Gyc76C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gyc76C
Entrez Gene ID	8674026
Full Name	Guanylyl cyclase at 76C
Synonyms	CG32215,CG42636,CG8742,DGC1,Dmel\CG42636,Dmel_CG8742,DrGC-1,GYC 76C,drgc,gim,gyc76C,l(3)76BDl,l(3)L0090
General protein information	Preferred Names Guanylyl cyclase at 76C Names CG42636 gene product from transcript CG42636-RB CG42636-PA CG42636-PB CG42636-PC CG42636-PD Gyc76C-PA Gyc76C-PB Gyc76C-PC Gyc76C-PD gimli guanylyl cyclase at 76C l(3)76BDl lethal (3) L0090 receptor-type guanylate cyclase
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 61-100

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170003.2	NP_001163474.1	guanylyl cyclase at 76C, isoform B
NM_001170002.1	NP_001163473.1	guanylyl cyclase at 76C, isoform A
NM_001170004.2	NP_001163475.1	guanylyl cyclase at 76C, isoform C
NM_001275134.1	NP_001262063.1	guanylyl cyclase at 76C, isoform D

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Gyc76C	NP_001163473.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012163	XP_320371.4
Drosophila melanogaster (fruit fly)	CG42637	NP_001007096.1
Caenorhabditis elegans (roundworm)	gcy-12	NP_494995.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These studies show that Gyc76C and downstream protein kinase G-dependent signaling regulate de novo lumen formation in the tracheal Dorsal trunk and terminal cells, most likely by affecting Arl3-mediated luminal secretion.
Title: Receptor-Type Guanylyl Cyclase at 76C (Gyc76C) Regulates De Novo Lumen Formation during Drosophila Tracheal Development.

We show that Gyc76C and the soluble cGMP-dependent kinase Foraging, likely linked by cGMP, are necessary for normal refinement and maintenance of long-range BMP signaling in the posterior crossvein
Title: The Gyc76C Receptor Guanylyl Cyclase and the Foraging cGMP-Dependent Kinase Regulate Extracellular Matrix Organization and BMP Signaling in the Developing Wing of Drosophila melanogaster.

Gyc76C genetically interacts with Semaphorin 1a and physically associates with the Sema-1a receptor plexin A.
Title: Function of the Drosophila receptor guanylyl cyclase Gyc76C in PlexA-mediated motor axon guidance.

The following Gyc76C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gyc76C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa19006	NM_001170004.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant C (Gyc76C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa19006	NM_001275134.1 Latest version!	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant D (Gyc76C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa19006	NM_001170003.2 Latest version!	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant B (Gyc76C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa19006	NM_001170002.1 Latest version!	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant A (Gyc76C), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gyc76C ( NM_001170003.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163474.1 Drosophila melanogaster guanylyl cyclase at 76C, transcript variant B (Gyc76C), mRNA.

Price & Availability↑

CloneID	OFa19006
Clone ID Related Accession (Same CDS sequence)	NM_001170003.2 , NM_001170002.1 , NM_001170004.2 , NM_001275134.1
Accession Version	NM_001170003.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 76C, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001170003.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163474.1
CDS	706..5283
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGDLKTRQGLAISGALTMALDEVNKDPNLLPNVYLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWSPVADLLKDQATKRNMTINHKQSFIDNRVKCCEQMLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRRVDQITFMSNCHSTENFNQMARSLLVVASTPPTKDYIQFTKQVQKYSSKPPFNLEIPRLFVESNFSKFISIYAAYLYDSVKLYAWAVDKMLREETRVLTDDVIFEVASNGTRVIDTIIKNRTYMSITGSKIKIDQYGDSEGNFSVLAYKPHKWNNSNNMPCNYHMVPVAYFHQGEEHPEYKLINGSIDWPSGGEKPADEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLVYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQINFEPKEIVDYVKKLPLKGEDPFRPEVESIIEAESCPDYVLACIRDCWAEDPEERPEFSVIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKMKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLHAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVVTWWLTGANENAIQKKLVDMMDMPPPLFSRPRKSPKLNPDSRQPSIQAMHFCGTGSRRQSTVPRAMDGESTYSLQGSVRESPRMVSKRDRDRERPPINGLGAGHFVGGALLESAQASLSTLNHSETNETNCDMDGGSGGVSGSGSGLVRQPNALHKPLAMVRPHRIISAAQLPQLGDNDDDSADTLLRESRSLDPMPMQQLRKRHDRVKLPPSKLSKNNSRSLDTGVSLISGNPNGEVHSSQLDLDNEMTANPVDATDGYDDELGLLMRHDNGQLPALRYSGSFPNAQISIVPTGRSAGGGGGGREGGGSNCAKHLNNNCNGGVNVEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMDGPGGHGGNSVSYAADIDNRHPGDLDFFSGSSNQHHRSKAAGGSKLTNWMTNIFKGNGVRSGEARRVGILPSGVHGARTGFTDMAASAAARDRESIV

