KIAA1257 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015293468.1	XP_015148954.1	uncharacterized protein KIAA1257 homolog isoform X4
XM_025154698.1	XP_025010466.1	uncharacterized protein KIAA1257 homolog isoform X6
XM_004944519.2	XP_004944576.2	uncharacterized protein KIAA1257 homolog isoform X1
XM_015293466.1	XP_015148952.1	uncharacterized protein KIAA1257 homolog isoform X2
XM_015293469.1	XP_015148955.1	uncharacterized protein KIAA1257 homolog isoform X5
XM_004944519.3	XP_004944576.2	uncharacterized protein KIAA1257 homolog isoform X1
XM_015293467.2	XP_015148953.1	uncharacterized protein KIAA1257 homolog isoform X3
XM_015293468.2	XP_015148954.1	uncharacterized protein KIAA1257 homolog isoform X4
XM_015293469.2	XP_015148955.1	uncharacterized protein KIAA1257 homolog isoform X5
XM_025154699.1	XP_025010467.1	uncharacterized protein KIAA1257 homolog isoform X7
XM_015293467.1	XP_015148953.1	uncharacterized protein KIAA1257 homolog isoform X3
XM_004944522.2	XP_004944579.2	uncharacterized protein KIAA1257 homolog isoform X6
XM_015293466.2	XP_015148952.1	uncharacterized protein KIAA1257 homolog isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa144057	XM_025154699.1 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa41709	XM_004944519.3 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41710	XM_015293466.2 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41711	XM_015293467.2 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41712	XM_015293468.2 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa41713	XM_015293469.2 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa41714	XM_025154698.1 Latest version!	Gallus gallus KIAA1257 (KIAA1257), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa41714	XM_004944522.2 Latest version!	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OGa41709	XM_004944519.2	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41712	XM_015293468.1	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa41713	XM_015293469.1	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OGa41711	XM_015293467.1	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OGa41710	XM_015293466.1	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

KIAA1257 ( XM_025154699.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010467.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OGa144057
Clone ID Related Accession (Same CDS sequence)	XM_025154699.1
Accession Version	XM_025154699.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3243bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG ATTTGAGGGT 
AACGCCCAAC TTCTGAAACA GAAAGCAGTT GCTGCTCCTT CACATAATGC AGCTTCAGAA 
GAAACTGGGG AAACAGCCAG CAATGTGGGA ATTCCGATGT GTCAAACAGA CAAGACAGCT 
ACCTTTAATG GTAGAAGAGA TTCTGGTGCT AAAAAAGGCA TGGATACTAC TAGGGCAAAC 
ATTGCATCAC TGCAGTTGGA TCTCATGCCT CTCCTCTCAG GAGAAAATTC AGTGATCAGT 
CGACTAGAAG AAAACAGTCC TATTCTTTTA GATGCTTATG TGTCTTTCAC TGTAGAAACG 
CCTCTTCTGT CTGAAAGACA AATACGTGAA TTGAATCCAC TGGTTATAAA GATCTGTTCA 
GCTACCAGCC TCCCGAACAC ACCTATACCA ATAGAAGTGC TACAGAAATT GTGTGTTCCT 
ACCTACTGCA AATACAAATT CCACAATTTT CCAGCTCATC AGACTCATGG ACAAATCCAC 
GGAACTCATG TCTACTTTAA GGATGTTAAT GTATTTCTAA CAGGAAGAAT GAAACCTGGG 
GAGTTACAGA GGTGTCTTAG AGGTCCCCCT TTGGAGATTG AAGTTCATGA TCGAGATAGA 
AAGATTGAAG ACAACAAAAA AGAGCCATGT CTATTTGGGG AGGATCACAC TGATGAAAAT 
CTAGGAGAGT TGAGACCCCT CCCTTGCCAG TCTGCAATCT GTAATTCTTT TGCAGAGGAT 
GAAGTGTGGC ATCCACATGG GGTAGCTAAA GTAAGTCTAA GTGATCTATT GTTGGGTAAA 
AAGTACTTGA CTGTTAGTGC ACCCATTCGT AGCTGTTCAA CTCCAAATAC AGCTGCTTTC 
AATAAAAAGA ATAAAAGAAA GAAGATAGGG AAGAAGCATC CACAGATCCT ATTACCCATG 
GGACATTATC TACAATCAGA CTCGCTTTTG AAATTAAGAG TGGAAATAGC AGTGCCATTG 
AGAATGCATG CAGAAATTGA TGATGCTCAA GTGACGGATT GCCCGTACAG TTGTATAGTC 
TACATTTTTG ACTACAGTAA TTCATTTTTA TTACATGATT TGGTGGAAGA GATTACAGAA 
ATCAATGCGA AAGCCCTTCA GCTGGACTGT TATCCTCCAC ACTTGATCAG AACATCTCTT 
GCTACATTAA AACTAAAAAG CATGCTTGAG AAGGTTTCTG AGTTAGATGT TGTTACTGGC 
TTTCACTTAC TGGATGGAGC TACTCACCTC TTTGTCTTGG AGGGTTTAAA AGACAAAGCC 
ATCAAGAGAC TCTGGGATAG GCACTTTGAA AGAACTTACA GACATGAAGA TGGACAACTG 
GAAATACTAT ATAATTCACA ACTGTCTTTC AGTCAGCGCC TGTATACCGA CCTGGAAGCA 
ATCTTTTATC GTTTCCACCT CTGTAAGCCC CTCTTCACTA TACTGAAACA GCCTCTGCTT 
TATGTCAGGG GAGCAGTTCC TTGGGCGTGT TTCCAAGCCT TGTCCAGGCT CAGTTACCTC 
TGTCACAGTA AGAGGTTGAG GGATGTCATT CAGGGGGATC TGCTGCCCTC AGCAGAAATG 
ATCACAGCGT TGAGTCGAGA GTATGGAGTT CCATTAGCTG ATGAGGATCT CTTCACTCAG 
AAACCTCCTC TACTCTTATT TTCGTCTGAT TATACACCAC CAGGAAATGT AAGCAGAGAG 
AAAAAGGCAC GATATTCTTC ATTAGATCAC TACAACGAAA GATATGTGCT GTGGAAGAAA 
GAAATAGCAG ACAAAAAGTC TTTTGAGAGA AACCATATTA GGGCTAACAT TGATGCTGTG 
CGCCAGCTTA AAGGAAAGAT GAAAAGGCTG AAGTTTGAAA CTTTCAGGAT TTCTCCTGCC 
GATGGCAAAT CTGTTTATAA CTATAGCTCT CAGCGCCTGA ATTCTGCAGA ACTTGCAAAG 
AAGCATCTTC GCCAGGAAAT GGCAAAGGAT CCAAAAACAA GGTTCAGTTA TTGTCACGAG 
TATCTTTCAG CCACGTTTGA CCCAGTTGAT GTGGTTGCTG CCTGTAAGGA GTCCTTTGCA 
AAATCCAAGA GTACGTGGCT GTCACCAGAT GGATTTGTAT TTGCTGGATA TAAGAGCAGC 
ATTGAAAGCA ACCTGCACCC TCGGATGCCT GATGAGGCAC GTGTGGAGGA GTTACGAGAG 
AAATGGCAGG AGAATGCTCT GTTTGCTAAC ATGGCGCCAG TGCTGCCACG AGGCAGATGG 
AGCTGGGACA AACGGCATGT TGACTTCGAG CTCTACAAGA AGCCGCCTGA ACTCCCCATG 
GCAACAGCCA CACTGACAGA TGCTACTCAC TTTGTGCAAA ATGTGTGTGA TGATACCAAG 
CTGAAGGTTC ACTGGTGTTC TGCAGCAGCT GAGTTACGCG CACGGGAGCC AGGAGCCACT 
TCTGAGCTAC AGCATCTCCA AGGACTTCTG AAAGATGAGC CTCTGAAATT CCCACTCAGG 
AAGCCAGGAC TGATTTTGAA GCCTAACCCA GCCCTCTCAG TGTTGGACTC TCAGAACAGC 
TCAGATGTCC TGCCAGGAAA GAAGAGCGTT TGCAGTGGCT TTGTGCCAGG CCTTCAAGAC 
CACCACAGCC GGAAGTGGAA CAAGAACATT ATCCCCTGGC ACAACAGAGA GCATAAGAAG 
ATTGAAAAAC TTGAAGGCGC AGATTTCAAT TTACTTGGCT CTAAACAATC AGTTTACAAG 
AGTGACCAAG CAGCAGGCAG ACACGCTACA ACCTATGCAC AACAGGAAGA CCCTCTCCAG 
AATGATATTC TGGATACAGC TACTGCATCA CAGCGTTGGG ATGACCCCCT CAGTGGACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010467.1
CDS	214..3456
Translation	MPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_025154699.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154699.1

ORF Insert Sequence:

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG ATTTGAGGGT 
AACGCCCAAC TTCTGAAACA GAAAGCAGTT GCTGCTCCTT CACATAATGC AGCTTCAGAA 
GAAACTGGGG AAACAGCCAG CAATGTGGGA ATTCCGATGT GTCAAACAGA CAAGACAGCT 
ACCTTTAATG GTAGAAGAGA TTCTGGTGCT AAAAAAGGCA TGGATACTAC TAGGGCAAAC 
ATTGCATCAC TGCAGTTGGA TCTCATGCCT CTCCTCTCAG GAGAAAATTC AGTGATCAGT 
CGACTAGAAG AAAACAGTCC TATTCTTTTA GATGCTTATG TGTCTTTCAC TGTAGAAACG 
CCTCTTCTGT CTGAAAGACA AATACGTGAA TTGAATCCAC TGGTTATAAA GATCTGTTCA 
GCTACCAGCC TCCCGAACAC ACCTATACCA ATAGAAGTGC TACAGAAATT GTGTGTTCCT 
ACCTACTGCA AATACAAATT CCACAATTTT CCAGCTCATC AGACTCATGG ACAAATCCAC 
GGAACTCATG TCTACTTTAA GGATGTTAAT GTATTTCTAA CAGGAAGAAT GAAACCTGGG 
GAGTTACAGA GGTGTCTTAG AGGTCCCCCT TTGGAGATTG AAGTTCATGA TCGAGATAGA 
AAGATTGAAG ACAACAAAAA AGAGCCATGT CTATTTGGGG AGGATCACAC TGATGAAAAT 
CTAGGAGAGT TGAGACCCCT CCCTTGCCAG TCTGCAATCT GTAATTCTTT TGCAGAGGAT 
GAAGTGTGGC ATCCACATGG GGTAGCTAAA GTAAGTCTAA GTGATCTATT GTTGGGTAAA 
AAGTACTTGA CTGTTAGTGC ACCCATTCGT AGCTGTTCAA CTCCAAATAC AGCTGCTTTC 
AATAAAAAGA ATAAAAGAAA GAAGATAGGG AAGAAGCATC CACAGATCCT ATTACCCATG 
GGACATTATC TACAATCAGA CTCGCTTTTG AAATTAAGAG TGGAAATAGC AGTGCCATTG 
AGAATGCATG CAGAAATTGA TGATGCTCAA GTGACGGATT GCCCGTACAG TTGTATAGTC 
TACATTTTTG ACTACAGTAA TTCATTTTTA TTACATGATT TGGTGGAAGA GATTACAGAA 
ATCAATGCGA AAGCCCTTCA GCTGGACTGT TATCCTCCAC ACTTGATCAG AACATCTCTT 
GCTACATTAA AACTAAAAAG CATGCTTGAG AAGGTTTCTG AGTTAGATGT TGTTACTGGC 
TTTCACTTAC TGGATGGAGC TACTCACCTC TTTGTCTTGG AGGGTTTAAA AGACAAAGCC 
ATCAAGAGAC TCTGGGATAG GCACTTTGAA AGAACTTACA GACATGAAGA TGGACAACTG 
GAAATACTAT ATAATTCACA ACTGTCTTTC AGTCAGCGCC TGTATACCGA CCTGGAAGCA 
ATCTTTTATC GTTTCCACCT CTGTAAGCCC CTCTTCACTA TACTGAAACA GCCTCTGCTT 
TATGTCAGGG GAGCAGTTCC TTGGGCGTGT TTCCAAGCCT TGTCCAGGCT CAGTTACCTC 
TGTCACAGTA AGAGGTTGAG GGATGTCATT CAGGGGGATC TGCTGCCCTC AGCAGAAATG 
ATCACAGCGT TGAGTCGAGA GTATGGAGTT CCATTAGCTG ATGAGGATCT CTTCACTCAG 
AAACCTCCTC TACTCTTATT TTCGTCTGAT TATACACCAC CAGGAAATGT AAGCAGAGAG 
AAAAAGGCAC GATATTCTTC ATTAGATCAC TACAACGAAA GATATGTGCT GTGGAAGAAA 
GAAATAGCAG ACAAAAAGTC TTTTGAGAGA AACCATATTA GGGCTAACAT TGATGCTGTG 
CGCCAGCTTA AAGGAAAGAT GAAAAGGCTG AAGTTTGAAA CTTTCAGGAT TTCTCCTGCC 
GATGGCAAAT CTGTTTATAA CTATAGCTCT CAGCGCCTGA ATTCTGCAGA ACTTGCAAAG 
AAGCATCTTC GCCAGGAAAT GGCAAAGGAT CCAAAAACAA GGTTCAGTTA TTGTCACGAG 
TATCTTTCAG CCACGTTTGA CCCAGTTGAT GTGGTTGCTG CCTGTAAGGA GTCCTTTGCA 
AAATCCAAGA GTACGTGGCT GTCACCAGAT GGATTTGTAT TTGCTGGATA TAAGAGCAGC 
ATTGAAAGCA ACCTGCACCC TCGGATGCCT GATGAGGCAC GTGTGGAGGA GTTACGAGAG 
AAATGGCAGG AGAATGCTCT GTTTGCTAAC ATGGCGCCAG TGCTGCCACG AGGCAGATGG 
AGCTGGGACA AACGGCATGT TGACTTCGAG CTCTACAAGA AGCCGCCTGA ACTCCCCATG 
GCAACAGCCA CACTGACAGA TGCTACTCAC TTTGTGCAAA ATGTGTGTGA TGATACCAAG 
CTGAAGGTTC ACTGGTGTTC TGCAGCAGCT GAGTTACGCG CACGGGAGCC AGGAGCCACT 
TCTGAGCTAC AGCATCTCCA AGGACTTCTG AAAGATGAGC CTCTGAAATT CCCACTCAGG 
AAGCCAGGAC TGATTTTGAA GCCTAACCCA GCCCTCTCAG TGTTGGACTC TCAGAACAGC 
TCAGATGTCC TGCCAGGAAA GAAGAGCGTT TGCAGTGGCT TTGTGCCAGG CCTTCAAGAC 
CACCACAGCC GGAAGTGGAA CAAGAACATT ATCCCCTGGC ACAACAGAGA GCATAAGAAG 
ATTGAAAAAC TTGAAGGCGC AGATTTCAAT TTACTTGGCT CTAAACAATC AGTTTACAAG 
AGTGACCAAG CAGCAGGCAG ACACGCTACA ACCTATGCAC AACAGGAAGA CCCTCTCCAG 
AATGATATTC TGGATACAGC TACTGCATCA CAGCGTTGGG ATGACCCCCT CAGTGGACAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_004944519.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944576.2 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa41709
Clone ID Related Accession (Same CDS sequence)	XM_004944519.2 , XM_004944519.3
Accession Version	XM_004944519.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AATTTACTTG GCTCTAAACA ATCAGTTTAC 
AAGAGTGACC AAGCAGCAGG CAGACACGCT ACAACCTATG CACAACAGGA AGACCCTCTC 
CAGAATGATA TTCTGGATAC AGCTACTGCA TCACAGCGTT GGGATGACCC CCTCAGTGGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944576.2
CDS	58..3663
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_004944519.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944519.2

