PRIM2 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRIM2
Entrez Gene ID	103125782
Full Name	DNA primase subunit 2
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007536606.2	XP_007536668.1	DNA primase large subunit

Pathways from BioSystems
Gene Ontology

The following PRIM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRIM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr24673	XM_007536606.2 Latest version!	Erinaceus europaeus primase (DNA) subunit 2 (PRIM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRIM2 ( XM_007536606.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007536668.1 Erinaceus europaeus primase (DNA) subunit 2 (PRIM2), mRNA.

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CloneID	OEr24673
Clone ID Related Accession (Same CDS sequence)	XM_007536606.2
Accession Version	XM_007536606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	DNA primase large subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804879.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007536606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTTTT CTGGTGGAAA GCGGAGGAAA CTGAAGTTGG CAGGTGACCT GAGGAATTCT 
TGCTACCCAC ACAGTCTTCA GTTTTATTTG AAGCCTCCTT CTGAAAATAT ATCTTTGATG 
GAGTTTGAAA CCTTGGCTGT TGATAGACTT AAATTGCTGA AAGCAGTTGA AAATCTTGGT 
GTAAGTTATG TGAAAGGAAC AGAGCAATAC CAGACAAAAC TGGAGGCTGA GATTCGAAAG 
CTCAAGTTTT CCTATAGAGA AAATTTAGAA GATGAATATG AACCAAGAAG GAGGGATCAT 
ATTTCTCATT TTATTTTGCG CCTAGCATAT TGTCAATCGG AAGATCTTAG ACGTTGGTTC 
ATTCAACAAG AAATGGATCT CCTTCGATTT CGATTTAATA TTTTACCTAA GGACAAAATT 
CAAAATTTCT TAAAGGATAG TCATTTGCAC TTTGAGGCTA TAAGTGATGA AGAGAAGACT 
TTTCGAGAAC GGGAGATCAT TGCTTCATCA CCTAGTTTAA GTGGGACCAA GCTGGAGTTG 
GAATCTGTTT ATAAGATTCC TTTTGCTGAT GCCTTGGATT TATTCCGAAC CCGGAAAGTT 
TATTTGGAAG ATGGTTTTGC TTATGTGCCA CTTAGAGACA TTGTGGCAAT TATCCTAAAT 
GAATTTAGAG CCAAACTATC CAAGGCTTTG GCATTAACAG CCAGGTCCTT ACCTGCTGTG 
CAGTCTGATG AGAGACTTCA GCCTCTGCTT AAACACCTCA GTCATTCCTA CACTGGTCAA 
GATTACAGTG CACAGGGAAA TACTGGGAAG ATTTCCTTAG ATCAAATCGA CTCGCTTTCT 
ACCAAATCCT TCCCACCTTG CATGCGCCAG TTACACAAAG CCTTGCGGGA AAACCACCAT 
CTTCGTCATG GAGGCCGAAT GCAGTATGGC CTCTTCCTGA AGGGCATTGG CTTAACACTG 
GAACAGGCAT TGCAGTTCTG GAAACAAGAA TTTATCAGAG GAAACATGGA TGCAGACAAG 
TTTGATAAAT GCTACTCCTA TAACATACGT CATAGCTTTG GAAAGGAAGG CAAGAGGACA 
GACTATACAC CTTTCAGTTG CTTGAAGATC ATTGTAACCA ATCCACCAAG CCAAGGAGAT 
TATCATGGGT GCCCATTCCA TCATAGTGAT CCAGAGCTGC TGAAGCAGAA GTTGCAGTCA 
TACAAGATCT CTCCCGGAGG GATAAACCAG ATTTTGGATT TAGTAAAAGG AACACATTAC 
CAAGTAGCCT GTCAGAAGTA CTTTGAGATG ATTCATAATG TCGATGACTG TGGCTTTTCT 
TTGAGTCATC CTAATCAATT CTTCTTTGAG AGCCAGCGGA TTTTAAATGG TGGTAAAGAA 
ATGAAGAAGG AATCCATCCA CCCAGAAACT TCTCAACCCA AACCAAGTAT CCAGAAAAAC 
AAGGATGCAT CATCTGCTCT TGCCCCTTTA AATTCATCTC TGGATATTGA TATGGAGGGA 
TTAGAGAACT ATTTCAGTGA CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007536668.1
CDS	17..1540
Translation	MQFSGGKRRKLKLAGDLRNSCYPHSLQFYLKPPSENISLMEFETLAVDRLKLLKAVENLGVSYVKGTEQYQTKLEAEIRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEDLRRWFIQQEMDLLRFRFNILPKDKIQNFLKDSHLHFEAISDEEKTFREREIIASSPSLSGTKLELESVYKIPFADALDLFRTRKVYLEDGFAYVPLRDIVAIILNEFRAKLSKALALTARSLPAVQSDERLQPLLKHLSHSYTGQDYSAQGNTGKISLDQIDSLSTKSFPPCMRQLHKALRENHHLRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIRGNMDADKFDKCYSYNIRHSFGKEGKRTDYTPFSCLKIIVTNPPSQGDYHGCPFHHSDPELLKQKLQSYKISPGGINQILDLVKGTHYQVACQKYFEMIHNVDDCGFSLSHPNQFFFESQRILNGGKEMKKESIHPETSQPKPSIQKNKDASSALAPLNSSLDIDMEGLENYFSD