Target ORF information:

RefSeq Version	NM_001170003.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant B (Gyc76C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170003.2

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc76C ( NM_001170002.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163473.1 Drosophila melanogaster guanylyl cyclase at 76C, transcript variant A (Gyc76C), mRNA.

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CloneID	OFa19006
Clone ID Related Accession (Same CDS sequence)	NM_001170003.2 , NM_001170002.1 , NM_001170004.2 , NM_001275134.1
Accession Version	NM_001170002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 76C, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163473.1
CDS	748..5325
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGDLKTRQGLAISGALTMALDEVNKDPNLLPNVYLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWSPVADLLKDQATKRNMTINHKQSFIDNRVKCCEQMLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRRVDQITFMSNCHSTENFNQMARSLLVVASTPPTKDYIQFTKQVQKYSSKPPFNLEIPRLFVESNFSKFISIYAAYLYDSVKLYAWAVDKMLREETRVLTDDVIFEVASNGTRVIDTIIKNRTYMSITGSKIKIDQYGDSEGNFSVLAYKPHKWNNSNNMPCNYHMVPVAYFHQGEEHPEYKLINGSIDWPSGGEKPADEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLVYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQINFEPKEIVDYVKKLPLKGEDPFRPEVESIIEAESCPDYVLACIRDCWAEDPEERPEFSVIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKMKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLHAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVVTWWLTGANENAIQKKLVDMMDMPPPLFSRPRKSPKLNPDSRQPSIQAMHFCGTGSRRQSTVPRAMDGESTYSLQGSVRESPRMVSKRDRDRERPPINGLGAGHFVGGALLESAQASLSTLNHSETNETNCDMDGGSGGVSGSGSGLVRQPNALHKPLAMVRPHRIISAAQLPQLGDNDDDSADTLLRESRSLDPMPMQQLRKRHDRVKLPPSKLSKNNSRSLDTGVSLISGNPNGEVHSSQLDLDNEMTANPVDATDGYDDELGLLMRHDNGQLPALRYSGSFPNAQISIVPTGRSAGGGGGGREGGGSNCAKHLNNNCNGGVNVEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMDGPGGHGGNSVSYAADIDNRHPGDLDFFSGSSNQHHRSKAAGGSKLTNWMTNIFKGNGVRSGEARRVGILPSGVHGARTGFTDMAASAAARDRESIV

Target ORF information:

RefSeq Version	NM_001170002.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant A (Gyc76C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170002.1

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc76C ( NM_001170004.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163475.1 Drosophila melanogaster guanylyl cyclase at 76C, transcript variant C (Gyc76C), mRNA.

