Dsim\GD24961 cDNA ORF clone, Drosophila simulans

Gene
Clones
gRNAs

Summary

Gene Symbol	Dsim\GD24961
Entrez Gene ID	6736049
Full Name	GD24961 gene product from transcript GD24961-RE
Synonyms	Dsim_GD24961,GD24961,dsim_GLEANR_8963
Gene Type	protein-coding
Organism	Drosophila simulans

mRNA and Protein(s)

mRNA	Protein	Name
XM_016181226.1	XP_016029446.1	uncharacterized protein, isoform A
XM_016181232.1	XP_016029452.1	uncharacterized protein, isoform G
XM_016181231.1	XP_016029451.1	uncharacterized protein, isoform F
XM_016181227.1	XP_016029447.1	uncharacterized protein, isoform B
XM_016181228.1	XP_016029448.1	uncharacterized protein, isoform C
XM_016181229.1	XP_016029449.1	uncharacterized protein, isoform D
XM_016181230.1	XP_016029450.1	uncharacterized protein, isoform E

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Hu TT, Eisen MB, Thornton KR, Andolfatto P
Genome research23(1)89-98(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dsim\GD24961 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dsim\GD24961 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODg22156	XM_016181227.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODg22159	XM_016181231.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODg22159	XM_016181230.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODg22160	XM_016181232.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODg22157	XM_016181228.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODg22157	XM_016181229.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODg22158	XM_016181226.1 Latest version!	Drosophila simulans uncharacterized protein, transcript variant A (Dsim\GD24961), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dsim\GD24961 ( XM_016181227.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029447.1 Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD24961), mRNA.

