NOS1 cDNA ORF clone, Equus caballus(horse)

The following NOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHb69676 XM_001914970.5
Latest version!
Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb69676 XM_023647063.1
Latest version!
Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb69677 XM_023647064.1
Latest version!
Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHb24818 XM_001914970.4
Latest version!
Equus caballus nitric oxide synthase 1 (neuronal) (NOS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHb69676
    Clone ID Related Accession (Same CDS sequence) XM_023647063.1 , XM_001914970.5
    Accession Version XM_023647063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CGGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACCG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023502831.1
    CDS602..5008
    Translation

    Target ORF information:

    RefSeq Version XM_023647063.1
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023647063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CGGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACCG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb69676
    Clone ID Related Accession (Same CDS sequence) XM_023647063.1 , XM_001914970.5
    Accession Version XM_001914970.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product nitric oxide synthase, brain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 23, 2018 this sequence version replaced XM_001914970.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CGGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACCG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001915005.3
    CDS768..5174
    Translation

    Target ORF information:

    RefSeq Version XM_001914970.5
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001914970.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCCCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CGGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCCC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACCG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb69677
    Clone ID Related Accession (Same CDS sequence) XM_023647064.1
    Accession Version XM_023647064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4305bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-01-22
    Organism Equus caballus(horse)
    Product nitric oxide synthase, brain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_009151.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Equus caballus Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGAGCTACAA GGTCCGATTC AACAGTGTTT CCTCCTATTC CGACTCCCGC
    AAATCATCGG GCGATGGGCC AGACCTCAGA GACAACTTTG AGAGTACCGG GCCCCTGGCG
    AATGTGAGGT TCTCAGTGTT CGGCCTTGGC TCGCGGGCCT ACCCTCACTT CTGCGCCTTT
    GGACACGCCG TGGACACCCT CCTGGAAGAG CTGGGAGGAG AGAGGATCCT GAAGATGAGG
    GAAGGCGACG AGCTCTGTGG GCAGGAAGAA GCTTTCAGGA CCTGGGCCAA GAAGGTCTTT
    AAGGCGGCCT GCGATGTGTT CTGCGTGGGA GACGATGTCA ACATTGAGAA GGCAAACAAC
    TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAC CTATGTGGCT
    GAAGCTCCGG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGGGT CTCAGCCGCT
    CGACTCCTCA GCCGCCAAAA CCTTCAGAGT CCTAAATCCA GCCGATCAAC CATCTTCGTG
    CGTCTCCACA CCAATGGGAA TCAGGAGCTG CAGTACCAGC CCGGGGACCA CCTGGGCGTC
    TTCCCCGGCA ACCACGAGGA CCTCGTGAAC GCCCTGATCG AGCGGCTGGA GGACGCACCC
    CCTGTCAACC AGCTGGTCAA AGTGGAGCTC CTGGAGGAGC GGAACACGGC TTTGGGAGTG
    ATCAGTAACT GGACGGACGA GCACCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ATATCACCAC ACCCCCAACG CCGCTCCAGC TGCAGCAGTT CGCCTCCCTG
    GCCACGAGCG AGAAAGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAATAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCGTCC
    ATCCAGATGC CCGCCACCCT GCTCCTCACC CAGCTGTCTC TCCTGCAACC TCGTTACTAT
    TCCATCAGTT CTTCCCCAGA CATGTACCCG GATGAGGTGC ACCTCACCGT GGCCGTCGTC
    TCCTACCGCA CGCGAGATGG AGAAGGGCCA ATTCACCACG GCGTGTGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA AGGGGTCCCC TGTTTCGTGA GAGGCGCGCC CAGCTTCCGC
    CTGCCCCGAA ATCCCCTGGT GCCATGCATT CTGGTTGGCC CGGGCACTGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAGCA GCGGCAGTTT GATATCCAAC ACAAAGGAAT GAGTCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG
    ACCCTACAGG CCAAGAGCAA GGGGGTCTTC AGAGAACTGT ACACGGCCTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC GTCCTGCAGG AGCAGCTGGC GGAAACCGTG
    TACCGAGCCC TGAAGGAGCA AGGAGGCCAC ATTTACGTCT GTGGGGACGT CACGATGGCC
    GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCGGTGGAG
    GACGCCGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTATCATGA GGATATTTTC
    GGGGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC
    ATCGAGGAGA GCAAAAAAGA CACGGACGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_023502832.1
    CDS1..4305
    Translation

