VPS45 cDNA ORF clone, Erinaceus europaeus(western European hedgehog)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS45
Entrez Gene ID	103121065
Full Name	vacuolar protein sorting 45 homolog
Gene Type	protein-coding
Organism	Erinaceus europaeus(western European hedgehog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007531566.2	XP_007531628.1	vacuolar protein sorting-associated protein 45

Pathways from BioSystems
Gene Ontology

The following VPS45 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS45 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OEr19650	XM_007531566.2 Latest version!	Erinaceus europaeus vacuolar protein sorting 45 homolog (VPS45), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS45 ( XM_007531566.2 ) cDNA ORF clone, Erinaceus europaeus(western European hedgehog) -> XP_007531628.1 Erinaceus europaeus vacuolar protein sorting 45 homolog (VPS45), mRNA.

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CloneID	OEr19650
Clone ID Related Accession (Same CDS sequence)	XM_007531566.2
Accession Version	XM_007531566.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1713bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-10
Organism	Erinaceus europaeus(western European hedgehog)
Product	vacuolar protein sorting-associated protein 45
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006804461.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Apr 11, 2016 this sequence version replaced XM_007531566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Erinaceus europaeus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGTGG TTTTTGCTGT AAAGCAGTAC ATTTCCAAGA TGATAGAGGA CAGCGGGCCG 
GGCATGAAAG TTCTTCTCAT GGATAAAGAG ACGACTGGTA TAGTGAGTAT GGTGTATACA 
CAATCAGAGA TTCTTCAGAA GGAAGTCTAC CTTTTTGAAC GCATTGATTC TCAAAATAGA 
GAAATCATGA AACACTTGAA AGCAATTTGT TTTCTTCGTC CTACGAAGGA GAATGTCAAT 
TACCTGATTC AGGAACTTCG AAGACCCAAG TACAGTATAT ATTTTATTTA TTTCAGTAAT 
GTGATCAGCA AGAGTGATGT AAAGTCATTA GCTGAAGCTG ATGAACAGGA AGTTGTGGCG 
GAGGTTCAGG AATTTTATGG TGATTACATT GCTGTGAATC CACATTTGTT TTCCCTCAAT 
ATTTTGGGTT GCTGCCAGGG TCGAAATTGG GAGCCAGTGC AACTATCCAG AACAACTCAA 
GGATTGACAG CACTCCTTTT ATCTCTGAAG AAATGCCCCA TGATTCGTTA CCAGCTTTCA 
TCAGAGGCAG CAAAGAGACT TGCAGAATGT GTGAAGCAAG TGATAACTAA AGAATATGAA 
CTGTTTGAAT TCAGGCGAAC TGAAGTTCCT CCATTGCTGC TTATTTTAGA TCGCAGTGAT 
GATGCCATCA CCCCATTGCT AAACCAGTGG ACATATCAAG CCATGGTCCA TGAGCTACTG 
GGCATAAACA ACAATCGGAT TGATCTTTCC AGAGTGCCTG GGATAAGTAA AGACTTAAGA 
GAAGTTGTGT TATCTGCTGA AAATGATGAA TTCTATGCTA ATAATATGTA CCTGAACTTT 
GCTGAAATTG GTAGCAATAT AAAGAGTCTC ATGGAAGACT TTCAGAAGAA GAAACCGAAA 
GAACAACAAA AACTAGAATC AATAGCAGAC ATGAAGGCCT TTGTTGAAAA TTATCCACAG 
TTCAAGAAGA TGTCTGGAAC TGTATCAAAG CACGTGACAG TAGTTGGAGA GTTGTCTCGG 
TTGGTCAGTG AGCGGAATCT GCTGGAAGTG TCAGAAGTTG AGCAAGAACT GGCCTGTCAA 
AATGACCATT CTAATGCTCT CCAGAATGTG AAGAGGCTCC TGCAGAACTC CAAAGTGACA 
GAGTTTGATG CTGCCCGCCT GGTAATGCTT TATGCTCTAC GTTATGAGCG ACACAGCAGC 
AACAGCCTAC CAGGGCTCAT GATGGACCTC AGGAATAGGG GTGTTTCAGA GAAATACCGA 
AAGCTCGTGT CTGCAGTGGT TGAATATGGA GGTAAACGGG TCAGGGGAAG TGACCTCTTC 
AGCCCCAAAG ATGCAGTGTC TATTACCAAA CAGTTCCTCA AAGGACTGAA GGGAATAGAA 
AATGTGTATA CACAGCATCA ACCTTTCCTA CATGAGACCC TGGACCATCT TATCAAAGGA 
AAGCTTAAAG AAAATGTGTA CCCTTATCTA GGTCCCAGCA CACTCAGAGA CAGACCTCAG 
GATATCATTG TATTTGTAAT TGGAGGAGCC ACCTATGAAG AGGCTCTAAC AGTATATAAC 
CTGAACCATA ATACTCCTGG TGTGAGGATT GTCCTGGGAG GAACCACAGT GCATAACACA 
AAAAGTTTTC TAGAGGAAGT TTTGGCTTCT GGACTGCACA GCCGAAGCAA GGAGAGCTCG 
CAAGTGACAT CAAGGTCAGC AAGCAGAAGG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007531628.1
CDS	244..1956
Translation	MNVVFAVKQYISKMIEDSGPGMKVLLMDKETTGIVSMVYTQSEILQKEVYLFERIDSQNREIMKHLKAICFLRPTKENVNYLIQELRRPKYSIYFIYFSNVISKSDVKSLAEADEQEVVAEVQEFYGDYIAVNPHLFSLNILGCCQGRNWEPVQLSRTTQGLTALLLSLKKCPMIRYQLSSEAAKRLAECVKQVITKEYELFEFRRTEVPPLLLILDRSDDAITPLLNQWTYQAMVHELLGINNNRIDLSRVPGISKDLREVVLSAENDEFYANNMYLNFAEIGSNIKSLMEDFQKKKPKEQQKLESIADMKAFVENYPQFKKMSGTVSKHVTVVGELSRLVSERNLLEVSEVEQELACQNDHSNALQNVKRLLQNSKVTEFDAARLVMLYALRYERHSSNSLPGLMMDLRNRGVSEKYRKLVSAVVEYGGKRVRGSDLFSPKDAVSITKQFLKGLKGIENVYTQHQPFLHETLDHLIKGKLKENVYPYLGPSTLRDRPQDIIVFVIGGATYEEALTVYNLNHNTPGVRIVLGGTTVHNTKSFLEEVLASGLHSRSKESSQVTSRSASRR

