NFASC cDNA ORF clone, Equus caballus(horse)

The following NFASC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFASC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb30473 XM_014739507.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OHb30474 XM_014739508.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OHb30475 XM_014739509.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OHb30476 XM_014739511.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OHb30477 XM_014739512.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OHb30478 XM_014739513.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OHb30479 XM_014739514.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OHb30480 XM_014739516.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OHb30481 XM_014739517.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OHb64746 XM_023640365.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64746 XM_023640364.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64747 XM_023640366.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64748 XM_014739501.2
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64749 XM_023640367.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64750 XM_023640368.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64751 XM_023640369.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64752 XM_023640370.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64753 XM_023640371.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64754 XM_023640372.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64755 XM_023640373.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64756 XM_023640374.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64757 XM_014739509.2
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64758 XM_014739511.2
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64759 XM_014739512.2
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64760 XM_023640375.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64761 XM_023640376.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64762 XM_023640377.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHb64763 XM_023640382.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64763 XM_023640381.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64763 XM_023640380.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64763 XM_023640379.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64763 XM_023640378.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb64764 XM_023640383.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb64765 XM_023640384.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHb20196 XM_014739503.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OHb20197 XM_014739515.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OHb30469 XM_014739501.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OHb30469 XM_014739502.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OHb30470 XM_014739504.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OHb30471 XM_014739505.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OHb30472 XM_014739506.1
Latest version!
Equus caballus neurofascin (NFASC), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb30473
    Clone ID Related Accession (Same CDS sequence) XM_014739507.1
    Accession Version XM_014739507.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TTACACCAAC
    AACCAGGCGG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGCGC CATCGCCCTC
    CTGGTGCTGA TTCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGAGGCGG CAAGTACCCA
    GTGCGAGAGA AGAAGGACGT TCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGACGGCTCC
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC CCTGGATGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ACGGCGAGGG CGGCGAGGGG
    CAGTTCAATG AGGACGGCTC CTTTATCGGC CAGTACACAG TCAAAAAGGA CAAGGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACG TCACCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594993.1
    CDS196..3738
    Translation

    Target ORF information:

    RefSeq Version XM_014739507.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739507.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TTACACCAAC
    AACCAGGCGG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGCGC CATCGCCCTC
    CTGGTGCTGA TTCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGAGGCGG CAAGTACCCA
    GTGCGAGAGA AGAAGGACGT TCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGACGGCTCC
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC CCTGGATGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ACGGCGAGGG CGGCGAGGGG
    CAGTTCAATG AGGACGGCTC CTTTATCGGC CAGTACACAG TCAAAAAGGA CAAGGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACG TCACCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30474
    Clone ID Related Accession (Same CDS sequence) XM_014739508.1
    Accession Version XM_014739508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3528bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTTACA CCAACAACCA GGCGGACATC
    GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGCGCCATCG CCCTCCTGGT GCTGATTCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGA GGCGGCAAGT ACCCAGTGCG AGAGAAGAAG
    GACGTTCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGACG GCTCCTTTGA CTACAGTGAT
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCCCTGG ATGGCACCAT CAAGCAGCAG
    GAGAGTGACG ACAGCCTGGT GGACTACGGC GAGGGCGGCG AGGGGCAGTT CAATGAGGAC
    GGCTCCTTTA TCGGCCAGTA CACAGTCAAA AAGGACAAGG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACGTCACC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594994.1
    CDS196..3723
    Translation

    Target ORF information:

    RefSeq Version XM_014739508.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTTACA CCAACAACCA GGCGGACATC
    GCCACCCAGG GCTGGTTCAT CGGGCTCATG TGCGCCATCG CCCTCCTGGT GCTGATTCTG
    CTCATCGTCT GTTTCATCAA GAGGAGTCGA GGCGGCAAGT ACCCAGTGCG AGAGAAGAAG
    GACGTTCCCC TCGGCCCTGA AGACCCCAAA GAAGAGGACG GCTCCTTTGA CTACAGTGAT
    GAGGACAACA AGCCCCTGCA GGGCAGCCAG ACATCCCTGG ATGGCACCAT CAAGCAGCAG
    GAGAGTGACG ACAGCCTGGT GGACTACGGC GAGGGCGGCG AGGGGCAGTT CAATGAGGAC
    GGCTCCTTTA TCGGCCAGTA CACAGTCAAA AAGGACAAGG AGGAGACAGA GGGCAATGAA
    AGCTCAGAGG CCACGTCACC TGTCAACGCC ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30475
    Clone ID Related Accession (Same CDS sequence) XM_014739509.1
    Accession Version XM_014739509.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAGGC GGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGC GCCATCGCCC TCCTGGTGCT GATTCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGAGGC GGCAAGTACC CAGTGCGAGA GAAGAAGGAC
    GTTCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGACGGCT CCTTTGACTA CAGTGATGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCCCTGGATG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTGGTGGA CTACGGCGAG GGCGGCGAGG GGCAGTTCAA TGAGGACGGC
    TCCTTTATCG GCCAGTACAC AGTCAAAAAG GACAAGGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CGTCACCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594995.1
    CDS196..3720
    Translation

    Target ORF information:

    RefSeq Version XM_014739509.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739509.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTAC TCCAACCGCA GCTTACACCA ACAACCAGGC GGACATCGCC
    ACCCAGGGCT GGTTCATCGG GCTCATGTGC GCCATCGCCC TCCTGGTGCT GATTCTGCTC
    ATCGTCTGTT TCATCAAGAG GAGTCGAGGC GGCAAGTACC CAGTGCGAGA GAAGAAGGAC
    GTTCCCCTCG GCCCTGAAGA CCCCAAAGAA GAGGACGGCT CCTTTGACTA CAGTGATGAG
    GACAACAAGC CCCTGCAGGG CAGCCAGACA TCCCTGGATG GCACCATCAA GCAGCAGGAG
    AGTGACGACA GCCTGGTGGA CTACGGCGAG GGCGGCGAGG GGCAGTTCAA TGAGGACGGC
    TCCTTTATCG GCCAGTACAC AGTCAAAAAG GACAAGGAGG AGACAGAGGG CAATGAAAGC
    TCAGAGGCCA CGTCACCTGT CAACGCCATC TATTCTCTGG CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30476
    Clone ID Related Accession (Same CDS sequence) XM_014739511.1
    Accession Version XM_014739511.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3510bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG GTGCTGATTC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAGAAGA AGGACGTTCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA CGGCTCCTTT GACTACAGTG ATGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCCCT GGATGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG
    GTGGACTACG GCGAGGGCGG CGAGGGGCAG TTCAATGAGG ACGGCTCCTT TATCGGCCAG
    TACACAGTCA AAAAGGACAA GGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACGTCA
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594997.1
    CDS196..3705
    Translation

    Target ORF information:

    RefSeq Version XM_014739511.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739511.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG GTGCTGATTC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAGAAGA AGGACGTTCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA CGGCTCCTTT GACTACAGTG ATGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCCCT GGATGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG
    GTGGACTACG GCGAGGGCGG CGAGGGGCAG TTCAATGAGG ACGGCTCCTT TATCGGCCAG
    TACACAGTCA AAAAGGACAA GGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACGTCA
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30477
    Clone ID Related Accession (Same CDS sequence) XM_014739512.1
    Accession Version XM_014739512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CGGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACTGC GAGCAGCCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG
    GAGTGCATCG CCTCCGGGGT CCCGACACCA GACATCGCGT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTACGTAT CACCAATGTC
    TCTGAGGAAG ACTCTGGAGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGATGAACC CAAGAACCTT
    ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAGTGGGAA CCCCAAGCCC
    ACCATCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGA
    GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACGCAAA TCAGCAGCAG GGCCGTGTAC
    CAGTGCAACA CGTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CTCGGATGCT GTCACCGCGG AACCAGCTTA TTAGGGTGAT CCTGTACAAC
    CGGACGCGGC TGGACTGCCC ATTCTTTGGG TCCCCCATTC CCACGCTCCG TTGGTTTAAG
    AATGGACAAG GAAGCAACCT GGATGGAGGC AACTACCATG TCTATGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCACAA AGAGGACCAA GGCATCTACA CCTGCGTTGC CACTAACATC
    CTGGGCAAGG CAGAAAACCA GGTCCGCCTG GAAGTCAAAG ACCCCACCAG GATCTTTCGG
    ATGCCTGAGG ACCAGGTTGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACAGTGTCC TGGCTGAAGG ATGACGAGCC GCTCTACATC
    GGGAACAGGA TGAAGAAGGA AGATGACTCC CTGACCATCT TTGGTGTGGC GGAGCGGGAC
    CAGGGCAGTT ACACCTGCGT CGCCAGCACT GAGCTGGACC AAGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCAAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594998.1
    CDS196..3669
    Translation

    Target ORF information:

    RefSeq Version XM_014739512.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CGGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACTGC GAGCAGCCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG
    GAGTGCATCG CCTCCGGGGT CCCGACACCA GACATCGCGT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTACGTAT CACCAATGTC
    TCTGAGGAAG ACTCTGGAGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGATGAACC CAAGAACCTT
    ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAGTGGGAA CCCCAAGCCC
    ACCATCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGA
    GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACGCAAA TCAGCAGCAG GGCCGTGTAC
    CAGTGCAACA CGTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CTCGGATGCT GTCACCGCGG AACCAGCTTA TTAGGGTGAT CCTGTACAAC
    CGGACGCGGC TGGACTGCCC ATTCTTTGGG TCCCCCATTC CCACGCTCCG TTGGTTTAAG
    AATGGACAAG GAAGCAACCT GGATGGAGGC AACTACCATG TCTATGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCACAA AGAGGACCAA GGCATCTACA CCTGCGTTGC CACTAACATC
    CTGGGCAAGG CAGAAAACCA GGTCCGCCTG GAAGTCAAAG ACCCCACCAG GATCTTTCGG
    ATGCCTGAGG ACCAGGTTGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACAGTGTCC TGGCTGAAGG ATGACGAGCC GCTCTACATC
    GGGAACAGGA TGAAGAAGGA AGATGACTCC CTGACCATCT TTGGTGTGGC GGAGCGGGAC
    CAGGGCAGTT ACACCTGCGT CGCCAGCACT GAGCTGGACC AAGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCAAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30478
    Clone ID Related Accession (Same CDS sequence) XM_014739513.1
    Accession Version XM_014739513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTACTCC AACCGCAGCT TACACCAACA ACCAGGCGGA CATCGCCACC
    CAGGGCTGGT TCATCGGGCT CATGTGCGCC ATCGCCCTCC TGGTGCTGAT TCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC AAGTACCCAG TGCGAGAGAA GAAGGACGTT
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GACGGCTCCT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACATCC CTGGATGGCA CCATCAAGCA GCAGGAGAGT
    GACGACAGCC TGGTGGACTA CGGCGAGGGC GGCGAGGGGC AGTTCAATGA GGACGGCTCC
    TTTATCGGCC AGTACACAGT CAAAAAGGAC AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACGT CACCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594999.1
    CDS196..3417
    Translation

    Target ORF information:

    RefSeq Version XM_014739513.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTACTCC AACCGCAGCT TACACCAACA ACCAGGCGGA CATCGCCACC
    CAGGGCTGGT TCATCGGGCT CATGTGCGCC ATCGCCCTCC TGGTGCTGAT TCTGCTCATC
    GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC AAGTACCCAG TGCGAGAGAA GAAGGACGTT
    CCCCTCGGCC CTGAAGACCC CAAAGAAGAG GACGGCTCCT TTGACTACAG TGATGAGGAC
    AACAAGCCCC TGCAGGGCAG CCAGACATCC CTGGATGGCA CCATCAAGCA GCAGGAGAGT
    GACGACAGCC TGGTGGACTA CGGCGAGGGC GGCGAGGGGC AGTTCAATGA GGACGGCTCC
    TTTATCGGCC AGTACACAGT CAAAAAGGAC AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA
    GAGGCCACGT CACCTGTCAA CGCCATCTAT TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30479
    Clone ID Related Accession (Same CDS sequence) XM_014739514.1
    Accession Version XM_014739514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3207bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTTACAC CAACAACCAG GCGGACATCG CCACCCAGGG CTGGTTCATC
    GGGCTCATGT GCGCCATCGC CCTCCTGGTG CTGATTCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGAG GCGGCAAGTA CCCAGTGCGA GAGAAGAAGG ACGTTCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGACGG CTCCTTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CATCCCTGGA TGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG
    GACTACGGCG AGGGCGGCGA GGGGCAGTTC AATGAGGACG GCTCCTTTAT CGGCCAGTAC
    ACAGTCAAAA AGGACAAGGA GGAGACAGAG GGCAATGAAA GCTCAGAGGC CACGTCACCT
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014595000.1
    CDS196..3402
    Translation

    Target ORF information:

    RefSeq Version XM_014739514.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTTACAC CAACAACCAG GCGGACATCG CCACCCAGGG CTGGTTCATC
    GGGCTCATGT GCGCCATCGC CCTCCTGGTG CTGATTCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGAG GCGGCAAGTA CCCAGTGCGA GAGAAGAAGG ACGTTCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGACGG CTCCTTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CATCCCTGGA TGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG
    GACTACGGCG AGGGCGGCGA GGGGCAGTTC AATGAGGACG GCTCCTTTAT CGGCCAGTAC
    ACAGTCAAAA AGGACAAGGA GGAGACAGAG GGCAATGAAA GCTCAGAGGC CACGTCACCT
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30480
    Clone ID Related Accession (Same CDS sequence) XM_014739516.1
    Accession Version XM_014739516.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014595002.1
    CDS196..3366
    Translation

    Target ORF information:

    RefSeq Version XM_014739516.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30481
    Clone ID Related Accession (Same CDS sequence) XM_014739517.1
    Accession Version XM_014739517.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014595003.1
    CDS196..3351
    Translation

    Target ORF information:

    RefSeq Version XM_014739517.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739517.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64746
    Clone ID Related Accession (Same CDS sequence) XM_023640365.1 , XM_023640364.1
    Accession Version XM_023640365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496133.1
    CDS51..4442
    Translation

    Target ORF information:

    RefSeq Version XM_023640365.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64746
    Clone ID Related Accession (Same CDS sequence) XM_023640365.1 , XM_023640364.1
    Accession Version XM_023640364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4392bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496132.1
    CDS51..4442
    Translation

    Target ORF information:

    RefSeq Version XM_023640364.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64747
    Clone ID Related Accession (Same CDS sequence) XM_023640366.1
    Accession Version XM_023640366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4374bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTCTGG CTGCCCTGCC CAAAGGACGG
    CCAGACAGAC CCCGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTCACC
    TGGATCCCAG GGGATGACAA CAATAGCCCC ATCACAGACT ATGTCGTCCA GTTTGAAGAG
    GACCAGTTCC AGCCAGGGGT CTGGCATGAC CATTCCAAGT TCCCAGGCAG TGTCAACTCA
    GCTGTCCTCC AGCTGTCCCC ATATGTCAAC TACCAGTTCC GGGTCATTGC CATCAACGAG
    GTCGGCAGCA GCCACCCCAG CCTCCCATCT GAGCGCTACC GAACCAGCGG AGCACCCCCT
    GAGTCTAATC CCGCTGATGT GAAGGGAGAG GGGACCAGAA AAAACAACAT GGAGATCACG
    TGGACGCCCA TGAATGCCAC CTCCGCCTTT GGCCCCAACC TGCGCTACAT CGTGAAGTGG
    CGGCGGAGAG AGACTCGAGA GAGCTGGAAC AACGTCACAG TGTGGGGCTC CCGCTACGTG
    GTGGGTCAGA CCCCTGTCTA TGTGCCCTAC GAGATCCGAG TCCAGGCCGA AAATGACTTT
    GGGAAGGGCC CTGAGCCAGA CACGGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT
    GCGCCCACCG ACGTTAAAAT CCGAGTGCTG AACAGCACGG CCATCAGCCT TCAGTGGAAC
    CGCGTCTACT CCGACACGGT CCAGGGCCTG CTCAGAGAGT ACCGAGCCTA CTACTGGAGA
    GAGAGCAGCT TGCTGAAGAG CTTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT
    GGTGACCGCC TCCGCGGCGT GGTGCCCCGC CTCTTCCCCT ACAGCAACTA CAAGCTGGAG
    ATGGTTGTGG TCAATGGGAG AGGCGATGGG CCTCGCAGTG AGACCAAGGA GTTCACAACC
    CCAGAAGGAG TACCCAGTGC CCCCAGGCGA TTCCGAGTCC GGCAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGACCA TCCGGAACAC CCCAATGGGA TCCTGACTGG ATACACCCTC
    AGATACGTGC CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGGT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCTGTCACAG AGGAGTCGCC AGCACCCCCG
    AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACTACAGT GGGTACTGTG
    AGCAGTACTG ATGCTACTGT CACTGCTGCC ACCACTGAAG CCACAACAGT CCCCATCATC
    CCAACTGTCG CACCTGCCAC CATCGCTACC ACCACCGTCG CCACAACTAC TACAACCACT
    GCCGCCACCA CCACCACCAC CACGGAGGGT CCTCCCACCA CCACCACCGG GACTAAGATA
    CACGAATCCG CCCCGGACGA GCAGTCCATA TGGAACGTCA CAGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAAGTGTC CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGAG TCTATTCCCG GGACAACGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496134.1
    CDS51..4424
    Translation

    Target ORF information:

