POLR2B cDNA ORF clone, Equus caballus(horse)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLR2B
Entrez Gene ID	100053708
Full Name	RNA polymerase II subunit B
General protein information	Preferred Names RNA polymerase II subunit B Names LOW QUALITY PROTEIN: DNA-directed RNA polymerase II subunit RPB2 polymerase (RNA) II (DNA directed) polypeptide B, 140kDa
Gene Type	protein-coding
Organism	Equus caballus(horse)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001916791.5	XP_001916826.2	DNA-directed RNA polymerase II subunit RPB2
XM_001916791.4	XP_001916826.1	LOW QUALITY PROTEIN: DNA-directed RNA polymerase II subunit RPB2

Pathways from BioSystems
Gene Ontology

The following POLR2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLR2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHb62538	XM_001916791.5 Latest version!	Equus caballus RNA polymerase II subunit B (POLR2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHb18922	XM_001916791.4 Latest version!	Equus caballus polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLR2B ( XM_001916791.5 ) cDNA ORF clone, Equus caballus(horse) -> XP_001916826.2 Equus caballus RNA polymerase II subunit B (POLR2B), mRNA.

Price & Availability↑

CloneID	OHb62538
Clone ID Related Accession (Same CDS sequence)	XM_001916791.5
Accession Version	XM_001916791.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-01-22
Organism	Equus caballus(horse)
Product	DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009146.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001916791.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGACG CGGACGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCAGAT 
TTGTGGCAAG AAGCTTGCTG GATTGTAATC AGTTCTTATT TTGACGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCTCCAC CTATAGACCT GCAAGCTGAA GCTCAGCATG CCAGTGGGGA AGTTGAAGAA 
CCGCCAAGAT ATTTGCTGAA GTTTGAACAA ATTTACCTCT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCA AATGAGGCCA GATTAAGAAA TCTCACGTAT 
TCTGCTCCAC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAGAC TTTTATAGGA AAAATTCCAA TTATGTTGCG TTCAACTTAC 
TGCCTGTTGA ATGGCTTAAC AGATCGTGAT CTTTGTGAGT TAAACGAATG CCCTCTGGAT 
CCTGGTGGCT ACTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCTCA AGAGAAAATG 
GCAACAAACA CAGTGTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACGGGAGAG 
TGTAGGTCAT GTCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATTTGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC AAAAAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCTATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCT 
GACAGAGATA TTTTAGAGCA CATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTTGATGA AGCTTTTGTC ATTCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGCTCAA GAGGGGCAAA GCCTGGAGTC ACCAAAGAGA AAAGAATTAA ATATGCAAAA 