Price & Availability↑

CloneID	ODg22156
Clone ID Related Accession (Same CDS sequence)	XM_016181227.1
Accession Version	XM_016181227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGAAGCT TAAACGCCAA GTGAAGCGGC GCTCGCTCTA TCAGAGGGAA 
CCCCGCAACT CGGCGCTCCA GCTCGACCTG GACGATGACT CTGCTTCGCT GCAGAGCCAA 
GACATTGACA AACTTCCAGG TACACCTACA TCCATTTTGC GGGATGCCAA AGAGCAGTCC 
CAACCAAGTA GCAGTTTACC AATCGCAGCA GCGGCAACTG CAGCAGCAGC AGCTACAACC 
ACAACTAAAG CAACCACTCC GGACAGACCA AACCACACAA AGGATGACAA TGCTGAAGCG 
GCAGCCCAGC AGCCGCCGCA TACCAAAGTG CCTGCTCCAG CGCCGCCGTC GTCCCAACAA 
ACGCCACCAC CGCAAGTTCA GCAGCCACCT ACACCACCAG CCCAACCCAC AGCGGCTGTG 
CTGCAAAAGA AACCCGAAGA TGTGGCAGCT GTTGCAGAAA CATCTGAGAA AACCGAATCC 
GACAAGAAGC ACTTTGTCAA AAAGGGTAAG GCCCCGCCCC CGCCGTCCCC ATTGGGTCTT 
GCAAATGCCA AGCCGGCTGC CAGCGACTCC CAAACGTCTC CCAAAAAACT GGCCACTCCC 
GAGCCCACTT CTCCAGTCAC CACGGCTATT GAGGTGGCCA TTGGCCAAGA GGCGATGGAG 
CCGAAACCGC AGCCGCCATC ACCCACAGCC TCCTCCATTG TGTCTGTGCA ATCGGTGGCC 
TCTTCCAGCT CCTCGGGCAG TGTTTCAAAT GCTGTTCTCA GCTCGAGCAC TTCCCTTATT 
ACCATCAACA GCGATCCACC AACACCGCAT CATCACCAAC CGCTGCCACC TCAACCGCAG 
CACTTGGTTT TGCCAAACAG CTTGGAGTCG GTGAGTCAGA TAACAGTCGT GACCAGCACC 
CATCCGCCGG TGATCATCGA CAACTCGGTG GTGCCACCGC AAAACGAGGT GATCATCGTT 
TCCAATGATA TGAACAAGAG CACTCACCTG CATGAATCCT CAACGGACGA TGATTTTCCA 
TCGCTGGACG ACAGTTTGGG TGATGCCACC ACGCCGCCCC ACAAGCAATC CTCAATGATA 
TTGGCTGTTA ATGAGCCAGC AGGTGTGGTT CCCGCTCCTC CGTCGCAACC GCAAACGTCT 
TCCACTGTTC ACGCACGCAA ACTGGATGAA AGTGAGGTGT TAATAGTCAG TCCGTCCTAC 
GCGGATGATG ACTCGGCCTA TAACACGGCG TCTGGCAGTC ATAGCCACGA TCACAGTGAC 
CATCTGATGG ACACGAGCCA CGTCTCCGTG GTCACCGTGG GCGATGAGGG AGTTAAGGTG 
AAGGACAGCA GCAATGAGCT GGGCAAGCGC CAGCCCAATG GAGTTGGCAT TGTGCCGGAA 
GACGTAAACA TCATTGTAAA CCGATTCAAG CAGGAGAAGC GGTCGCCGGA CAGCTCGTTG 
AGCGAGAATG GCTCGGTGCG CGGACGGCGT GGTATTGAGG TGCTGGTTGG CGGCAGCGGA 
GGATCCGACG TGGACAGCAT TGGCACAAAC ACTAGTCAGG AAAGCCGGCA CGAAACGGAT 
CACAACAACA AGCAGCAGTA TCCAGCGGCC GCACTGATGC CTCCACCACC TCCCTCGTTG 
ACGAATAACC ACAATCACGA GACCATTGAT GAGGAAGAGG AGGTGGTGAT CCGACCCAAG 
GCTAGGGTTC CGGCCGTAGT TAAGTCTGCA AACGCTCAAG GTCTCACCAA GGAAGAGATT 
GAGCTGCGCA ACCTGCGCAA GAAGACACGC AAGCGCACCC GCAAATTCGA GATCGATGGC 
GTGCAAATGA CCACCACCAC AAGCCGAGTA ATCTACGGGG ACGATGAGAA CGGACGAATC 
TACGATGATC ACGACTTCCG TAAGCAGGAG CTGCGTGAAC TAAAGCTGCT GCAGAAGCAG 
GAGAAGAAGC AGCAGACAGA ACTGCACTTA AAGGAGCAGC AGGCCAAGGA ACAGCAGGAT 
CGTCGCTTTG AGCAGGAGCG CTCCTCGCTG GAAAAGACCT ACGAGGCGGA CATGGATATG 
TTGGCGCGTC AGCACAAGCA GCTGGTGGAA AAGACAGAGC AGACGCAGGA GAACGAGCTG 
CGCTCCTCGT CTAAACGCAT TCGCTCCGAG CAGGAGCAAG AGTTGAAGAT CTTCCGAGAG 