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AATTTACTTG GCTCTAAACA ATCAGTTTAC 
AAGAGTGACC AAGCAGCAGG CAGACACGCT ACAACCTATG CACAACAGGA AGACCCTCTC 
CAGAATGATA TTCTGGATAC AGCTACTGCA TCACAGCGTT GGGATGACCC CCTCAGTGGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_004944519.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944576.2 Gallus gallus KIAA1257 (KIAA1257), transcript variant X1, mRNA.

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CloneID	OGa41709
Clone ID Related Accession (Same CDS sequence)	XM_004944519.2 , XM_004944519.3
Accession Version	XM_004944519.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004944519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AATTTACTTG GCTCTAAACA ATCAGTTTAC 
AAGAGTGACC AAGCAGCAGG CAGACACGCT ACAACCTATG CACAACAGGA AGACCCTCTC 
CAGAATGATA TTCTGGATAC AGCTACTGCA TCACAGCGTT GGGATGACCC CCTCAGTGGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944576.2
CDS	1438..5043
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_004944519.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944519.3

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AATTTACTTG GCTCTAAACA ATCAGTTTAC 
AAGAGTGACC AAGCAGCAGG CAGACACGCT ACAACCTATG CACAACAGGA AGACCCTCTC 
CAGAATGATA TTCTGGATAC AGCTACTGCA TCACAGCGTT GGGATGACCC CCTCAGTGGA 
CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293466.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148952.1 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X2, mRNA.

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CloneID	OGa41710
Clone ID Related Accession (Same CDS sequence)	XM_015293466.1 , XM_015293466.2
Accession Version	XM_015293466.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGATT TGAGGGTAAC 
GCCCAACTTC TGAAACAGAA AGCAGTTGCT GCTCCTTCAC ATAATGCAGC TTCAGAAGAA 
ACTGGGGAAA CAGCCAGCAA TGTGGGAATT CCGATGTGTC AAACAGACAA GACAGCTACC 
TTTAATGGTA GAAGAGATTC TGGTGCTAAA AAAGGCATGG ATACTACTAG GGCAAACATT 
GCATCACTGC AGTTGGATCT CATGCCTCTC CTCTCAGGAG AAAATTCAGT GATCAGTCGA 
CTAGAAGAAA ACAGTCCTAT TCTTTTAGAT GCTTATGTGT CTTTCACTGT AGAAACGCCT 
CTTCTGTCTG AAAGACAAAT ACGTGAATTG AATCCACTGG TTATAAAGAT CTGTTCAGCT 
ACCAGCCTCC CGAACACACC TATACCAATA GAAGTGCTAC AGAAATTGTG TGTTCCTACC 
TACTGCAAAT ACAAATTCCA CAATTTTCCA GCTCATCAGA CTCATGGACA AATCCACGGA 
ACTCATGTCT ACTTTAAGGA TGTTAATGTA TTTCTAACAG GAAGAATGAA ACCTGGGGAG 
TTACAGAGGT GTCTTAGAGG TCCCCCTTTG GAGATTGAAG TTCATGATCG AGATAGAAAG 
ATTGAAGACA ACAAAAAAGA GCCATGTCTA TTTGGGGAGG ATCACACTGA TGAAAATCTA 
GGAGAGTTGA GACCCCTCCC TTGCCAGTCT GCAATCTGTA ATTCTTTTGC AGAGGATGAA 
GTGTGGCATC CACATGGGGT AGCTAAAGTA AGTCTAAGTG ATCTATTGTT GGGTAAAAAG 
TACTTGACTG TTAGTGCACC CATTCGTAGC TGTTCAACTC CAAATACAGC TGCTTTCAAT 
AAAAAGAATA AAAGAAAGAA GATAGGGAAG AAGCATCCAC AGATCCTATT ACCCATGGGA 
CATTATCTAC AATCAGACTC GCTTTTGAAA TTAAGAGTGG AAATAGCAGT GCCATTGAGA 
ATGCATGCAG AAATTGATGA TGCTCAAGTG ACGGATTGCC CGTACAGTTG TATAGTCTAC 
ATTTTTGACT ACAGTAATTC ATTTTTATTA CATGATTTGG TGGAAGAGAT TACAGAAATC 
AATGCGAAAG CCCTTCAGCT GGACTGTTAT CCTCCACACT TGATCAGAAC ATCTCTTGCT 
ACATTAAAAC TAAAAAGCAT GCTTGAGAAG GTTTCTGAGT TAGATGTTGT TACTGGCTTT 
CACTTACTGG ATGGAGCTAC TCACCTCTTT GTCTTGGAGG GTTTAAAAGA CAAAGCCATC 
AAGAGACTCT GGGATAGGCA CTTTGAAAGA ACTTACAGAC ATGAAGATGG ACAACTGGAA 
ATACTATATA ATTCACAACT GTCTTTCAGT CAGCGCCTGT ATACCGACCT GGAAGCAATC 
TTTTATCGTT TCCACCTCTG TAAGCCCCTC TTCACTATAC TGAAACAGCC TCTGCTTTAT 
GTCAGGGGAG CAGTTCCTTG GGCGTGTTTC CAAGCCTTGT CCAGGCTCAG TTACCTCTGT 
CACAGTAAGA GGTTGAGGGA TGTCATTCAG GGGGATCTGC TGCCCTCAGC AGAAATGATC 
ACAGCGTTGA GTCGAGAGTA TGGAGTTCCA TTAGCTGATG AGGATCTCTT CACTCAGAAA 
CCTCCTCTAC TCTTATTTTC GTCTGATTAT ACACCACCAG GAAATGTAAG CAGAGAGAAA 
AAGGCACGAT ATTCTTCATT AGATCACTAC AACGAAAGAT ATGTGCTGTG GAAGAAAGAA 
ATAGCAGACA AAAAGTCTTT TGAGAGAAAC CATATTAGGG CTAACATTGA TGCTGTGCGC 
CAGCTTAAAG GAAAGATGAA AAGGCTGAAG TTTGAAACTT TCAGGATTTC TCCTGCCGAT 
GGCAAATCTG TTTATAACTA TAGCTCTCAG CGCCTGAATT CTGCAGAACT TGCAAAGAAG 
CATCTTCGCC AGGAAATGGC AAAGGATCCA AAAACAAGGT TCAGTTATTG TCACGAGTAT 
CTTTCAGCCA CGTTTGACCC AGTTGATGTG GTTGCTGCCT GTAAGGAGTC CTTTGCAAAA 
TCCAAGAGTA CGTGGCTGTC ACCAGATGGA TTTGTATTTG CTGGATATAA GAGCAGCATT 
GAAAGCAACC TGCACCCTCG GATGCCTGAT GAGGCACGTG TGGAGGAGTT ACGAGAGAAA 
TGGCAGGAGA ATGCTCTGTT TGCTAACATG GCGCCAGTGC TGCCACGAGG CAGATGGAGC 
TGGGACAAAC GGCATGTTGA CTTCGAGCTC TACAAGAAGC CGCCTGAACT CCCCATGGCA 
ACAGCCACAC TGACAGATGC TACTCACTTT GTGCAAAATG TGTGTGATGA TACCAAGCTG 
AAGGTTCACT GGTGTTCTGC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148952.1
CDS	58..3657
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293466.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293466.1

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGATT TGAGGGTAAC 
GCCCAACTTC TGAAACAGAA AGCAGTTGCT GCTCCTTCAC ATAATGCAGC TTCAGAAGAA 
ACTGGGGAAA CAGCCAGCAA TGTGGGAATT CCGATGTGTC AAACAGACAA GACAGCTACC 
TTTAATGGTA GAAGAGATTC TGGTGCTAAA AAAGGCATGG ATACTACTAG GGCAAACATT 
GCATCACTGC AGTTGGATCT CATGCCTCTC CTCTCAGGAG AAAATTCAGT GATCAGTCGA 
CTAGAAGAAA ACAGTCCTAT TCTTTTAGAT GCTTATGTGT CTTTCACTGT AGAAACGCCT 
CTTCTGTCTG AAAGACAAAT ACGTGAATTG AATCCACTGG TTATAAAGAT CTGTTCAGCT 
ACCAGCCTCC CGAACACACC TATACCAATA GAAGTGCTAC AGAAATTGTG TGTTCCTACC 
TACTGCAAAT ACAAATTCCA CAATTTTCCA GCTCATCAGA CTCATGGACA AATCCACGGA 
ACTCATGTCT ACTTTAAGGA TGTTAATGTA TTTCTAACAG GAAGAATGAA ACCTGGGGAG 
TTACAGAGGT GTCTTAGAGG TCCCCCTTTG GAGATTGAAG TTCATGATCG AGATAGAAAG 
ATTGAAGACA ACAAAAAAGA GCCATGTCTA TTTGGGGAGG ATCACACTGA TGAAAATCTA 
GGAGAGTTGA GACCCCTCCC TTGCCAGTCT GCAATCTGTA ATTCTTTTGC AGAGGATGAA 
GTGTGGCATC CACATGGGGT AGCTAAAGTA AGTCTAAGTG ATCTATTGTT GGGTAAAAAG 
TACTTGACTG TTAGTGCACC CATTCGTAGC TGTTCAACTC CAAATACAGC TGCTTTCAAT 
AAAAAGAATA AAAGAAAGAA GATAGGGAAG AAGCATCCAC AGATCCTATT ACCCATGGGA 
CATTATCTAC AATCAGACTC GCTTTTGAAA TTAAGAGTGG AAATAGCAGT GCCATTGAGA 
ATGCATGCAG AAATTGATGA TGCTCAAGTG ACGGATTGCC CGTACAGTTG TATAGTCTAC 
ATTTTTGACT ACAGTAATTC ATTTTTATTA CATGATTTGG TGGAAGAGAT TACAGAAATC 
AATGCGAAAG CCCTTCAGCT GGACTGTTAT CCTCCACACT TGATCAGAAC ATCTCTTGCT 
ACATTAAAAC TAAAAAGCAT GCTTGAGAAG GTTTCTGAGT TAGATGTTGT TACTGGCTTT 
CACTTACTGG ATGGAGCTAC TCACCTCTTT GTCTTGGAGG GTTTAAAAGA CAAAGCCATC 
AAGAGACTCT GGGATAGGCA CTTTGAAAGA ACTTACAGAC ATGAAGATGG ACAACTGGAA 
ATACTATATA ATTCACAACT GTCTTTCAGT CAGCGCCTGT ATACCGACCT GGAAGCAATC 
TTTTATCGTT TCCACCTCTG TAAGCCCCTC TTCACTATAC TGAAACAGCC TCTGCTTTAT 
GTCAGGGGAG CAGTTCCTTG GGCGTGTTTC CAAGCCTTGT CCAGGCTCAG TTACCTCTGT 
CACAGTAAGA GGTTGAGGGA TGTCATTCAG GGGGATCTGC TGCCCTCAGC AGAAATGATC 
ACAGCGTTGA GTCGAGAGTA TGGAGTTCCA TTAGCTGATG AGGATCTCTT CACTCAGAAA 
CCTCCTCTAC TCTTATTTTC GTCTGATTAT ACACCACCAG GAAATGTAAG CAGAGAGAAA 
AAGGCACGAT ATTCTTCATT AGATCACTAC AACGAAAGAT ATGTGCTGTG GAAGAAAGAA 
ATAGCAGACA AAAAGTCTTT TGAGAGAAAC CATATTAGGG CTAACATTGA TGCTGTGCGC 
CAGCTTAAAG GAAAGATGAA AAGGCTGAAG TTTGAAACTT TCAGGATTTC TCCTGCCGAT 
GGCAAATCTG TTTATAACTA TAGCTCTCAG CGCCTGAATT CTGCAGAACT TGCAAAGAAG 
CATCTTCGCC AGGAAATGGC AAAGGATCCA AAAACAAGGT TCAGTTATTG TCACGAGTAT 
CTTTCAGCCA CGTTTGACCC AGTTGATGTG GTTGCTGCCT GTAAGGAGTC CTTTGCAAAA 
TCCAAGAGTA CGTGGCTGTC ACCAGATGGA TTTGTATTTG CTGGATATAA GAGCAGCATT 
GAAAGCAACC TGCACCCTCG GATGCCTGAT GAGGCACGTG TGGAGGAGTT ACGAGAGAAA 
TGGCAGGAGA ATGCTCTGTT TGCTAACATG GCGCCAGTGC TGCCACGAGG CAGATGGAGC 
TGGGACAAAC GGCATGTTGA CTTCGAGCTC TACAAGAAGC CGCCTGAACT CCCCATGGCA 
ACAGCCACAC TGACAGATGC TACTCACTTT GTGCAAAATG TGTGTGATGA TACCAAGCTG 
AAGGTTCACT GGTGTTCTGC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293466.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148952.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X2, mRNA.

