RFWD3 cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFWD3
Entrez Gene ID	100055114
Full Name	ring finger and WD repeat domain 3
General protein information	Preferred Names ring finger and WD repeat domain 3 Names E3 ubiquitin-protein ligase RFWD3
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023637327.1	XP_023493095.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_005608428.2	XP_005608485.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_023637324.1	XP_023493092.1	E3 ubiquitin-protein ligase RFWD3 isoform X1
XM_023637325.1	XP_023493093.1	E3 ubiquitin-protein ligase RFWD3 isoform X2
XM_005608427.2	XP_005608484.1	E3 ubiquitin-protein ligase RFWD3 isoform X1

Pathways from BioSystems
Gene Ontology

The following RFWD3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFWD3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHb18462	XM_023637324.1 Latest version!	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHb18462	XM_005608427.2 Latest version!	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHb18462	XM_023637327.1 Latest version!	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHb18463	XM_023637325.1 Latest version!	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHb18463	XM_005608428.2 Latest version!	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFWD3 ( XM_023637327.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023493095.1 Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHb18462
Clone ID Related Accession (Same CDS sequence)	XM_023637327.1 , XM_023637324.1 , XM_005608427.2
Accession Version	XM_023637327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009146.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023493095.1
CDS	159..2786
Translation	MAHEAMEYDLHVQLDHLAADQPAPAQVVSSQGGPLLLQPAPAQVVSQGAPLLLQPAPAQVVSSQGGPLLLRPAPVQVVSSQGGPLLLRPAPVQVVSGQGGPLLLRPAPAQVVSGQGGPLLLRPAPAQVVSSQGGPLLLQPAPAQVVSSQGGPLLLQPAPADVVSSQRGPPLLQPAPQVSIDLTEEVELLGEGSVENVNPGPSEEHTQESGANRTLGVSSLNSVNSFISGLQRLHGMLQFLRPPSDSGVGPIRARRRRSASHRTRVGGSQRTDNARSRAPLDAYFQVSRTQPHLPATSNDSETRNPVSADLQVSSRSDSDSDSSTENEEVVVLAEEPGAVVLEEQVGGMSAEQEVTCINGEETLPKQQSPQKSSPLVPSVSMDEEEGDTCTICLEQWTNVGDHRLSALRCGHLFGYGCISKWLKGQARKCPQCNKKAKQSDIVVLYARTLKALDTSEHEHMKSSLLKEQMLRQQAELESAQCRLQLQVLTEECTRLHSRVQDLQKLIVQHQDQISQQPRGSQARLQNSVPSSQGQHKYHFQKTFTVSQTGNCRIMTYCDALGCLVVSQPSPQASFLPGFGVKMLSTANMKSSQYIPMHSKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYMYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASATFPYGGVLAGTLENASFWELKMGFSHWPHVLPVEPGGCIDFQTESSTRHCLVTYRPDKNHTTLRSVLMEMSYKLDDAGEPVCSCHPVQTFLGGPTCKLLTKSAIFQNPKNDGNILVCTGDEASNSALLWDAGSGSLLQDLQTHQPVLDICPFEVNHSSYLATLTEKMVHLYRWE

Target ORF information:

RefSeq Version	XM_023637327.1
Organism	Equus caballus(horse)
Definition	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023637327.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_023637324.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023493092.1 Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHb18462
Clone ID Related Accession (Same CDS sequence)	XM_023637327.1 , XM_023637324.1 , XM_005608427.2
Accession Version	XM_023637324.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009146.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023493092.1
CDS	22..2649
Translation	MAHEAMEYDLHVQLDHLAADQPAPAQVVSSQGGPLLLQPAPAQVVSQGAPLLLQPAPAQVVSSQGGPLLLRPAPVQVVSSQGGPLLLRPAPVQVVSGQGGPLLLRPAPAQVVSGQGGPLLLRPAPAQVVSSQGGPLLLQPAPAQVVSSQGGPLLLQPAPADVVSSQRGPPLLQPAPQVSIDLTEEVELLGEGSVENVNPGPSEEHTQESGANRTLGVSSLNSVNSFISGLQRLHGMLQFLRPPSDSGVGPIRARRRRSASHRTRVGGSQRTDNARSRAPLDAYFQVSRTQPHLPATSNDSETRNPVSADLQVSSRSDSDSDSSTENEEVVVLAEEPGAVVLEEQVGGMSAEQEVTCINGEETLPKQQSPQKSSPLVPSVSMDEEEGDTCTICLEQWTNVGDHRLSALRCGHLFGYGCISKWLKGQARKCPQCNKKAKQSDIVVLYARTLKALDTSEHEHMKSSLLKEQMLRQQAELESAQCRLQLQVLTEECTRLHSRVQDLQKLIVQHQDQISQQPRGSQARLQNSVPSSQGQHKYHFQKTFTVSQTGNCRIMTYCDALGCLVVSQPSPQASFLPGFGVKMLSTANMKSSQYIPMHSKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYMYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASATFPYGGVLAGTLENASFWELKMGFSHWPHVLPVEPGGCIDFQTESSTRHCLVTYRPDKNHTTLRSVLMEMSYKLDDAGEPVCSCHPVQTFLGGPTCKLLTKSAIFQNPKNDGNILVCTGDEASNSALLWDAGSGSLLQDLQTHQPVLDICPFEVNHSSYLATLTEKMVHLYRWE

Target ORF information:

RefSeq Version	XM_023637324.1
Organism	Equus caballus(horse)
Definition	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023637324.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005608427.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005608484.1 Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

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CloneID	OHb18462
Clone ID Related Accession (Same CDS sequence)	XM_023637327.1 , XM_023637324.1 , XM_005608427.2
Accession Version	XM_005608427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2628bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867410.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005608427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005608484.1
CDS	7..2634
Translation	MAHEAMEYDLHVQLDHLAADQPAPAQVVSSQGGPLLLQPAPAQVVSQGAPLLLQPAPAQVVSSQGGPLLLRPAPVQVVSSQGGPLLLRPAPVQVVSGQGGPLLLRPAPAQVVSGQGGPLLLRPAPAQVVSSQGGPLLLQPAPAQVVSSQGGPLLLQPAPADVVSSQRGPPLLQPAPQVSIDLTEEVELLGEGSVENVNPGPSEEHTQESGANRTLGVSSLNSVNSFISGLQRLHGMLQFLRPPSDSGVGPIRARRRRSASHRTRVGGSQRTDNARSRAPLDAYFQVSRTQPHLPATSNDSETRNPVSADLQVSSRSDSDSDSSTENEEVVVLAEEPGAVVLEEQVGGMSAEQEVTCINGEETLPKQQSPQKSSPLVPSVSMDEEEGDTCTICLEQWTNVGDHRLSALRCGHLFGYGCISKWLKGQARKCPQCNKKAKQSDIVVLYARTLKALDTSEHEHMKSSLLKEQMLRQQAELESAQCRLQLQVLTEECTRLHSRVQDLQKLIVQHQDQISQQPRGSQARLQNSVPSSQGQHKYHFQKTFTVSQTGNCRIMTYCDALGCLVVSQPSPQASFLPGFGVKMLSTANMKSSQYIPMHSKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYMYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASATFPYGGVLAGTLENASFWELKMGFSHWPHVLPVEPGGCIDFQTESSTRHCLVTYRPDKNHTTLRSVLMEMSYKLDDAGEPVCSCHPVQTFLGGPTCKLLTKSAIFQNPKNDGNILVCTGDEASNSALLWDAGSGSLLQDLQTHQPVLDICPFEVNHSSYLATLTEKMVHLYRWE

Target ORF information:

RefSeq Version	XM_005608427.2
Organism	Equus caballus(horse)
Definition	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005608427.2