Price & Availability↑

CloneID	OFa19006
Clone ID Related Accession (Same CDS sequence)	NM_001170003.2 , NM_001170002.1 , NM_001170004.2 , NM_001275134.1
Accession Version	NM_001170004.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 76C, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001170004.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163475.1
CDS	869..5446
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGDLKTRQGLAISGALTMALDEVNKDPNLLPNVYLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWSPVADLLKDQATKRNMTINHKQSFIDNRVKCCEQMLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRRVDQITFMSNCHSTENFNQMARSLLVVASTPPTKDYIQFTKQVQKYSSKPPFNLEIPRLFVESNFSKFISIYAAYLYDSVKLYAWAVDKMLREETRVLTDDVIFEVASNGTRVIDTIIKNRTYMSITGSKIKIDQYGDSEGNFSVLAYKPHKWNNSNNMPCNYHMVPVAYFHQGEEHPEYKLINGSIDWPSGGEKPADEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLVYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQINFEPKEIVDYVKKLPLKGEDPFRPEVESIIEAESCPDYVLACIRDCWAEDPEERPEFSVIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKMKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLHAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVVTWWLTGANENAIQKKLVDMMDMPPPLFSRPRKSPKLNPDSRQPSIQAMHFCGTGSRRQSTVPRAMDGESTYSLQGSVRESPRMVSKRDRDRERPPINGLGAGHFVGGALLESAQASLSTLNHSETNETNCDMDGGSGGVSGSGSGLVRQPNALHKPLAMVRPHRIISAAQLPQLGDNDDDSADTLLRESRSLDPMPMQQLRKRHDRVKLPPSKLSKNNSRSLDTGVSLISGNPNGEVHSSQLDLDNEMTANPVDATDGYDDELGLLMRHDNGQLPALRYSGSFPNAQISIVPTGRSAGGGGGGREGGGSNCAKHLNNNCNGGVNVEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMDGPGGHGGNSVSYAADIDNRHPGDLDFFSGSSNQHHRSKAAGGSKLTNWMTNIFKGNGVRSGEARRVGILPSGVHGARTGFTDMAASAAARDRESIV

Target ORF information:

RefSeq Version	NM_001170004.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant C (Gyc76C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170004.2

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gyc76C ( NM_001275134.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001262063.1 Drosophila melanogaster guanylyl cyclase at 76C, transcript variant D (Gyc76C), mRNA.

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CloneID	OFa19006
Clone ID Related Accession (Same CDS sequence)	NM_001170003.2 , NM_001170002.1 , NM_001170004.2 , NM_001275134.1
Accession Version	NM_001275134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	guanylyl cyclase at 76C, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001262063.1
CDS	732..5309
Translation	MTRWPFNLLLLLSVAVRDCSNHRTVLTVGYLTALTGDLKTRQGLAISGALTMALDEVNKDPNLLPNVYLDLRWNDTKGDTVLATKAITEMICDGIATIFGPEGPCYVEAIVSQSRNIPMISYKCAEYRASAIPTFARTEPPDTQVVKSLLALLRYYAWNKFSILYEDVWSPVADLLKDQATKRNMTINHKQSFIDNRVKCCEQMLDCCRSGYWYQLVQNTMNRTRIYVFLGAANSLVDFMSSMETAGLFARGEYMVIFVDMMVYSEREAEKYLRRVDQITFMSNCHSTENFNQMARSLLVVASTPPTKDYIQFTKQVQKYSSKPPFNLEIPRLFVESNFSKFISIYAAYLYDSVKLYAWAVDKMLREETRVLTDDVIFEVASNGTRVIDTIIKNRTYMSITGSKIKIDQYGDSEGNFSVLAYKPHKWNNSNNMPCNYHMVPVAYFHQGEEHPEYKLINGSIDWPSGGEKPADEPMCGFANELCKKDDTHYTSTVAAVVLGVLLFCSGVITMSIYRKWKIELEIEGLLWKIDPNEIKGYSGNEIVSSPSKVSLMSAQSYGSRWTNQFVTSTGRLRGAVVRIKELKFPRKRDISREIMKEMRLLRELRHDNINSFIGASVEPTRILLVTDYCAKGSLYDIIENEDIKLDDLFIASLIHDLIKGMIYIHNSQLVYHGNLKSSNCVVTSRWMLQVTDFGLHELRQCAENESIGEHQHYRNQLWRAPELLRNHIHGSQKGDVYAFAIIMYEIFSRKGPFGQINFEPKEIVDYVKKLPLKGEDPFRPEVESIIEAESCPDYVLACIRDCWAEDPEERPEFSVIRNRLKKMRGGKTKNIMDQMMEMMEKYANNLEDIVTERTRLLCEEKMKTEDLLHRMLPQSVAEKLTMGQGVEPVSYDLVTIYFSDIVGFTAMSAESTPLQVVNFLNDLYTVFDRIIRGYDVYKVETIGDAYMVVSGLPIKNGDRHAGEIASMALELLHAVKQHRIAHRPNETLKLRIGMHTGPVVAGVVGLTMPRYCLFGDTVNTASRMESNGEALKIHISNKCKLALDKLGGGYITEKRGLVNMKGKGDVVTWWLTGANENAIQKKLVDMMDMPPPLFSRPRKSPKLNPDSRQPSIQAMHFCGTGSRRQSTVPRAMDGESTYSLQGSVRESPRMVSKRDRDRERPPINGLGAGHFVGGALLESAQASLSTLNHSETNETNCDMDGGSGGVSGSGSGLVRQPNALHKPLAMVRPHRIISAAQLPQLGDNDDDSADTLLRESRSLDPMPMQQLRKRHDRVKLPPSKLSKNNSRSLDTGVSLISGNPNGEVHSSQLDLDNEMTANPVDATDGYDDELGLLMRHDNGQLPALRYSGSFPNAQISIVPTGRSAGGGGGGREGGGSNCAKHLNNNCNGGVNVEDDLESPLLQRQASLSVPPEEMLAHNKRWHSLEHMDGPGGHGGNSVSYAADIDNRHPGDLDFFSGSSNQHHRSKAAGGSKLTNWMTNIFKGNGVRSGEARRVGILPSGVHGARTGFTDMAASAAARDRESIV