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CloneID	OGa41710
Clone ID Related Accession (Same CDS sequence)	XM_015293466.1 , XM_015293466.2
Accession Version	XM_015293466.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3600bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293466.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGATT TGAGGGTAAC 
GCCCAACTTC TGAAACAGAA AGCAGTTGCT GCTCCTTCAC ATAATGCAGC TTCAGAAGAA 
ACTGGGGAAA CAGCCAGCAA TGTGGGAATT CCGATGTGTC AAACAGACAA GACAGCTACC 
TTTAATGGTA GAAGAGATTC TGGTGCTAAA AAAGGCATGG ATACTACTAG GGCAAACATT 
GCATCACTGC AGTTGGATCT CATGCCTCTC CTCTCAGGAG AAAATTCAGT GATCAGTCGA 
CTAGAAGAAA ACAGTCCTAT TCTTTTAGAT GCTTATGTGT CTTTCACTGT AGAAACGCCT 
CTTCTGTCTG AAAGACAAAT ACGTGAATTG AATCCACTGG TTATAAAGAT CTGTTCAGCT 
ACCAGCCTCC CGAACACACC TATACCAATA GAAGTGCTAC AGAAATTGTG TGTTCCTACC 
TACTGCAAAT ACAAATTCCA CAATTTTCCA GCTCATCAGA CTCATGGACA AATCCACGGA 
ACTCATGTCT ACTTTAAGGA TGTTAATGTA TTTCTAACAG GAAGAATGAA ACCTGGGGAG 
TTACAGAGGT GTCTTAGAGG TCCCCCTTTG GAGATTGAAG TTCATGATCG AGATAGAAAG 
ATTGAAGACA ACAAAAAAGA GCCATGTCTA TTTGGGGAGG ATCACACTGA TGAAAATCTA 
GGAGAGTTGA GACCCCTCCC TTGCCAGTCT GCAATCTGTA ATTCTTTTGC AGAGGATGAA 
GTGTGGCATC CACATGGGGT AGCTAAAGTA AGTCTAAGTG ATCTATTGTT GGGTAAAAAG 
TACTTGACTG TTAGTGCACC CATTCGTAGC TGTTCAACTC CAAATACAGC TGCTTTCAAT 
AAAAAGAATA AAAGAAAGAA GATAGGGAAG AAGCATCCAC AGATCCTATT ACCCATGGGA 
CATTATCTAC AATCAGACTC GCTTTTGAAA TTAAGAGTGG AAATAGCAGT GCCATTGAGA 
ATGCATGCAG AAATTGATGA TGCTCAAGTG ACGGATTGCC CGTACAGTTG TATAGTCTAC 
ATTTTTGACT ACAGTAATTC ATTTTTATTA CATGATTTGG TGGAAGAGAT TACAGAAATC 
AATGCGAAAG CCCTTCAGCT GGACTGTTAT CCTCCACACT TGATCAGAAC ATCTCTTGCT 
ACATTAAAAC TAAAAAGCAT GCTTGAGAAG GTTTCTGAGT TAGATGTTGT TACTGGCTTT 
CACTTACTGG ATGGAGCTAC TCACCTCTTT GTCTTGGAGG GTTTAAAAGA CAAAGCCATC 
AAGAGACTCT GGGATAGGCA CTTTGAAAGA ACTTACAGAC ATGAAGATGG ACAACTGGAA 
ATACTATATA ATTCACAACT GTCTTTCAGT CAGCGCCTGT ATACCGACCT GGAAGCAATC 
TTTTATCGTT TCCACCTCTG TAAGCCCCTC TTCACTATAC TGAAACAGCC TCTGCTTTAT 
GTCAGGGGAG CAGTTCCTTG GGCGTGTTTC CAAGCCTTGT CCAGGCTCAG TTACCTCTGT 
CACAGTAAGA GGTTGAGGGA TGTCATTCAG GGGGATCTGC TGCCCTCAGC AGAAATGATC 
ACAGCGTTGA GTCGAGAGTA TGGAGTTCCA TTAGCTGATG AGGATCTCTT CACTCAGAAA 
CCTCCTCTAC TCTTATTTTC GTCTGATTAT ACACCACCAG GAAATGTAAG CAGAGAGAAA 
AAGGCACGAT ATTCTTCATT AGATCACTAC AACGAAAGAT ATGTGCTGTG GAAGAAAGAA 
ATAGCAGACA AAAAGTCTTT TGAGAGAAAC CATATTAGGG CTAACATTGA TGCTGTGCGC 
CAGCTTAAAG GAAAGATGAA AAGGCTGAAG TTTGAAACTT TCAGGATTTC TCCTGCCGAT 
GGCAAATCTG TTTATAACTA TAGCTCTCAG CGCCTGAATT CTGCAGAACT TGCAAAGAAG 
CATCTTCGCC AGGAAATGGC AAAGGATCCA AAAACAAGGT TCAGTTATTG TCACGAGTAT 
CTTTCAGCCA CGTTTGACCC AGTTGATGTG GTTGCTGCCT GTAAGGAGTC CTTTGCAAAA 
TCCAAGAGTA CGTGGCTGTC ACCAGATGGA TTTGTATTTG CTGGATATAA GAGCAGCATT 
GAAAGCAACC TGCACCCTCG GATGCCTGAT GAGGCACGTG TGGAGGAGTT ACGAGAGAAA 
TGGCAGGAGA ATGCTCTGTT TGCTAACATG GCGCCAGTGC TGCCACGAGG CAGATGGAGC 
TGGGACAAAC GGCATGTTGA CTTCGAGCTC TACAAGAAGC CGCCTGAACT CCCCATGGCA 
ACAGCCACAC TGACAGATGC TACTCACTTT GTGCAAAATG TGTGTGATGA TACCAAGCTG 
AAGGTTCACT GGTGTTCTGC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148952.1
CDS	1438..5037
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293466.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293466.2

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGATT TGAGGGTAAC 
GCCCAACTTC TGAAACAGAA AGCAGTTGCT GCTCCTTCAC ATAATGCAGC TTCAGAAGAA 
ACTGGGGAAA CAGCCAGCAA TGTGGGAATT CCGATGTGTC AAACAGACAA GACAGCTACC 
TTTAATGGTA GAAGAGATTC TGGTGCTAAA AAAGGCATGG ATACTACTAG GGCAAACATT 
GCATCACTGC AGTTGGATCT CATGCCTCTC CTCTCAGGAG AAAATTCAGT GATCAGTCGA 
CTAGAAGAAA ACAGTCCTAT TCTTTTAGAT GCTTATGTGT CTTTCACTGT AGAAACGCCT 
CTTCTGTCTG AAAGACAAAT ACGTGAATTG AATCCACTGG TTATAAAGAT CTGTTCAGCT 
ACCAGCCTCC CGAACACACC TATACCAATA GAAGTGCTAC AGAAATTGTG TGTTCCTACC 
TACTGCAAAT ACAAATTCCA CAATTTTCCA GCTCATCAGA CTCATGGACA AATCCACGGA 
ACTCATGTCT ACTTTAAGGA TGTTAATGTA TTTCTAACAG GAAGAATGAA ACCTGGGGAG 
TTACAGAGGT GTCTTAGAGG TCCCCCTTTG GAGATTGAAG TTCATGATCG AGATAGAAAG 
ATTGAAGACA ACAAAAAAGA GCCATGTCTA TTTGGGGAGG ATCACACTGA TGAAAATCTA 
GGAGAGTTGA GACCCCTCCC TTGCCAGTCT GCAATCTGTA ATTCTTTTGC AGAGGATGAA 
GTGTGGCATC CACATGGGGT AGCTAAAGTA AGTCTAAGTG ATCTATTGTT GGGTAAAAAG 
TACTTGACTG TTAGTGCACC CATTCGTAGC TGTTCAACTC CAAATACAGC TGCTTTCAAT 
AAAAAGAATA AAAGAAAGAA GATAGGGAAG AAGCATCCAC AGATCCTATT ACCCATGGGA 
CATTATCTAC AATCAGACTC GCTTTTGAAA TTAAGAGTGG AAATAGCAGT GCCATTGAGA 
ATGCATGCAG AAATTGATGA TGCTCAAGTG ACGGATTGCC CGTACAGTTG TATAGTCTAC 
ATTTTTGACT ACAGTAATTC ATTTTTATTA CATGATTTGG TGGAAGAGAT TACAGAAATC 
AATGCGAAAG CCCTTCAGCT GGACTGTTAT CCTCCACACT TGATCAGAAC ATCTCTTGCT 
ACATTAAAAC TAAAAAGCAT GCTTGAGAAG GTTTCTGAGT TAGATGTTGT TACTGGCTTT 
CACTTACTGG ATGGAGCTAC TCACCTCTTT GTCTTGGAGG GTTTAAAAGA CAAAGCCATC 
AAGAGACTCT GGGATAGGCA CTTTGAAAGA ACTTACAGAC ATGAAGATGG ACAACTGGAA 
ATACTATATA ATTCACAACT GTCTTTCAGT CAGCGCCTGT ATACCGACCT GGAAGCAATC 
TTTTATCGTT TCCACCTCTG TAAGCCCCTC TTCACTATAC TGAAACAGCC TCTGCTTTAT 
GTCAGGGGAG CAGTTCCTTG GGCGTGTTTC CAAGCCTTGT CCAGGCTCAG TTACCTCTGT 
CACAGTAAGA GGTTGAGGGA TGTCATTCAG GGGGATCTGC TGCCCTCAGC AGAAATGATC 
ACAGCGTTGA GTCGAGAGTA TGGAGTTCCA TTAGCTGATG AGGATCTCTT CACTCAGAAA 
CCTCCTCTAC TCTTATTTTC GTCTGATTAT ACACCACCAG GAAATGTAAG CAGAGAGAAA 
AAGGCACGAT ATTCTTCATT AGATCACTAC AACGAAAGAT ATGTGCTGTG GAAGAAAGAA 
ATAGCAGACA AAAAGTCTTT TGAGAGAAAC CATATTAGGG CTAACATTGA TGCTGTGCGC 
CAGCTTAAAG GAAAGATGAA AAGGCTGAAG TTTGAAACTT TCAGGATTTC TCCTGCCGAT 
GGCAAATCTG TTTATAACTA TAGCTCTCAG CGCCTGAATT CTGCAGAACT TGCAAAGAAG 
CATCTTCGCC AGGAAATGGC AAAGGATCCA AAAACAAGGT TCAGTTATTG TCACGAGTAT 
CTTTCAGCCA CGTTTGACCC AGTTGATGTG GTTGCTGCCT GTAAGGAGTC CTTTGCAAAA 
TCCAAGAGTA CGTGGCTGTC ACCAGATGGA TTTGTATTTG CTGGATATAA GAGCAGCATT 
GAAAGCAACC TGCACCCTCG GATGCCTGAT GAGGCACGTG TGGAGGAGTT ACGAGAGAAA 
TGGCAGGAGA ATGCTCTGTT TGCTAACATG GCGCCAGTGC TGCCACGAGG CAGATGGAGC 
TGGGACAAAC GGCATGTTGA CTTCGAGCTC TACAAGAAGC CGCCTGAACT CCCCATGGCA 
ACAGCCACAC TGACAGATGC TACTCACTTT GTGCAAAATG TGTGTGATGA TACCAAGCTG 
AAGGTTCACT GGTGTTCTGC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293467.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148953.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X3, mRNA.

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CloneID	OGa41711
Clone ID Related Accession (Same CDS sequence)	XM_015293467.2 , XM_015293467.1
Accession Version	XM_015293467.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293467.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148953.1
CDS	1438..4977
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293467.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293467.2

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293467.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148953.1 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X3, mRNA.

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CloneID	OGa41711
Clone ID Related Accession (Same CDS sequence)	XM_015293467.2 , XM_015293467.1
Accession Version	XM_015293467.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148953.1
CDS	58..3597
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293467.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293467.1

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGCAGCTGAG TTACGCGCAC GGGAGCCAGG AGCCACTTCT 
GAGCTACAGC ATCTCCAAGG ACTTCTGAAA GATGAGCCTC TGAAATTCCC ACTCAGGAAG 
CCAGGACTGA TTTTGAAGCC TAACCCAGCC CTCTCAGTGT TGGACTCTCA GAACAGCTCA 
GATGTCCTGC CAGGAAAGAA GAGCGTTTGC AGTGGCTTTG TGCCAGGCCT TCAAGACCAC 
CACAGCCGGA AGTGGAACAA GAACATTATC CCCTGGCACA ACAGAGAGCA TAAGAAGATT 
GAAAAACTTG AAGGCGCAGA TTTCAATTTA CTTGGCTCTA AACAATCAGT TTACAAGAGT 
GACCAAGCAG CAGGCAGACA CGCTACAACC TATGCACAAC AGGAAGACCC TCTCCAGAAT 
GATATTCTGG ATACAGCTAC TGCATCACAG CGTTGGGATG ACCCCCTCAG TGGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293468.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148954.1 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X4, mRNA.

