Ma P, Zhong J, Preston R, Vil D, Shekher M, Matero A, Shah R, Swaby IK, O'Shaughnessy A, Rodriguez M, Hoffmann J, Till S, Granat S, Shohdy N, Hasegawa A, Hameed A, Lodhi M, Johnson A, Chen E, Marra M, Martienssen R, McCombie WR
Nature402(6763)769-77(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The strigolactone biosynthesis genes MAX3 and MAX4 were drastically induced during dark incubation and treatment with the senescence-promoting phytohormone ethylene, suggesting that strigolactone is synthesized in the leaf during leaf senescence.
Title: Strigolactone Regulates Leaf Senescence in Concert with Ethylene in Arabidopsis.

Wild-type and strigolactone mutants of A. thaliana Atccd7 and Atccd8 induced similar levels of P. ramosa seed germination, suggesting that compounds other than strigolactone function as germination stimulants for P. ramosa in other Brassicaceae spp.
Title: Germination stimulants of Phelipanche ramosa in the rhizosphere of Brassica napus are derived from the glucosinolate pathway.

Results show that MAX3 and MAX4 genes were down-regulated in igi1 (Inflorescence Growth Inhibitor 1) mutants.
Title: Over-expression of the IGI1 leading to altered shoot-branching development related to MAX pathway in Arabidopsis.

activities in vitro using naturally accumulated carotenoids and synthetic apocarotenoid substrates
Title: Carotenoid oxygenases involved in plant branching catalyse a highly specific conserved apocarotenoid cleavage reaction.

the sequential cleavages of beta-carotene by AtCCD7 and AtCCD8 may be initial steps in the synthesis of a carotenoid-derived signaling molecule necessary for the regulation lateral branching. [AtCCD8]
Title: The biochemical characterization of two carotenoid cleavage enzymes from Arabidopsis indicates that a carotenoid-derived compound inhibits lateral branching.

The following CCD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CCD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb16997	NM_119434.4 Latest version!	Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OAb39956	NM_001342177.1 Latest version!	Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CCD8 ( NM_119434.4 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_195007.2 Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.

Price & Availability↑

CloneID	OAb16997
Clone ID Related Accession (Same CDS sequence)	NM_119434.4
Accession Version	NM_119434.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	carotenoid cleavage dioxygenase 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075). On Sep 12, 2016 this sequence version replaced NM_119434.3.