Target ORF information:

RefSeq Version	NM_001275134.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster guanylyl cyclase at 76C, transcript variant D (Gyc76C), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001275134.1

ORF Insert Sequence:

ATGACGCGTT GGCCCTTTAA TCTACTACTC TTGCTGTCGG TGGCGGTACG CGACTGTAGC 
AATCATCGCA CCGTCCTCAC TGTCGGCTAC CTAACGGCCC TCACTGGCGA TCTCAAGACG 
CGTCAGGGAC TGGCCATTTC CGGTGCCCTG ACTATGGCCC TGGATGAGGT CAACAAAGAT 
CCCAATCTTC TGCCAAATGT GTACCTGGAT CTGCGCTGGA ATGACACCAA GGGGGACACG 
GTACTGGCCA CCAAGGCCAT CACCGAGATG ATATGCGATG GCATCGCCAC CATTTTTGGC 
CCGGAAGGAC CTTGCTATGT GGAGGCCATC GTCTCGCAGA GTCGAAACAT TCCAATGATT 
TCCTACAAAT GTGCAGAGTA CCGTGCATCC GCCATACCGA CATTCGCCCG CACCGAGCCG 
CCAGATACAC AGGTCGTTAA GTCGCTGCTT GCCCTTCTGC GATATTATGC GTGGAATAAG 
TTTTCCATTC TGTACGAGGA TGTGTGGAGT CCGGTGGCTG ATCTGCTGAA AGATCAGGCC 
ACCAAGAGAA ATATGACGAT CAACCACAAG CAGTCGTTCA TTGATAATCG AGTCAAGTGC 
TGTGAGCAGA TGCTGGACTG CTGTCGTTCT GGGTATTGGT ACCAGCTGGT GCAAAACACG 
ATGAATCGCA CACGAATCTA CGTATTTCTG GGGGCGGCGA ATAGTCTGGT GGACTTTATG 
AGTTCCATGG AGACCGCTGG TCTGTTTGCC CGTGGCGAGT ATATGGTGAT CTTTGTCGAC 
ATGATGGTCT ATTCCGAGAG GGAGGCAGAA AAGTACTTGC GCAGAGTGGA TCAAATAACA 
TTCATGTCCA ATTGCCATAG CACGGAGAAC TTCAATCAAA TGGCCCGCAG TCTTCTCGTC 
GTGGCATCCA CGCCACCCAC AAAGGATTAC ATACAGTTCA CTAAACAAGT GCAGAAGTAC 
AGCTCAAAGC CGCCGTTCAA TTTGGAAATT CCCAGGCTGT TTGTAGAAAG TAATTTTAGC 
AAGTTCATAT CGATCTATGC CGCGTACCTG TACGATTCGG TAAAGCTGTA TGCCTGGGCT 
GTGGACAAAA TGCTGCGGGA GGAGACCCGA GTCTTGACCG ATGATGTGAT CTTCGAGGTG 
GCCAGCAACG GAACACGTGT AATCGATACC ATAATCAAAA ACCGTACTTA TATGAGCATA 
ACTGGATCCA AAATTAAGAT CGATCAATAT GGCGATTCGG AGGGTAATTT TTCGGTCCTG 
GCCTACAAGC CACACAAGTG GAATAACTCG AACAACATGC CCTGCAACTA TCACATGGTT 
CCTGTAGCTT ACTTTCATCA AGGCGAAGAA CATCCAGAGT ACAAGCTCAT CAATGGCTCC 
ATAGATTGGC CATCGGGCGG TGAGAAGCCC GCAGATGAAC CGATGTGCGG TTTTGCAAAC 