AATCTCAAGC AGGAGATCCG ACTGCTCAAG CAGGAAGTTG ACCTGTTTCC CAAGGACAAG 
CGCAAGGATG AGTTTAAGCA GCGGCGCTCA GCCATGGAAC TGGACCACGA AGAGAAGGAA 
CGCGCCTTCC TGGATTCCTT AAAGGAGAGG CATGAGCTTC TACTAAGGCG ATTAAGCGAG 
AAGCATCGAG ACCACCTGGC CACTATCAAC CGCAACTTCT TGCAGCAGAA ACAGAATGCC 
ATGAGGACGC GGGAGGCCCT TCTCTGGGAA CTAGAGGAGA AGCAGCTTCA CGAACGACAT 
CAGTTGTCCA AGCGGCATGT AAAGGAACTA TGTTTCATGC AGCGACATCA GATGATAATC 
CGGCACGAGA AGGAGCTGGA CCAGGTGAAG CGCATGTTGC AGCGTAAGGA GGAGGACATG 
GTCAAGAAGC AGACGATGGA AAAGCGGGCT CTGCCGAAGC GTATCCGTGC AGAGCGCAAA 
GCGCGAGATC TTATGTTCCG TGAATCCCTG CGAATTTCCA CGAACCTAGA TCCTGAAATC 
GAACGCGATC GCTTGAAGAA GTTCCAGGAG CAAGAGAAGA AGCGGTATAT GCAGGAGGAG 
CGACGCTTCG AAGTCAAGCA TCAGAAGCAA TTGGAAGAGC TACGCGCTAC TCGCGAAAGC 
GCCATAAAAG AGCTGGAGCA ACTTCAGAAC GAAAAGCGAA GGGCTCTGGT GGAGCACGAA 
CACTCCAAGC TTTCCGAGAT CGATGAGCGA CTGAAGGGGG AGCTGCGCGA GTGGCGCGAA 
CAGTTGGTGC CCCGTAAACA GCAACTACAG CAACGGCTGC AACAAGAGCG CCTGGAGGAG 
ACCTTCGCCC AGCAGCTGGA CGAGATGGAG ACCCTGTACG GCGGCGCCCT GATTGTGTCC 
ATGCCCTCGG ACACGCTGCA GCGAGATCAC TTCACCGGCT CGACGCGCAG CAGCCTCAGC 
TCGTATTCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029447.1
CDS	412..4509
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEKLKRQVKRRSLYQREPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQQLQQRLQQERLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	XM_016181227.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant B (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181227.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGAAGCT TAAACGCCAA GTGAAGCGGC GCTCGCTCTA TCAGAGGGAA 
CCCCGCAACT CGGCGCTCCA GCTCGACCTG GACGATGACT CTGCTTCGCT GCAGAGCCAA 
GACATTGACA AACTTCCAGG TACACCTACA TCCATTTTGC GGGATGCCAA AGAGCAGTCC 
CAACCAAGTA GCAGTTTACC AATCGCAGCA GCGGCAACTG CAGCAGCAGC AGCTACAACC 
ACAACTAAAG CAACCACTCC GGACAGACCA AACCACACAA AGGATGACAA TGCTGAAGCG 
GCAGCCCAGC AGCCGCCGCA TACCAAAGTG CCTGCTCCAG CGCCGCCGTC GTCCCAACAA 
ACGCCACCAC CGCAAGTTCA GCAGCCACCT ACACCACCAG CCCAACCCAC AGCGGCTGTG 
CTGCAAAAGA AACCCGAAGA TGTGGCAGCT GTTGCAGAAA CATCTGAGAA AACCGAATCC 
GACAAGAAGC ACTTTGTCAA AAAGGGTAAG GCCCCGCCCC CGCCGTCCCC ATTGGGTCTT 
GCAAATGCCA AGCCGGCTGC CAGCGACTCC CAAACGTCTC CCAAAAAACT GGCCACTCCC 
GAGCCCACTT CTCCAGTCAC CACGGCTATT GAGGTGGCCA TTGGCCAAGA GGCGATGGAG 
CCGAAACCGC AGCCGCCATC ACCCACAGCC TCCTCCATTG TGTCTGTGCA ATCGGTGGCC 
TCTTCCAGCT CCTCGGGCAG TGTTTCAAAT GCTGTTCTCA GCTCGAGCAC TTCCCTTATT 
ACCATCAACA GCGATCCACC AACACCGCAT CATCACCAAC CGCTGCCACC TCAACCGCAG 
CACTTGGTTT TGCCAAACAG CTTGGAGTCG GTGAGTCAGA TAACAGTCGT GACCAGCACC 
CATCCGCCGG TGATCATCGA CAACTCGGTG GTGCCACCGC AAAACGAGGT GATCATCGTT 