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CloneID	OGa41712
Clone ID Related Accession (Same CDS sequence)	XM_015293468.1 , XM_015293468.2
Accession Version	XM_015293468.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGAGGA AACTGATCAG AGTGGACTGG GGACTTCCAG CTGTAATGTA 
CTACATCCTC CAAAGACGGA TGACAGTACA GTAAGAAACA GATTTGAGGG TAACGCCCAA 
CTTCTGAAAC AGAAAGCAGT TGCTGCTCCT TCACATAATG CAGCTTCAGA AGAAACTGGG 
GAAACAGCCA GCAATGTGGG AATTCCGATG TGTCAAACAG ACAAGACAGC TACCTTTAAT 
GGTAGAAGAG ATTCTGGTGC TAAAAAAGGC ATGGATACTA CTAGGGCAAA CATTGCATCA 
CTGCAGTTGG ATCTCATGCC TCTCCTCTCA GGAGAAAATT CAGTGATCAG TCGACTAGAA 
GAAAACAGTC CTATTCTTTT AGATGCTTAT GTGTCTTTCA CTGTAGAAAC GCCTCTTCTG 
TCTGAAAGAC AAATACGTGA ATTGAATCCA CTGGTTATAA AGATCTGTTC AGCTACCAGC 
CTCCCGAACA CACCTATACC AATAGAAGTG CTACAGAAAT TGTGTGTTCC TACCTACTGC 
AAATACAAAT TCCACAATTT TCCAGCTCAT CAGACTCATG GACAAATCCA CGGAACTCAT 
GTCTACTTTA AGGATGTTAA TGTATTTCTA ACAGGAAGAA TGAAACCTGG GGAGTTACAG 
AGGTGTCTTA GAGGTCCCCC TTTGGAGATT GAAGTTCATG ATCGAGATAG AAAGATTGAA 
GACAACAAAA AAGAGCCATG TCTATTTGGG GAGGATCACA CTGATGAAAA TCTAGGAGAG 
TTGAGACCCC TCCCTTGCCA GTCTGCAATC TGTAATTCTT TTGCAGAGGA TGAAGTGTGG 
CATCCACATG GGGTAGCTAA AGTAAGTCTA AGTGATCTAT TGTTGGGTAA AAAGTACTTG 
ACTGTTAGTG CACCCATTCG TAGCTGTTCA ACTCCAAATA CAGCTGCTTT CAATAAAAAG 
AATAAAAGAA AGAAGATAGG GAAGAAGCAT CCACAGATCC TATTACCCAT GGGACATTAT 
CTACAATCAG ACTCGCTTTT GAAATTAAGA GTGGAAATAG CAGTGCCATT GAGAATGCAT 
GCAGAAATTG ATGATGCTCA AGTGACGGAT TGCCCGTACA GTTGTATAGT CTACATTTTT 
GACTACAGTA ATTCATTTTT ATTACATGAT TTGGTGGAAG AGATTACAGA AATCAATGCG 
AAAGCCCTTC AGCTGGACTG TTATCCTCCA CACTTGATCA GAACATCTCT TGCTACATTA 
AAACTAAAAA GCATGCTTGA GAAGGTTTCT GAGTTAGATG TTGTTACTGG CTTTCACTTA 
CTGGATGGAG CTACTCACCT CTTTGTCTTG GAGGGTTTAA AAGACAAAGC CATCAAGAGA 
CTCTGGGATA GGCACTTTGA AAGAACTTAC AGACATGAAG ATGGACAACT GGAAATACTA 
TATAATTCAC AACTGTCTTT CAGTCAGCGC CTGTATACCG ACCTGGAAGC AATCTTTTAT 
CGTTTCCACC TCTGTAAGCC CCTCTTCACT ATACTGAAAC AGCCTCTGCT TTATGTCAGG 
GGAGCAGTTC CTTGGGCGTG TTTCCAAGCC TTGTCCAGGC TCAGTTACCT CTGTCACAGT 
AAGAGGTTGA GGGATGTCAT TCAGGGGGAT CTGCTGCCCT CAGCAGAAAT GATCACAGCG 
TTGAGTCGAG AGTATGGAGT TCCATTAGCT GATGAGGATC TCTTCACTCA GAAACCTCCT 
CTACTCTTAT TTTCGTCTGA TTATACACCA CCAGGAAATG TAAGCAGAGA GAAAAAGGCA 
CGATATTCTT CATTAGATCA CTACAACGAA AGATATGTGC TGTGGAAGAA AGAAATAGCA 
GACAAAAAGT CTTTTGAGAG AAACCATATT AGGGCTAACA TTGATGCTGT GCGCCAGCTT 
AAAGGAAAGA TGAAAAGGCT GAAGTTTGAA ACTTTCAGGA TTTCTCCTGC CGATGGCAAA 
TCTGTTTATA ACTATAGCTC TCAGCGCCTG AATTCTGCAG AACTTGCAAA GAAGCATCTT 
CGCCAGGAAA TGGCAAAGGA TCCAAAAACA AGGTTCAGTT ATTGTCACGA GTATCTTTCA 
GCCACGTTTG ACCCAGTTGA TGTGGTTGCT GCCTGTAAGG AGTCCTTTGC AAAATCCAAG 
AGTACGTGGC TGTCACCAGA TGGATTTGTA TTTGCTGGAT ATAAGAGCAG CATTGAAAGC 
AACCTGCACC CTCGGATGCC TGATGAGGCA CGTGTGGAGG AGTTACGAGA GAAATGGCAG 
GAGAATGCTC TGTTTGCTAA CATGGCGCCA GTGCTGCCAC GAGGCAGATG GAGCTGGGAC 
AAACGGCATG TTGACTTCGA GCTCTACAAG AAGCCGCCTG AACTCCCCAT GGCAACAGCC 
ACACTGACAG ATGCTACTCA CTTTGTGCAA AATGTGTGTG ATGATACCAA GCTGAAGGTT 
CACTGGTGTT CTGCAGCAGC TGAGTTACGC GCACGGGAGC CAGGAGCCAC TTCTGAGCTA 
CAGCATCTCC AAGGACTTCT GAAAGATGAG CCTCTGAAAT TCCCACTCAG GAAGCCAGGA 
CTGATTTTGA AGCCTAACCC AGCCCTCTCA GTGTTGGACT CTCAGAACAG CTCAGATGTC 
CTGCCAGGAA AGAAGAGCGT TTGCAGTGGC TTTGTGCCAG GCCTTCAAGA CCACCACAGC 
CGGAAGTGGA ACAAGAACAT TATCCCCTGG CACAACAGAG AGCATAAGAA GATTGAAAAA 
CTTGAAGGCG CAGATTTCAA TTTACTTGGC TCTAAACAAT CAGTTTACAA GAGTGACCAA 
GCAGCAGGCA GACACGCTAC AACCTATGCA CAACAGGAAG ACCCTCTCCA GAATGATATT 
CTGGATACAG CTACTGCATC ACAGCGTTGG GATGACCCCC TCAGTGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148954.1
CDS	58..3531
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293468.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293468.1

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGAGGA AACTGATCAG AGTGGACTGG GGACTTCCAG CTGTAATGTA 
CTACATCCTC CAAAGACGGA TGACAGTACA GTAAGAAACA GATTTGAGGG TAACGCCCAA 
CTTCTGAAAC AGAAAGCAGT TGCTGCTCCT TCACATAATG CAGCTTCAGA AGAAACTGGG 
GAAACAGCCA GCAATGTGGG AATTCCGATG TGTCAAACAG ACAAGACAGC TACCTTTAAT 
GGTAGAAGAG ATTCTGGTGC TAAAAAAGGC ATGGATACTA CTAGGGCAAA CATTGCATCA 
CTGCAGTTGG ATCTCATGCC TCTCCTCTCA GGAGAAAATT CAGTGATCAG TCGACTAGAA 
GAAAACAGTC CTATTCTTTT AGATGCTTAT GTGTCTTTCA CTGTAGAAAC GCCTCTTCTG 
TCTGAAAGAC AAATACGTGA ATTGAATCCA CTGGTTATAA AGATCTGTTC AGCTACCAGC 
CTCCCGAACA CACCTATACC AATAGAAGTG CTACAGAAAT TGTGTGTTCC TACCTACTGC 
AAATACAAAT TCCACAATTT TCCAGCTCAT CAGACTCATG GACAAATCCA CGGAACTCAT 
GTCTACTTTA AGGATGTTAA TGTATTTCTA ACAGGAAGAA TGAAACCTGG GGAGTTACAG 
AGGTGTCTTA GAGGTCCCCC TTTGGAGATT GAAGTTCATG ATCGAGATAG AAAGATTGAA 
GACAACAAAA AAGAGCCATG TCTATTTGGG GAGGATCACA CTGATGAAAA TCTAGGAGAG 
TTGAGACCCC TCCCTTGCCA GTCTGCAATC TGTAATTCTT TTGCAGAGGA TGAAGTGTGG 
CATCCACATG GGGTAGCTAA AGTAAGTCTA AGTGATCTAT TGTTGGGTAA AAAGTACTTG 
ACTGTTAGTG CACCCATTCG TAGCTGTTCA ACTCCAAATA CAGCTGCTTT CAATAAAAAG 
AATAAAAGAA AGAAGATAGG GAAGAAGCAT CCACAGATCC TATTACCCAT GGGACATTAT 
CTACAATCAG ACTCGCTTTT GAAATTAAGA GTGGAAATAG CAGTGCCATT GAGAATGCAT 
GCAGAAATTG ATGATGCTCA AGTGACGGAT TGCCCGTACA GTTGTATAGT CTACATTTTT 
GACTACAGTA ATTCATTTTT ATTACATGAT TTGGTGGAAG AGATTACAGA AATCAATGCG 
AAAGCCCTTC AGCTGGACTG TTATCCTCCA CACTTGATCA GAACATCTCT TGCTACATTA 
AAACTAAAAA GCATGCTTGA GAAGGTTTCT GAGTTAGATG TTGTTACTGG CTTTCACTTA 
CTGGATGGAG CTACTCACCT CTTTGTCTTG GAGGGTTTAA AAGACAAAGC CATCAAGAGA 
CTCTGGGATA GGCACTTTGA AAGAACTTAC AGACATGAAG ATGGACAACT GGAAATACTA 
TATAATTCAC AACTGTCTTT CAGTCAGCGC CTGTATACCG ACCTGGAAGC AATCTTTTAT 
CGTTTCCACC TCTGTAAGCC CCTCTTCACT ATACTGAAAC AGCCTCTGCT TTATGTCAGG 
GGAGCAGTTC CTTGGGCGTG TTTCCAAGCC TTGTCCAGGC TCAGTTACCT CTGTCACAGT 
AAGAGGTTGA GGGATGTCAT TCAGGGGGAT CTGCTGCCCT CAGCAGAAAT GATCACAGCG 
TTGAGTCGAG AGTATGGAGT TCCATTAGCT GATGAGGATC TCTTCACTCA GAAACCTCCT 
CTACTCTTAT TTTCGTCTGA TTATACACCA CCAGGAAATG TAAGCAGAGA GAAAAAGGCA 
CGATATTCTT CATTAGATCA CTACAACGAA AGATATGTGC TGTGGAAGAA AGAAATAGCA 
GACAAAAAGT CTTTTGAGAG AAACCATATT AGGGCTAACA TTGATGCTGT GCGCCAGCTT 
AAAGGAAAGA TGAAAAGGCT GAAGTTTGAA ACTTTCAGGA TTTCTCCTGC CGATGGCAAA 
TCTGTTTATA ACTATAGCTC TCAGCGCCTG AATTCTGCAG AACTTGCAAA GAAGCATCTT 
CGCCAGGAAA TGGCAAAGGA TCCAAAAACA AGGTTCAGTT ATTGTCACGA GTATCTTTCA 
GCCACGTTTG ACCCAGTTGA TGTGGTTGCT GCCTGTAAGG AGTCCTTTGC AAAATCCAAG 
AGTACGTGGC TGTCACCAGA TGGATTTGTA TTTGCTGGAT ATAAGAGCAG CATTGAAAGC 
AACCTGCACC CTCGGATGCC TGATGAGGCA CGTGTGGAGG AGTTACGAGA GAAATGGCAG 
GAGAATGCTC TGTTTGCTAA CATGGCGCCA GTGCTGCCAC GAGGCAGATG GAGCTGGGAC 
AAACGGCATG TTGACTTCGA GCTCTACAAG AAGCCGCCTG AACTCCCCAT GGCAACAGCC 
ACACTGACAG ATGCTACTCA CTTTGTGCAA AATGTGTGTG ATGATACCAA GCTGAAGGTT 
CACTGGTGTT CTGCAGCAGC TGAGTTACGC GCACGGGAGC CAGGAGCCAC TTCTGAGCTA 
CAGCATCTCC AAGGACTTCT GAAAGATGAG CCTCTGAAAT TCCCACTCAG GAAGCCAGGA 
CTGATTTTGA AGCCTAACCC AGCCCTCTCA GTGTTGGACT CTCAGAACAG CTCAGATGTC 
CTGCCAGGAA AGAAGAGCGT TTGCAGTGGC TTTGTGCCAG GCCTTCAAGA CCACCACAGC 
CGGAAGTGGA ACAAGAACAT TATCCCCTGG CACAACAGAG AGCATAAGAA GATTGAAAAA 
CTTGAAGGCG CAGATTTCAA TTTACTTGGC TCTAAACAAT CAGTTTACAA GAGTGACCAA 
GCAGCAGGCA GACACGCTAC AACCTATGCA CAACAGGAAG ACCCTCTCCA GAATGATATT 
CTGGATACAG CTACTGCATC ACAGCGTTGG GATGACCCCC TCAGTGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293468.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148954.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X4, mRNA.