GAGCTGTGTA AAAAGGACGA CACCCACTAT ACGTCCACTG TCGCCGCTGT GGTTTTGGGC 
GTATTGCTCT TCTGCTCAGG TGTTATCACC ATGAGTATAT ATCGGAAGTG GAAGATCGAG 
TTGGAGATTG AGGGCTTACT CTGGAAGATC GATCCGAACG AAATAAAGGG TTATTCTGGC 
AACGAGATTG TCTCCTCACC GAGCAAAGTC AGTTTGATGA GTGCCCAGAG CTATGGTTCC 
CGCTGGACCA ACCAGTTTGT TACCTCCACG GGTCGCCTTC GCGGGGCCGT GGTTCGTATT 
AAGGAGTTGA AATTCCCGCG GAAGCGAGAC ATATCCAGGG AGATCATGAA GGAGATGCGA 
TTGTTGCGCG AACTGCGTCA CGATAACATC AACAGCTTCA TTGGCGCCAG CGTGGAGCCA 
ACACGCATTC TTCTAGTCAC CGATTACTGT GCCAAAGGCA GTCTTTACGA CATCATTGAG 
AACGAGGACA TTAAGTTGGA CGATCTATTC ATCGCCTCGC TTATTCACGA CCTCATAAAG 
GGCATGATCT ATATACACAA CTCTCAGCTC GTCTATCATG GTAACCTTAA ATCCTCAAAC 
TGTGTGGTCA CCTCGCGTTG GATGCTCCAA GTCACCGATT TCGGGCTGCA CGAGTTGCGG 
CAGTGTGCCG AAAACGAATC CATCGGGGAG CATCAGCATT ATCGAAATCA ACTTTGGCGC 
GCTCCAGAGC TGCTGCGGAA TCACATTCAC GGCAGCCAGA AGGGCGATGT CTATGCCTTT 
GCCATCATCA TGTACGAGAT ATTTAGTCGT AAGGGTCCGT TCGGACAAAT TAACTTTGAA 
CCAAAGGAAA TCGTAGACTA TGTCAAGAAG CTGCCGCTCA AGGGAGAGGA TCCCTTTCGG 
CCGGAAGTGG AGTCCATTAT AGAGGCCGAG TCCTGTCCGG ATTACGTACT GGCCTGCATC 
AGGGACTGCT GGGCTGAAGA TCCCGAAGAG CGGCCCGAAT TCAGTGTCAT ACGCAATCGC 
TTGAAGAAGA TGCGTGGTGG TAAGACCAAG AACATCATGG ATCAAATGAT GGAAATGATG 
GAGAAGTATG CCAACAACTT GGAGGACATT GTCACCGAGC GCACGCGTCT CCTGTGCGAG 
GAAAAGATGA AAACGGAGGA TCTCCTGCAT CGCATGCTAC CGCAATCGGT GGCTGAAAAA 
TTGACCATGG GTCAGGGTGT CGAGCCCGTT TCATATGATT TGGTAACCAT ATATTTTAGC 
GACATTGTTG GTTTTACTGC AATGTCGGCG GAGAGCACAC CGCTGCAAGT GGTGAACTTT 
TTGAATGATC TCTACACAGT GTTCGATCGC ATCATACGCG GCTATGATGT CTATAAAGTT 
GAAACCATTG GAGATGCTTA CATGGTGGTT TCGGGTCTGC CAATTAAGAA CGGAGATCGA 
CATGCAGGCG AGATAGCTTC GATGGCCCTG GAGCTGCTCC ACGCTGTCAA GCAGCACCGC 
ATAGCCCATC GTCCCAACGA GACGCTGAAG CTGCGCATTG GAATGCACAC GGGTCCGGTG 
GTAGCGGGCG TGGTTGGTCT CACCATGCCG AGATACTGCC TTTTTGGTGA CACCGTGAAT 