TCCAATGATA TGAACAAGAG CACTCACCTG CATGAATCCT CAACGGACGA TGATTTTCCA 
TCGCTGGACG ACAGTTTGGG TGATGCCACC ACGCCGCCCC ACAAGCAATC CTCAATGATA 
TTGGCTGTTA ATGAGCCAGC AGGTGTGGTT CCCGCTCCTC CGTCGCAACC GCAAACGTCT 
TCCACTGTTC ACGCACGCAA ACTGGATGAA AGTGAGGTGT TAATAGTCAG TCCGTCCTAC 
GCGGATGATG ACTCGGCCTA TAACACGGCG TCTGGCAGTC ATAGCCACGA TCACAGTGAC 
CATCTGATGG ACACGAGCCA CGTCTCCGTG GTCACCGTGG GCGATGAGGG AGTTAAGGTG 
AAGGACAGCA GCAATGAGCT GGGCAAGCGC CAGCCCAATG GAGTTGGCAT TGTGCCGGAA 
GACGTAAACA TCATTGTAAA CCGATTCAAG CAGGAGAAGC GGTCGCCGGA CAGCTCGTTG 
AGCGAGAATG GCTCGGTGCG CGGACGGCGT GGTATTGAGG TGCTGGTTGG CGGCAGCGGA 
GGATCCGACG TGGACAGCAT TGGCACAAAC ACTAGTCAGG AAAGCCGGCA CGAAACGGAT 
CACAACAACA AGCAGCAGTA TCCAGCGGCC GCACTGATGC CTCCACCACC TCCCTCGTTG 
ACGAATAACC ACAATCACGA GACCATTGAT GAGGAAGAGG AGGTGGTGAT CCGACCCAAG 
GCTAGGGTTC CGGCCGTAGT TAAGTCTGCA AACGCTCAAG GTCTCACCAA GGAAGAGATT 
GAGCTGCGCA ACCTGCGCAA GAAGACACGC AAGCGCACCC GCAAATTCGA GATCGATGGC 
GTGCAAATGA CCACCACCAC AAGCCGAGTA ATCTACGGGG ACGATGAGAA CGGACGAATC 
TACGATGATC ACGACTTCCG TAAGCAGGAG CTGCGTGAAC TAAAGCTGCT GCAGAAGCAG 
GAGAAGAAGC AGCAGACAGA ACTGCACTTA AAGGAGCAGC AGGCCAAGGA ACAGCAGGAT 
CGTCGCTTTG AGCAGGAGCG CTCCTCGCTG GAAAAGACCT ACGAGGCGGA CATGGATATG 
TTGGCGCGTC AGCACAAGCA GCTGGTGGAA AAGACAGAGC AGACGCAGGA GAACGAGCTG 
CGCTCCTCGT CTAAACGCAT TCGCTCCGAG CAGGAGCAAG AGTTGAAGAT CTTCCGAGAG 
AATCTCAAGC AGGAGATCCG ACTGCTCAAG CAGGAAGTTG ACCTGTTTCC CAAGGACAAG 
CGCAAGGATG AGTTTAAGCA GCGGCGCTCA GCCATGGAAC TGGACCACGA AGAGAAGGAA 
CGCGCCTTCC TGGATTCCTT AAAGGAGAGG CATGAGCTTC TACTAAGGCG ATTAAGCGAG 
AAGCATCGAG ACCACCTGGC CACTATCAAC CGCAACTTCT TGCAGCAGAA ACAGAATGCC 
ATGAGGACGC GGGAGGCCCT TCTCTGGGAA CTAGAGGAGA AGCAGCTTCA CGAACGACAT 
CAGTTGTCCA AGCGGCATGT AAAGGAACTA TGTTTCATGC AGCGACATCA GATGATAATC 
CGGCACGAGA AGGAGCTGGA CCAGGTGAAG CGCATGTTGC AGCGTAAGGA GGAGGACATG 
GTCAAGAAGC AGACGATGGA AAAGCGGGCT CTGCCGAAGC GTATCCGTGC AGAGCGCAAA 
GCGCGAGATC TTATGTTCCG TGAATCCCTG CGAATTTCCA CGAACCTAGA TCCTGAAATC 
GAACGCGATC GCTTGAAGAA GTTCCAGGAG CAAGAGAAGA AGCGGTATAT GCAGGAGGAG 
CGACGCTTCG AAGTCAAGCA TCAGAAGCAA TTGGAAGAGC TACGCGCTAC TCGCGAAAGC 
GCCATAAAAG AGCTGGAGCA ACTTCAGAAC GAAAAGCGAA GGGCTCTGGT GGAGCACGAA 
CACTCCAAGC TTTCCGAGAT CGATGAGCGA CTGAAGGGGG AGCTGCGCGA GTGGCGCGAA 
CAGTTGGTGC CCCGTAAACA GCAACTACAG CAACGGCTGC AACAAGAGCG CCTGGAGGAG 
ACCTTCGCCC AGCAGCTGGA CGAGATGGAG ACCCTGTACG GCGGCGCCCT GATTGTGTCC 
ATGCCCTCGG ACACGCTGCA GCGAGATCAC TTCACCGGCT CGACGCGCAG CAGCCTCAGC 
TCGTATTCCG AGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181231.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029451.1 Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD24961), mRNA.