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CloneID	OGa41712
Clone ID Related Accession (Same CDS sequence)	XM_015293468.1 , XM_015293468.2
Accession Version	XM_015293468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293468.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGAGGA AACTGATCAG AGTGGACTGG GGACTTCCAG CTGTAATGTA 
CTACATCCTC CAAAGACGGA TGACAGTACA GTAAGAAACA GATTTGAGGG TAACGCCCAA 
CTTCTGAAAC AGAAAGCAGT TGCTGCTCCT TCACATAATG CAGCTTCAGA AGAAACTGGG 
GAAACAGCCA GCAATGTGGG AATTCCGATG TGTCAAACAG ACAAGACAGC TACCTTTAAT 
GGTAGAAGAG ATTCTGGTGC TAAAAAAGGC ATGGATACTA CTAGGGCAAA CATTGCATCA 
CTGCAGTTGG ATCTCATGCC TCTCCTCTCA GGAGAAAATT CAGTGATCAG TCGACTAGAA 
GAAAACAGTC CTATTCTTTT AGATGCTTAT GTGTCTTTCA CTGTAGAAAC GCCTCTTCTG 
TCTGAAAGAC AAATACGTGA ATTGAATCCA CTGGTTATAA AGATCTGTTC AGCTACCAGC 
CTCCCGAACA CACCTATACC AATAGAAGTG CTACAGAAAT TGTGTGTTCC TACCTACTGC 
AAATACAAAT TCCACAATTT TCCAGCTCAT CAGACTCATG GACAAATCCA CGGAACTCAT 
GTCTACTTTA AGGATGTTAA TGTATTTCTA ACAGGAAGAA TGAAACCTGG GGAGTTACAG 
AGGTGTCTTA GAGGTCCCCC TTTGGAGATT GAAGTTCATG ATCGAGATAG AAAGATTGAA 
GACAACAAAA AAGAGCCATG TCTATTTGGG GAGGATCACA CTGATGAAAA TCTAGGAGAG 
TTGAGACCCC TCCCTTGCCA GTCTGCAATC TGTAATTCTT TTGCAGAGGA TGAAGTGTGG 
CATCCACATG GGGTAGCTAA AGTAAGTCTA AGTGATCTAT TGTTGGGTAA AAAGTACTTG 
ACTGTTAGTG CACCCATTCG TAGCTGTTCA ACTCCAAATA CAGCTGCTTT CAATAAAAAG 
AATAAAAGAA AGAAGATAGG GAAGAAGCAT CCACAGATCC TATTACCCAT GGGACATTAT 
CTACAATCAG ACTCGCTTTT GAAATTAAGA GTGGAAATAG CAGTGCCATT GAGAATGCAT 
GCAGAAATTG ATGATGCTCA AGTGACGGAT TGCCCGTACA GTTGTATAGT CTACATTTTT 
GACTACAGTA ATTCATTTTT ATTACATGAT TTGGTGGAAG AGATTACAGA AATCAATGCG 
AAAGCCCTTC AGCTGGACTG TTATCCTCCA CACTTGATCA GAACATCTCT TGCTACATTA 
AAACTAAAAA GCATGCTTGA GAAGGTTTCT GAGTTAGATG TTGTTACTGG CTTTCACTTA 
CTGGATGGAG CTACTCACCT CTTTGTCTTG GAGGGTTTAA AAGACAAAGC CATCAAGAGA 
CTCTGGGATA GGCACTTTGA AAGAACTTAC AGACATGAAG ATGGACAACT GGAAATACTA 
TATAATTCAC AACTGTCTTT CAGTCAGCGC CTGTATACCG ACCTGGAAGC AATCTTTTAT 
CGTTTCCACC TCTGTAAGCC CCTCTTCACT ATACTGAAAC AGCCTCTGCT TTATGTCAGG 
GGAGCAGTTC CTTGGGCGTG TTTCCAAGCC TTGTCCAGGC TCAGTTACCT CTGTCACAGT 
AAGAGGTTGA GGGATGTCAT TCAGGGGGAT CTGCTGCCCT CAGCAGAAAT GATCACAGCG 
TTGAGTCGAG AGTATGGAGT TCCATTAGCT GATGAGGATC TCTTCACTCA GAAACCTCCT 
CTACTCTTAT TTTCGTCTGA TTATACACCA CCAGGAAATG TAAGCAGAGA GAAAAAGGCA 
CGATATTCTT CATTAGATCA CTACAACGAA AGATATGTGC TGTGGAAGAA AGAAATAGCA 
GACAAAAAGT CTTTTGAGAG AAACCATATT AGGGCTAACA TTGATGCTGT GCGCCAGCTT 
AAAGGAAAGA TGAAAAGGCT GAAGTTTGAA ACTTTCAGGA TTTCTCCTGC CGATGGCAAA 
TCTGTTTATA ACTATAGCTC TCAGCGCCTG AATTCTGCAG AACTTGCAAA GAAGCATCTT 
CGCCAGGAAA TGGCAAAGGA TCCAAAAACA AGGTTCAGTT ATTGTCACGA GTATCTTTCA 
GCCACGTTTG ACCCAGTTGA TGTGGTTGCT GCCTGTAAGG AGTCCTTTGC AAAATCCAAG 
AGTACGTGGC TGTCACCAGA TGGATTTGTA TTTGCTGGAT ATAAGAGCAG CATTGAAAGC 
AACCTGCACC CTCGGATGCC TGATGAGGCA CGTGTGGAGG AGTTACGAGA GAAATGGCAG 
GAGAATGCTC TGTTTGCTAA CATGGCGCCA GTGCTGCCAC GAGGCAGATG GAGCTGGGAC 
AAACGGCATG TTGACTTCGA GCTCTACAAG AAGCCGCCTG AACTCCCCAT GGCAACAGCC 
ACACTGACAG ATGCTACTCA CTTTGTGCAA AATGTGTGTG ATGATACCAA GCTGAAGGTT 
CACTGGTGTT CTGCAGCAGC TGAGTTACGC GCACGGGAGC CAGGAGCCAC TTCTGAGCTA 
CAGCATCTCC AAGGACTTCT GAAAGATGAG CCTCTGAAAT TCCCACTCAG GAAGCCAGGA 
CTGATTTTGA AGCCTAACCC AGCCCTCTCA GTGTTGGACT CTCAGAACAG CTCAGATGTC 
CTGCCAGGAA AGAAGAGCGT TTGCAGTGGC TTTGTGCCAG GCCTTCAAGA CCACCACAGC 
CGGAAGTGGA ACAAGAACAT TATCCCCTGG CACAACAGAG AGCATAAGAA GATTGAAAAA 
CTTGAAGGCG CAGATTTCAA TTTACTTGGC TCTAAACAAT CAGTTTACAA GAGTGACCAA 
GCAGCAGGCA GACACGCTAC AACCTATGCA CAACAGGAAG ACCCTCTCCA GAATGATATT 
CTGGATACAG CTACTGCATC ACAGCGTTGG GATGACCCCC TCAGTGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148954.1
CDS	1438..4911
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_015293468.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293468.2

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGAGGA AACTGATCAG AGTGGACTGG GGACTTCCAG CTGTAATGTA 
CTACATCCTC CAAAGACGGA TGACAGTACA GTAAGAAACA GATTTGAGGG TAACGCCCAA 
CTTCTGAAAC AGAAAGCAGT TGCTGCTCCT TCACATAATG CAGCTTCAGA AGAAACTGGG 
GAAACAGCCA GCAATGTGGG AATTCCGATG TGTCAAACAG ACAAGACAGC TACCTTTAAT 
GGTAGAAGAG ATTCTGGTGC TAAAAAAGGC ATGGATACTA CTAGGGCAAA CATTGCATCA 
CTGCAGTTGG ATCTCATGCC TCTCCTCTCA GGAGAAAATT CAGTGATCAG TCGACTAGAA 
GAAAACAGTC CTATTCTTTT AGATGCTTAT GTGTCTTTCA CTGTAGAAAC GCCTCTTCTG 
TCTGAAAGAC AAATACGTGA ATTGAATCCA CTGGTTATAA AGATCTGTTC AGCTACCAGC 
CTCCCGAACA CACCTATACC AATAGAAGTG CTACAGAAAT TGTGTGTTCC TACCTACTGC 
AAATACAAAT TCCACAATTT TCCAGCTCAT CAGACTCATG GACAAATCCA CGGAACTCAT 
GTCTACTTTA AGGATGTTAA TGTATTTCTA ACAGGAAGAA TGAAACCTGG GGAGTTACAG 
AGGTGTCTTA GAGGTCCCCC TTTGGAGATT GAAGTTCATG ATCGAGATAG AAAGATTGAA 
GACAACAAAA AAGAGCCATG TCTATTTGGG GAGGATCACA CTGATGAAAA TCTAGGAGAG 
TTGAGACCCC TCCCTTGCCA GTCTGCAATC TGTAATTCTT TTGCAGAGGA TGAAGTGTGG 
CATCCACATG GGGTAGCTAA AGTAAGTCTA AGTGATCTAT TGTTGGGTAA AAAGTACTTG 
ACTGTTAGTG CACCCATTCG TAGCTGTTCA ACTCCAAATA CAGCTGCTTT CAATAAAAAG 
AATAAAAGAA AGAAGATAGG GAAGAAGCAT CCACAGATCC TATTACCCAT GGGACATTAT 
CTACAATCAG ACTCGCTTTT GAAATTAAGA GTGGAAATAG CAGTGCCATT GAGAATGCAT 
GCAGAAATTG ATGATGCTCA AGTGACGGAT TGCCCGTACA GTTGTATAGT CTACATTTTT 
GACTACAGTA ATTCATTTTT ATTACATGAT TTGGTGGAAG AGATTACAGA AATCAATGCG 
AAAGCCCTTC AGCTGGACTG TTATCCTCCA CACTTGATCA GAACATCTCT TGCTACATTA 
AAACTAAAAA GCATGCTTGA GAAGGTTTCT GAGTTAGATG TTGTTACTGG CTTTCACTTA 
CTGGATGGAG CTACTCACCT CTTTGTCTTG GAGGGTTTAA AAGACAAAGC CATCAAGAGA 
CTCTGGGATA GGCACTTTGA AAGAACTTAC AGACATGAAG ATGGACAACT GGAAATACTA 
TATAATTCAC AACTGTCTTT CAGTCAGCGC CTGTATACCG ACCTGGAAGC AATCTTTTAT 
CGTTTCCACC TCTGTAAGCC CCTCTTCACT ATACTGAAAC AGCCTCTGCT TTATGTCAGG 
GGAGCAGTTC CTTGGGCGTG TTTCCAAGCC TTGTCCAGGC TCAGTTACCT CTGTCACAGT 
AAGAGGTTGA GGGATGTCAT TCAGGGGGAT CTGCTGCCCT CAGCAGAAAT GATCACAGCG 
TTGAGTCGAG AGTATGGAGT TCCATTAGCT GATGAGGATC TCTTCACTCA GAAACCTCCT 
CTACTCTTAT TTTCGTCTGA TTATACACCA CCAGGAAATG TAAGCAGAGA GAAAAAGGCA 
CGATATTCTT CATTAGATCA CTACAACGAA AGATATGTGC TGTGGAAGAA AGAAATAGCA 
GACAAAAAGT CTTTTGAGAG AAACCATATT AGGGCTAACA TTGATGCTGT GCGCCAGCTT 
AAAGGAAAGA TGAAAAGGCT GAAGTTTGAA ACTTTCAGGA TTTCTCCTGC CGATGGCAAA 
TCTGTTTATA ACTATAGCTC TCAGCGCCTG AATTCTGCAG AACTTGCAAA GAAGCATCTT 
CGCCAGGAAA TGGCAAAGGA TCCAAAAACA AGGTTCAGTT ATTGTCACGA GTATCTTTCA 
GCCACGTTTG ACCCAGTTGA TGTGGTTGCT GCCTGTAAGG AGTCCTTTGC AAAATCCAAG 
AGTACGTGGC TGTCACCAGA TGGATTTGTA TTTGCTGGAT ATAAGAGCAG CATTGAAAGC 
AACCTGCACC CTCGGATGCC TGATGAGGCA CGTGTGGAGG AGTTACGAGA GAAATGGCAG 
GAGAATGCTC TGTTTGCTAA CATGGCGCCA GTGCTGCCAC GAGGCAGATG GAGCTGGGAC 
AAACGGCATG TTGACTTCGA GCTCTACAAG AAGCCGCCTG AACTCCCCAT GGCAACAGCC 
ACACTGACAG ATGCTACTCA CTTTGTGCAA AATGTGTGTG ATGATACCAA GCTGAAGGTT 
CACTGGTGTT CTGCAGCAGC TGAGTTACGC GCACGGGAGC CAGGAGCCAC TTCTGAGCTA 
CAGCATCTCC AAGGACTTCT GAAAGATGAG CCTCTGAAAT TCCCACTCAG GAAGCCAGGA 
CTGATTTTGA AGCCTAACCC AGCCCTCTCA GTGTTGGACT CTCAGAACAG CTCAGATGTC 
CTGCCAGGAA AGAAGAGCGT TTGCAGTGGC TTTGTGCCAG GCCTTCAAGA CCACCACAGC 
CGGAAGTGGA ACAAGAACAT TATCCCCTGG CACAACAGAG AGCATAAGAA GATTGAAAAA 
CTTGAAGGCG CAGATTTCAA TTTACTTGGC TCTAAACAAT CAGTTTACAA GAGTGACCAA 
GCAGCAGGCA GACACGCTAC AACCTATGCA CAACAGGAAG ACCCTCTCCA GAATGATATT 
CTGGATACAG CTACTGCATC ACAGCGTTGG GATGACCCCC TCAGTGGACA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293469.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148955.1 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X5, mRNA.