GAGGTCTTAC AAAAAGAAAT GCTCCCTCAT GTTGGCGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGGTA CATGGTTCAT AGGTTGCTTC TAGCAGCATT GGGTAGAAGA 
GAATTAGACG ACAGAGACCA CTATGGAAAC AAAAGATTGG ATCTTGCTGG GCCGCTACTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTGAAAG AAGTGCGGAT CTATGCACAG 
AAGTTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC AAGGATTATA 
TCTGATGGCC TAAAATATTC TTTAGCTACT GGAAACTGGG GTGACCAAAA GAAGGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTATTA AACCGCCTGA CTTTTGCGTC CACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCGATTGGT AGAGACGGCA AGCTGGCCAA ACCAAGACAG 
TTGCATAATA CATTGTGGGG AATGGTGTGT CCTGCTGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCTTCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCT 
GAACAACTTA TGAACACTCT AAGGAAATTA CGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACAGA TGCAGGCCGG 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGACATATT 
GATCAATTGA AAGAGAGAGA GTATAACAAC TACAGTTGGC AAGATCTTGT GGCCAGTGGG 
GTAGTAGAAT ATATTGATAC TCTAGAAGAA GAAACAGTAA TGCTTGCGAT GACTCCGGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATACA CACACTGTGA GATTCATCCG 
TCAATGATTC TTGGCGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAATACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTATAT CACTAACTTC 
CATGTCCGTA TGGATACGTT GGCCCATGTT CTCTATTATC CTCAAAAACC ACTTGTGACT 
ACACGCTCTA TGGAATATCT ACGATTTAGA GAGCTGCCCG CAGGTATCAA CTCAATTGTG 
GCCATTGCAT CGTACACGGG GTATAATCAA GAAGACTCTG TTATCATGAA TCGCTCAGCT 
GTAGACCGAG GCTTTTTCAG GTCTGTTTTC TATCGATCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGTATG 
AGGCATGCCA TCTATGACAA GCTGGATGAT GATGGTTTGA TAGCTCCTGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGGCGCTA TACCAAGAGA GACTGTAGTA CTTTTCTCAG AACTAGTGAG 
ACTGGCATTG TGGATCAGGT TATGGTAACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATCAGGGTGC GCTCTGTTAG AATTCCACAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAGGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT TAATCCCCAC GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTGATTGAGT GTCTTCAAGG GAAGGTATCA GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA TGTGCAAAAG ATTTCTAATC TTTTATCAGA TTATGGTTAC 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATTTTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAT 
TCTCGTGCTA GGGGGCCTAT CCAGATCCTT AACAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA CGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCTAACACA AGGACCCATA CGTACGAGTG CAGGGGTTGC 
CGAAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTATG CGTGTAAACT CTTGTTTCAA 
GAACTTATGT CTATGAGTAT TGCACCTCGA ATGATGAGCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001916826.2
CDS	136..3660
Translation	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	XM_001916791.5
Organism	Equus caballus(horse)
Definition	Equus caballus RNA polymerase II subunit B (POLR2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001916791.5