Price & Availability↑

CloneID	ODg22159
Clone ID Related Accession (Same CDS sequence)	XM_016181231.1 , XM_016181230.1
Accession Version	XM_016181231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform F
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC GCCTGGAGGA GACCTTCGCC 
CAGCAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029451.1
CDS	990..5018
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	XM_016181231.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant F (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181231.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC GCCTGGAGGA GACCTTCGCC 
CAGCAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181230.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029450.1 Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD24961), mRNA.

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CloneID	ODg22159
Clone ID Related Accession (Same CDS sequence)	XM_016181231.1 , XM_016181230.1
Accession Version	XM_016181230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC GCCTGGAGGA GACCTTCGCC 
CAGCAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029450.1
CDS	412..4440
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	XM_016181230.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant E (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181230.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC GCCTGGAGGA GACCTTCGCC 
CAGCAGCTGG ACGAGATGGA GACCCTGTAC GGCGGCGCCC TGATTGTGTC CATGCCCTCG 
GACACGCTGC AGCGAGATCA CTTCACCGGC TCGACGCGCA GCAGCCTCAG CTCGTATTCC 
GAGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181232.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029452.1 Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD24961), mRNA.

Price & Availability↑

CloneID	ODg22160
Clone ID Related Accession (Same CDS sequence)	XM_016181232.1
Accession Version	XM_016181232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4056bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform G
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC AACTACAGCA ACGGCTGCAA 
CAAGAGCGCC TGGAGGAGAC CTTCGCCCAG CAGCTGGACG AGATGGAGAC CCTGTACGGC 
GGCGCCCTGA TTGTGTCCAT GCCCTCGGAC ACGCTGCAGC GAGATCACTT CACCGGCTCG 
ACGCGCAGCA GCCTCAGCTC GTATTCCGAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029452.1
CDS	990..5045
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQQLQQRLQQERLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	XM_016181232.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant G (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181232.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGC AACTACAGCA ACGGCTGCAA 
CAAGAGCGCC TGGAGGAGAC CTTCGCCCAG CAGCTGGACG AGATGGAGAC CCTGTACGGC 
GGCGCCCTGA TTGTGTCCAT GCCCTCGGAC ACGCTGCAGC GAGATCACTT CACCGGCTCG 
ACGCGCAGCA GCCTCAGCTC GTATTCCGAG GGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181228.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029448.1 Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD24961), mRNA.