ATGGCTTCTT TGATCACAAC CAAAGCAATG ATGAGTCATC ATCATGTTTT GTCGTCAACT 
AGAATCACTA CTCTTTATTC CGACAATTCC ATCGGCGATC AACAAATAAA AACAAAACCT 
CAAGTCCCTC ACCGGTTATT TGCTCGGAGG ATCTTCGGTG TAACCAGAGC TGTAATTAAT 
TCAGCGGCAC CGTCTCCGTT GCCGGAGAAA GAGAAGGTGG AAGGTGAGAG ACGGTGTCAT 
GTTGCGTGGA CAAGTGTACA ACAAGAGAAT TGGGAGGGTG AACTTACTGT CCAAGGAAAG 
ATACCCACTT GGCTGAATGG TACGTACCTA AGAAACGGTC CTGGTCTATG GAACATTGGA 
GACCACGATT TCCGGCATCT CTTCGACGGC TACTCCACAC TCGTCAAGCT TCAATTCGAT 
GGCGGTCGTA TATTCGCCGC CCACCGTCTC CTTGAATCCG ACGCTTACAA AGCCGCCAAG 
AAACACAATA GGCTTTGTTA CCGTGAATTC TCCGAGACTC CAAAATCGGT GATCATAAAC 
AAAAACCCTT TCTCCGGGAT CGGAGAAATC GTCAGGCTTT TCTCCGGAGA GTCTTTAACG 
GACAACGCCA ACACCGGAGT GATCAAACTC GGTGACGGGC GGGTCATGTG TCTGACGGAG 
ACTCAAAAAG GATCGATTTT AGTCGACCAT GAGACGCTAG AGACGATCGG GAAATTTGAG 
TACGACGACG TATTGTCCGA TCATATGATC CAATCAGCGC ATCCGATAGT GACGGAGACG 
GAGATGTGGA CGTTGATACC GGATTTGGTT AAACCGGGTT ATCGGGTCGT GAGGATGGAA 
GCCGGGTCGA ATAAAAGAGA GGTTGTGGGG CGGGTGAGGT GTCGAAGTGG GTCGTGGGGA 
CCCGGTTGGG TCCATTCGTT TGCGGTGACG GAGAATTATG TTGTAATACC GGAAATGCCC 
CTGAGATATT CGGTGAAGAA TCTTCTTAGA GCTGAGCCGA CGCCACTTTA CAAGTTCGAG 
TGGTGTCCCC AAGACGGAGC TTTTATTCAT GTCATGTCCA AACTCACCGG AGAAGTCGTG 
GCTAGCGTGG AGGTTCCAGC ATACGTAACG TTTCACTTCA TAAACGCGTA TGAAGAAGAT 
AAAAATGGCG ATGGAAAAGC GACGGTCATC ATTGCAGATT GTTGTGAACA CAACGCCGAT 
ACTCGGATAC TCGATATGCT CCGTCTCGAT ACCCTACGTT CTTCCCATGG TCACGACGTT 
TTACCCGATG CTAGGATCGG GAGATTCAGG ATACCATTGG ACGGGAGCAA ATACGGGAAA 
CTAGAGACAG CCGTGGAGGC AGAGAAGCAT GGGAGAGCGA TGGATATGTG CAGCATCAAT 
CCTTTGTATT TGGGTCAAAA ATACCGTTAC GTTTATGCAT GCGGTGCTCA ACGACCTTGT 
AACTTCCCCA ATGCTCTCTC CAAGGTTGAT ATTGTGGAGA AGAAAGTGAA GAACTGGCAC 
GAGCATGGTA TGATACCATC TGAACCATTC TTCGTGCCTC GACCCGGTGC AACCCATGAG 
GATGATGGAG TGGTGATATC GATAGTAAGT GAAGAAAATG GAGGAAGCTT TGCAATCTTG 
CTTGATGGGA GCTCCTTTGA AGAAATAGCA AGAGCCAAGT TTCCCTATGG CCTTCCTTAT 
GGCTTGCATG GTTGCTGGAT CCCCAAAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_195007.2
CDS	121..1833
Translation	MASLITTKAMMSHHHVLSSTRITTLYSDNSIGDQQIKTKPQVPHRLFARRIFGVTRAVINSAAPSPLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCNFPNALSKVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPKD

Target ORF information:

RefSeq Version	NM_119434.4
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_119434.4

ORF Insert Sequence:

ATGGCTTCTT TGATCACAAC CAAAGCAATG ATGAGTCATC ATCATGTTTT GTCGTCAACT 
AGAATCACTA CTCTTTATTC CGACAATTCC ATCGGCGATC AACAAATAAA AACAAAACCT 
CAAGTCCCTC ACCGGTTATT TGCTCGGAGG ATCTTCGGTG TAACCAGAGC TGTAATTAAT 
TCAGCGGCAC CGTCTCCGTT GCCGGAGAAA GAGAAGGTGG AAGGTGAGAG ACGGTGTCAT 
GTTGCGTGGA CAAGTGTACA ACAAGAGAAT TGGGAGGGTG AACTTACTGT CCAAGGAAAG 
ATACCCACTT GGCTGAATGG TACGTACCTA AGAAACGGTC CTGGTCTATG GAACATTGGA 
GACCACGATT TCCGGCATCT CTTCGACGGC TACTCCACAC TCGTCAAGCT TCAATTCGAT 
GGCGGTCGTA TATTCGCCGC CCACCGTCTC CTTGAATCCG ACGCTTACAA AGCCGCCAAG 
AAACACAATA GGCTTTGTTA CCGTGAATTC TCCGAGACTC CAAAATCGGT GATCATAAAC 
AAAAACCCTT TCTCCGGGAT CGGAGAAATC GTCAGGCTTT TCTCCGGAGA GTCTTTAACG 
GACAACGCCA ACACCGGAGT GATCAAACTC GGTGACGGGC GGGTCATGTG TCTGACGGAG 
ACTCAAAAAG GATCGATTTT AGTCGACCAT GAGACGCTAG AGACGATCGG GAAATTTGAG 
TACGACGACG TATTGTCCGA TCATATGATC CAATCAGCGC ATCCGATAGT GACGGAGACG 
GAGATGTGGA CGTTGATACC GGATTTGGTT AAACCGGGTT ATCGGGTCGT GAGGATGGAA 
GCCGGGTCGA ATAAAAGAGA GGTTGTGGGG CGGGTGAGGT GTCGAAGTGG GTCGTGGGGA 
CCCGGTTGGG TCCATTCGTT TGCGGTGACG GAGAATTATG TTGTAATACC GGAAATGCCC 
CTGAGATATT CGGTGAAGAA TCTTCTTAGA GCTGAGCCGA CGCCACTTTA CAAGTTCGAG 
TGGTGTCCCC AAGACGGAGC TTTTATTCAT GTCATGTCCA AACTCACCGG AGAAGTCGTG 
GCTAGCGTGG AGGTTCCAGC ATACGTAACG TTTCACTTCA TAAACGCGTA TGAAGAAGAT 
AAAAATGGCG ATGGAAAAGC GACGGTCATC ATTGCAGATT GTTGTGAACA CAACGCCGAT 
ACTCGGATAC TCGATATGCT CCGTCTCGAT ACCCTACGTT CTTCCCATGG TCACGACGTT 
TTACCCGATG CTAGGATCGG GAGATTCAGG ATACCATTGG ACGGGAGCAA ATACGGGAAA 
CTAGAGACAG CCGTGGAGGC AGAGAAGCAT GGGAGAGCGA TGGATATGTG CAGCATCAAT 
CCTTTGTATT TGGGTCAAAA ATACCGTTAC GTTTATGCAT GCGGTGCTCA ACGACCTTGT 
AACTTCCCCA ATGCTCTCTC CAAGGTTGAT ATTGTGGAGA AGAAAGTGAA GAACTGGCAC 
GAGCATGGTA TGATACCATC TGAACCATTC TTCGTGCCTC GACCCGGTGC AACCCATGAG 
GATGATGGAG TGGTGATATC GATAGTAAGT GAAGAAAATG GAGGAAGCTT TGCAATCTTG 
CTTGATGGGA GCTCCTTTGA AGAAATAGCA AGAGCCAAGT TTCCCTATGG CCTTCCTTAT 
GGCTTGCATG GTTGCTGGAT CCCCAAAGAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CCD8 ( NM_001342177.1 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_001329787.1 Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.

Price & Availability↑

CloneID	OAb39956
Clone ID Related Accession (Same CDS sequence)	NM_001342177.1
Accession Version	NM_001342177.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	carotenoid cleavage dioxygenase 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003075).