ORF Insert Sequence:

ATGTATGACG CGGACGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCAGAT 
TTGTGGCAAG AAGCTTGCTG GATTGTAATC AGTTCTTATT TTGACGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCTCCAC CTATAGACCT GCAAGCTGAA GCTCAGCATG CCAGTGGGGA AGTTGAAGAA 
CCGCCAAGAT ATTTGCTGAA GTTTGAACAA ATTTACCTCT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCA AATGAGGCCA GATTAAGAAA TCTCACGTAT 
TCTGCTCCAC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAGAC TTTTATAGGA AAAATTCCAA TTATGTTGCG TTCAACTTAC 
TGCCTGTTGA ATGGCTTAAC AGATCGTGAT CTTTGTGAGT TAAACGAATG CCCTCTGGAT 
CCTGGTGGCT ACTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCTCA AGAGAAAATG 
GCAACAAACA CAGTGTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACGGGAGAG 
TGTAGGTCAT GTCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATTTGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC AAAAAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCTATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCT 
GACAGAGATA TTTTAGAGCA CATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTTGATGA AGCTTTTGTC ATTCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGCTCAA GAGGGGCAAA GCCTGGAGTC ACCAAAGAGA AAAGAATTAA ATATGCAAAA 
GAGGTCTTAC AAAAAGAAAT GCTCCCTCAT GTTGGCGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGGTA CATGGTTCAT AGGTTGCTTC TAGCAGCATT GGGTAGAAGA 
GAATTAGACG ACAGAGACCA CTATGGAAAC AAAAGATTGG ATCTTGCTGG GCCGCTACTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTGAAAG AAGTGCGGAT CTATGCACAG 
AAGTTTATTG ATCGAGGAAA GGATTTTAAC TTGGAGTTGG CAATTAAAAC AAGGATTATA 
TCTGATGGCC TAAAATATTC TTTAGCTACT GGAAACTGGG GTGACCAAAA GAAGGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTATTA AACCGCCTGA CTTTTGCGTC CACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCGATTGGT AGAGACGGCA AGCTGGCCAA ACCAAGACAG 
TTGCATAATA CATTGTGGGG AATGGTGTGT CCTGCTGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCTTCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCT 
GAACAACTTA TGAACACTCT AAGGAAATTA CGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACAGA TGCAGGCCGG 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGACATATT 
GATCAATTGA AAGAGAGAGA GTATAACAAC TACAGTTGGC AAGATCTTGT GGCCAGTGGG 
GTAGTAGAAT ATATTGATAC TCTAGAAGAA GAAACAGTAA TGCTTGCGAT GACTCCGGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATACA CACACTGTGA GATTCATCCG 
TCAATGATTC TTGGCGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAATACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTATAT CACTAACTTC 
CATGTCCGTA TGGATACGTT GGCCCATGTT CTCTATTATC CTCAAAAACC ACTTGTGACT 
ACACGCTCTA TGGAATATCT ACGATTTAGA GAGCTGCCCG CAGGTATCAA CTCAATTGTG 
GCCATTGCAT CGTACACGGG GTATAATCAA GAAGACTCTG TTATCATGAA TCGCTCAGCT 
GTAGACCGAG GCTTTTTCAG GTCTGTTTTC TATCGATCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGTATG 
AGGCATGCCA TCTATGACAA GCTGGATGAT GATGGTTTGA TAGCTCCTGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGGCGCTA TACCAAGAGA GACTGTAGTA CTTTTCTCAG AACTAGTGAG 
ACTGGCATTG TGGATCAGGT TATGGTAACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATCAGGGTGC GCTCTGTTAG AATTCCACAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAGGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT TAATCCCCAC GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTGATTGAGT GTCTTCAAGG GAAGGTATCA GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA TGTGCAAAAG ATTTCTAATC TTTTATCAGA TTATGGTTAC 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATTTTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAT 
TCTCGTGCTA GGGGGCCTAT CCAGATCCTT AACAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA CGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCTAACACA AGGACCCATA CGTACGAGTG CAGGGGTTGC 
CGAAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTATG CGTGTAAACT CTTGTTTCAA 
GAACTTATGT CTATGAGTAT TGCACCTCGA ATGATGAGCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLR2B ( XM_001916791.4 ) cDNA ORF clone, Equus caballus(horse) -> XP_001916826.1 Equus caballus polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), mRNA.