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGCA GTCTCCCCAG AAGTCCAGCC CCCTTGTACC TTCTGTTTCT 
ATGGATGAGG AAGAAGGGGA CACCTGCACA ATATGTTTGG AACAGTGGAC CAATGTTGGG 
GATCACCGAC TCTCAGCATT ACGCTGTGGA CACCTCTTTG GATATGGGTG CATTTCTAAG 
TGGCTTAAAG GACAGGCTCG AAAATGTCCC CAGTGCAACA AGAAAGCCAA GCAAAGTGAC 
ATCGTTGTCC TTTACGCCCG AACCTTGAAA GCTTTGGACA CTAGTGAACA TGAGCACATG 
AAAAGTTCCT TACTGAAGGA GCAGATGCTG CGGCAACAGG CCGAGCTAGA GTCAGCACAG 
TGCCGGCTCC AGCTGCAAGT CCTCACTGAG GAGTGCACCA GGCTTCATAG TCGTGTCCAG 
GACTTGCAAA AACTTATTGT GCAGCATCAG GATCAGATTT CACAGCAACC CAGGGGCTCC 
CAAGCACGTT TACAAAACAG CGTGCCCTCC AGCCAGGGCC AGCACAAGTA CCATTTCCAG 
AAGACCTTCA CGGTGTCTCA GACAGGAAAC TGCCGAATTA TGACATACTG TGATGCTCTG 
GGCTGCCTGG TGGTGTCACA ACCTTCTCCT CAGGCCTCCT TCCTTCCAGG CTTTGGCGTT 
AAGATGTTGA GTACTGCCAA CATGAAGAGC AGTCAGTACA TTCCCATGCA TAGCAAACAG 
ATCCGCGGAC TGGCTTTCAG CAGTCGGTCC AAAGGCTTGC TGCTCTCTGC TTCTCTGGAC 
AGCACTATTA AACTGACCAG CCTGGAAACA AATACCGTGG TCCAGACTTA TAACGCTGGA 
CGTCCTGTCT GGAGCTGCTG CTGGTGTCTT GATGAAAACA ATTACATGTA TGCCGGACTG 
GTCAATGGTT CAATTCTGGT GTATGACCTT CGAAACACGA GCAGTCATAT CCAGGAGTTA 
GTACCTCAGA AAGCCAGATG CCCACTGGTA TCCTTGTCAT ACATACCCCG AGCTGCCTCA 
GCTACATTTC CGTATGGTGG AGTGTTGGCT GGAACCTTGG AGAACGCTTC CTTCTGGGAA 
CTGAAAATGG GCTTTTCTCA TTGGCCTCAT GTGCTGCCTG TGGAGCCAGG GGGCTGCATA 
GACTTTCAGA CAGAGAGCAG TACCCGCCAC TGTCTTGTGA CCTACAGGCC TGATAAAAAT 
CATACCACCT TACGAAGCGT GCTGATGGAG ATGTCCTACA AGCTGGATGA TGCTGGAGAG 
CCAGTTTGCT CCTGCCATCC TGTACAAACA TTCCTTGGGG GACCCACTTG CAAACTGCTA 
ACCAAAAGTG CCATTTTCCA AAACCCAAAG AATGATGGCA ACATCCTGGT GTGTACTGGG 
GATGAAGCGT CAAATTCTGC CCTGCTGTGG GATGCTGGGA GTGGCTCCTT GCTACAGGAT 
CTGCAGACCC ATCAGCCTGT CTTGGACATC TGCCCATTTG AGGTGAACCA TAGCAGCTAC 
TTGGCTACCT TAACAGAGAA GATGGTCCAC CTCTATAGGT GGGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_005608428.2 ) cDNA ORF clone, Equus caballus(horse) -> XP_005608485.1 Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHb18463
Clone ID Related Accession (Same CDS sequence)	XM_005608428.2 , XM_023637325.1
Accession Version	XM_005608428.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867410.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_005608428.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGTC TCCCCAGAAG TCCAGCCCCC TTGTACCTTC TGTTTCTATG 
GATGAGGAAG AAGGGGACAC CTGCACAATA TGTTTGGAAC AGTGGACCAA TGTTGGGGAT 
CACCGACTCT CAGCATTACG CTGTGGACAC CTCTTTGGAT ATGGGTGCAT TTCTAAGTGG 
CTTAAAGGAC AGGCTCGAAA ATGTCCCCAG TGCAACAAGA AAGCCAAGCA AAGTGACATC 