Target ORF information:

RefSeq Version	XM_007536606.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus primase (DNA) subunit 2 (PRIM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007536606.2

ORF Insert Sequence:

ATGCAGTTTT CTGGTGGAAA GCGGAGGAAA CTGAAGTTGG CAGGTGACCT GAGGAATTCT 
TGCTACCCAC ACAGTCTTCA GTTTTATTTG AAGCCTCCTT CTGAAAATAT ATCTTTGATG 
GAGTTTGAAA CCTTGGCTGT TGATAGACTT AAATTGCTGA AAGCAGTTGA AAATCTTGGT 
GTAAGTTATG TGAAAGGAAC AGAGCAATAC CAGACAAAAC TGGAGGCTGA GATTCGAAAG 
CTCAAGTTTT CCTATAGAGA AAATTTAGAA GATGAATATG AACCAAGAAG GAGGGATCAT 
ATTTCTCATT TTATTTTGCG CCTAGCATAT TGTCAATCGG AAGATCTTAG ACGTTGGTTC 
ATTCAACAAG AAATGGATCT CCTTCGATTT CGATTTAATA TTTTACCTAA GGACAAAATT 
CAAAATTTCT TAAAGGATAG TCATTTGCAC TTTGAGGCTA TAAGTGATGA AGAGAAGACT 
TTTCGAGAAC GGGAGATCAT TGCTTCATCA CCTAGTTTAA GTGGGACCAA GCTGGAGTTG 
GAATCTGTTT ATAAGATTCC TTTTGCTGAT GCCTTGGATT TATTCCGAAC CCGGAAAGTT 
TATTTGGAAG ATGGTTTTGC TTATGTGCCA CTTAGAGACA TTGTGGCAAT TATCCTAAAT 
GAATTTAGAG CCAAACTATC CAAGGCTTTG GCATTAACAG CCAGGTCCTT ACCTGCTGTG 
CAGTCTGATG AGAGACTTCA GCCTCTGCTT AAACACCTCA GTCATTCCTA CACTGGTCAA 
GATTACAGTG CACAGGGAAA TACTGGGAAG ATTTCCTTAG ATCAAATCGA CTCGCTTTCT 
ACCAAATCCT TCCCACCTTG CATGCGCCAG TTACACAAAG CCTTGCGGGA AAACCACCAT 
CTTCGTCATG GAGGCCGAAT GCAGTATGGC CTCTTCCTGA AGGGCATTGG CTTAACACTG 
GAACAGGCAT TGCAGTTCTG GAAACAAGAA TTTATCAGAG GAAACATGGA TGCAGACAAG 
TTTGATAAAT GCTACTCCTA TAACATACGT CATAGCTTTG GAAAGGAAGG CAAGAGGACA 
GACTATACAC CTTTCAGTTG CTTGAAGATC ATTGTAACCA ATCCACCAAG CCAAGGAGAT 
TATCATGGGT GCCCATTCCA TCATAGTGAT CCAGAGCTGC TGAAGCAGAA GTTGCAGTCA 
TACAAGATCT CTCCCGGAGG GATAAACCAG ATTTTGGATT TAGTAAAAGG AACACATTAC 
CAAGTAGCCT GTCAGAAGTA CTTTGAGATG ATTCATAATG TCGATGACTG TGGCTTTTCT 
TTGAGTCATC CTAATCAATT CTTCTTTGAG AGCCAGCGGA TTTTAAATGG TGGTAAAGAA 
ATGAAGAAGG AATCCATCCA CCCAGAAACT TCTCAACCCA AACCAAGTAT CCAGAAAAAC 
AAGGATGCAT CATCTGCTCT TGCCCCTTTA AATTCATCTC TGGATATTGA TATGGAGGGA 
TTAGAGAACT ATTTCAGTGA CTAA