robls54B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	robls54B
Entrez Gene ID	5740248
Full Name	roadblock similar 54B
Synonyms	CG34192,Dmel\CG34192,GENE 3,HDC06929,gene 3
General protein information	Preferred Names roadblock similar 54B Names CG34192 gene product from transcript CG34192-RB CG34192-PA CG34192-PB robls54B-PA robls54B-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 54B16-54B16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001103886.2	NP_001097356.1	roadblock similar 54B, isoform A
NM_001299599.1	NP_001286528.1	roadblock similar 54B, isoform B

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	robls54B	NP_001097356.1
Drosophila melanogaster (fruit fly)	CG10822	NP_611450.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following robls54B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the robls54B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa11875	NM_001299599.1 Latest version!	Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
OFa11875	NM_001103886.2 Latest version!	Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

robls54B ( NM_001103886.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001097356.1 Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa11875
Clone ID Related Accession (Same CDS sequence)	NM_001103886.2 , NM_001299599.1
Accession Version	NM_001103886.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roadblock similar 54B, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_001103886.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCGG ATCTTCCACA AAAGCCACCT CGAACTCTAC GATATGTGGA AAAAGCATTC 
GACCTGGTCA AATCGAAAAA ACATGTTCGA GATGTAGTGA TCCTCAACGA GTCGGGACAT 
CCGGTGATGA GTACGATGGA CCGAGAGGAT GCTGTCCAAT TTTCGGGACC TTTTCAGGCT 
ATTCGAGGAA GACTGGAACG CGGTATGAGT AAAATAGATC CCACGGACGA ACTACTAATG 
CTGAGAATAC GCACAAGAAC CAACGAGGTG CTCCTGGTGC CAGACTCCAA GATAACACTA 
CTCGTAGTAC AGAACGCGCA TGATTACTTC GAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001097356.1
CDS	37..375
Translation	MFPDLPQKPPRTLRYVEKAFDLVKSKKHVRDVVILNESGHPVMSTMDREDAVQFSGPFQAIRGRLERGMSKIDPTDELLMLRIRTRTNEVLLVPDSKITLLVVQNAHDYFEK

Target ORF information:

RefSeq Version	NM_001103886.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001103886.2

ORF Insert Sequence:

ATGTTTCCGG ATCTTCCACA AAAGCCACCT CGAACTCTAC GATATGTGGA AAAAGCATTC 
GACCTGGTCA AATCGAAAAA ACATGTTCGA GATGTAGTGA TCCTCAACGA GTCGGGACAT 
CCGGTGATGA GTACGATGGA CCGAGAGGAT GCTGTCCAAT TTTCGGGACC TTTTCAGGCT 
ATTCGAGGAA GACTGGAACG CGGTATGAGT AAAATAGATC CCACGGACGA ACTACTAATG 
CTGAGAATAC GCACAAGAAC CAACGAGGTG CTCCTGGTGC CAGACTCCAA GATAACACTA 
CTCGTAGTAC AGAACGCGCA TGATTACTTC GAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

robls54B ( NM_001299599.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286528.1 Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa11875
Clone ID Related Accession (Same CDS sequence)	NM_001103886.2 , NM_001299599.1
Accession Version	NM_001299599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	roadblock similar 54B, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTCCGG ATCTTCCACA AAAGCCACCT CGAACTCTAC GATATGTGGA AAAAGCATTC 
GACCTGGTCA AATCGAAAAA ACATGTTCGA GATGTAGTGA TCCTCAACGA GTCGGGACAT 
CCGGTGATGA GTACGATGGA CCGAGAGGAT GCTGTCCAAT TTTCGGGACC TTTTCAGGCT 
ATTCGAGGAA GACTGGAACG CGGTATGAGT AAAATAGATC CCACGGACGA ACTACTAATG 
CTGAGAATAC GCACAAGAAC CAACGAGGTG CTCCTGGTGC CAGACTCCAA GATAACACTA 
CTCGTAGTAC AGAACGCGCA TGATTACTTC GAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286528.1
CDS	202..540
Translation	MFPDLPQKPPRTLRYVEKAFDLVKSKKHVRDVVILNESGHPVMSTMDREDAVQFSGPFQAIRGRLERGMSKIDPTDELLMLRIRTRTNEVLLVPDSKITLLVVQNAHDYFEK

Target ORF information:

RefSeq Version	NM_001299599.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster roadblock similar 54B (robls54B), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299599.1

ORF Insert Sequence:

ATGTTTCCGG ATCTTCCACA AAAGCCACCT CGAACTCTAC GATATGTGGA AAAAGCATTC 
GACCTGGTCA AATCGAAAAA ACATGTTCGA GATGTAGTGA TCCTCAACGA GTCGGGACAT 
CCGGTGATGA GTACGATGGA CCGAGAGGAT GCTGTCCAAT TTTCGGGACC TTTTCAGGCT 
ATTCGAGGAA GACTGGAACG CGGTATGAGT AAAATAGATC CCACGGACGA ACTACTAATG 
CTGAGAATAC GCACAAGAAC CAACGAGGTG CTCCTGGTGC CAGACTCCAA GATAACACTA 
CTCGTAGTAC AGAACGCGCA TGATTACTTC GAAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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robls54B cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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