Price & Availability↑

CloneID	ODg22157
Clone ID Related Accession (Same CDS sequence)	XM_016181228.1 , XM_016181229.1
Accession Version	XM_016181228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform C
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029448.1
CDS	412..5319
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVANREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPSTPIMHKQQRSQMISDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPAMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVASADSAASSEDIYYDLYAAQYRLPRVNQRQHSCEEDSSAI

Target ORF information:

RefSeq Version	XM_016181228.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant C (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181228.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181229.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029449.1 Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD24961), mRNA.

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CloneID	ODg22157
Clone ID Related Accession (Same CDS sequence)	XM_016181228.1 , XM_016181229.1
Accession Version	XM_016181229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform D
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029449.1
CDS	991..5898
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVANREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPSTPIMHKQQRSQMISDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPAMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVASADSAASSEDIYYDLYAAQYRLPRVNQRQHSCEEDSSAI

Target ORF information:

RefSeq Version	XM_016181229.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant D (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181229.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
GTCAATCAGC GGCAGCACAG CTGCGAGGAG GACTCGTCGG CCATCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dsim\GD24961 ( XM_016181226.1 ) cDNA ORF clone, Drosophila simulans -> XP_016029446.1 Drosophila simulans uncharacterized protein, transcript variant A (Dsim\GD24961), mRNA.

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CloneID	ODg22158
Clone ID Related Accession (Same CDS sequence)	XM_016181226.1
Accession Version	XM_016181226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-03
Organism	Drosophila simulans
Product	uncharacterized protein, isoform A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NT_479534). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 2.01 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGCGCTTCC 
CAGCCATTCG ATATGACCCA GGGCCCTGCA ACTGGAGCTC GCCGCCTGGA GGAGACCTTC 
GCCCAGCAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016029446.1
CDS	412..5523
Translation	MSFITNLKKVFHLGGGEAKKKRLYNNIKMDTDPAEFWEMVGELGDGAFGKVYKAQHKEQKRFAAAKMCQLEDEENLSDHMVEIDILSEIKHPNIVELYEAFSIDDKLWMLIEYCDGGALDSIMVELEKPLTEPQIAYVCKHMTEGLTFLHRNKVIHRDLKAGNVLLTMEGGVKLADFGVSAKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDHKVDIWSLGITLIELAQMEPPNSEMSPMRVLLKIQKSEPPKLEQPSRWSKEFNDFLKKSLVKDPQVRPTTDVLMQHAFINRNLDAKPIKDLLLEYKAEVVEEVVDDETEEPRNSALQLDLDDDSASLQSQDIDKLPGTPTSILRDAKEQSQPSSSLPIAAAATAAAAATTTTKATTPDRPNHTKDDNAEAAAQQPPHTKVPAPAPPSSQQTPPPQVQQPPTPPAQPTAAVLQKKPEDVAAVAETSEKTESDKKHFVKKGKAPPPPSPLGLANAKPAASDSQTSPKKLATPEPTSPVTTAIEVAIGQEAMEPKPQPPSPTASSIVSVQSVASSSSSGSVSNAVLSSSTSLITINSDPPTPHHHQPLPPQPQHLVLPNSLESVSQITVVTSTHPPVIIDNSVVPPQNEVIIVSNDMNKSTHLHESSTDDDFPSLDDSLGDATTPPHKQSSMILAVNEPAGVVPAPPSQPQTSSTVHARKLDESEVLIVSPSYADDDSAYNTASGSHSHDHSDHLMDTSHVSVVTVGDEGVKVKDSSNELGKRQPNGVGIVPEDVNIIVNRFKQEKRSPDSSLSENGSVRGRRGIEVLVGGSGGSDVDSIGTNTSQESRHETDHNNKQQYPAAALMPPPPPSLTNNHNHETIDEEEEVVIRPKARVPAVVKSANAQGLTKEEIELRNLRKKTRKRTRKFEIDGVQMTTTTSRVIYGDDENGRIYDDHDFRKQELRELKLLQKQEKKQQTELHLKEQQAKEQQDRRFEQERSSLEKTYEADMDMLARQHKQLVEKTEQTQENELRSSSKRIRSEQEQELKIFRENLKQEIRLLKQEVDLFPKDKRKDEFKQRRSAMELDHEEKERAFLDSLKERHELLLRRLSEKHRDHLATINRNFLQQKQNAMRTREALLWELEEKQLHERHQLSKRHVKELCFMQRHQMIIRHEKELDQVKRMLQRKEEDMVKKQTMEKRALPKRIRAERKARDLMFRESLRISTNLDPEIERDRLKKFQEQEKKRYMQEERRFEVKHQKQLEELRATRESAIKELEQLQNEKRRALVEHEHSKLSEIDERLKGELREWREQLVPRKQELNRQIKLAIDQHEKRFGLVANREEFEDQEVKLPAHLRNIYNERSSRILPRNTFIDPQNMPRSRSSLLMMGGGGNRLSNFRGSAPDLSRSVPSTPIMHKQQRSQMISDQVLEEDEEQMLSEQMKRASVATLPAQNQLRLITQLPANELVKVVTSTPPLNSAEDVNSKPAMSTFRGSTPPKTPDDLGLVTNRFSRLDTKAAAEASGVELRIHEGPVLTAHTQRLLHTTSFAIKRPSLASRSSGRSSLSSAQSMYNINELSTRFTSDADLIFDSGRKAAALLDEVQPQLRSSVKPGHSRANGSVASADSAASSEDIYYDLYAAQYRLPRQLQQRLQQEVSPLIYPQCASQPFDMTQGPATGARRLEETFAQQLDEMETLYGGALIVSMPSDTLQRDHFTGSTRSSLSSYSEG