    Target ORF information:

    RefSeq Version XM_023647064.1
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 1 (NOS1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_023647064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGAGCTACAA GGTCCGATTC AACAGTGTTT CCTCCTATTC CGACTCCCGC
    AAATCATCGG GCGATGGGCC AGACCTCAGA GACAACTTTG AGAGTACCGG GCCCCTGGCG
    AATGTGAGGT TCTCAGTGTT CGGCCTTGGC TCGCGGGCCT ACCCTCACTT CTGCGCCTTT
    GGACACGCCG TGGACACCCT CCTGGAAGAG CTGGGAGGAG AGAGGATCCT GAAGATGAGG
    GAAGGCGACG AGCTCTGTGG GCAGGAAGAA GCTTTCAGGA CCTGGGCCAA GAAGGTCTTT
    AAGGCGGCCT GCGATGTGTT CTGCGTGGGA GACGATGTCA ACATTGAGAA GGCAAACAAC
    TCCCTCATCA GCAATGACCG CAGCTGGAAG AGGAACAAGT TCCGCCTCAC CTATGTGGCT
    GAAGCTCCGG AACTCACACA AGGTCTATCC AATGTCCACA AAAAGCGGGT CTCAGCCGCT
    CGACTCCTCA GCCGCCAAAA CCTTCAGAGT CCTAAATCCA GCCGATCAAC CATCTTCGTG
    CGTCTCCACA CCAATGGGAA TCAGGAGCTG CAGTACCAGC CCGGGGACCA CCTGGGCGTC
    TTCCCCGGCA ACCACGAGGA CCTCGTGAAC GCCCTGATCG AGCGGCTGGA GGACGCACCC
    CCTGTCAACC AGCTGGTCAA AGTGGAGCTC CTGGAGGAGC GGAACACGGC TTTGGGAGTG
    ATCAGTAACT GGACGGACGA GCACCGCCTC CCGCCCTGCA CCATCTTCCA GGCCTTCAAG
    TACTACCTGG ATATCACCAC ACCCCCAACG CCGCTCCAGC TGCAGCAGTT CGCCTCCCTG
    GCCACGAGCG AGAAAGAGAA GCAGCGTCTG CTGGTCCTCA GCAAGGGTTT GCAGGAATAC
    GAGGAATGGA AATGGGGCAA GAACCCCACC ATCGTGGAGG TGCTGGAGGA GTTCCCGTCC
    ATCCAGATGC CCGCCACCCT GCTCCTCACC CAGCTGTCTC TCCTGCAACC TCGTTACTAT
    TCCATCAGTT CTTCCCCAGA CATGTACCCG GATGAGGTGC ACCTCACCGT GGCCGTCGTC
    TCCTACCGCA CGCGAGATGG AGAAGGGCCA ATTCACCACG GCGTGTGCTC CTCCTGGCTC
    AACCGGATAC AGGCTGACGA AGGGGTCCCC TGTTTCGTGA GAGGCGCGCC CAGCTTCCGC
    CTGCCCCGAA ATCCCCTGGT GCCATGCATT CTGGTTGGCC CGGGCACTGG CATTGCCCCT
    TTCCGAAGCT TCTGGCAGCA GCGGCAGTTT GATATCCAAC ACAAAGGAAT GAGTCCCTGC
    CCCATGGTCC TGGTCTTCGG GTGCCGGCAG TCCAAGATAG ACCACATCTA CAAGGAGGAG
    ACCCTACAGG CCAAGAGCAA GGGGGTCTTC AGAGAACTGT ACACGGCCTA CTCCCGGGAG
    CCAGACAAAC CAAAGAAGTA CGTGCAGGAC GTCCTGCAGG AGCAGCTGGC GGAAACCGTG
    TACCGAGCCC TGAAGGAGCA AGGAGGCCAC ATTTACGTCT GTGGGGACGT CACGATGGCC
    GCCGATGTCC TCAAAGCCAT CCAGCGCATC ATGACCCAGC AGGGGAAACT CTCGGTGGAG
    GACGCCGGCG TGTTCATCAG CAGGCTGAGG GATGACAACC GGTATCATGA GGATATTTTC
    GGGGTCACCC TGCGAACGTA CGAAGTGACC AACCGCCTTA GATCTGAATC CATTGCCTTC
    ATCGAGGAGA GCAAAAAAGA CACGGACGAG GTTTTCAGCT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHb24818
    Clone ID Related Accession (Same CDS sequence) XM_001914970.4
    Accession Version XM_001914970.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4407bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-11-19
    Organism Equus caballus(horse)
    Product LOW QUALITY PROTEIN: nitric oxide synthase, brain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001867429.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Nov 20, 2015 this sequence version replaced XM_001914970.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Equus caballus Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCTCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CCGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCTC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACGG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001915005.3
    CDS772..5178
    Translation