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CloneID	OGa41713
Clone ID Related Accession (Same CDS sequence)	XM_015293469.1 , XM_015293469.2
Accession Version	XM_015293469.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148955.1
CDS	58..3513
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFK

Target ORF information:

RefSeq Version	XM_015293469.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293469.1

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_015293469.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015148955.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X6, mRNA.

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CloneID	OGa41713
Clone ID Related Accession (Same CDS sequence)	XM_015293469.1 , XM_015293469.2
Accession Version	XM_015293469.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293469.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015148955.1
CDS	1438..4893
Translation	MEEGGGEELGTARAESGCGGSERGSSPAEGARQLSSPRSVPCVLTVSLSIPALATKQKQMTTNPPDRRGKGAPADELKTKRCYCIECFLLPDDLVPRKMDLVVVGMVAKLFAESDSKTIMPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFK

Target ORF information:

RefSeq Version	XM_015293469.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293469.2

ORF Insert Sequence:

ATGGAGGAGG GCGGTGGAGA GGAGCTGGGC ACGGCTCGTG CTGAGAGCGG CTGTGGCGGC 
TCGGAGCGGG GCAGCAGCCC TGCGGAAGGC GCGCGGCAGC TGAGCAGCCC CCGCTCCGTG 
CCCTGCGTGC TCACCGTGTC GCTCTCTATC CCTGCCTTGG CCACGAAGCA AAAGCAGATG 
ACAACCAATC CTCCTGATAG GCGAGGAAAG GGAGCTCCAG CTGATGAACT AAAGACAAAG 
CGTTGCTATT GCATTGAGTG CTTCCTTTTA CCAGATGATC TCGTGCCTAG AAAAATGGAT 
TTGGTGGTGG TTGGGATGGT TGCAAAGCTA TTTGCAGAGT CTGATTCCAA GACTATTATG 
CCATGGCTTG AAAATGACCA GATGTGGATC TCGTGGAGCC ATAATACAAA TGTCAATGTC 
ACTAATGAAT TTCTAGTAAA ATTAAGAGAT CATAAAATAA CCTTAAAACT TTGGGACACC 
AAGGACCAAG TTTTCTCAGA AGCAAGGCTT ACTAAACCAG ACATGTCCTC AATAGAAGGC 
GATGCGGAGG CTGTTGATGG AGTAAAACAC ACAGTGCTGC AGCAAAGGAG ATTGTTTGAA 
GGGAGTCAGC CAGCACCCAG CTGTACAAAA ATTAGAGAAG CAAAGGAATC CAAGGCACAG 
AAAGAGTCTT CAGCCCTTCC AATCAAAGAG GAAACTGATC AGAGTGGACT GGGGACTTCC 
AGCTGTAATG TACTACATCC TCCAAAGACG GATGACAGTA CAGTAAGAAA CAGATTTGAG 
GGTAACGCCC AACTTCTGAA ACAGAAAGCA GTTGCTGCTC CTTCACATAA TGCAGCTTCA 
GAAGAAACTG GGGAAACAGC CAGCAATGTG GGAATTCCGA TGTGTCAAAC AGACAAGACA 
GCTACCTTTA ATGGTAGAAG AGATTCTGGT GCTAAAAAAG GCATGGATAC TACTAGGGCA 
AACATTGCAT CACTGCAGTT GGATCTCATG CCTCTCCTCT CAGGAGAAAA TTCAGTGATC 
AGTCGACTAG AAGAAAACAG TCCTATTCTT TTAGATGCTT ATGTGTCTTT CACTGTAGAA 
ACGCCTCTTC TGTCTGAAAG ACAAATACGT GAATTGAATC CACTGGTTAT AAAGATCTGT 
TCAGCTACCA GCCTCCCGAA CACACCTATA CCAATAGAAG TGCTACAGAA ATTGTGTGTT 
CCTACCTACT GCAAATACAA ATTCCACAAT TTTCCAGCTC ATCAGACTCA TGGACAAATC 
CACGGAACTC ATGTCTACTT TAAGGATGTT AATGTATTTC TAACAGGAAG AATGAAACCT 
GGGGAGTTAC AGAGGTGTCT TAGAGGTCCC CCTTTGGAGA TTGAAGTTCA TGATCGAGAT 
AGAAAGATTG AAGACAACAA AAAAGAGCCA TGTCTATTTG GGGAGGATCA CACTGATGAA 
AATCTAGGAG AGTTGAGACC CCTCCCTTGC CAGTCTGCAA TCTGTAATTC TTTTGCAGAG 
GATGAAGTGT GGCATCCACA TGGGGTAGCT AAAGTAAGTC TAAGTGATCT ATTGTTGGGT 
AAAAAGTACT TGACTGTTAG TGCACCCATT CGTAGCTGTT CAACTCCAAA TACAGCTGCT 
TTCAATAAAA AGAATAAAAG AAAGAAGATA GGGAAGAAGC ATCCACAGAT CCTATTACCC 
ATGGGACATT ATCTACAATC AGACTCGCTT TTGAAATTAA GAGTGGAAAT AGCAGTGCCA 
TTGAGAATGC ATGCAGAAAT TGATGATGCT CAAGTGACGG ATTGCCCGTA CAGTTGTATA 
GTCTACATTT TTGACTACAG TAATTCATTT TTATTACATG ATTTGGTGGA AGAGATTACA 
GAAATCAATG CGAAAGCCCT TCAGCTGGAC TGTTATCCTC CACACTTGAT CAGAACATCT 
CTTGCTACAT TAAAACTAAA AAGCATGCTT GAGAAGGTTT CTGAGTTAGA TGTTGTTACT 
GGCTTTCACT TACTGGATGG AGCTACTCAC CTCTTTGTCT TGGAGGGTTT AAAAGACAAA 
GCCATCAAGA GACTCTGGGA TAGGCACTTT GAAAGAACTT ACAGACATGA AGATGGACAA 
CTGGAAATAC TATATAATTC ACAACTGTCT TTCAGTCAGC GCCTGTATAC CGACCTGGAA 
GCAATCTTTT ATCGTTTCCA CCTCTGTAAG CCCCTCTTCA CTATACTGAA ACAGCCTCTG 
CTTTATGTCA GGGGAGCAGT TCCTTGGGCG TGTTTCCAAG CCTTGTCCAG GCTCAGTTAC 
CTCTGTCACA GTAAGAGGTT GAGGGATGTC ATTCAGGGGG ATCTGCTGCC CTCAGCAGAA 
ATGATCACAG CGTTGAGTCG AGAGTATGGA GTTCCATTAG CTGATGAGGA TCTCTTCACT 
CAGAAACCTC CTCTACTCTT ATTTTCGTCT GATTATACAC CACCAGGAAA TGTAAGCAGA 
GAGAAAAAGG CACGATATTC TTCATTAGAT CACTACAACG AAAGATATGT GCTGTGGAAG 
AAAGAAATAG CAGACAAAAA GTCTTTTGAG AGAAACCATA TTAGGGCTAA CATTGATGCT 
GTGCGCCAGC TTAAAGGAAA GATGAAAAGG CTGAAGTTTG AAACTTTCAG GATTTCTCCT 
GCCGATGGCA AATCTGTTTA TAACTATAGC TCTCAGCGCC TGAATTCTGC AGAACTTGCA 
AAGAAGCATC TTCGCCAGGA AATGGCAAAG GATCCAAAAA CAAGGTTCAG TTATTGTCAC 
GAGTATCTTT CAGCCACGTT TGACCCAGTT GATGTGGTTG CTGCCTGTAA GGAGTCCTTT 
GCAAAATCCA AGAGTACGTG GCTGTCACCA GATGGATTTG TATTTGCTGG ATATAAGAGC 
AGCATTGAAA GCAACCTGCA CCCTCGGATG CCTGATGAGG CACGTGTGGA GGAGTTACGA 
GAGAAATGGC AGGAGAATGC TCTGTTTGCT AACATGGCGC CAGTGCTGCC ACGAGGCAGA 
TGGAGCTGGG ACAAACGGCA TGTTGACTTC GAGCTCTACA AGAAGCCGCC TGAACTCCCC 
ATGGCAACAG CCACACTGAC AGATGCTACT CACTTTGTGC AAAATGTGTG TGATGATACC 
AAGCTGAAGG TTCACTGGTG TTCTGCAGCA GCTGAGTTAC GCGCACGGGA GCCAGGAGCC 
ACTTCTGAGC TACAGCATCT CCAAGGACTT CTGAAAGATG AGCCTCTGAA ATTCCCACTC 
AGGAAGCCAG GACTGATTTT GAAGCCTAAC CCAGCCCTCT CAGTGTTGGA CTCTCAGAAC 
AGCTCAGATG TCCTGCCAGG AAAGAAGAGC GTTTGCAGTG GCTTTGTGCC AGGCCTTCAA 
GACCACCACA GCCGGAAGTG GAACAAGAAC ATTATCCCCT GGCACAACAG AGAGCATAAG 
AAGATTGAAA AACTTGAAGG CGCAGATTTC AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_025154698.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010466.1 Gallus gallus KIAA1257 (KIAA1257), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OGa41714
Clone ID Related Accession (Same CDS sequence)	XM_025154698.1 , XM_004944522.2
Accession Version	XM_025154698.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006099.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG AAACAGATTT 
GAGGGTAACG CCCAACTTCT GAAACAGAAA GCAGTTGCTG CTCCTTCACA TAATGCAGCT 
TCAGAAGAAA CTGGGGAAAC AGCCAGCAAT GTGGGAATTC CGATGTGTCA AACAGACAAG 
ACAGCTACCT TTAATGGTAG AAGAGATTCT GGTGCTAAAA AAGGCATGGA TACTACTAGG 
GCAAACATTG CATCACTGCA GTTGGATCTC ATGCCTCTCC TCTCAGGAGA AAATTCAGTG 
ATCAGTCGAC TAGAAGAAAA CAGTCCTATT CTTTTAGATG CTTATGTGTC TTTCACTGTA 
GAAACGCCTC TTCTGTCTGA AAGACAAATA CGTGAATTGA ATCCACTGGT TATAAAGATC 
TGTTCAGCTA CCAGCCTCCC GAACACACCT ATACCAATAG AAGTGCTACA GAAATTGTGT 
GTTCCTACCT ACTGCAAATA CAAATTCCAC AATTTTCCAG CTCATCAGAC TCATGGACAA 
ATCCACGGAA CTCATGTCTA CTTTAAGGAT GTTAATGTAT TTCTAACAGG AAGAATGAAA 
CCTGGGGAGT TACAGAGGTG TCTTAGAGGT CCCCCTTTGG AGATTGAAGT TCATGATCGA 
GATAGAAAGA TTGAAGACAA CAAAAAAGAG CCATGTCTAT TTGGGGAGGA TCACACTGAT 
GAAAATCTAG GAGAGTTGAG ACCCCTCCCT TGCCAGTCTG CAATCTGTAA TTCTTTTGCA 
GAGGATGAAG TGTGGCATCC ACATGGGGTA GCTAAAGTAA GTCTAAGTGA TCTATTGTTG 
GGTAAAAAGT ACTTGACTGT TAGTGCACCC ATTCGTAGCT GTTCAACTCC AAATACAGCT 
GCTTTCAATA AAAAGAATAA AAGAAAGAAG ATAGGGAAGA AGCATCCACA GATCCTATTA 
CCCATGGGAC ATTATCTACA ATCAGACTCG CTTTTGAAAT TAAGAGTGGA AATAGCAGTG 
CCATTGAGAA TGCATGCAGA AATTGATGAT GCTCAAGTGA CGGATTGCCC GTACAGTTGT 
ATAGTCTACA TTTTTGACTA CAGTAATTCA TTTTTATTAC ATGATTTGGT GGAAGAGATT 
ACAGAAATCA ATGCGAAAGC CCTTCAGCTG GACTGTTATC CTCCACACTT GATCAGAACA 
TCTCTTGCTA CATTAAAACT AAAAAGCATG CTTGAGAAGG TTTCTGAGTT AGATGTTGTT 
ACTGGCTTTC ACTTACTGGA TGGAGCTACT CACCTCTTTG TCTTGGAGGG TTTAAAAGAC 
AAAGCCATCA AGAGACTCTG GGATAGGCAC TTTGAAAGAA CTTACAGACA TGAAGATGGA 
CAACTGGAAA TACTATATAA TTCACAACTG TCTTTCAGTC AGCGCCTGTA TACCGACCTG 
GAAGCAATCT TTTATCGTTT CCACCTCTGT AAGCCCCTCT TCACTATACT GAAACAGCCT 
CTGCTTTATG TCAGGGGAGC AGTTCCTTGG GCGTGTTTCC AAGCCTTGTC CAGGCTCAGT 
TACCTCTGTC ACAGTAAGAG GTTGAGGGAT GTCATTCAGG GGGATCTGCT GCCCTCAGCA 
GAAATGATCA CAGCGTTGAG TCGAGAGTAT GGAGTTCCAT TAGCTGATGA GGATCTCTTC 
ACTCAGAAAC CTCCTCTACT CTTATTTTCG TCTGATTATA CACCACCAGG AAATGTAAGC 
AGAGAGAAAA AGGCACGATA TTCTTCATTA GATCACTACA ACGAAAGATA TGTGCTGTGG 
AAGAAAGAAA TAGCAGACAA AAAGTCTTTT GAGAGAAACC ATATTAGGGC TAACATTGAT 
GCTGTGCGCC AGCTTAAAGG AAAGATGAAA AGGCTGAAGT TTGAAACTTT CAGGATTTCT 
CCTGCCGATG GCAAATCTGT TTATAACTAT AGCTCTCAGC GCCTGAATTC TGCAGAACTT 
GCAAAGAAGC ATCTTCGCCA GGAAATGGCA AAGGATCCAA AAACAAGGTT CAGTTATTGT 
CACGAGTATC TTTCAGCCAC GTTTGACCCA GTTGATGTGG TTGCTGCCTG TAAGGAGTCC 
TTTGCAAAAT CCAAGAGTAC GTGGCTGTCA CCAGATGGAT TTGTATTTGC TGGATATAAG 
AGCAGCATTG AAAGCAACCT GCACCCTCGG ATGCCTGATG AGGCACGTGT GGAGGAGTTA 
CGAGAGAAAT GGCAGGAGAA TGCTCTGTTT GCTAACATGG CGCCAGTGCT GCCACGAGGC 
AGATGGAGCT GGGACAAACG GCATGTTGAC TTCGAGCTCT ACAAGAAGCC GCCTGAACTC 
CCCATGGCAA CAGCCACACT GACAGATGCT ACTCACTTTG TGCAAAATGT GTGTGATGAT 
ACCAAGCTGA AGGTTCACTG GTGTTCTGCA GCAGCTGAGT TACGCGCACG GGAGCCAGGA 
GCCACTTCTG AGCTACAGCA TCTCCAAGGA CTTCTGAAAG ATGAGCCTCT GAAATTCCCA 
CTCAGGAAGC CAGGACTGAT TTTGAAGCCT AACCCAGCCC TCTCAGTGTT GGACTCTCAG 
AACAGCTCAG ATGTCCTGCC AGGAAAGAAG AGCGTTTGCA GTGGCTTTGT GCCAGGCCTT 
CAAGACCACC ACAGCCGGAA GTGGAACAAG AACATTATCC CCTGGCACAA CAGAGAGCAT 
AAGAAGATTG AAAAACTTGA AGGCGCAGAT TTCAATTTAC TTGGCTCTAA ACAATCAGTT 
TACAAGAGTG ACCAAGCAGC AGGCAGACAC GCTACAACCT ATGCACAACA GGAAGACCCT 
CTCCAGAATG ATATTCTGGA TACAGCTACT GCATCACAGC GTTGGGATGA CCCCCTCAGT 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010466.1
CDS	134..3382
Translation	MPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_025154698.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus KIAA1257 (KIAA1257), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154698.1