Price & Availability↑

CloneID	OHb18922
Clone ID Related Accession (Same CDS sequence)	XM_001916791.4
Accession Version	XM_001916791.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-11-19
Organism	Equus caballus(horse)
Product	LOW QUALITY PROTEIN: DNA-directed RNA polymerase II subunit RPB2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867411.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001916791.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGTATGACG CGGACGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCAGAT 
TTGTGGCAAG AAGCTTGCTG GATTGTAATC AGTTCTTATT TTGACGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCTCCAC CTATAGACCT GCAAGCTGAA GCTCAGCATG CCAGTGGGGA AGTTGAAGAA 
CCGCCAAGAT ATTTGCTGAA GTTTGAACAA ATTTACCTCT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCA AATGAGGCCA GATTAAGAAA TCTCACGTAT 
TCTGCTCCAC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAGAC TTTTATAGGA AAAATTCCAA TTATGTTGCG TTCAACTTAC 
TGCCTGTTGA ATGGCTTAAC AGATCGTGAT CTTTGTGAGT TAAACGAATG CCCTCTGGAT 
CCTGGTGGCT ACTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCTCA AGAGAAAATG 
GCAACAAACA CAGTGTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACGGGAGAG 
TGTAGGTCAT GTCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATTTGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC AAAAAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCTATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCT 
GACAGAGATA TTTTAGAGCA CATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTTGATGA AGCTTTTGTC ATTCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGCTCAA GAGGGGCAAA GCCTGGAGTC ACCAAAGAGA AAAGAATTAA ATATGCAAAA 
GAGGTCTTAC AAAAAGAAAT GCTCCCTCAT GTTGGCGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGGTA CATGGTTCAT AGGTTGCTTC TAGCAGCATT GGGTAGAAGA 
GAATTAGACG ACAGAGACCA CTATGGAAAC AAAAGATTGG ATCTTGCTGG GCCGCTACTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTGAAAG AAGTGCGGAT CTATGCACAG 
AAGTTTATTG ATCGAGGAAA GGATTTTAAC TTGCAGTTGG CAATTAAAAC AAGGATTATA 
TCTGATGGCC TAAAATATTC TTTAGCTACT GGAAACTGGG GTGACCAAAA GAAGGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTATTA AACCGCCTGA CTTTTGCGTC CACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCGATTGGT AGAGACGGCA AGCTGGCCAA ACCAAGACAG 
TTGCATAATA CATTGTGGGG AATGGTGTGT CCTGCTGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCTTCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCT 
GAACAACTTA TGAACACTCT AAGGAAATTA CGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACAGA TGCAGGCCGG 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGACATATT 
GATCAATTGA AAGAGAGAGA GTATAACAAC TACAGTTGGC AAGATCTTGT GGCCAGTGGG 
GTAGTAGAAT ATATTGATAC TCTAGAAGAA GAAACAGTAA TGCTTGCGAT GACTCCGGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATACA CACACTGTGA GATTCATCCG 
TCAATGATTC TTGGCGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAATACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTATAT CACTAACTTC 
CATGTCCGTA TGGATACGTT GGCCCATGTT CTCTATTATC CTCAAAAACC ACTTGTGACT 
ACACGCTCTA TGGAATATCT ACGATTTAGA GAGCTGCCCG CAGGTATCAA CTCAATTGTG 
GCCATTGCAT CGTACACGGG GTATAATCAA GAAGACTCTG TTATCATGAA TCGCTCAGCT 
GTAGACCGAG GCTTTTTCAG GTCTGTTTTC TATCGATCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGTATG 
AGGCATGCCA TCTATGACAA GCTGGATGAT GATGGTTTGA TAGCTCCTGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGGCGCTA TACCAAGAGA GACTGTAGTA CTTTTCTCAG AACTAGTGAG 
ACTGGCATTG TGGATCAGGT TATGGTAACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATCAGGGTGC GCTCTGTTAG AATTCCACAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAGGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT TAATCCCCAC GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTGATTGAGT GTCTTCAAGG GAAGGTATCA GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA TGTGCAAAAG ATTTCTAATC TTTTATCAGA TTATGGTTAC 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATTTTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAT 
TCTCGTGCTA GGGGGCCTAT CCAGATCCTT AACAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA CGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCTAACACA AGGACCCATA CGTACGAGTG CAGGGGTTGC 
CGAAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTATG CGTGCAAACT CTTGTTTCAA 
GAACTTATGT CTATGAGTAT TGCACCTCGA ATGATGAGCG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001916826.1
CDS	118..3642
Translation	MYDADEDMQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGEVEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKTFIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAYTGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEHIIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFCETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGKDFNLQLAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAKPRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKIFVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKKRHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVCASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGINSIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYKFCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDDKIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAIANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

Target ORF information:

RefSeq Version	XM_001916791.4
Organism	Equus caballus(horse)
Definition	Equus caballus polymerase (RNA) II (DNA directed) polypeptide B, 140kDa (POLR2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001916791.4

ORF Insert Sequence:

ATGTATGACG CGGACGAGGA TATGCAATAT GATGAGGATG ATGATGAAAT CACCCCAGAT 
TTGTGGCAAG AAGCTTGCTG GATTGTAATC AGTTCTTATT TTGACGAGAA AGGCTTGGTC 
AGACAACAGC TGGATTCTTT TGATGAGTTT ATTCAGATGT CTGTTCAAAG AATTGTGGAA 
GATGCTCCAC CTATAGACCT GCAAGCTGAA GCTCAGCATG CCAGTGGGGA AGTTGAAGAA 
CCGCCAAGAT ATTTGCTGAA GTTTGAACAA ATTTACCTCT CCAAGCCTAC CCATTGGGAA 
AGAGATGGTG CTCCTTCACC AATGATGCCA AATGAGGCCA GATTAAGAAA TCTCACGTAT 
TCTGCTCCAC TTTATGTTGA TATAACAAAA ACAGTCATTA AAGAAGGTGA AGAACAACTT 
CAGACTCAGC ATCAGAAGAC TTTTATAGGA AAAATTCCAA TTATGTTGCG TTCAACTTAC 
TGCCTGTTGA ATGGCTTAAC AGATCGTGAT CTTTGTGAGT TAAACGAATG CCCTCTGGAT 
CCTGGTGGCT ACTTCATTAT TAATGGATCA GAAAAGGTTC TGATTGCTCA AGAGAAAATG 
GCAACAAACA CAGTGTATGT GTTTGCCAAA AAGGATTCTA AATATGCCTA CACGGGAGAG 
TGTAGGTCAT GTCTGGAGAA TTCTTCCCGA CCCACCAGTA CTATTTGGGT TAGCATGCTG 
GCAAGAGGAG GACAGGGTGC AAAAAAGAGT GCTATTGGTC AGCGCATTGT GGCAACTCTA 
CCATATATCA AGCAAGAAGT TCCTATCATT ATTGTGTTCA GAGCATTAGG TTTTGTGTCT 
GACAGAGATA TTTTAGAGCA CATTATTTAT GATTTTGAAG ATCCAGAGAT GATGGAAATG 
GTTAAACCTT CTCTTGATGA AGCTTTTGTC ATTCAAGAAC AGAATGTTGC ACTAAATTTC 
ATTGGCTCAA GAGGGGCAAA GCCTGGAGTC ACCAAAGAGA AAAGAATTAA ATATGCAAAA 
GAGGTCTTAC AAAAAGAAAT GCTCCCTCAT GTTGGCGTCA GTGATTTTTG TGAGACCAAA 
AAAGCCTATT TCTTGGGGTA CATGGTTCAT AGGTTGCTTC TAGCAGCATT GGGTAGAAGA 
GAATTAGACG ACAGAGACCA CTATGGAAAC AAAAGATTGG ATCTTGCTGG GCCGCTACTT 
GCATTCTTAT TTAGAGGTAT GTTTAAGAAT TTGCTGAAAG AAGTGCGGAT CTATGCACAG 
AAGTTTATTG ATCGAGGAAA GGATTTTAAC TTGCAGTTGG CAATTAAAAC AAGGATTATA 
TCTGATGGCC TAAAATATTC TTTAGCTACT GGAAACTGGG GTGACCAAAA GAAGGCTCAT 
CAAGCCAGAG CTGGAGTATC TCAGGTATTA AACCGCCTGA CTTTTGCGTC CACTCTTTCT 
CACCTGCGTC GTTTAAATTC TCCGATTGGT AGAGACGGCA AGCTGGCCAA ACCAAGACAG 
TTGCATAATA CATTGTGGGG AATGGTGTGT CCTGCTGAGA CCCCAGAGGG CCATGCTGTA 
GGACTTGTGA AGAATTTAGC CCTGATGGCT TATATTTCAG TTGGATCTCA ACCTTCTCCA 
ATTCTGGAAT TTTTAGAAGA ATGGAGTATG GAAAATTTAG AAGAAATTTC TCCTGCAGCT 
ATTGCTGATG CAACCAAGAT TTTTGTTAAT GGCTGCTGGG TTGGAATACA TAAAGATCCT 
GAACAACTTA TGAACACTCT AAGGAAATTA CGACGTCAGA TGGACATCAT TGTGTCTGAA 
GTTTCTATGA TCAGAGATAT TCGAGAGAGG GAGATTCGGA TCTATACAGA TGCAGGCCGG 
ATTTGTAGAC CACTTCTGAT TGTGGAAAAA CAAAAGCTAC TTTTGAAGAA GAGACATATT 
GATCAATTGA AAGAGAGAGA GTATAACAAC TACAGTTGGC AAGATCTTGT GGCCAGTGGG 
GTAGTAGAAT ATATTGATAC TCTAGAAGAA GAAACAGTAA TGCTTGCGAT GACTCCGGAT 
GATTTACAGG AGAAAGAAGT AGCTTATTGT TCCACATACA CACACTGTGA GATTCATCCG 
TCAATGATTC TTGGCGTCTG TGCATCTATT ATTCCCTTTC CTGATCATAA CCAGTCCCCT 
AGAAATACAT ACCAGTCTGC TATGGGTAAG CAGGCTATGG GAGTTTATAT CACTAACTTC 
CATGTCCGTA TGGATACGTT GGCCCATGTT CTCTATTATC CTCAAAAACC ACTTGTGACT 
ACACGCTCTA TGGAATATCT ACGATTTAGA GAGCTGCCCG CAGGTATCAA CTCAATTGTG 
GCCATTGCAT CGTACACGGG GTATAATCAA GAAGACTCTG TTATCATGAA TCGCTCAGCT 
GTAGACCGAG GCTTTTTCAG GTCTGTTTTC TATCGATCAT ACAAAGAACA GGAGTCTAAA 
AAAGGATTTG ATCAAGAAGA AGTTTTTGAG AAGCCTACAC GTGAAACATG CCAGGGTATG 
AGGCATGCCA TCTATGACAA GCTGGATGAT GATGGTTTGA TAGCTCCTGG GGTTCGTGTA 
TCAGGAGATG ATGTTATTAT AGGCAAAACA GTCACCTTGC CTGAAAATGA AGATGAATTG 
GAGAGCACCA ATAGGCGCTA TACCAAGAGA GACTGTAGTA CTTTTCTCAG AACTAGTGAG 
ACTGGCATTG TGGATCAGGT TATGGTAACT CTCAACCAGG AAGGATATAA ATTTTGTAAA 
ATCAGGGTGC GCTCTGTTAG AATTCCACAG ATTGGAGACA AATTTGCTAG CCGACATGGT 
CAAAAGGGTA CTTGTGGTAT TCAGTATAGA CAGGAGGATA TGCCTTTCAC CTGTGAAGGT 
ATCACCCCTG ATATCATCAT TAATCCCCAC GCCATCCCCT CTCGTATGAC TATTGGTCAC 
TTGATTGAGT GTCTTCAAGG GAAGGTATCA GCTAACAAGG GTGAAATTGG TGATGCCACT 
CCATTTAATG ATGCTGTTAA TGTGCAAAAG ATTTCTAATC TTTTATCAGA TTATGGTTAC 
CATCTCAGAG GAAATGAGGT CCTGTACAAT GGGTTCACTG GTCGAAAAAT CACATCACAA 
ATTTTTATTG GCCCCACTTA TTACCAGCGT TTGAAGCATA TGGTGGATGA TAAGATTCAT 
TCTCGTGCTA GGGGGCCTAT CCAGATCCTT AACAGACAGC CCATGGAGGG TAGATCTCGT 
GATGGTGGCC TGCGTTTTGG AGAAATGGAA CGAGATTGTC AGATTGCCCA TGGAGCAGCC 
CAGTTTTTAA GGGAAAGATT GTTTGAGGCA TCAGATCCAT ATCAGGTTCA CGTTTGCAAT 
CTTTGTGGAA TAATGGCGAT TGCTAACACA AGGACCCATA CGTACGAGTG CAGGGGTTGC 
CGAAATAAAA CCCAGATTTC TTTGGTGCGA ATGCCTTATG CGTGCAAACT CTTGTTTCAA 
GAACTTATGT CTATGAGTAT TGCACCTCGA ATGATGAGCG TTTAA