ATGGCTTCTT TGATCACAAC CAAAGCAATG ATGAGTCATC ATCATGTTTT GTCGTCAACT 
AGAATCACTA CTCTTTATTC CGACAATTCC ATCGGCGATC AACAAATAAA AACAAAACCT 
CAAGTCCCTC ACCGGTTATT TGCTCGGAGG ATCTTCGGTG TAACCAGAGC TGTAATTAAT 
TCAGCGGCAC CGTCTCCGTT GCCGGAGAAA GAGAAGGTGG AAGGTGAGAG ACGGTGTCAT 
GTTGCGTGGA CAAGTGTACA ACAAGAGAAT TGGGAGGGTG AACTTACTGT CCAAGGAAAG 
ATACCCACTT GGCTGAATGG TACGTACCTA AGAAACGGTC CTGGTCTATG GAACATTGGA 
GACCACGATT TCCGGCATCT CTTCGACGGC TACTCCACAC TCGTCAAGCT TCAATTCGAT 
GGCGGTCGTA TATTCGCCGC CCACCGTCTC CTTGAATCCG ACGCTTACAA AGCCGCCAAG 
AAACACAATA GGCTTTGTTA CCGTGAATTC TCCGAGACTC CAAAATCGGT GATCATAAAC 
AAAAACCCTT TCTCCGGGAT CGGAGAAATC GTCAGGCTTT TCTCCGGAGA GTCTTTAACG 
GACAACGCCA ACACCGGAGT GATCAAACTC GGTGACGGGC GGGTCATGTG TCTGACGGAG 
ACTCAAAAAG GATCGATTTT AGTCGACCAT GAGACGCTAG AGACGATCGG GAAATTTGAG 
TACGACGACG TATTGTCCGA TCATATGATC CAATCAGCGC ATCCGATAGT GACGGAGACG 
GAGATGTGGA CGTTGATACC GGATTTGGTT AAACCGGGTT ATCGGGTCGT GAGGATGGAA 
GCCGGGTCGA ATAAAAGAGA GGTTGTGGGG CGGGTGAGGT GTCGAAGTGG GTCGTGGGGA 
CCCGGTTGGG TCCATTCGTT TGCGGTGACG GAGAATTATG TTGTAATACC GGAAATGCCC 
CTGAGATATT CGGTGAAGAA TCTTCTTAGA GCTGAGCCGA CGCCACTTTA CAAGTTCGAG 
TGGTGTCCCC AAGACGGAGC TTTTATTCAT GTCATGTCCA AACTCACCGG AGAAGTCGTG 
GCTAGCGTGG AGGTTCCAGC ATACGTAACG TTTCACTTCA TAAACGCGTA TGAAGAAGAT 
AAAAATGGCG ATGGAAAAGC GACGGTCATC ATTGCAGATT GTTGTGAACA CAACGCCGAT 
ACTCGGATAC TCGATATGCT CCGTCTCGAT ACCCTACGTT CTTCCCATGG TCACGACGTT 
TTACCCGATG CTAGGATCGG GAGATTCAGG ATACCATTGG ACGGGAGCAA ATACGGGAAA 
CTAGAGACAG CCGTGGAGGC AGAGAAGCAT GGGAGAGCGA TGGATATGTG CAGCATCAAT 
CCTTTGTATT TGGGTCAAAA ATACCGTTAC GTTTATGCAT GCGGTGCTCA ACGACCTTGT 
AACTTCCCCA ATGCTCTCTC CAAGGTAACT TACATACCCC AAACTATCGG TTTCCAATAT 
TCAATCGTTT TGAATGAACC TTTTGATAAT TGTATGAGAC AGGTTGATAT TGTGGAGAAG 
AAAGTGAAGA ACTGGCACGA GCATGGTATG ATACCATCTG AACCATTCTT CGTGCCTCGA 
CCCGGTGCAA CCCATGAGGA TGATGGAGTG GTGATATCGA TAGTAAGTGA AGAAAATGGA 
GGAAGCTTTG CAATCTTGCT TGATGGGAGC TCCTTTGAAG AAATAGCAAG AGCCAAGTTT 
CCCTATGGCC TTCCTTATGG CTTGCATGGT TGCTGGATCC CCAAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001329787.1
CDS	121..1911
Translation	MASLITTKAMMSHHHVLSSTRITTLYSDNSIGDQQIKTKPQVPHRLFARRIFGVTRAVINSAAPSPLPEKEKVEGERRCHVAWTSVQQENWEGELTVQGKIPTWLNGTYLRNGPGLWNIGDHDFRHLFDGYSTLVKLQFDGGRIFAAHRLLESDAYKAAKKHNRLCYREFSETPKSVIINKNPFSGIGEIVRLFSGESLTDNANTGVIKLGDGRVMCLTETQKGSILVDHETLETIGKFEYDDVLSDHMIQSAHPIVTETEMWTLIPDLVKPGYRVVRMEAGSNKREVVGRVRCRSGSWGPGWVHSFAVTENYVVIPEMPLRYSVKNLLRAEPTPLYKFEWCPQDGAFIHVMSKLTGEVVASVEVPAYVTFHFINAYEEDKNGDGKATVIIADCCEHNADTRILDMLRLDTLRSSHGHDVLPDARIGRFRIPLDGSKYGKLETAVEAEKHGRAMDMCSINPLYLGQKYRYVYACGAQRPCNFPNALSKVTYIPQTIGFQYSIVLNEPFDNCMRQVDIVEKKVKNWHEHGMIPSEPFFVPRPGATHEDDGVVISIVSEENGGSFAILLDGSSFEEIARAKFPYGLPYGLHGCWIPKD

Target ORF information:

RefSeq Version	NM_001342177.1
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana carotenoid cleavage dioxygenase 8 (CCD8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001342177.1

ORF Insert Sequence:

ATGGCTTCTT TGATCACAAC CAAAGCAATG ATGAGTCATC ATCATGTTTT GTCGTCAACT 
AGAATCACTA CTCTTTATTC CGACAATTCC ATCGGCGATC AACAAATAAA AACAAAACCT 
CAAGTCCCTC ACCGGTTATT TGCTCGGAGG ATCTTCGGTG TAACCAGAGC TGTAATTAAT 
TCAGCGGCAC CGTCTCCGTT GCCGGAGAAA GAGAAGGTGG AAGGTGAGAG ACGGTGTCAT 
GTTGCGTGGA CAAGTGTACA ACAAGAGAAT TGGGAGGGTG AACTTACTGT CCAAGGAAAG 
ATACCCACTT GGCTGAATGG TACGTACCTA AGAAACGGTC CTGGTCTATG GAACATTGGA 
GACCACGATT TCCGGCATCT CTTCGACGGC TACTCCACAC TCGTCAAGCT TCAATTCGAT 
GGCGGTCGTA TATTCGCCGC CCACCGTCTC CTTGAATCCG ACGCTTACAA AGCCGCCAAG 
AAACACAATA GGCTTTGTTA CCGTGAATTC TCCGAGACTC CAAAATCGGT GATCATAAAC 
AAAAACCCTT TCTCCGGGAT CGGAGAAATC GTCAGGCTTT TCTCCGGAGA GTCTTTAACG 
GACAACGCCA ACACCGGAGT GATCAAACTC GGTGACGGGC GGGTCATGTG TCTGACGGAG 
ACTCAAAAAG GATCGATTTT AGTCGACCAT GAGACGCTAG AGACGATCGG GAAATTTGAG 
TACGACGACG TATTGTCCGA TCATATGATC CAATCAGCGC ATCCGATAGT GACGGAGACG 
GAGATGTGGA CGTTGATACC GGATTTGGTT AAACCGGGTT ATCGGGTCGT GAGGATGGAA 
GCCGGGTCGA ATAAAAGAGA GGTTGTGGGG CGGGTGAGGT GTCGAAGTGG GTCGTGGGGA 
CCCGGTTGGG TCCATTCGTT TGCGGTGACG GAGAATTATG TTGTAATACC GGAAATGCCC 
CTGAGATATT CGGTGAAGAA TCTTCTTAGA GCTGAGCCGA CGCCACTTTA CAAGTTCGAG 
TGGTGTCCCC AAGACGGAGC TTTTATTCAT GTCATGTCCA AACTCACCGG AGAAGTCGTG 
GCTAGCGTGG AGGTTCCAGC ATACGTAACG TTTCACTTCA TAAACGCGTA TGAAGAAGAT 
AAAAATGGCG ATGGAAAAGC GACGGTCATC ATTGCAGATT GTTGTGAACA CAACGCCGAT 
ACTCGGATAC TCGATATGCT CCGTCTCGAT ACCCTACGTT CTTCCCATGG TCACGACGTT 
TTACCCGATG CTAGGATCGG GAGATTCAGG ATACCATTGG ACGGGAGCAA ATACGGGAAA 
CTAGAGACAG CCGTGGAGGC AGAGAAGCAT GGGAGAGCGA TGGATATGTG CAGCATCAAT 
CCTTTGTATT TGGGTCAAAA ATACCGTTAC GTTTATGCAT GCGGTGCTCA ACGACCTTGT 
AACTTCCCCA ATGCTCTCTC CAAGGTAACT TACATACCCC AAACTATCGG TTTCCAATAT 
TCAATCGTTT TGAATGAACC TTTTGATAAT TGTATGAGAC AGGTTGATAT TGTGGAGAAG 
AAAGTGAAGA ACTGGCACGA GCATGGTATG ATACCATCTG AACCATTCTT CGTGCCTCGA 
CCCGGTGCAA CCCATGAGGA TGATGGAGTG GTGATATCGA TAGTAAGTGA AGAAAATGGA 
GGAAGCTTTG CAATCTTGCT TGATGGGAGC TCCTTTGAAG AAATAGCAAG AGCCAAGTTT 
CCCTATGGCC TTCCTTATGG CTTGCATGGT TGCTGGATCC CCAAAGATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature402(6763)769-77(1999 Dec)
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CCD8 cDNA ORF clone, Arabidopsis thaliana(thale cress)

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