ORF Insert Sequence:

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG AAACAGATTT 
GAGGGTAACG CCCAACTTCT GAAACAGAAA GCAGTTGCTG CTCCTTCACA TAATGCAGCT 
TCAGAAGAAA CTGGGGAAAC AGCCAGCAAT GTGGGAATTC CGATGTGTCA AACAGACAAG 
ACAGCTACCT TTAATGGTAG AAGAGATTCT GGTGCTAAAA AAGGCATGGA TACTACTAGG 
GCAAACATTG CATCACTGCA GTTGGATCTC ATGCCTCTCC TCTCAGGAGA AAATTCAGTG 
ATCAGTCGAC TAGAAGAAAA CAGTCCTATT CTTTTAGATG CTTATGTGTC TTTCACTGTA 
GAAACGCCTC TTCTGTCTGA AAGACAAATA CGTGAATTGA ATCCACTGGT TATAAAGATC 
TGTTCAGCTA CCAGCCTCCC GAACACACCT ATACCAATAG AAGTGCTACA GAAATTGTGT 
GTTCCTACCT ACTGCAAATA CAAATTCCAC AATTTTCCAG CTCATCAGAC TCATGGACAA 
ATCCACGGAA CTCATGTCTA CTTTAAGGAT GTTAATGTAT TTCTAACAGG AAGAATGAAA 
CCTGGGGAGT TACAGAGGTG TCTTAGAGGT CCCCCTTTGG AGATTGAAGT TCATGATCGA 
GATAGAAAGA TTGAAGACAA CAAAAAAGAG CCATGTCTAT TTGGGGAGGA TCACACTGAT 
GAAAATCTAG GAGAGTTGAG ACCCCTCCCT TGCCAGTCTG CAATCTGTAA TTCTTTTGCA 
GAGGATGAAG TGTGGCATCC ACATGGGGTA GCTAAAGTAA GTCTAAGTGA TCTATTGTTG 
GGTAAAAAGT ACTTGACTGT TAGTGCACCC ATTCGTAGCT GTTCAACTCC AAATACAGCT 
GCTTTCAATA AAAAGAATAA AAGAAAGAAG ATAGGGAAGA AGCATCCACA GATCCTATTA 
CCCATGGGAC ATTATCTACA ATCAGACTCG CTTTTGAAAT TAAGAGTGGA AATAGCAGTG 
CCATTGAGAA TGCATGCAGA AATTGATGAT GCTCAAGTGA CGGATTGCCC GTACAGTTGT 
ATAGTCTACA TTTTTGACTA CAGTAATTCA TTTTTATTAC ATGATTTGGT GGAAGAGATT 
ACAGAAATCA ATGCGAAAGC CCTTCAGCTG GACTGTTATC CTCCACACTT GATCAGAACA 
TCTCTTGCTA CATTAAAACT AAAAAGCATG CTTGAGAAGG TTTCTGAGTT AGATGTTGTT 
ACTGGCTTTC ACTTACTGGA TGGAGCTACT CACCTCTTTG TCTTGGAGGG TTTAAAAGAC 
AAAGCCATCA AGAGACTCTG GGATAGGCAC TTTGAAAGAA CTTACAGACA TGAAGATGGA 
CAACTGGAAA TACTATATAA TTCACAACTG TCTTTCAGTC AGCGCCTGTA TACCGACCTG 
GAAGCAATCT TTTATCGTTT CCACCTCTGT AAGCCCCTCT TCACTATACT GAAACAGCCT 
CTGCTTTATG TCAGGGGAGC AGTTCCTTGG GCGTGTTTCC AAGCCTTGTC CAGGCTCAGT 
TACCTCTGTC ACAGTAAGAG GTTGAGGGAT GTCATTCAGG GGGATCTGCT GCCCTCAGCA 
GAAATGATCA CAGCGTTGAG TCGAGAGTAT GGAGTTCCAT TAGCTGATGA GGATCTCTTC 
ACTCAGAAAC CTCCTCTACT CTTATTTTCG TCTGATTATA CACCACCAGG AAATGTAAGC 
AGAGAGAAAA AGGCACGATA TTCTTCATTA GATCACTACA ACGAAAGATA TGTGCTGTGG 
AAGAAAGAAA TAGCAGACAA AAAGTCTTTT GAGAGAAACC ATATTAGGGC TAACATTGAT 
GCTGTGCGCC AGCTTAAAGG AAAGATGAAA AGGCTGAAGT TTGAAACTTT CAGGATTTCT 
CCTGCCGATG GCAAATCTGT TTATAACTAT AGCTCTCAGC GCCTGAATTC TGCAGAACTT 
GCAAAGAAGC ATCTTCGCCA GGAAATGGCA AAGGATCCAA AAACAAGGTT CAGTTATTGT 
CACGAGTATC TTTCAGCCAC GTTTGACCCA GTTGATGTGG TTGCTGCCTG TAAGGAGTCC 
TTTGCAAAAT CCAAGAGTAC GTGGCTGTCA CCAGATGGAT TTGTATTTGC TGGATATAAG 
AGCAGCATTG AAAGCAACCT GCACCCTCGG ATGCCTGATG AGGCACGTGT GGAGGAGTTA 
CGAGAGAAAT GGCAGGAGAA TGCTCTGTTT GCTAACATGG CGCCAGTGCT GCCACGAGGC 
AGATGGAGCT GGGACAAACG GCATGTTGAC TTCGAGCTCT ACAAGAAGCC GCCTGAACTC 
CCCATGGCAA CAGCCACACT GACAGATGCT ACTCACTTTG TGCAAAATGT GTGTGATGAT 
ACCAAGCTGA AGGTTCACTG GTGTTCTGCA GCAGCTGAGT TACGCGCACG GGAGCCAGGA 
GCCACTTCTG AGCTACAGCA TCTCCAAGGA CTTCTGAAAG ATGAGCCTCT GAAATTCCCA 
CTCAGGAAGC CAGGACTGAT TTTGAAGCCT AACCCAGCCC TCTCAGTGTT GGACTCTCAG 
AACAGCTCAG ATGTCCTGCC AGGAAAGAAG AGCGTTTGCA GTGGCTTTGT GCCAGGCCTT 
CAAGACCACC ACAGCCGGAA GTGGAACAAG AACATTATCC CCTGGCACAA CAGAGAGCAT 
AAGAAGATTG AAAAACTTGA AGGCGCAGAT TTCAATTTAC TTGGCTCTAA ACAATCAGTT 
TACAAGAGTG ACCAAGCAGC AGGCAGACAC GCTACAACCT ATGCACAACA GGAAGACCCT 
CTCCAGAATG ATATTCTGGA TACAGCTACT GCATCACAGC GTTGGGATGA CCCCCTCAGT 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIAA1257 ( XM_004944522.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004944579.2 Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X6, mRNA.