    RefSeq Version XM_023640366.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTCTGG CTGCCCTGCC CAAAGGACGG
    CCAGACAGAC CCCGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTCACC
    TGGATCCCAG GGGATGACAA CAATAGCCCC ATCACAGACT ATGTCGTCCA GTTTGAAGAG
    GACCAGTTCC AGCCAGGGGT CTGGCATGAC CATTCCAAGT TCCCAGGCAG TGTCAACTCA
    GCTGTCCTCC AGCTGTCCCC ATATGTCAAC TACCAGTTCC GGGTCATTGC CATCAACGAG
    GTCGGCAGCA GCCACCCCAG CCTCCCATCT GAGCGCTACC GAACCAGCGG AGCACCCCCT
    GAGTCTAATC CCGCTGATGT GAAGGGAGAG GGGACCAGAA AAAACAACAT GGAGATCACG
    TGGACGCCCA TGAATGCCAC CTCCGCCTTT GGCCCCAACC TGCGCTACAT CGTGAAGTGG
    CGGCGGAGAG AGACTCGAGA GAGCTGGAAC AACGTCACAG TGTGGGGCTC CCGCTACGTG
    GTGGGTCAGA CCCCTGTCTA TGTGCCCTAC GAGATCCGAG TCCAGGCCGA AAATGACTTT
    GGGAAGGGCC CTGAGCCAGA CACGGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT
    GCGCCCACCG ACGTTAAAAT CCGAGTGCTG AACAGCACGG CCATCAGCCT TCAGTGGAAC
    CGCGTCTACT CCGACACGGT CCAGGGCCTG CTCAGAGAGT ACCGAGCCTA CTACTGGAGA
    GAGAGCAGCT TGCTGAAGAG CTTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT
    GGTGACCGCC TCCGCGGCGT GGTGCCCCGC CTCTTCCCCT ACAGCAACTA CAAGCTGGAG
    ATGGTTGTGG TCAATGGGAG AGGCGATGGG CCTCGCAGTG AGACCAAGGA GTTCACAACC
    CCAGAAGGAG TACCCAGTGC CCCCAGGCGA TTCCGAGTCC GGCAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGACCA TCCGGAACAC CCCAATGGGA TCCTGACTGG ATACACCCTC
    AGATACGTGC CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGGT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCTGTCACAG AGGAGTCGCC AGCACCCCCG
    AATGAAGCTA CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACTACAGT GGGTACTGTG
    AGCAGTACTG ATGCTACTGT CACTGCTGCC ACCACTGAAG CCACAACAGT CCCCATCATC
    CCAACTGTCG CACCTGCCAC CATCGCTACC ACCACCGTCG CCACAACTAC TACAACCACT
    GCCGCCACCA CCACCACCAC CACGGAGGGT CCTCCCACCA CCACCACCGG GACTAAGATA
    CACGAATCCG CCCCGGACGA GCAGTCCATA TGGAACGTCA CAGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAAGTGTC CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGAG TCTATTCCCG GGACAACGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64748
    Clone ID Related Accession (Same CDS sequence) XM_014739501.2
    Accession Version XM_014739501.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014739501.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGGA CGGCCAGACA GACCCCGGGA CCTGGAGCTC
    ACTGACCTGG CTGAGAGGAG TGTGAGGCTC ACCTGGATCC CAGGGGATGA CAACAATAGC
    CCCATCACAG ACTATGTCGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCAGG CAGTGTCAAC TCAGCTGTCC TCCAGCTGTC CCCATATGTC
    AACTACCAGT TCCGGGTCAT TGCCATCAAC GAGGTCGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCTGAGTCTA ATCCCGCTGA TGTGAAGGGA
    GAGGGGACCA GAAAAAACAA CATGGAGATC ACGTGGACGC CCATGAATGC CACCTCCGCC
    TTTGGCCCCA ACCTGCGCTA CATCGTGAAG TGGCGGCGGA GAGAGACTCG AGAGAGCTGG
    AACAACGTCA CAGTGTGGGG CTCCCGCTAC GTGGTGGGTC AGACCCCTGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC CGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACGGTC
    ATCGGTTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CCGACGTTAA AATCCGAGTG
    CTGAACAGCA CGGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CTGCTCAGAG AGTACCGAGC CTACTACTGG AGAGAGAGCA GCTTGCTGAA GAGCTTGTGG
    GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GCCTCCGCGG CGTGGTGCCC
    CGCCTCTTCC CCTACAGCAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGCGAT
    GGGCCTCGCA GTGAGACCAA GGAGTTCACA ACCCCAGAAG GAGTACCCAG TGCCCCCAGG
    CGATTCCGAG TCCGGCAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA CCATCCGGAA
    CACCCCAATG GGATCCTGAC TGGATACACC CTCAGATACG TGCCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTACC CTCAGTGCCA GGACGCAGGT GGGCTCTGGG
    GAAGCTGTCA CAGAGGAGTC GCCAGCACCC CCGAATGAAG CTACTCCAAC CGCAGCTCCT
    CCCACGTTGC CCCCGACTAC AGTGGGTACT GTGAGCAGTA CTGATGCTAC TGTCACTGCT
    GCCACCACTG AAGCCACAAC AGTCCCCATC ATCCCAACTG TCGCACCTGC CACCATCGCT
    ACCACCACCG TCGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACGGAG
    GGTCCTCCCA CCACCACCAC CGGGACTAAG ATACACGAAT CCGCCCCGGA CGAGCAGTCC
    ATATGGAACG TCACAGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT
    TTCGGGCCCG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAAGT
    GTCCCTGTTA AGGCCCAGGC GCAGCCTGTC CAGCTGACAG ACCTCTATCC CGGGATGACA
    TACACGTTGC GAGTCTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT
    ATGACCAGTA CAGCTTACAC CAACAACCAG GCGGACATCG CCACCCAGGG CTGGTTCATC
    GGGCTCATGT GCGCCATCGC CCTCCTGGTG CTGATTCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGAG GCGGCAAGTA CCCAGTGCGA GAGAAGAAGG ACGTTCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGACGG CTCCTTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CATCCCTGGA TGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG
    GACTACGGCG AGGGCGGCGA GGGGCAGTTC AATGAGGACG GCTCCTTTAT CGGCCAGTAC
    ACAGTCAAAA AGGACAAGGA GGAGACAGAG GGCAATGAAA GCTCAGAGGC CACGTCACCT
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594987.2
    CDS51..4397
    Translation

    Target ORF information:

    RefSeq Version XM_014739501.2
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739501.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGGA CGGCCAGACA GACCCCGGGA CCTGGAGCTC
    ACTGACCTGG CTGAGAGGAG TGTGAGGCTC ACCTGGATCC CAGGGGATGA CAACAATAGC
    CCCATCACAG ACTATGTCGT CCAGTTTGAA GAGGACCAGT TCCAGCCAGG GGTCTGGCAT
    GACCATTCCA AGTTCCCAGG CAGTGTCAAC TCAGCTGTCC TCCAGCTGTC CCCATATGTC
    AACTACCAGT TCCGGGTCAT TGCCATCAAC GAGGTCGGCA GCAGCCACCC CAGCCTCCCA
    TCTGAGCGCT ACCGAACCAG CGGAGCACCC CCTGAGTCTA ATCCCGCTGA TGTGAAGGGA
    GAGGGGACCA GAAAAAACAA CATGGAGATC ACGTGGACGC CCATGAATGC CACCTCCGCC
    TTTGGCCCCA ACCTGCGCTA CATCGTGAAG TGGCGGCGGA GAGAGACTCG AGAGAGCTGG
    AACAACGTCA CAGTGTGGGG CTCCCGCTAC GTGGTGGGTC AGACCCCTGT CTATGTGCCC
    TACGAGATCC GAGTCCAGGC CGAAAATGAC TTTGGGAAGG GCCCTGAGCC AGACACGGTC
    ATCGGTTACT CCGGAGAAGA TTATCCCAGG GCTGCGCCCA CCGACGTTAA AATCCGAGTG
    CTGAACAGCA CGGCCATCAG CCTTCAGTGG AACCGCGTCT ACTCCGACAC GGTCCAGGGC
    CTGCTCAGAG AGTACCGAGC CTACTACTGG AGAGAGAGCA GCTTGCTGAA GAGCTTGTGG
    GTGTCTCAGA AGAGACAGCA GGCCAGCTTC CCTGGTGACC GCCTCCGCGG CGTGGTGCCC
    CGCCTCTTCC CCTACAGCAA CTACAAGCTG GAGATGGTTG TGGTCAATGG GAGAGGCGAT
    GGGCCTCGCA GTGAGACCAA GGAGTTCACA ACCCCAGAAG GAGTACCCAG TGCCCCCAGG
    CGATTCCGAG TCCGGCAGCC CAACCTGGAG ACCATCAACC TGGAATGGGA CCATCCGGAA
    CACCCCAATG GGATCCTGAC TGGATACACC CTCAGATACG TGCCCTTTAA TGGAACCAAA
    GTAGGAAAGC AGATAGTGGA AAACTTCTCT CCCAATCAGA CCAAGTTCAC GGTGCAAAGA
    GCAGACCCCG TGTCGCGCTA CCGCTTTACC CTCAGTGCCA GGACGCAGGT GGGCTCTGGG
    GAAGCTGTCA CAGAGGAGTC GCCAGCACCC CCGAATGAAG CTACTCCAAC CGCAGCTCCT
    CCCACGTTGC CCCCGACTAC AGTGGGTACT GTGAGCAGTA CTGATGCTAC TGTCACTGCT
    GCCACCACTG AAGCCACAAC AGTCCCCATC ATCCCAACTG TCGCACCTGC CACCATCGCT
    ACCACCACCG TCGCCACAAC TACTACAACC ACTGCCGCCA CCACCACCAC CACCACGGAG
    GGTCCTCCCA CCACCACCAC CGGGACTAAG ATACACGAAT CCGCCCCGGA CGAGCAGTCC
    ATATGGAACG TCACAGTGCT CCCCAACAGT AAATGGGCCA ACATCACCTG GAAGCACAAT
    TTCGGGCCCG GAACTGACTT TGTGGTTGAG TACATCGACA GCAACCATAC GAAAAAAAGT
    GTCCCTGTTA AGGCCCAGGC GCAGCCTGTC CAGCTGACAG ACCTCTATCC CGGGATGACA
    TACACGTTGC GAGTCTATTC CCGGGACAAC GAGGGCATCA GCAGTACCGT CATCACCTTT
    ATGACCAGTA CAGCTTACAC CAACAACCAG GCGGACATCG CCACCCAGGG CTGGTTCATC
    GGGCTCATGT GCGCCATCGC CCTCCTGGTG CTGATTCTGC TCATCGTCTG TTTCATCAAG
    AGGAGTCGAG GCGGCAAGTA CCCAGTGCGA GAGAAGAAGG ACGTTCCCCT CGGCCCTGAA
    GACCCCAAAG AAGAGGACGG CTCCTTTGAC TACAGTGATG AGGACAACAA GCCCCTGCAG
    GGCAGCCAGA CATCCCTGGA TGGCACCATC AAGCAGCAGG AGAGTGACGA CAGCCTGGTG
    GACTACGGCG AGGGCGGCGA GGGGCAGTTC AATGAGGACG GCTCCTTTAT CGGCCAGTAC
    ACAGTCAAAA AGGACAAGGA GGAGACAGAG GGCAATGAAA GCTCAGAGGC CACGTCACCT
    GTCAACGCCA TCTATTCTCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64749
    Clone ID Related Accession (Same CDS sequence) XM_023640367.1
    Accession Version XM_023640367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4341bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CACCCGAGGA
    GTTGCGGAAA GGACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG CCAGATGGTG
    CTGCGTGGCA TGGACCTCCT GCTGGAGTGC ATCGCCTCCG GGGTCCCGAC ACCAGACATC
    GCGTGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTTGA GAACTTTAAC
    AAGGCTCTAC GTATCACCAA TGTCTCTGAG GAAGACTCTG GAGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTTATCCTG GCTCCTGGCG AGGACGGGAG ACTGGTGTGT
    CGAGCCAGTG GGAACCCCAA GCCCACCATC CAGTGGATGG TGAATGGGGA ACCTTTGCAA
    TCGGCACCAC CCAACCCAAA CCGAGAGGTG GCCGGGGACA CCATCATCTT CCGGGACACG
    CAGATCAGCA GCAGGGCCGT GTACCAGTGC AACACGTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCACCTCGGA TGCTGTCACC GCGGAACCAG
    CTTATTAGGG TGATCCTGTA CAACCGGACG CGGCTGGACT GCCCATTCTT TGGGTCCCCC
    ATTCCCACGC TCCGTTGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG AGGCAACTAC
    CATGTCTATG AGAATGGCAG TCTGGAAATC AAGATGATCC ACAAAGAGGA CCAAGGCATC
    TACACCTGCG TTGCCACTAA CATCCTGGGC AAGGCAGAAA ACCAGGTCCG CCTGGAAGTC
    AAAGACCCCA CCAGGATCTT TCGGATGCCT GAGGACCAGG TTGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGGGT GAAGCACGAC CCCTCCCTGA AGCTCACAGT GTCCTGGCTG
    AAGGATGACG AGCCGCTCTA CATCGGGAAC AGGATGAAGA AGGAAGATGA CTCCCTGACC
    ATCTTTGGTG TGGCGGAGCG GGACCAGGGC AGTTACACCT GCGTCGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAGGC CTACCTCACC GTGCTAGCTG ATCAGGCCAC TCCAACTAAC
    CGTCTGGCTG CCCTGCCCAA AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496135.1
    CDS51..4391
    Translation

    Target ORF information:

    RefSeq Version XM_023640367.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CACCCGAGGA
    GTTGCGGAAA GGACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG CCAGATGGTG
    CTGCGTGGCA TGGACCTCCT GCTGGAGTGC ATCGCCTCCG GGGTCCCGAC ACCAGACATC
    GCGTGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTTGA GAACTTTAAC
    AAGGCTCTAC GTATCACCAA TGTCTCTGAG GAAGACTCTG GAGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTTATCCTG GCTCCTGGCG AGGACGGGAG ACTGGTGTGT
    CGAGCCAGTG GGAACCCCAA GCCCACCATC CAGTGGATGG TGAATGGGGA ACCTTTGCAA
    TCGGCACCAC CCAACCCAAA CCGAGAGGTG GCCGGGGACA CCATCATCTT CCGGGACACG
    CAGATCAGCA GCAGGGCCGT GTACCAGTGC AACACGTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCACCTCGGA TGCTGTCACC GCGGAACCAG
    CTTATTAGGG TGATCCTGTA CAACCGGACG CGGCTGGACT GCCCATTCTT TGGGTCCCCC
    ATTCCCACGC TCCGTTGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG AGGCAACTAC
    CATGTCTATG AGAATGGCAG TCTGGAAATC AAGATGATCC ACAAAGAGGA CCAAGGCATC
    TACACCTGCG TTGCCACTAA CATCCTGGGC AAGGCAGAAA ACCAGGTCCG CCTGGAAGTC
    AAAGACCCCA CCAGGATCTT TCGGATGCCT GAGGACCAGG TTGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGGGT GAAGCACGAC CCCTCCCTGA AGCTCACAGT GTCCTGGCTG
    AAGGATGACG AGCCGCTCTA CATCGGGAAC AGGATGAAGA AGGAAGATGA CTCCCTGACC
    ATCTTTGGTG TGGCGGAGCG GGACCAGGGC AGTTACACCT GCGTCGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAGGC CTACCTCACC GTGCTAGCTG ATCAGGCCAC TCCAACTAAC
    CGTCTGGCTG CCCTGCCCAA AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCGAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64750
    Clone ID Related Accession (Same CDS sequence) XM_023640368.1
    Accession Version XM_023640368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CCCCGGACGA GCAGTCCATA
    TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC
    GGGCCCGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAAGTGTC
    CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG CTGACAGACC TCTATCCCGG GATGACATAC
    ACGTTGCGAG TCTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG
    ACCAGTACAG CTTACACCAA CAACCAGGCG GACATCGCCA CCCAGGGCTG GTTCATCGGG
    CTCATGTGCG CCATCGCCCT CCTGGTGCTG ATTCTGCTCA TCGTCTGTTT CATCAAGAGG
    AGTCGAGGCG GCAAGTACCC AGTGCGAGAG AAGAAGGACG TTCCCCTCGG CCCTGAAGAC
    CCCAAAGAAG AGGACGGCTC CTTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC
    AGCCAGACAT CCCTGGATGG CACCATCAAG CAGCAGGAGA GTGACGACAG CCTGGTGGAC
    TACGGCGAGG GCGGCGAGGG GCAGTTCAAT GAGGACGGCT CCTTTATCGG CCAGTACACA
    GTCAAAAAGG ACAAGGAGGA GACAGAGGGC AATGAAAGCT CAGAGGCCAC GTCACCTGTC
    AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496136.1
    CDS50..4213
    Translation

    Target ORF information:

    RefSeq Version XM_023640368.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CCCCGGACGA GCAGTCCATA
    TGGAACGTCA CAGTGCTCCC CAACAGTAAA TGGGCCAACA TCACCTGGAA GCACAATTTC
    GGGCCCGGAA CTGACTTTGT GGTTGAGTAC ATCGACAGCA ACCATACGAA AAAAAGTGTC
    CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG CTGACAGACC TCTATCCCGG GATGACATAC
    ACGTTGCGAG TCTATTCCCG GGACAACGAG GGCATCAGCA GTACCGTCAT CACCTTTATG
    ACCAGTACAG CTTACACCAA CAACCAGGCG GACATCGCCA CCCAGGGCTG GTTCATCGGG
    CTCATGTGCG CCATCGCCCT CCTGGTGCTG ATTCTGCTCA TCGTCTGTTT CATCAAGAGG
    AGTCGAGGCG GCAAGTACCC AGTGCGAGAG AAGAAGGACG TTCCCCTCGG CCCTGAAGAC
    CCCAAAGAAG AGGACGGCTC CTTTGACTAC AGTGATGAGG ACAACAAGCC CCTGCAGGGC
    AGCCAGACAT CCCTGGATGG CACCATCAAG CAGCAGGAGA GTGACGACAG CCTGGTGGAC
    TACGGCGAGG GCGGCGAGGG GCAGTTCAAT GAGGACGGCT CCTTTATCGG CCAGTACACA
    GTCAAAAAGG ACAAGGAGGA GACAGAGGGC AATGAAAGCT CAGAGGCCAC GTCACCTGTC
    AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64751
    Clone ID Related Accession (Same CDS sequence) XM_023640369.1
    Accession Version XM_023640369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4071bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTTAC CCAGTGCCCC CAGGCGATTC CGAGTCCGGC AGCCCAACCT GGAGACCATC
    AACCTGGAAT GGGACCATCC GGAACACCCC AATGGGATCC TGACTGGATA CACCCTCAGA
    TACGTGCCCT TTAATGGAAC CAAAGTAGGA AAGCAGATAG TGGAAAACTT CTCTCCCAAT
    CAGACCAAGT TCACGGTGCA AAGAGCAGAC CCCGTGTCGC GCTACCGCTT TACCCTCAGT
    GCCAGGACGC AGGTGGGCTC TGGGGAAGCT GTCACAGAGG AGTCGCCAGC ACCCCCGAAT
    GAAGCTACTC CAACCGCAGC TCCTCCCACG TTGCCCCCGA CTACAGTGGG TACTGTGAGC
    AGTACTGATG CTACTGTCAC TGCTGCCACC ACTGAAGCCA CAACAGTCCC CATCATCCCA
    ACTGTCGCAC CTGCCACCAT CGCTACCACC ACCGTCGCCA CAACTACTAC AACCACTGCC
    GCCACCACCA CCACCACCAC GGAGGGTCCT CCCACCACCA CCACCGGGAC TAAGATACAC
    GAATCCGCCC CGGACGAGCA GTCCATATGG AACGTCACAG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAAGCA CAATTTCGGG CCCGGAACTG ACTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AAGTGTCCCT GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGAGTCT ATTCCCGGGA CAACGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496137.1
    CDS50..4120
    Translation