Target ORF information:

RefSeq Version	XM_007531566.2
Organism	Erinaceus europaeus(western European hedgehog)
Definition	Erinaceus europaeus vacuolar protein sorting 45 homolog (VPS45), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007531566.2

ORF Insert Sequence:

ATGAACGTGG TTTTTGCTGT AAAGCAGTAC ATTTCCAAGA TGATAGAGGA CAGCGGGCCG 
GGCATGAAAG TTCTTCTCAT GGATAAAGAG ACGACTGGTA TAGTGAGTAT GGTGTATACA 
CAATCAGAGA TTCTTCAGAA GGAAGTCTAC CTTTTTGAAC GCATTGATTC TCAAAATAGA 
GAAATCATGA AACACTTGAA AGCAATTTGT TTTCTTCGTC CTACGAAGGA GAATGTCAAT 
TACCTGATTC AGGAACTTCG AAGACCCAAG TACAGTATAT ATTTTATTTA TTTCAGTAAT 
GTGATCAGCA AGAGTGATGT AAAGTCATTA GCTGAAGCTG ATGAACAGGA AGTTGTGGCG 
GAGGTTCAGG AATTTTATGG TGATTACATT GCTGTGAATC CACATTTGTT TTCCCTCAAT 
ATTTTGGGTT GCTGCCAGGG TCGAAATTGG GAGCCAGTGC AACTATCCAG AACAACTCAA 
GGATTGACAG CACTCCTTTT ATCTCTGAAG AAATGCCCCA TGATTCGTTA CCAGCTTTCA 
TCAGAGGCAG CAAAGAGACT TGCAGAATGT GTGAAGCAAG TGATAACTAA AGAATATGAA 
CTGTTTGAAT TCAGGCGAAC TGAAGTTCCT CCATTGCTGC TTATTTTAGA TCGCAGTGAT 
GATGCCATCA CCCCATTGCT AAACCAGTGG ACATATCAAG CCATGGTCCA TGAGCTACTG 
GGCATAAACA ACAATCGGAT TGATCTTTCC AGAGTGCCTG GGATAAGTAA AGACTTAAGA 
GAAGTTGTGT TATCTGCTGA AAATGATGAA TTCTATGCTA ATAATATGTA CCTGAACTTT 
GCTGAAATTG GTAGCAATAT AAAGAGTCTC ATGGAAGACT TTCAGAAGAA GAAACCGAAA 
GAACAACAAA AACTAGAATC AATAGCAGAC ATGAAGGCCT TTGTTGAAAA TTATCCACAG 
TTCAAGAAGA TGTCTGGAAC TGTATCAAAG CACGTGACAG TAGTTGGAGA GTTGTCTCGG 
TTGGTCAGTG AGCGGAATCT GCTGGAAGTG TCAGAAGTTG AGCAAGAACT GGCCTGTCAA 
AATGACCATT CTAATGCTCT CCAGAATGTG AAGAGGCTCC TGCAGAACTC CAAAGTGACA 
GAGTTTGATG CTGCCCGCCT GGTAATGCTT TATGCTCTAC GTTATGAGCG ACACAGCAGC 
AACAGCCTAC CAGGGCTCAT GATGGACCTC AGGAATAGGG GTGTTTCAGA GAAATACCGA 
AAGCTCGTGT CTGCAGTGGT TGAATATGGA GGTAAACGGG TCAGGGGAAG TGACCTCTTC 
AGCCCCAAAG ATGCAGTGTC TATTACCAAA CAGTTCCTCA AAGGACTGAA GGGAATAGAA 
AATGTGTATA CACAGCATCA ACCTTTCCTA CATGAGACCC TGGACCATCT TATCAAAGGA 
AAGCTTAAAG AAAATGTGTA CCCTTATCTA GGTCCCAGCA CACTCAGAGA CAGACCTCAG 
GATATCATTG TATTTGTAAT TGGAGGAGCC ACCTATGAAG AGGCTCTAAC AGTATATAAC 
CTGAACCATA ATACTCCTGG TGTGAGGATT GTCCTGGGAG GAACCACAGT GCATAACACA 
AAAAGTTTTC TAGAGGAAGT TTTGGCTTCT GGACTGCACA GCCGAAGCAA GGAGAGCTCG 
CAAGTGACAT CAAGGTCAGC AAGCAGAAGG TGA