Target ORF information:

RefSeq Version	XM_016181226.1
Organism	Drosophila simulans
Definition	Drosophila simulans uncharacterized protein, transcript variant A (Dsim\GD24961), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016181226.1

ORF Insert Sequence:

ATGTCGTTTA TCACCAACCT GAAGAAGGTC TTCCACCTCG GCGGGGGTGA GGCCAAGAAG 
AAGCGCCTGT ACAACAACAT CAAGATGGAC ACGGATCCGG CGGAGTTCTG GGAAATGGTC 
GGCGAACTCG GCGACGGAGC CTTCGGCAAG GTGTACAAGG CGCAGCACAA GGAGCAGAAG 
CGGTTTGCTG CCGCTAAAAT GTGCCAGCTG GAGGACGAGG AGAACCTTAG CGATCACATG 
GTCGAAATCG ACATTCTGTC CGAGATCAAG CACCCAAATA TAGTGGAGTT GTATGAGGCC 
TTTTCCATTG ATGATAAGCT GTGGATGCTC ATCGAGTACT GCGACGGAGG GGCCCTGGAT 
AGCATTATGG TGGAGCTGGA AAAGCCCCTG ACTGAGCCGC AGATTGCCTA TGTTTGCAAG 
CACATGACCG AGGGTCTCAC CTTCCTGCAT CGCAACAAAG TTATCCATCG TGATTTGAAG 
GCAGGAAACG TGCTTCTCAC AATGGAAGGT GGCGTTAAGT TGGCGGACTT TGGCGTTTCG 
GCCAAAAACA AGCACACCAT GCAGAAGCAC GACACCTTCA TTGGCACTCC CTACTGGATG 
GCGCCGGAGC TGGTGCTGTG CGAAACGTTC CGGGACAACC CGTACGATCA CAAAGTGGAC 
ATCTGGTCAC TGGGCATCAC GCTAATCGAG CTGGCGCAGA TGGAGCCGCC CAACAGCGAG 
ATGTCGCCCA TGCGAGTGCT CCTAAAAATT CAAAAGAGCG AGCCGCCCAA GCTGGAGCAG 
CCTTCTCGAT GGAGCAAGGA GTTTAACGAC TTCCTGAAGA AGTCCTTAGT CAAGGATCCT 
CAGGTGAGGC CCACGACGGA TGTGCTGATG CAGCACGCTT TCATCAACAG AAATCTGGAT 
GCCAAGCCTA TTAAGGATCT GCTGCTCGAG TACAAGGCCG AGGTCGTGGA AGAGGTGGTG 
GATGATGAGA CCGAGGAACC CCGCAACTCG GCGCTCCAGC TCGACCTGGA CGATGACTCT 
GCTTCGCTGC AGAGCCAAGA CATTGACAAA CTTCCAGGTA CACCTACATC CATTTTGCGG 
GATGCCAAAG AGCAGTCCCA ACCAAGTAGC AGTTTACCAA TCGCAGCAGC GGCAACTGCA 
GCAGCAGCAG CTACAACCAC AACTAAAGCA ACCACTCCGG ACAGACCAAA CCACACAAAG 
GATGACAATG CTGAAGCGGC AGCCCAGCAG CCGCCGCATA CCAAAGTGCC TGCTCCAGCG 
CCGCCGTCGT CCCAACAAAC GCCACCACCG CAAGTTCAGC AGCCACCTAC ACCACCAGCC 
CAACCCACAG CGGCTGTGCT GCAAAAGAAA CCCGAAGATG TGGCAGCTGT TGCAGAAACA 
TCTGAGAAAA CCGAATCCGA CAAGAAGCAC TTTGTCAAAA AGGGTAAGGC CCCGCCCCCG 
CCGTCCCCAT TGGGTCTTGC AAATGCCAAG CCGGCTGCCA GCGACTCCCA AACGTCTCCC 
AAAAAACTGG CCACTCCCGA GCCCACTTCT CCAGTCACCA CGGCTATTGA GGTGGCCATT 
GGCCAAGAGG CGATGGAGCC GAAACCGCAG CCGCCATCAC CCACAGCCTC CTCCATTGTG 
TCTGTGCAAT CGGTGGCCTC TTCCAGCTCC TCGGGCAGTG TTTCAAATGC TGTTCTCAGC 
TCGAGCACTT CCCTTATTAC CATCAACAGC GATCCACCAA CACCGCATCA TCACCAACCG 
CTGCCACCTC AACCGCAGCA CTTGGTTTTG CCAAACAGCT TGGAGTCGGT GAGTCAGATA 
ACAGTCGTGA CCAGCACCCA TCCGCCGGTG ATCATCGACA ACTCGGTGGT GCCACCGCAA 
AACGAGGTGA TCATCGTTTC CAATGATATG AACAAGAGCA CTCACCTGCA TGAATCCTCA 
ACGGACGATG ATTTTCCATC GCTGGACGAC AGTTTGGGTG ATGCCACCAC GCCGCCCCAC 
AAGCAATCCT CAATGATATT GGCTGTTAAT GAGCCAGCAG GTGTGGTTCC CGCTCCTCCG 
TCGCAACCGC AAACGTCTTC CACTGTTCAC GCACGCAAAC TGGATGAAAG TGAGGTGTTA 
ATAGTCAGTC CGTCCTACGC GGATGATGAC TCGGCCTATA ACACGGCGTC TGGCAGTCAT 
AGCCACGATC ACAGTGACCA TCTGATGGAC ACGAGCCACG TCTCCGTGGT CACCGTGGGC 
GATGAGGGAG TTAAGGTGAA GGACAGCAGC AATGAGCTGG GCAAGCGCCA GCCCAATGGA 
GTTGGCATTG TGCCGGAAGA CGTAAACATC ATTGTAAACC GATTCAAGCA GGAGAAGCGG 
TCGCCGGACA GCTCGTTGAG CGAGAATGGC TCGGTGCGCG GACGGCGTGG TATTGAGGTG 
CTGGTTGGCG GCAGCGGAGG ATCCGACGTG GACAGCATTG GCACAAACAC TAGTCAGGAA 
AGCCGGCACG AAACGGATCA CAACAACAAG CAGCAGTATC CAGCGGCCGC ACTGATGCCT 
CCACCACCTC CCTCGTTGAC GAATAACCAC AATCACGAGA CCATTGATGA GGAAGAGGAG 
GTGGTGATCC GACCCAAGGC TAGGGTTCCG GCCGTAGTTA AGTCTGCAAA CGCTCAAGGT 
