gRNAs
The following APP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OGa26020 | |
| Clone ID Related Accession (Same CDS sequence) | NM_204308.1 | |
| Accession Version | NM_204308.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2256bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-20 | |
| Organism | Gallus gallus(chicken) | |
| Product | amyloid-beta A4 protein precursor | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF289219.1. ##Evidence-Data-START## Transcript exon combination :: AF289219.1 [ECO:0000332] ##Evidence-Data-END## | |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_989639.1 |
| CDS | 109..2364 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_204308.1 |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa30230 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015299094.1 , XM_015299094.2 | |
| Accession Version | XM_015299094.1 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-01-03 | |
| Organism | Gallus gallus(chicken) | |
| Product | amyloid beta A4 protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455735.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015154580.1 |
| CDS | 155..2242 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015299094.1 |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OGa30230 | |
| Clone ID Related Accession (Same CDS sequence) | XM_015299094.1 , XM_015299094.2 | |
| Accession Version | XM_015299094.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-16 | |
| Organism | Gallus gallus(chicken) | |
| Product | amyloid-beta A4 protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015299094.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_015154580.1 |
| CDS | 151..2238 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_015299094.2 |
1 | ATGCTGCCCC ACTTAGCCCT TTTGCTGCTG GCCGCCGGCG CCGCCCGGGC CCTTGAGGTG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Inhibition of choline acetyltransferase as a mechanism for cholinergic dysfunction induced by amyloid-? peptide oligomers. |
Comparison of pharmacological modulation of APP metabolism in primary chicken telencephalic neurons and in a human neuroglioma cell line. |
Hen lysozyme amyloid fibrils induce aggregation of erythrocytes and lipid vesicles. |
The chick embryo appears as a natural model for research in beta-amyloid precursor protein processing. |
Presence of a "CAGA box" in the APP gene unique to amyloid plaque-forming species and absent in all APLP-1/2 genes: implications in Alzheimer's disease. |