GTTGTCCTTT ACGCCCGAAC CTTGAAAGCT TTGGACACTA GTGAACATGA GCACATGAAA 
AGTTCCTTAC TGAAGGAGCA GATGCTGCGG CAACAGGCCG AGCTAGAGTC AGCACAGTGC 
CGGCTCCAGC TGCAAGTCCT CACTGAGGAG TGCACCAGGC TTCATAGTCG TGTCCAGGAC 
TTGCAAAAAC TTATTGTGCA GCATCAGGAT CAGATTTCAC AGCAACCCAG GGGCTCCCAA 
GCACGTTTAC AAAACAGCGT GCCCTCCAGC CAGGGCCAGC ACAAGTACCA TTTCCAGAAG 
ACCTTCACGG TGTCTCAGAC AGGAAACTGC CGAATTATGA CATACTGTGA TGCTCTGGGC 
TGCCTGGTGG TGTCACAACC TTCTCCTCAG GCCTCCTTCC TTCCAGGCTT TGGCGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CCATGCATAG CAAACAGATC 
CGCGGACTGG CTTTCAGCAG TCGGTCCAAA GGCTTGCTGC TCTCTGCTTC TCTGGACAGC 
ACTATTAAAC TGACCAGCCT GGAAACAAAT ACCGTGGTCC AGACTTATAA CGCTGGACGT 
CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT GAAAACAATT ACATGTATGC CGGACTGGTC 
AATGGTTCAA TTCTGGTGTA TGACCTTCGA AACACGAGCA GTCATATCCA GGAGTTAGTA 
CCTCAGAAAG CCAGATGCCC ACTGGTATCC TTGTCATACA TACCCCGAGC TGCCTCAGCT 
ACATTTCCGT ATGGTGGAGT GTTGGCTGGA ACCTTGGAGA ACGCTTCCTT CTGGGAACTG 
AAAATGGGCT TTTCTCATTG GCCTCATGTG CTGCCTGTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAGCAGTAC CCGCCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAT 
ACCACCTTAC GAAGCGTGCT GATGGAGATG TCCTACAAGC TGGATGATGC TGGAGAGCCA 
GTTTGCTCCT GCCATCCTGT ACAAACATTC CTTGGGGGAC CCACTTGCAA ACTGCTAACC 
AAAAGTGCCA TTTTCCAAAA CCCAAAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCGTCAA ATTCTGCCCT GCTGTGGGAT GCTGGGAGTG GCTCCTTGCT ACAGGATCTG 
CAGACCCATC AGCCTGTCTT GGACATCTGC CCATTTGAGG TGAACCATAG CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACCTC TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005608485.1
CDS	7..2631
Translation	MAHEAMEYDLHVQLDHLAADQPAPAQVVSSQGGPLLLQPAPAQVVSQGAPLLLQPAPAQVVSSQGGPLLLRPAPVQVVSSQGGPLLLRPAPVQVVSGQGGPLLLRPAPAQVVSGQGGPLLLRPAPAQVVSSQGGPLLLQPAPAQVVSSQGGPLLLQPAPADVVSSQRGPPLLQPAPQVSIDLTEEVELLGEGSVENVNPGPSEEHTQESGANRTLGVSSLNSVNSFISGLQRLHGMLQFLRPPSDSGVGPIRARRRRSASHRTRVGGSQRTDNARSRAPLDAYFQVSRTQPHLPATSNDSETRNPVSADLQVSSRSDSDSDSSTENEEVVVLAEEPGAVVLEEQVGGMSAEQEVTCINGEETLPKQSPQKSSPLVPSVSMDEEEGDTCTICLEQWTNVGDHRLSALRCGHLFGYGCISKWLKGQARKCPQCNKKAKQSDIVVLYARTLKALDTSEHEHMKSSLLKEQMLRQQAELESAQCRLQLQVLTEECTRLHSRVQDLQKLIVQHQDQISQQPRGSQARLQNSVPSSQGQHKYHFQKTFTVSQTGNCRIMTYCDALGCLVVSQPSPQASFLPGFGVKMLSTANMKSSQYIPMHSKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYMYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASATFPYGGVLAGTLENASFWELKMGFSHWPHVLPVEPGGCIDFQTESSTRHCLVTYRPDKNHTTLRSVLMEMSYKLDDAGEPVCSCHPVQTFLGGPTCKLLTKSAIFQNPKNDGNILVCTGDEASNSALLWDAGSGSLLQDLQTHQPVLDICPFEVNHSSYLATLTEKMVHLYRWE