CTCACCAAGG AAGAGATTGA GCTGCGCAAC CTGCGCAAGA AGACACGCAA GCGCACCCGC 
AAATTCGAGA TCGATGGCGT GCAAATGACC ACCACCACAA GCCGAGTAAT CTACGGGGAC 
GATGAGAACG GACGAATCTA CGATGATCAC GACTTCCGTA AGCAGGAGCT GCGTGAACTA 
AAGCTGCTGC AGAAGCAGGA GAAGAAGCAG CAGACAGAAC TGCACTTAAA GGAGCAGCAG 
GCCAAGGAAC AGCAGGATCG TCGCTTTGAG CAGGAGCGCT CCTCGCTGGA AAAGACCTAC 
GAGGCGGACA TGGATATGTT GGCGCGTCAG CACAAGCAGC TGGTGGAAAA GACAGAGCAG 
ACGCAGGAGA ACGAGCTGCG CTCCTCGTCT AAACGCATTC GCTCCGAGCA GGAGCAAGAG 
TTGAAGATCT TCCGAGAGAA TCTCAAGCAG GAGATCCGAC TGCTCAAGCA GGAAGTTGAC 
CTGTTTCCCA AGGACAAGCG CAAGGATGAG TTTAAGCAGC GGCGCTCAGC CATGGAACTG 
GACCACGAAG AGAAGGAACG CGCCTTCCTG GATTCCTTAA AGGAGAGGCA TGAGCTTCTA 
CTAAGGCGAT TAAGCGAGAA GCATCGAGAC CACCTGGCCA CTATCAACCG CAACTTCTTG 
CAGCAGAAAC AGAATGCCAT GAGGACGCGG GAGGCCCTTC TCTGGGAACT AGAGGAGAAG 
CAGCTTCACG AACGACATCA GTTGTCCAAG CGGCATGTAA AGGAACTATG TTTCATGCAG 
CGACATCAGA TGATAATCCG GCACGAGAAG GAGCTGGACC AGGTGAAGCG CATGTTGCAG 
CGTAAGGAGG AGGACATGGT CAAGAAGCAG ACGATGGAAA AGCGGGCTCT GCCGAAGCGT 
ATCCGTGCAG AGCGCAAAGC GCGAGATCTT ATGTTCCGTG AATCCCTGCG AATTTCCACG 
AACCTAGATC CTGAAATCGA ACGCGATCGC TTGAAGAAGT TCCAGGAGCA AGAGAAGAAG 
CGGTATATGC AGGAGGAGCG ACGCTTCGAA GTCAAGCATC AGAAGCAATT GGAAGAGCTA 
CGCGCTACTC GCGAAAGCGC CATAAAAGAG CTGGAGCAAC TTCAGAACGA AAAGCGAAGG 
GCTCTGGTGG AGCACGAACA CTCCAAGCTT TCCGAGATCG ATGAGCGACT GAAGGGGGAG 
CTGCGCGAGT GGCGCGAACA GTTGGTGCCC CGTAAACAGG AGCTGAACCG TCAGATCAAG 
CTGGCCATTG ATCAGCACGA AAAGCGATTC GGCCTCGTGG CCAACCGCGA GGAGTTCGAG 
GATCAGGAGG TCAAGCTGCC CGCCCACCTG CGCAACATCT ACAACGAGCG ATCCTCACGC 
ATTCTGCCCC GCAACACCTT TATCGACCCT CAAAACATGC CGCGCTCTCG CTCCTCGCTC 
CTAATGATGG GGGGTGGTGG CAACCGACTG TCCAACTTCC GCGGATCAGC TCCAGATCTT 
AGCCGCAGTG TGCCCAGCAC TCCCATTATG CACAAACAAC AGAGATCGCA GATGATAAGC 
GATCAGGTGC TAGAAGAGGA CGAGGAGCAG ATGCTGAGCG AGCAGATGAA GCGGGCCAGC 
GTCGCCACGC TTCCGGCGCA AAACCAACTT CGTCTTATTA CTCAGCTTCC GGCAAACGAG 
CTCGTGAAGG TGGTGACCTC CACGCCACCA CTGAACAGCG CGGAAGATGT GAACTCCAAG 
CCAGCCATGA GCACATTCAG AGGCAGTACG CCGCCCAAAA CACCAGATGA TCTGGGCTTG 
GTCACCAATC GATTCAGCCG CCTTGACACC AAGGCTGCGG CGGAGGCCAG CGGCGTGGAG 
CTGCGCATTC ACGAGGGGCC AGTGCTAACT GCGCACACTC AGCGGCTGCT GCACACCACC 
TCCTTTGCTA TCAAGCGACC CTCGCTGGCC AGCCGGAGCA GCGGCCGATC CTCCCTGAGC 
AGCGCGCAGT CGATGTACAA CATAAACGAG CTGTCCACGC GGTTCACCAG TGATGCGGAC 
CTGATATTTG ACTCCGGACG CAAGGCAGCC GCCCTGCTAG ACGAAGTGCA GCCGCAGCTA 
CGGTCCTCCG TTAAGCCAGG ACACAGTCGT GCCAACGGAA GCGTCGCCTC GGCCGACTCC 
GCCGCCTCTT CCGAGGATAT CTACTACGAC CTGTATGCGG CGCAGTACAG ACTGCCGAGA 
CAACTACAGC AACGGCTGCA ACAAGAGGTT AGTCCTTTGA TCTATCCCCA GTGCGCTTCC 
CAGCCATTCG ATATGACCCA GGGCCCTGCA ACTGGAGCTC GCCGCCTGGA GGAGACCTTC 
GCCCAGCAGC TGGACGAGAT GGAGACCCTG TACGGCGGCG CCCTGATTGT GTCCATGCCC 
TCGGACACGC TGCAGCGAGA TCACTTCACC GGCTCGACGC GCAGCAGCCT CAGCTCGTAT 
TCCGAGGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A second-generation assembly of the Drosophila simulans genome provides new insights into patterns of lineage-specific divergence.
Genome research23(1)89-98(2013 Jan)
Hu TT,Eisen MB,Thornton KR,Andolfatto P

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Dsim\GD24961 cDNA ORF clone, Drosophila simulans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム