Pif1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pif1A
Entrez Gene ID	8674049
Full Name	PFTAIRE-interacting factor 1A
Synonyms	BcDNA:GH12504,CG11773,CG11774,CG11776,CG32940,CG33719,CG33719/CG33720,CG42599,CG9808,DM22,Dmel\CG42599,Dmel_CG11776,Dmel_CG33719,IP06413p,PIF-1A,Pif1
General protein information	Preferred Names PFTAIRE-interacting factor 1A Names CG42599 gene product from transcript CG42599-RH CG42599-PA CG42599-PG CG42599-PH CG42599-PI CG42599-PJ Pif1A-PA Pif1A-PG Pif1A-PH Pif1A-PI Pif1A-PJ
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85B1-85B1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001031987.1	NP_001027158.1	PFTAIRE-interacting factor 1A, isoform A
NM_001202266.2	NP_001189195.2	PFTAIRE-interacting factor 1A, isoform H
NM_001316487.1	NP_001303416.1	PFTAIRE-interacting factor 1A, isoform J
NM_001170085.2	NP_001163556.2	PFTAIRE-interacting factor 1A, isoform G
NM_001031986.2	NP_001027157.2	PFTAIRE-interacting factor 1A, isoform I

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pif1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pif1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa07426	NM_001031987.1 Latest version!	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa07427	NM_001031986.2 Latest version!	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant I, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OFa07701	NM_001202266.2 Latest version!	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant H, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa07701	NM_001316487.1 Latest version!	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant J, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OFa07702	NM_001170085.2 Latest version!	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant G, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pif1A ( NM_001031987.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027158.1 Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa07426
Clone ID Related Accession (Same CDS sequence)	NM_001031987.1
Accession Version	NM_001031987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PFTAIRE-interacting factor 1A, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGATTACT ACTATAACCG AAGGGATGTC AAGATAACCC AACTCAAGAA CATTGAGCTA 
ATCAACAATA CGAAAAATGC ACATAAATCA GCGCTCGAGA TGCGCACAAC AATGAAGAGC 
AGTCAGCCGA ATGGATTGGA TGATGCTCCT ACACGGATGA GGGCCCCCGA CGAGATGAGG 
GCCACCACTT GGATGACGAC GCTGGTAGGC AGTAACTGCC ATAGCCACAG CCACAGCCAT 
CCGGCAGTAG CCAAGTCGAA GGATCCCAAT TCGGACACGG ACTCGGCCTT GTCCTCGGCC 
CCGCCCTCCA TCAGCCCGCA GCCGCCATCC AGCGGATCCG ACAGCGGCGT GATTTGGCAA 
ACACTGCAGG ACCTGGACAA GTTGCAAAAG GAAGCGGAAA TGTACCAAAA GGAGAACGAA 
CGCCTGCAGA CGGAGGTGCA GCTGATGAAA CAGGAGCTGG ATGCCGCGGA AAAGGCGGCC 
ATAAGCAGGG CCAAGAAGCA GGCCCAGATC GGGGAGCTGA TGCAGCGCAT CAAGGAGCTG 
GAAGAGATGC AGTCGAGCTT GGAGGACGAG GCCAGCGAAC TGAGGGAGCA GAATGAACTC 
CTTGAGTTTC GCATACTCGA GCTGGAAGAT GATAGCGATA AGATGGAGAG CACCTGCGAG 
GGCCACTGCC AGAGTCTGCA GGATCTGCTG GAGCAGTCTG CCCAGCGGCT GGAGGATCGG 
GACAAGCGCT GCTTGCAACA ATTGCTGCAG TGCGTCCAGC AACTGGATCT GGATACCATG 
ATGCCAGGCG ATCAGAACCG GACGGAAACT CCCAAAAGGC ACAATTCAAC CCACAGTCAC 
AGTCACAGTC ACAGCCACAG TCAAAGTCAC AACCACAGGA TCGTAGCCAC AGTTCAGCCG 
TATAGCAACT GTGCCACGCC CACAGCCCCG CCCACGCCAA GCAAGAGGCA CTGCAACCAG 
GTGAGCTCGT GGCAGAGCAG CAGCCTCAGC GAGAGCGGAG TGTTCGTAGA GTGCGATCTG 
CTTTCGAATC CCAATCCCAT TGGCCACCCG CATCTGCAGT TCTATCAGGA GAGATTGGAG 
CAGTTGGAGG GCAAGCTGCT GATCTACGAG AGCAGCGGCG AGACCCAGGC ACGGCACTTG 
GCCCAGAGAC TGCAGCGGGA ACTGCAGCTG GAGAAGGATC TCAAAGAGCT TGCGGACCGA 
GTGGAATTGC TAATCGAGCA AAACGCCCAG TTGGAGGAGG CCAAGTGCGA GTTTGAGGAG 
GCCGAGAACG ATACACGACT CCATTTGCAG CGTAACGAGG TGGAACTTGA AATTCTTCGC 
CAGCGCAACG TGGAGTTGGA ATTCGGCAAG GAAGCACTAG GCGCCAAGTA TCAGGATTGC 
AGGGCGGAGG TACTAATCCT TCGAGAGGAT CTAGCGGCAG CTGAAACCCA ACTGGAGCAT 
CTGCAGGCCG AAAGGAAGCA AGCCAGAAAG GAGCTTCAGG ATCTGAGAAG ATCGCTTCCA 
TTACTCCTAA TCTTCCGCTT GTTATCACTA GCCAAAATGG GCAACGAGGA ATCCTCCCCG 
GGAGGCAGCC CCCGCCTCTC CTCGGGCTAC ACTTCTTCTA TACACCAGGA TCGAGATTTC 
AGTGCCAAGA TCCAAACAAC GGAGTTTGAC CTCGGTCAAG CGGGATTTAC CGATTCCGGC 
GAGGAACGGG AGATCGTTTA CCTAAAGGAA GAGGTCAAGA GTCTGCGTAG CCAACTGAAA 
GAGCTAAATG CCCGGCACTA CGAAGCCATG GAAACAGCAG ACTCACACTG GGTAGATCTG 
GAGCAGCAGT ACAAGGAGCG CGAGGAGGCC CAACAGGCCA AGGAGGCCAG TCTCAAGCAG 
AAGATAGCCC AACTGCAAGA TTGCCTCCGG GAGGATTCCC GAGCGGCGAC CGAAAAGATC 
CAGCAGCTGG AGGAGGGGGA ACTGTCATTG AAGTCCTGCC TGGTCCGCAT GACCAAGGAG 
CATCGCGACC TGCTCACCGA GAATCGCACT CTGCAGTGCA GCTTGGAGAG TCTAATGGCC 
AAAATGGAAA AGGAGGCGGA ACACAAGATG CCCCTAACAG AGGCTCTAGA AAACGAGAGG 
CGCAAAACCC AGGCACTGAT GGATGACCTT ATCTTTGCCA AGAAGGTGCA GCAGAACACC 
GAGGATCAGT TGAGGCAGGA GACGGATGCT TTGCGCACCC AGATCTTCAA CATCAAAAAG 
GATTACCTGC ACATCGAGGT CACAAATGGC GAGCTGAAAG AGGAGGTGGG CACGCTGGAG 
AACAAAATCC GCCAGATGGA GAACCAAATG AGGGATTCGG AGGAGCGGGC GCGCTGCCTG 
GAGGACGAGC TGCGAACCAA GGACGAACAA TGCCAGCTCT TGGAACGCAA ACTGGGCGTT 
ATGCCCGAGG GCTACAGCCT GGCCGATGAA CTCCATGATA GTCCTGCGAA GCGGGCCAAG 
AAGGATGAGG TACCGAGCCT CCAGGCTGCC AGCCAGGGAC TGGGCGTGGC CCTACTCGAT 
CTCGAGGGTC GTGATTTTGG TCTACCACTG CACAAGGACT TCCAAGCAAT TGCCTGCAGT 
GTAAAGCAAC TGGCGGATAC CCTTCTCTCA CAGAGTCCGT CCGAGAATGC CCGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027158.1
CDS	233..2872
Translation	MDYYYNRRDVKITQLKNIELINNTKNAHKSALEMRTTMKSSQPNGLDDAPTRMRAPDEMRATTWMTTLVGSNCHSHSHSHPAVAKSKDPNSDTDSALSSAPPSISPQPPSSGSDSGVIWQTLQDLDKLQKEAEMYQKENERLQTEVQLMKQELDAAEKAAISRAKKQAQIGELMQRIKELEEMQSSLEDEASELREQNELLEFRILELEDDSDKMESTCEGHCQSLQDLLEQSAQRLEDRDKRCLQQLLQCVQQLDLDTMMPGDQNRTETPKRHNSTHSHSHSHSHSQSHNHRIVATVQPYSNCATPTAPPTPSKRHCNQVSSWQSSSLSESGVFVECDLLSNPNPIGHPHLQFYQERLEQLEGKLLIYESSGETQARHLAQRLQRELQLEKDLKELADRVELLIEQNAQLEEAKCEFEEAENDTRLHLQRNEVELEILRQRNVELEFGKEALGAKYQDCRAEVLILREDLAAAETQLEHLQAERKQARKELQDLRRSLPLLLIFRLLSLAKMGNEESSPGGSPRLSSGYTSSIHQDRDFSAKIQTTEFDLGQAGFTDSGEEREIVYLKEEVKSLRSQLKELNARHYEAMETADSHWVDLEQQYKEREEAQQAKEASLKQKIAQLQDCLREDSRAATEKIQQLEEGELSLKSCLVRMTKEHRDLLTENRTLQCSLESLMAKMEKEAEHKMPLTEALENERRKTQALMDDLIFAKKVQQNTEDQLRQETDALRTQIFNIKKDYLHIEVTNGELKEEVGTLENKIRQMENQMRDSEERARCLEDELRTKDEQCQLLERKLGVMPEGYSLADELHDSPAKRAKKDEVPSLQAASQGLGVALLDLEGRDFGLPLHKDFQAIACSVKQLADTLLSQSPSENARL

Target ORF information:

RefSeq Version	NM_001031987.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031987.1

ORF Insert Sequence:

ATGGATTACT ACTATAACCG AAGGGATGTC AAGATAACCC AACTCAAGAA CATTGAGCTA 
ATCAACAATA CGAAAAATGC ACATAAATCA GCGCTCGAGA TGCGCACAAC AATGAAGAGC 
AGTCAGCCGA ATGGATTGGA TGATGCTCCT ACACGGATGA GGGCCCCCGA CGAGATGAGG 
GCCACCACTT GGATGACGAC GCTGGTAGGC AGTAACTGCC ATAGCCACAG CCACAGCCAT 
CCGGCAGTAG CCAAGTCGAA GGATCCCAAT TCGGACACGG ACTCGGCCTT GTCCTCGGCC 
CCGCCCTCCA TCAGCCCGCA GCCGCCATCC AGCGGATCCG ACAGCGGCGT GATTTGGCAA 
ACACTGCAGG ACCTGGACAA GTTGCAAAAG GAAGCGGAAA TGTACCAAAA GGAGAACGAA 
CGCCTGCAGA CGGAGGTGCA GCTGATGAAA CAGGAGCTGG ATGCCGCGGA AAAGGCGGCC 
ATAAGCAGGG CCAAGAAGCA GGCCCAGATC GGGGAGCTGA TGCAGCGCAT CAAGGAGCTG 
GAAGAGATGC AGTCGAGCTT GGAGGACGAG GCCAGCGAAC TGAGGGAGCA GAATGAACTC 
CTTGAGTTTC GCATACTCGA GCTGGAAGAT GATAGCGATA AGATGGAGAG CACCTGCGAG 
GGCCACTGCC AGAGTCTGCA GGATCTGCTG GAGCAGTCTG CCCAGCGGCT GGAGGATCGG 
GACAAGCGCT GCTTGCAACA ATTGCTGCAG TGCGTCCAGC AACTGGATCT GGATACCATG 
ATGCCAGGCG ATCAGAACCG GACGGAAACT CCCAAAAGGC ACAATTCAAC CCACAGTCAC 
AGTCACAGTC ACAGCCACAG TCAAAGTCAC AACCACAGGA TCGTAGCCAC AGTTCAGCCG 
TATAGCAACT GTGCCACGCC CACAGCCCCG CCCACGCCAA GCAAGAGGCA CTGCAACCAG 
GTGAGCTCGT GGCAGAGCAG CAGCCTCAGC GAGAGCGGAG TGTTCGTAGA GTGCGATCTG 
CTTTCGAATC CCAATCCCAT TGGCCACCCG CATCTGCAGT TCTATCAGGA GAGATTGGAG 
CAGTTGGAGG GCAAGCTGCT GATCTACGAG AGCAGCGGCG AGACCCAGGC ACGGCACTTG 
GCCCAGAGAC TGCAGCGGGA ACTGCAGCTG GAGAAGGATC TCAAAGAGCT TGCGGACCGA 
GTGGAATTGC TAATCGAGCA AAACGCCCAG TTGGAGGAGG CCAAGTGCGA GTTTGAGGAG 
GCCGAGAACG ATACACGACT CCATTTGCAG CGTAACGAGG TGGAACTTGA AATTCTTCGC 
CAGCGCAACG TGGAGTTGGA ATTCGGCAAG GAAGCACTAG GCGCCAAGTA TCAGGATTGC 
AGGGCGGAGG TACTAATCCT TCGAGAGGAT CTAGCGGCAG CTGAAACCCA ACTGGAGCAT 
CTGCAGGCCG AAAGGAAGCA AGCCAGAAAG GAGCTTCAGG ATCTGAGAAG ATCGCTTCCA 
TTACTCCTAA TCTTCCGCTT GTTATCACTA GCCAAAATGG GCAACGAGGA ATCCTCCCCG 
GGAGGCAGCC CCCGCCTCTC CTCGGGCTAC ACTTCTTCTA TACACCAGGA TCGAGATTTC 
AGTGCCAAGA TCCAAACAAC GGAGTTTGAC CTCGGTCAAG CGGGATTTAC CGATTCCGGC 
GAGGAACGGG AGATCGTTTA CCTAAAGGAA GAGGTCAAGA GTCTGCGTAG CCAACTGAAA 
GAGCTAAATG CCCGGCACTA CGAAGCCATG GAAACAGCAG ACTCACACTG GGTAGATCTG 
GAGCAGCAGT ACAAGGAGCG CGAGGAGGCC CAACAGGCCA AGGAGGCCAG TCTCAAGCAG 
AAGATAGCCC AACTGCAAGA TTGCCTCCGG GAGGATTCCC GAGCGGCGAC CGAAAAGATC 
CAGCAGCTGG AGGAGGGGGA ACTGTCATTG AAGTCCTGCC TGGTCCGCAT GACCAAGGAG 
CATCGCGACC TGCTCACCGA GAATCGCACT CTGCAGTGCA GCTTGGAGAG TCTAATGGCC 
AAAATGGAAA AGGAGGCGGA ACACAAGATG CCCCTAACAG AGGCTCTAGA AAACGAGAGG 
CGCAAAACCC AGGCACTGAT GGATGACCTT ATCTTTGCCA AGAAGGTGCA GCAGAACACC 
GAGGATCAGT TGAGGCAGGA GACGGATGCT TTGCGCACCC AGATCTTCAA CATCAAAAAG 
GATTACCTGC ACATCGAGGT CACAAATGGC GAGCTGAAAG AGGAGGTGGG CACGCTGGAG 
AACAAAATCC GCCAGATGGA GAACCAAATG AGGGATTCGG AGGAGCGGGC GCGCTGCCTG 
GAGGACGAGC TGCGAACCAA GGACGAACAA TGCCAGCTCT TGGAACGCAA ACTGGGCGTT 
ATGCCCGAGG GCTACAGCCT GGCCGATGAA CTCCATGATA GTCCTGCGAA GCGGGCCAAG 
AAGGATGAGG TACCGAGCCT CCAGGCTGCC AGCCAGGGAC TGGGCGTGGC CCTACTCGAT 
CTCGAGGGTC GTGATTTTGG TCTACCACTG CACAAGGACT TCCAAGCAAT TGCCTGCAGT 
GTAAAGCAAC TGGCGGATAC CCTTCTCTCA CAGAGTCCGT CCGAGAATGC CCGGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1A ( NM_001031986.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001027157.2 Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant I, mRNA.

Price & Availability↑

CloneID	OFa07427
Clone ID Related Accession (Same CDS sequence)	NM_001031986.2
Accession Version	NM_001031986.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PFTAIRE-interacting factor 1A, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001031986.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGCAACG AGGAATCCTC CCCGGGAGGC AGCCCCCGCC TCTCCTCGGG CTACACTTCT 
TCTATACACC AGGATCGAGA TTTCAGTGCC AAGATCCAAA CAACGGAGTT TGACCTCGGT 
CAAGCGGGAT TTACCGATTC CGGCGAGGAA CGGGAGATCG TTTACCTAAA GGAAGAGGTC 
AAGAGTCTGC GTAGCCAACT GAAAGAGCTA AATGCCCGGC ACTACGAAGC CATGGAAACA 
GCAGACTCAC ACTGGGTAGA TCTGGAGCAG CAGTACAAGG AGCGCGAGGA GGCCCAACAG 
GCCAAGGAGG CCAGTCTCAA GCAGAAGATA GCCCAACTGC AAGATTGCCT CCGGGAGGAT 
TCCCGAGCGG CGACCGAAAA GATCCAGCAG CTGGAGGAGG GGGAACTGTC ATTGAAGTCC 
TGCCTGGTCC GCATGACCAA GGAGCATCGC GACCTGCTCA CCGAGAATCG CACTCTGCAG 
TGCAGCTTGG AGAGTCTAAT GGCCAAAATG GAAAAGGAGG CGGAACACAA GATGCCCCTA 
ACAGAGGCTC TAGAAAACGA GAGGCGCAAA ACCCAGGCAC TGATGGATGA CCTTATCTTT 
GCCAAGAAGG TGCAGCAGAA CACCGAGGAT CAGTTGAGGC AGGAGACGGA TGCTTTGCGC 
ACCCAGATCT TCAACATCAA AAAGGATTAC CTGCACATCG AGGTCACAAA TGGCGAGCTG 
AAAGAGGAGG TGGGCACGCT GGAGAACAAA ATCCGCCAGA TGGAGAACCA AATGAGGGAT 
TCGGAGGAGC GGGCGCGCTG CCTGGAGGAC GAGCTGCGAA CCAAGGACGA ACAATGCCAG 
CTCTTGGAAC GCAAACTGGG CGTTATGCCC GAGGGCTACA GCCTGGCCGA TGAACTCCAT 
GATAGTCCTG CGAAGCGGGC CAAGAAGGAT GAGGTACCGA GCCTCCAGGC TGCCAGCCAG 
GGACTGGGCG TGGCCCTACT CGATCTCGAG GGTCGTGATT TTGGTCTACC ACTGCACAAG 
GACTTCCAAG CAATTGCCTG CAGTGTAAAG CAACTGGCGG ATACCCTTCT CTCACAGAGT 
CCGTCCGAGG TGAGTCTCCA AAGCAAAGAG CCCGCCAAGC CAGCTGGTTC GGAACCCGAA 
GGCTCCAGTT GGGCTACGGT CCAGGATGCC TCCTCAGAAA AAATACCTGA GACAGGACTA 
TCAACTGTGA CTAGTCTAGA ATGCCAGGGA AATCAAGAAA CTCAAGATAA TCAGGTCATT 
CAAGAAAATC CGGGAAGTCT AGAAAGTCAA GTCGATTCAG AATATCTGGA AAGTCAAGAA 
AGAAATCTCG TTAGCAGCAA GAAATCTAGT TATGTTAGTG TCGTGACATC CTCATGCCGG 
TCAATAGTAC GGGAATTAGC TGGGGGATCT GCCCAAAAAT CTGAACTACA CCTTGGTGAA 
TCAGACAGCA GGGCGAATCC GCAGATAATT CGACCAACAG ATGAGCAAGA GAAGAAGATC 
ACCGCGGTGC CCTATTCCAT GAGACAGATG TTCAACGAGG TTTGTTCTGT GGATCAGCAG 
CACGAACCCG AGCTCAAAAA GAAAAGCAAA AAGCGAGCAA AGAATAAGAA GGAGAAGCAT 
CCAAGACCAA ACAGTCACCA GCGAAAAATA CAGCGTTTCC TCTTCCGTCA CATTTACCGT 
CCACGTACGC TCAATCGAAC TCTGGGTTTC CCTCTTCAAA AGAGCCTCTC CTGCAGTAGT 
TTCCACTCTC TGTTGTCAGT TCACTCGGTT CACTCGGCCA GATCCGGAAT TTCCCTGCAC 
TCCCTTCACG CTGAAGTTGT TAATCTTAAT TGGGAATCTC TGGCCAGCGT GTCATTTGTA 
CAAACGGACG AGGTGAAAAC CCTGGAGGAG GAGGTTCAGG TCACGACACA GTGCAATGAA 
AAGGAGATGC AAGTTCAACC AGAGACGATT GAGTGTGGAA CACAGGTCGA TGTGGGTGTG 
GAGCAACGAC TGCTCCTGAT GCCTCTGAGA ACCAGGTGCT TCGTGGATTT GATTCAGGGC 
GCCGTGGACG GATACCTCGA TGTTGCCGCA TTCCGGACCC GTCTTCTTCA CTTATGGGAG 
GGCGGAGAGG ATTTCCGTGA GCATCTTGAA TTTTTGCAAG ACATGTGGAT TGAAAAGAGT 
ACTCAGAAGA CGCAGGAGAG CTATGAAGGA TTTGTGGCTG CTCTGGACTG TGTCCATAAC 
CTAAAGTTGG CCCTTAAGCC TGCATCTTCG GCGCTTTTGC AACAAATCGA GCAGCGAATC 
CACACAAGAC TTCTGCTCTT CCATAACCAC AAGGCGGAAT CGGAGTTCTG CTGCACTGTC 
TACATTCCCG TGGAGAGGAG CAATTCCACC TTGTTCAGAG CTAAGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001027157.2
CDS	826..3216
Translation	MGNEESSPGGSPRLSSGYTSSIHQDRDFSAKIQTTEFDLGQAGFTDSGEEREIVYLKEEVKSLRSQLKELNARHYEAMETADSHWVDLEQQYKEREEAQQAKEASLKQKIAQLQDCLREDSRAATEKIQQLEEGELSLKSCLVRMTKEHRDLLTENRTLQCSLESLMAKMEKEAEHKMPLTEALENERRKTQALMDDLIFAKKVQQNTEDQLRQETDALRTQIFNIKKDYLHIEVTNGELKEEVGTLENKIRQMENQMRDSEERARCLEDELRTKDEQCQLLERKLGVMPEGYSLADELHDSPAKRAKKDEVPSLQAASQGLGVALLDLEGRDFGLPLHKDFQAIACSVKQLADTLLSQSPSEVSLQSKEPAKPAGSEPEGSSWATVQDASSEKIPETGLSTVTSLECQGNQETQDNQVIQENPGSLESQVDSEYLESQERNLVSSKKSSYVSVVTSSCRSIVRELAGGSAQKSELHLGESDSRANPQIIRPTDEQEKKITAVPYSMRQMFNEVCSVDQQHEPELKKKSKKRAKNKKEKHPRPNSHQRKIQRFLFRHIYRPRTLNRTLGFPLQKSLSCSSFHSLLSVHSVHSARSGISLHSLHAEVVNLNWESLASVSFVQTDEVKTLEEEVQVTTQCNEKEMQVQPETIECGTQVDVGVEQRLLLMPLRTRCFVDLIQGAVDGYLDVAAFRTRLLHLWEGGEDFREHLEFLQDMWIEKSTQKTQESYEGFVAALDCVHNLKLALKPASSALLQQIEQRIHTRLLLFHNHKAESEFCCTVYIPVERSNSTLFRAKK

Target ORF information:

RefSeq Version	NM_001031986.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant I, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031986.2

ORF Insert Sequence:

ATGGGCAACG AGGAATCCTC CCCGGGAGGC AGCCCCCGCC TCTCCTCGGG CTACACTTCT 
TCTATACACC AGGATCGAGA TTTCAGTGCC AAGATCCAAA CAACGGAGTT TGACCTCGGT 
CAAGCGGGAT TTACCGATTC CGGCGAGGAA CGGGAGATCG TTTACCTAAA GGAAGAGGTC 
AAGAGTCTGC GTAGCCAACT GAAAGAGCTA AATGCCCGGC ACTACGAAGC CATGGAAACA 
GCAGACTCAC ACTGGGTAGA TCTGGAGCAG CAGTACAAGG AGCGCGAGGA GGCCCAACAG 
GCCAAGGAGG CCAGTCTCAA GCAGAAGATA GCCCAACTGC AAGATTGCCT CCGGGAGGAT 
TCCCGAGCGG CGACCGAAAA GATCCAGCAG CTGGAGGAGG GGGAACTGTC ATTGAAGTCC 
TGCCTGGTCC GCATGACCAA GGAGCATCGC GACCTGCTCA CCGAGAATCG CACTCTGCAG 
TGCAGCTTGG AGAGTCTAAT GGCCAAAATG GAAAAGGAGG CGGAACACAA GATGCCCCTA 
ACAGAGGCTC TAGAAAACGA GAGGCGCAAA ACCCAGGCAC TGATGGATGA CCTTATCTTT 
GCCAAGAAGG TGCAGCAGAA CACCGAGGAT CAGTTGAGGC AGGAGACGGA TGCTTTGCGC 
ACCCAGATCT TCAACATCAA AAAGGATTAC CTGCACATCG AGGTCACAAA TGGCGAGCTG 
AAAGAGGAGG TGGGCACGCT GGAGAACAAA ATCCGCCAGA TGGAGAACCA AATGAGGGAT 
TCGGAGGAGC GGGCGCGCTG CCTGGAGGAC GAGCTGCGAA CCAAGGACGA ACAATGCCAG 
CTCTTGGAAC GCAAACTGGG CGTTATGCCC GAGGGCTACA GCCTGGCCGA TGAACTCCAT 
GATAGTCCTG CGAAGCGGGC CAAGAAGGAT GAGGTACCGA GCCTCCAGGC TGCCAGCCAG 
GGACTGGGCG TGGCCCTACT CGATCTCGAG GGTCGTGATT TTGGTCTACC ACTGCACAAG 
GACTTCCAAG CAATTGCCTG CAGTGTAAAG CAACTGGCGG ATACCCTTCT CTCACAGAGT 
CCGTCCGAGG TGAGTCTCCA AAGCAAAGAG CCCGCCAAGC CAGCTGGTTC GGAACCCGAA 
GGCTCCAGTT GGGCTACGGT CCAGGATGCC TCCTCAGAAA AAATACCTGA GACAGGACTA 
TCAACTGTGA CTAGTCTAGA ATGCCAGGGA AATCAAGAAA CTCAAGATAA TCAGGTCATT 
CAAGAAAATC CGGGAAGTCT AGAAAGTCAA GTCGATTCAG AATATCTGGA AAGTCAAGAA 
AGAAATCTCG TTAGCAGCAA GAAATCTAGT TATGTTAGTG TCGTGACATC CTCATGCCGG 
TCAATAGTAC GGGAATTAGC TGGGGGATCT GCCCAAAAAT CTGAACTACA CCTTGGTGAA 
TCAGACAGCA GGGCGAATCC GCAGATAATT CGACCAACAG ATGAGCAAGA GAAGAAGATC 
ACCGCGGTGC CCTATTCCAT GAGACAGATG TTCAACGAGG TTTGTTCTGT GGATCAGCAG 
CACGAACCCG AGCTCAAAAA GAAAAGCAAA AAGCGAGCAA AGAATAAGAA GGAGAAGCAT 
CCAAGACCAA ACAGTCACCA GCGAAAAATA CAGCGTTTCC TCTTCCGTCA CATTTACCGT 
CCACGTACGC TCAATCGAAC TCTGGGTTTC CCTCTTCAAA AGAGCCTCTC CTGCAGTAGT 
TTCCACTCTC TGTTGTCAGT TCACTCGGTT CACTCGGCCA GATCCGGAAT TTCCCTGCAC 
TCCCTTCACG CTGAAGTTGT TAATCTTAAT TGGGAATCTC TGGCCAGCGT GTCATTTGTA 
CAAACGGACG AGGTGAAAAC CCTGGAGGAG GAGGTTCAGG TCACGACACA GTGCAATGAA 
AAGGAGATGC AAGTTCAACC AGAGACGATT GAGTGTGGAA CACAGGTCGA TGTGGGTGTG 
GAGCAACGAC TGCTCCTGAT GCCTCTGAGA ACCAGGTGCT TCGTGGATTT GATTCAGGGC 
GCCGTGGACG GATACCTCGA TGTTGCCGCA TTCCGGACCC GTCTTCTTCA CTTATGGGAG 
GGCGGAGAGG ATTTCCGTGA GCATCTTGAA TTTTTGCAAG ACATGTGGAT TGAAAAGAGT 
ACTCAGAAGA CGCAGGAGAG CTATGAAGGA TTTGTGGCTG CTCTGGACTG TGTCCATAAC 
CTAAAGTTGG CCCTTAAGCC TGCATCTTCG GCGCTTTTGC AACAAATCGA GCAGCGAATC 
CACACAAGAC TTCTGCTCTT CCATAACCAC AAGGCGGAAT CGGAGTTCTG CTGCACTGTC 
TACATTCCCG TGGAGAGGAG CAATTCCACC TTGTTCAGAG CTAAGAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1A ( NM_001202266.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001189195.2 Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant H, mRNA.

Price & Availability↑

CloneID	OFa07701
Clone ID Related Accession (Same CDS sequence)	NM_001202266.2 , NM_001316487.1
Accession Version	NM_001202266.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PFTAIRE-interacting factor 1A, isoform H
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001202266.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG ATTATGGGGC AGGCTGATAG ACTCAAGGCT 
GCCGATATGC GGCAATTGAA GAACGCGTAC GAAAACCGAT TGACTCACAT ACAGAAATCA 
GCCAAAATTG AAATAACACG TTTGTTAGAG GAAATAAAGA CCAAAGAACG TAATATAGGA 
CAACTGAGGA AGGACTTGCT GGCACTGCAG AGTCCCAAGA AGCTGAAGCA GAATGAAGCC 
AAAGTGCCAG CGACGACGAA ACCCAAAACG AACCCCAACG AAACGAGCCA AAAGCCAAAG 
AAACCCACTA CGAAGGCGGC GGGAGAAACA AAAGCTAAAA CCGAACCAGA TACAGATACA 
GATATATCCG AGCAAAATCC CATACGCCAG GCTCAAGTTA CACCTGAGAT AACCCAACTC 
AAGAACATTG AGCTAATCAA CAATACGAAA AATGCACATA AATCAGCGCT CGAGATGCGC 
ACAACAATGA AGAGCAGTCA GCCGAATGGA TTGGATGATG CTCCTACACG GATGAGGGCC 
CCCGACGAGA TGAGGGCCAC CACTTGGATG ACGACGCTGG TAGGCAGTAA CTGCCATAGC 
CACAGCCACA GCCATCCGGC AGTAGCCAAG TCGAAGGATC CCAATTCGGA CACGGACTCG 
GCCTTGTCCT CGGCCCCGCC CTCCATCAGC CCGCAGCCGC CATCCAGCGG ATCCGACAGC 
GGCGTGATTT GGCAAACACT GCAGGACCTG GACAAGTTGC AAAAGGAAGC GGAAATGTAC 
CAAAAGGAGA ACGAACGCCT GCAGACGGAG GTGCAGCTGA TGAAACAGGA GCTGGATGCC 
GCGGAAAAGG CGGCCATAAG CAGGGCCAAG AAGCAGGCCC AGATCGGGGA GCTGATGCAG 
CGCATCAAGG AGCTGGAAGA GATGCAGTCG AGCTTGGAGG ACGAGGCCAG CGAACTGAGG 
GAGCAGAATG AACTCCTTGA GTTTCGCATA CTCGAGCTGG AAGATGATAG CGATAAGATG 
GAGAGCACCT GCGAGGGCCA CTGCCAGAGT CTGCAGGATC TGCTGGAGCA GTCTGCCCAG 
CGGCTGGAGG ATCGGGACAA GCGCTGCTTG CAACAATTGC TGCAGTGCGT CCAGCAACTG 
GATCTGGATA CCATGATGCC AGGCGATCAG AACCGGACGG AAACTCCCAA AAGGCACAAT 
TCAACCCACA GTCACAGTCA CAGTCACAGC CACAGTCAAA GTCACAACCA CAGGATCGTA 
GCCACAGTTC AGCCGTATAG CAACTGTGCC ACGCCCACAG CCCCGCCCAC GCCAAGCAAG 
AGGCACTGCA ACCAGGTGAG CTCGTGGCAG AGCAGCAGCC TCAGCGAGAG CGGAGTGTTC 
GTAGAGTGCG ATCTGCTTTC GAATCCCAAT CCCATTGGCC ACCCGCATCT GCAGTTCTAT 
CAGGAGAGAT TGGAGCAGTT GGAGGGCAAG CTGCTGATCT ACGAGAGCAG CGGCGAGACC 
CAGGCACGGC ACTTGGCCCA GAGACTGCAG CGGGAACTGC AGCTGGAGAA GGATCTCAAA 
GAGCTTGCGG ACCGAGTGGA ATTGCTAATC GAGCAAAACG CCCAGTTGGA GGAGGCCAAG 
TGCGAGTTTG AGGAGGCCGA GAACGATACA CGACTCCATT TGCAGCGTAA CGAGGTGGAA 
CTTGAAATTC TTCGCCAGCG CAACGTGGAG TTGGAATTCG GCAAGGAAGC ACTAGGCGCC 
AAGTATCAGG ATTGCAGGGC GGAGGTACTA ATCCTTCGAG AGGATCTAGC GGCAGCTGAA 
ACCCAACTGG AGCATCTGCA GGCCGAAAGG AAGCAAGCCA GAAAGGAGCT TCAGGATCTG 
AGAAGATCGC TTCCATTACT CCTAATCTTC CGCTTGTTAT CACTAGCCAA AATGGGCAAC 
GAGGAATCCT CCCCGGGAGG CAGCCCCCGC CTCTCCTCGG GCTACACTTC TTCTATACAC 
CAGGATCGAG ATTTCAGTGC CAAGATCCAA ACAACGGAGT TTGACCTCGG TCAAGCGGGA 
TTTACCGATT CCGGCGAGGA ACGGGAGATC GTTTACCTAA AGGAAGAGGT CAAGAGTCTG 
CGTAGCCAAC TGAAAGAGCT AAATGCCCGG CACTACGAAG CCATGGAAAC AGCAGACTCA 
CACTGGGTAG ATCTGGAGCA GCAGTACAAG GAGCGCGAGG AGGCCCAACA GGCCAAGGAG 
GCCAGTCTCA AGCAGAAGAT AGCCCAACTG CAAGATTGCC TCCGGGAGGA TTCCCGAGCG 
GCGACCGAAA AGATCCAGCA GCTGGAGGAG GGGGAACTGT CATTGAAGTC CTGCCTGGTC 
CGCATGACCA AGGAGCATCG CGACCTGCTC ACCGAGAATC GCACTCTGCA GTGCAGCTTG 
GAGAGTCTAA TGGCCAAAAT GGAAAAGGAG GCGGAACACA AGATGCCCCT AACAGAGGCT 
CTAGAAAACG AGAGGCGCAA AACCCAGGCA CTGATGGATG ACCTTATCTT TGCCAAGAAG 
GTGCAGCAGA ACACCGAGGA TCAGTTGAGG CAGGAGACGG ATGCTTTGCG CACCCAGATC 
TTCAACATCA AAAAGGATTA CCTGCACATC GAGGTCACAA ATGGCGAGCT GAAAGAGGAG 
GTGGGCACGC TGGAGAACAA AATCCGCCAG ATGGAGAACC AAATGAGGGA TTCGGAGGAG 
CGGGCGCGCT GCCTGGAGGA CGAGCTGCGA ACCAAGGACG AACAATGCCA GCTCTTGGAA 
CGCAAACTGG GCGTTATGCC CGAGGGCTAC AGCCTGGCCG ATGAACTCCA TGATAGTCCT 
GCGAAGCGGG CCAAGAAGGA TGAGGTACCG AGCCTCCAGG CTGCCAGCCA GGGACTGGGC 
GTGGCCCTAC TCGATCTCGA GGGTCGTGAT TTTGGTCTAC CACTGCACAA GGACTTCCAA 
GCAATTGCCT GCAGTGTAAA GCAACTGGCG GATACCCTTC TCTCACAGAG TCCGTCCGAG 
AATGCCCGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189195.2
CDS	448..4242
Translation	MAENQTKTTSSKGCNTMSRTAHILAPHNGTVVRRTDPGSKLKESFHTEKHDIEDWQTMAKNKLKRKKKLSSSLTSVGDTVNQEKRTRIEGIQLHCRGKLRPKETQSFEAEKGPSAGSQKQLGKAEKDRERPVIIGGVTTTNVIAAPPQRIKRKLQENMDTIQKLNALTEQLRLEVNELKSSLITERGAVRALRAQNDADSRRWKSEVKKLQHTLEMAKKSNGIKKAIESGPESSGAHVTADGLINYEIQRLTNEIAVLKEANRNKEEKTQIMGQADRLKAADMRQLKNAYENRLTHIQKSAKIEITRLLEEIKTKERNIGQLRKDLLALQSPKKLKQNEAKVPATTKPKTNPNETSQKPKKPTTKAAGETKAKTEPDTDTDISEQNPIRQAQVTPEITQLKNIELINNTKNAHKSALEMRTTMKSSQPNGLDDAPTRMRAPDEMRATTWMTTLVGSNCHSHSHSHPAVAKSKDPNSDTDSALSSAPPSISPQPPSSGSDSGVIWQTLQDLDKLQKEAEMYQKENERLQTEVQLMKQELDAAEKAAISRAKKQAQIGELMQRIKELEEMQSSLEDEASELREQNELLEFRILELEDDSDKMESTCEGHCQSLQDLLEQSAQRLEDRDKRCLQQLLQCVQQLDLDTMMPGDQNRTETPKRHNSTHSHSHSHSHSQSHNHRIVATVQPYSNCATPTAPPTPSKRHCNQVSSWQSSSLSESGVFVECDLLSNPNPIGHPHLQFYQERLEQLEGKLLIYESSGETQARHLAQRLQRELQLEKDLKELADRVELLIEQNAQLEEAKCEFEEAENDTRLHLQRNEVELEILRQRNVELEFGKEALGAKYQDCRAEVLILREDLAAAETQLEHLQAERKQARKELQDLRRSLPLLLIFRLLSLAKMGNEESSPGGSPRLSSGYTSSIHQDRDFSAKIQTTEFDLGQAGFTDSGEEREIVYLKEEVKSLRSQLKELNARHYEAMETADSHWVDLEQQYKEREEAQQAKEASLKQKIAQLQDCLREDSRAATEKIQQLEEGELSLKSCLVRMTKEHRDLLTENRTLQCSLESLMAKMEKEAEHKMPLTEALENERRKTQALMDDLIFAKKVQQNTEDQLRQETDALRTQIFNIKKDYLHIEVTNGELKEEVGTLENKIRQMENQMRDSEERARCLEDELRTKDEQCQLLERKLGVMPEGYSLADELHDSPAKRAKKDEVPSLQAASQGLGVALLDLEGRDFGLPLHKDFQAIACSVKQLADTLLSQSPSENARL

Target ORF information:

RefSeq Version	NM_001202266.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant H, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202266.2

ORF Insert Sequence:

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG ATTATGGGGC AGGCTGATAG ACTCAAGGCT 
GCCGATATGC GGCAATTGAA GAACGCGTAC GAAAACCGAT TGACTCACAT ACAGAAATCA 
GCCAAAATTG AAATAACACG TTTGTTAGAG GAAATAAAGA CCAAAGAACG TAATATAGGA 
CAACTGAGGA AGGACTTGCT GGCACTGCAG AGTCCCAAGA AGCTGAAGCA GAATGAAGCC 
AAAGTGCCAG CGACGACGAA ACCCAAAACG AACCCCAACG AAACGAGCCA AAAGCCAAAG 
AAACCCACTA CGAAGGCGGC GGGAGAAACA AAAGCTAAAA CCGAACCAGA TACAGATACA 
GATATATCCG AGCAAAATCC CATACGCCAG GCTCAAGTTA CACCTGAGAT AACCCAACTC 
AAGAACATTG AGCTAATCAA CAATACGAAA AATGCACATA AATCAGCGCT CGAGATGCGC 
ACAACAATGA AGAGCAGTCA GCCGAATGGA TTGGATGATG CTCCTACACG GATGAGGGCC 
CCCGACGAGA TGAGGGCCAC CACTTGGATG ACGACGCTGG TAGGCAGTAA CTGCCATAGC 
CACAGCCACA GCCATCCGGC AGTAGCCAAG TCGAAGGATC CCAATTCGGA CACGGACTCG 
GCCTTGTCCT CGGCCCCGCC CTCCATCAGC CCGCAGCCGC CATCCAGCGG ATCCGACAGC 
GGCGTGATTT GGCAAACACT GCAGGACCTG GACAAGTTGC AAAAGGAAGC GGAAATGTAC 
CAAAAGGAGA ACGAACGCCT GCAGACGGAG GTGCAGCTGA TGAAACAGGA GCTGGATGCC 
GCGGAAAAGG CGGCCATAAG CAGGGCCAAG AAGCAGGCCC AGATCGGGGA GCTGATGCAG 
CGCATCAAGG AGCTGGAAGA GATGCAGTCG AGCTTGGAGG ACGAGGCCAG CGAACTGAGG 
GAGCAGAATG AACTCCTTGA GTTTCGCATA CTCGAGCTGG AAGATGATAG CGATAAGATG 
GAGAGCACCT GCGAGGGCCA CTGCCAGAGT CTGCAGGATC TGCTGGAGCA GTCTGCCCAG 
CGGCTGGAGG ATCGGGACAA GCGCTGCTTG CAACAATTGC TGCAGTGCGT CCAGCAACTG 
GATCTGGATA CCATGATGCC AGGCGATCAG AACCGGACGG AAACTCCCAA AAGGCACAAT 
TCAACCCACA GTCACAGTCA CAGTCACAGC CACAGTCAAA GTCACAACCA CAGGATCGTA 
GCCACAGTTC AGCCGTATAG CAACTGTGCC ACGCCCACAG CCCCGCCCAC GCCAAGCAAG 
AGGCACTGCA ACCAGGTGAG CTCGTGGCAG AGCAGCAGCC TCAGCGAGAG CGGAGTGTTC 
GTAGAGTGCG ATCTGCTTTC GAATCCCAAT CCCATTGGCC ACCCGCATCT GCAGTTCTAT 
CAGGAGAGAT TGGAGCAGTT GGAGGGCAAG CTGCTGATCT ACGAGAGCAG CGGCGAGACC 
CAGGCACGGC ACTTGGCCCA GAGACTGCAG CGGGAACTGC AGCTGGAGAA GGATCTCAAA 
GAGCTTGCGG ACCGAGTGGA ATTGCTAATC GAGCAAAACG CCCAGTTGGA GGAGGCCAAG 
TGCGAGTTTG AGGAGGCCGA GAACGATACA CGACTCCATT TGCAGCGTAA CGAGGTGGAA 
CTTGAAATTC TTCGCCAGCG CAACGTGGAG TTGGAATTCG GCAAGGAAGC ACTAGGCGCC 
AAGTATCAGG ATTGCAGGGC GGAGGTACTA ATCCTTCGAG AGGATCTAGC GGCAGCTGAA 
ACCCAACTGG AGCATCTGCA GGCCGAAAGG AAGCAAGCCA GAAAGGAGCT TCAGGATCTG 
AGAAGATCGC TTCCATTACT CCTAATCTTC CGCTTGTTAT CACTAGCCAA AATGGGCAAC 
GAGGAATCCT CCCCGGGAGG CAGCCCCCGC CTCTCCTCGG GCTACACTTC TTCTATACAC 
CAGGATCGAG ATTTCAGTGC CAAGATCCAA ACAACGGAGT TTGACCTCGG TCAAGCGGGA 
TTTACCGATT CCGGCGAGGA ACGGGAGATC GTTTACCTAA AGGAAGAGGT CAAGAGTCTG 
CGTAGCCAAC TGAAAGAGCT AAATGCCCGG CACTACGAAG CCATGGAAAC AGCAGACTCA 
CACTGGGTAG ATCTGGAGCA GCAGTACAAG GAGCGCGAGG AGGCCCAACA GGCCAAGGAG 
GCCAGTCTCA AGCAGAAGAT AGCCCAACTG CAAGATTGCC TCCGGGAGGA TTCCCGAGCG 
GCGACCGAAA AGATCCAGCA GCTGGAGGAG GGGGAACTGT CATTGAAGTC CTGCCTGGTC 
CGCATGACCA AGGAGCATCG CGACCTGCTC ACCGAGAATC GCACTCTGCA GTGCAGCTTG 
GAGAGTCTAA TGGCCAAAAT GGAAAAGGAG GCGGAACACA AGATGCCCCT AACAGAGGCT 
CTAGAAAACG AGAGGCGCAA AACCCAGGCA CTGATGGATG ACCTTATCTT TGCCAAGAAG 
GTGCAGCAGA ACACCGAGGA TCAGTTGAGG CAGGAGACGG ATGCTTTGCG CACCCAGATC 
TTCAACATCA AAAAGGATTA CCTGCACATC GAGGTCACAA ATGGCGAGCT GAAAGAGGAG 
GTGGGCACGC TGGAGAACAA AATCCGCCAG ATGGAGAACC AAATGAGGGA TTCGGAGGAG 
CGGGCGCGCT GCCTGGAGGA CGAGCTGCGA ACCAAGGACG AACAATGCCA GCTCTTGGAA 
CGCAAACTGG GCGTTATGCC CGAGGGCTAC AGCCTGGCCG ATGAACTCCA TGATAGTCCT 
GCGAAGCGGG CCAAGAAGGA TGAGGTACCG AGCCTCCAGG CTGCCAGCCA GGGACTGGGC 
GTGGCCCTAC TCGATCTCGA GGGTCGTGAT TTTGGTCTAC CACTGCACAA GGACTTCCAA 
GCAATTGCCT GCAGTGTAAA GCAACTGGCG GATACCCTTC TCTCACAGAG TCCGTCCGAG 
AATGCCCGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1A ( NM_001316487.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001303416.1 Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant J, mRNA.

Price & Availability↑

CloneID	OFa07701
Clone ID Related Accession (Same CDS sequence)	NM_001202266.2 , NM_001316487.1
Accession Version	NM_001316487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PFTAIRE-interacting factor 1A, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG ATTATGGGGC AGGCTGATAG ACTCAAGGCT 
GCCGATATGC GGCAATTGAA GAACGCGTAC GAAAACCGAT TGACTCACAT ACAGAAATCA 
GCCAAAATTG AAATAACACG TTTGTTAGAG GAAATAAAGA CCAAAGAACG TAATATAGGA 
CAACTGAGGA AGGACTTGCT GGCACTGCAG AGTCCCAAGA AGCTGAAGCA GAATGAAGCC 
AAAGTGCCAG CGACGACGAA ACCCAAAACG AACCCCAACG AAACGAGCCA AAAGCCAAAG 
AAACCCACTA CGAAGGCGGC GGGAGAAACA AAAGCTAAAA CCGAACCAGA TACAGATACA 
GATATATCCG AGCAAAATCC CATACGCCAG GCTCAAGTTA CACCTGAGAT AACCCAACTC 
AAGAACATTG AGCTAATCAA CAATACGAAA AATGCACATA AATCAGCGCT CGAGATGCGC 
ACAACAATGA AGAGCAGTCA GCCGAATGGA TTGGATGATG CTCCTACACG GATGAGGGCC 
CCCGACGAGA TGAGGGCCAC CACTTGGATG ACGACGCTGG TAGGCAGTAA CTGCCATAGC 
CACAGCCACA GCCATCCGGC AGTAGCCAAG TCGAAGGATC CCAATTCGGA CACGGACTCG 
GCCTTGTCCT CGGCCCCGCC CTCCATCAGC CCGCAGCCGC CATCCAGCGG ATCCGACAGC 
GGCGTGATTT GGCAAACACT GCAGGACCTG GACAAGTTGC AAAAGGAAGC GGAAATGTAC 
CAAAAGGAGA ACGAACGCCT GCAGACGGAG GTGCAGCTGA TGAAACAGGA GCTGGATGCC 
GCGGAAAAGG CGGCCATAAG CAGGGCCAAG AAGCAGGCCC AGATCGGGGA GCTGATGCAG 
CGCATCAAGG AGCTGGAAGA GATGCAGTCG AGCTTGGAGG ACGAGGCCAG CGAACTGAGG 
GAGCAGAATG AACTCCTTGA GTTTCGCATA CTCGAGCTGG AAGATGATAG CGATAAGATG 
GAGAGCACCT GCGAGGGCCA CTGCCAGAGT CTGCAGGATC TGCTGGAGCA GTCTGCCCAG 
CGGCTGGAGG ATCGGGACAA GCGCTGCTTG CAACAATTGC TGCAGTGCGT CCAGCAACTG 
GATCTGGATA CCATGATGCC AGGCGATCAG AACCGGACGG AAACTCCCAA AAGGCACAAT 
TCAACCCACA GTCACAGTCA CAGTCACAGC CACAGTCAAA GTCACAACCA CAGGATCGTA 
GCCACAGTTC AGCCGTATAG CAACTGTGCC ACGCCCACAG CCCCGCCCAC GCCAAGCAAG 
AGGCACTGCA ACCAGGTGAG CTCGTGGCAG AGCAGCAGCC TCAGCGAGAG CGGAGTGTTC 
GTAGAGTGCG ATCTGCTTTC GAATCCCAAT CCCATTGGCC ACCCGCATCT GCAGTTCTAT 
CAGGAGAGAT TGGAGCAGTT GGAGGGCAAG CTGCTGATCT ACGAGAGCAG CGGCGAGACC 
CAGGCACGGC ACTTGGCCCA GAGACTGCAG CGGGAACTGC AGCTGGAGAA GGATCTCAAA 
GAGCTTGCGG ACCGAGTGGA ATTGCTAATC GAGCAAAACG CCCAGTTGGA GGAGGCCAAG 
TGCGAGTTTG AGGAGGCCGA GAACGATACA CGACTCCATT TGCAGCGTAA CGAGGTGGAA 
CTTGAAATTC TTCGCCAGCG CAACGTGGAG TTGGAATTCG GCAAGGAAGC ACTAGGCGCC 
AAGTATCAGG ATTGCAGGGC GGAGGTACTA ATCCTTCGAG AGGATCTAGC GGCAGCTGAA 
ACCCAACTGG AGCATCTGCA GGCCGAAAGG AAGCAAGCCA GAAAGGAGCT TCAGGATCTG 
AGAAGATCGC TTCCATTACT CCTAATCTTC CGCTTGTTAT CACTAGCCAA AATGGGCAAC 
GAGGAATCCT CCCCGGGAGG CAGCCCCCGC CTCTCCTCGG GCTACACTTC TTCTATACAC 
CAGGATCGAG ATTTCAGTGC CAAGATCCAA ACAACGGAGT TTGACCTCGG TCAAGCGGGA 
TTTACCGATT CCGGCGAGGA ACGGGAGATC GTTTACCTAA AGGAAGAGGT CAAGAGTCTG 
CGTAGCCAAC TGAAAGAGCT AAATGCCCGG CACTACGAAG CCATGGAAAC AGCAGACTCA 
CACTGGGTAG ATCTGGAGCA GCAGTACAAG GAGCGCGAGG AGGCCCAACA GGCCAAGGAG 
GCCAGTCTCA AGCAGAAGAT AGCCCAACTG CAAGATTGCC TCCGGGAGGA TTCCCGAGCG 
GCGACCGAAA AGATCCAGCA GCTGGAGGAG GGGGAACTGT CATTGAAGTC CTGCCTGGTC 
CGCATGACCA AGGAGCATCG CGACCTGCTC ACCGAGAATC GCACTCTGCA GTGCAGCTTG 
GAGAGTCTAA TGGCCAAAAT GGAAAAGGAG GCGGAACACA AGATGCCCCT AACAGAGGCT 
CTAGAAAACG AGAGGCGCAA AACCCAGGCA CTGATGGATG ACCTTATCTT TGCCAAGAAG 
GTGCAGCAGA ACACCGAGGA TCAGTTGAGG CAGGAGACGG ATGCTTTGCG CACCCAGATC 
TTCAACATCA AAAAGGATTA CCTGCACATC GAGGTCACAA ATGGCGAGCT GAAAGAGGAG 
GTGGGCACGC TGGAGAACAA AATCCGCCAG ATGGAGAACC AAATGAGGGA TTCGGAGGAG 
CGGGCGCGCT GCCTGGAGGA CGAGCTGCGA ACCAAGGACG AACAATGCCA GCTCTTGGAA 
CGCAAACTGG GCGTTATGCC CGAGGGCTAC AGCCTGGCCG ATGAACTCCA TGATAGTCCT 
GCGAAGCGGG CCAAGAAGGA TGAGGTACCG AGCCTCCAGG CTGCCAGCCA GGGACTGGGC 
GTGGCCCTAC TCGATCTCGA GGGTCGTGAT TTTGGTCTAC CACTGCACAA GGACTTCCAA 
GCAATTGCCT GCAGTGTAAA GCAACTGGCG GATACCCTTC TCTCACAGAG TCCGTCCGAG 
AATGCCCGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303416.1
CDS	534..4328
Translation	MAENQTKTTSSKGCNTMSRTAHILAPHNGTVVRRTDPGSKLKESFHTEKHDIEDWQTMAKNKLKRKKKLSSSLTSVGDTVNQEKRTRIEGIQLHCRGKLRPKETQSFEAEKGPSAGSQKQLGKAEKDRERPVIIGGVTTTNVIAAPPQRIKRKLQENMDTIQKLNALTEQLRLEVNELKSSLITERGAVRALRAQNDADSRRWKSEVKKLQHTLEMAKKSNGIKKAIESGPESSGAHVTADGLINYEIQRLTNEIAVLKEANRNKEEKTQIMGQADRLKAADMRQLKNAYENRLTHIQKSAKIEITRLLEEIKTKERNIGQLRKDLLALQSPKKLKQNEAKVPATTKPKTNPNETSQKPKKPTTKAAGETKAKTEPDTDTDISEQNPIRQAQVTPEITQLKNIELINNTKNAHKSALEMRTTMKSSQPNGLDDAPTRMRAPDEMRATTWMTTLVGSNCHSHSHSHPAVAKSKDPNSDTDSALSSAPPSISPQPPSSGSDSGVIWQTLQDLDKLQKEAEMYQKENERLQTEVQLMKQELDAAEKAAISRAKKQAQIGELMQRIKELEEMQSSLEDEASELREQNELLEFRILELEDDSDKMESTCEGHCQSLQDLLEQSAQRLEDRDKRCLQQLLQCVQQLDLDTMMPGDQNRTETPKRHNSTHSHSHSHSHSQSHNHRIVATVQPYSNCATPTAPPTPSKRHCNQVSSWQSSSLSESGVFVECDLLSNPNPIGHPHLQFYQERLEQLEGKLLIYESSGETQARHLAQRLQRELQLEKDLKELADRVELLIEQNAQLEEAKCEFEEAENDTRLHLQRNEVELEILRQRNVELEFGKEALGAKYQDCRAEVLILREDLAAAETQLEHLQAERKQARKELQDLRRSLPLLLIFRLLSLAKMGNEESSPGGSPRLSSGYTSSIHQDRDFSAKIQTTEFDLGQAGFTDSGEEREIVYLKEEVKSLRSQLKELNARHYEAMETADSHWVDLEQQYKEREEAQQAKEASLKQKIAQLQDCLREDSRAATEKIQQLEEGELSLKSCLVRMTKEHRDLLTENRTLQCSLESLMAKMEKEAEHKMPLTEALENERRKTQALMDDLIFAKKVQQNTEDQLRQETDALRTQIFNIKKDYLHIEVTNGELKEEVGTLENKIRQMENQMRDSEERARCLEDELRTKDEQCQLLERKLGVMPEGYSLADELHDSPAKRAKKDEVPSLQAASQGLGVALLDLEGRDFGLPLHKDFQAIACSVKQLADTLLSQSPSENARL

Target ORF information:

RefSeq Version	NM_001316487.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant J, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316487.1

ORF Insert Sequence:

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG ATTATGGGGC AGGCTGATAG ACTCAAGGCT 
GCCGATATGC GGCAATTGAA GAACGCGTAC GAAAACCGAT TGACTCACAT ACAGAAATCA 
GCCAAAATTG AAATAACACG TTTGTTAGAG GAAATAAAGA CCAAAGAACG TAATATAGGA 
CAACTGAGGA AGGACTTGCT GGCACTGCAG AGTCCCAAGA AGCTGAAGCA GAATGAAGCC 
AAAGTGCCAG CGACGACGAA ACCCAAAACG AACCCCAACG AAACGAGCCA AAAGCCAAAG 
AAACCCACTA CGAAGGCGGC GGGAGAAACA AAAGCTAAAA CCGAACCAGA TACAGATACA 
GATATATCCG AGCAAAATCC CATACGCCAG GCTCAAGTTA CACCTGAGAT AACCCAACTC 
AAGAACATTG AGCTAATCAA CAATACGAAA AATGCACATA AATCAGCGCT CGAGATGCGC 
ACAACAATGA AGAGCAGTCA GCCGAATGGA TTGGATGATG CTCCTACACG GATGAGGGCC 
CCCGACGAGA TGAGGGCCAC CACTTGGATG ACGACGCTGG TAGGCAGTAA CTGCCATAGC 
CACAGCCACA GCCATCCGGC AGTAGCCAAG TCGAAGGATC CCAATTCGGA CACGGACTCG 
GCCTTGTCCT CGGCCCCGCC CTCCATCAGC CCGCAGCCGC CATCCAGCGG ATCCGACAGC 
GGCGTGATTT GGCAAACACT GCAGGACCTG GACAAGTTGC AAAAGGAAGC GGAAATGTAC 
CAAAAGGAGA ACGAACGCCT GCAGACGGAG GTGCAGCTGA TGAAACAGGA GCTGGATGCC 
GCGGAAAAGG CGGCCATAAG CAGGGCCAAG AAGCAGGCCC AGATCGGGGA GCTGATGCAG 
CGCATCAAGG AGCTGGAAGA GATGCAGTCG AGCTTGGAGG ACGAGGCCAG CGAACTGAGG 
GAGCAGAATG AACTCCTTGA GTTTCGCATA CTCGAGCTGG AAGATGATAG CGATAAGATG 
GAGAGCACCT GCGAGGGCCA CTGCCAGAGT CTGCAGGATC TGCTGGAGCA GTCTGCCCAG 
CGGCTGGAGG ATCGGGACAA GCGCTGCTTG CAACAATTGC TGCAGTGCGT CCAGCAACTG 
GATCTGGATA CCATGATGCC AGGCGATCAG AACCGGACGG AAACTCCCAA AAGGCACAAT 
TCAACCCACA GTCACAGTCA CAGTCACAGC CACAGTCAAA GTCACAACCA CAGGATCGTA 
GCCACAGTTC AGCCGTATAG CAACTGTGCC ACGCCCACAG CCCCGCCCAC GCCAAGCAAG 
AGGCACTGCA ACCAGGTGAG CTCGTGGCAG AGCAGCAGCC TCAGCGAGAG CGGAGTGTTC 
GTAGAGTGCG ATCTGCTTTC GAATCCCAAT CCCATTGGCC ACCCGCATCT GCAGTTCTAT 
CAGGAGAGAT TGGAGCAGTT GGAGGGCAAG CTGCTGATCT ACGAGAGCAG CGGCGAGACC 
CAGGCACGGC ACTTGGCCCA GAGACTGCAG CGGGAACTGC AGCTGGAGAA GGATCTCAAA 
GAGCTTGCGG ACCGAGTGGA ATTGCTAATC GAGCAAAACG CCCAGTTGGA GGAGGCCAAG 
TGCGAGTTTG AGGAGGCCGA GAACGATACA CGACTCCATT TGCAGCGTAA CGAGGTGGAA 
CTTGAAATTC TTCGCCAGCG CAACGTGGAG TTGGAATTCG GCAAGGAAGC ACTAGGCGCC 
AAGTATCAGG ATTGCAGGGC GGAGGTACTA ATCCTTCGAG AGGATCTAGC GGCAGCTGAA 
ACCCAACTGG AGCATCTGCA GGCCGAAAGG AAGCAAGCCA GAAAGGAGCT TCAGGATCTG 
AGAAGATCGC TTCCATTACT CCTAATCTTC CGCTTGTTAT CACTAGCCAA AATGGGCAAC 
GAGGAATCCT CCCCGGGAGG CAGCCCCCGC CTCTCCTCGG GCTACACTTC TTCTATACAC 
CAGGATCGAG ATTTCAGTGC CAAGATCCAA ACAACGGAGT TTGACCTCGG TCAAGCGGGA 
TTTACCGATT CCGGCGAGGA ACGGGAGATC GTTTACCTAA AGGAAGAGGT CAAGAGTCTG 
CGTAGCCAAC TGAAAGAGCT AAATGCCCGG CACTACGAAG CCATGGAAAC AGCAGACTCA 
CACTGGGTAG ATCTGGAGCA GCAGTACAAG GAGCGCGAGG AGGCCCAACA GGCCAAGGAG 
GCCAGTCTCA AGCAGAAGAT AGCCCAACTG CAAGATTGCC TCCGGGAGGA TTCCCGAGCG 
GCGACCGAAA AGATCCAGCA GCTGGAGGAG GGGGAACTGT CATTGAAGTC CTGCCTGGTC 
CGCATGACCA AGGAGCATCG CGACCTGCTC ACCGAGAATC GCACTCTGCA GTGCAGCTTG 
GAGAGTCTAA TGGCCAAAAT GGAAAAGGAG GCGGAACACA AGATGCCCCT AACAGAGGCT 
CTAGAAAACG AGAGGCGCAA AACCCAGGCA CTGATGGATG ACCTTATCTT TGCCAAGAAG 
GTGCAGCAGA ACACCGAGGA TCAGTTGAGG CAGGAGACGG ATGCTTTGCG CACCCAGATC 
TTCAACATCA AAAAGGATTA CCTGCACATC GAGGTCACAA ATGGCGAGCT GAAAGAGGAG 
GTGGGCACGC TGGAGAACAA AATCCGCCAG ATGGAGAACC AAATGAGGGA TTCGGAGGAG 
CGGGCGCGCT GCCTGGAGGA CGAGCTGCGA ACCAAGGACG AACAATGCCA GCTCTTGGAA 
CGCAAACTGG GCGTTATGCC CGAGGGCTAC AGCCTGGCCG ATGAACTCCA TGATAGTCCT 
GCGAAGCGGG CCAAGAAGGA TGAGGTACCG AGCCTCCAGG CTGCCAGCCA GGGACTGGGC 
GTGGCCCTAC TCGATCTCGA GGGTCGTGAT TTTGGTCTAC CACTGCACAA GGACTTCCAA 
GCAATTGCCT GCAGTGTAAA GCAACTGGCG GATACCCTTC TCTCACAGAG TCCGTCCGAG 
AATGCCCGGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pif1A ( NM_001170085.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163556.2 Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant G, mRNA.

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CloneID	OFa07702
Clone ID Related Accession (Same CDS sequence)	NM_001170085.2
Accession Version	NM_001170085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	PFTAIRE-interacting factor 1A, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170085.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG TTAGAGGAAA TAAAGACCAA AGAACGTAAT 
ATAGGACAAC TGAGGAAGGA CTTGCTGGCA CTGCAGAGTC CCAAGAAGCT GAAGCAGAAT 
GAAGCCAAAG TGCCAGCGAC GACGAAACCC AAAACGAACC CCAACGAAAC GAGCCAAAAG 
CCAAAGAAAC CCACTACGAA GGCGGCGGGA GAAACAAAAG CTAAAACCGA ACCAGATACA 
GATACAGATA TATCCGAGCA AAATCCCATA CGCCAGGCTC AAGTTACACC TGAGATAACC 
CAACTCAAGA ACATTGAGCT AATCAACAAT ACGAAAAATG CACATAAATC AGCGCTCGAG 
ATGCGCACAA CAATGAAGAG CAGTCAGCCG AATGGATTGG ATGATGCTCC TACACGGATG 
AGGGCCCCCG ACGAGATGAG GGCCACCACT TGGATGACGA CGCTGGTAGG CAGTAACTGC 
CATAGCCACA GCCACAGCCA TCCGGCAGTA GCCAAGTCGA AGGATCCCAA TTCGGACACG 
GACTCGGCCT TGTCCTCGGC CCCGCCCTCC ATCAGCCCGC AGCCGCCATC CAGCGGATCC 
GACAGCGGCG TGATTTGGCA AACACTGCAG GACCTGGACA AGTTGCAAAA GGAAGCGGAA 
ATGTACCAAA AGGAGAACGA ACGCCTGCAG ACGGAGGTGC AGCTGATGAA ACAGGAGCTG 
GATGCCGCGG AAAAGGCGGC CATAAGCAGG GCCAAGAAGC AGGCCCAGAT CGGGGAGCTG 
ATGCAGCGCA TCAAGGAGCT GGAAGAGATG CAGTCGAGCT TGGAGGACGA GGCCAGCGAA 
CTGAGGGAGC AGAATGAACT CCTTGAGTTT CGCATACTCG AGCTGGAAGA TGATAGCGAT 
AAGATGGAGA GCACCTGCGA GGGCCACTGC CAGAGTCTGC AGGATCTGCT GGAGCAGTCT 
GCCCAGCGGC TGGAGGATCG GGACAAGCGC TGCTTGCAAC AATTGCTGCA GTGCGTCCAG 
CAACTGGATC TGGATACCAT GATGCCAGGC GATCAGAACC GGACGGAAAC TCCCAAAAGG 
CACAATTCAA CCCACAGTCA CAGTCACAGT CACAGCCACA GTCAAAGTCA CAACCACAGG 
ATCGTAGCCA CAGTTCAGCC GTATAGCAAC TGTGCCACGC CCACAGCCCC GCCCACGCCA 
AGCAAGAGGC ACTGCAACCA GGTGAGCTCG TGGCAGAGCA GCAGCCTCAG CGAGAGCGGA 
GTGTTCGTAG AGTGCGATCT GCTTTCGAAT CCCAATCCCA TTGGCCACCC GCATCTGCAG 
TTCTATCAGG AGAGATTGGA GCAGTTGGAG GGCAAGCTGC TGATCTACGA GAGCAGCGGC 
GAGACCCAGG CACGGCACTT GGCCCAGAGA CTGCAGCGGG AACTGCAGCT GGAGAAGGAT 
CTCAAAGAGC TTGCGGACCG AGTGGAATTG CTAATCGAGC AAAACGCCCA GTTGGAGGAG 
GCCAAGTGCG AGTTTGAGGA GGCCGAGAAC GATACACGAC TCCATTTGCA GCGTAACGAG 
GTGGAACTTG AAATTCTTCG CCAGCGCAAC GTGGAGTTGG AATTCGGCAA GGAAGCACTA 
GGCGCCAAGT ATCAGGATTG CAGGGCGGAG GTACTAATCC TTCGAGAGGA TCTAGCGGCA 
GCTGAAACCC AACTGGAGCA TCTGCAGGCC GAAAGGAAGC AAGCCAGAAA GGAGCTTCAG 
GATCTGAGAA GATCGCTTCC ATTACTCCTA ATCTTCCGCT TGTTATCACT AGCCAAAATG 
GGCAACGAGG AATCCTCCCC GGGAGGCAGC CCCCGCCTCT CCTCGGGCTA CACTTCTTCT 
ATACACCAGG ATCGAGATTT CAGTGCCAAG ATCCAAACAA CGGAGTTTGA CCTCGGTCAA 
GCGGGATTTA CCGATTCCGG CGAGGAACGG GAGATCGTTT ACCTAAAGGA AGAGGTCAAG 
AGTCTGCGTA GCCAACTGAA AGAGCTAAAT GCCCGGCACT ACGAAGCCAT GGAAACAGCA 
GACTCACACT GGGTAGATCT GGAGCAGCAG TACAAGGAGC GCGAGGAGGC CCAACAGGCC 
AAGGAGGCCA GTCTCAAGCA GAAGATAGCC CAACTGCAAG ATTGCCTCCG GGAGGATTCC 
CGAGCGGCGA CCGAAAAGAT CCAGCAGCTG GAGGAGGGGG AACTGTCATT GAAGTCCTGC 
CTGGTCCGCA TGACCAAGGA GCATCGCGAC CTGCTCACCG AGAATCGCAC TCTGCAGTGC 
AGCTTGGAGA GTCTAATGGC CAAAATGGAA AAGGAGGCGG AACACAAGAT GCCCCTAACA 
GAGGCTCTAG AAAACGAGAG GCGCAAAACC CAGGCACTGA TGGATGACCT TATCTTTGCC 
AAGAAGGTGC AGCAGAACAC CGAGGATCAG TTGAGGCAGG AGACGGATGC TTTGCGCACC 
CAGATCTTCA ACATCAAAAA GGATTACCTG CACATCGAGG TCACAAATGG CGAGCTGAAA 
GAGGAGGTGG GCACGCTGGA GAACAAAATC CGCCAGATGG AGAACCAAAT GAGGGATTCG 
GAGGAGCGGG CGCGCTGCCT GGAGGACGAG CTGCGAACCA AGGACGAACA ATGCCAGCTC 
TTGGAACGCA AACTGGGCGT TATGCCCGAG GGCTACAGCC TGGCCGATGA ACTCCATGAT 
AGTCCTGCGA AGCGGGCCAA GAAGGATGAG GTACCGAGCC TCCAGGCTGC CAGCCAGGGA 
CTGGGCGTGG CCCTACTCGA TCTCGAGGGT CGTGATTTTG GTCTACCACT GCACAAGGAC 
TTCCAAGCAA TTGCCTGCAG TGTAAAGCAA CTGGCGGATA CCCTTCTCTC ACAGAGTCCG 
TCCGAGAATG CCCGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163556.2
CDS	629..4309
Translation	MAENQTKTTSSKGCNTMSRTAHILAPHNGTVVRRTDPGSKLKESFHTEKHDIEDWQTMAKNKLKRKKKLSSSLTSVGDTVNQEKRTRIEGIQLHCRGKLRPKETQSFEAEKGPSAGSQKQLGKAEKDRERPVIIGGVTTTNVIAAPPQRIKRKLQENMDTIQKLNALTEQLRLEVNELKSSLITERGAVRALRAQNDADSRRWKSEVKKLQHTLEMAKKSNGIKKAIESGPESSGAHVTADGLINYEIQRLTNEIAVLKEANRNKEEKTQLEEIKTKERNIGQLRKDLLALQSPKKLKQNEAKVPATTKPKTNPNETSQKPKKPTTKAAGETKAKTEPDTDTDISEQNPIRQAQVTPEITQLKNIELINNTKNAHKSALEMRTTMKSSQPNGLDDAPTRMRAPDEMRATTWMTTLVGSNCHSHSHSHPAVAKSKDPNSDTDSALSSAPPSISPQPPSSGSDSGVIWQTLQDLDKLQKEAEMYQKENERLQTEVQLMKQELDAAEKAAISRAKKQAQIGELMQRIKELEEMQSSLEDEASELREQNELLEFRILELEDDSDKMESTCEGHCQSLQDLLEQSAQRLEDRDKRCLQQLLQCVQQLDLDTMMPGDQNRTETPKRHNSTHSHSHSHSHSQSHNHRIVATVQPYSNCATPTAPPTPSKRHCNQVSSWQSSSLSESGVFVECDLLSNPNPIGHPHLQFYQERLEQLEGKLLIYESSGETQARHLAQRLQRELQLEKDLKELADRVELLIEQNAQLEEAKCEFEEAENDTRLHLQRNEVELEILRQRNVELEFGKEALGAKYQDCRAEVLILREDLAAAETQLEHLQAERKQARKELQDLRRSLPLLLIFRLLSLAKMGNEESSPGGSPRLSSGYTSSIHQDRDFSAKIQTTEFDLGQAGFTDSGEEREIVYLKEEVKSLRSQLKELNARHYEAMETADSHWVDLEQQYKEREEAQQAKEASLKQKIAQLQDCLREDSRAATEKIQQLEEGELSLKSCLVRMTKEHRDLLTENRTLQCSLESLMAKMEKEAEHKMPLTEALENERRKTQALMDDLIFAKKVQQNTEDQLRQETDALRTQIFNIKKDYLHIEVTNGELKEEVGTLENKIRQMENQMRDSEERARCLEDELRTKDEQCQLLERKLGVMPEGYSLADELHDSPAKRAKKDEVPSLQAASQGLGVALLDLEGRDFGLPLHKDFQAIACSVKQLADTLLSQSPSENARL

Target ORF information:

RefSeq Version	NM_001170085.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster PFTAIRE-interacting factor 1A (Pif1A), transcript variant G, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170085.2

ORF Insert Sequence:

ATGGCTGAAA ACCAAACCAA AACGACCTCT AGCAAGGGAT GTAATACAAT GAGCAGGACC 
GCCCATATCC TGGCGCCCCA CAATGGGACT GTGGTCCGCC GGACGGATCC TGGCTCCAAA 
CTCAAAGAGA GCTTCCACAC GGAGAAGCAC GATATCGAAG ACTGGCAAAC GATGGCCAAG 
AACAAGTTGA AGCGCAAGAA GAAACTGAGC AGCTCCCTGA CTTCCGTTGG GGATACGGTG 
AATCAGGAGA AGCGCACTCG CATCGAGGGC ATCCAGCTGC ATTGTCGCGG CAAATTGCGA 
CCCAAGGAGA CCCAATCCTT TGAGGCCGAG AAGGGCCCAT CTGCCGGCTC CCAGAAGCAA 
CTGGGAAAGG CAGAAAAAGA TCGAGAACGA CCCGTAATAA TTGGTGGCGT GACTACCACA 
AATGTGATAG CAGCACCTCC ACAAAGGATC AAGAGAAAAC TGCAGGAGAA CATGGACACC 
ATCCAGAAGC TAAATGCCCT CACGGAGCAA CTGCGTCTGG AGGTAAATGA ACTGAAGTCC 
AGTCTGATCA CAGAACGCGG TGCAGTGAGA GCCTTAAGGG CTCAGAATGA CGCAGATAGT 
CGTCGGTGGA AGAGTGAAGT GAAGAAGTTG CAGCATACGC TGGAAATGGC CAAGAAGAGC 
AACGGTATAA AGAAGGCCAT CGAATCGGGC CCAGAATCGA GTGGTGCACA TGTGACCGCC 
GATGGCCTCA TCAACTATGA AATCCAAAGA CTGACTAACG AGATTGCCGT GCTGAAGGAA 
GCGAATCGAA ACAAGGAGGA GAAGACCCAG TTAGAGGAAA TAAAGACCAA AGAACGTAAT 
ATAGGACAAC TGAGGAAGGA CTTGCTGGCA CTGCAGAGTC CCAAGAAGCT GAAGCAGAAT 
GAAGCCAAAG TGCCAGCGAC GACGAAACCC AAAACGAACC CCAACGAAAC GAGCCAAAAG 
CCAAAGAAAC CCACTACGAA GGCGGCGGGA GAAACAAAAG CTAAAACCGA ACCAGATACA 
GATACAGATA TATCCGAGCA AAATCCCATA CGCCAGGCTC AAGTTACACC TGAGATAACC 
CAACTCAAGA ACATTGAGCT AATCAACAAT ACGAAAAATG CACATAAATC AGCGCTCGAG 
ATGCGCACAA CAATGAAGAG CAGTCAGCCG AATGGATTGG ATGATGCTCC TACACGGATG 
AGGGCCCCCG ACGAGATGAG GGCCACCACT TGGATGACGA CGCTGGTAGG CAGTAACTGC 
CATAGCCACA GCCACAGCCA TCCGGCAGTA GCCAAGTCGA AGGATCCCAA TTCGGACACG 
GACTCGGCCT TGTCCTCGGC CCCGCCCTCC ATCAGCCCGC AGCCGCCATC CAGCGGATCC 
GACAGCGGCG TGATTTGGCA AACACTGCAG GACCTGGACA AGTTGCAAAA GGAAGCGGAA 
ATGTACCAAA AGGAGAACGA ACGCCTGCAG ACGGAGGTGC AGCTGATGAA ACAGGAGCTG 
GATGCCGCGG AAAAGGCGGC CATAAGCAGG GCCAAGAAGC AGGCCCAGAT CGGGGAGCTG 
ATGCAGCGCA TCAAGGAGCT GGAAGAGATG CAGTCGAGCT TGGAGGACGA GGCCAGCGAA 
CTGAGGGAGC AGAATGAACT CCTTGAGTTT CGCATACTCG AGCTGGAAGA TGATAGCGAT 
AAGATGGAGA GCACCTGCGA GGGCCACTGC CAGAGTCTGC AGGATCTGCT GGAGCAGTCT 
GCCCAGCGGC TGGAGGATCG GGACAAGCGC TGCTTGCAAC AATTGCTGCA GTGCGTCCAG 
CAACTGGATC TGGATACCAT GATGCCAGGC GATCAGAACC GGACGGAAAC TCCCAAAAGG 
CACAATTCAA CCCACAGTCA CAGTCACAGT CACAGCCACA GTCAAAGTCA CAACCACAGG 
ATCGTAGCCA CAGTTCAGCC GTATAGCAAC TGTGCCACGC CCACAGCCCC GCCCACGCCA 
AGCAAGAGGC ACTGCAACCA GGTGAGCTCG TGGCAGAGCA GCAGCCTCAG CGAGAGCGGA 
GTGTTCGTAG AGTGCGATCT GCTTTCGAAT CCCAATCCCA TTGGCCACCC GCATCTGCAG 
TTCTATCAGG AGAGATTGGA GCAGTTGGAG GGCAAGCTGC TGATCTACGA GAGCAGCGGC 
GAGACCCAGG CACGGCACTT GGCCCAGAGA CTGCAGCGGG AACTGCAGCT GGAGAAGGAT 
CTCAAAGAGC TTGCGGACCG AGTGGAATTG CTAATCGAGC AAAACGCCCA GTTGGAGGAG 
GCCAAGTGCG AGTTTGAGGA GGCCGAGAAC GATACACGAC TCCATTTGCA GCGTAACGAG 
GTGGAACTTG AAATTCTTCG CCAGCGCAAC GTGGAGTTGG AATTCGGCAA GGAAGCACTA 
GGCGCCAAGT ATCAGGATTG CAGGGCGGAG GTACTAATCC TTCGAGAGGA TCTAGCGGCA 
GCTGAAACCC AACTGGAGCA TCTGCAGGCC GAAAGGAAGC AAGCCAGAAA GGAGCTTCAG 
GATCTGAGAA GATCGCTTCC ATTACTCCTA ATCTTCCGCT TGTTATCACT AGCCAAAATG 
GGCAACGAGG AATCCTCCCC GGGAGGCAGC CCCCGCCTCT CCTCGGGCTA CACTTCTTCT 
ATACACCAGG ATCGAGATTT CAGTGCCAAG ATCCAAACAA CGGAGTTTGA CCTCGGTCAA 
GCGGGATTTA CCGATTCCGG CGAGGAACGG GAGATCGTTT ACCTAAAGGA AGAGGTCAAG 
AGTCTGCGTA GCCAACTGAA AGAGCTAAAT GCCCGGCACT ACGAAGCCAT GGAAACAGCA 
GACTCACACT GGGTAGATCT GGAGCAGCAG TACAAGGAGC GCGAGGAGGC CCAACAGGCC 
AAGGAGGCCA GTCTCAAGCA GAAGATAGCC CAACTGCAAG ATTGCCTCCG GGAGGATTCC 
CGAGCGGCGA CCGAAAAGAT CCAGCAGCTG GAGGAGGGGG AACTGTCATT GAAGTCCTGC 
CTGGTCCGCA TGACCAAGGA GCATCGCGAC CTGCTCACCG AGAATCGCAC TCTGCAGTGC 
AGCTTGGAGA GTCTAATGGC CAAAATGGAA AAGGAGGCGG AACACAAGAT GCCCCTAACA 
GAGGCTCTAG AAAACGAGAG GCGCAAAACC CAGGCACTGA TGGATGACCT TATCTTTGCC 
AAGAAGGTGC AGCAGAACAC CGAGGATCAG TTGAGGCAGG AGACGGATGC TTTGCGCACC 
CAGATCTTCA ACATCAAAAA GGATTACCTG CACATCGAGG TCACAAATGG CGAGCTGAAA 
GAGGAGGTGG GCACGCTGGA GAACAAAATC CGCCAGATGG AGAACCAAAT GAGGGATTCG 
GAGGAGCGGG CGCGCTGCCT GGAGGACGAG CTGCGAACCA AGGACGAACA ATGCCAGCTC 
TTGGAACGCA AACTGGGCGT TATGCCCGAG GGCTACAGCC TGGCCGATGA ACTCCATGAT 
AGTCCTGCGA AGCGGGCCAA GAAGGATGAG GTACCGAGCC TCCAGGCTGC CAGCCAGGGA 
CTGGGCGTGG CCCTACTCGA TCTCGAGGGT CGTGATTTTG GTCTACCACT GCACAAGGAC 
TTCCAAGCAA TTGCCTGCAG TGTAAAGCAA CTGGCGGATA CCCTTCTCTC ACAGAGTCCG 
TCCGAGAATG CCCGGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Pif1A cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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