Target ORF information:

RefSeq Version	XM_005608428.2
Organism	Equus caballus(horse)
Definition	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005608428.2

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGTC TCCCCAGAAG TCCAGCCCCC TTGTACCTTC TGTTTCTATG 
GATGAGGAAG AAGGGGACAC CTGCACAATA TGTTTGGAAC AGTGGACCAA TGTTGGGGAT 
CACCGACTCT CAGCATTACG CTGTGGACAC CTCTTTGGAT ATGGGTGCAT TTCTAAGTGG 
CTTAAAGGAC AGGCTCGAAA ATGTCCCCAG TGCAACAAGA AAGCCAAGCA AAGTGACATC 
GTTGTCCTTT ACGCCCGAAC CTTGAAAGCT TTGGACACTA GTGAACATGA GCACATGAAA 
AGTTCCTTAC TGAAGGAGCA GATGCTGCGG CAACAGGCCG AGCTAGAGTC AGCACAGTGC 
CGGCTCCAGC TGCAAGTCCT CACTGAGGAG TGCACCAGGC TTCATAGTCG TGTCCAGGAC 
TTGCAAAAAC TTATTGTGCA GCATCAGGAT CAGATTTCAC AGCAACCCAG GGGCTCCCAA 
GCACGTTTAC AAAACAGCGT GCCCTCCAGC CAGGGCCAGC ACAAGTACCA TTTCCAGAAG 
ACCTTCACGG TGTCTCAGAC AGGAAACTGC CGAATTATGA CATACTGTGA TGCTCTGGGC 
TGCCTGGTGG TGTCACAACC TTCTCCTCAG GCCTCCTTCC TTCCAGGCTT TGGCGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CCATGCATAG CAAACAGATC 
CGCGGACTGG CTTTCAGCAG TCGGTCCAAA GGCTTGCTGC TCTCTGCTTC TCTGGACAGC 
ACTATTAAAC TGACCAGCCT GGAAACAAAT ACCGTGGTCC AGACTTATAA CGCTGGACGT 
CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT GAAAACAATT ACATGTATGC CGGACTGGTC 
AATGGTTCAA TTCTGGTGTA TGACCTTCGA AACACGAGCA GTCATATCCA GGAGTTAGTA 
CCTCAGAAAG CCAGATGCCC ACTGGTATCC TTGTCATACA TACCCCGAGC TGCCTCAGCT 
ACATTTCCGT ATGGTGGAGT GTTGGCTGGA ACCTTGGAGA ACGCTTCCTT CTGGGAACTG 
AAAATGGGCT TTTCTCATTG GCCTCATGTG CTGCCTGTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAGCAGTAC CCGCCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAT 
ACCACCTTAC GAAGCGTGCT GATGGAGATG TCCTACAAGC TGGATGATGC TGGAGAGCCA 
GTTTGCTCCT GCCATCCTGT ACAAACATTC CTTGGGGGAC CCACTTGCAA ACTGCTAACC 
AAAAGTGCCA TTTTCCAAAA CCCAAAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCGTCAA ATTCTGCCCT GCTGTGGGAT GCTGGGAGTG GCTCCTTGCT ACAGGATCTG 
CAGACCCATC AGCCTGTCTT GGACATCTGC CCATTTGAGG TGAACCATAG CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACCTC TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFWD3 ( XM_023637325.1 ) cDNA ORF clone, Equus caballus(horse) -> XP_023493093.1 Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