ACAGCGTCCC GGATGGAGAG CAACGGAGAG GCGCTAAAGA TTCACATTTC CAATAAGTGC 
AAGCTGGCGC TGGACAAACT GGGTGGTGGC TACATAACCG AGAAACGTGG CCTGGTCAAT 
ATGAAGGGCA AGGGCGATGT GGTTACTTGG TGGCTAACCG GAGCCAACGA GAATGCTATT 
CAGAAGAAGT TAGTGGATAT GATGGACATG CCGCCGCCGC TGTTTAGTCG ACCTCGAAAG 
AGTCCAAAGC TAAATCCCGA CTCCCGTCAG CCAAGCATCC AGGCCATGCA CTTCTGCGGC 
ACCGGATCAA GACGCCAGAG CACAGTTCCC AGGGCGATGG ACGGCGAATC AACGTATAGC 
CTCCAGGGAT CCGTGAGGGA ATCACCGCGC ATGGTCAGCA AACGTGACAG GGATCGAGAG 
CGACCCCCCA TCAATGGACT GGGCGCAGGG CACTTCGTGG GGGGAGCTCT CCTCGAATCC 
GCACAGGCCT CGCTTAGCAC GCTGAATCAT TCCGAAACAA ACGAAACTAA CTGTGATATG 
GATGGGGGAT CGGGAGGAGT GTCAGGATCT GGATCTGGAT TAGTGCGTCA ACCCAATGCA 
CTGCACAAAC CGTTGGCCAT GGTGCGGCCG CACCGGATTA TTAGTGCTGC CCAGCTACCC 
CAACTGGGAG ATAACGATGA TGACTCCGCA GACACGCTGT TGCGGGAGTC TCGCTCCCTG 
GATCCCATGC CCATGCAGCA ACTGCGCAAA CGTCACGACA GGGTAAAGTT GCCGCCCTCG 
AAGTTGTCCA AGAACAATTC CCGATCGCTG GACACGGGTG TATCGCTGAT CAGTGGGAAT 
CCCAACGGTG AAGTGCACTC GAGTCAACTG GATTTGGATA ACGAGATGAC CGCCAACCCG 
GTGGATGCCA CCGATGGCTA CGACGATGAG CTGGGCCTTC TGATGCGCCA CGACAACGGC 
CAACTGCCTG CTCTCCGCTA TTCAGGCAGC TTTCCCAACG CTCAGATTAG CATTGTTCCT 
ACAGGAAGGT CAGCAGGAGG AGGAGGAGGA GGAAGAGAAG GAGGTGGGAG CAACTGTGCC 
AAGCATCTGA ACAACAACTG CAACGGAGGA GTGAACGTCG AGGATGATCT TGAGTCGCCC 
CTGCTGCAAC GCCAGGCATC GCTGTCGGTG CCGCCAGAGG AGATGCTGGC GCACAACAAA 
CGTTGGCACT CACTGGAGCA CATGGATGGT CCCGGTGGGC ATGGCGGAAA TAGCGTTAGC 
TATGCCGCCG ATATTGATAA CCGCCACCCC GGAGATCTGG ACTTCTTCAG CGGTTCCTCC 
AATCAGCATC ATAGATCCAA AGCGGCTGGT GGGAGTAAGC TGACTAACTG GATGACTAAC 
ATCTTCAAGG GCAACGGCGT GCGTAGCGGT GAGGCTAGGC GAGTAGGAAT CCTGCCCAGC 
GGTGTGCACG GCGCACGAAC CGGATTCACG GATATGGCGG CATCGGCAGC AGCCCGCGAT 
CGTGAGAGTA TAGTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Gyc76C cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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