    Target ORF information:

    RefSeq Version XM_001914970.4
    Organism Equus caballus(horse)
    Definition Equus caballus nitric oxide synthase 1 (neuronal) (NOS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001914970.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGAATC ACATGTTTAG TGTTCAACAA ATCCAACCCA ATGTCATCTC CGTCCGCCTC 
    TTCAAGCGCA AAGTGGGGGG CCTGGGATTC TTGGTGAAGG AGCGGGTGAG CAAGCCGCCC
    GTGATCATCT CAGACCTGAT TCGAGGGGGT GCCGCGGAGC AGAGCGGCCT CATCCAGGCT
    GGAGACATCA TTCTTGCTGT CAATGGCCAG CCCCTGGTGG ACCTGAGCTA TGACAGCGCG
    CTGGAGGTCC TCAGGGGCAT TGCCTCCGAG ACCCACGTGG TCCTCATTCT GAGGGGTCCT
    GAGGGCTTCA CCACGCACCT GGAGACCACC TTCACTGGAG ACGGGACCCC TAAGACCATC
    CGTGTGACAC GGCCCCTGGG TCCCCCCACT GCAGCCATCG ATCTGTCCCA CCAGCCATCG
    GCCAGCAAGG AGCAGCCACT CACCGTGGAT GGGGCCACAG GTCCTGGCAA TGGGCCACAG
    CATGCCCAAG ACAATAGACA GGAAGCTGGC TCGCTCTGCC ACACCAACGG CCTGGCTCCC
    AGGCCCTCAA GCCAGGAGCC TGCCAAGAAA AGTGCCGGGA TCGCCCTCCA GGGCAGCGGG
    AAGAACAATG AACTGCTCAG AGAGATAGAG CCTGTGCTGA GCCTTCTCGC CAGTGGGGGC
    AAAGGCATCA ATGGAGGGGG ACCTGCCAAG ATGGAGACAA AAGATGCAGA AGTCCAGGTT
    GACAGAGACC TGGATGGCAA GTCACACAAA CCACTGCCCC TCGGCGTGGA GAACGACCGA
    GTCTTCAACG ACCTGTGGGG GAAGGGCAAT GTGCCCGTCG TCCTCAACAA CCCATATTCA
    GAGAAGGAGC AGCCCCCTGC CTCGGGAAAA CAGTCCCCCA CAAAGAATGG CAGCCCCTCC
    AAGTGCCCGC GCTTCCTCAA GGTCAAGAAC TGGGAGACGG ACGTGGTTCT CACTGACACC
    CTCCACCTCA AGAGCACGTT GGAAACGGGA TGCACCGAGC ACATCTGTAT GGGCTCCATC
    ATGCTCCCTT CTCAGCACAT ACGAAGGCCC GAGGACGTCC GCACAAAGCA ACAGCTCTTC
    CCTCTCGCCA AAGAATTTAT TGATCAGTAC TACTCCTCAA TTAAAAGATC TGGCTCCAAA
    GCCCACATGG AGCGGCTGGA AGAGGTGAAC AAAGAGATCG AAACCACCAG CACCTACCAG
    CTCAGAGACA CGGAGCTCAT CTACGGGGCG AAGCACGCCT GGCGGAACGC CTCCCGCTGC
    GTCGGCAGGA TCCAGTGGTC CAAGCTGCAG GTGTTCGATG CCCGCGACTG CACCACGGCT
    CATGGGATGT TCAACTACAT CTGTAATCAT ATCAAGTATG CCACCAACAA AGGGAACCTC
    AGGTCTGCCA TCACCATATT TCCCCAGAGG ACTGACGGCA AGCACGACTT CCGAGTCTGG
    AACTCCCAGC TCATCCGCTA TGCCGGCTAC AAGCAGCCCG ACGGCTCCAT CCTCGGGGAT
    CCAGCCAATG TGGAGTTCAC AGAGATCTGC ACACAGCAGG GCTGGAAACC CCCCAGAGGC
    CGCTTCGACG TCCTGCCACT CCTGCTCCAG GCCAACGGCA ACGACCCCGA GCTCTTCCAG
    ATCCCTCCAG AGCTGGTGCT GGAAGTGCCC ATCAGACACC CCAAGTTTGA GTGGTTCAAG
    GACCTGGGAC TGAAGTGGTA CGGCCTGCCC GCTGTGTCCA ACATGCTTCT GGAGATTGGC
    GGCCTGGAGT TCAGTGCCTG TCCCTTCAGC GGCTGGTACA TGGGCACGGA GATTGGCGTC
    CGTGACTACT GTGACAACTC ACGATACAAC ATCCTGGAGG AAGTGGCCAA GAAGATGAAC
    CTGGACCTGA GGAAGACGTC CTCCCTGTGG AAGGACCAGG CGCTGGTGGA GATCAACATT
    GCCGTTCTCT ACAGCTTCCA GAGTGACAAA GTGACCATCG TTGACCACCA CTCTGCCACT
    GAGTCCTTCA TCAAGCACAT GGAGAATGAA TACCGCTGCC GGGGGGGCTG CCCCGCTGAC
    TGGGTGTGGA TTGTGCCCCC CATGTCTGGA AGCATCACCC CTGTCTTCCA CCAGGAGATG
    CTCAACTACC GGCTCACCCC CTCCTTCGAA TACCAGCCTG ATCCTTGGAA CACCCATGTC
    TGGAAGGGTA CCAACGGGAC CCCCACAAAG CGGCGAGCCA TCGGCTTCAA GAAGCTGGCA
    GAAGCCGTCA AGTTCTCGGC CAAGCTGATG GGGCAGGCAA TGGCCAAAAG GGTCAAGGCG
    ACCATCCTTT ACGCCACAGA GACGGGCAAG TCGCAGGCCT ACGCCAAAAC CTTGTGTGAG
    ATCTTCAAAC ACGCCTTTGA TGCCAAGGTG ATGTCCATGG AAGAATATGA CATTGTGCAC
    CTGGAACATG AAACTCTGGT CCTGGTGGTT ACCAGCACCT TCGGCAATGG AGACCCCCCT
    GAGAACGGAG AGAAATTCGG CTGTGCTTTG ATGGAAATGA GACACCCCAA CTCTGTGCAC
    GAGGAAAGGA AGTACCCGGA ACCCTTGCGT TTCTTTCCCC GTAAAGGGCC TCCCCTCCCC
    CGAGGTGACA CAGAAGTCCA TGGTCTGGCT GCAGCCCGTG ACAGCCAGCG CAGGAGCTAC
    AAGGTCCGAT TCAACAGTGT TTCCTCCTAT TCCGACTCCC GCAAATCATC GGGCGATGGG
    CCAGACCTCA GAGACAACTT TGAGAGTACC GGGCCCCTGG CGAATGTGAG GTTCTCAGTG
    TTCGGCCTTG GCTCGCGGGC CTACCCTCAC TTCTGCGCCT TTGGACACGC CGTGGACACC
    CTCCTGGAAG AGCTGGGAGG AGAGAGGATC CTGAAGATGA GGGAAGGCGA CGAGCTCTGT
    GGGCAGGAAG AAGCTTTCAG GACCTGGGCC AAGAAGGTCT TTAAGGCGGC CTGCGATGTG
    TTCTGCGTGG GAGACGATGT CAACATTGAG AAGGCAAACA ACTCTCTCAT CAGCAATGAC
    CGCAGCTGGA AGAGGAACAA GTTCCGCCTC ACCTATGTGG CTGAAGCTCC GGAACTCACA
    CAAGGTCTAT CCAATGTCCA CAAAAAGCGG GTCTCAGCCG CTCGACTCCT CAGCCGCCAA
    AACCTTCAGA GTCCTAAATC CAGCCGATCA ACCATCTTCG TGCGTCTCCA CACCAATGGG
    AATCAGGAGC TGCAGTACCA GCCCGGGGAC CACCTGGGCG TCTTCCCCGG CAACCACGAG
    GACCTCGTGA ACGCCCTGAT CGAGCGGCTG GAGGACGCAC CCCCTGTCAA CCAGCTGGTC
    AAAGTGGAGC TCCTGGAGGA GCGGAACACG GCTTTGGGAG TGATCAGTAA CTGGACGGAC
    GAGCACCGCC TCCCGCCCTG CACCATCTTC CAGGCCTTCA AGTACTACCT GGATATCACC
    ACACCCCCAA CGCCGCTCCA GCTGCAGCAG TTCGCCTCCC TGGCCACGAG CGAGAAAGAG
    AAGCAGCGTC TGCTGGTCCT CAGCAAGGGT TTGCAGGAAT ACGAGGAATG GAAATGGGGC
    AAGAACCCCA CCATCGTGGA GGTGCTGGAG GAGTTCCCGT CCATCCAGAT GCCCGCCACC
    CTGCTCCTCA CCCAGCTGTC TCTCCTGCAA CCTCGTTACT ATTCCATCAG TTCTTCCCCA
    GACATGTACC CCGATGAGGT GCACCTCACC GTGGCCGTCG TCTCCTACCG CACGCGAGAT
    GGAGAAGGGC CAATTCACCA CGGCGTGTGC TCCTCCTGGC TCAACCGGAT ACAGGCTGAC
    GAAGGGGTCC CCTGTTTCGT GAGAGGCGCG CCCAGCTTCC GCCTGCCCCG AAATCCCCTG
    GTGCCATGCA TTCTGGTTGG CCCGGGCACT GGCATTGCTC CTTTCCGAAG CTTCTGGCAG
    CAGCGGCAGT TTGATATCCA ACACAAAGGA ATGAGTCCCT GCCCCATGGT CCTGGTCTTC
    GGGTGCCGGC AGTCCAAGAT AGACCACATC TACAAGGAGG AGACCCTACA GGCCAAGAGC
    AAGGGGGTCT TCAGAGAACT GTACACGGCC TACTCCCGGG AGCCAGACAA ACCAAAGAAG
    TACGTGCAGG ACGTCCTGCA GGAGCAGCTG GCGGAAACGG TGTACCGAGC CCTGAAGGAG
    CAAGGAGGCC ACATTTACGT CTGTGGGGAC GTCACGATGG CCGCCGATGT CCTCAAAGCC
    ATCCAGCGCA TCATGACCCA GCAGGGGAAA CTCTCGGTGG AGGACGCCGG CGTGTTCATC
    AGCAGGCTGA GGGATGACAA CCGGTATCAT GAGGATATTT TCGGGGTCAC CCTGCGAACG
    TACGAAGTGA CCAACCGCCT TAGATCTGAA TCCATTGCCT TCATCGAGGA GAGCAAAAAA
    GACACGGACG AGGTTTTCAG CTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.