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CloneID	OHb18463
Clone ID Related Accession (Same CDS sequence)	XM_005608428.2 , XM_023637325.1
Accession Version	XM_023637325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	E3 ubiquitin-protein ligase RFWD3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009146.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGTC TCCCCAGAAG TCCAGCCCCC TTGTACCTTC TGTTTCTATG 
GATGAGGAAG AAGGGGACAC CTGCACAATA TGTTTGGAAC AGTGGACCAA TGTTGGGGAT 
CACCGACTCT CAGCATTACG CTGTGGACAC CTCTTTGGAT ATGGGTGCAT TTCTAAGTGG 
CTTAAAGGAC AGGCTCGAAA ATGTCCCCAG TGCAACAAGA AAGCCAAGCA AAGTGACATC 
GTTGTCCTTT ACGCCCGAAC CTTGAAAGCT TTGGACACTA GTGAACATGA GCACATGAAA 
AGTTCCTTAC TGAAGGAGCA GATGCTGCGG CAACAGGCCG AGCTAGAGTC AGCACAGTGC 
CGGCTCCAGC TGCAAGTCCT CACTGAGGAG TGCACCAGGC TTCATAGTCG TGTCCAGGAC 
TTGCAAAAAC TTATTGTGCA GCATCAGGAT CAGATTTCAC AGCAACCCAG GGGCTCCCAA 
GCACGTTTAC AAAACAGCGT GCCCTCCAGC CAGGGCCAGC ACAAGTACCA TTTCCAGAAG 
ACCTTCACGG TGTCTCAGAC AGGAAACTGC CGAATTATGA CATACTGTGA TGCTCTGGGC 
TGCCTGGTGG TGTCACAACC TTCTCCTCAG GCCTCCTTCC TTCCAGGCTT TGGCGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CCATGCATAG CAAACAGATC 
CGCGGACTGG CTTTCAGCAG TCGGTCCAAA GGCTTGCTGC TCTCTGCTTC TCTGGACAGC 
ACTATTAAAC TGACCAGCCT GGAAACAAAT ACCGTGGTCC AGACTTATAA CGCTGGACGT 
CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT GAAAACAATT ACATGTATGC CGGACTGGTC 
AATGGTTCAA TTCTGGTGTA TGACCTTCGA AACACGAGCA GTCATATCCA GGAGTTAGTA 
CCTCAGAAAG CCAGATGCCC ACTGGTATCC TTGTCATACA TACCCCGAGC TGCCTCAGCT 
ACATTTCCGT ATGGTGGAGT GTTGGCTGGA ACCTTGGAGA ACGCTTCCTT CTGGGAACTG 
AAAATGGGCT TTTCTCATTG GCCTCATGTG CTGCCTGTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAGCAGTAC CCGCCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAT 
ACCACCTTAC GAAGCGTGCT GATGGAGATG TCCTACAAGC TGGATGATGC TGGAGAGCCA 
GTTTGCTCCT GCCATCCTGT ACAAACATTC CTTGGGGGAC CCACTTGCAA ACTGCTAACC 
AAAAGTGCCA TTTTCCAAAA CCCAAAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCGTCAA ATTCTGCCCT GCTGTGGGAT GCTGGGAGTG GCTCCTTGCT ACAGGATCTG 
CAGACCCATC AGCCTGTCTT GGACATCTGC CCATTTGAGG TGAACCATAG CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACCTC TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023493093.1
CDS	22..2646
Translation	MAHEAMEYDLHVQLDHLAADQPAPAQVVSSQGGPLLLQPAPAQVVSQGAPLLLQPAPAQVVSSQGGPLLLRPAPVQVVSSQGGPLLLRPAPVQVVSGQGGPLLLRPAPAQVVSGQGGPLLLRPAPAQVVSSQGGPLLLQPAPAQVVSSQGGPLLLQPAPADVVSSQRGPPLLQPAPQVSIDLTEEVELLGEGSVENVNPGPSEEHTQESGANRTLGVSSLNSVNSFISGLQRLHGMLQFLRPPSDSGVGPIRARRRRSASHRTRVGGSQRTDNARSRAPLDAYFQVSRTQPHLPATSNDSETRNPVSADLQVSSRSDSDSDSSTENEEVVVLAEEPGAVVLEEQVGGMSAEQEVTCINGEETLPKQSPQKSSPLVPSVSMDEEEGDTCTICLEQWTNVGDHRLSALRCGHLFGYGCISKWLKGQARKCPQCNKKAKQSDIVVLYARTLKALDTSEHEHMKSSLLKEQMLRQQAELESAQCRLQLQVLTEECTRLHSRVQDLQKLIVQHQDQISQQPRGSQARLQNSVPSSQGQHKYHFQKTFTVSQTGNCRIMTYCDALGCLVVSQPSPQASFLPGFGVKMLSTANMKSSQYIPMHSKQIRGLAFSSRSKGLLLSASLDSTIKLTSLETNTVVQTYNAGRPVWSCCWCLDENNYMYAGLVNGSILVYDLRNTSSHIQELVPQKARCPLVSLSYIPRAASATFPYGGVLAGTLENASFWELKMGFSHWPHVLPVEPGGCIDFQTESSTRHCLVTYRPDKNHTTLRSVLMEMSYKLDDAGEPVCSCHPVQTFLGGPTCKLLTKSAIFQNPKNDGNILVCTGDEASNSALLWDAGSGSLLQDLQTHQPVLDICPFEVNHSSYLATLTEKMVHLYRWE

Target ORF information:

RefSeq Version	XM_023637325.1
Organism	Equus caballus(horse)
Definition	Equus caballus ring finger and WD repeat domain 3 (RFWD3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023637325.1

ORF Insert Sequence:

ATGGCTCATG AAGCAATGGA ATATGATCTT CACGTGCAGT TAGATCATCT TGCTGCAGAT 
CAGCCTGCTC CTGCTCAAGT GGTCAGCAGC CAAGGAGGGC CACTCCTGCT TCAGCCTGCT 
CCGGCTCAGG TGGTCAGCCA AGGAGCTCCA CTCCTGCTTC AGCCTGCTCC TGCTCAGGTG 
GTCAGCAGCC AAGGAGGGCC ACTGCTGCTT CGGCCTGCTC CTGTTCAGGT GGTCAGCAGC 
CAAGGAGGGC CACTCCTGCT TCGGCCTGCT CCTGTTCAGG TGGTCAGCGG CCAAGGAGGG 
CCACTCCTGC TTCGGCCTGC TCCTGCTCAA GTGGTCAGCG GCCAAGGAGG GCCACTCCTG 
CTTCGGCCTG CTCCTGCTCA AGTGGTCAGC AGCCAAGGAG GGCCACTCCT GCTTCAGCCT 
GCTCCTGCTC AAGTGGTCAG CAGCCAAGGA GGGCCACTCC TGCTTCAGCC TGCACCTGCT 
GATGTGGTCA GCAGCCAAAG GGGACCACCC CTGCTCCAGC CTGCTCCACA GGTGTCCATT 
GACCTGACAG AGGAAGTGGA GCTCTTGGGA GAAGGGAGTG TGGAGAACGT CAACCCAGGA 
CCTTCAGAGG AACATACACA GGAATCTGGT GCTAACCGTA CCCTTGGAGT GTCTTCGTTG 
AATTCAGTGA ACAGCTTCAT CAGTGGGCTG CAGAGGCTTC ATGGCATGCT GCAATTCCTA 
AGACCACCCT CAGACAGTGG TGTGGGGCCA ATAAGAGCGA GGAGGAGGAG GTCTGCTTCA 
CACAGGACAC GAGTCGGAGG GTCTCAGAGG ACAGACAATG CCAGGTCAAG AGCACCATTG 
GATGCTTACT TTCAAGTGAG CAGGACCCAG CCTCACTTGC CAGCTACCTC TAATGATTCA 
GAGACCAGGA ATCCTGTCTC TGCAGACTTG CAGGTATCAA GTAGGTCCGA TTCTGACAGT 
GACAGCTCCA CAGAGAATGA AGAGGTTGTT GTCCTGGCGG AGGAACCTGG AGCTGTCGTT 
TTAGAAGAGC AAGTAGGAGG TATGTCAGCA GAGCAAGAAG TCACATGTAT CAATGGAGAA 
GAGACCCTCC CCAAACAGTC TCCCCAGAAG TCCAGCCCCC TTGTACCTTC TGTTTCTATG 
GATGAGGAAG AAGGGGACAC CTGCACAATA TGTTTGGAAC AGTGGACCAA TGTTGGGGAT 
CACCGACTCT CAGCATTACG CTGTGGACAC CTCTTTGGAT ATGGGTGCAT TTCTAAGTGG 
CTTAAAGGAC AGGCTCGAAA ATGTCCCCAG TGCAACAAGA AAGCCAAGCA AAGTGACATC 
GTTGTCCTTT ACGCCCGAAC CTTGAAAGCT TTGGACACTA GTGAACATGA GCACATGAAA 
AGTTCCTTAC TGAAGGAGCA GATGCTGCGG CAACAGGCCG AGCTAGAGTC AGCACAGTGC 
CGGCTCCAGC TGCAAGTCCT CACTGAGGAG TGCACCAGGC TTCATAGTCG TGTCCAGGAC 
TTGCAAAAAC TTATTGTGCA GCATCAGGAT CAGATTTCAC AGCAACCCAG GGGCTCCCAA 
GCACGTTTAC AAAACAGCGT GCCCTCCAGC CAGGGCCAGC ACAAGTACCA TTTCCAGAAG 
ACCTTCACGG TGTCTCAGAC AGGAAACTGC CGAATTATGA CATACTGTGA TGCTCTGGGC 
TGCCTGGTGG TGTCACAACC TTCTCCTCAG GCCTCCTTCC TTCCAGGCTT TGGCGTTAAG 
ATGTTGAGTA CTGCCAACAT GAAGAGCAGT CAGTACATTC CCATGCATAG CAAACAGATC 
CGCGGACTGG CTTTCAGCAG TCGGTCCAAA GGCTTGCTGC TCTCTGCTTC TCTGGACAGC 
ACTATTAAAC TGACCAGCCT GGAAACAAAT ACCGTGGTCC AGACTTATAA CGCTGGACGT 
CCTGTCTGGA GCTGCTGCTG GTGTCTTGAT GAAAACAATT ACATGTATGC CGGACTGGTC 
AATGGTTCAA TTCTGGTGTA TGACCTTCGA AACACGAGCA GTCATATCCA GGAGTTAGTA 
CCTCAGAAAG CCAGATGCCC ACTGGTATCC TTGTCATACA TACCCCGAGC TGCCTCAGCT 
ACATTTCCGT ATGGTGGAGT GTTGGCTGGA ACCTTGGAGA ACGCTTCCTT CTGGGAACTG 
AAAATGGGCT TTTCTCATTG GCCTCATGTG CTGCCTGTGG AGCCAGGGGG CTGCATAGAC 
TTTCAGACAG AGAGCAGTAC CCGCCACTGT CTTGTGACCT ACAGGCCTGA TAAAAATCAT 
ACCACCTTAC GAAGCGTGCT GATGGAGATG TCCTACAAGC TGGATGATGC TGGAGAGCCA 
GTTTGCTCCT GCCATCCTGT ACAAACATTC CTTGGGGGAC CCACTTGCAA ACTGCTAACC 
AAAAGTGCCA TTTTCCAAAA CCCAAAGAAT GATGGCAACA TCCTGGTGTG TACTGGGGAT 
GAAGCGTCAA ATTCTGCCCT GCTGTGGGAT GCTGGGAGTG GCTCCTTGCT ACAGGATCTG 
CAGACCCATC AGCCTGTCTT GGACATCTGC CCATTTGAGG TGAACCATAG CAGCTACTTG 
GCTACCTTAA CAGAGAAGAT GGTCCACCTC TATAGGTGGG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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RFWD3 cDNA ORF clone, Equus caballus(horse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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