    Target ORF information:

    RefSeq Version XM_023640369.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTTAC CCAGTGCCCC CAGGCGATTC CGAGTCCGGC AGCCCAACCT GGAGACCATC
    AACCTGGAAT GGGACCATCC GGAACACCCC AATGGGATCC TGACTGGATA CACCCTCAGA
    TACGTGCCCT TTAATGGAAC CAAAGTAGGA AAGCAGATAG TGGAAAACTT CTCTCCCAAT
    CAGACCAAGT TCACGGTGCA AAGAGCAGAC CCCGTGTCGC GCTACCGCTT TACCCTCAGT
    GCCAGGACGC AGGTGGGCTC TGGGGAAGCT GTCACAGAGG AGTCGCCAGC ACCCCCGAAT
    GAAGCTACTC CAACCGCAGC TCCTCCCACG TTGCCCCCGA CTACAGTGGG TACTGTGAGC
    AGTACTGATG CTACTGTCAC TGCTGCCACC ACTGAAGCCA CAACAGTCCC CATCATCCCA
    ACTGTCGCAC CTGCCACCAT CGCTACCACC ACCGTCGCCA CAACTACTAC AACCACTGCC
    GCCACCACCA CCACCACCAC GGAGGGTCCT CCCACCACCA CCACCGGGAC TAAGATACAC
    GAATCCGCCC CGGACGAGCA GTCCATATGG AACGTCACAG TGCTCCCCAA CAGTAAATGG
    GCCAACATCA CCTGGAAGCA CAATTTCGGG CCCGGAACTG ACTTTGTGGT TGAGTACATC
    GACAGCAACC ATACGAAAAA AAGTGTCCCT GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG
    ACAGACCTCT ATCCCGGGAT GACATACACG TTGCGAGTCT ATTCCCGGGA CAACGAGGGC
    ATCAGCAGTA CCGTCATCAC CTTTATGACC AGTACAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64752
    Clone ID Related Accession (Same CDS sequence) XM_023640370.1
    Accession Version XM_023640370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4020bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CACCCGAGGA
    GTTGCGGAAA GGACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG CCAGATGGTG
    CTGCGTGGCA TGGACCTCCT GCTGGAGTGC ATCGCCTCCG GGGTCCCGAC ACCAGACATC
    GCGTGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTTGA GAACTTTAAC
    AAGGCTCTAC GTATCACCAA TGTCTCTGAG GAAGACTCTG GAGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTTATCCTG GCTCCTGGCG AGGACGGGAG ACTGGTGTGT
    CGAGCCAGTG GGAACCCCAA GCCCACCATC CAGTGGATGG TGAATGGGGA ACCTTTGCAA
    TCGGCACCAC CCAACCCAAA CCGAGAGGTG GCCGGGGACA CCATCATCTT CCGGGACACG
    CAGATCAGCA GCAGGGCCGT GTACCAGTGC AACACGTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCACCTCGGA TGCTGTCACC GCGGAACCAG
    CTTATTAGGG TGATCCTGTA CAACCGGACG CGGCTGGACT GCCCATTCTT TGGGTCCCCC
    ATTCCCACGC TCCGTTGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG AGGCAACTAC
    CATGTCTATG AGAATGGCAG TCTGGAAATC AAGATGATCC ACAAAGAGGA CCAAGGCATC
    TACACCTGCG TTGCCACTAA CATCCTGGGC AAGGCAGAAA ACCAGGTCCG CCTGGAAGTC
    AAAGACCCCA CCAGGATCTT TCGGATGCCT GAGGACCAGG TTGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGGGT GAAGCACGAC CCCTCCCTGA AGCTCACAGT GTCCTGGCTG
    AAGGATGACG AGCCGCTCTA CATCGGGAAC AGGATGAAGA AGGAAGATGA CTCCCTGACC
    ATCTTTGGTG TGGCGGAGCG GGACCAGGGC AGTTACACCT GCGTCGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAGGC CTACCTCACC GTGCTAGCTG ATCAGGCCAC TCCAACTAAC
    CGTCTGGCTG CCCTGCCCAA AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCTACTCC AACCGCAGCT CCTCCCACGT TGCCCCCGAC TACAGTGGGT
    ACTGTGAGCA GTACTGATGC TACTGTCACT GCTGCCACCA CTGAAGCCAC AACAGTCCCC
    ATCATCCCAA CTGTCGCACC TGCCACCATC GCTACCACCA CCGTCGCCAC AACTACTACA
    ACCACTGCCG CCACCACCAC CACCACCACG GAGGGTCCTC CCACCACCAC CACCGGGACT
    AAGATACACG AATCCGCCCC GGACGAGCAG TCCATATGGA ACGTCACAGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACATCG ACAGCAACCA TACGAAAAAA AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT
    GTCCAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGAGTCTA TTCCCGGGAC
    AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496138.1
    CDS49..4068
    Translation

    Target ORF information:

    RefSeq Version XM_023640370.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CACCCGAGGA
    GTTGCGGAAA GGACACCCAG CTTCATGTAT CCCCAGGGCA CTGCGAGCAG CCAGATGGTG
    CTGCGTGGCA TGGACCTCCT GCTGGAGTGC ATCGCCTCCG GGGTCCCGAC ACCAGACATC
    GCGTGGTACA AGAAAGGTGG GGACCTCCCA TCTGACAAGG CCAAGTTTGA GAACTTTAAC
    AAGGCTCTAC GTATCACCAA TGTCTCTGAG GAAGACTCTG GAGAGTATTT CTGCCTGGCC
    TCCAACAAGA TGGGCAGCAT CCGGCACACG ATCTCGGTGA GAGTAAAGGC TGCTCCCTAC
    TGGCTGGATG AACCCAAGAA CCTTATCCTG GCTCCTGGCG AGGACGGGAG ACTGGTGTGT
    CGAGCCAGTG GGAACCCCAA GCCCACCATC CAGTGGATGG TGAATGGGGA ACCTTTGCAA
    TCGGCACCAC CCAACCCAAA CCGAGAGGTG GCCGGGGACA CCATCATCTT CCGGGACACG
    CAGATCAGCA GCAGGGCCGT GTACCAGTGC AACACGTCCA ACGAGCACGG CTACCTGCTG
    GCCAACGCCT TCGTCAGCGT GCTGGACGTG CCACCTCGGA TGCTGTCACC GCGGAACCAG
    CTTATTAGGG TGATCCTGTA CAACCGGACG CGGCTGGACT GCCCATTCTT TGGGTCCCCC
    ATTCCCACGC TCCGTTGGTT TAAGAATGGA CAAGGAAGCA ACCTGGATGG AGGCAACTAC
    CATGTCTATG AGAATGGCAG TCTGGAAATC AAGATGATCC ACAAAGAGGA CCAAGGCATC
    TACACCTGCG TTGCCACTAA CATCCTGGGC AAGGCAGAAA ACCAGGTCCG CCTGGAAGTC
    AAAGACCCCA CCAGGATCTT TCGGATGCCT GAGGACCAGG TTGCCAAGAG GGGCACCACG
    GTGCAGCTGG AGTGCCGGGT GAAGCACGAC CCCTCCCTGA AGCTCACAGT GTCCTGGCTG
    AAGGATGACG AGCCGCTCTA CATCGGGAAC AGGATGAAGA AGGAAGATGA CTCCCTGACC
    ATCTTTGGTG TGGCGGAGCG GGACCAGGGC AGTTACACCT GCGTCGCCAG CACTGAGCTG
    GACCAAGACC TGGCCAAGGC CTACCTCACC GTGCTAGCTG ATCAGGCCAC TCCAACTAAC
    CGTCTGGCTG CCCTGCCCAA AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCGAATG AAGCTACTCC AACCGCAGCT CCTCCCACGT TGCCCCCGAC TACAGTGGGT
    ACTGTGAGCA GTACTGATGC TACTGTCACT GCTGCCACCA CTGAAGCCAC AACAGTCCCC
    ATCATCCCAA CTGTCGCACC TGCCACCATC GCTACCACCA CCGTCGCCAC AACTACTACA
    ACCACTGCCG CCACCACCAC CACCACCACG GAGGGTCCTC CCACCACCAC CACCGGGACT
    AAGATACACG AATCCGCCCC GGACGAGCAG TCCATATGGA ACGTCACAGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACATCG ACAGCAACCA TACGAAAAAA AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT
    GTCCAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGAGTCTA TTCCCGGGAC
    AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64753
    Clone ID Related Accession (Same CDS sequence) XM_023640371.1
    Accession Version XM_023640371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496139.1
    CDS48..3941
    Translation

    Target ORF information:

    RefSeq Version XM_023640371.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64754
    Clone ID Related Accession (Same CDS sequence) XM_023640372.1
    Accession Version XM_023640372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3879bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTTAC ACCAACAACC AGGCGGACAT CGCCACCCAG
    GGCTGGTTCA TCGGGCTCAT GTGCGCCATC GCCCTCCTGG TGCTGATTCT GCTCATCGTC
    TGTTTCATCA AGAGGAGTCG AGGCGGCAAG TACCCAGTGC GAGAGAAGAA GGACGTTCCC
    CTCGGCCCTG AAGACCCCAA AGAAGAGGAC GGCTCCTTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGCAGCCA GACATCCCTG GATGGCACCA TCAAGCAGCA GGAGAGTGAC
    GACAGCCTGG TGGACTACGG CGAGGGCGGC GAGGGGCAGT TCAATGAGGA CGGCTCCTTT
    ATCGGCCAGT ACACAGTCAA AAAGGACAAG GAGGAGACAG AGGGCAATGA AAGCTCAGAG
    GCCACGTCAC CTGTCAACGC CATCTATTCT CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496140.1
    CDS48..3926
    Translation

    Target ORF information:

    RefSeq Version XM_023640372.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTTAC ACCAACAACC AGGCGGACAT CGCCACCCAG
    GGCTGGTTCA TCGGGCTCAT GTGCGCCATC GCCCTCCTGG TGCTGATTCT GCTCATCGTC
    TGTTTCATCA AGAGGAGTCG AGGCGGCAAG TACCCAGTGC GAGAGAAGAA GGACGTTCCC
    CTCGGCCCTG AAGACCCCAA AGAAGAGGAC GGCTCCTTTG ACTACAGTGA TGAGGACAAC
    AAGCCCCTGC AGGGCAGCCA GACATCCCTG GATGGCACCA TCAAGCAGCA GGAGAGTGAC
    GACAGCCTGG TGGACTACGG CGAGGGCGGC GAGGGGCAGT TCAATGAGGA CGGCTCCTTT
    ATCGGCCAGT ACACAGTCAA AAAGGACAAG GAGGAGACAG AGGGCAATGA AAGCTCAGAG
    GCCACGTCAC CTGTCAACGC CATCTATTCT CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64755
    Clone ID Related Accession (Same CDS sequence) XM_023640373.1
    Accession Version XM_023640373.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTCTGG CTGCCCTGCC CAAAGGACGG
    CCAGACAGAC CCCGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTCACC
    TGGATCCCAG GGGATGACAA CAATAGCCCC ATCACAGACT ATGTCGTCCA GTTTGAAGAG
    GACCAGTTCC AGCCAGGGGT CTGGCATGAC CATTCCAAGT TCCCAGGCAG TGTCAACTCA
    GCTGTCCTCC AGCTGTCCCC ATATGTCAAC TACCAGTTCC GGGTCATTGC CATCAACGAG
    GTCGGCAGCA GCCACCCCAG CCTCCCATCT GAGCGCTACC GAACCAGCGG AGCACCCCCT
    GAGTCTAATC CCGCTGATGT GAAGGGAGAG GGGACCAGAA AAAACAACAT GGAGATCACG
    TGGACGCCCA TGAATGCCAC CTCCGCCTTT GGCCCCAACC TGCGCTACAT CGTGAAGTGG
    CGGCGGAGAG AGACTCGAGA GAGCTGGAAC AACGTCACAG TGTGGGGCTC CCGCTACGTG
    GTGGGTCAGA CCCCTGTCTA TGTGCCCTAC GAGATCCGAG TCCAGGCCGA AAATGACTTT
    GGGAAGGGCC CTGAGCCAGA CACGGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT
    GCGCCCACCG ACGTTAAAAT CCGAGTGCTG AACAGCACGG CCATCAGCCT TCAGTGGAAC
    CGCGTCTACT CCGACACGGT CCAGGGCCTG CTCAGAGAGT ACCGAGCCTA CTACTGGAGA
    GAGAGCAGCT TGCTGAAGAG CTTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT
    GGTGACCGCC TCCGCGGCGT GGTGCCCCGC CTCTTCCCCT ACAGCAACTA CAAGCTGGAG
    ATGGTTGTGG TCAATGGGAG AGGCGATGGG CCTCGCAGTG AGACCAAGGA GTTCACAACC
    CCAGAAGGAG TACCCAGTGC CCCCAGGCGA TTCCGAGTCC GGCAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGACCA TCCGGAACAC CCCAATGGGA TCCTGACTGG ATACACCCTC
    AGATACGTGC CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGGT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCTGTCACAG AGGAGTCGCC AGCACCCCCG
    AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496141.1
    CDS48..3923
    Translation

    Target ORF information:

    RefSeq Version XM_023640373.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640373.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG CTGATCAGGC CACTCCAACT AACCGTCTGG CTGCCCTGCC CAAAGGACGG
    CCAGACAGAC CCCGGGACCT GGAGCTCACT GACCTGGCTG AGAGGAGTGT GAGGCTCACC
    TGGATCCCAG GGGATGACAA CAATAGCCCC ATCACAGACT ATGTCGTCCA GTTTGAAGAG
    GACCAGTTCC AGCCAGGGGT CTGGCATGAC CATTCCAAGT TCCCAGGCAG TGTCAACTCA
    GCTGTCCTCC AGCTGTCCCC ATATGTCAAC TACCAGTTCC GGGTCATTGC CATCAACGAG
    GTCGGCAGCA GCCACCCCAG CCTCCCATCT GAGCGCTACC GAACCAGCGG AGCACCCCCT
    GAGTCTAATC CCGCTGATGT GAAGGGAGAG GGGACCAGAA AAAACAACAT GGAGATCACG
    TGGACGCCCA TGAATGCCAC CTCCGCCTTT GGCCCCAACC TGCGCTACAT CGTGAAGTGG
    CGGCGGAGAG AGACTCGAGA GAGCTGGAAC AACGTCACAG TGTGGGGCTC CCGCTACGTG
    GTGGGTCAGA CCCCTGTCTA TGTGCCCTAC GAGATCCGAG TCCAGGCCGA AAATGACTTT
    GGGAAGGGCC CTGAGCCAGA CACGGTCATC GGTTACTCCG GAGAAGATTA TCCCAGGGCT
    GCGCCCACCG ACGTTAAAAT CCGAGTGCTG AACAGCACGG CCATCAGCCT TCAGTGGAAC
    CGCGTCTACT CCGACACGGT CCAGGGCCTG CTCAGAGAGT ACCGAGCCTA CTACTGGAGA
    GAGAGCAGCT TGCTGAAGAG CTTGTGGGTG TCTCAGAAGA GACAGCAGGC CAGCTTCCCT
    GGTGACCGCC TCCGCGGCGT GGTGCCCCGC CTCTTCCCCT ACAGCAACTA CAAGCTGGAG
    ATGGTTGTGG TCAATGGGAG AGGCGATGGG CCTCGCAGTG AGACCAAGGA GTTCACAACC
    CCAGAAGGAG TACCCAGTGC CCCCAGGCGA TTCCGAGTCC GGCAGCCCAA CCTGGAGACC
    ATCAACCTGG AATGGGACCA TCCGGAACAC CCCAATGGGA TCCTGACTGG ATACACCCTC
    AGATACGTGC CCTTTAATGG AACCAAAGTA GGAAAGCAGA TAGTGGAAAA CTTCTCTCCC
    AATCAGACCA AGTTCACGGT GCAAAGAGCA GACCCCGTGT CGCGCTACCG CTTTACCCTC
    AGTGCCAGGA CGCAGGTGGG CTCTGGGGAA GCTGTCACAG AGGAGTCGCC AGCACCCCCG
    AATGAAGCTA CTCCAACCGC AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64756
    Clone ID Related Accession (Same CDS sequence) XM_023640374.1
    Accession Version XM_023640374.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3846bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG CAGCCTGTCC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496142.1
    CDS50..3895
    Translation

    Target ORF information:

    RefSeq Version XM_023640374.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640374.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCGAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG CAGCCTGTCC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64757
    Clone ID Related Accession (Same CDS sequence) XM_014739509.2
    Accession Version XM_014739509.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014739509.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG GACGGCCAGA CAGACCCCGG GACCTGGAGC TCACTGACCT GGCTGAGAGG
    AGTGTGAGGC TCACCTGGAT CCCAGGGGAT GACAACAATA GCCCCATCAC AGACTATGTC
    GTCCAGTTTG AAGAGGACCA GTTCCAGCCA GGGGTCTGGC ATGACCATTC CAAGTTCCCA
    GGCAGTGTCA ACTCAGCTGT CCTCCAGCTG TCCCCATATG TCAACTACCA GTTCCGGGTC
    ATTGCCATCA ACGAGGTCGG CAGCAGCCAC CCCAGCCTCC CATCTGAGCG CTACCGAACC
    AGCGGAGCAC CCCCTGAGTC TAATCCCGCT GATGTGAAGG GAGAGGGGAC CAGAAAAAAC
    AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCCG CCTTTGGCCC CAACCTGCGC
    TACATCGTGA AGTGGCGGCG GAGAGAGACT CGAGAGAGCT GGAACAACGT CACAGTGTGG
    GGCTCCCGCT ACGTGGTGGG TCAGACCCCT GTCTATGTGC CCTACGAGAT CCGAGTCCAG
    GCCGAAAATG ACTTTGGGAA GGGCCCTGAG CCAGACACGG TCATCGGTTA CTCCGGAGAA
    GATTATCCCA GGGCTGCGCC CACCGACGTT AAAATCCGAG TGCTGAACAG CACGGCCATC
    AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCTGCTCAG AGAGTACCGA
    GCCTACTACT GGAGAGAGAG CAGCTTGCTG AAGAGCTTGT GGGTGTCTCA GAAGAGACAG
    CAGGCCAGCT TCCCTGGTGA CCGCCTCCGC GGCGTGGTGC CCCGCCTCTT CCCCTACAGC
    AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGCG ATGGGCCTCG CAGTGAGACC
    AAGGAGTTCA CAACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594995.2
    CDS47..3877
    Translation

    Target ORF information:

    RefSeq Version XM_014739509.2
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739509.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG GACGGCCAGA CAGACCCCGG GACCTGGAGC TCACTGACCT GGCTGAGAGG
    AGTGTGAGGC TCACCTGGAT CCCAGGGGAT GACAACAATA GCCCCATCAC AGACTATGTC
    GTCCAGTTTG AAGAGGACCA GTTCCAGCCA GGGGTCTGGC ATGACCATTC CAAGTTCCCA
    GGCAGTGTCA ACTCAGCTGT CCTCCAGCTG TCCCCATATG TCAACTACCA GTTCCGGGTC
    ATTGCCATCA ACGAGGTCGG CAGCAGCCAC CCCAGCCTCC CATCTGAGCG CTACCGAACC
    AGCGGAGCAC CCCCTGAGTC TAATCCCGCT GATGTGAAGG GAGAGGGGAC CAGAAAAAAC
    AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCCG CCTTTGGCCC CAACCTGCGC
    TACATCGTGA AGTGGCGGCG GAGAGAGACT CGAGAGAGCT GGAACAACGT CACAGTGTGG
    GGCTCCCGCT ACGTGGTGGG TCAGACCCCT GTCTATGTGC CCTACGAGAT CCGAGTCCAG
    GCCGAAAATG ACTTTGGGAA GGGCCCTGAG CCAGACACGG TCATCGGTTA CTCCGGAGAA
    GATTATCCCA GGGCTGCGCC CACCGACGTT AAAATCCGAG TGCTGAACAG CACGGCCATC
    AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCTGCTCAG AGAGTACCGA
    GCCTACTACT GGAGAGAGAG CAGCTTGCTG AAGAGCTTGT GGGTGTCTCA GAAGAGACAG
    CAGGCCAGCT TCCCTGGTGA CCGCCTCCGC GGCGTGGTGC CCCGCCTCTT CCCCTACAGC
    AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGCG ATGGGCCTCG CAGTGAGACC
    AAGGAGTTCA CAACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCTACTCCA ACCGCAGCTT ACACCAACAA CCAGGCGGAC
    ATCGCCACCC AGGGCTGGTT CATCGGGCTC ATGTGCGCCA TCGCCCTCCT GGTGCTGATT
    CTGCTCATCG TCTGTTTCAT CAAGAGGAGT CGAGGCGGCA AGTACCCAGT GCGAGAGAAG
    AAGGACGTTC CCCTCGGCCC TGAAGACCCC AAAGAAGAGG ACGGCTCCTT TGACTACAGT
    GATGAGGACA ACAAGCCCCT GCAGGGCAGC CAGACATCCC TGGATGGCAC CATCAAGCAG
    CAGGAGAGTG ACGACAGCCT GGTGGACTAC GGCGAGGGCG GCGAGGGGCA GTTCAATGAG
    GACGGCTCCT TTATCGGCCA GTACACAGTC AAAAAGGACA AGGAGGAGAC AGAGGGCAAT
    GAAAGCTCAG AGGCCACGTC ACCTGTCAAC GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64758
    Clone ID Related Accession (Same CDS sequence) XM_014739511.2
    Accession Version XM_014739511.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3816bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014739511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG GACGGCCAGA CAGACCCCGG GACCTGGAGC TCACTGACCT GGCTGAGAGG
    AGTGTGAGGC TCACCTGGAT CCCAGGGGAT GACAACAATA GCCCCATCAC AGACTATGTC
    GTCCAGTTTG AAGAGGACCA GTTCCAGCCA GGGGTCTGGC ATGACCATTC CAAGTTCCCA
    GGCAGTGTCA ACTCAGCTGT CCTCCAGCTG TCCCCATATG TCAACTACCA GTTCCGGGTC
    ATTGCCATCA ACGAGGTCGG CAGCAGCCAC CCCAGCCTCC CATCTGAGCG CTACCGAACC
    AGCGGAGCAC CCCCTGAGTC TAATCCCGCT GATGTGAAGG GAGAGGGGAC CAGAAAAAAC
    AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCCG CCTTTGGCCC CAACCTGCGC
    TACATCGTGA AGTGGCGGCG GAGAGAGACT CGAGAGAGCT GGAACAACGT CACAGTGTGG
    GGCTCCCGCT ACGTGGTGGG TCAGACCCCT GTCTATGTGC CCTACGAGAT CCGAGTCCAG
    GCCGAAAATG ACTTTGGGAA GGGCCCTGAG CCAGACACGG TCATCGGTTA CTCCGGAGAA
    GATTATCCCA GGGCTGCGCC CACCGACGTT AAAATCCGAG TGCTGAACAG CACGGCCATC
    AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCTGCTCAG AGAGTACCGA
    GCCTACTACT GGAGAGAGAG CAGCTTGCTG AAGAGCTTGT GGGTGTCTCA GAAGAGACAG
    CAGGCCAGCT TCCCTGGTGA CCGCCTCCGC GGCGTGGTGC CCCGCCTCTT CCCCTACAGC
    AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGCG ATGGGCCTCG CAGTGAGACC
    AAGGAGTTCA CAACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594997.2
    CDS47..3862
    Translation

    Target ORF information:

    RefSeq Version XM_014739511.2
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739511.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCAACCACC CCCATAATGA CTCGTCCTTA
    AGAAACCACC CTGACATGTA CAGTGCCCGA GGAGTTGCGG AAAGGACACC CAGCTTCATG
    TATCCCCAGG GCACTGCGAG CAGCCAGATG GTGCTGCGTG GCATGGACCT CCTGCTGGAG
    TGCATCGCCT CCGGGGTCCC GACACCAGAC ATCGCGTGGT ACAAGAAAGG TGGGGACCTC
    CCATCTGACA AGGCCAAGTT TGAGAACTTT AACAAGGCTC TACGTATCAC CAATGTCTCT
    GAGGAAGACT CTGGAGAGTA TTTCTGCCTG GCCTCCAACA AGATGGGCAG CATCCGGCAC
    ACGATCTCGG TGAGAGTAAA GGCTGCTCCC TACTGGCTGG ATGAACCCAA GAACCTTATC
    CTGGCTCCTG GCGAGGACGG GAGACTGGTG TGTCGAGCCA GTGGGAACCC CAAGCCCACC
    ATCCAGTGGA TGGTGAATGG GGAACCTTTG CAATCGGCAC CACCCAACCC AAACCGAGAG
    GTGGCCGGGG ACACCATCAT CTTCCGGGAC ACGCAGATCA GCAGCAGGGC CGTGTACCAG
    TGCAACACGT CCAACGAGCA CGGCTACCTG CTGGCCAACG CCTTCGTCAG CGTGCTGGAC
    GTGCCACCTC GGATGCTGTC ACCGCGGAAC CAGCTTATTA GGGTGATCCT GTACAACCGG
    ACGCGGCTGG ACTGCCCATT CTTTGGGTCC CCCATTCCCA CGCTCCGTTG GTTTAAGAAT
    GGACAAGGAA GCAACCTGGA TGGAGGCAAC TACCATGTCT ATGAGAATGG CAGTCTGGAA
    ATCAAGATGA TCCACAAAGA GGACCAAGGC ATCTACACCT GCGTTGCCAC TAACATCCTG
    GGCAAGGCAG AAAACCAGGT CCGCCTGGAA GTCAAAGACC CCACCAGGAT CTTTCGGATG
    CCTGAGGACC AGGTTGCCAA GAGGGGCACC ACGGTGCAGC TGGAGTGCCG GGTGAAGCAC
    GACCCCTCCC TGAAGCTCAC AGTGTCCTGG CTGAAGGATG ACGAGCCGCT CTACATCGGG
    AACAGGATGA AGAAGGAAGA TGACTCCCTG ACCATCTTTG GTGTGGCGGA GCGGGACCAG
    GGCAGTTACA CCTGCGTCGC CAGCACTGAG CTGGACCAAG ACCTGGCCAA GGCCTACCTC
    ACCGTGCTAG GACGGCCAGA CAGACCCCGG GACCTGGAGC TCACTGACCT GGCTGAGAGG
    AGTGTGAGGC TCACCTGGAT CCCAGGGGAT GACAACAATA GCCCCATCAC AGACTATGTC
    GTCCAGTTTG AAGAGGACCA GTTCCAGCCA GGGGTCTGGC ATGACCATTC CAAGTTCCCA
    GGCAGTGTCA ACTCAGCTGT CCTCCAGCTG TCCCCATATG TCAACTACCA GTTCCGGGTC
    ATTGCCATCA ACGAGGTCGG CAGCAGCCAC CCCAGCCTCC CATCTGAGCG CTACCGAACC
    AGCGGAGCAC CCCCTGAGTC TAATCCCGCT GATGTGAAGG GAGAGGGGAC CAGAAAAAAC
    AACATGGAGA TCACGTGGAC GCCCATGAAT GCCACCTCCG CCTTTGGCCC CAACCTGCGC
    TACATCGTGA AGTGGCGGCG GAGAGAGACT CGAGAGAGCT GGAACAACGT CACAGTGTGG
    GGCTCCCGCT ACGTGGTGGG TCAGACCCCT GTCTATGTGC CCTACGAGAT CCGAGTCCAG
    GCCGAAAATG ACTTTGGGAA GGGCCCTGAG CCAGACACGG TCATCGGTTA CTCCGGAGAA
    GATTATCCCA GGGCTGCGCC CACCGACGTT AAAATCCGAG TGCTGAACAG CACGGCCATC
    AGCCTTCAGT GGAACCGCGT CTACTCCGAC ACGGTCCAGG GCCTGCTCAG AGAGTACCGA
    GCCTACTACT GGAGAGAGAG CAGCTTGCTG AAGAGCTTGT GGGTGTCTCA GAAGAGACAG
    CAGGCCAGCT TCCCTGGTGA CCGCCTCCGC GGCGTGGTGC CCCGCCTCTT CCCCTACAGC
    AACTACAAGC TGGAGATGGT TGTGGTCAAT GGGAGAGGCG ATGGGCCTCG CAGTGAGACC
    AAGGAGTTCA CAACCCCAGA AGGAGTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCGAATGA AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64759
    Clone ID Related Accession (Same CDS sequence) XM_014739512.2
    Accession Version XM_014739512.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_014739512.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCGGA AAGGACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGCCAGATGG TGCTGCGTGG CATGGACCTC
    CTGCTGGAGT GCATCGCCTC CGGGGTCCCG ACACCAGACA TCGCGTGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTT GAGAACTTTA ACAAGGCTCT ACGTATCACC
    AATGTCTCTG AGGAAGACTC TGGAGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTTATCC TGGCTCCTGG CGAGGACGGG AGACTGGTGT GTCGAGCCAG TGGGAACCCC
    AAGCCCACCA TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCCAACCCA
    AACCGAGAGG TGGCCGGGGA CACCATCATC TTCCGGGACA CGCAGATCAG CAGCAGGGCC
    GTGTACCAGT GCAACACGTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCACCTCG GATGCTGTCA CCGCGGAACC AGCTTATTAG GGTGATCCTG
    TACAACCGGA CGCGGCTGGA CTGCCCATTC TTTGGGTCCC CCATTCCCAC GCTCCGTTGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGAGGCAACT ACCATGTCTA TGAGAATGGC
    AGTCTGGAAA TCAAGATGAT CCACAAAGAG GACCAAGGCA TCTACACCTG CGTTGCCACT
    AACATCCTGG GCAAGGCAGA AAACCAGGTC CGCCTGGAAG TCAAAGACCC CACCAGGATC
    TTTCGGATGC CTGAGGACCA GGTTGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGG
    GTGAAGCACG ACCCCTCCCT GAAGCTCACA GTGTCCTGGC TGAAGGATGA CGAGCCGCTC
    TACATCGGGA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CCATCTTTGG TGTGGCGGAG
    CGGGACCAGG GCAGTTACAC CTGCGTCGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAG
    GCCTACCTCA CCGTGCTAGG ACGGCCAGAC AGACCCCGGG ACCTGGAGCT CACTGACCTG
    GCTGAGAGGA GTGTGAGGCT CACCTGGATC CCAGGGGATG ACAACAATAG CCCCATCACA
    GACTATGTCG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCTGTC CTCCAGCTGT CCCCATATGT CAACTACCAG
    TTCCGGGTCA TTGCCATCAA CGAGGTCGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCTGAGTCT AATCCCGCTG ATGTGAAGGG AGAGGGGACC
    AGAAAAAACA ACATGGAGAT CACGTGGACG CCCATGAATG CCACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATCGTGAA GTGGCGGCGG AGAGAGACTC GAGAGAGCTG GAACAACGTC
    ACAGTGTGGG GCTCCCGCTA CGTGGTGGGT CAGACCCCTG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CCGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACGGT CATCGGTTAC
    TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACCGACGTTA AAATCCGAGT GCTGAACAGC
    ACGGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCTGCTCAGA
    GAGTACCGAG CCTACTACTG GAGAGAGAGC AGCTTGCTGA AGAGCTTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGCCTCCGCG GCGTGGTGCC CCGCCTCTTC
    CCCTACAGCA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGCGA TGGGCCTCGC
    AGTGAGACCA AGGAGTTCAC AACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGATTCCGA
    GTCCGGCAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ACCATCCGGA ACACCCCAAT
    GGGATCCTGA CTGGATACAC CCTCAGATAC GTGCCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG GGAAGCTGTC
    ACAGAGGAGT CGCCAGCACC CCCGAATGAA GCTACTCCAA CCGCAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594998.2
    CDS47..3826
    Translation

    Target ORF information:

    RefSeq Version XM_014739512.2
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739512.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCTGT CCCAGCCTCC GACCATCACC
    AAGCAGTCGG TGAAGGACCA CATCGTGGAT CCCCGGGATA ACATCCTGAT TGAGTGTGAG
    GCGAAGGGGA ACCCCGCCCC CAGCTTCCAC TGGACTCGCA ACAGCAGGTT CTTCAACATC
    GCCAAGGACC CCCGGGTGTC CATGAAGAGG CGGTCTGGGA CCCTGGTGAT CGACTTCCGC
    AGCGGCGGGC GGCCAGAGGA GTACGAGGGG GAATACCAGT GCTTCGCCCG CAACAAGTTC
    GGCACCGCCC TGTCCAACAG GATCCGCCTG CAGGTGTCCA AATCTCCCCT GTGGCCCAAG
    GAAAACCTAG ACCCTGTCGT GGTCCAAGAA GGCGCCCCCT TGACACTCCA GTGTAACCCC
    CCGCCTGGAC TTCCGTCCCC AGTCATCTTC TGGATGAGCA GCACCATGGA CCCCATCACC
    CAAGACAAGC GTGTCTCCCA GGGCCACAAC GGAGACCTGT ATTTCTCCAA CGTCATGCTG
    CAGGACATGC AGACTGACTA CAGCTGCAAT GCCCGCTTCC ACTTTACCCA CACCATCCAG
    CAGAAGAACG CGTTCACCCT CAAGGTCCTC ACCACCCGAG GAGTTGCGGA AAGGACACCC
    AGCTTCATGT ATCCCCAGGG CACTGCGAGC AGCCAGATGG TGCTGCGTGG CATGGACCTC
    CTGCTGGAGT GCATCGCCTC CGGGGTCCCG ACACCAGACA TCGCGTGGTA CAAGAAAGGT
    GGGGACCTCC CATCTGACAA GGCCAAGTTT GAGAACTTTA ACAAGGCTCT ACGTATCACC
    AATGTCTCTG AGGAAGACTC TGGAGAGTAT TTCTGCCTGG CCTCCAACAA GATGGGCAGC
    ATCCGGCACA CGATCTCGGT GAGAGTAAAG GCTGCTCCCT ACTGGCTGGA TGAACCCAAG
    AACCTTATCC TGGCTCCTGG CGAGGACGGG AGACTGGTGT GTCGAGCCAG TGGGAACCCC
    AAGCCCACCA TCCAGTGGAT GGTGAATGGG GAACCTTTGC AATCGGCACC ACCCAACCCA
    AACCGAGAGG TGGCCGGGGA CACCATCATC TTCCGGGACA CGCAGATCAG CAGCAGGGCC
    GTGTACCAGT GCAACACGTC CAACGAGCAC GGCTACCTGC TGGCCAACGC CTTCGTCAGC
    GTGCTGGACG TGCCACCTCG GATGCTGTCA CCGCGGAACC AGCTTATTAG GGTGATCCTG
    TACAACCGGA CGCGGCTGGA CTGCCCATTC TTTGGGTCCC CCATTCCCAC GCTCCGTTGG
    TTTAAGAATG GACAAGGAAG CAACCTGGAT GGAGGCAACT ACCATGTCTA TGAGAATGGC
    AGTCTGGAAA TCAAGATGAT CCACAAAGAG GACCAAGGCA TCTACACCTG CGTTGCCACT
    AACATCCTGG GCAAGGCAGA AAACCAGGTC CGCCTGGAAG TCAAAGACCC CACCAGGATC
    TTTCGGATGC CTGAGGACCA GGTTGCCAAG AGGGGCACCA CGGTGCAGCT GGAGTGCCGG
    GTGAAGCACG ACCCCTCCCT GAAGCTCACA GTGTCCTGGC TGAAGGATGA CGAGCCGCTC
    TACATCGGGA ACAGGATGAA GAAGGAAGAT GACTCCCTGA CCATCTTTGG TGTGGCGGAG
    CGGGACCAGG GCAGTTACAC CTGCGTCGCC AGCACTGAGC TGGACCAAGA CCTGGCCAAG
    GCCTACCTCA CCGTGCTAGG ACGGCCAGAC AGACCCCGGG ACCTGGAGCT CACTGACCTG
    GCTGAGAGGA GTGTGAGGCT CACCTGGATC CCAGGGGATG ACAACAATAG CCCCATCACA
    GACTATGTCG TCCAGTTTGA AGAGGACCAG TTCCAGCCAG GGGTCTGGCA TGACCATTCC
    AAGTTCCCAG GCAGTGTCAA CTCAGCTGTC CTCCAGCTGT CCCCATATGT CAACTACCAG
    TTCCGGGTCA TTGCCATCAA CGAGGTCGGC AGCAGCCACC CCAGCCTCCC ATCTGAGCGC
    TACCGAACCA GCGGAGCACC CCCTGAGTCT AATCCCGCTG ATGTGAAGGG AGAGGGGACC
    AGAAAAAACA ACATGGAGAT CACGTGGACG CCCATGAATG CCACCTCCGC CTTTGGCCCC
    AACCTGCGCT ACATCGTGAA GTGGCGGCGG AGAGAGACTC GAGAGAGCTG GAACAACGTC
    ACAGTGTGGG GCTCCCGCTA CGTGGTGGGT CAGACCCCTG TCTATGTGCC CTACGAGATC
    CGAGTCCAGG CCGAAAATGA CTTTGGGAAG GGCCCTGAGC CAGACACGGT CATCGGTTAC
    TCCGGAGAAG ATTATCCCAG GGCTGCGCCC ACCGACGTTA AAATCCGAGT GCTGAACAGC
    ACGGCCATCA GCCTTCAGTG GAACCGCGTC TACTCCGACA CGGTCCAGGG CCTGCTCAGA
    GAGTACCGAG CCTACTACTG GAGAGAGAGC AGCTTGCTGA AGAGCTTGTG GGTGTCTCAG
    AAGAGACAGC AGGCCAGCTT CCCTGGTGAC CGCCTCCGCG GCGTGGTGCC CCGCCTCTTC
    CCCTACAGCA ACTACAAGCT GGAGATGGTT GTGGTCAATG GGAGAGGCGA TGGGCCTCGC
    AGTGAGACCA AGGAGTTCAC AACCCCAGAA GGAGTACCCA GTGCCCCCAG GCGATTCCGA
    GTCCGGCAGC CCAACCTGGA GACCATCAAC CTGGAATGGG ACCATCCGGA ACACCCCAAT
    GGGATCCTGA CTGGATACAC CCTCAGATAC GTGCCCTTTA ATGGAACCAA AGTAGGAAAG
    CAGATAGTGG AAAACTTCTC TCCCAATCAG ACCAAGTTCA CGGTGCAAAG AGCAGACCCC
    GTGTCGCGCT ACCGCTTTAC CCTCAGTGCC AGGACGCAGG TGGGCTCTGG GGAAGCTGTC
    ACAGAGGAGT CGCCAGCACC CCCGAATGAA GCTACTCCAA CCGCAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64760
    Clone ID Related Accession (Same CDS sequence) XM_023640375.1
    Accession Version XM_023640375.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACTACAGT GGGTACTGTG
    AGCAGTACTG ATGCTACTGT CACTGCTGCC ACCACTGAAG CCACAACAGT CCCCATCATC
    CCAACTGTCG CACCTGCCAC CATCGCTACC ACCACCGTCG CCACAACTAC TACAACCACT
    GCCGCCACCA CCACCACCAC CACGGAGGGT CCTCCCACCA CCACCACCGG GACTAAGATA
    CACGAATCCG CCCCGGACGA GCAGTCCATA TGGAACGTCA CAGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAAGTGTC CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGAG TCTATTCCCG GGACAACGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496143.1
    CDS49..3822
    Translation

    Target ORF information:

    RefSeq Version XM_023640375.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640375.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCTCCTCCC ACGTTGCCCC CGACTACAGT GGGTACTGTG
    AGCAGTACTG ATGCTACTGT CACTGCTGCC ACCACTGAAG CCACAACAGT CCCCATCATC
    CCAACTGTCG CACCTGCCAC CATCGCTACC ACCACCGTCG CCACAACTAC TACAACCACT
    GCCGCCACCA CCACCACCAC CACGGAGGGT CCTCCCACCA CCACCACCGG GACTAAGATA
    CACGAATCCG CCCCGGACGA GCAGTCCATA TGGAACGTCA CAGTGCTCCC CAACAGTAAA
    TGGGCCAACA TCACCTGGAA GCACAATTTC GGGCCCGGAA CTGACTTTGT GGTTGAGTAC
    ATCGACAGCA ACCATACGAA AAAAAGTGTC CCTGTTAAGG CCCAGGCGCA GCCTGTCCAG
    CTGACAGACC TCTATCCCGG GATGACATAC ACGTTGCGAG TCTATTCCCG GGACAACGAG
    GGCATCAGCA GTACCGTCAT CACCTTTATG ACCAGTACAG CTTACACCAA CAACCAGGCG
    GACATCGCCA CCCAGGGCTG GTTCATCGGG CTCATGTGCG CCATCGCCCT CCTGGTGCTG
    ATTCTGCTCA TCGTCTGTTT CATCAAGAGG AGTCGAGGCG GCAAGTACCC AGTGCGAGAG
    AAGAAGGACG TTCCCCTCGG CCCTGAAGAC CCCAAAGAAG AGGACGGCTC CTTTGACTAC
    AGTGATGAGG ACAACAAGCC CCTGCAGGGC AGCCAGACAT CCCTGGATGG CACCATCAAG
    CAGCAGGAGA GTGACGACAG CCTGGTGGAC TACGGCGAGG GCGGCGAGGG GCAGTTCAAT
    GAGGACGGCT CCTTTATCGG CCAGTACACA GTCAAAAAGG ACAAGGAGGA GACAGAGGGC
    AATGAAAGCT CAGAGGCCAC GTCACCTGTC AACGCCATCT ATTCTCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64761
    Clone ID Related Accession (Same CDS sequence) XM_023640376.1
    Accession Version XM_023640376.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3558bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTTAC CCAGTGCCCC CAGGCGATTC CGAGTCCGGC AGCCCAACCT GGAGACCATC
    AACCTGGAAT GGGACCATCC GGAACACCCC AATGGGATCC TGACTGGATA CACCCTCAGA
    TACGTGCCCT TTAATGGAAC CAAAGTAGGA AAGCAGATAG TGGAAAACTT CTCTCCCAAT
    CAGACCAAGT TCACGGTGCA AAGAGCAGAC CCCGTGTCGC GCTACCGCTT TACCCTCAGT
    GCCAGGACGC AGGTGGGCTC TGGGGAAGCT GTCACAGAGG AGTCGCCAGC ACCCCCGAAT
    GAAGCTTACA CCAACAACCA GGCGGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG
    TGCGCCATCG CCCTCCTGGT GCTGATTCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGA
    GGCGGCAAGT ACCCAGTGCG AGAGAAGAAG GACGTTCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGACG GCTCCTTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACATCCCTGG ATGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTGGT GGACTACGGC
    GAGGGCGGCG AGGGGCAGTT CAATGAGGAC GGCTCCTTTA TCGGCCAGTA CACAGTCAAA
    AAGGACAAGG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACGTCACC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496144.1
    CDS46..3603
    Translation

    Target ORF information:

    RefSeq Version XM_023640376.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640376.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTTAC CCAGTGCCCC CAGGCGATTC CGAGTCCGGC AGCCCAACCT GGAGACCATC
    AACCTGGAAT GGGACCATCC GGAACACCCC AATGGGATCC TGACTGGATA CACCCTCAGA
    TACGTGCCCT TTAATGGAAC CAAAGTAGGA AAGCAGATAG TGGAAAACTT CTCTCCCAAT
    CAGACCAAGT TCACGGTGCA AAGAGCAGAC CCCGTGTCGC GCTACCGCTT TACCCTCAGT
    GCCAGGACGC AGGTGGGCTC TGGGGAAGCT GTCACAGAGG AGTCGCCAGC ACCCCCGAAT
    GAAGCTTACA CCAACAACCA GGCGGACATC GCCACCCAGG GCTGGTTCAT CGGGCTCATG
    TGCGCCATCG CCCTCCTGGT GCTGATTCTG CTCATCGTCT GTTTCATCAA GAGGAGTCGA
    GGCGGCAAGT ACCCAGTGCG AGAGAAGAAG GACGTTCCCC TCGGCCCTGA AGACCCCAAA
    GAAGAGGACG GCTCCTTTGA CTACAGTGAT GAGGACAACA AGCCCCTGCA GGGCAGCCAG
    ACATCCCTGG ATGGCACCAT CAAGCAGCAG GAGAGTGACG ACAGCCTGGT GGACTACGGC
    GAGGGCGGCG AGGGGCAGTT CAATGAGGAC GGCTCCTTTA TCGGCCAGTA CACAGTCAAA
    AAGGACAAGG AGGAGACAGA GGGCAATGAA AGCTCAGAGG CCACGTCACC TGTCAACGCC
    ATCTATTCTC TGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64762
    Clone ID Related Accession (Same CDS sequence) XM_023640377.1
    Accession Version XM_023640377.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496145.1
    CDS48..3593
    Translation

    Target ORF information:

    RefSeq Version XM_023640377.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640377.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64763
    Clone ID Related Accession (Same CDS sequence) XM_023640379.1 , XM_023640378.1 , XM_023640381.1 , XM_023640380.1 , XM_023640382.1
    Accession Version XM_023640379.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496147.1
    CDS483..4568
    Translation

    Target ORF information:

    RefSeq Version XM_023640379.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640379.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64763
    Clone ID Related Accession (Same CDS sequence) XM_023640379.1 , XM_023640378.1 , XM_023640381.1 , XM_023640380.1 , XM_023640382.1
    Accession Version XM_023640378.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496146.1
    CDS339..4424
    Translation

    Target ORF information:

    RefSeq Version XM_023640378.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640378.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64763
    Clone ID Related Accession (Same CDS sequence) XM_023640379.1 , XM_023640378.1 , XM_023640381.1 , XM_023640380.1 , XM_023640382.1
    Accession Version XM_023640381.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496149.1
    CDS418..4503
    Translation

    Target ORF information:

    RefSeq Version XM_023640381.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640381.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64763
    Clone ID Related Accession (Same CDS sequence) XM_023640379.1 , XM_023640378.1 , XM_023640381.1 , XM_023640380.1 , XM_023640382.1
    Accession Version XM_023640380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496148.1
    CDS1648..5733
    Translation

    Target ORF information:

    RefSeq Version XM_023640380.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64763
    Clone ID Related Accession (Same CDS sequence) XM_023640379.1 , XM_023640378.1 , XM_023640381.1 , XM_023640380.1 , XM_023640382.1
    Accession Version XM_023640382.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4086bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496150.1
    CDS106..4191
    Translation

    Target ORF information:

    RefSeq Version XM_023640382.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640382.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG ACTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAGAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGCTGATCAG GCCACTCCAA CTAACCGTCT GGCTGCCCTG
    CCCAAAGGAC GGCCAGACAG ACCCCGGGAC CTGGAGCTCA CTGACCTGGC TGAGAGGAGT
    GTGAGGCTCA CCTGGATCCC AGGGGATGAC AACAATAGCC CCATCACAGA CTATGTCGTC
    CAGTTTGAAG AGGACCAGTT CCAGCCAGGG GTCTGGCATG ACCATTCCAA GTTCCCAGGC
    AGTGTCAACT CAGCTGTCCT CCAGCTGTCC CCATATGTCA ACTACCAGTT CCGGGTCATT
    GCCATCAACG AGGTCGGCAG CAGCCACCCC AGCCTCCCAT CTGAGCGCTA CCGAACCAGC
    GGAGCACCCC CTGAGTCTAA TCCCGCTGAT GTGAAGGGAG AGGGGACCAG AAAAAACAAC
    ATGGAGATCA CGTGGACGCC CATGAATGCC ACCTCCGCCT TTGGCCCCAA CCTGCGCTAC
    ATCGTGAAGT GGCGGCGGAG AGAGACTCGA GAGAGCTGGA ACAACGTCAC AGTGTGGGGC
    TCCCGCTACG TGGTGGGTCA GACCCCTGTC TATGTGCCCT ACGAGATCCG AGTCCAGGCC
    GAAAATGACT TTGGGAAGGG CCCTGAGCCA GACACGGTCA TCGGTTACTC CGGAGAAGAT
    TATCCCAGGG CTGCGCCCAC CGACGTTAAA ATCCGAGTGC TGAACAGCAC GGCCATCAGC
    CTTCAGTGGA ACCGCGTCTA CTCCGACACG GTCCAGGGCC TGCTCAGAGA GTACCGAGCC
    TACTACTGGA GAGAGAGCAG CTTGCTGAAG AGCTTGTGGG TGTCTCAGAA GAGACAGCAG
    GCCAGCTTCC CTGGTGACCG CCTCCGCGGC GTGGTGCCCC GCCTCTTCCC CTACAGCAAC
    TACAAGCTGG AGATGGTTGT GGTCAATGGG AGAGGCGATG GGCCTCGCAG TGAGACCAAG
    GAGTTCACAA CCCCAGAAGG AGTACCCAGT GCCCCCAGGC GATTCCGAGT CCGGCAGCCC
    AACCTGGAGA CCATCAACCT GGAATGGGAC CATCCGGAAC ACCCCAATGG GATCCTGACT
    GGATACACCC TCAGATACGT GCCCTTTAAT GGAACCAAAG TAGGAAAGCA GATAGTGGAA
    AACTTCTCTC CCAATCAGAC CAAGTTCACG GTGCAAAGAG CAGACCCCGT GTCGCGCTAC
    CGCTTTACCC TCAGTGCCAG GACGCAGGTG GGCTCTGGGG AAGCTGTCAC AGAGGAGTCG
    CCAGCACCCC CGAATGAAGC TACTCCAACC GCAGCTCCTC CCACGTTGCC CCCGACTACA
    GTGGGTACTG TGAGCAGTAC TGATGCTACT GTCACTGCTG CCACCACTGA AGCCACAACA
    GTCCCCATCA TCCCAACTGT CGCACCTGCC ACCATCGCTA CCACCACCGT CGCCACAACT
    ACTACAACCA CTGCCGCCAC CACCACCACC ACCACGGAGG GTCCTCCCAC CACCACCACC
    GGGACTAAGA TACACGAATC CGCCCCGGAC GAGCAGTCCA TATGGAACGT CACAGTGCTC
    CCCAACAGTA AATGGGCCAA CATCACCTGG AAGCACAATT TCGGGCCCGG AACTGACTTT
    GTGGTTGAGT ACATCGACAG CAACCATACG AAAAAAAGTG TCCCTGTTAA GGCCCAGGCG
    CAGCCTGTCC AGCTGACAGA CCTCTATCCC GGGATGACAT ACACGTTGCG AGTCTATTCC
    CGGGACAACG AGGGCATCAG CAGTACCGTC ATCACCTTTA TGACCAGTAC AGCTTACACC
    AACAACCAGG CGGACATCGC CACCCAGGGC TGGTTCATCG GGCTCATGTG CGCCATCGCC
    CTCCTGGTGC TGATTCTGCT CATCGTCTGT TTCATCAAGA GGAGTCGAGG CGGCAAGTAC
    CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC GGCCCTGAAG ACCCCAAAGA AGAGGACGGC
    TCCTTTGACT ACAGTGATGA GGACAACAAG CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT
    GGCACCATCA AGCAGCAGGA GAGTGACGAC AGCCTGGTGG ACTACGGCGA GGGCGGCGAG
    GGGCAGTTCA ATGAGGACGG CTCCTTTATC GGCCAGTACA CAGTCAAAAA GGACAAGGAG
    GAGACAGAGG GCAATGAAAG CTCAGAGGCC ACGTCACCTG TCAACGCCAT CTATTCTCTG
    GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64764
    Clone ID Related Accession (Same CDS sequence) XM_023640383.1
    Accession Version XM_023640383.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3276bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496151.1
    CDS46..3321
    Translation

    Target ORF information:

    RefSeq Version XM_023640383.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640383.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTA CTCCAACCGC AGCTTACACC AACAACCAGG CGGACATCGC CACCCAGGGC
    TGGTTCATCG GGCTCATGTG CGCCATCGCC CTCCTGGTGC TGATTCTGCT CATCGTCTGT
    TTCATCAAGA GGAGTCGAGG CGGCAAGTAC CCAGTGCGAG AGAAGAAGGA CGTTCCCCTC
    GGCCCTGAAG ACCCCAAAGA AGAGGACGGC TCCTTTGACT ACAGTGATGA GGACAACAAG
    CCCCTGCAGG GCAGCCAGAC ATCCCTGGAT GGCACCATCA AGCAGCAGGA GAGTGACGAC
    AGCCTGGTGG ACTACGGCGA GGGCGGCGAG GGGCAGTTCA ATGAGGACGG CTCCTTTATC
    GGCCAGTACA CAGTCAAAAA GGACAAGGAG GAGACAGAGG GCAATGAAAG CTCAGAGGCC
    ACGTCACCTG TCAACGCCAT CTATTCTCTG GCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb64765
    Clone ID Related Accession (Same CDS sequence) XM_023640384.1
    Accession Version XM_023640384.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product neurofascin isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009148.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023496152.1
    CDS46..3306
    Translation

    Target ORF information:

    RefSeq Version XM_023640384.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023640384.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGGCGGC TGGCGGCGAG AGGCGCTGCA GGGGACGCGG GGGAAGTGGC GGCGCCGGCC 
    GCGGACAGCA CGCACTGCGC CCAGGGCCGG AGCCCTAGCC CTCCGAGGTT GATTGACGTA
    TGTGCCATTT GGGATACTGG AGTTCTCCCT CCCACCGCAG ACTGGAAGGG AGCCTCCTCG
    GGGGTTGCAG GGGCGAGGCT GAATGAGGCA GAGACGCTGA GCTCTATCCA GGAGGCCCAG
    TTAGAACGGC TGGAGGTGGC CACACCCTGC GGGCCGGGGA GCAGGGAGCG GGGCCCCGTG
    CTGAGGATGG CCAGGCAGCA GCTGCCACCC TGGGTCCATG CAGCCATCCT CCTCTGCCTC
    CTCAGCCTCG GCCGGGCCAT CGAGATTCCT ATGGATCCAA GCATTCAGAA CGAGCTGTCC
    CAGCCTCCGA CCATCACCAA GCAGTCGGTG AAGGACCACA TCGTGGATCC CCGGGATAAC
    ATCCTGATTG AGTGTGAGGC GAAGGGGAAC CCCGCCCCCA GCTTCCACTG GACTCGCAAC
    AGCAGGTTCT TCAACATCGC CAAGGACCCC CGGGTGTCCA TGAAGAGGCG GTCTGGGACC
    CTGGTGATCG ACTTCCGCAG CGGCGGGCGG CCAGAGGAGT ACGAGGGGGA ATACCAGTGC
    TTCGCCCGCA ACAAGTTCGG CACCGCCCTG TCCAACAGGA TCCGCCTGCA GGTGTCCAAA
    TCTCCCCTGT GGCCCAAGGA AAACCTAGAC CCTGTCGTGG TCCAAGAAGG CGCCCCCTTG
    ACACTCCAGT GTAACCCCCC GCCTGGACTT CCGTCCCCAG TCATCTTCTG GATGAGCAGC
    ACCATGGACC CCATCACCCA AGACAAGCGT GTCTCCCAGG GCCACAACGG AGACCTGTAT
    TTCTCCAACG TCATGCTGCA GGACATGCAG ACTGACTACA GCTGCAATGC CCGCTTCCAC
    TTTACCCACA CCATCCAGCA GAAGAACGCG TTCACCCTCA AGGTCCTCAC CAACCACCCC
    CATAATGACT CGTCCTTAAG AAACCACCCT GACATGTACA GTGCCCGAGG AGTTGCGGAA
    AGGACACCCA GCTTCATGTA TCCCCAGGGC ACTGCGAGCA GCCAGATGGT GCTGCGTGGC
    ATGGACCTCC TGCTGGAGTG CATCGCCTCC GGGGTCCCGA CACCAGACAT CGCGTGGTAC
    AAGAAAGGTG GGGACCTCCC ATCTGACAAG GCCAAGTTTG AGAACTTTAA CAAGGCTCTA
    CGTATCACCA ATGTCTCTGA GGAAGACTCT GGAGAGTATT TCTGCCTGGC CTCCAACAAG
    ATGGGCAGCA TCCGGCACAC GATCTCGGTG AGAGTAAAGG CTGCTCCCTA CTGGCTGGAT
    GAACCCAAGA ACCTTATCCT GGCTCCTGGC GAGGACGGGA GACTGGTGTG TCGAGCCAGT
    GGGAACCCCA AGCCCACCAT CCAGTGGATG GTGAATGGGG AACCTTTGCA ATCGGCACCA
    CCCAACCCAA ACCGAGAGGT GGCCGGGGAC ACCATCATCT TCCGGGACAC GCAGATCAGC
    AGCAGGGCCG TGTACCAGTG CAACACGTCC AACGAGCACG GCTACCTGCT GGCCAACGCC
    TTCGTCAGCG TGCTGGACGT GCCACCTCGG ATGCTGTCAC CGCGGAACCA GCTTATTAGG
    GTGATCCTGT ACAACCGGAC GCGGCTGGAC TGCCCATTCT TTGGGTCCCC CATTCCCACG
    CTCCGTTGGT TTAAGAATGG ACAAGGAAGC AACCTGGATG GAGGCAACTA CCATGTCTAT
    GAGAATGGCA GTCTGGAAAT CAAGATGATC CACAAAGAGG ACCAAGGCAT CTACACCTGC
    GTTGCCACTA ACATCCTGGG CAAGGCAGAA AACCAGGTCC GCCTGGAAGT CAAAGACCCC
    ACCAGGATCT TTCGGATGCC TGAGGACCAG GTTGCCAAGA GGGGCACCAC GGTGCAGCTG
    GAGTGCCGGG TGAAGCACGA CCCCTCCCTG AAGCTCACAG TGTCCTGGCT GAAGGATGAC
    GAGCCGCTCT ACATCGGGAA CAGGATGAAG AAGGAAGATG ACTCCCTGAC CATCTTTGGT
    GTGGCGGAGC GGGACCAGGG CAGTTACACC TGCGTCGCCA GCACTGAGCT GGACCAAGAC
    CTGGCCAAGG CCTACCTCAC CGTGCTAGCT GATCAGGCCA CTCCAACTAA CCGTCTGGCT
    GCCCTGCCCA AAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb20196
    Clone ID Related Accession (Same CDS sequence) XM_014739503.1
    Accession Version XM_014739503.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CGTTGCCCCC GACTACAGTG
    GGTACTGTGA GCAGTACTGA TGCTACTGTC ACTGCTGCCA CCACTGAAGC CACAACAGTC
    CCCATCATCC CAACTGTCGC ACCTGCCACC ATCGCTACCA CCACCGTCGC CACAACTACT
    ACAACCACTG CCGCCACCAC CACCACCACC ACGGAGGGTC CTCCCACCAC CACCACCGGG
    ACTAAGATAC ACGAATCCGC CCCGGACGAG CAGTCCATAT GGAACGTCAC AGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAAGTGTCC CTGTTAAGGC CCAGGCGCAG
    CCTGTCCAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGAGT CTATTCCCGG
    GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC
    AACCAGGCGG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGCGC CATCGCCCTC
    CTGGTGCTGA TTCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGAGGCGG CAAGTACCCA
    GTGCGAGAGA AGAAGGACGT TCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGACGGCTCC
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC CCTGGATGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ACGGCGAGGG CGGCGAGGGG
    CAGTTCAATG AGGACGGCTC CTTTATCGGC CAGTACACAG TCAAAAAGGA CAAGGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACG TCACCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594989.1
    CDS196..4218
    Translation

    Target ORF information:

    RefSeq Version XM_014739503.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739503.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCAAC CACCCCCATA ATGACTCGTC CTTAAGAAAC
    CACCCTGACA TGTACAGTGC CCGAGGAGTT GCGGAAAGGA CACCCAGCTT CATGTATCCC
    CAGGGCACTG CGAGCAGCCA GATGGTGCTG CGTGGCATGG ACCTCCTGCT GGAGTGCATC
    GCCTCCGGGG TCCCGACACC AGACATCGCG TGGTACAAGA AAGGTGGGGA CCTCCCATCT
    GACAAGGCCA AGTTTGAGAA CTTTAACAAG GCTCTACGTA TCACCAATGT CTCTGAGGAA
    GACTCTGGAG AGTATTTCTG CCTGGCCTCC AACAAGATGG GCAGCATCCG GCACACGATC
    TCGGTGAGAG TAAAGGCTGC TCCCTACTGG CTGGATGAAC CCAAGAACCT TATCCTGGCT
    CCTGGCGAGG ACGGGAGACT GGTGTGTCGA GCCAGTGGGA ACCCCAAGCC CACCATCCAG
    TGGATGGTGA ATGGGGAACC TTTGCAATCG GCACCACCCA ACCCAAACCG AGAGGTGGCC
    GGGGACACCA TCATCTTCCG GGACACGCAA ATCAGCAGCA GGGCCGTGTA CCAGTGCAAC
    ACGTCCAACG AGCACGGCTA CCTGCTGGCC AACGCCTTCG TCAGCGTGCT GGACGTGCCA
    CCTCGGATGC TGTCACCGCG GAACCAGCTT ATTAGGGTGA TCCTGTACAA CCGGACGCGG
    CTGGACTGCC CATTCTTTGG GTCCCCCATT CCCACGCTCC GTTGGTTTAA GAATGGACAA
    GGAAGCAACC TGGATGGAGG CAACTACCAT GTCTATGAGA ATGGCAGTCT GGAAATCAAG
    ATGATCCACA AAGAGGACCA AGGCATCTAC ACCTGCGTTG CCACTAACAT CCTGGGCAAG
    GCAGAAAACC AGGTCCGCCT GGAAGTCAAA GACCCCACCA GGATCTTTCG GATGCCTGAG
    GACCAGGTTG CCAAGAGGGG CACCACGGTG CAGCTGGAGT GCCGGGTGAA GCACGACCCC
    TCCCTGAAGC TCACAGTGTC CTGGCTGAAG GATGACGAGC CGCTCTACAT CGGGAACAGG
    ATGAAGAAGG AAGATGACTC CCTGACCATC TTTGGTGTGG CGGAGCGGGA CCAGGGCAGT
    TACACCTGCG TCGCCAGCAC TGAGCTGGAC CAAGACCTGG CCAAGGCCTA CCTCACCGTG
    CTAGGACGGC CAGACAGACC CCGGGACCTG GAGCTCACTG ACCTGGCTGA GAGGAGTGTG
    AGGCTCACCT GGATCCCAGG GGATGACAAC AATAGCCCCA TCACAGACTA TGTCGTCCAG
    TTTGAAGAGG ACCAGTTCCA GCCAGGGGTC TGGCATGACC ATTCCAAGTT CCCAGGCAGT
    GTCAACTCAG CTGTCCTCCA GCTGTCCCCA TATGTCAACT ACCAGTTCCG GGTCATTGCC
    ATCAACGAGG TCGGCAGCAG CCACCCCAGC CTCCCATCTG AGCGCTACCG AACCAGCGGA
    GCACCCCCTG AGTCTAATCC CGCTGATGTG AAGGGAGAGG GGACCAGAAA AAACAACATG
    GAGATCACGT GGACGCCCAT GAATGCCACC TCCGCCTTTG GCCCCAACCT GCGCTACATC
    GTGAAGTGGC GGCGGAGAGA GACTCGAGAG AGCTGGAACA ACGTCACAGT GTGGGGCTCC
    CGCTACGTGG TGGGTCAGAC CCCTGTCTAT GTGCCCTACG AGATCCGAGT CCAGGCCGAA
    AATGACTTTG GGAAGGGCCC TGAGCCAGAC ACGGTCATCG GTTACTCCGG AGAAGATTAT
    CCCAGGGCTG CGCCCACCGA CGTTAAAATC CGAGTGCTGA ACAGCACGGC CATCAGCCTT
    CAGTGGAACC GCGTCTACTC CGACACGGTC CAGGGCCTGC TCAGAGAGTA CCGAGCCTAC
    TACTGGAGAG AGAGCAGCTT GCTGAAGAGC TTGTGGGTGT CTCAGAAGAG ACAGCAGGCC
    AGCTTCCCTG GTGACCGCCT CCGCGGCGTG GTGCCCCGCC TCTTCCCCTA CAGCAACTAC
    AAGCTGGAGA TGGTTGTGGT CAATGGGAGA GGCGATGGGC CTCGCAGTGA GACCAAGGAG
    TTCACAACCC CAGAAGGAGT ACCCAGTGCC CCCAGGCGAT TCCGAGTCCG GCAGCCCAAC
    CTGGAGACCA TCAACCTGGA ATGGGACCAT CCGGAACACC CCAATGGGAT CCTGACTGGA
    TACACCCTCA GATACGTGCC CTTTAATGGA ACCAAAGTAG GAAAGCAGAT AGTGGAAAAC
    TTCTCTCCCA ATCAGACCAA GTTCACGGTG CAAAGAGCAG ACCCCGTGTC GCGCTACCGC
    TTTACCCTCA GTGCCAGGAC GCAGGTGGGC TCTGGGGAAG CTGTCACAGA GGAGTCGCCA
    GCACCCCCAA ATGAAGCTAC TCCAACCGCA GCTCCTCCCA CGTTGCCCCC GACTACAGTG
    GGTACTGTGA GCAGTACTGA TGCTACTGTC ACTGCTGCCA CCACTGAAGC CACAACAGTC
    CCCATCATCC CAACTGTCGC ACCTGCCACC ATCGCTACCA CCACCGTCGC CACAACTACT
    ACAACCACTG CCGCCACCAC CACCACCACC ACGGAGGGTC CTCCCACCAC CACCACCGGG
    ACTAAGATAC ACGAATCCGC CCCGGACGAG CAGTCCATAT GGAACGTCAC AGTGCTCCCC
    AACAGTAAAT GGGCCAACAT CACCTGGAAG CACAATTTCG GGCCCGGAAC TGACTTTGTG
    GTTGAGTACA TCGACAGCAA CCATACGAAA AAAAGTGTCC CTGTTAAGGC CCAGGCGCAG
    CCTGTCCAGC TGACAGACCT CTATCCCGGG ATGACATACA CGTTGCGAGT CTATTCCCGG
    GACAACGAGG GCATCAGCAG TACCGTCATC ACCTTTATGA CCAGTACAGC TTACACCAAC
    AACCAGGCGG ACATCGCCAC CCAGGGCTGG TTCATCGGGC TCATGTGCGC CATCGCCCTC
    CTGGTGCTGA TTCTGCTCAT CGTCTGTTTC ATCAAGAGGA GTCGAGGCGG CAAGTACCCA
    GTGCGAGAGA AGAAGGACGT TCCCCTCGGC CCTGAAGACC CCAAAGAAGA GGACGGCTCC
    TTTGACTACA GTGATGAGGA CAACAAGCCC CTGCAGGGCA GCCAGACATC CCTGGATGGC
    ACCATCAAGC AGCAGGAGAG TGACGACAGC CTGGTGGACT ACGGCGAGGG CGGCGAGGGG
    CAGTTCAATG AGGACGGCTC CTTTATCGGC CAGTACACAG TCAAAAAGGA CAAGGAGGAG
    ACAGAGGGCA ATGAAAGCTC AGAGGCCACG TCACCTGTCA ACGCCATCTA TTCTCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb20197
    Clone ID Related Accession (Same CDS sequence) XM_014739515.1
    Accession Version XM_014739515.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTACTCCA ACCGCAGCTC CTCCCACGTT GCCCCCGACT
    ACAGTGGGTA CTGTGAGCAG TACTGATGCT ACTGTCACTG CTGCCACCAC TGAAGCCACA
    ACAGTCCCCA TCATCCCAAC TGTCGCACCT GCCACCATCG CTACCACCAC CGTCGCCACA
    ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACGG AGGGTCCTCC CACCACCACC
    ACCGGGACTA AGATACACGA ATCCGCCCCG GACGAGCAGT CCATATGGAA CGTCACAGTG
    CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA GTGTCCCTGT TAAGGCCCAG
    GCGCAGCCTG TCCAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGAGTCTAT
    TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC
    ACCAACAACC AGGCGGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGCGCCATC
    GCCCTCCTGG TGCTGATTCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG AGGCGGCAAG
    TACCCAGTGC GAGAGAAGAA GGACGTTCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAC
    GGCTCCTTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCCCTG
    GATGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTGG TGGACTACGG CGAGGGCGGC
    GAGGGGCAGT TCAATGAGGA CGGCTCCTTT ATCGGCCAGT ACACAGTCAA AAAGGACAAG
    GAGGAGACAG AGGGCAATGA AAGCTCAGAG GCCACGTCAC CTGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014595001.1
    CDS196..3864
    Translation

    Target ORF information:

    RefSeq Version XM_014739515.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739515.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTTACCC AGTGCCCCCA GGCGATTCCG AGTCCGGCAG
    CCCAACCTGG AGACCATCAA CCTGGAATGG GACCATCCGG AACACCCCAA TGGGATCCTG
    ACTGGATACA CCCTCAGATA CGTGCCCTTT AATGGAACCA AAGTAGGAAA GCAGATAGTG
    GAAAACTTCT CTCCCAATCA GACCAAGTTC ACGGTGCAAA GAGCAGACCC CGTGTCGCGC
    TACCGCTTTA CCCTCAGTGC CAGGACGCAG GTGGGCTCTG GGGAAGCTGT CACAGAGGAG
    TCGCCAGCAC CCCCAAATGA AGCTACTCCA ACCGCAGCTC CTCCCACGTT GCCCCCGACT
    ACAGTGGGTA CTGTGAGCAG TACTGATGCT ACTGTCACTG CTGCCACCAC TGAAGCCACA
    ACAGTCCCCA TCATCCCAAC TGTCGCACCT GCCACCATCG CTACCACCAC CGTCGCCACA
    ACTACTACAA CCACTGCCGC CACCACCACC ACCACCACGG AGGGTCCTCC CACCACCACC
    ACCGGGACTA AGATACACGA ATCCGCCCCG GACGAGCAGT CCATATGGAA CGTCACAGTG
    CTCCCCAACA GTAAATGGGC CAACATCACC TGGAAGCACA ATTTCGGGCC CGGAACTGAC
    TTTGTGGTTG AGTACATCGA CAGCAACCAT ACGAAAAAAA GTGTCCCTGT TAAGGCCCAG
    GCGCAGCCTG TCCAGCTGAC AGACCTCTAT CCCGGGATGA CATACACGTT GCGAGTCTAT
    TCCCGGGACA ACGAGGGCAT CAGCAGTACC GTCATCACCT TTATGACCAG TACAGCTTAC
    ACCAACAACC AGGCGGACAT CGCCACCCAG GGCTGGTTCA TCGGGCTCAT GTGCGCCATC
    GCCCTCCTGG TGCTGATTCT GCTCATCGTC TGTTTCATCA AGAGGAGTCG AGGCGGCAAG
    TACCCAGTGC GAGAGAAGAA GGACGTTCCC CTCGGCCCTG AAGACCCCAA AGAAGAGGAC
    GGCTCCTTTG ACTACAGTGA TGAGGACAAC AAGCCCCTGC AGGGCAGCCA GACATCCCTG
    GATGGCACCA TCAAGCAGCA GGAGAGTGAC GACAGCCTGG TGGACTACGG CGAGGGCGGC
    GAGGGGCAGT TCAATGAGGA CGGCTCCTTT ATCGGCCAGT ACACAGTCAA AAAGGACAAG
    GAGGAGACAG AGGGCAATGA AAGCTCAGAG GCCACGTCAC CTGTCAACGC CATCTATTCT
    CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30469
    Clone ID Related Accession (Same CDS sequence) XM_014739502.1 , XM_014739501.1
    Accession Version XM_014739502.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594988.1
    CDS196..4236
    Translation

    Target ORF information:

    RefSeq Version XM_014739502.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739502.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30469
    Clone ID Related Accession (Same CDS sequence) XM_014739502.1 , XM_014739501.1
    Accession Version XM_014739501.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4041bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594987.1
    CDS196..4236
    Translation

    Target ORF information:

    RefSeq Version XM_014739501.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739501.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTATCC CAGGGCTGCG CCCACCGACG TTAAAATCCG AGTGCTGAAC
    AGCACGGCCA TCAGCCTTCA GTGGAACCGC GTCTACTCCG ACACGGTCCA GGGCCTGCTC
    AGAGAGTACC GAGCCTACTA CTGGAGAGAG AGCAGCTTGC TGAAGAGCTT GTGGGTGTCT
    CAGAAGAGAC AGCAGGCCAG CTTCCCTGGT GACCGCCTCC GCGGCGTGGT GCCCCGCCTC
    TTCCCCTACA GCAACTACAA GCTGGAGATG GTTGTGGTCA ATGGGAGAGG CGATGGGCCT
    CGCAGTGAGA CCAAGGAGTT CACAACCCCA GAAGGAGTAC CCAGTGCCCC CAGGCGATTC
    CGAGTCCGGC AGCCCAACCT GGAGACCATC AACCTGGAAT GGGACCATCC GGAACACCCC
    AATGGGATCC TGACTGGATA CACCCTCAGA TACGTGCCCT TTAATGGAAC CAAAGTAGGA
    AAGCAGATAG TGGAAAACTT CTCTCCCAAT CAGACCAAGT TCACGGTGCA AAGAGCAGAC
    CCCGTGTCGC GCTACCGCTT TACCCTCAGT GCCAGGACGC AGGTGGGCTC TGGGGAAGCT
    GTCACAGAGG AGTCGCCAGC ACCCCCAAAT GAAGCTACTC CAACCGCAGC TCCTCCCACG
    TTGCCCCCGA CTACAGTGGG TACTGTGAGC AGTACTGATG CTACTGTCAC TGCTGCCACC
    ACTGAAGCCA CAACAGTCCC CATCATCCCA ACTGTCGCAC CTGCCACCAT CGCTACCACC
    ACCGTCGCCA CAACTACTAC AACCACTGCC GCCACCACCA CCACCACCAC GGAGGGTCCT
    CCCACCACCA CCACCGGGAC TAAGATACAC GAATCCGCCC CGGACGAGCA GTCCATATGG
    AACGTCACAG TGCTCCCCAA CAGTAAATGG GCCAACATCA CCTGGAAGCA CAATTTCGGG
    CCCGGAACTG ACTTTGTGGT TGAGTACATC GACAGCAACC ATACGAAAAA AAGTGTCCCT
    GTTAAGGCCC AGGCGCAGCC TGTCCAGCTG ACAGACCTCT ATCCCGGGAT GACATACACG
    TTGCGAGTCT ATTCCCGGGA CAACGAGGGC ATCAGCAGTA CCGTCATCAC CTTTATGACC
    AGTACAGCTT ACACCAACAA CCAGGCGGAC ATCGCCACCC AGGGCTGGTT CATCGGGCTC
    ATGTGCGCCA TCGCCCTCCT GGTGCTGATT CTGCTCATCG TCTGTTTCAT CAAGAGGAGT
    CGAGGCGGCA AGTACCCAGT GCGAGAGAAG AAGGACGTTC CCCTCGGCCC TGAAGACCCC
    AAAGAAGAGG ACGGCTCCTT TGACTACAGT GATGAGGACA ACAAGCCCCT GCAGGGCAGC
    CAGACATCCC TGGATGGCAC CATCAAGCAG CAGGAGAGTG ACGACAGCCT GGTGGACTAC
    GGCGAGGGCG GCGAGGGGCA GTTCAATGAG GACGGCTCCT TTATCGGCCA GTACACAGTC
    AAAAAGGACA AGGAGGAGAC AGAGGGCAAT GAAAGCTCAG AGGCCACGTC ACCTGTCAAC
    GCCATCTATT CTCTGGCCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30470
    Clone ID Related Accession (Same CDS sequence) XM_014739504.1
    Accession Version XM_014739504.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACCGACGT TAAAATCCGA
    GTGCTGAACA GCACGGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCTGCTCA GAGAGTACCG AGCCTACTAC TGGAGAGAGA GCAGCTTGCT GAAGAGCTTG
    TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGCCTCCG CGGCGTGGTG
    CCCCGCCTCT TCCCCTACAG CAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGC
    GATGGGCCTC GCAGTGAGAC CAAGGAGTTC ACAACCCCAG AAGGAGTACC CAGTGCCCCC
    AGGCGATTCC GAGTCCGGCA GCCCAACCTG GAGACCATCA ACCTGGAATG GGACCATCCG
    GAACACCCCA ATGGGATCCT GACTGGATAC ACCCTCAGAT ACGTGCCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT ACCCTCAGTG CCAGGACGCA GGTGGGCTCT
    GGGGAAGCTG TCACAGAGGA GTCGCCAGCA CCCCCAAATG AAGCTACTCC AACCGCAGCT
    CCTCCCACGT TGCCCCCGAC TACAGTGGGT ACTGTGAGCA GTACTGATGC TACTGTCACT
    GCTGCCACCA CTGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TGCCACCATC
    GCTACCACCA CCGTCGCCAC AACTACTACA ACCACTGCCG CCACCACCAC CACCACCACG
    GAGGGTCCTC CCACCACCAC CACCGGGACT AAGATACACG AATCCGCCCC GGACGAGCAG
    TCCATATGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT GTCCAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGAGTCTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG GTGCTGATTC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAGAAGA AGGACGTTCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA CGGCTCCTTT GACTACAGTG ATGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCCCT GGATGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG
    GTGGACTACG GCGAGGGCGG CGAGGGGCAG TTCAATGAGG ACGGCTCCTT TATCGGCCAG
    TACACAGTCA AAAAGGACAA GGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACGTCA
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594990.1
    CDS196..4185
    Translation

    Target ORF information:

    RefSeq Version XM_014739504.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739504.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCACCCG AGGAGTTGCG
    GAAAGGACAC CCAGCTTCAT GTATCCCCAG GGCACTGCGA GCAGCCAGAT GGTGCTGCGT
    GGCATGGACC TCCTGCTGGA GTGCATCGCC TCCGGGGTCC CGACACCAGA CATCGCGTGG
    TACAAGAAAG GTGGGGACCT CCCATCTGAC AAGGCCAAGT TTGAGAACTT TAACAAGGCT
    CTACGTATCA CCAATGTCTC TGAGGAAGAC TCTGGAGAGT ATTTCTGCCT GGCCTCCAAC
    AAGATGGGCA GCATCCGGCA CACGATCTCG GTGAGAGTAA AGGCTGCTCC CTACTGGCTG
    GATGAACCCA AGAACCTTAT CCTGGCTCCT GGCGAGGACG GGAGACTGGT GTGTCGAGCC
    AGTGGGAACC CCAAGCCCAC CATCCAGTGG ATGGTGAATG GGGAACCTTT GCAATCGGCA
    CCACCCAACC CAAACCGAGA GGTGGCCGGG GACACCATCA TCTTCCGGGA CACGCAAATC
    AGCAGCAGGG CCGTGTACCA GTGCAACACG TCCAACGAGC ACGGCTACCT GCTGGCCAAC
    GCCTTCGTCA GCGTGCTGGA CGTGCCACCT CGGATGCTGT CACCGCGGAA CCAGCTTATT
    AGGGTGATCC TGTACAACCG GACGCGGCTG GACTGCCCAT TCTTTGGGTC CCCCATTCCC
    ACGCTCCGTT GGTTTAAGAA TGGACAAGGA AGCAACCTGG ATGGAGGCAA CTACCATGTC
    TATGAGAATG GCAGTCTGGA AATCAAGATG ATCCACAAAG AGGACCAAGG CATCTACACC
    TGCGTTGCCA CTAACATCCT GGGCAAGGCA GAAAACCAGG TCCGCCTGGA AGTCAAAGAC
    CCCACCAGGA TCTTTCGGAT GCCTGAGGAC CAGGTTGCCA AGAGGGGCAC CACGGTGCAG
    CTGGAGTGCC GGGTGAAGCA CGACCCCTCC CTGAAGCTCA CAGTGTCCTG GCTGAAGGAT
    GACGAGCCGC TCTACATCGG GAACAGGATG AAGAAGGAAG ATGACTCCCT GACCATCTTT
    GGTGTGGCGG AGCGGGACCA GGGCAGTTAC ACCTGCGTCG CCAGCACTGA GCTGGACCAA
    GACCTGGCCA AGGCCTACCT CACCGTGCTA GGACGGCCAG ACAGACCCCG GGACCTGGAG
    CTCACTGACC TGGCTGAGAG GAGTGTGAGG CTCACCTGGA TCCCAGGGGA TGACAACAAT
    AGCCCCATCA CAGACTATGT CGTCCAGTTT GAAGAGGACC AGTTCCAGCC AGGGGTCTGG
    CATGACCATT CCAAGTTCCC AGGCAGTGTC AACTCAGCTG TCCTCCAGCT GTCCCCATAT
    GTCAACTACC AGTTCCGGGT CATTGCCATC AACGAGGTCG GCAGCAGCCA CCCCAGCCTC
    CCATCTGAGC GCTACCGAAC CAGCGGAGCA CCCCCTGAGT CTAATCCCGC TGATGTGAAG
    GGAGAGGGGA CCAGAAAAAA CAACATGGAG ATCACGTGGA CGCCCATGAA TGCCACCTCC
    GCCTTTGGCC CCAACCTGCG CTACATCGTG AAGTGGCGGC GGAGAGAGAC TCGAGAGAGC
    TGGAACAACG TCACAGTGTG GGGCTCCCGC TACGTGGTGG GTCAGACCCC TGTCTATGTG
    CCCTACGAGA TCCGAGTCCA GGCCGAAAAT GACTTTGGGA AGGGCCCTGA GCCAGACACG
    GTCATCGGTT ACTCCGGAGA AGATTATCCC AGGGCTGCGC CCACCGACGT TAAAATCCGA
    GTGCTGAACA GCACGGCCAT CAGCCTTCAG TGGAACCGCG TCTACTCCGA CACGGTCCAG
    GGCCTGCTCA GAGAGTACCG AGCCTACTAC TGGAGAGAGA GCAGCTTGCT GAAGAGCTTG
    TGGGTGTCTC AGAAGAGACA GCAGGCCAGC TTCCCTGGTG ACCGCCTCCG CGGCGTGGTG
    CCCCGCCTCT TCCCCTACAG CAACTACAAG CTGGAGATGG TTGTGGTCAA TGGGAGAGGC
    GATGGGCCTC GCAGTGAGAC CAAGGAGTTC ACAACCCCAG AAGGAGTACC CAGTGCCCCC
    AGGCGATTCC GAGTCCGGCA GCCCAACCTG GAGACCATCA ACCTGGAATG GGACCATCCG
    GAACACCCCA ATGGGATCCT GACTGGATAC ACCCTCAGAT ACGTGCCCTT TAATGGAACC
    AAAGTAGGAA AGCAGATAGT GGAAAACTTC TCTCCCAATC AGACCAAGTT CACGGTGCAA
    AGAGCAGACC CCGTGTCGCG CTACCGCTTT ACCCTCAGTG CCAGGACGCA GGTGGGCTCT
    GGGGAAGCTG TCACAGAGGA GTCGCCAGCA CCCCCAAATG AAGCTACTCC AACCGCAGCT
    CCTCCCACGT TGCCCCCGAC TACAGTGGGT ACTGTGAGCA GTACTGATGC TACTGTCACT
    GCTGCCACCA CTGAAGCCAC AACAGTCCCC ATCATCCCAA CTGTCGCACC TGCCACCATC
    GCTACCACCA CCGTCGCCAC AACTACTACA ACCACTGCCG CCACCACCAC CACCACCACG
    GAGGGTCCTC CCACCACCAC CACCGGGACT AAGATACACG AATCCGCCCC GGACGAGCAG
    TCCATATGGA ACGTCACAGT GCTCCCCAAC AGTAAATGGG CCAACATCAC CTGGAAGCAC
    AATTTCGGGC CCGGAACTGA CTTTGTGGTT GAGTACATCG ACAGCAACCA TACGAAAAAA
    AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT GTCCAGCTGA CAGACCTCTA TCCCGGGATG
    ACATACACGT TGCGAGTCTA TTCCCGGGAC AACGAGGGCA TCAGCAGTAC CGTCATCACC
    TTTATGACCA GTACAGCTTA CACCAACAAC CAGGCGGACA TCGCCACCCA GGGCTGGTTC
    ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG GTGCTGATTC TGCTCATCGT CTGTTTCATC
    AAGAGGAGTC GAGGCGGCAA GTACCCAGTG CGAGAGAAGA AGGACGTTCC CCTCGGCCCT
    GAAGACCCCA AAGAAGAGGA CGGCTCCTTT GACTACAGTG ATGAGGACAA CAAGCCCCTG
    CAGGGCAGCC AGACATCCCT GGATGGCACC ATCAAGCAGC AGGAGAGTGA CGACAGCCTG
    GTGGACTACG GCGAGGGCGG CGAGGGGCAG TTCAATGAGG ACGGCTCCTT TATCGGCCAG
    TACACAGTCA AAAAGGACAA GGAGGAGACA GAGGGCAATG AAAGCTCAGA GGCCACGTCA
    CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30471
    Clone ID Related Accession (Same CDS sequence) XM_014739505.1
    Accession Version XM_014739505.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CGGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACTGC GAGCAGCCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG
    GAGTGCATCG CCTCCGGGGT CCCGACACCA GACATCGCGT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTACGTAT CACCAATGTC
    TCTGAGGAAG ACTCTGGAGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGATGAACC CAAGAACCTT
    ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAGTGGGAA CCCCAAGCCC
    ACCATCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGA
    GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACGCAAA TCAGCAGCAG GGCCGTGTAC
    CAGTGCAACA CGTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CTCGGATGCT GTCACCGCGG AACCAGCTTA TTAGGGTGAT CCTGTACAAC
    CGGACGCGGC TGGACTGCCC ATTCTTTGGG TCCCCCATTC CCACGCTCCG TTGGTTTAAG
    AATGGACAAG GAAGCAACCT GGATGGAGGC AACTACCATG TCTATGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCACAA AGAGGACCAA GGCATCTACA CCTGCGTTGC CACTAACATC
    CTGGGCAAGG CAGAAAACCA GGTCCGCCTG GAAGTCAAAG ACCCCACCAG GATCTTTCGG
    ATGCCTGAGG ACCAGGTTGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACAGTGTCC TGGCTGAAGG ATGACGAGCC GCTCTACATC
    GGGAACAGGA TGAAGAAGGA AGATGACTCC CTGACCATCT TTGGTGTGGC GGAGCGGGAC
    CAGGGCAGTT ACACCTGCGT CGCCAGCACT GAGCTGGACC AAGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCAAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594991.1
    CDS196..4167
    Translation

    Target ORF information:

    RefSeq Version XM_014739505.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739505.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CTGTCCCAGC CTCCGACCAT CACCAAGCAG
    TCGGTGAAGG ACCACATCGT GGATCCCCGG GATAACATCC TGATTGAGTG TGAGGCGAAG
    GGGAACCCCG CCCCCAGCTT CCACTGGACT CGCAACAGCA GGTTCTTCAA CATCGCCAAG
    GACCCCCGGG TGTCCATGAA GAGGCGGTCT GGGACCCTGG TGATCGACTT CCGCAGCGGC
    GGGCGGCCAG AGGAGTACGA GGGGGAATAC CAGTGCTTCG CCCGCAACAA GTTCGGCACC
    GCCCTGTCCA ACAGGATCCG CCTGCAGGTG TCCAAATCTC CCCTGTGGCC CAAGGAAAAC
    CTAGACCCTG TCGTGGTCCA AGAAGGCGCC CCCTTGACAC TCCAGTGTAA CCCCCCGCCT
    GGGCTTCCGT CCCCAGTCAT CTTCTGGATG AGCAGCACCA TGGACCCCAT CACCCAAGAC
    AAGCGTGTCT CCCAGGGCCA CAACGGAGAC CTGTATTTCT CCAACGTCAT GCTGCAGGAC
    ATGCAGACTG ACTACAGCTG CAATGCCCGC TTCCACTTTA CCCACACCAT CCAGCAGAAG
    AACGCGTTCA CCCTCAAGGT CCTCACCACC CGAGGAGTTG CGGAAAGGAC ACCCAGCTTC
    ATGTATCCCC AGGGCACTGC GAGCAGCCAG ATGGTGCTGC GTGGCATGGA CCTCCTGCTG
    GAGTGCATCG CCTCCGGGGT CCCGACACCA GACATCGCGT GGTACAAGAA AGGTGGGGAC
    CTCCCATCTG ACAAGGCCAA GTTTGAGAAC TTTAACAAGG CTCTACGTAT CACCAATGTC
    TCTGAGGAAG ACTCTGGAGA GTATTTCTGC CTGGCCTCCA ACAAGATGGG CAGCATCCGG
    CACACGATCT CGGTGAGAGT AAAGGCTGCT CCCTACTGGC TGGATGAACC CAAGAACCTT
    ATCCTGGCTC CTGGCGAGGA CGGGAGACTG GTGTGTCGAG CCAGTGGGAA CCCCAAGCCC
    ACCATCCAGT GGATGGTGAA TGGGGAACCT TTGCAATCGG CACCACCCAA CCCAAACCGA
    GAGGTGGCCG GGGACACCAT CATCTTCCGG GACACGCAAA TCAGCAGCAG GGCCGTGTAC
    CAGTGCAACA CGTCCAACGA GCACGGCTAC CTGCTGGCCA ACGCCTTCGT CAGCGTGCTG
    GACGTGCCAC CTCGGATGCT GTCACCGCGG AACCAGCTTA TTAGGGTGAT CCTGTACAAC
    CGGACGCGGC TGGACTGCCC ATTCTTTGGG TCCCCCATTC CCACGCTCCG TTGGTTTAAG
    AATGGACAAG GAAGCAACCT GGATGGAGGC AACTACCATG TCTATGAGAA TGGCAGTCTG
    GAAATCAAGA TGATCCACAA AGAGGACCAA GGCATCTACA CCTGCGTTGC CACTAACATC
    CTGGGCAAGG CAGAAAACCA GGTCCGCCTG GAAGTCAAAG ACCCCACCAG GATCTTTCGG
    ATGCCTGAGG ACCAGGTTGC CAAGAGGGGC ACCACGGTGC AGCTGGAGTG CCGGGTGAAG
    CACGACCCCT CCCTGAAGCT CACAGTGTCC TGGCTGAAGG ATGACGAGCC GCTCTACATC
    GGGAACAGGA TGAAGAAGGA AGATGACTCC CTGACCATCT TTGGTGTGGC GGAGCGGGAC
    CAGGGCAGTT ACACCTGCGT CGCCAGCACT GAGCTGGACC AAGACCTGGC CAAGGCCTAC
    CTCACCGTGC TAGGACGGCC AGACAGACCC CGGGACCTGG AGCTCACTGA CCTGGCTGAG
    AGGAGTGTGA GGCTCACCTG GATCCCAGGG GATGACAACA ATAGCCCCAT CACAGACTAT
    GTCGTCCAGT TTGAAGAGGA CCAGTTCCAG CCAGGGGTCT GGCATGACCA TTCCAAGTTC
    CCAGGCAGTG TCAACTCAGC TGTCCTCCAG CTGTCCCCAT ATGTCAACTA CCAGTTCCGG
    GTCATTGCCA TCAACGAGGT CGGCAGCAGC CACCCCAGCC TCCCATCTGA GCGCTACCGA
    ACCAGCGGAG CACCCCCTGA GTCTAATCCC GCTGATGTGA AGGGAGAGGG GACCAGAAAA
    AACAACATGG AGATCACGTG GACGCCCATG AATGCCACCT CCGCCTTTGG CCCCAACCTG
    CGCTACATCG TGAAGTGGCG GCGGAGAGAG ACTCGAGAGA GCTGGAACAA CGTCACAGTG
    TGGGGCTCCC GCTACGTGGT GGGTCAGACC CCTGTCTATG TGCCCTACGA GATCCGAGTC
    CAGGCCGAAA ATGACTTTGG GAAGGGCCCT GAGCCAGACA CGGTCATCGG TTACTCCGGA
    GAAGATTATC CCAGGGCTGC GCCCACCGAC GTTAAAATCC GAGTGCTGAA CAGCACGGCC
    ATCAGCCTTC AGTGGAACCG CGTCTACTCC GACACGGTCC AGGGCCTGCT CAGAGAGTAC
    CGAGCCTACT ACTGGAGAGA GAGCAGCTTG CTGAAGAGCT TGTGGGTGTC TCAGAAGAGA
    CAGCAGGCCA GCTTCCCTGG TGACCGCCTC CGCGGCGTGG TGCCCCGCCT CTTCCCCTAC
    AGCAACTACA AGCTGGAGAT GGTTGTGGTC AATGGGAGAG GCGATGGGCC TCGCAGTGAG
    ACCAAGGAGT TCACAACCCC AGAAGGAGTA CCCAGTGCCC CCAGGCGATT CCGAGTCCGG
    CAGCCCAACC TGGAGACCAT CAACCTGGAA TGGGACCATC CGGAACACCC CAATGGGATC
    CTGACTGGAT ACACCCTCAG ATACGTGCCC TTTAATGGAA CCAAAGTAGG AAAGCAGATA
    GTGGAAAACT TCTCTCCCAA TCAGACCAAG TTCACGGTGC AAAGAGCAGA CCCCGTGTCG
    CGCTACCGCT TTACCCTCAG TGCCAGGACG CAGGTGGGCT CTGGGGAAGC TGTCACAGAG
    GAGTCGCCAG CACCCCCAAA TGAAGCTACT CCAACCGCAG CTCCTCCCAC GTTGCCCCCG
    ACTACAGTGG GTACTGTGAG CAGTACTGAT GCTACTGTCA CTGCTGCCAC CACTGAAGCC
    ACAACAGTCC CCATCATCCC AACTGTCGCA CCTGCCACCA TCGCTACCAC CACCGTCGCC
    ACAACTACTA CAACCACTGC CGCCACCACC ACCACCACCA CGGAGGGTCC TCCCACCACC
    ACCACCGGGA CTAAGATACA CGAATCCGCC CCGGACGAGC AGTCCATATG GAACGTCACA
    GTGCTCCCCA ACAGTAAATG GGCCAACATC ACCTGGAAGC ACAATTTCGG GCCCGGAACT
    GACTTTGTGG TTGAGTACAT CGACAGCAAC CATACGAAAA AAAGTGTCCC TGTTAAGGCC
    CAGGCGCAGC CTGTCCAGCT GACAGACCTC TATCCCGGGA TGACATACAC GTTGCGAGTC
    TATTCCCGGG ACAACGAGGG CATCAGCAGT ACCGTCATCA CCTTTATGAC CAGTACAGCT
    TACACCAACA ACCAGGCGGA CATCGCCACC CAGGGCTGGT TCATCGGGCT CATGTGCGCC
    ATCGCCCTCC TGGTGCTGAT TCTGCTCATC GTCTGTTTCA TCAAGAGGAG TCGAGGCGGC
    AAGTACCCAG TGCGAGAGAA GAAGGACGTT CCCCTCGGCC CTGAAGACCC CAAAGAAGAG
    GACGGCTCCT TTGACTACAG TGATGAGGAC AACAAGCCCC TGCAGGGCAG CCAGACATCC
    CTGGATGGCA CCATCAAGCA GCAGGAGAGT GACGACAGCC TGGTGGACTA CGGCGAGGGC
    GGCGAGGGGC AGTTCAATGA GGACGGCTCC TTTATCGGCC AGTACACAGT CAAAAAGGAC
    AAGGAGGAGA CAGAGGGCAA TGAAAGCTCA GAGGCCACGT CACCTGTCAA CGCCATCTAT
    TCTCTGGCCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb30472
    Clone ID Related Accession (Same CDS sequence) XM_014739506.1
    Accession Version XM_014739506.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3720bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product neurofascin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867416.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTACTCC AACCGCAGCT CCTCCCACGT TGCCCCCGAC TACAGTGGGT
    ACTGTGAGCA GTACTGATGC TACTGTCACT GCTGCCACCA CTGAAGCCAC AACAGTCCCC
    ATCATCCCAA CTGTCGCACC TGCCACCATC GCTACCACCA CCGTCGCCAC AACTACTACA
    ACCACTGCCG CCACCACCAC CACCACCACG GAGGGTCCTC CCACCACCAC CACCGGGACT
    AAGATACACG AATCCGCCCC GGACGAGCAG TCCATATGGA ACGTCACAGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACATCG ACAGCAACCA TACGAAAAAA AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT
    GTCCAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGAGTCTA TTCCCGGGAC
    AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_014594992.1
    CDS196..3915
    Translation

    Target ORF information:

    RefSeq Version XM_014739506.1
    Organism Equus caballus(horse)
    Definition Equus caballus neurofascin (NFASC), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014739506.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCCAGGC AGCAGCTGCC ACCCTGGGTC CATGCAGCCA TCCTCCTCTG CCTCCTCAGC 
    CTCGGCCGGG CCATCGAGAT TCCTATGGAT CCAAGCATTC AGAACGAGCT GTCCCAGCCT
    CCGACCATCA CCAAGCAGTC GGTGAAGGAC CACATCGTGG ATCCCCGGGA TAACATCCTG
    ATTGAGTGTG AGGCGAAGGG GAACCCCGCC CCCAGCTTCC ACTGGACTCG CAACAGCAGG
    TTCTTCAACA TCGCCAAGGA CCCCCGGGTG TCCATGAAGA GGCGGTCTGG GACCCTGGTG
    ATCGACTTCC GCAGCGGCGG GCGGCCAGAG GAGTACGAGG GGGAATACCA GTGCTTCGCC
    CGCAACAAGT TCGGCACCGC CCTGTCCAAC AGGATCCGCC TGCAGGTGTC CAAATCTCCC
    CTGTGGCCCA AGGAAAACCT AGACCCTGTC GTGGTCCAAG AAGGCGCCCC CTTGACACTC
    CAGTGTAACC CCCCGCCTGG GCTTCCGTCC CCAGTCATCT TCTGGATGAG CAGCACCATG
    GACCCCATCA CCCAAGACAA GCGTGTCTCC CAGGGCCACA ACGGAGACCT GTATTTCTCC
    AACGTCATGC TGCAGGACAT GCAGACTGAC TACAGCTGCA ATGCCCGCTT CCACTTTACC
    CACACCATCC AGCAGAAGAA CGCGTTCACC CTCAAGGTCC TCACCAACCA CCCCCATAAT
    GACTCGTCCT TAAGAAACCA CCCTGACATG TACAGTGCCC GAGGAGTTGC GGAAAGGACA
    CCCAGCTTCA TGTATCCCCA GGGCACTGCG AGCAGCCAGA TGGTGCTGCG TGGCATGGAC
    CTCCTGCTGG AGTGCATCGC CTCCGGGGTC CCGACACCAG ACATCGCGTG GTACAAGAAA
    GGTGGGGACC TCCCATCTGA CAAGGCCAAG TTTGAGAACT TTAACAAGGC TCTACGTATC
    ACCAATGTCT CTGAGGAAGA CTCTGGAGAG TATTTCTGCC TGGCCTCCAA CAAGATGGGC
    AGCATCCGGC ACACGATCTC GGTGAGAGTA AAGGCTGCTC CCTACTGGCT GGATGAACCC
    AAGAACCTTA TCCTGGCTCC TGGCGAGGAC GGGAGACTGG TGTGTCGAGC CAGTGGGAAC
    CCCAAGCCCA CCATCCAGTG GATGGTGAAT GGGGAACCTT TGCAATCGGC ACCACCCAAC
    CCAAACCGAG AGGTGGCCGG GGACACCATC ATCTTCCGGG ACACGCAAAT CAGCAGCAGG
    GCCGTGTACC AGTGCAACAC GTCCAACGAG CACGGCTACC TGCTGGCCAA CGCCTTCGTC
    AGCGTGCTGG ACGTGCCACC TCGGATGCTG TCACCGCGGA ACCAGCTTAT TAGGGTGATC
    CTGTACAACC GGACGCGGCT GGACTGCCCA TTCTTTGGGT CCCCCATTCC CACGCTCCGT
    TGGTTTAAGA ATGGACAAGG AAGCAACCTG GATGGAGGCA ACTACCATGT CTATGAGAAT
    GGCAGTCTGG AAATCAAGAT GATCCACAAA GAGGACCAAG GCATCTACAC CTGCGTTGCC
    ACTAACATCC TGGGCAAGGC AGAAAACCAG GTCCGCCTGG AAGTCAAAGA CCCCACCAGG
    ATCTTTCGGA TGCCTGAGGA CCAGGTTGCC AAGAGGGGCA CCACGGTGCA GCTGGAGTGC
    CGGGTGAAGC ACGACCCCTC CCTGAAGCTC ACAGTGTCCT GGCTGAAGGA TGACGAGCCG
    CTCTACATCG GGAACAGGAT GAAGAAGGAA GATGACTCCC TGACCATCTT TGGTGTGGCG
    GAGCGGGACC AGGGCAGTTA CACCTGCGTC GCCAGCACTG AGCTGGACCA AGACCTGGCC
    AAGGCCTACC TCACCGTGCT AGGACGGCCA GACAGACCCC GGGACCTGGA GCTCACTGAC
    CTGGCTGAGA GGAGTGTGAG GCTCACCTGG ATCCCAGGGG ATGACAACAA TAGCCCCATC
    ACAGACTATG TCGTCCAGTT TGAAGAGGAC CAGTTCCAGC CAGGGGTCTG GCATGACCAT
    TCCAAGTTCC CAGGCAGTGT CAACTCAGCT GTCCTCCAGC TGTCCCCATA TGTCAACTAC
    CAGTTCCGGG TCATTGCCAT CAACGAGGTC GGCAGCAGCC ACCCCAGCCT CCCATCTGAG
    CGCTACCGAA CCAGCGGAGC ACCCCCTGAG TCTAATCCCG CTGATGTGAA GGGAGAGGGG
    ACCAGAAAAA ACAACATGGA GATCACGTGG ACGCCCATGA ATGCCACCTC CGCCTTTGGC
    CCCAACCTGC GCTACATCGT GAAGTGGCGG CGGAGAGAGA CTCGAGAGAG CTGGAACAAC
    GTCACAGTGT GGGGCTCCCG CTACGTGGTG GGTCAGACCC CTGTCTATGT GCCCTACGAG
    ATCCGAGTCC AGGCCGAAAA TGACTTTGGG AAGGGCCCTG AGCCAGACAC GGTCATCGGT
    TACTCCGGAG AAGATTTACC CAGTGCCCCC AGGCGATTCC GAGTCCGGCA GCCCAACCTG
    GAGACCATCA ACCTGGAATG GGACCATCCG GAACACCCCA ATGGGATCCT GACTGGATAC
    ACCCTCAGAT ACGTGCCCTT TAATGGAACC AAAGTAGGAA AGCAGATAGT GGAAAACTTC
    TCTCCCAATC AGACCAAGTT CACGGTGCAA AGAGCAGACC CCGTGTCGCG CTACCGCTTT
    ACCCTCAGTG CCAGGACGCA GGTGGGCTCT GGGGAAGCTG TCACAGAGGA GTCGCCAGCA
    CCCCCAAATG AAGCTACTCC AACCGCAGCT CCTCCCACGT TGCCCCCGAC TACAGTGGGT
    ACTGTGAGCA GTACTGATGC TACTGTCACT GCTGCCACCA CTGAAGCCAC AACAGTCCCC
    ATCATCCCAA CTGTCGCACC TGCCACCATC GCTACCACCA CCGTCGCCAC AACTACTACA
    ACCACTGCCG CCACCACCAC CACCACCACG GAGGGTCCTC CCACCACCAC CACCGGGACT
    AAGATACACG AATCCGCCCC GGACGAGCAG TCCATATGGA ACGTCACAGT GCTCCCCAAC
    AGTAAATGGG CCAACATCAC CTGGAAGCAC AATTTCGGGC CCGGAACTGA CTTTGTGGTT
    GAGTACATCG ACAGCAACCA TACGAAAAAA AGTGTCCCTG TTAAGGCCCA GGCGCAGCCT
    GTCCAGCTGA CAGACCTCTA TCCCGGGATG ACATACACGT TGCGAGTCTA TTCCCGGGAC
    AACGAGGGCA TCAGCAGTAC CGTCATCACC TTTATGACCA GTACAGCTTA CACCAACAAC
    CAGGCGGACA TCGCCACCCA GGGCTGGTTC ATCGGGCTCA TGTGCGCCAT CGCCCTCCTG
    GTGCTGATTC TGCTCATCGT CTGTTTCATC AAGAGGAGTC GAGGCGGCAA GTACCCAGTG
    CGAGAGAAGA AGGACGTTCC CCTCGGCCCT GAAGACCCCA AAGAAGAGGA CGGCTCCTTT
    GACTACAGTG ATGAGGACAA CAAGCCCCTG CAGGGCAGCC AGACATCCCT GGATGGCACC
    ATCAAGCAGC AGGAGAGTGA CGACAGCCTG GTGGACTACG GCGAGGGCGG CGAGGGGCAG
    TTCAATGAGG ACGGCTCCTT TATCGGCCAG TACACAGTCA AAAAGGACAA GGAGGAGACA
    GAGGGCAATG AAAGCTCAGA GGCCACGTCA CCTGTCAACG CCATCTATTC TCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.