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CloneID	OGa41714
Clone ID Related Accession (Same CDS sequence)	XM_025154698.1 , XM_004944522.2
Accession Version	XM_004944522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	uncharacterized protein KIAA1257 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455943.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004944522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG AAACAGATTT 
GAGGGTAACG CCCAACTTCT GAAACAGAAA GCAGTTGCTG CTCCTTCACA TAATGCAGCT 
TCAGAAGAAA CTGGGGAAAC AGCCAGCAAT GTGGGAATTC CGATGTGTCA AACAGACAAG 
ACAGCTACCT TTAATGGTAG AAGAGATTCT GGTGCTAAAA AAGGCATGGA TACTACTAGG 
GCAAACATTG CATCACTGCA GTTGGATCTC ATGCCTCTCC TCTCAGGAGA AAATTCAGTG 
ATCAGTCGAC TAGAAGAAAA CAGTCCTATT CTTTTAGATG CTTATGTGTC TTTCACTGTA 
GAAACGCCTC TTCTGTCTGA AAGACAAATA CGTGAATTGA ATCCACTGGT TATAAAGATC 
TGTTCAGCTA CCAGCCTCCC GAACACACCT ATACCAATAG AAGTGCTACA GAAATTGTGT 
GTTCCTACCT ACTGCAAATA CAAATTCCAC AATTTTCCAG CTCATCAGAC TCATGGACAA 
ATCCACGGAA CTCATGTCTA CTTTAAGGAT GTTAATGTAT TTCTAACAGG AAGAATGAAA 
CCTGGGGAGT TACAGAGGTG TCTTAGAGGT CCCCCTTTGG AGATTGAAGT TCATGATCGA 
GATAGAAAGA TTGAAGACAA CAAAAAAGAG CCATGTCTAT TTGGGGAGGA TCACACTGAT 
GAAAATCTAG GAGAGTTGAG ACCCCTCCCT TGCCAGTCTG CAATCTGTAA TTCTTTTGCA 
GAGGATGAAG TGTGGCATCC ACATGGGGTA GCTAAAGTAA GTCTAAGTGA TCTATTGTTG 
GGTAAAAAGT ACTTGACTGT TAGTGCACCC ATTCGTAGCT GTTCAACTCC AAATACAGCT 
GCTTTCAATA AAAAGAATAA AAGAAAGAAG ATAGGGAAGA AGCATCCACA GATCCTATTA 
CCCATGGGAC ATTATCTACA ATCAGACTCG CTTTTGAAAT TAAGAGTGGA AATAGCAGTG 
CCATTGAGAA TGCATGCAGA AATTGATGAT GCTCAAGTGA CGGATTGCCC GTACAGTTGT 
ATAGTCTACA TTTTTGACTA CAGTAATTCA TTTTTATTAC ATGATTTGGT GGAAGAGATT 
ACAGAAATCA ATGCGAAAGC CCTTCAGCTG GACTGTTATC CTCCACACTT GATCAGAACA 
TCTCTTGCTA CATTAAAACT AAAAAGCATG CTTGAGAAGG TTTCTGAGTT AGATGTTGTT 
ACTGGCTTTC ACTTACTGGA TGGAGCTACT CACCTCTTTG TCTTGGAGGG TTTAAAAGAC 
AAAGCCATCA AGAGACTCTG GGATAGGCAC TTTGAAAGAA CTTACAGACA TGAAGATGGA 
CAACTGGAAA TACTATATAA TTCACAACTG TCTTTCAGTC AGCGCCTGTA TACCGACCTG 
GAAGCAATCT TTTATCGTTT CCACCTCTGT AAGCCCCTCT TCACTATACT GAAACAGCCT 
CTGCTTTATG TCAGGGGAGC AGTTCCTTGG GCGTGTTTCC AAGCCTTGTC CAGGCTCAGT 
TACCTCTGTC ACAGTAAGAG GTTGAGGGAT GTCATTCAGG GGGATCTGCT GCCCTCAGCA 
GAAATGATCA CAGCGTTGAG TCGAGAGTAT GGAGTTCCAT TAGCTGATGA GGATCTCTTC 
ACTCAGAAAC CTCCTCTACT CTTATTTTCG TCTGATTATA CACCACCAGG AAATGTAAGC 
AGAGAGAAAA AGGCACGATA TTCTTCATTA GATCACTACA ACGAAAGATA TGTGCTGTGG 
AAGAAAGAAA TAGCAGACAA AAAGTCTTTT GAGAGAAACC ATATTAGGGC TAACATTGAT 
GCTGTGCGCC AGCTTAAAGG AAAGATGAAA AGGCTGAAGT TTGAAACTTT CAGGATTTCT 
CCTGCCGATG GCAAATCTGT TTATAACTAT AGCTCTCAGC GCCTGAATTC TGCAGAACTT 
GCAAAGAAGC ATCTTCGCCA GGAAATGGCA AAGGATCCAA AAACAAGGTT CAGTTATTGT 
CACGAGTATC TTTCAGCCAC GTTTGACCCA GTTGATGTGG TTGCTGCCTG TAAGGAGTCC 
TTTGCAAAAT CCAAGAGTAC GTGGCTGTCA CCAGATGGAT TTGTATTTGC TGGATATAAG 
AGCAGCATTG AAAGCAACCT GCACCCTCGG ATGCCTGATG AGGCACGTGT GGAGGAGTTA 
CGAGAGAAAT GGCAGGAGAA TGCTCTGTTT GCTAACATGG CGCCAGTGCT GCCACGAGGC 
AGATGGAGCT GGGACAAACG GCATGTTGAC TTCGAGCTCT ACAAGAAGCC GCCTGAACTC 
CCCATGGCAA CAGCCACACT GACAGATGCT ACTCACTTTG TGCAAAATGT GTGTGATGAT 
ACCAAGCTGA AGGTTCACTG GTGTTCTGCA GCAGCTGAGT TACGCGCACG GGAGCCAGGA 
GCCACTTCTG AGCTACAGCA TCTCCAAGGA CTTCTGAAAG ATGAGCCTCT GAAATTCCCA 
CTCAGGAAGC CAGGACTGAT TTTGAAGCCT AACCCAGCCC TCTCAGTGTT GGACTCTCAG 
AACAGCTCAG ATGTCCTGCC AGGAAAGAAG AGCGTTTGCA GTGGCTTTGT GCCAGGCCTT 
CAAGACCACC ACAGCCGGAA GTGGAACAAG AACATTATCC CCTGGCACAA CAGAGAGCAT 
AAGAAGATTG AAAAACTTGA AGGCGCAGAT TTCAATTTAC TTGGCTCTAA ACAATCAGTT 
TACAAGAGTG ACCAAGCAGC AGGCAGACAC GCTACAACCT ATGCACAACA GGAAGACCCT 
CTCCAGAATG ATATTCTGGA TACAGCTACT GCATCACAGC GTTGGGATGA CCCCCTCAGT 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004944579.2
CDS	2611..5859
Translation	MPWLENDQMWISWSHNTNVNVTNEFLVKLRDHKITLKLWDTKDQVFSEARLTKPDMSSIEGDAEAVDGVKHTVLQQRRLFEGSQPAPSCTKIREAKESKAQKESSALPIKEETDQSGLGTSSCNVLHPPKTDDSTVRNRFEGNAQLLKQKAVAAPSHNAASEETGETASNVGIPMCQTDKTATFNGRRDSGAKKGMDTTRANIASLQLDLMPLLSGENSVISRLEENSPILLDAYVSFTVETPLLSERQIRELNPLVIKICSATSLPNTPIPIEVLQKLCVPTYCKYKFHNFPAHQTHGQIHGTHVYFKDVNVFLTGRMKPGELQRCLRGPPLEIEVHDRDRKIEDNKKEPCLFGEDHTDENLGELRPLPCQSAICNSFAEDEVWHPHGVAKVSLSDLLLGKKYLTVSAPIRSCSTPNTAAFNKKNKRKKIGKKHPQILLPMGHYLQSDSLLKLRVEIAVPLRMHAEIDDAQVTDCPYSCIVYIFDYSNSFLLHDLVEEITEINAKALQLDCYPPHLIRTSLATLKLKSMLEKVSELDVVTGFHLLDGATHLFVLEGLKDKAIKRLWDRHFERTYRHEDGQLEILYNSQLSFSQRLYTDLEAIFYRFHLCKPLFTILKQPLLYVRGAVPWACFQALSRLSYLCHSKRLRDVIQGDLLPSAEMITALSREYGVPLADEDLFTQKPPLLLFSSDYTPPGNVSREKKARYSSLDHYNERYVLWKKEIADKKSFERNHIRANIDAVRQLKGKMKRLKFETFRISPADGKSVYNYSSQRLNSAELAKKHLRQEMAKDPKTRFSYCHEYLSATFDPVDVVAACKESFAKSKSTWLSPDGFVFAGYKSSIESNLHPRMPDEARVEELREKWQENALFANMAPVLPRGRWSWDKRHVDFELYKKPPELPMATATLTDATHFVQNVCDDTKLKVHWCSAAAELRAREPGATSELQHLQGLLKDEPLKFPLRKPGLILKPNPALSVLDSQNSSDVLPGKKSVCSGFVPGLQDHHSRKWNKNIIPWHNREHKKIEKLEGADFNLLGSKQSVYKSDQAAGRHATTYAQQEDPLQNDILDTATASQRWDDPLSGH

Target ORF information:

RefSeq Version	XM_004944522.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus uncharacterized LOC100857401 (LOC100857401), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004944522.2

ORF Insert Sequence:

ATGCCATGGC TTGAAAATGA CCAGATGTGG ATCTCGTGGA GCCATAATAC AAATGTCAAT 
GTCACTAATG AATTTCTAGT AAAATTAAGA GATCATAAAA TAACCTTAAA ACTTTGGGAC 
ACCAAGGACC AAGTTTTCTC AGAAGCAAGG CTTACTAAAC CAGACATGTC CTCAATAGAA 
GGCGATGCGG AGGCTGTTGA TGGAGTAAAA CACACAGTGC TGCAGCAAAG GAGATTGTTT 
GAAGGGAGTC AGCCAGCACC CAGCTGTACA AAAATTAGAG AAGCAAAGGA ATCCAAGGCA 
CAGAAAGAGT CTTCAGCCCT TCCAATCAAA GAGGAAACTG ATCAGAGTGG ACTGGGGACT 
TCCAGCTGTA ATGTACTACA TCCTCCAAAG ACGGATGACA GTACAGTAAG AAACAGATTT 
GAGGGTAACG CCCAACTTCT GAAACAGAAA GCAGTTGCTG CTCCTTCACA TAATGCAGCT 
TCAGAAGAAA CTGGGGAAAC AGCCAGCAAT GTGGGAATTC CGATGTGTCA AACAGACAAG 
ACAGCTACCT TTAATGGTAG AAGAGATTCT GGTGCTAAAA AAGGCATGGA TACTACTAGG 
GCAAACATTG CATCACTGCA GTTGGATCTC ATGCCTCTCC TCTCAGGAGA AAATTCAGTG 
ATCAGTCGAC TAGAAGAAAA CAGTCCTATT CTTTTAGATG CTTATGTGTC TTTCACTGTA 
GAAACGCCTC TTCTGTCTGA AAGACAAATA CGTGAATTGA ATCCACTGGT TATAAAGATC 
TGTTCAGCTA CCAGCCTCCC GAACACACCT ATACCAATAG AAGTGCTACA GAAATTGTGT 
GTTCCTACCT ACTGCAAATA CAAATTCCAC AATTTTCCAG CTCATCAGAC TCATGGACAA 
ATCCACGGAA CTCATGTCTA CTTTAAGGAT GTTAATGTAT TTCTAACAGG AAGAATGAAA 
CCTGGGGAGT TACAGAGGTG TCTTAGAGGT CCCCCTTTGG AGATTGAAGT TCATGATCGA 
GATAGAAAGA TTGAAGACAA CAAAAAAGAG CCATGTCTAT TTGGGGAGGA TCACACTGAT 
GAAAATCTAG GAGAGTTGAG ACCCCTCCCT TGCCAGTCTG CAATCTGTAA TTCTTTTGCA 
GAGGATGAAG TGTGGCATCC ACATGGGGTA GCTAAAGTAA GTCTAAGTGA TCTATTGTTG 
GGTAAAAAGT ACTTGACTGT TAGTGCACCC ATTCGTAGCT GTTCAACTCC AAATACAGCT 
GCTTTCAATA AAAAGAATAA AAGAAAGAAG ATAGGGAAGA AGCATCCACA GATCCTATTA 
CCCATGGGAC ATTATCTACA ATCAGACTCG CTTTTGAAAT TAAGAGTGGA AATAGCAGTG 
CCATTGAGAA TGCATGCAGA AATTGATGAT GCTCAAGTGA CGGATTGCCC GTACAGTTGT 
ATAGTCTACA TTTTTGACTA CAGTAATTCA TTTTTATTAC ATGATTTGGT GGAAGAGATT 
ACAGAAATCA ATGCGAAAGC CCTTCAGCTG GACTGTTATC CTCCACACTT GATCAGAACA 
TCTCTTGCTA CATTAAAACT AAAAAGCATG CTTGAGAAGG TTTCTGAGTT AGATGTTGTT 
ACTGGCTTTC ACTTACTGGA TGGAGCTACT CACCTCTTTG TCTTGGAGGG TTTAAAAGAC 
AAAGCCATCA AGAGACTCTG GGATAGGCAC TTTGAAAGAA CTTACAGACA TGAAGATGGA 
CAACTGGAAA TACTATATAA TTCACAACTG TCTTTCAGTC AGCGCCTGTA TACCGACCTG 
GAAGCAATCT TTTATCGTTT CCACCTCTGT AAGCCCCTCT TCACTATACT GAAACAGCCT 
CTGCTTTATG TCAGGGGAGC AGTTCCTTGG GCGTGTTTCC AAGCCTTGTC CAGGCTCAGT 
TACCTCTGTC ACAGTAAGAG GTTGAGGGAT GTCATTCAGG GGGATCTGCT GCCCTCAGCA 
GAAATGATCA CAGCGTTGAG TCGAGAGTAT GGAGTTCCAT TAGCTGATGA GGATCTCTTC 
ACTCAGAAAC CTCCTCTACT CTTATTTTCG TCTGATTATA CACCACCAGG AAATGTAAGC 
AGAGAGAAAA AGGCACGATA TTCTTCATTA GATCACTACA ACGAAAGATA TGTGCTGTGG 
AAGAAAGAAA TAGCAGACAA AAAGTCTTTT GAGAGAAACC ATATTAGGGC TAACATTGAT 
GCTGTGCGCC AGCTTAAAGG AAAGATGAAA AGGCTGAAGT TTGAAACTTT CAGGATTTCT 
CCTGCCGATG GCAAATCTGT TTATAACTAT AGCTCTCAGC GCCTGAATTC TGCAGAACTT 
GCAAAGAAGC ATCTTCGCCA GGAAATGGCA AAGGATCCAA AAACAAGGTT CAGTTATTGT 
CACGAGTATC TTTCAGCCAC GTTTGACCCA GTTGATGTGG TTGCTGCCTG TAAGGAGTCC 
TTTGCAAAAT CCAAGAGTAC GTGGCTGTCA CCAGATGGAT TTGTATTTGC TGGATATAAG 
AGCAGCATTG AAAGCAACCT GCACCCTCGG ATGCCTGATG AGGCACGTGT GGAGGAGTTA 
CGAGAGAAAT GGCAGGAGAA TGCTCTGTTT GCTAACATGG CGCCAGTGCT GCCACGAGGC 
AGATGGAGCT GGGACAAACG GCATGTTGAC TTCGAGCTCT ACAAGAAGCC GCCTGAACTC 
CCCATGGCAA CAGCCACACT GACAGATGCT ACTCACTTTG TGCAAAATGT GTGTGATGAT 
ACCAAGCTGA AGGTTCACTG GTGTTCTGCA GCAGCTGAGT TACGCGCACG GGAGCCAGGA 
GCCACTTCTG AGCTACAGCA TCTCCAAGGA CTTCTGAAAG ATGAGCCTCT GAAATTCCCA 
CTCAGGAAGC CAGGACTGAT TTTGAAGCCT AACCCAGCCC TCTCAGTGTT GGACTCTCAG 
AACAGCTCAG ATGTCCTGCC AGGAAAGAAG AGCGTTTGCA GTGGCTTTGT GCCAGGCCTT 
CAAGACCACC ACAGCCGGAA GTGGAACAAG AACATTATCC CCTGGCACAA CAGAGAGCAT 
AAGAAGATTG AAAAACTTGA AGGCGCAGAT TTCAATTTAC TTGGCTCTAA ACAATCAGTT 
TACAAGAGTG ACCAAGCAGC AGGCAGACAC GCTACAACCT ATGCACAACA GGAAGACCCT 
CTCCAGAATG ATATTCTGGA TACAGCTACT GCATCACAGC GTTGGGATGA CCCCCTCAGT 
GGACACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KIAA1257
Entrez Gene ID	100857401
Full Name	KIAA1257
General protein information	Preferred Names KIAA1257 Names uncharacterized protein KIAA1257 homolog
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Homo sapiens (human)	LOC100132731	XP_003403469.3
	LOC706563	XP_001094939.2
Gallus gallus (chicken)	LOC100857401	XP_004944576.1
Danio rerio (zebrafish)	LOC100334148	XP_002663836.2
Rattus norvegicus (Norway rat)	LOC100362908	XP_006236918.1
Canis lupus familiaris (dog)	LOC100683130	XP_005632144.1
Pan troglodytes (chimpanzee)	LOC100614863	XP_003310038.2
Bos taurus (cattle)	KIAA1257	XP_002707827.3
Xenopus tropicalis (tropical clawed frog)	LOC100485911	XP_002942847.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIAA1257 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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